CN104984479B - Suppression system and suppressing method that electronics clashes to specific cell - Google Patents
Suppression system and suppressing method that electronics clashes to specific cell Download PDFInfo
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- CN104984479B CN104984479B CN201510247391.1A CN201510247391A CN104984479B CN 104984479 B CN104984479 B CN 104984479B CN 201510247391 A CN201510247391 A CN 201510247391A CN 104984479 B CN104984479 B CN 104984479B
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Abstract
The present invention discloses suppression system and suppressing method of a kind of electronics head-on collision to specific cell, specific cell suppression system head-on collision arrow includes voltage regulating rectification circuit, frequency control circuit, waveform translation circuit and multistage amplifier circuit, voltage regulating rectification circuit is oscillating circuit, electric current is transformed into low pressure unidirectional pulse electric current in voltage regulating rectification circuit, medium frequency electric is transformed into low-frequency pulse electron stream by frequency control circuit, negative pole unidirectional pulse electric current is converted into unidirectional square wave current by waveform translation circuit again, last multistage amplifier circuit is by voltage amplification, and use nichrome electrode needle-discharging, emitted disk sends high energy displacement electron field strength, through air, hydrogen, hydrone is transmitted to entire patient.Enter each system of human body and organ through breathing.By designing the electric field of a kind of certain strength and frequency, suppress the formation of uncontrolled specific cell spindle during mitosis is carried out, so as to promote the apoptosis of division stage specific cell, normal cell is unaffected in the electric field.
Description
Technical field
The present invention relates to the technical field that specific cell suppresses, more particularly to a kind of suppression of electronics head-on collision to specific cell
System and suppressing method.
Background technology
Since the 80's of 20th century, electric pulse is widely used in biological and medical field, such as killing microorganism,
Cell fusion, gene transfection, treatment of cancer etc..In terms for the treatment of of cancer, a kind of method with fastest developing speed is electrochemotherapy.
Its cardinal principle is that effect of the electric field to cell can make cell membrane occur instantaneous perforation, so that by cancer therapy drug as rich Lay is mould
Element is delivered to intracellular.Although electrochemotherapy largely reduces the drug dose of patient, not fundamentally
Break away from toxic side effect of the medicine to patient in itself.And the development of electroporation then provides possibility for treatment of cancer without medicine
Property.It is electrotechniical to develop the adjustable extent for having widened the parameters such as the impulse waveform, pulsewidth, electric-field intensity of electric pulse, electric-field strength
Degree can reach hundreds of kilovolts/cm, and pulsewidth shortens to nanosecond by traditional microsecond, Millisecond.Sound of the cell to electric pulse
Electric pulse field parameter such as electric-field intensity, pulse width etc. should be depended on.When electric-field intensity is 0.1~1 kV, pulsewidth is 100 μ s
When, electric pulse can make cell occur electroporation, i.e. the lower cell membrane of electric pulse effect produces temporary micropore, and permeability increases,
It is easy to intracellular transmission medicine, genetic material, protein and other macromoleculars.The meeting again after electric pulse effect of these micropores
It is automatic to close, therefore electroporation is a reversible process.When electric-field intensity is that MV/cm, pulsewidth reach 10 ns grades,
Electric field can then influence intracellular signal transduction and intracellular structure around cell membrane, and this process is referred to as endocellular electricity processing.Mesh
Before, electroporation is applied to electrochemotherapy, and intracellular electric treatment then provides to adjust the structure of cell interior with function
May.Whether also there are other bioelectrical effects so between both！Answer is affirmative.
With electrotechniical development, researchers can apply higher electric-field intensity and narrower pulsewidth to cell.
It is 10~100 kV/cm successively to occur in that electric-field intensity, and pulsewidth is less than 1 μ s nanosecond pulsed electric field, ultra-short wave pulse electricity
Field, submicrosecond high intense pulsed electric field.Compared with traditional electroporation, these electric pulse energy inducing cells produce entirely different life
The processing of thing electrical effect, i.e. endocellular electricity.According to Buescher etc. description, intracellular organelle film, nuclear membrane can occurs
Perforate and the increased electric pulse of membrane permeability is possessed following feature:1. pulse rise time is less than intracellular organelle
The charging interval of film;2. pulsewidth be greater than organelle film charging interval and less than cell membrane charging interval;3. electric-field intensity
The charging complete during pulse application of organelle film can be made.This process is referred to as endocellular electricity processing.
As previously described, cell is counted as a circuit equivalent model during electroporation, and cell membrane is electric capacity, and cytoplasm is
Conductance.And in the electric pulse induced endocellular electricity processing of more short pulse duration, intracellular membrane structure is organelle film and nucleus
Film must just take in.Wherein cell membrane and organelle film are electric capacity, and the cytoplasm that can be led is electricity with cell nuclearplasm
Lead, endocellular electricity processing can be used for a series of in inducing cell with the feature for making intracellular organelle occur electroporation
Calcium ion release, gene expression variation, DNA and the core conformational change in physiological effect such as cellular calcium storehouse etc..
Based on above-mentioned theory with putting into practice conclusion, suppress the division of specific cell using the effect of electric field, so as to suppress cancer
The diffusion of disease is just possibly realized.
The content of the invention
For weak point present in above-mentioned technology, the present invention provides a kind of practical, electronics pair for working well
Hit the suppression system and suppressing method to specific cell.
A kind of electronics head-on collision of the present invention is made up of to the suppression system of specific cell the electric field formed in head-on collision arrow；
Cell includes cell membrane, cytoplasm and nucleus, it is necessary to a kind of specific proteins in fission process, this
A little protein are freely floated in cytoplasm, and these special protein are the part that a large amount of electric charges are carried in human body, cell point
Split early stage, nuclear division, Chromosomal arrangement is in cell, and those protein materials are sorted by three-dimensional, and end points is connected, and is formed
Chain, will further connect inhereditary material, and these inhereditary materials are then pulled into two cells from a cell, here it is
One cancer cell division generates the process of two, and two become four, the tumour then increased without limitation；
In the cavity that cancer cell is placed in head-on collision arrow, the downward launching electronics formation electric field of transmitting disk, if electric field is uniform
, discontinuously active force round about can cause charged molecule parallel to the motion of direction of an electric field, when electric field frequency is enough
Height, these molecular motions can slow down, for dipole, because the charged cathode and anode of its molecule are separation, it is just
It can match with direction of an electric field, and holding position is constant；All charged molecules including dipole, in uneven exchange
Then it can all be moved in electric field to the high direction of electric field density；In common Unseparated Cell, electric field be substantially it is uniform, because
This, the net electric field force suffered by charged molecule and dipole is nearly free from motion；And the specific cell for quickly dividing, having
Silk telophase, because division ditch splits two daughter cells, narrow cytoplasm connection is formed, electric field is near connecting portion
Uneven, polar molecule will bradykinesia；
Effect of the spindle in mitosis is that the daughter chromosome after duplication is evenly distributed in two daughter cells, this
During one, small polar molecule-tubulin polymerization is in strip the inhereditary material that subunit extends to cell central array
It is interior, and combined with chromosome；In no mitotic cell of progress, although electric field is uniform, the meeting of micro-pipe subunit
It is arranged in parallel according to direction of an electric field, act on the electric field force in cell subunit and prevent them from reaching the row realized needed for polymerization
Column direction, therefore mitosis can occur abnormal long-time and stop；Tubulin gathers because being caused by sufficiently strong electric field force
Closing obstacle will cause micro-pipe formation to be obstructed；Spindle formation obstacle can all occur for all cells, spend the thin of mitosis metaphase
Born of the same parents, its cell membrane, which will take place to hang, to be split, and two daughter chromosomes are pulled into cell the two poles of the earth, in mitosis final step born of the same parents
In the fission process of matter, division ditch is ultimately formed, and two cells are finally completely separable；And this narrow cell membrane connection
Place just produces a hourglass-like inhomogeneous field completely different with Unseparated Cell, during cytokinesis, electric field density
Highest position is located at the center of narrow positions, in the case, the field strength of concentration cause all electric charges and dipole by
It is moved to the effect of unidirectional electric field force to division ditch；Intracellular polar molecule and dipole are because by sufficiently strong electric field
The influence of power and towards division ditch movement, this will cause the disorder of intracellular structure, produce cytoclasis effect, and then the opposite sex is thin
Born of the same parents' division is suppressed；
Specific cell suppression system head-on collision arrow includes operator control panel, voltage regulating rectification circuit, frequency control circuit, waveform conversion
Circuit, electric current commutating circuit and multistage amplifier circuit, voltage regulating rectification circuit are oscillating circuit, and it is whole that 220V exchanges are electrically accessed pressure regulation
It is transformed into low pressure unidirectional pulse electric current through alternating current in current circuit, medium frequency electric is transformed into low-frequency pulse by frequency control circuit
Stream, then unidirectional pulse electric current is converted into unidirectional square wave current by waveform translation circuit, last multistage amplifier circuit puts electric current
Greatly, and sent from transmitting disk and be delivered to entire patient；Multistage amplifier circuit always by 8 grades of amplifications, boosts to 12V voltages
50000-80000V；Operator control panel is electrically connected with each circuit respectively, and each circuit is controlled, the multistage amplifier circuit
It is contained in the multistage enlarged cavity beside electronics head-on collision arrow；
Parameters index in the specific cell suppression system running is as follows：
Frequency is to influence one of important parameter of waveform electric field therapeutic effect, less than the DeGrain of 1kHz electric fields, though
There is stimulation, but will soon disappear, more than MHz grades of high-frequency electric field can produce fuel factor, it is big to patient body side effect,
The frequency continuously adjustabe of the specific cell suppression system, shake frequency frequency-tuning range covering 0.9Hz-520.2Hz；
Direction of an electric field influences effect of the electric field to cell, when direction of an electric field is consistent with cell division direction of principal axis, lethality
Maximum, and single one direction treatment can not bring about tangible results, the system takes the electric field of multi-direction number sequentially to rotate
Angle between effect, multi-direction electric field is 45 ~ 90 °；
The environment temperature of the system is 5 DEG C -40 DEG C, and relative humidity is less than or equal to 80%, and system maximum output voltage is
24V, the peak value of output voltage is 28V, and rated load impedance is 800, and the individual pulse maximum output energy of electrode is
0.45mJ, time set scope is 60s-360s, is reached after the setting time, automatically cuts off treatment output, and sound, light prompting；
The battery lead plate of the system is nichrome electrode needle, and wherein nickel element mass fraction is 55%-60%, chromium quality
Fraction is 20%-30%, and battery lead plate profile is strip flat board or bent plate, there is energization wiring hole at the two ends of plate electrode plate, curved
The battery lead plate of plate includes two side plates and a top plate, and two side plates and a top plate are integrated the ∏ shape bending plates of composition,
The following bottom plate for respectively having an outward bending of biside plate, its longitudinal profile is in handle-shaped, and nichrome electrode needle is from battery lead plate top
Stretched out in plate, a diameter of 3-5mm of alloy electrode pin, the spacing between adjacent alloys electrode needle is 20-40mm.
Wherein, voltage regulating rectification circuit is made up of automatic coupling voltage regulator, and frequency control circuit is made up of frequency modulator, automatic coupling voltage regulator
Output end electrically connected with the input of frequency modulator, frequency modulator regulation and control frequency after from output end deliver current to waveform conversion electricity
Road, the compound action through multidirectional circuit and amplifying circuit, head-on collision electronics electric field is transmitted into entire patient from battery lead plate.
Wherein, the suppression system also includes power circuit；The power circuit includes 12V rechargeable batteries and boosting electricity
Road, the 12V rechargeable batteries are electrically connected with the waveform translation circuit and multistage amplifier circuit.
Wherein, the direction rotation per second of the electronics head-on collision electric field 1 time, direction of rotation is counterclockwise.
The invention discloses a kind of suppressing method of electronics head-on collision to specific cell, concrete practice is worked head-on collision arrow
Cheng Zhong；
First, cancer cell is placed in the cavity of head-on collision arrow, the downward launching electronics formation electric field of transmitting disk, if electric field is
Uniformly, active force discontinuously round about can cause charged molecule parallel to the motion of direction of an electric field, work as electric field frequency
Sufficiently high, these molecular motions can slow down, for dipole, because the charged cathode and anode of its molecule are separation,
It will match with direction of an electric field, and holding position is constant；All charged molecules including dipole, uneven
Then it can all be moved in AC field to the high direction of electric field density；In common Unseparated Cell, electric field is substantially uniform
, therefore, the net electric field force suffered by charged molecule and dipole is nearly free from motion；And it is thin for the opposite sex quickly divided
Born of the same parents, in mitosis telophase, because division ditch splits two daughter cells, form narrow cytoplasm connection, attached in connecting portion
Nearly electric field is uneven, and polar molecule will bradykinesia；
Cell includes cell membrane, cytoplasm and nucleus, it is necessary to a kind of specific proteins in fission process, this
A little protein are freely floated in cytoplasm, and these special protein are the part that a large amount of electric charges are carried in human body, cell point
Split early stage, nuclear division, Chromosomal arrangement is in cell, and those protein materials are sorted by three-dimensional, and end points is connected, and is formed
Chain, will further connect inhereditary material, and these inhereditary materials are then pulled into two cells from a cell, here it is
One cancer cell division generates the process of two, and two become four, the tumour then increased without limitation；
It is by the daughter chromosome mean allocation after duplication to carry effect of the spindle of this kind of protein in mitosis
Into two daughter cells, during this, small polar molecule-tubulin polymerization is in strip subunit and extends to cell
In the inhereditary material of central array, and combined with chromosome；In no mitotic cell of progress, although electric field is uniform
, but micro-pipe subunit can be arranged in parallel according to direction of an electric field, and the electric field force acted in cell subunit prevents them from arriving
Up to the orientation realized needed for polymerizeing, therefore mitosis can occur abnormal long-time and stop；Tubulin is because by enough
Strong electric field force and cause polymerization obstacle that micro-pipe formation will be caused to be obstructed；Spindle formation obstacle, degree can all occur for all cells
The cell of mitosis metaphase is crossed, its cell membrane, which will take place to hang, to be split, and two daughter chromosomes are pulled into cell the two poles of the earth,
In the fission process of mitosis final step kytoplasm, division ditch is ultimately formed, and two cells are finally completely separable；And this
Individual narrow cell membrane junction just produces a hourglass-like inhomogeneous field completely different with Unseparated Cell, in kytoplasm point
During splitting, electric field density highest position is located at the center of narrow positions, and in the case, the field strength of concentration causes institute
There are electric charge and dipole to be moved to division ditch by unidirectional electric field force；Intracellular polar molecule and dipole because
Influenceed and moved towards division ditch by sufficiently strong electric field force, this will cause the disorder of intracellular structure, produce cell and break
Bad effect, so specific cell division suppressed；
The circuit regulative mode of the specific cell suppressing method is that 220V exchanges are electrically accessed in head-on collision arrow voltage regulating rectification circuit
It is transformed into low pressure unidirectional pulse electric current through alternating current, medium frequency electric is transformed into low frequency pulse current by frequency control circuit, then by ripple
Unidirectional pulse electric current is converted to unidirectional square wave current by fractal transform circuit, and last multistage amplifier circuit is by Current amplifier, and from hair
Penetrate disk and send and be delivered to entire patient；
Parameters index in specific cell suppressing method head-on collision arrow running is as follows：
Frequency is to influence one of important parameter of waveform electric field therapeutic effect, less than the DeGrain of 1kHz electric fields, though
There is stimulation, but will soon disappear, more than MHz grades of high-frequency electric field can produce fuel factor, it is big to patient body side effect,
The frequency continuously adjustabe of the specific cell suppression system, shake frequency frequency-tuning range covering 0.9Hz-520.2Hz；
Direction of an electric field influences effect of the electric field to cell, when direction of an electric field is consistent with cell division direction of principal axis, lethality
Maximum, and single one direction treatment can not bring about tangible results, the system takes the electric field of multi-direction number sequentially to rotate
Angle between effect, multi-direction electric field is 45 ~ 90 °；
The environment temperature for operating this method is 5 DEG C -40 DEG C, and relative humidity is less than or equal to 80%, head-on collision arrow maximum output electricity
Press as 24V, the peak value of output voltage is 28V, and rated load impedance is 800, and the individual pulse maximum output energy of electrode is
0.45mJ, time set scope is 60s-360s, is reached after the setting time, automatically cuts off treatment output, and sound, light prompting；
The battery lead plate of the head-on collision arrow is nichrome battery lead plate, and wherein nickel element mass fraction is 55%-60%, chromium matter
Amount fraction is 20%-30%, and battery lead plate profile is strip flat board or bent plate, has energization wiring hole at the two ends of plate electrode plate,
The nickel chromium triangle battery lead plate of bent plate includes two side plates and a top plate, and two side plates and a top plate are integrated the ∏ shapes bending of composition
Plate, in the bottom plate for respectively having an outward bending below of biside plate, its longitudinal profile is in handle-shaped, there is electrical connection hole on bottom plate,
Steel mesh is provided with the cavity of battery lead plate, steel mesh is weaved into by nichrome wire, a diameter of 3-5mm of alloy wire, adjacent alloys line
Between spacing be 20-40mm, the appearance and size of steel mesh and the size of battery lead plate are adapted.
Wherein, the circuit during voltage regulating rectifier is provided by automatic coupling voltage regulator, and the circuit in frequency regulation process is by frequency modulation
Device is constituted, and the output end of automatic coupling voltage regulator electrically connect with the input of frequency modulator, and frequency modulator regulates and controls electricity after frequency from output end
Stream is delivered to waveform translation circuit, the compound action through multidirectional circuit and amplifying circuit, and head-on collision electronics electric field is launched from battery lead plate
To entire patient.
Wherein, the suppressing method also needs to power circuit support；The power circuit includes 12V rechargeable batteries and liter
Volt circuit, the 12V rechargeable batteries are electrically connected with the waveform translation circuit and multistage amplifier circuit.
Wherein, the direction rotation per second of the electronics head-on collision electric field 1 time, direction of rotation is counterclockwise.
The beneficial effects of the invention are as follows：
Compared with prior art, electronics of the invention head-on collision is a kind of specific strong by designing to the suppression system of specific cell
Degree and the electric field of frequency, so that suppress the formation of the fast specific cell of split speed spindle during mitosis is carried out,
So as to the apoptosis of induced fission phase cell.The system provides a kind of new thinking for the treatment of cancer.Cancer in the electric field is thin
Born of the same parents' apoptosis, but normal structure will not be impacted, and the function of normal structure is thus ensure that while specific cell is killed.
The suppressing method belongs to high-energy physics therapy, completely painless, will not also produce negative interaction, patient is solved well
The security problem of quality of life in cancer treatment procedure.
Brief description of the drawings
Fig. 1 is the structure chart of the electronics head-on collision arrow used in the system；
Fig. 2 is the circuit connection diagram in the suppression system of electronics head-on collision specific cell；
Fig. 3 is normal condition schematic diagram of the specific cell in without electric field environment；
Fig. 4 is specific cell mitosis schematic diagram at initial stage in without electric field environment；
Fig. 5 is specific cell mitosis metaphase schematic diagram in without electric field environment；
Fig. 6 is specific cell mitosis telophase schematic diagram in without electric field environment；
Fig. 7 is specific cell mitosis schematic diagram at initial stage under the system running status；
Fig. 8 is specific cell mitosis metaphase schematic diagram under the system running status；
Fig. 9 is the amplifying circuit schematic diagram in the suppression system of electronics head-on collision specific cell；
Figure 10 is the sedate pathologic finding report in 2010 of the 5th embodiment Liao；
Figure 11 is the sedate CT examination report in 2011 of the 5th embodiment Liao；
Figure 12 is the great 2010 CT diagnosis report lists of sixth embodiment plum；
Figure 13 is that sixth embodiment plum expands the CT diagnosis report lists after therapeutic trial in 2011；
Figure 14 is the great 2011 blood sample reports 1 after electronics head-on collision arrow treatment of sixth embodiment plum；
Figure 15 is the great 2011 blood sample reports 2 after electronics head-on collision arrow treatment of sixth embodiment plum.
Mitotic specific cell is carried out in suppression system of the present invention in telophase apoptosis.
Main element symbol description is as follows：
10th, transmitting disk 11, operator control panel
12nd, multistage enlarged cavity.
Embodiment
In order to more clearly state the present invention, the present invention is further described below in conjunction with the accompanying drawings.
First embodiment
The suppression system that a kind of electronics of the present invention clashes to specific cell, as shown in figure 1, by the electricity formed in head-on collision arrow
Field composition；
Fig. 3-6 are referred to, cell is included in cell membrane, cytoplasm and nucleus, fission process, it is necessary to a kind of special
Different protein, these protein are freely floated in cytoplasm, and these special protein are to carry a large amount of electric charges in human body
Part, prophase of cell division, nuclear division, Chromosomal arrangement is in cell, and those protein materials are sorted by three-dimensional, end
Point is connected, and forms chain, will further connect inhereditary material, these inhereditary materials are then pulled into two from a cell
Cell, here it is a cancer cell division generates the process of two, two become four, the tumour then increased without limitation；
Fig. 7-8 are further regarded to, in the cavity that cancer cell is placed in head-on collision arrow, the transmitting downward launching electronics of disk 10 form electricity
, if electric field is uniform, discontinuously active force round about can cause charged molecule parallel to the fortune of direction of an electric field
Dynamic, when electric field frequency is sufficiently high, these molecular motions can slow down, for dipole because the charged cathode of its molecule and
Anode is separation, and it will match with direction of an electric field, and the constant i.e. state shown in Fig. 7 in holding position；Exist including dipole
Interior all charged molecules, then can all be moved in uneven AC field to the high direction of electric field density；Common non-
In somatoblast, electric field is substantially uniform, and therefore, the net electric field force suffered by charged molecule and dipole is nearly free from fortune
Move state as shown in Figure 8；And the specific cell for quickly dividing, in mitosis telophase, because division ditch splits two sons
Cell, forms narrow cytoplasm connection, near connecting portion electric field be it is uneven, polar molecule will bradykinesia,
Complete cell just apoptosis can not be split at all；
Effect of the spindle in mitosis is that the daughter chromosome after duplication is evenly distributed in two daughter cells, this
During one, small polar molecule-tubulin polymerization is in strip the inhereditary material that subunit extends to cell central array
It is interior, and combined with chromosome；In no mitotic cell of progress, although electric field is uniform, the meeting of micro-pipe subunit
It is arranged in parallel according to direction of an electric field, act on the electric field force in cell subunit and prevent them from reaching the row realized needed for polymerization
Column direction, therefore mitosis can occur abnormal long-time and stop；Tubulin gathers because being caused by sufficiently strong electric field force
Closing obstacle will cause micro-pipe formation to be obstructed；Spindle formation obstacle can all occur for all cells, spend the thin of mitosis metaphase
Born of the same parents, its cell membrane, which will take place to hang, to be split, and two daughter chromosomes are pulled into cell the two poles of the earth, in mitosis final step born of the same parents
In the fission process of matter, division ditch is ultimately formed, and two cells are finally completely separable；And this narrow cell membrane connection
Place just produces a hourglass-like inhomogeneous field completely different with Unseparated Cell, during cytokinesis, electric field density
Highest position is located at the center of narrow positions, in the case, the field strength of concentration cause all electric charges and dipole by
It is moved to the effect of unidirectional electric field force to division ditch；Intracellular polar molecule and dipole are because by sufficiently strong electric field
The influence of power and towards division ditch movement.This will cause the disorder of intracellular structure, produce cytoclasis effect, and then the opposite sex is thin
Born of the same parents' division is suppressed；
Further referring to Fig. 1, Fig. 2 and Fig. 9, specific cell suppression system head-on collision arrow includes operator control panel 11, pressure regulation
Rectification circuit, frequency control circuit, waveform translation circuit, electric current commutating circuit and multistage amplifier circuit, voltage regulating rectification circuit
For oscillating circuit, 220V exchanges are electrically accessed in voltage regulating rectification circuit is transformed into low pressure unidirectional pulse electric current through alternating current, and frequency is adjusted
Medium frequency electric is transformed into low frequency pulse current by control circuit, then unidirectional pulse electric current is converted into unidirectional square wave by waveform translation circuit
Electric current, last multistage amplifier circuit sends by Current amplifier, and from transmitting disk 10 and is delivered to entire patient；Multistage amplifier circuit will
12V voltages by 8 grades of amplifications, boost to 50000-80000V always；Operator control panel 11 is electrically connected with each circuit respectively, and right
Each circuit is controlled, and the multistage amplifier circuit is contained in the multistage enlarged cavity 12 beside electronics head-on collision arrow；
Parameters index in the specific cell suppression system running is as follows：
Frequency is to influence one of important parameter of waveform electric field therapeutic effect, less than the DeGrain of 1kHz electric fields, though
There is stimulation, but will soon disappear, more than MHz grades of high-frequency electric field can produce fuel factor, it is big to patient body side effect,
The frequency continuously adjustabe of the specific cell suppression system, shake frequency frequency-tuning range covering 0.9Hz-520.2Hz；
Direction of an electric field influences effect of the electric field to cell, when direction of an electric field is consistent with cell division direction of principal axis, lethality
Maximum, and single one direction treatment can not bring about tangible results, the system takes the electric field of multi-direction number sequentially to rotate
Angle between effect, multi-direction electric field is 45 ~ 90 °；
The environment temperature of the system is 5 DEG C -40 DEG C, and relative humidity is less than or equal to 80%, and system maximum output voltage is
24V, the peak value of output voltage is 28V, and rated load impedance is 800, and the individual pulse maximum output energy of electrode is
0.45mJ, time set scope is 60s-360s, is reached after the setting time, automatically cuts off treatment output, and sound, light prompting；
The battery lead plate of the system is nichrome electrode needle, and wherein nickel element mass fraction is 55%-60%, chromium quality
Fraction is 20%-30%, and battery lead plate profile is strip flat board or bent plate, there is energization wiring hole at the two ends of plate electrode plate, curved
The battery lead plate of plate includes two side plates and a top plate, and two side plates and a top plate are integrated the ∏ shape bending plates of composition,
The following bottom plate for respectively having an outward bending of biside plate, its longitudinal profile is in handle-shaped, and nichrome electrode needle is from battery lead plate top
Stretched out in plate, a diameter of 3-5mm of alloy electrode pin, the spacing between adjacent alloys electrode needle is 20-40mm.
In the present embodiment, voltage regulating rectification circuit is made up of automatic coupling voltage regulator, and frequency control circuit is made up of frequency modulator, from
The output end of coupling pressure regulator is electrically connected with the input of frequency modulator, and ripple is delivered current to from output end after frequency modulator regulation and control frequency
Fractal transform circuit, the compound action through multidirectional circuit and amplifying circuit, head-on collision electronics electric field is transmitted into entire patient from battery lead plate.
In the present embodiment, the suppression system also includes power circuit；The power circuit includes 12V rechargeable batteries
And booster circuit, the 12V rechargeable batteries electrically connect with the waveform translation circuit and multistage amplifier circuit.
In the present embodiment, the direction rotation per second of the electronics head-on collision electric field 1 time, direction of rotation is counterclockwise.
Second embodiment
For the experiment of GBM illness patients
Patient be all GBM recurrence, they once received operation, the radiotherapy of high intensity and First-line chemotherapy, then they
All it have failed, tumour still recurs.
First group receives second-line chemotherapy, and contrast does not receive the patient for the treatment of completely, it is contemplated that can extend one times of survival life-span；
Second group, only receive the treatment of oncotherapy electric field, two combination radiotherapy groups and oncotherapy electric field treatment group are expected the longevity
Life is the same, it is essential however that the control group for receiving electronics head-on collision arrow is not subject to the side effect of any chemotherapy, is not ached
Bitterly, do not infect, without nausea and diarrhoea, constipation or fatigue, these possible symptoms.
3rd embodiment
Treat diabetes clinical research
Dong so-and-so, female, 58 years old, 15 years diabetes medical histories（II type）, in July, 2001, find double lower limb acroanesthesia, both feet
There is blister sample ulcer in toe, gradually spreads, and black hole occurs in right crus of diaphragm little finger of toe, suppurates more than, it is impossible to walk, three or four toe necrosis.
Right leg is numb, and doctor advocates to do high amputation operation.Patient receives the treatment of electronics colliding technique, using inhalation, blows pouring method, stops
Use any medicine.After two months, blood urine sugar is normal.Treated through three months, right lower extremity healing rehabilitation is concurrent caused by diabetes
Disease is also wholly absent.Patient is paid a return visit after 1 year, is all gone well.
Fourth embodiment
Mr. Wang, female 60 years old, suffers from psoriasis more than 40 years, does not stop treating though warp is multi-party, always recurrent exerbation, Huan Zheyi
Lose treatment confidence.In March, 2006, receive electronics head-on collision treatment, daily 16 hours, by bimestrial treatment, be discharged from hospitals upon recovery.
During treatment, without diet control.Patient is paid a return visit, is all gone well so far, no recurrence.
5th embodiment
Refering to Figure 10-11, Liao so-and-so, man, 27 years old, in May, 2010 moved in Hospital of Southern Medical University, passes through light microscopic
It was observed that its stomach swells, thing oncocyte is in fusiformis, intensive；Slight atypia, nuclear fission is diagnosed as low evil as 4 ~ 7/50HPP
Property GISTs.The CT examination report that in June, 2011 is carried out is shown, a huge mass shape occupancy focus is seen between liver stomach,
Finishing is owed at edge, and form is irregular, size about 12.2*9.3*10cm or so.Stomach and pancreas are caused form tube wall, disease by displacement is pushed
Stove is charged into forward in abdomen antetheca subcutaneous layer of fat and locally protrudes from body surface；It is unclear with coat of the stomach and the boundary of surrounding intestinal tube, with rear
Pancreas boundary is still distinguished.Focus density unevenness, most of is in soft-tissue density, and low density area and point, bar are dispersed at interior visible number
Shape densification shadow；The big portion of enhancing scanning focus slightly strengthens, and its interior low density area contrast is obvious.CT diagnose and impression be liver stomach between account for
Position venereal disease becomes, and considers that mesenchymoma possibility is big during with reference to clinical disease.Due to stomach mesenchymoma, therefore it dare not perform an operation always.
In March, 2013 is treated using the ECT electronics head-on collision arrow of Kang Xin Science and Technology Ltd.s, the electricity of three days of the arrow that clashed through the electronics
Son head-on collision with power supply treat feels good, after proceed trimestral treatment, stomach mesenchymoma is removed, is discharged from hospitals upon recovery substantially.
During treatment, without diet control.Patient is paid a return visit, is all gone well so far, no recurrence.
Specific embodiment six
Further regard to Figure 12-15, plum so-and-so, female, 41 years old, in November, 2010 was in the attached Beijing Cancer of the Capital University of Medical Sciences
Chest Enhanced CT inspection is carried out during hospitalize, report display, thorax is symmetrical, indulges diaphragm placed in the middle.Tracheae and leaf Duan Zhiqi
Pipe is unobstructed.Double pulmonary branches tracheae vascular bundles increase, disorder of walking, and the visible tubercle shadow of superior lobe of right lung, edge is rough, it is seen that multiple
Burr, and and pleaural adhesion, density is more uniform, and obvious reinforcing is had no after enhancing；Middle lobe of right lung, the visible patch shape of left lung tongue section are cloudy
Shadow, ground glass shadow.Vertical diaphragm is interior, double hilus pulumonis and right supraclavicular fossa are shown in several small lymph nodes.Heart, big vascular morphology, size have no
It is abnormal.Upper right pleura limitation plumpness adhesion, right side oblique segmentation is thickened, and thoracic cavity and pericardium have no obvious hydrops.Spleen increases, up to eight
Individual rib unit.Diagnostic result is upper right lung cancer, and double lung inflammations, vertical diaphragm and double hilus pulumonis, the several small lymph nodes of right supraclavicular fossa are shown,
Right side pleura localized thickening, splenomegaly.Entered to exercise using the ECT electronics head-on collision arrow of Kang Xin Science and Technology Ltd.s at 2 months 2011
Treated with property, chest Enhanced CT is carried out again, is as a result diagnosed as, focus slightly has diminution to upper right lung cancer than before, indulge diaphragm many
Hair small lymph node is shown have been reduced than before, right side pleura localized thickening.After proceed trimestral treatment, right upper lung
Carninomatosis stove reduces rapidly, is discharged from hospitals upon recovery.During treatment, without diet control.Patient is paid a return visit, is all gone well so far, no recurrence.
So, the present invention can effectively play the division for suppressing specific cell, while without any side effects.Although at present originally
The electronics head-on collision of invention can only be used the suppression system and suppressing method of specific cell as independent treatment system, but
It is that, by further research, more clinical tests are treated the system with method with conventional method combination, it will
Have broad prospects.
Disclosed above is only several specific embodiments of the present invention, but the present invention is not limited to this, any ability
What the technical staff in domain can think change should all fall into protection scope of the present invention.
Claims (2)
1. a kind of electronics head-on collision is to the suppression system of specific cell, it is characterised in that by the high electric field formed in head-on collision arrow
Composition；
Cell is included in cell membrane, cytoplasm and nucleus, fission process, it is necessary to a kind of specific proteins, the spy
Different protein is freely floated in cytoplasm, and the specific proteins carry a large amount of electric charges, prophase of cell division, nucleus point
Split, Chromosomal arrangement is in cell, and the protein in cell is sorted by three-dimensional, and end points is connected, and forms chain, will be further
Inhereditary material is connected, these inhereditary materials are then pulled into two cells from a cell, here it is a cancer cell division
The process of generation two；
In the cavity that cancer cell is placed in head-on collision arrow, the downward launching electronics formation electric field of transmitting disk, if electric field is uniform,
Active force round about can cause charged molecule to be moved parallel to direction of an electric field disconnectedly, when electric field frequency is sufficiently high,
These molecular motions can slow down, for dipole, because the charged cathode and anode of its molecule are separation, it will be with
Direction of an electric field matches, and holding position is constant；All charged molecules including dipole, in uneven AC field
In then can all be moved to the high direction of electric field density；In common Unseparated Cell, electric field be substantially it is uniform, therefore,
Net electric field force suffered by charged molecule is nearly free from motion；And the specific cell for quickly dividing, in mitosis telophase,
Because division ditch splits two daughter cells, narrow cytoplasm connection is formed, electric field is uneven, band near connecting portion
Electric molecule will bradykinesia；
Effect of the spindle in mitosis is that the daughter chromosome after duplication is evenly distributed in two daughter cells, this mistake
Tubulin polymerization in Cheng Zhong, cell is in strip subunit and extended in the inhereditary material of cell central array, and with dyeing
Body is combined；In no mitotic cell of progress, although electric field is uniform, the subunit of tubulin polymerization formation
Can be arranged in parallel according to direction of an electric field, act on the electric field force in subunit and prevent them from reaching the arrangement realized needed for polymerization
Direction, therefore mitosis can occur abnormal long-time and stop；Tubulin is because being caused polymerization by sufficiently strong electric field force
Obstacle will cause micro-pipe formation to be obstructed；Spindle formation obstacle can all occur for all cells, spend the cell of mitosis metaphase,
Its cell membrane, which will take place to hang, to be split, and two daughter chromosomes are pulled into cell the two poles of the earth, in mitosis final step kytoplasm
Fission process in, division ditch is ultimately formed, and two cells are finally completely separable；And this narrow cell membrane junction
A hourglass-like inhomogeneous field completely different with Unseparated Cell is just produced, during cytokinesis, electric field density is most
High position is located at the center of narrow positions, and in the case, the field strength of concentration causes all charged molecules by unidirectional
The effect of electric field force and to division ditch be moved；Intracellular charged molecule by sufficiently strong electric field force because being influenceed and direction
Divide ditch movement, this will cause the disorder of intracellular structure, produce cytoclasis effect, and then specific cell division is pressed down
System；
The head-on collision arrow includes operator control panel, voltage regulating rectification circuit, frequency control circuit, waveform translation circuit, electric current commutating circuit
And multistage amplifier circuit, voltage regulating rectification circuit is oscillating circuit, and 220V exchanges are electrically accessed in voltage regulating rectification circuit through alternating current
It is transformed into low pressure unidirectional pulse electric current, medium frequency electric is transformed into low frequency pulse current by frequency control circuit, then converts electricity by waveform
Unidirectional pulse electric current is converted to unidirectional square wave current by road, and last multistage amplifier circuit is by Current amplifier, and the electricity by amplifying
Stream is sent from transmitting disk and is delivered to entire patient, and operator control panel is electrically connected with each circuit respectively, and each circuit is controlled,
The multistage amplifier circuit is contained in the multistage enlarged cavity beside head-on collision arrow；
Parameters index in the suppression system running is as follows：
Frequency is to influence one of important parameter of waveform electric field therapeutic effect, less than the DeGrain of 1kHz electric fields, though spinosity
Swash effect, but will soon disappear, more than MHz grades of high-frequency electric field can produce fuel factor, big to patient body side effect, this is different
Property Carbazole alkaloid system frequency continuously adjustabe, shake frequency frequency-tuning range covering 0.9Hz-520.2Hz；
Direction of an electric field influences effect of the electric field to cell, when direction of an electric field is consistent with cell division direction of principal axis, and lethality is maximum,
And single one direction treatment can not bring about tangible results, the system takes the electric field sequentially turning effort of multi-direction number,
Angle between multi-direction electric field is 45~90 °；
The environment temperature of the system is 5 DEG C -40 DEG C, and relative humidity is less than or equal to 80%, and system maximum output voltage is 24V,
The peak value of output voltage is 28V, and rated load impedance is 800 Ω, and the individual pulse maximum output energy of electrode is 0.45mJ, fixed
When set scope to be 60s-360s, reach after the setting time, automatically cut off treatment output, and the prompting of sound, light；
The battery lead plate of the system is nichrome electrode needle, and wherein nickel element mass fraction is 55%-60%, chromium quality point
Number is 20%-30%, and battery lead plate profile is strip flat board or bent plate, there is energization wiring hole at the two ends of plate electrode plate, curved
The battery lead plate of plate includes two side plates and a top plate, and two side plates and a top plate are integrated the ∏ shape bending plates of composition,
The following bottom plate for respectively having an outward bending of biside plate, its longitudinal profile is in handle-shaped, and nichrome electrode needle is from battery lead plate top
Stretched out in plate, a diameter of 3-5mm of alloy electrode pin, the spacing between adjacent alloys electrode needle is 20-40mm；
Voltage regulating rectification circuit is made up of automatic coupling voltage regulator, and frequency control circuit is made up of frequency modulator, the output end of automatic coupling voltage regulator
Electrically connected with the input of frequency modulator, waveform translation circuit is delivered current to from output end after frequency modulator regulation and control frequency, through electricity
The compound action of commutating circuit and multistage amplifier circuit is flowed, head-on collision electronics electric field is transmitted into entire patient from battery lead plate；
The suppression system also includes power circuit；The power circuit includes 12V rechargeable batteries and booster circuit, the 12V
Rechargeable battery is electrically connected with the waveform translation circuit and multistage amplifier circuit.
2. electronics head-on collision according to claim 1 is to the suppression system of specific cell, it is characterised in that the electronics head-on collision
The direction rotation per second of electric field 1 time, direction of rotation produces metal Luminous Ring counterclockwise to assemble, high energy.
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| WO2017118390A1 (en) * | 2016-01-05 | 2017-07-13 | 吴翔 | Selectively manipulating molecule by using variable electric field, magnetic field or electromagnetic field |
| CN113368389B (en) * | 2021-06-08 | 2023-03-14 | 湖南安泰康成生物科技有限公司 | Equipment for inhibiting tumor proliferation by using electric field and control method and device thereof |
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| CN201625337U (en) * | 2009-09-14 | 2010-11-10 | 陈初生 | Low-frequency electro-therapeutic apparatus |
| CN102488967A (en) * | 2004-12-27 | 2012-06-13 | 斯坦顿有限公司 | Treating a tumor or the like with electric fields at different orientations |
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| CN201625337U (en) * | 2009-09-14 | 2010-11-10 | 陈初生 | Low-frequency electro-therapeutic apparatus |
| CN202844368U (en) * | 2012-08-28 | 2013-04-03 | 南京大学医学院附属鼓楼医院 | Tumor electric field therapeutic instrument |
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