CN104987407B - 一种镉‑IgG螯合物及其制备方法和应用 - Google Patents
一种镉‑IgG螯合物及其制备方法和应用 Download PDFInfo
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/96—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood or serum control standard
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
1:5 | 1:10 | 1:20 | 1:40 | 1:80 | |
1h | 0.281 | 0.168 | 0.081 | 0.114 | 0.469 |
2h | 0.250 | 0.115 | 0.050 | 0.183 | 0.438 |
3h | 0.225 | 0.106 | 0.100 | 0.196 | 0.441 |
编号 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
OD405 | 0.453 | 0.298 | 0.500 | 0.466 | 0.509 | 0.298 | 0.539 | 0.437 | 0.548 | 0.260 |
编号 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 |
OD405 | 0.626 | 0.468 | 0.561 | 0.481 | 0.327 | 0.459 | 0.630 | 0.591 | 0.475 | 0.526 |
编号 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 |
OD405 | 0.572 | 0.419 | 0.550 | 0.461 | 0.649 | 0.226 | 0.547 | 0.248 | 0.329 | 0.449 |
编号 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 |
OD405 | 0.527 | 0.459 | 0.361 | 0.483 | 0.503 | 0.658 | 0.598 | 0.279 | 0.598 | 0.410 |
编号 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 |
OD405 | 0.486 | 0.488 | 0.539 | 0.434 | 0.493 | 0.403 | 0.570 | 0.587 | 0.497 | 0.224 |
编号 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 |
OD405 | 0.641 | 0.424 | 0.301 | 0.451 | 0.409 | 0.531 | 0.403 | 0.586 | 0.488 | 0.346 |
编号 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 |
OD405 | 0.645 | 0.531 | 0.217 | 0.414 | 0.538 | 0.447 | 0.633 | 0.269 | 0.553 | 0.218 |
编号 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 |
OD405 | 0.587 | 0.268 | 0.418 | 0.376 | 0.469 | 0.502 | 0.447 | 0.569 | 0.291 | 0.628 |
编号 | 81 | 82 | 83 | 84 | 85 | 86 | 87 | 88 | 89 | 90 |
OD405 | 0.418 | 0.528 | 0.421 | 0.518 | 0.228 | 0.321 | 0.269 | 0.541 | 0.290 | 0.529 |
编号 | 91 | 92 | 93 | 94 | 95 | 96 | 97 | 98 | 99 | 100 |
OD405 | 0.610 | 0.596 | 0.483 | 0.553 | 0.403 | 0.525 | 0.223 | 0.544 | 0.214 | 0.568 |
编号 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
μg/L | 0.342 | 0.237 | 0.356 | 0.253 | 0.153 | 0.295 | 0.138 | 0.440 | 0.339 | 0.117 |
编号 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 |
μg/L | 0.108 | 0.222 | 0.222 | 0.366 | 0.154 | 0.099 | 0.128 | 0.253 | 0.123 | 0.386 |
编号 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 |
μg/L | 0.420 | 0.159 | 0.162 | 0.254 | 0.294 | 0.390 | 0.213 | 0.282 | 0.279 | 0.403 |
编号 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 |
μg/L | 0.304 | 0.239 | 0.246 | 0.090 | 0.291 | 0.434 | 0.287 | 0.201 | 0.207 | 0.218 |
编号 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 |
μg/L | 0.357 | 0.324 | 0.379 | 0.327 | 0.090 | 0.279 | 0.236 | 0.284 | 0.349 | 0.424 |
编号 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 |
μg/L | 0.190 | 0.241 | 0.201 | 0.213 | 0.143 | 0.405 | 0.347 | 0.154 | 0.311 | 0.095 |
编号 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 |
μg/L | 0.268 | 0.248 | 0.262 | 0.369 | 0.411 | 0.306 | 0.330 | 0.278 | 0.274 | 0.290 |
编号 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 |
μg/L | 0.386 | 0.342 | 0.327 | 0.371 | 0.150 | 0.268 | 0.249 | 0.415 | 0.273 | 0.096 |
编号 | 81 | 82 | 83 | 84 | 85 | 86 | 87 | 88 | 89 | 90 |
μg/L | 0.167 | 0.135 | 0.385 | 0.354 | 0.317 | 0.424 | 0.229 | 0.294 | 0.215 | 0.174 |
编号 | 91 | 92 | 93 | 94 | 95 | 96 | 97 | 98 | 99 | 100 |
μg/L | 0.139 | 0.388 | 0.352 | 0.156 | 0.230 | 0.090 | 0.281 | 0.245 | 0.254 | 0.421 |
编号 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
μg/L | 0.350 | 0.335 | 0.185 | 0.205 | 0.222 | 0.288 | 0.330 | 0.319 | 0.192 | 0.395 |
编号 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 |
μg/L | 0.173 | 0.396 | 0.390 | 0.354 | 0.428 | 0.242 | 0.250 | 0.202 | 0.122 | 0.091 |
编号 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 |
μg/L | 0.228 | 0.438 | 0.258 | 0.282 | 0.285 | 0.252 | 0.286 | 0.310 | 0.341 | 0.157 |
编号 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 |
μg/L | 0.343 | 0.424 | 0.301 | 0.301 | 0.096 | 0.203 | 0.276 | 0.327 | 0.327 | 0.144 |
编号 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 |
μg/L | 0.337 | 0.335 | 0.255 | 0.220 | 0.200 | 0.095 | 0.143 | 0.117 | 0.381 | 0.374 |
编号 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 |
μg/L | 0.262 | 0.430 | 0.245 | 0.175 | 0.369 | 0.353 | 0.196 | 0.268 | 0.276 | 0.203 |
编号 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 |
μg/L | 0.433 | 0.251 | 0.298 | 0.096 | 0.156 | 0.229 | 0.206 | 0.265 | 0.170 | 0.124 |
编号 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 |
μg/L | 0.332 | 0.416 | 0.219 | 0.246 | 0.288 | 0.304 | 0.369 | 0.409 | 0.312 | 0.096 |
编号 | 81 | 82 | 83 | 84 | 85 | 86 | 87 | 88 | 89 | 90 |
μg/L | 0.106 | 0.126 | 0.381 | 0.391 | 0.155 | 0.420 | 0.315 | 0.172 | 0.258 | 0.253 |
编号 | 91 | 92 | 93 | 94 | 95 | 96 | 97 | 98 | 99 | 100 |
μg/L | 0.305 | 0.355 | 0.127 | 0.241 | 0.097 | 0.228 | 0.448 | 0.397 | 0.121 | 0.382 |
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