CN105092666B - Quinine concentration detection apparatus and detection method based on biological tongue - Google Patents
Quinine concentration detection apparatus and detection method based on biological tongue Download PDFInfo
- Publication number
- CN105092666B CN105092666B CN201510067436.7A CN201510067436A CN105092666B CN 105092666 B CN105092666 B CN 105092666B CN 201510067436 A CN201510067436 A CN 201510067436A CN 105092666 B CN105092666 B CN 105092666B
- Authority
- CN
- China
- Prior art keywords
- quinine
- computer
- electrode
- container
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of quinine concentration detection apparatus and detection method based on biological tongue, and the quinine concentration detection apparatus is with calculating mechatronics;Including testing stand, detection box, SAW resonator, electronic counter, oscillator and cell electrode chip on testing stand;Oscillator and SAW resonator form oscillation circuit, and counter is electrically connected with oscillation circuit, and SAW resonator is electrically connected with cell electrode chip, and counter is with calculating mechatronics.The present invention has easy to operate, and detection sensitivity is good, and accuracy of detection is high, and detection speed is fast;Device stability is good, it is reproducible the characteristics of.
Description
Technical field
It is high more particularly, to a kind of detection accuracy the present invention relates to solution concentration detection technical field, fireballing base
In the quinine concentration detection apparatus and detection method of biological tongue.
Background technology
Existing solution concentration detection method has Instrumental Analysis detection method and chemical measure, although Instrumental Analysis detection method
It is easy to operate, but there are accuracy of detection it is low the defects of;There are cumbersome, the deficiencies of poor repeatability for chemical measure.
Chinese patent mandate publication number:CN103528877A authorizes publication date on January 22nd, 2014, discloses a kind of small
The online system of detecting concentration of sugar of type biochemical reactor including 1 touch-screen, is responsible for receiving the setting and operation of Field Force;2
Constant flow pump is responsible for being diluted reaction solution;1 online sugar analyzer is responsible for the on-line checking of sugar concentration;1 offline sugar is dense
Analyzer is spent, is responsible for the offline inspection of sugar concentration;1 flush bonding module BL2100 as the control unit of system, controls constant current
The action of the flow velocity of pump and sugared concentration analyzer gathers the sugared concentration Testing value of online sugar analyzer, collection value is carried out
Line corrects, and obtains accurately sugar concentration Testing value, and joining according to the value of offline sugared concentration analyzer to model in biochemical reactor
Number is modified.The disadvantage of this invention is that the low deficiency with poor repeatability of accuracy of detection.
The content of the invention
The goal of the invention of the present invention is to overcome detection method accuracy of detection of the prior art low and poor repeatability
Deficiency provides a kind of detection accuracy height, fireballing quinine concentration detection apparatus and detection method based on biological tongue.
To achieve these goals, the present invention uses following technical scheme:
A kind of quinine concentration detection apparatus based on biological tongue, the quinine concentration detection apparatus is with calculating mechatronics;
Including testing stand, detection box, SAW resonator, electronic counter, oscillator and cell electrode cores on testing stand
Piece;Oscillator and SAW resonator form oscillation circuit, and counter is electrically connected with oscillation circuit, SAW resonator and
Cell electrode chip is electrically connected, and counter is with calculating mechatronics;
The cell electrode chip include substrate, arranged on upper surface of base plate tungsten to electrode, tungsten reference electrode and work electricity
Pole;The cell electrode chip is lain against in detection box, detection box be equipped with can face working electrode drip quinine solution
Micro pump, detection box lower part is equipped with liquid outlet;The working electrode includes foam layers of copper and the sputtering in foam layers of copper
Layer gold sputters and mouse lingual surface bacterium shape taste bud bitter taste receptors cell is equipped in each mesh of layer gold;
The testing stand is equipped with L-shaped support arm, and support arm is equipped with disk, and disk is equipped with n and fills difference respectively
The transfusion bottle of the quinine solution of concentration;The container being located at below disk is equipped in the middle part of support arm, be equipped in container respectively with water inlet pipe,
The agitating device of air inlet pipe connection, agitating device are equipped with several through holes, and container is equipped with cover board, and lower vessel portion is equipped with out liquid
Pipe, micro pump are connected with container lower end;Support arm is connected by the motor that disc level can be driven to rotate with disk, each
Be equipped with solenoid valve in transfusion bottle lower end, water inlet pipe, outlet tube and air inlet pipe, computer respectively with micro pump, motor and each electricity
Magnet valve is electrically connected.
The preparation process of the cell electrode is as follows:
Sputter, target disc fixed seat, electrode supporting plate are equipped in the sputtering chamber of gold processing successively for sputtering, electrode supporting plate leads to
The horizontal rotating shaft for crossing the first motor is connected with sputtering chamber, and target disc fixed seat is connected by the horizontal rotating shaft of the second motor with sputtering chamber
It connects;Watch window is additionally provided on sputtering chamber;The horizontal rotating shaft of the horizontal rotating shaft of first motor and the second motor is coaxial;
(1-1) by purity >=99.99%, a diameter of 4.5 to 5.0cm, the gold target disk that thickness is 0.5 to 0.7cm is installed to
In target disc fixed seat, by including substrate made of ST cut type quartz, on substrate to electrode, reference electrode and foam copper material
The electrode chip of working electrode made of material is installed on electrode supporting plate, make working electrode towards target disc and expose electrode supporting plate it
Outside;
(1-2) vacuumizes sputtering chamber using ionic pump, and vacuum degree is made to reach 4.2 × 10-5Below Pa;It is filled with to sputtering chamber
The argon gas of purity >=99.99%;
(1-3) makes the power of sputter be gradually reduced by W in staged, and the gold atom on target disc is splashed to working electrode
On;During sputtering, the second motor drives target disc at the uniform velocity to rotate, and the first motor drives electrode chip to be more than the second motor
Rotating speed elder generation rotating Vortex, then reversely rotate, then rotating Vortex again;Obtain the electrode chip after sputtering gold;
Electrode chip after sputtering gold is dried, impregnates, after sterilization processing by (1-4), will use mouse lingual surface bacterium shape taste
Cell suspension made of flower bud bitter taste receptors cell is dripped on working electrode, when culture 2 to 3 is small, obtains cell electrode.
The working electrode of the cell electrode chip of the present invention is uniformly divided using made of foam copper product, foam copper has
The three-dimensional netted pore structure of cloth, the net structure of multi-layer stable are completely embedded, and the satisfactory mechanical property of copper can prevent net structure
Deform, collapse and the fracture on surface may.The diameter of working electrode surface mesh is between 0.15mm-0.35mm, and inside it
Mesh diameter it is less and less, this special structure is conducive to molecule or ion moves freely in mesh so that have electricity
The molecule or ion of activity can enter foam copper inside configuration, increase the contact area of solution and electrode to be measured, improve
The sensitivity of working electrode and selectivity.
The present invention further sputters gold atom on the working electrode (s, so as to add one layer of golden film in net structure, gold
Film plays an important role of insulation foam copper and mouse lingual surface bacterium shape taste bud bitter taste receptors cell, because copper is to mouse lingual surface bacterium shape
Taste bud bitter taste receptors cell plays an important role of to poison, and can reduce the work of mouse lingual surface bacterium shape taste bud bitter taste receptors cell
Property, and gold does not have mouse lingual surface bacterium shape taste bud bitter taste receptors cell toxic action, mouse lingual surface bacterium shape taste bud bitter taste taste
Feel that recipient cell activity is more preferable, add the biocompatibility of working electrode, further improve the sensitivity of detection.
The present invention biological tongue be by cultivating mouse bacterium shape taste bud bitter taste receptors cellularity on the working electrode (s,
Since mouse bacterium shape taste bud bitter taste receptors cell has quinine sensitive response, by mouse lingual surface bacterium shape taste bud bitter taste
In foam copper electrode surface as sense of taste sensing element, it is special that cell is subject to the stimulation of taste material molecule to generate for recipient cell culture
Property response, while release the neurotransmitters chemical substance such as ATP and serotonin, these substances have reproducibility, by foam
Copper electrode aoxidizes to obtain specific current-responsive, and current-responsive is captured by surface acoustic wave series resonator, in the form of frequency
Detection can realize the target of taste material information detection according to the detection frequency of SAW resonator.
Therefore, the present invention has easy to operate, and detection sensitivity is good, and accuracy of detection is high, and detection speed is fast;Device stability
Well, the characteristics of reproducible.
Preferably, the agitating device is the T-shaped stirring pipe in lower vessel portion, column is equipped in the container,
Column top edge is equipped with outwards horizontal-extending flange, and the lower edge of T-shaped stirring pipe is equipped with the inside level being adapted with flange
The annular edge of extension, annular edge upper surface and flange lower surface clearance fit;
Column top is equipped with baffle ring, and baffle ring upper surface is in contact with annular edge lower surface;Column lower part and water inlet pipe, air inlet
Pipe is connected;
Each through hole is respectively positioned at the outer circumferential surface rear portion of T-shaped stirring pipe one end and the outer circumferential surface forepart of the other end.
T-shaped stirring pipe can be stirred while be filled with oxygen into solution, high so as to improve the oxygen concentration in solution
The solution of oxygen content can improve the activity of mouse bacterium shape taste bud taste receptor cells, and cytoactive is good, then the sensitivity detected
Higher.
Preferably, it is equipped with polyurethane foam and sputtering layers of copper on the base lower surface successively from the inside to the outside.Substrate
Layers of copper is sputtered on lower surface has reducing catalysis action, can accelerate cell and release the neurotransmitters such as ATP and serotonin
The reduction reaction speed of substance is learned, specific current-responsive is stronger, the sensitivity higher of detection.
Preferably, SAW resonator include piezoelectric substrate, interdigital transducer, 2 reflecting gratings, arranged on reflecting grating two
4 gain grid of side and two of separate the interdigital transducer one side parts that absorb sound for being respectively arranged at two reflecting gratings;To electrode and
Working electrode is electrically connected respectively with 2 connecting pins on interdigital transducer.
A kind of detection method of the quinine concentration detection apparatus based on biological tongue, includes the following steps:
(5-1) fills the quinine solution that concentration gradually rises successively in n transfusion bottle of disk, and computer is according to concentration liter
High order is each transfusion bottle by 1 to n number consecutivelies, the quinine solution concentration k being stored in computer in 1 to n transfusion bottles1,
k2..., kn, the initial value of transfusion bottle sequence number i is 1, i=1 ..., n;
The solenoid valve of (5-2) computer control outlet tube is closed, and controls disk rotational by motor, it is hanging for i to make number
Bottle is aligned with container, and computer controls the solenoid valve of the transfusion bottle to open, and the quinine solution in transfusion bottle is poured into container;
(5-3) removes cover board from container, and the solenoid valve for the air inlet pipe that computer control is connected with oxygen is opened, stirring
Device is filled with oxygen into solution while stirring, and 3 to the control air inlet pipe of computer after five minutes solenoid valve is closed;
(5-4) computer control micro pump drips the quinine solution of 0.05ml every time T to working electrode, when dripping off
Computer control micro pump stops dropping liquid after 300 to 350 drops;While dropping liquid, the frequency of counter acquisition oscillation circuit is rung
Curve is answered, computer gathers several frequency values on frequency response curve, and each frequency values are formed frequency signal S (t);
Second-order linearity accidental resonance model is preset in (5-5) computer
, S (t) is inputted into second-order linearity accidental resonance model and second-order linearity accidental resonance model is made to resonate;
Wherein, x (t) is the displacement for vibrating particle, and Ω is angular frequency, and r and ω are the attenuation coefficient of setting respectively and linear
The frequency of particle is vibrated, c is the signal adjustment coefficient of setting, and b is the quadratic noise ξ of setting2(t) coefficient, ξ (t) are three discriminations
Noise, ξ (t) ∈ {-a, 0, a }, a > 0, the dismutation of noise follow Poisson distribution, probability distribution Ps(a)=Ps(-a)
=q, Ps(0)=1-2q, wherein 0 < q < 0.5;
Noise average is followed with correlation<ξ(t)>=0,<ξ(t)ξ(t+τ)>=2qa2e-λτ;
Wherein λ is correlation ratio, and the glacing flatness of three discrimination noise ξ (t) is
When model resonates, particle generates resonance in some position, this angular frequency Ω, attenuation coefficient r, correlation ratio λ, is
Number b, constant a, q have determined;
Computer utilizes formulaIt calculates and obtains output signal-to-noise ratio SNRi;It calculates
Machine number of queries is the concentration k of the quinine solution of ii, and by kiAnd SNRiIt stores in computer;
(5-6) makes i values increase by 1, the solenoid valve of computer control outlet tube is opened, extra solution from container as i < n
Middle outflow;To cover the opening of upper vessel portion with cover board, the solenoid valve of computer control outlet tube is closed, and by the electricity of water inlet pipe
Magnet valve is opened, and distilled water is filled with into container and cleaning is stirred to container within 1 to 2 minute;The solenoid valve of outlet tube is opened, it will
Distilled water is released;By the solenoid valve of water inlet pipe and outlet tube close, open the solenoid valve of air inlet pipe, to container be filled with 100 to
110 DEG C of dry airs dry container 1-2 minutes;The solenoid valve of computer control air inlet pipe is closed, and is transferred to step (5-2);Work as i
>=n is transferred to step (5-7);
(5-7) computer reads the quinine solution concentration k of storage1, k2..., kn;Computer utilizes point (k1, SNR1),
(k2, SNR2) ..., (kn, SNRn) fitting be in line, quinine concentration prediction model is drawn according to the straight line of fitting:
(5-8) is by micro pump and fills the reservoir of quinine solution W to be detected, and (5-2) detection is utilized to obtain frequency
Signal S (t)w, by S (t)wThe second-order linearity accidental resonance model of input step (5-5), is total to second-order linearity accidental resonance model
It shakes;
And utilize formulaIt calculates and obtains output noise corresponding with quinine solution
Compare SNRw;
(5-9) is by SNRwIt substitutes intoIn, the concentration k of quinine solution W is calculatedw。
Preferably, the T is 1 to 1.2 second.
Preferably, the n is 8 to 15.
Preferably, step (5-4) Computer gathers 95 to 100 frequencies being spacedly distributed on frequency response curve
Rate value.
The present invention is the quinine solution by detecting known concentration, obtains quinine concentration prediction model, and with quinine concentration
Prediction model detects the concentration of the quinine solution of unknown concentration;Stability, the good reliability of detection, high sensitivity, detection speed
Degree is fast.
Therefore, the present invention has the advantages that:
(1) easy to operate, detection sensitivity is good, and accuracy of detection is high, and detection speed is fast;
(2) device stability is good, reproducible.
Description of the drawings
Fig. 1 is a kind of functional block diagram of the present invention;
Fig. 2 is a kind of structure diagram of the detection box of the present invention;
Fig. 3 is a kind of structure diagram of the SAW resonator of the present invention;
Fig. 4 is a kind of structure diagram of the cell electrode chip of the present invention;
Fig. 5 is a kind of flow chart of the embodiment of the present invention;
Fig. 6 is a kind of structure diagram of the present invention;
Fig. 7 is a kind of structure diagram of T-shaped stirring pipe of the invention, column and baffle ring.
In figure:SAW resonator 1, electronic counter 2, oscillator 3, cell electrode chip 4, computer 5, substrate 6,
To electrode 7, reference electrode 8, working electrode 9, detection box 10, micro pump 11, liquid outlet 12, supporting rack 13, container 14, testing stand
15th, support arm 16, disk 17, transfusion bottle 18, agitating device 19, through hole 20, piezoelectric substrate 21, interdigital transducer 22, reflecting grating 23,
It is gain grid 24, sound absorption part 25, connecting pin 26, painting layer 27, outlet tube 28, motor 29, solenoid valve 30, T-shaped stirring pipe 31, perpendicular
Cylinder 32, flange 33, annular edge 34, baffle ring 35, cover board 36.
Specific embodiment
The present invention will be further described with reference to the accompanying drawings and detailed description.
Embodiment as shown in Figure 1 is a kind of quinine concentration detection apparatus based on biological tongue, quinine concentration detection apparatus
With calculating mechatronics;Including testing stand, detection box, SAW resonator 1, electronic counter 2 on testing stand shake
Swing device 3 and cell electrode chip 4;Oscillator and SAW resonator form oscillation circuit, and counter is electrically connected with oscillation circuit
It connects, SAW resonator is electrically connected with cell electrode chip, and counter is electrically connected with computer 5;
As shown in figure 4, cell electrode chip includes substrate 6, the tungsten pair arranged on upper surface of base plate made of ST cut type quartz
Electrode 7, tungsten reference electrode 8 and working electrode 9 and painting layer 27;Reference electrode passes through 5V bias direct currents voltage and working electrode
Connection.1 centimetre of upper surface of base plate is above to electrode, reference electrode and working electrode upper surface.
As shown in Fig. 2, cell electrode chip is lain against in detection box, detection box 10 is equipped with can face working electrode
The micro pump 11 of quinine of dripping solution, detection box lower part are equipped with liquid outlet 12, detect in box and be additionally provided with supporting rack 13;Working electrode
Sputtering layers of copper including foam layers of copper and in foam layers of copper sputters and mouse lingual surface bacterium shape is equipped in each mesh of layers of copper
Taste bud bitter taste receptors cell;
As shown in Figure 6, Figure 7, testing stand 15 is equipped with L-shaped support arm 16, and support arm is equipped with disk 17, and disk is equipped with
The transfusion bottle 18 of the 15 quinine solution for filling various concentration respectively;The container 14 being located at below disk is equipped in the middle part of support arm, is held
The agitating device 19 being connected respectively with water inlet pipe, air inlet pipe is equipped in device, agitating device is equipped with 35 through holes 20;It is set on container
There is cover board 36, lower vessel portion is equipped with outlet tube 28, and micro pump is connected with container lower end;Support arm is by that can drive disc level
The motor 29 of rotation is connected with disk, and solenoid valve 30 is equipped in each transfusion bottle lower end, water inlet pipe, outlet tube and air inlet pipe,
As shown in Figure 1, computer is electrically connected respectively with micro pump, motor and each solenoid valve.
As shown in figure 3, SAW resonator includes 22,2 piezoelectric substrate 21, interdigital transducer reflecting gratings 23, is arranged on
4 gain grid 24 of reflecting grating both sides and two of separate the interdigital transducer one side parts that absorb sound for being respectively arranged at two reflecting gratings
25;Electrode and working electrode are electrically connected respectively with 2 connecting pins 26 on interdigital transducer.Reference electrode is inclined by 5V
DC voltage is put to be connected with working electrode.
As shown in fig. 7, agitating device is the T-shaped stirring pipe 31 in lower vessel portion, column 32 is equipped in the container,
Column top edge is equipped with outwards horizontal-extending flange 33, and the lower edge of T-shaped stirring pipe is equipped with the inside water being adapted with flange
The annular edge 34 of flat extension, annular edge upper surface and flange lower surface clearance fit;Column top is equipped with baffle ring 35, baffle ring upper table
Face is in contact with annular edge lower surface;Column lower part is connected with water inlet pipe, air inlet pipe;Each through hole is located at T-shaped and stirs respectively
The outer circumferential surface rear portion of pipe one end and the outer circumferential surface forepart of the other end.It is equipped with polyurethane foam on base lower surface successively from the inside to the outside
Layer and sputtering layers of copper.
As shown in figure 5, a kind of detection method of the quinine concentration detection apparatus based on biological tongue, includes the following steps:
Step 100, numbered for transfusion bottle
Fill the quinine solution that concentration gradually rises successively in 15 transfusion bottles of disk, computer is raised according to concentration
Order is each transfusion bottle by 1 to 15 number consecutively, is stored in computer in 1 to 15 transfusion bottle being calculated according to allotment ratio
Quinine solution concentration k1, k2..., k15, the initial value of transfusion bottle sequence number i is 1, i=1 ..., 15;
Step 200, the solution numbered in the transfusion bottle for being i is poured into container
Cover board to be removed from container, the solenoid valve of computer control outlet tube is closed, and disk rotational is controlled by motor,
The transfusion bottle that number is made to be i is aligned with container, and computer controls the solenoid valve of the transfusion bottle to open, and the quinine solution in transfusion bottle is poured into
In container;
Step 300, the solution in container is stirred and leads to oxygen
The solenoid valve for the air inlet pipe that computer control is connected with oxygen is opened, and agitating device is filled with while stirring into solution
The solenoid valve closing of oxygen, after five minutes computer control air inlet pipe;
Step 400, it is detected
Computer control micro pump drips the quinine solution of 0.05ml every time T to working electrode, after 300 drop is dripped off
Computer control micro pump stops dropping liquid;While dropping liquid, counter gathers the frequency response curve of oscillation circuit, computer
Several frequency values are gathered on frequency response curve, each frequency values are formed into frequency signal S (t);
Step 500, data processing
Second-order linearity accidental resonance model is preset in computer,
S (t) is inputted into second-order linearity accidental resonance model and second-order linearity accidental resonance model is made to resonate;
Wherein, x (t) is the displacement for vibrating particle, and Ω is angular frequency, and r and ω are the attenuation coefficient of setting respectively and linear
The frequency of particle is vibrated, c is the signal adjustment coefficient of setting, and b is the quadratic noise ξ of setting2(t) coefficient, ξ (t) are three discriminations
Noise, ξ (t) ∈ {-a, 0, a }, a > 0, the dismutation of noise follow Poisson distribution, probability distribution Ps(a)=Ps(-a)
=q, Ps(0)=1-2q, wherein 0 < q < 0.5;
Noise average is followed with correlation<ξ(t)>=0,<ξ(t)ξ(t+τ)>=2qa2e-λτ;
Wherein λ is correlation ratio, and the glacing flatness of three discrimination noise ξ (t) is
Computer utilizes formulaIt calculates and obtains output signal-to-noise ratio SNRi;Computer
Number of queries is the concentration k of the quinine solution of ii, and by kiAnd SNRiIt stores in computer;
Step 600, cycle detection
As i < 15, i values is made to increase by 1, the solenoid valve of computer control outlet tube is opened, and is flowed in extra solution from container
Go out;To cover the opening of upper vessel portion with cover board, the solenoid valve of computer control outlet tube is closed, and by the solenoid valve of water inlet pipe
It opens, distilled water is filled with into container and cleaning is stirred to container within 1 to 2 minute;The solenoid valve of outlet tube is opened, will be distilled
Water is released;The solenoid valve of water inlet pipe and outlet tube is closed, the solenoid valve of air inlet pipe is opened, 110 DEG C of dryings is filled with to container
Air dries container 2 minutes;The solenoid valve of computer control air inlet pipe is closed, and is transferred to step 200;When i >=15, step is transferred to
700;
Step 700, quinine concentration prediction model is drawn
Computer reads the quinine solution concentration k of storage1, k2..., k15;Computer utilizes point (k1, SNR1), (k2,
SNR2) ..., (k15, SNR15) fitting be in line, quinine concentration prediction model is drawn according to the straight line of fitting:
Step 800, the quinine solution W of unknown concentration is detected
Frequency signal is obtained by micro pump and the reservoir for filling quinine solution W to be detected, and using step 200 detection
S(t)w, by S (t)wThe second-order linearity accidental resonance model of input step 500 makes second-order linearity accidental resonance model resonate;
And utilize formulaIt calculates and obtains output noise corresponding with quinine solution
Compare SNRw;
Step 900, the concentration of quinine solution W is obtained
By SNRwIt substitutes intoIn, the concentration k of quinine solution W is calculatedw。
In the present embodiment, T is 1 second, and step 500 Computer gathers 100 to be spacedly distributed on frequency response curve
A frequency values.
It is to be understood that the present embodiment is only illustrative of the invention and is not intended to limit the scope of the invention.In addition, it should also be understood that,
After having read the content of the invention lectured, those skilled in the art can make various modifications or changes to the present invention, these etc.
Valency form is also fallen within the scope of the appended claims of the present application.
Claims (8)
1. a kind of quinine concentration detection apparatus based on biological tongue, the quinine concentration detection apparatus is with calculating mechatronics;Its
It is characterized in, including testing stand, detection box, SAW resonator (1), electronic counter (2), oscillator on testing stand
(3) and cell electrode chip (4);Oscillator and SAW resonator form oscillation circuit, and counter is electrically connected with oscillation circuit
It connects, SAW resonator is electrically connected with cell electrode chip, and counter is electrically connected with computer (5);
The cell electrode chip include substrate (6), arranged on upper surface of base plate tungsten to electrode (7), tungsten reference electrode (8) and work
Make electrode (9);The cell electrode chip is lain against in detection box, and detection box (10) is equipped with can face working electrode drop
The micro pump (11) of lower quinine solution, detection box lower part are equipped with liquid outlet (12);The working electrode includes foam layers of copper and sets
In the sputtering layer gold in foam layers of copper, sputter and mouse lingual surface bacterium shape taste bud bitter taste receptors are equipped in each mesh of layer gold
Cell;
The testing stand (15) is equipped with L-shaped support arm (16), and support arm is equipped with disk (17), and disk is equipped with n respectively
Fill the transfusion bottle (18) of the quinine solution of various concentration;The container (14) being located at below disk is equipped in the middle part of support arm, in container
Equipped with the agitating device (19) being connected respectively with water inlet pipe, air inlet pipe, agitating device is equipped with several through holes (20), on container
Equipped with cover board (36), lower vessel portion is equipped with outlet tube (28), and micro pump is connected with container lower end;Support arm is by that can drive circle
The motor (29) that disk rotates horizontally is connected with disk, and electricity is equipped in each transfusion bottle lower end, water inlet pipe, outlet tube and air inlet pipe
Magnet valve (30), computer are electrically connected respectively with micro pump, motor and each solenoid valve;
The preparation process of the cell electrode is as follows:
Sputter, target disc fixed seat, electrode supporting plate are equipped in the sputtering chamber of gold processing successively for sputtering, electrode supporting plate passes through the
The horizontal rotating shaft of one motor is connected with sputtering chamber, and target disc fixed seat is connected by the horizontal rotating shaft of the second motor with sputtering chamber;It splashes
It penetrates and watch window is additionally provided on room;The horizontal rotating shaft of the horizontal rotating shaft of first motor and the second motor is coaxial;
(1-1) by purity >=99.99%, a diameter of 4.5 to 5.0cm, the gold target disk that thickness is 0.5 to 0.7cm is installed to target disc
In fixed seat, by including substrate made of ST cut type quartz, on substrate to electrode, reference electrode and foam copper product system
Into the electrode chip of working electrode be installed on electrode supporting plate, make working electrode towards target disc and expose outside electrode supporting plate;
(1-2) vacuumizes sputtering chamber using ionic pump, and vacuum degree is made to reach 4.2 × 10-5Below Pa;Purity is filled with to sputtering chamber
>=99.99% argon gas;
(1-3) makes the power of sputter be gradually reduced by W in staged, and the gold atom on target disc is splashed on working electrode;
During sputtering, the second motor drives target disc at the uniform velocity to rotate, and the first motor drives electrode chip to be more than the second motor
Rotating speed elder generation rotating Vortex, then reversely rotate, then rotating Vortex again;Obtain the electrode chip after sputtering gold;
Electrode chip after sputtering gold is dried, impregnates, after sterilization processing by (1-4), and mouse lingual surface bacterium shape taste bud will be used bitter
Cell suspension made of taste taste receptor cells is dripped on working electrode, when culture 2 to 3 is small, obtains cell electrode.
2. the quinine concentration detection apparatus according to claim 1 based on biological tongue, it is characterized in that, the agitating device is
T-shaped stirring pipe (31) in lower vessel portion, the container is interior to be equipped with column (32), and column top edge prolongs equipped with outside level
The flange (33) stretched, the lower edge of T-shaped stirring pipe are equipped with the inside horizontal-extending annular edge (34) being adapted with flange, annular
Side upper surface and flange lower surface clearance fit;
Column top is equipped with baffle ring (35), and baffle ring upper surface is in contact with annular edge lower surface;Column lower part and water inlet pipe, air inlet
Pipe is connected;
Each through hole is respectively positioned at the outer circumferential surface rear portion of T-shaped stirring pipe one end and the outer circumferential surface forepart of the other end.
3. the quinine concentration detection apparatus according to claim 1 based on biological tongue, it is characterized in that, the base lower surface
On from the inside to the outside successively be equipped with polyurethane foam and sputtering layers of copper.
4. the quinine concentration detection apparatus based on biological tongue according to claim 1 or 2 or 3, it is characterized in that, surface acoustic wave
Resonator include piezoelectric substrate, interdigital transducer, 2 reflecting gratings, arranged on reflecting grating both sides 4 gain grid and be respectively arranged at
Two sound absorption parts of the separate interdigital transducer one side of two reflecting gratings;To electrode and working electrode respectively with arranged on interdigital transducing
2 connecting pins electrical connection on device.
5. a kind of detection method suitable for the quinine concentration detection apparatus described in claim 1 based on biological tongue, feature
It is to include the following steps:
(5-1) fills the quinine solution that concentration gradually rises successively in n transfusion bottle of disk, and computer is raised according to concentration
Order is each transfusion bottle by 1 to n number consecutivelies, the quinine solution concentration k being stored in computer in 1 to n transfusion bottles1, k2...,
kn, the initial value of transfusion bottle sequence number i is 1, i=1 ..., n;
(5-2) removes cover board from container, and the solenoid valve of computer control outlet tube is closed, and by motor disk is controlled to turn
Dynamic, the transfusion bottle that number is made to be i is aligned with container, and computer controls the solenoid valve of the transfusion bottle to open, the quinine solution quilt in transfusion bottle
It pours into container;
The solenoid valve for the air inlet pipe that the control of (5-3) computer is connected with oxygen is opened, and agitating device fills while stirring into solution
Enter oxygen, 3 to the control air inlet pipe of computer after five minutes solenoid valve is closed;
(5-4) computer control micro pump drips the quinine solution of 0.05ml every time T to working electrode, when drip off 300 to
Computer control micro pump stops dropping liquid after 350 drops;While dropping liquid, the frequency response of counter acquisition oscillation circuit is bent
Line, computer gather several frequency values on frequency response curve, and each frequency values are formed frequency signal S (t);
Second-order linearity accidental resonance model is preset in (5-5) computer
S (t) is inputted into second-order linearity accidental resonance model and second-order linearity accidental resonance model is made to resonate;
Wherein, x (t) is the displacement for vibrating particle, and Ω is angular frequency, and r and ω are attenuation coefficient and the linear oscillator of setting respectively
The frequency of particle, c are the signal adjustment coefficients of setting, and b is the quadratic noise ξ of setting2(t) coefficient, ξ (t) are three discrimination noises,
ξ (t) ∈ {-a, 0, a }, a > 0, the dismutation of noise follow Poisson distribution, probability distribution ps(a)=ps(- a)=q, ps
(0)=1-2q, wherein 0 < q < 0.5;
Noise average is followed with correlation<ξ(t)>=0,<ξ(t)ξ(t+τ)>=2qa2e-λτ;
Wherein λ is correlation ratio, and the glacing flatness of three discrimination noise ξ (t) is
When model resonates, particle some position generate resonance, this angular frequency Ω, attenuation coefficient r, correlation ratio λ, coefficient b,
Constant a, q have determined;
Computer utilizes the calculation formula of Signal to Noise Ratio (SNR)It calculates and obtains output signal-to-noise ratio
SNRi;The concentration k for the quinine solution that computer inquery number is ii, and by kiAnd SNRiIt stores in computer;
(5-6) makes i values increase by 1, the solenoid valve of computer control outlet tube is opened, and is flowed in extra solution from container as i < n
Go out;To cover the opening of upper vessel portion with cover board, the solenoid valve of computer control outlet tube is closed, and by the solenoid valve of water inlet pipe
It opens, distilled water is filled with into container and cleaning is stirred to container within 1 to 2 minute;The solenoid valve of outlet tube is opened, will be distilled
Water is released;The solenoid valve of water inlet pipe and outlet tube is closed, opens the solenoid valve of air inlet pipe, temperature is filled with as 100 to container
Container is dried 1-2 minutes to 110 DEG C of dry airs;The solenoid valve of computer control air inlet pipe is closed, and is transferred to step (5-2);
As i >=n, step (5-7) is transferred to;
(5-7) computer reads the quinine solution concentration k of storage1, k2..., kn;Computer utilizes point (k1, SNR1), (k2,
SNR2) ..., (kn, SNRn) fitting be in line, quinine concentration prediction model is drawn according to the straight line of fitting:
(5-8) is by micro pump and fills the reservoir of quinine solution W to be detected, and (5-2) detection is utilized to obtain frequency signal S
(t)w, by S (t)wThe second-order linearity accidental resonance model of input step (5-5) makes second-order linearity accidental resonance model resonate;
And utilize formulaIt calculates and obtains output signal-to-noise ratio corresponding with quinine solution
SNRw;
(5-9) is by SNRwIt substitutes intoIn, the concentration k of quinine solution W is calculatedw。
6. the detection method of the quinine concentration detection apparatus according to claim 5 based on biological tongue, it is characterized in that, it is described
T is 1 to 1.2 second.
7. the detection method of the quinine concentration detection apparatus according to claim 5 based on biological tongue, it is characterized in that, it is described
N is 8 to 15.
8. the detection method of the quinine concentration detection apparatus based on biological tongue according to claim 5 or 6 or 7, feature
It is that step (5-4) Computer gathers 95 to 100 frequency values being spacedly distributed on frequency response curve.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510067436.7A CN105092666B (en) | 2015-02-09 | 2015-02-09 | Quinine concentration detection apparatus and detection method based on biological tongue |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510067436.7A CN105092666B (en) | 2015-02-09 | 2015-02-09 | Quinine concentration detection apparatus and detection method based on biological tongue |
Publications (2)
Publication Number | Publication Date |
---|---|
CN105092666A CN105092666A (en) | 2015-11-25 |
CN105092666B true CN105092666B (en) | 2018-05-22 |
Family
ID=54573577
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510067436.7A Expired - Fee Related CN105092666B (en) | 2015-02-09 | 2015-02-09 | Quinine concentration detection apparatus and detection method based on biological tongue |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN105092666B (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110320268B (en) * | 2019-08-15 | 2021-10-29 | 杭州市中医院 | Method for characterizing taste substances in solution by taste detection system |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103149135A (en) * | 2013-03-07 | 2013-06-12 | 浙江工商大学 | Cell suspension concentration sensor |
CN103412004A (en) * | 2013-08-22 | 2013-11-27 | 浙江工商大学 | Method for detecting storage time of citrus sinensis |
CN103424525A (en) * | 2013-03-18 | 2013-12-04 | 浙江工商大学 | Milk quality detection equipment and method |
CN104087778A (en) * | 2013-12-19 | 2014-10-08 | 浙江工商大学 | Preparation method of foamy copper for detection of sucrose concentration |
CN104089996A (en) * | 2014-01-17 | 2014-10-08 | 浙江工商大学 | Taste cell sensor using surface acoustic wave resonator and preparation method thereof |
CN204044164U (en) * | 2014-06-23 | 2014-12-24 | 珠海森龙生物科技有限公司 | Mini-clinical biochemical analyzer |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040191918A1 (en) * | 2003-03-28 | 2004-09-30 | Sandrine Isz | Evaluation of bitterness of active drugs |
-
2015
- 2015-02-09 CN CN201510067436.7A patent/CN105092666B/en not_active Expired - Fee Related
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103149135A (en) * | 2013-03-07 | 2013-06-12 | 浙江工商大学 | Cell suspension concentration sensor |
CN103424525A (en) * | 2013-03-18 | 2013-12-04 | 浙江工商大学 | Milk quality detection equipment and method |
CN103412004A (en) * | 2013-08-22 | 2013-11-27 | 浙江工商大学 | Method for detecting storage time of citrus sinensis |
CN104087778A (en) * | 2013-12-19 | 2014-10-08 | 浙江工商大学 | Preparation method of foamy copper for detection of sucrose concentration |
CN104089996A (en) * | 2014-01-17 | 2014-10-08 | 浙江工商大学 | Taste cell sensor using surface acoustic wave resonator and preparation method thereof |
CN204044164U (en) * | 2014-06-23 | 2014-12-24 | 珠海森龙生物科技有限公司 | Mini-clinical biochemical analyzer |
Also Published As
Publication number | Publication date |
---|---|
CN105092666A (en) | 2015-11-25 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN105021665B (en) | Phenylthiourea concentration detection apparatus and detection method based on biological tongue | |
CN105092666B (en) | Quinine concentration detection apparatus and detection method based on biological tongue | |
CN105092675B (en) | citric acid concentration detection device and detection method | |
CN105092663B (en) | Propylthiouracil (PTU) concentration detection apparatus and detection method based on biological tongue | |
CN107941925A (en) | A kind of liquid phase adsorption assay method in real time in situ | |
CN101358941A (en) | Double-face nanometer band electrode array integration sensor capable of being cut and method for manufacturing same | |
CN105021669B (en) | Citric acid concentration detection device and detection method based on biological tongue | |
US20080121023A1 (en) | Masticatory Efficiency Measuring Apparatus and Masticatory Efficiency Measuring Method Using the Same | |
CN105021668B (en) | Cycloheximide concentration detection apparatus and detection method based on biological tongue | |
CN109883877A (en) | Quartz crystal microbalance and humidity sensor based on metal-organic framework material | |
CN105004767B (en) | A kind of phenylthiourea solution concentration detector and method | |
CN105223313B (en) | Detection apparatus and detection method for hydrogen cyanide in research lab | |
CN108918353B (en) | Method for qualitatively detecting particulate matter in atmospheric environment | |
KR102248211B1 (en) | Fermented wine alcohol concentration measurement device using ultra sonic wave | |
KR100949311B1 (en) | Sensor for measuring concentration of microbes | |
CN105021666B (en) | A kind of cycloheximide solution concentration detector and method | |
CN107271315B (en) | A kind of dressing penetrates the ability test method of vapor | |
CN105092664B (en) | A kind of citric acid solution concentration detection apparatus and method | |
JP2000131122A (en) | Manufacture of sensitive film for chemical sensor and chemical sensor probe | |
CN105092665B (en) | A kind of propylthiouracil (PTU) solution concentration detector and method | |
CN206161583U (en) | Circulation formula measuring cell of ability online cleaning pHORP electrode | |
CN207472865U (en) | Multi-example rotates the osmotic pressure detector of sample introduction | |
CN217180458U (en) | Moisture tester | |
CN105116025B (en) | A kind of quinine solution concentration detector and method | |
CN118258724A (en) | Vibration type on-line viscosity measuring device and application thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180522 Termination date: 20190209 |
|
CF01 | Termination of patent right due to non-payment of annual fee |