CN105004767A - Phenylthiocarbamide solution concentration detecting device and method - Google Patents

Abstract

The invention discloses a phenylthiocarbamide solution concentration detecting device and method. The device comprises a computer, a collecting unit and a collecting workbench. The collecting unit comprises a cell electrode plate, an acoustic surface wave resonator and a conversion unit. The collecting workbench comprises a base, the base is provided with a containing groove and a lifting device, a liquid dropping chamber is formed above the containing groove and connected with the lifting device through a connecting mechanism, a micro pump communicated with the liquid dropping chamber is arranged at the bottom of the liquid dropping chamber, a liquid dropping head communicated with the micro pump is arranged at the bottom of the micro pump, a liquid inlet is formed in the top of the liquid dropping chamber, a washing mechanism and a draining mechanism are arranged in the liquid dropping chamber, a disk is arranged above the liquid dropping chamber, a plurality of liquid storage cavities are formed around the circle center in the disk, a liquid outlet corresponding to the liquid inlet in position is formed in the bottom of the corresponding liquid storage cavity, a liquid outlet electromagnetic valve is arranged at the liquid outlet, and a driving motor driving the disk to rotate is arranged on the top of the liquid dropping chamber. According to the phenylthiocarbamide solution concentration detecting device and method, the structure is simple, cost is low, and the phenylthiocarbamide solution concentration can be quickly and accurately detected.

Description

A kind of phenylthiourea solution concentration detector and method

Technical field

The present invention relates to solution concentration detection technical field, particularly relate to a kind of phenylthiourea solution concentration detector and method.

Background technology

Existing phenylthiourea solution concentration detection technical method has instrumental analysis detection method and chemical measure, although instrumental analysis detection method is simple to operate, there is the defect that equipment volume is large, somewhat expensive, accuracy of detection are low; There is complex operation in chemical measure, the deficiency of poor repeatability.

Summary of the invention

The object of the invention is to overcome the technical matters that existing phenylthiourea solution concentration detector cost is high, accuracy of detection is lower, provide a kind of phenylthiourea solution concentration detector and method, its structure is simple, and cost is low, can detect the concentration of phenylthiourea solution fast and accurately.

In order to solve the problem, the present invention is achieved by the following technical solutions:

A kind of phenylthiourea solution concentration detector of the present invention, comprise computing machine, collecting unit and collecting work platform, described collecting unit comprises cell battery lead plate, SAW (Surface Acoustic Wave) resonator and converting unit, described collecting work platform comprises base, described base is provided with standing groove and lifter, drip chamber is provided with above described standing groove, described drip chamber is connected with lifter by bindiny mechanism, the micro pump be communicated with drip chamber is provided with bottom described drip chamber, the liquid droping head be communicated with micro pump is provided with bottom described micro pump, described drip chamber top is provided with inlet, wiper mechanism and drainage mechanism is also provided with in described drip chamber, disk is provided with above described drip chamber, described disk is provided with several liquid storage cylinders around the center of circle, position corresponding with inlet bottom described liquid storage cylinder is provided with liquid outlet, described liquid outlet is provided with liquid outlet electromagnetic valve, described drip chamber top is provided with the drive motor that driver plate rotates, described SAW (Surface Acoustic Wave) resonator is electrically connected with cell battery lead plate and converting unit respectively, described computing machine respectively with converting unit, micro pump, wiper mechanism, drainage mechanism, liquid outlet electromagnetic valve, drive motor and lifter electrical connection.

In the technical program, obtain the phenylthiourea solution of variable concentrations in advance, the phenylthiourea solution of variable concentrations is poured into respectively in each liquid storage cylinder on disk, cell battery lead plate is placed in standing groove, and cell battery lead plate is positioned at below liquid droping head.Lifter controls drip chamber lifting, thus the position of adjustment liquid droping head, make the distance between liquid droping head and cell battery lead plate be optimum value.

Start the work of phenylthiourea solution concentration detector, liquid outlet electromagnetic valve on liquid storage cylinder corresponding with drip chamber on computer controlled rounding dish opens (aiming at the inlet on drip chamber with the liquid outlet on the liquid storage cylinder that drip chamber is corresponding), and the phenylthiourea solution in liquid storage cylinder injects drip chamber by inlet.Then computing machine controls micro pump work, and micro pump controls liquid droping head at interval of 2.1 seconds dropping liquids once, and each dropping liquid amount is 0.05ml, altogether dropping liquid 260 times.Phenylthiourea solution in drip chamber drips on cell battery lead plate by liquid droping head, cell battery lead plate produces specificity current-responsive, current-responsive is captured by SAW (Surface Acoustic Wave) resonator and outputs to converting unit in the frequency form, converting unit detects frequency signal, and output frequency response curve is to computing machine.The frequency response curve up-sampling that computing machine is receiving, obtains 120 sampled values, using sampled value as input data S (t), and input second-order linear system accidental resonance model $\frac{d^{2}x\left(t\right)}{{\mathrm{dt}}^2}+[{4r}^2+2\mathrm{\ξ}\left(t\right)+b{\mathrm{\ξ}}^2\left(t\right)]\frac{\mathrm{dx}\left(t\right)}{\mathrm{dt}}+{\mathrm{\ω}}^{4}x\left(t\right)=A\cos \left(\mathrm{\Ωt}\right)+\mathrm{cS}\left(t\right)$ In, and second-order linear system accidental resonance model is resonated, obtain the output signal-to-noise ratio of this phenylthiourea solution $\mathrm{SNR}=\frac{\mathrm{r\λ\Ω}+{2\mathrm{qa}}^2\mathrm{bc}({\mathrm{\Ω}}^3-\mathrm{\Ω})}{1+{2\mathrm{qa}}^{2}b+5r-a^2}.$

After dropping liquid terminates, phenylthiourea solution remaining in drip chamber is discharged by drainage mechanism, and wiper mechanism carries out cleaning to drip chamber and dries up.Then, computing machine rotates clockwise certain angle by drive motor driver plate, next liquid storage cylinder is made to turn to the position corresponding with drip chamber, then the output signal-to-noise ratio that above-mentioned steps detects phenylthiourea solution in this liquid storage cylinder is repeated, then phenylthiourea solution remaining in drip chamber is discharged by drainage mechanism, and wiper mechanism carries out cleaning to drip chamber and dries up.Circulation like this is until detect the output signal-to-noise ratio of the phenylthiourea solution on disk in all liquid storage cylinders, and the concentration of the phenylthiourea solution obtained in advance is respectively k ₁, k ₂..., k _n, the output signal-to-noise ratio of its correspondence is respectively SNR ₁, SNR ₂..., SNR _n, computing machine is by point (k ₁, SNR ₁), (k ₂, SNR ₂) ..., (k _n, SNR _n) fit to straight line, the forecast model of phenylthiourea solution is obtained according to the straight line of matching:

When detecting the phenylthiourea solution to be measured of unknown concentration, as long as phenylthiourea solution to be measured is poured into in liquid storage cylinder corresponding with drip chamber on disk, repeat the output signal-to-noise ratio SNR that above-mentioned steps obtains this phenylthiourea solution, this output signal-to-noise ratio SNR substituted into the forecast model of phenylthiourea solution: thus calculate the concentration k of phenylthiourea solution to be measured.Structure of the present invention is simple, cost is low, after each dropping liquid completes, wiper mechanism can carry out cleaning to drip chamber and dry up, the concentration ensureing next time to inject the phenylthiourea solution of drip chamber can not be subject to impurity interference, and improve accuracy of detection, pick-up unit controls automatic operation by computing machine, detection speed is fast, improves detection efficiency.

As preferably, described cell battery lead plate comprises substrate, described substrate is provided with working electrode, to electrode and two contrast electrodes, described working electrode comprises the rounded portions of front end, this rounded portions is made up of foam copper, this foam copper is coated with one deck Gold plated Layer, described Gold plated Layer is provided with mouse bacterium shape taste bud taste receptor cells, described to the front end of electrode with the front end of contrast electrode is curved is centered around outside rounded portions, described working electrode front end, painting layer is scribbled to electrode front end and contrast electrode front end outer side.To electrode, the upper surface of contrast electrode and working electrode is all higher than upper surface of base plate 0.5 to 1 centimetre, substrate is made up of ST cut type quartz, electrode and contrast electrode are made up of tungsten, working electrode is made up of foam copper material, foam copper is equally distributed three-dimensional netted pore structure, it has the reticulate texture of multi-layer stable and connects closely, reticulate texture is not yielding and subside, this makes cell can enter foam copper inside configuration, cell can better be attached on electrode, it is lower that Gold plated Layer compares foam copper toxicity, make cytoactive better with cells contacting, also make detection sensitivity better.

As preferably, described converting unit comprises oscillatory circuit and frequency detector, and described SAW (Surface Acoustic Wave) resonator is electrically connected with frequency detector by oscillatory circuit, and described frequency detector is electrically connected with computing machine.

As preferably, described wiper mechanism comprises air intake opening and water inlet, described drip chamber is communicated with water inlet with air intake opening by a connecting line, described air intake opening is connected with air pump, described water inlet is connected with water pump, and described water pump is connected with water tank, and described air intake opening is provided with air inlet electromagnetic valve, described water inlet is provided with entering water electromagnetic valve, and described air pump, water pump, air inlet electromagnetic valve and entering water electromagnetic valve are electrically connected with computing machine respectively.When drip chamber is cleaned, first close air inlet electromagnetic valve, start water pump, open entering water electromagnetic valve, water pump is to injected clear water cleaning in drip chamber, then water is discharged by drainage mechanism, switch off the pump and entering water electromagnetic valve, booster air pump, unlatching air inlet electromagnetic valve, air pump passes into air and is dried up by drip chamber in drip chamber.

As preferably, described wiper mechanism also comprises the rabbling mechanism be arranged in drip chamber, described rabbling mechanism comprises the vertical pole of the cross bar of horizontally disposed hollow and the hollow of longitudinally setting, the bottom in described cross bar stage casing is located at the top of vertical pole by bearing holder (housing, cover), cavity in described cross bar and the cavity connects in vertical pole, the leading flank of described cross bar one end and the trailing flank of the cross bar other end are provided with several through holes, described through hole is communicated with cross bar internal cavity, described vertical pole is arranged on bottom drip chamber, cavity in described vertical pole is communicated with water inlet with air intake opening by connecting line.During cleaning, clear water passes into cross bar from vertical pole, and discharges in the through hole of cross bar, drives cross bar to rotate when clear water is discharged in through hole, and when in drip chamber, clear water reaches a certain amount of, cross bar rotates and stirs clear water, drip chamber is cleaned more thorough.After drainage mechanism discharges clear water, air passes into cross bar, and discharges from through hole, and cross bar rotates, and accelerates air current flow in drip chamber, accelerates drip chamber rate of drying.And when solution is injected drip chamber by liquid storage cylinder, pass into air and make cross bar rotate, cross bar agitating solution makes air better mix with solution, increase solution oxygen level, thus the time making the cell survival on cell battery lead plate longer.

As preferably, described wiper mechanism also comprises air heater, and the air outlet of described air heater is communicated with the air inlet of air pump, and described air heater is electrically connected with computing machine.When cleaning, heating arrangement work, air pump passes into hot-air in drip chamber, accelerates drip chamber rate of drying, and when increasing solution oxygen level in drip chamber, heating arrangement does not work, and air pump passes into the air under normal temperature state in drip chamber.

As preferably, described drainage mechanism comprises the drainpipe be arranged on bottom drip chamber, and described drainpipe is communicated with drip chamber, and described drainpipe is provided with drain solenoid valve, and described drain solenoid valve is electrically connected with computing machine.

A kind of phenylthiourea solution concentration detection method of the present invention, comprises the following steps:

S1: pour in liquid storage cylinder corresponding with drip chamber on disk by phenylthiourea solution to be measured, then injects drip chamber by the phenylthiourea solution in liquid storage cylinder;

S2: phenylthiourea solution drips on cell battery lead plate by liquid droping head, micro pump controls dropping liquid amount and the dropping liquid time of liquid droping head, liquid droping head at interval of 2.1 seconds dropping liquids once, each dropping liquid amount is 0.05ml, dropping liquid 260 times altogether, dropping liquid terminates the surplus solution in rear discharge drip chamber, and carries out cleaning to drip chamber and dry up;

S3: the signal that SAW (Surface Acoustic Wave) resonator gathers on cell battery lead plate is sent to converting unit, converting unit output frequency response curve is to computing machine, the frequency response curve up-sampling that computing machine is receiving, obtain 110-120 sampled value, using sampled value as input data S (t), input second-order linear system accidental resonance model $\frac{d^{2}x\left(t\right)}{{\mathrm{dt}}^2}+[{4r}^2+2\mathrm{\ξ}\left(t\right)+b{\mathrm{\ξ}}^2\left(t\right)]\frac{\mathrm{dx}\left(t\right)}{\mathrm{dt}}+{\mathrm{\ω}}^{4}x\left(t\right)=A\cos \left(\mathrm{\Ωt}\right)+\mathrm{cS}\left(t\right)$ In, and second-order linear system accidental resonance model is resonated,

Wherein, x (t) is the displacement of vibration particle, and Ω is angular frequency, r and ω is the attenuation coefficient of setting and the frequency of linear oscillator particle respectively, and c is the signal adjustment coefficient of setting, and b is the quadratic noise ξ of setting ²t the coefficient of (), ξ (t) is three discrimination noises, and { dismutation of noise follows Poisson distribution to ξ (t) ∈ for-a, 0, a}, a > 0, and its probability distribution is p _s(a)=p _s(-a)=q, p _s(0)=1-2q, wherein 0 < q < 0.5;

Noise average and correlativity follow < ξ (t) >=0, < ξ (t) ξ (t+ τ) >=2qa ²e ^{-λ τ},

Wherein λ is correlation ratio, and the flatness of three discriminations noise ξ (t) is

The output signal-to-noise ratio obtaining phenylthiourea solution to be measured is $\mathrm{SNR}=\frac{\mathrm{r\&lambda;\&Omega;}+{2\mathrm{qa}}^2\mathrm{bc}({\mathrm{\&Omega;}}^3-\mathrm{\&Omega;})}{1+{2\mathrm{qa}}^{2}b+5r-a^2};$

S4: the output signal-to-noise ratio SNR obtained is substituted into phenylthiourea solution concentration forecast model: calculate the concentration k of phenylthiourea solution to be measured.

As preferably, described phenylthiourea solution concentration forecast model is obtained by following steps:

N1: the phenylthiourea solution obtaining variable concentrations in advance, pours in each liquid storage cylinder on disk respectively by the phenylthiourea solution of variable concentrations;

N2: the phenylthiourea solution in liquid storage cylinder corresponding with drip chamber on computer controlled rounding dish injects drip chamber, phenylthiourea solution in drip chamber drips on cell battery lead plate by liquid droping head, micro pump controls dropping liquid amount and the dropping liquid time of liquid droping head, liquid droping head at interval of 2.1 seconds dropping liquids once, each dropping liquid amount is 0.05ml, altogether dropping liquid 260 times;

N3: after dropping liquid terminates, phenylthiourea solution remaining in drip chamber is discharged by drainage mechanism, and wiper mechanism carries out cleaning to drip chamber and dries up;

N4: the signal that SAW (Surface Acoustic Wave) resonator gathers on cell battery lead plate is sent to converting unit, converting unit output frequency response curve is to computing machine, the frequency response curve up-sampling that computing machine is receiving, obtain 110-120 sampled value, using sampled value as input data S (t), input second-order linear system accidental resonance model $\frac{d^{2}x\left(t\right)}{{\mathrm{dt}}^2}+[{4r}^2+2\mathrm{\&xi;}\left(t\right)+b{\mathrm{\&xi;}}^2\left(t\right)]\frac{\mathrm{dx}\left(t\right)}{\mathrm{dt}}+{\mathrm{\&omega;}}^{4}x\left(t\right)=A\cos \left(\mathrm{\&Omega;t}\right)+\mathrm{cS}\left(t\right)$ In, and second-order linear system accidental resonance model is resonated, obtain the output signal-to-noise ratio of this phenylthiourea solution;

N5: computer controlled rounding dish turns an angle, next liquid storage cylinder is turned to the position corresponding with drip chamber, phenylthiourea solution in this liquid storage cylinder is injected drip chamber, repeated execution of steps N2 is to step N4, obtain the output signal-to-noise ratio of the phenylthiourea solution in this liquid storage cylinder, then disk turns an angle again, detect the output signal-to-noise ratio of the phenylthiourea solution in next liquid storage cylinder, so circulation is until detect the output signal-to-noise ratio of the phenylthiourea solution on disk in all liquid storage cylinders;

N6: the concentration of the phenylthiourea solution obtained in advance is respectively k ₁, k ₂..., k _n, the output signal-to-noise ratio of its correspondence is respectively SNR ₁, SNR ₂..., SNR _n, computing machine is by point (k ₁, SNR ₁), (k ₂, SNR ₂) ..., (k _n, SNR _n) fit to straight line, the forecast model of phenylthiourea solution is obtained according to the straight line of matching:

Substantial effect of the present invention is: structure is simple, cost is low, after each dropping liquid completes, wiper mechanism can carry out cleaning to drip chamber and dry up, the concentration ensureing next time to inject the phenylthiourea solution of drip chamber can not be subject to impurity interference, and improve accuracy of detection, pick-up unit controls automatic operation by computing machine, detection speed is fast, improves detection efficiency.

Accompanying drawing explanation

Fig. 1 is a kind of structural representation of the present invention;

Fig. 2 is a kind of structural representation of disk of the present invention;

Fig. 3 is that a kind of circuit theory of the present invention connects block diagram;

Fig. 4 is a kind of structural representation of cell battery lead plate of the present invention;

Fig. 5 is a kind of structural representation of cross bar of the present invention;

Fig. 6 is a kind of structural representation of SAW (Surface Acoustic Wave) resonator of the present invention.

In figure: 1, computing machine, 2, cell battery lead plate, 3, SAW (Surface Acoustic Wave) resonator, 4, converting unit, 5, base, 6, standing groove, 7, drip chamber, 8, micro pump, 9, liquid droping head, 10, inlet, 11, disk, 12, liquid storage cylinder, 13, liquid outlet, 14, liquid outlet electromagnetic valve, 15, drive motor, 16, substrate, 17, work drive motor, 18, to electrode, 19, contrast electrode, 20, painting layer, 21, air intake opening, 22, water inlet, 23, air pump, 24, water pump, 25, air inlet electromagnetic valve, 26, entering water electromagnetic valve, 27, cross bar, 28, vertical pole, 29, through hole, 30, air heater, 31, drainpipe, 32, drain solenoid valve, 33, piezoelectric substrate, 34, interdigital transducer, 35, reflecting grating, 36, gain grid, 37, sound absorption part, 38, link, 39, adapter sleeve, 40, retaining valve, 41, first groove, 42, second groove, 43, osculum, 44, positioning pedestal, 45, lifter, 46, voice-output unit.

Embodiment

Below by embodiment, and by reference to the accompanying drawings, technical scheme of the present invention is described in further detail.

Embodiment: a kind of phenylthiourea solution concentration detector of the present embodiment, as Fig. 1, Fig. 2, shown in Fig. 3, comprise computing machine 1, collecting unit and collecting work platform, collecting unit comprises cell battery lead plate 2, SAW (Surface Acoustic Wave) resonator 3 and converting unit 4, collecting work platform comprises base 5, base 5 is provided with standing groove 6, lifter 45 and voice-output unit 46, drip chamber 7 is provided with above standing groove 6, drip chamber 7 is connected with lifter 45 by bindiny mechanism, the micro pump 8 be communicated with drip chamber 7 is provided with bottom drip chamber 7, the liquid droping head 9 be communicated with micro pump 8 is provided with bottom micro pump 8, drip chamber 7 top is provided with inlet 10, wiper mechanism and drainage mechanism is also provided with in drip chamber 7, disk 11 is provided with above drip chamber 7, disk 11 is provided with several liquid storage cylinders 12 around the center of circle, position corresponding with inlet 10 bottom liquid storage cylinder 12 is provided with liquid outlet 13, liquid outlet 13 is provided with liquid outlet electromagnetic valve 14, drip chamber 7 top is provided with the drive motor 15 that driver plate 11 rotates.

Wiper mechanism comprises air intake opening 21, water inlet 22 and the rabbling mechanism be arranged in drip chamber, rabbling mechanism comprises the vertical pole 28 of the cross bar 27 of horizontally disposed hollow and the hollow of longitudinally setting, as shown in Figure 5, the bottom in cross bar 27 stage casing is located at the top of vertical pole 28 by bearing holder (housing, cover), cavity connects in cavity in cross bar 27 and vertical pole 28, the leading flank of cross bar 27 one end and the trailing flank of cross bar 27 other end are provided with several through holes 29, through hole 29 is communicated with cross bar 27 internal cavity, vertical pole 28 is arranged on bottom drip chamber 7, cavity in vertical pole 28 is communicated with water inlet 22 with air intake opening 21 by a connecting line, air intake opening 21 is connected with air pump 23, water inlet 22 is connected with water pump 24, water pump 24 is connected with water tank, air intake opening 21 is provided with air inlet electromagnetic valve 25, water inlet 22 is provided with entering water electromagnetic valve 26.Drainage mechanism comprises the drainpipe 31 be arranged on bottom drip chamber 7, and drainpipe 31 is communicated with drip chamber 7, and drainpipe 31 is provided with drain solenoid valve 32.

SAW (Surface Acoustic Wave) resonator 3 is electrically connected with cell battery lead plate 2 and converting unit 4 respectively, and computing machine 1 is electrically connected with converting unit 4, micro pump 8, drive motor 15, liquid outlet electromagnetic valve 14, air inlet electromagnetic valve 25, entering water electromagnetic valve 26, drain solenoid valve 32, air pump 23, water pump 24, lifter 45 and voice output circuit 46 respectively.Converting unit 4 comprises oscillatory circuit and frequency detector, and SAW (Surface Acoustic Wave) resonator 3 is electrically connected with frequency detector by oscillatory circuit, and frequency detector is electrically connected with computing machine 1.

Standing groove 6 comprises the first groove 41 and the second groove 42, first groove 41 is positioned at below drainpipe 31, second groove 42 is positioned at below liquid droping head 9, positioning pedestal 44 is provided with in second groove 42, positioning pedestal 44 is provided with the locating slot coordinated with cell battery lead plate 2, the sidewall slope of positioning pedestal 44 is arranged, and be all provided with osculum 43 bottom the first groove 41 He bottom the second groove 42, osculum 43 is connected with outside effluent storage device by discharge pipe line.The rotating shaft of drive motor 15 is arranged with adapter sleeve 39, disk 11 and adapter sleeve 39 removably connect, and are convenient to disk and pull down cleaning.Air intake opening 21 is positioned at above water inlet 22.Connecting line is provided with retaining valve 40, prevents liquid backflow.Liquid droping head for controlling drip chamber lifting, thus is adjusted to suitable position by lifter.Voice-output unit is for exporting voice message and testing result.Gap is there is between drip chamber and disk.

As shown in Figure 4, cell battery lead plate 2 comprises substrate 16, substrate 16 is provided with working electrode 17, to electrode 18 and two contrast electrodes 19, working electrode 17 comprises the rounded portions of front end, this rounded portions is made up of foam copper, this foam copper is coated with one deck Gold plated Layer, Gold plated Layer is provided with mouse bacterium shape taste bud taste receptor cells, to the front end of electrode 18 with the front end of contrast electrode 19 is curved is centered around outside rounded portions, working electrode 17 front end, painting layer 20 is scribbled to electrode 18 front end and contrast electrode 19 front end outer side.To electrode 18, the upper surface of contrast electrode 19 and working electrode 17 is all higher than substrate 16 upper surface 0.6 centimetre, substrate 16 is made up of ST cut type quartz, electrode 18 and contrast electrode 19 are made up of tungsten, working electrode 18 is made up of foam copper material, foam copper is equally distributed three-dimensional netted pore structure, it has the reticulate texture of multi-layer stable and connects closely, reticulate texture is not yielding and subside, this makes cell can enter foam copper inside configuration, cell can better be attached on electrode, it is lower that Gold plated Layer compares foam copper toxicity, make cytoactive better with cells contacting, also make detection sensitivity better.There is no voltage difference between two contrast electrodes, be equivalent to the region having a voltage constant between them, whole region as Voltage Reference, the stability that favourable raising detects.

As shown in Figure 6, SAW (Surface Acoustic Wave) resonator 3 comprises piezoelectric substrate 33, interdigital transducer 34, two reflecting gratings 35, is located at four gain grid 36 of reflecting grating 35 both sides and is arranged at two sound absorption parts 37 away from interdigital transducer 34 side of two reflecting gratings 35 respectively, be electrically connected with two links 38 be located on interdigital transducer 34 respectively electrode 18 and working electrode 17, contrast electrode 19 is connected with working electrode 17 by 5V bias direct current voltage.

Obtain the phenylthiourea solution of variable concentrations in advance, poured into respectively by the phenylthiourea solution of variable concentrations in each liquid storage cylinder on disk, be placed in the locating slot of positioning pedestal by cell battery lead plate, now cell battery lead plate is positioned at below liquid droping head.

Start the work of phenylthiourea solution concentration detector, liquid outlet electromagnetic valve on liquid storage cylinder corresponding with drip chamber on computer controlled rounding dish opens (aiming at the inlet on drip chamber with the liquid outlet on the liquid storage cylinder that drip chamber is corresponding), and the phenylthiourea solution in liquid storage cylinder injects drip chamber by inlet.Then computing machine controls micro pump work, and micro pump controls liquid droping head at interval of 2.1 seconds dropping liquids once, and each dropping liquid amount is 0.05ml, altogether dropping liquid 260 times.Phenylthiourea solution in drip chamber drips on cell battery lead plate by liquid droping head, cell battery lead plate produces specificity current-responsive, current-responsive is captured by SAW (Surface Acoustic Wave) resonator and outputs to converting unit in the frequency form, converting unit detects frequency signal, and output frequency response curve is to computing machine.The frequency response curve up-sampling that computing machine is receiving, obtains 120 sampled values, using sampled value as input data S (t), and input second-order linear system accidental resonance model $\frac{d^{2}x\left(t\right)}{{\mathrm{dt}}^2}+[{4r}^2+2\mathrm{\&xi;}\left(t\right)+b{\mathrm{\&xi;}}^2\left(t\right)]\frac{\mathrm{dx}\left(t\right)}{\mathrm{dt}}+{\mathrm{\&omega;}}^{4}x\left(t\right)=A\cos \left(\mathrm{\&Omega;t}\right)+\mathrm{cS}\left(t\right)$ In, and second-order linear system accidental resonance model is resonated, obtain the output signal-to-noise ratio of this phenylthiourea solution $\mathrm{SNR}=\frac{\mathrm{r\&lambda;\&Omega;}+{2\mathrm{qa}}^2\mathrm{bc}({\mathrm{\&Omega;}}^3-\mathrm{\&Omega;})}{1+{2\mathrm{qa}}^{2}b+5r-a^2}.$

After dropping liquid terminates, phenylthiourea solution remaining in drip chamber is discharged by drainage mechanism, and wiper mechanism carries out cleaning to drip chamber and dries up.Then, computing machine rotates clockwise certain angle by drive motor driver plate, next liquid storage cylinder is made to turn to the position corresponding with drip chamber, then the output signal-to-noise ratio that above-mentioned steps detects phenylthiourea solution in this liquid storage cylinder is repeated, then phenylthiourea solution remaining in drip chamber is discharged by drainage mechanism, and wiper mechanism carries out cleaning to drip chamber and dries up.Circulation like this is until detect the output signal-to-noise ratio of the phenylthiourea solution on disk in all liquid storage cylinders, and the concentration of the phenylthiourea solution obtained in advance is respectively k ₁, k ₂..., k _n, the output signal-to-noise ratio of its correspondence is respectively SNR ₁, SNR ₂..., SNR _n, computing machine is by point (k ₁, SNR ₁), (k ₂, SNR ₂) ..., (k _n, SNR _n) fit to straight line, the forecast model of phenylthiourea solution is obtained according to the straight line of matching:

When detecting the phenylthiourea solution to be measured of unknown concentration, as long as phenylthiourea solution to be measured is poured into in liquid storage cylinder corresponding with drip chamber on disk, repeat the output signal-to-noise ratio SNR that above-mentioned steps obtains this phenylthiourea solution, this output signal-to-noise ratio SNR substituted into the forecast model of phenylthiourea solution: thus calculate the concentration k of phenylthiourea solution to be measured.

Wiper mechanism also comprises air heater 30, and the air outlet of air heater 30 is communicated with the air inlet of air pump 23, and air heater 30 is electrically connected with computing machine 1.After each dropping liquid terminates, the surplus solution in drip chamber is first discharged by drainage mechanism, and then drain solenoid valve is closed, and cleans.Close air inlet electromagnetic valve, start water pump, open entering water electromagnetic valve, clear water passes into cross bar from vertical pole, and discharge in the through hole of cross bar, drive cross bar to rotate, when clear water reaches a certain amount of in drip chamber when clear water is discharged in through hole, cross bar rotates and stirs clear water, drip chamber is cleaned more thorough.Then water is discharged by drainage mechanism, switches off the pump and entering water electromagnetic valve, and booster air pump, air heater, unlatching air inlet electromagnetic valve, air pump passes into hot-air and dried up by drip chamber in drip chamber.After drainage mechanism discharges clear water, hot-air passes into cross bar, and discharges from through hole, and cross bar rotates, and accelerates air current flow in drip chamber, accelerates drip chamber rate of drying.

When solution is injected drip chamber by liquid storage cylinder, pass into normal temperature air and cross bar is rotated, cross bar agitating solution makes air better mix with solution, increases solution oxygen level, thus the time making the cell survival on cell battery lead plate longer.

A kind of phenylthiourea solution concentration detection method of the present embodiment, is applicable to above-mentioned phenylthiourea solution concentration detector, comprises the following steps:

S1: cell battery lead plate is put into standing groove, phenylthiourea solution to be measured is poured in liquid storage cylinder corresponding with drip chamber on disk, then liquid droping head is adjusted to assigned address by lifter, then the phenylthiourea solution in liquid storage cylinder is injected drip chamber, after solution in liquid storage cylinder all injects drip chamber, passing into normal temperature air a period of time makes cross bar rotate, cross bar agitating solution makes air better mix with solution, increase solution oxygen level, thus the time making the cell survival on cell battery lead plate longer;

S2: phenylthiourea solution drips on cell battery lead plate by liquid droping head, micro pump controls dropping liquid amount and the dropping liquid time of liquid droping head, liquid droping head at interval of 2.1 seconds dropping liquids once, each dropping liquid amount is 0.05ml, dropping liquid 260 times altogether, after dropping liquid terminates, open drain solenoid valve, surplus solution in drip chamber is discharged, drain solenoid valve is closed after discharge, then water pump is started, open entering water electromagnetic valve and pass into clear water, drip chamber is cleaned, switch off the pump after cleaning and entering water electromagnetic valve, open drain solenoid valve again and discharge water, then booster air pump, air heater, open air inlet electromagnetic valve, pass into hot-air drip chamber is dried up, finally close drain solenoid valve, air pump, air heater and air inlet electromagnetic valve,

S3: the signal that SAW (Surface Acoustic Wave) resonator gathers on cell battery lead plate is sent to converting unit, converting unit output frequency response curve is to computing machine, the frequency response curve up-sampling that computing machine is receiving, obtain 120 sampled values, using sampled value as input data S (t), input second-order linear system accidental resonance model $\frac{d^{2}x\left(t\right)}{{\mathrm{dt}}^2}+[{4r}^2+2\mathrm{\&xi;}\left(t\right)+b{\mathrm{\&xi;}}^2\left(t\right)]\frac{\mathrm{dx}\left(t\right)}{\mathrm{dt}}+{\mathrm{\&omega;}}^{4}x\left(t\right)=A\cos \left(\mathrm{\&Omega;t}\right)+\mathrm{cS}\left(t\right)$ In, and second-order linear system accidental resonance model is resonated,

Wherein, x (t) is the displacement of vibration particle, and Ω is angular frequency, r and ω is the attenuation coefficient of setting and the frequency of linear oscillator particle respectively, and c is the signal adjustment coefficient of setting, and b is the quadratic noise ξ of setting ²t the coefficient of (), ξ (t) is three discrimination noises, and { dismutation of noise follows Poisson distribution to ξ (t) ∈ for-a, 0, a}, a > 0, and its probability distribution is p _s(a)=p _s(-a)=q, p _s(0)=1-2q, wherein 0 < q < 0.5;

Noise average and correlativity follow < ξ (t) >=0, < ξ (t) ξ (t+ τ) >=2qa ²e ^{-λ τ},

Wherein λ is correlation ratio, and the flatness of three discriminations noise ξ (t) is

The output signal-to-noise ratio obtaining phenylthiourea solution to be measured is $\mathrm{SNR}=\frac{\mathrm{r\&lambda;\&Omega;}+{2\mathrm{qa}}^2\mathrm{bc}({\mathrm{\&Omega;}}^3-\mathrm{\&Omega;})}{1+{2\mathrm{qa}}^{2}b+5r-a^2};$

S4: the output signal-to-noise ratio SNR obtained is substituted into phenylthiourea solution concentration forecast model: calculate the concentration k of phenylthiourea solution to be measured.

When model resonates, particle produces resonance in certain position, and this angular frequency Ω, attenuation coefficient r, correlation ratio λ, coefficient b, constant a, q determine, therefore can calculate signal to noise ratio snr.

Phenylthiourea solution concentration forecast model is obtained by following steps:

N1: the phenylthiourea solution obtaining variable concentrations in advance, poured into respectively by the phenylthiourea solution of variable concentrations in each liquid storage cylinder on disk, cell battery lead plate is put into standing groove, and then liquid droping head is adjusted to assigned address by lifter;

N2: the phenylthiourea solution in liquid storage cylinder corresponding with drip chamber on computer controlled rounding dish injects drip chamber, phenylthiourea solution in drip chamber drips on cell battery lead plate by liquid droping head, micro pump controls dropping liquid amount and the dropping liquid time of liquid droping head, liquid droping head at interval of 2.1 seconds dropping liquids once, each dropping liquid amount is 0.05ml, altogether dropping liquid 260 times;

N3: after dropping liquid terminates, phenylthiourea solution remaining in drip chamber is discharged by drainage mechanism, and wiper mechanism carries out cleaning to drip chamber and dries up;

N4: the signal that SAW (Surface Acoustic Wave) resonator gathers on cell battery lead plate is sent to converting unit, converting unit output frequency response curve is to computing machine, the frequency response curve up-sampling that computing machine is receiving, obtain 120 sampled values, using sampled value as input data S (t), input second-order linear system accidental resonance model $\frac{d^{2}x\left(t\right)}{{\mathrm{dt}}^2}+[{4r}^2+2\mathrm{\&xi;}\left(t\right)+b{\mathrm{\&xi;}}^2\left(t\right)]\frac{\mathrm{dx}\left(t\right)}{\mathrm{dt}}+{\mathrm{\&omega;}}^{4}x\left(t\right)=A\cos \left(\mathrm{\&Omega;t}\right)+\mathrm{cS}\left(t\right)$ In, and second-order linear system accidental resonance model is resonated, obtain the output signal-to-noise ratio of this phenylthiourea solution;

N5: computer controlled rounding dish turns an angle, next liquid storage cylinder is turned to the position corresponding with drip chamber, phenylthiourea solution in this liquid storage cylinder is injected drip chamber, repeated execution of steps N2 is to step N4, obtain the output signal-to-noise ratio of the phenylthiourea solution in this liquid storage cylinder, then disk turns an angle again, detect the output signal-to-noise ratio of the phenylthiourea solution in next liquid storage cylinder, so circulation is until detect the output signal-to-noise ratio of the phenylthiourea solution on disk in all liquid storage cylinders;

N6: the concentration of the phenylthiourea solution obtained in advance is respectively k ₁, k ₂..., k _n, the output signal-to-noise ratio of its correspondence is respectively SNR ₁, SNR ₂..., SNR _n, computing machine is by point (k ₁, SNR ₁), (k ₂, SNR ₂) ..., (k _n, SNR _n) fit to straight line, the forecast model of phenylthiourea solution is obtained according to the straight line of matching:

Step N4 Computer is when the frequency response curve up-sampling received, the frequency recorded during front 30 dropping liquids of liquid droping head is not sampled, because remain the solution once detected on the dropping liquid incipient cell battery lead plate detected each liquid storage luminal solution, after front 30 dropping liquids, on cell battery lead plate, the last solution detected is washed, and ensure that accuracy of detection.

Claims (9)

1. a phenylthiourea solution concentration detector, it is characterized in that: comprise computing machine (1), collecting unit and collecting work platform, described collecting unit comprises cell battery lead plate (2), SAW (Surface Acoustic Wave) resonator (3) and converting unit (4), described collecting work platform comprises base (5), described base (5) is provided with standing groove (6) and lifter (45), described standing groove (6) top is provided with drip chamber (7), described drip chamber (7) is connected with lifter (45) by bindiny mechanism, described drip chamber (7) bottom is provided with the micro pump (8) be communicated with drip chamber (7), described micro pump (8) bottom is provided with the liquid droping head (9) be communicated with micro pump, described drip chamber (7) top is provided with inlet (10), described drip chamber is also provided with wiper mechanism and drainage mechanism in (7), described drip chamber (7) top is provided with disk (11), described disk (11) is provided with several liquid storage cylinders (12) around the center of circle, the position corresponding with inlet (10), described liquid storage cylinder (12) bottom is provided with liquid outlet (13), described liquid outlet (13) is provided with liquid outlet electromagnetic valve (14), described drip chamber (7) top is provided with the drive motor (15) that driver plate rotates, described SAW (Surface Acoustic Wave) resonator (3) is electrically connected with cell battery lead plate (2) and converting unit (4) respectively, described computing machine (1) respectively with converting unit (4), micro pump (8), wiper mechanism, drainage mechanism, liquid outlet electromagnetic valve (14), drive motor (15) and lifter (45) electrical connection.

2. a kind of phenylthiourea solution concentration detector according to claim 1, it is characterized in that: described cell battery lead plate (2) comprises substrate (16), described substrate (16) is provided with working electrode (17), to electrode (18) and two contrast electrodes (19), described working electrode (17) comprises the rounded portions of front end, this rounded portions is made up of foam copper, this foam copper is coated with one deck Gold plated Layer, described Gold plated Layer is provided with mouse bacterium shape taste bud taste receptor cells, the front end of the described front end to electrode (18) and contrast electrode (19) is curved to be centered around outside rounded portions, described working electrode (17) front end, painting layer (20) is scribbled to electrode (18) front end and contrast electrode (19) front end outer side.

3. a kind of phenylthiourea solution concentration detector according to claim 1, it is characterized in that: described converting unit (4) comprises oscillatory circuit and frequency detector, described SAW (Surface Acoustic Wave) resonator (3) is electrically connected with frequency detector by oscillatory circuit, and described frequency detector is electrically connected with computing machine (1).

4. a kind of phenylthiourea solution concentration detector according to claim 1 or 2 or 3, it is characterized in that: described wiper mechanism comprises air intake opening (21) and water inlet (22), described drip chamber (7) is communicated with water inlet (22) with air intake opening (21) by a connecting line, described air intake opening (2) is connected with air pump (23), described water inlet (22) is connected with water pump (24), described water pump (24) is connected with water tank, described air intake opening (21) is provided with air inlet electromagnetic valve (25), described water inlet (22) is provided with entering water electromagnetic valve (26), described air pump (23), water pump (24), air inlet electromagnetic valve (25) and entering water electromagnetic valve (26) are electrically connected with computing machine (1) respectively.

5. a kind of phenylthiourea solution concentration detector according to claim 4, it is characterized in that: described wiper mechanism also comprises the rabbling mechanism be arranged in drip chamber (7), described rabbling mechanism comprises the vertical pole (28) of the cross bar (27) of horizontally disposed hollow and the hollow of longitudinally setting, the bottom in described cross bar (27) stage casing is located at the top of vertical pole (28) by bearing holder (housing, cover), cavity connects in cavity in described cross bar (27) and vertical pole (28), the leading flank of described cross bar (27) one end and the trailing flank of cross bar (27) other end are provided with several through holes (29), described through hole (29) is communicated with cross bar (27) internal cavity, described vertical pole (28) is arranged on drip chamber (7) bottom, cavity in described vertical pole (28) is communicated with water inlet (22) with air intake opening (21) by connecting line.

6. a kind of phenylthiourea solution concentration detector according to claim 4, it is characterized in that: described wiper mechanism also comprises air heater (30), the air outlet of described air heater (30) is communicated with the air inlet of air pump (23), and described air heater (30) is electrically connected with computing machine (1).

7. a kind of phenylthiourea solution concentration detector according to claim 1 or 2 or 3, it is characterized in that: described drainage mechanism comprises the drainpipe (31) being arranged on drip chamber (7) bottom, described drainpipe (31) is communicated with drip chamber (7), described drainpipe (31) is provided with drain solenoid valve (32), and described drain solenoid valve (32) is electrically connected with computing machine (1).

8. a phenylthiourea solution concentration detection method, is applicable to a kind of phenylthiourea solution concentration detector in claim 1-7 described in arbitrary claim, it is characterized in that, comprise the following steps:

S1: pour in liquid storage cylinder corresponding with drip chamber on disk by phenylthiourea solution to be measured, then injects drip chamber by the phenylthiourea solution in liquid storage cylinder;

S2: phenylthiourea solution drips on cell battery lead plate by liquid droping head, micro pump controls dropping liquid amount and the dropping liquid time of liquid droping head, liquid droping head at interval of 2.1 seconds dropping liquids once, each dropping liquid amount is 0.05ml, dropping liquid 260 times altogether, dropping liquid terminates the surplus solution in rear discharge drip chamber, and carries out cleaning to drip chamber and dry up;

S3: the signal that SAW (Surface Acoustic Wave) resonator gathers on cell battery lead plate is sent to converting unit, converting unit output frequency response curve is to computing machine, the frequency response curve up-sampling that computing machine is receiving, obtain 110-120 sampled value, using sampled value as input data S (t), input second-order linear system accidental resonance model $\frac{d^{2}x\left(t\right)}{{\mathrm{dt}}^2}+[{4r}^2+2\mathrm{\&xi;}\left(t\right)+b{\mathrm{\&xi;}}^2\left(t\right)]\frac{\mathrm{dx}\left(t\right)}{\mathrm{dt}}+{\mathrm{\&omega;}}^{4}x\left(t\right)=A\cos \left(\mathrm{\&Omega;t}\right)+\mathrm{cS}\left(t\right)$ In, and second-order linear system accidental resonance model is resonated,

Wherein, x (t) is the displacement of vibration particle, and Ω is angular frequency, r and ω is the attenuation coefficient of setting and the frequency of linear oscillator particle respectively, and c is the signal adjustment coefficient of setting, and b is the quadratic noise ξ of setting ²t the coefficient of (), ξ (t) is three discrimination noises, and { dismutation of noise follows Poisson distribution to ξ (t) ∈ for-a, 0, a}, a > 0, and its probability distribution is p _s(a)=p _s(-a)=q, p _s(0)=1-2q, wherein 0 < q < 0.5;

Noise average and correlativity follow < ξ (t) >=0, < ξ (t) ξ (t+ τ) >=2qa ²e ^{-λ τ},

Wherein λ is correlation ratio, and the flatness of three discriminations noise ξ (t) is

The output signal-to-noise ratio obtaining phenylthiourea solution to be measured is $\mathrm{SNR}=\frac{\mathrm{r\&lambda;\&Omega;}+2{\mathrm{qa}}^2\mathrm{bc}({\mathrm{\&Omega;}}^3-\mathrm{\&Omega;})}{1+{2\mathrm{qa}}^{2}b+5r-a^2};$

S4: the output signal-to-noise ratio SNR obtained is substituted into phenylthiourea solution concentration forecast model: calculate the concentration k of phenylthiourea solution to be measured.

9. a kind of phenylthiourea solution concentration detection method according to claim 8, is characterized in that: described phenylthiourea solution concentration forecast model is obtained by following steps:

N1: the phenylthiourea solution obtaining variable concentrations in advance, pours in each liquid storage cylinder on disk respectively by the phenylthiourea solution of variable concentrations;

N2: the phenylthiourea solution in liquid storage cylinder corresponding with drip chamber on computer controlled rounding dish injects drip chamber, phenylthiourea solution in drip chamber drips on cell battery lead plate by liquid droping head, micro pump controls dropping liquid amount and the dropping liquid time of liquid droping head, liquid droping head at interval of 2.1 seconds dropping liquids once, each dropping liquid amount is 0.05ml, altogether dropping liquid 260 times;

N3: after dropping liquid terminates, phenylthiourea solution remaining in drip chamber is discharged by drainage mechanism, and wiper mechanism carries out cleaning to drip chamber and dries up;

N4: the signal that SAW (Surface Acoustic Wave) resonator gathers on cell battery lead plate is sent to converting unit, converting unit output frequency response curve is to computing machine, the frequency response curve up-sampling that computing machine is receiving, obtain 110-120 sampled value, using sampled value as input data S (t), input second-order linear system accidental resonance model $\frac{d^{2}x\left(t\right)}{{\mathrm{dt}}^2}+[{4r}^2+2\mathrm{\&xi;}\left(t\right)+b{\mathrm{\&xi;}}^2\left(t\right)]\frac{\mathrm{dx}\left(t\right)}{\mathrm{dt}}+{\mathrm{\&omega;}}^{4}x\left(t\right)=A\cos \left(\mathrm{\&Omega;t}\right)+\mathrm{cS}\left(t\right)$ In, and second-order linear system accidental resonance model is resonated, obtain the output signal-to-noise ratio of this phenylthiourea solution;

N5: computer controlled rounding dish turns an angle, next liquid storage cylinder is turned to the position corresponding with drip chamber, phenylthiourea solution in this liquid storage cylinder is injected drip chamber, repeated execution of steps N2 is to step N4, obtain the output signal-to-noise ratio of the phenylthiourea solution in this liquid storage cylinder, then disk turns an angle again, detect the output signal-to-noise ratio of the phenylthiourea solution in next liquid storage cylinder, so circulation is until detect the output signal-to-noise ratio of the phenylthiourea solution on disk in all liquid storage cylinders;

N6: the concentration of the phenylthiourea solution obtained in advance is respectively k ₁, k ₂..., k _n, the output signal-to-noise ratio of its correspondence is respectively SNR ₁, SNR ₂..., SNR _n, computing machine is by point (k ₁, SNR ₁), (k ₂, SNR ₂) ..., (k _n, SNR _n) fit to straight line, the forecast model of phenylthiourea solution is obtained according to the straight line of matching: