CN104984395A - Autologous-tissue-based activity extract for soft tissue filling and preparation method - Google Patents
Autologous-tissue-based activity extract for soft tissue filling and preparation method Download PDFInfo
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- CN104984395A CN104984395A CN201510210097.3A CN201510210097A CN104984395A CN 104984395 A CN104984395 A CN 104984395A CN 201510210097 A CN201510210097 A CN 201510210097A CN 104984395 A CN104984395 A CN 104984395A
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Abstract
The invention relates to the field of soft tissue filling, and particularly discloses an autologous-tissue-based activity extract for soft tissue filling and a preparation method. The activity extract comprises an extracellular matrix derived from autologous skin dermis tissue of a patient to be treated. The extraction method for the activity extract comprises the following steps: the autologous skin dermis tissue of the patient to be treated is weighed, normal saline is added, and grinding is performed at low temperature to obtain a suspension; and low-temperature ultrasonic oscillation and centrifuging are performed on the suspension in sequence to obtain a centrifugate, and a milk white or grayish yellow middle layer centrifugate in the centrifugate is obtained and is the activity extract. The activity extract can be used for preparing a soft tissue filling agent and can be used for soft tissue filling, no foreign body reaction exists, the cost is low, and the effect is good.
Description
Technical field
The present invention relates to Soft-tissue operation field, specifically disclose a kind of activity extract for Soft-tissue operation based on autologous tissue and preparation method.
Background technology
Along with the improvement of people living condition, people propose more and more higher requirement to the appearance of beauty.People are unsatisfied with the face of oneself, need to carry out filling shaping to forehead, buccal, ear-lobe, wrinkle etc.This various Therapeutic Method is arisen at the historic moment, injection for curing because of its wound little, operating time is short, and postoperative effect is good and gain great popularity.
Injection beauty therapeutic mainly contains injection botulinum toxin and injection molding process thing two kinds of methods.Botulinum toxin is reached can be arrived reduce wrinkle object, instant effect by paralysis histrionic muscles, but hold time short, the shortcoming such as have little expression.
Business-like injection fillers thing mostly is hyaluronic acid, Evolution, Artecoll etc.Hyaluronic acid can be degraded, and holds time not long; Evolution main component is degradable artificial chemical synthetic material; Artecoll main component is bovine collagen and microsphere, and this several injection fillers thing obtains by synthetic or artificial treatment xenogenesis collagen, has different, can cause rejection with people autologous tissue.The autologous fat filling of rising in recent years is the fatty tissue extracted in autologous tissue, though be autologous tissue, the filling of injection autologous fat absorbs more obvious.
Summary of the invention
For problems of the prior art, the object of this invention is to provide a kind of activity extract for Soft-tissue operation based on autologous tissue and preparation method thereof, for foreign reaction during Soft-tissue operation, cheap and respond well.
In order to achieve the above object, the present invention is achieved by the following technical solutions.
(1) based on the activity extract for Soft-tissue operation of autologous tissue, it is characterized in that, comprise the extracellular matrix of the autologous skin dermal tissue deriving from therapist.
Described skin dermis comprises the dermal tissue of normal skin or the dermal tissue of Skin scar.Normal skin and Skin scar are excised by therapist row plastic operation respectively and obtain, and obtain the dermal tissue of normal skin and the dermal tissue of Skin scar respectively after removing epidermis.
Described Skin scar is the skin forming fibrous tissue containing normal knot that human body skin is formed after various wound, comprises common cicatrix, hypertrophic cicatrix or keloid.
Described extracellular matrix is the effective ingredient of activity extract, comprises the absorbable collagen of therapist, mucopolysaccharide, hyaluronic acid and/or protein.
(2) extracting method of the above-mentioned activity extract for Soft-tissue operation, is characterized in that, comprise the following steps: the autologous skin dermal tissue taking therapist, adds normal saline, and cryogrinding is suspension; By suspension successively through low temperature ultrasonic concussion, centrifugal after centrifugal liquid, in extracting centrifugal liquid, the middle level centrifugal liquid of milky or lark is described activity extract.
Described cryogrinding is ice bath grinding.
The condition of described low temperature ultrasonic concussion is ultrasonic vibration 10 minutes at 4 DEG C.
Described centrifugal condition be 1000 revs/min centrifugal 3 minutes.
(3) the above-mentioned activity extract for Soft-tissue operation is preparing the purposes in soft tissue filler.
The above-mentioned activity extract for Soft-tissue operation in use, the solvents that are autologous or the conventional use of business such as autologous platelet rich plasma, hyaluronic acid, gelatin can be added mix, form soft tissue filler, just can be injected directly into the position that therapist needs to carry out Soft-tissue operation.
After above-mentioned activity extract is injected to human body skin, first play a supporting role, the composition such as collagen, mucopolysaccharide, hyaluronic acid, protein wherein is progressively decomposed and absorbs and reconstruct as autologous tissue subsequently, long-term support is provided to skin, with treatment depressed scar, removal wrinkle, rich lip, pad chin, buccal filling, ear-lobe filling etc., the effect of shaping and beauty can be played.
Compared with prior art, activity extract of the present invention utilizes the effective ingredient of autologous skin dermal tissue to carry out tissue augmentation, and this extract comes from autologous patient tissue, can not produce foreign body reaction, safe and reliable.
Accompanying drawing explanation
Fig. 1 is the thickness chart of the test group of animal experiment one;
Fig. 2 is the thickness chart of the matched group of animal experiment one.
Fig. 3 is the thickness chart of the test group of animal experiment two;
Fig. 4 is the thickness chart of the matched group of animal experiment two.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further details, but the invention is not restricted to these embodiments.
Embodiment 1
The animal experiment one of activity extract of the present invention.
(1) choose experimental animal: choose the test in 6 6-8 age in week with nude mice, former skin of back is normal skin, observe skin thickness after injection.
(2) process of the test: get above-mentioned nude mice, lumbar injection pentobarbital sodium anesthesia 0.04mg/g body weight, uses 75% alcohol disinfecting by injection site.Activity extract of the present invention for 0.5ml is sucked in 1ml syringe for subsequent use.Activity extract is injected in dorsal sc on the left of nude mice, injects skin corium, multi-point injection, often some injection 0.02-0.05ml, after injection, epidermis turns white, and slightly heaves, about 0.2ml and injectable 1cm
2skin.Group is compared at right side co-located injection Isodose normal saline.Postoperative, with ice bag external application 10 minutes.
(3) clinical follow: through the analysis to 6 nude mices, through injection once, observes after one month, and local reaction without exception after injection, processed group skin is matched group projection comparatively, and fold is obviously less than matched group.Put to death test after January to get processed group and matched group with nude mice and make tissue slice and observe, find that test group skin thickness is comparatively large, P < 0.05, has remarkable significant difference.The measurement result of skin thickness is in table 1, Fig. 1 and Fig. 2.
Table 1 test group and matched group skin thickness
| Grouping | 4 weeks (μm) (Mean ± SD) |
| Experimental group (n=6) | 726.39±30.07 |
| Matched group (n=6) | 659.83±26.92 |
Zoopery proves, it is safe and reliable that activity extract of the present invention is used for Soft-tissue operation, and successful, has no adverse reaction.
Embodiment 2
The animal experiment two of activity extract of the present invention.
(1) choose experimental animal: choose the test in 6 6-8 age in week with white mice, former skin of back is normal skin, observe skin thickness after injection.
(2) process of the test: get above-mentioned white mice, lumbar injection pentobarbital sodium anesthesia 0.04mg/g body weight, rejects art district fur.Fat about 0.5ml in abdominal cavity fat grain is drawn with syringe.Its skin of abdomen of clip is about 1cm
2, extract activity extract according to activity extract extracting method of the present invention, this activity extract of 0.5ml sucked in 1ml syringe for subsequent use.Activity extract is injected in dorsal sc on the left of white mice, injects skin corium, multi-point injection, often some injection 0.02-0.05ml, after injection, epidermis turns white, and slightly heaves, about 0.2ml and injectable 1cm
2skin.Group is compared at the autologous fat of right side co-located injection Isodose.Postoperative, with ice bag external application 10 minutes.
(3) clinical follow: through the analysis to 6 white mice, through injection once, observes one month, and local reaction without exception after injection, processed group skin is matched group projection comparatively, and fold is less than matched group.Put to death test after January to get processed group and matched group with white mice and make tissue slice and observe, find that test group skin thickness is comparatively large, P < 0.05, has remarkable significant difference.The measurement result of skin thickness is in table 2, Fig. 3 and Fig. 4.
Table 2 test group and matched group skin thickness
| Grouping | 4 weeks (μm) (Mean ± SD) |
| Experimental group (n=6) | 1172.43±37.52 |
| Matched group (n=6) | 972.83±43.75 |
Zoopery proves, activity extract of the present invention is safe and reliable for Soft-tissue operation, and successful, has no adverse reaction, and absorbance is less than autologous fat filling, better effects if.
Embodiment 3
The animal experiment three of activity extract of the present invention.
(1) experimental animal is chosen: choose the test in 12 6-8 age in week with white mice, former skin of back is normal skin, has unchanged after observing injection.
(2) process of the test: get above-mentioned white mice, lumbar injection pentobarbital sodium anesthesia 0.04mg/g body weight, reject art district fur, its skin of abdomen of clip is about 1cm
2, extract activity extract according to activity extract extracting method of the present invention.This activity extract of 0.5ml is sucked in 1ml syringe for subsequent use.Activity extract is injected in dorsal sc on the left of 12 white mice, injects skin corium, multi-point injection, often some injection 0.02-0.05ml, after injection, epidermis turns white, and slightly heaves, about 0.2ml and injectable 1cm
2skin.And wherein 6 right side co-located injection Isodose bovine collagens, another 6 right side co-located injection Isodose polyvinyl alcohol cellular microspheres.Postoperative, with ice bag external application 10 minutes.
(3) clinical follow: through the analysis to 12 white mice, through injection once, observe two weeks, after injection, local is with or without abnormal response, the right side skin of part white mice has showed the symptoms such as red and swollen or punctation after injection bovine collagen or polyvinyl alcohol cellular microsphere in two weeks, injected the left side of autologous tissue's extract then without this symptom.
Embodiment 4
Prepare a kind of activity extract for Soft-tissue operation, specifically comprise the following steps:
Get the autologous common Skin scar of first therapist, after removing epidermis, obtain common cicatrix dermal tissue.Take autologous common cicatrix dermal tissue 0.75cm
2, at once put into the EP pipe of 4mL, with eye scissors, bulk scar tissue be cut into 1mm
2the fritter of left and right, then add 3mL normal saline, be placed in tissue grinder, under condition of ice bath, scar tissue ground and form suspension in 3 minutes; Again at 4 DEG C through ultrasonic vibration 10 minutes, and centrifugal 3 minutes with 1000 revs/min.In extracting centrifugal liquid, the middle level centrifugal liquid of milky or lark is activity extract.
Activity extract of the present invention is used for the face crinkle-removing of first therapist, specifically comprises the following steps:
(1) get 0.5mL activity extract, mix with the hyaluronic acid of 0.5mL4%, form soft tissue filler, for face crinkle-removing.
(2) injection site is facial corium layer, multi-point injection, each injection site injection 0.02mL, is injected to skin and slightly swells wrinkle and disappear and be advisable.
Embodiment 5
Prepare a kind of activity extract for Soft-tissue operation, specifically comprise the following steps:
Get the autologous hypertrophic cicatrix skin of second therapist, after removing epidermis, obtain hypertrophic cicatrix dermal tissue.Take autologous hypertrophic cicatrix dermal tissue 1cm
2, at once put into the EP pipe of 4mL, with eye scissors, bulk scar tissue be cut into 1mm
2the fritter of left and right, then add 3mL normal saline, be placed in tissue grinder, under condition of ice bath, scar tissue ground and form suspension in 3 minutes.Again at 4 DEG C through ultrasonic vibration 10 minutes, and centrifugal 3 minutes with 1000 revs/min.In extracting centrifugal liquid, the middle level centrifugal liquid of milky or lark is activity extract.
Activity extract of the present invention is used for the filling of second therapist depressed scar, specifically comprises the following steps:
(1) get 0.6mL activity extract, mix with 1.2mL platelet rich plasma extract, form soft tissue filler, for the filling of depressed scar.
(2) injection site is the subcutis of depressed scar, multi-point injection, each injection site injection 0.03mL, is injected to depressed area skin and slightly swells and be advisable.
Embodiment 6
Prepare a kind of activity extract for Soft-tissue operation, specifically comprise the following steps:
Get the autologous keloid of the 3rd therapist, after removing epidermis, obtain the dermal tissue of keloid.Take the skin dermis 1cm of keloid
2, at once put into the EP pipe of 4mL, with eye scissors, bulk scar tissue be cut into 1mm
2the fritter of left and right, then add 3mL normal saline, be placed in tissue grinder, under condition of ice bath, scar tissue ground and form suspension in 3 minutes.Again at 4 DEG C through ultrasonic vibration 10 minutes, and centrifugal 3 minutes with 1000 revs/min.In extracting centrifugal liquid, the middle level centrifugal liquid of milky or lark is activity extract.
Activity extract of the present invention is used for the 3rd therapist ear-lobe filling and chin filling, specifically comprises the following steps:
(1) get 0.8mL activity extract, mix with the liquid gelatin of 1.6mL4%, form soft tissue filler, for the filling of ear-lobe and chin.
(2) injection site is the skin corium of ear-lobe and chin, multi-point injection, each injection site injection 0.03mL, is injected to ear-lobe district and chin and slightly swells and be advisable.
Embodiment 7
Prepare a kind of activity extract for Soft-tissue operation, specifically comprise the following steps:
Get the autologous normal skin that the 4th therapist is discarded, after removing epidermis, obtain normal dermal tissue.Take normal skin dermis 1cm
2, at once put into the EP pipe of 4mL, with eye scissors, bulk dermal tissue be cut into 1mm
2the fritter of left and right, then add 3mL normal saline, be placed in tissue grinder, under condition of ice bath, this tissue grinder formed suspension in 3 minutes.Again at 4 DEG C through ultrasonic vibration 10 minutes, and centrifugal 3 minutes with 1000 revs/min.In extracting centrifugal liquid, the middle level centrifugal liquid of milky or lark is activity extract.
Activity extract of the present invention is used for the 4th therapist lip filling, buccal is plentiful, specifically comprises the following steps:
(1) get 0.6mL activity extract, mix with 1.2mL rich platelet blood blood plasma extract, form soft tissue filler, for for lip filling, buccal plentiful.
(2) injection site is lip dermal tissue, buccal skin corium, multi-point injection, each injection site injection 0.03mL, is injected to skin of lip and slightly swells and be advisable.
Although be described embodiment of the present invention above, the present invention is not limited to above-mentioned specific embodiments, and above-mentioned specific embodiments is only schematic, guiding, instead of restrictive.Those of ordinary skill in the art is under the enlightenment of this description, and when not departing from the scope that the claims in the present invention are protected, can also make a variety of forms, these all belong to the row of the present invention's protection.
Claims (9)
1. based on the activity extract for Soft-tissue operation of autologous tissue, it is characterized in that, comprise the extracellular matrix of the autologous skin dermal tissue deriving from therapist.
2. the extracting method of the activity extract for Soft-tissue operation according to claim 1, it is characterized in that, described skin dermis comprises the dermal tissue of normal skin or the dermal tissue of Skin scar, obtains after removing epidermis respectively by the normal skin of therapist or Skin scar.
3. the extracting method of the activity extract for Soft-tissue operation according to claim 1, it is characterized in that, described scar tissue is that the normal knot that human body skin is formed after various wound forms fibrous tissue, comprises common cicatrix, hypertrophic cicatrix or keloid.
4. the extracting method of the activity extract for Soft-tissue operation according to claim 1, is characterized in that, described extracellular matrix is the effective ingredient of activity extract, comprises the absorbable collagen of therapist, mucopolysaccharide, hyaluronic acid and/or protein.
5. for an extracting method for the activity extract of Soft-tissue operation, it is characterized in that, comprise the following steps: the autologous skin dermal tissue taking therapist, adds normal saline, and cryogrinding is suspension; By suspension successively through low temperature ultrasonic concussion, centrifugal after centrifugal liquid, in extracting centrifugal liquid, the middle level centrifugal liquid of milky or lark is described activity extract.
6. the extracting method of the activity extract for Soft-tissue operation according to claim 5, is characterized in that, described cryogrinding is ice bath grinding.
7. the extracting method of the activity extract for Soft-tissue operation according to claim 5, is characterized in that, the condition of described low temperature ultrasonic concussion is ultrasonic vibration 10 minutes at 4 DEG C.
8. the extracting method of the activity extract for Soft-tissue operation according to claim 5, is characterized in that, described centrifugal condition be 1000 revs/min centrifugal 3 minutes.
9. the activity extract for Soft-tissue operation according to claim 1 is preparing the purposes in soft tissue filler.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1868420A (en) * | 2005-05-27 | 2006-11-29 | 吕伟光 | Human soft tissue filler for injection, and its prepn. method |
| US20110020271A1 (en) * | 2009-05-20 | 2011-01-27 | Humacyte, Inc. | Elastin for soft tissue augmentation |
| CN102218162A (en) * | 2011-05-24 | 2011-10-19 | 山西奥瑞生物材料有限公司 | Preparation method of homologous acellular dermal matrix |
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- 2015-04-28 CN CN201510210097.3A patent/CN104984395A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1868420A (en) * | 2005-05-27 | 2006-11-29 | 吕伟光 | Human soft tissue filler for injection, and its prepn. method |
| US20110020271A1 (en) * | 2009-05-20 | 2011-01-27 | Humacyte, Inc. | Elastin for soft tissue augmentation |
| CN102218162A (en) * | 2011-05-24 | 2011-10-19 | 山西奥瑞生物材料有限公司 | Preparation method of homologous acellular dermal matrix |
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