CN104984388A - Skeletal system repairing material and preparation method thereof - Google Patents
Skeletal system repairing material and preparation method thereof Download PDFInfo
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- CN104984388A CN104984388A CN201510352125.5A CN201510352125A CN104984388A CN 104984388 A CN104984388 A CN 104984388A CN 201510352125 A CN201510352125 A CN 201510352125A CN 104984388 A CN104984388 A CN 104984388A
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Abstract
The invention discloses a skeletal system repairing material and a preparation method thereof. The skeletal system repairing material mainly comprises, by weight ratio, the following raw materials of 15-45 parts of collagen, 12-30 parts of glycosaminoglycan, 8-25 parts of hyaluronic acid, 3-20 parts of calcium phosphate, 2-16 parts of magnesium phosphate, 6-26 parts of chitosan, 2-15 parts of fiber mucoprotein, 8-27 parts of keratin and 35-65 parts of double distilled water. Compared with the prior art, the skeletal system repairing material and the preparation method thereof have the advantages that firstly, both the rigidity and tenacity of the prepared skeletal system repairing material can reach standards of human skeleton, and the skeletal system repairing material is suitable for being applied into repairing and reestablishing of the human skeleton; secondly, the prepared skeletal system repairing material can sustain the physiological stress experienced in the daily life; finally, the prepared skeletal system repairing material is free from any toxic and side effects.
Description
Technical field
The present invention relates to biomaterial for medical purpose field, particularly relate to a kind of skeletal system repair materials and preparation method thereof.
Background technology
Natural bone is collagen protein, comprises the composite biological material of the noncollagen protein organic facies of glycosaminoglycans and calcium phosphate.The hierarchy of its compound creates special engineering properties, comprises high hardness, intensity and fracture toughness, and these character make bone can bear the daily physiological stress stood.
Skeleton by the bone class apatite layer that formed in physical environment directly in conjunction with the calcium phosphate in human body; On the other hand, known collagen protein and the copolymer comprising collagen protein and other biological organism such as glycosaminoglycans are the optimum substrates of the attachment of various kinds of cell type and propagation.
Hydroxyapatite is the calcium phosphate being the most generally used as the composition in bone substitute materials.But compare with the calcium phosphate material of other forms such as brushite, tricalcium phosphate and OCP, hydroxyapatite is relatively insoluble material.Because the again absorption rate of this material in human body is low especially, so when preparing biomaterial, the dissolubility that apatite is relatively low is disadvantageous.
Such as, the calcium phosphate of hydroxyapatite is rigid material mechanically.But compare with natural bone, they are relative brittleness.Collagen protein is toughness material mechanically.But compare with nature bone and there is relatively low rigidity.The material comprising the copolymer of collagen protein and glycosaminoglycans has higher toughness and rigidity than independent collagen protein, but, compared with natural bone, still there is lower rigidity.
Summary of the invention
The technical problem solved: the invention provides a kind of skeletal system repair materials and preparation method thereof, to solve the not good enough problem of bone repair insufficient rigidity of material and toughness in prior art.
For achieving the above object, the present invention is by the following technical solutions:
A kind of skeletal system repair materials, makes by mass fraction proportioning primarily of following raw material: collagen protein 15 ~ 45 parts, glycosaminoglycan 12 ~ 30 parts, hyaluronic acid 8 ~ 25 parts, 3 ~ 20 parts, calcium phosphate, magnesium phosphate 2 ~ 16 parts, chitosan 6 ~ 26 parts, Fibronectin 2 ~ 15 parts, CK8 ~ 27 part, distilled water 35 ~ 65 parts.
As a modification of the present invention, described a kind of skeletal system repair materials is made by mass fraction proportioning primarily of following raw material: collagen protein 35 parts, glycosaminoglycan 28 parts, hyaluronic acid 21 parts, 16 parts, calcium phosphate, magnesium phosphate 12 parts, chitosan 21 parts, Fibronectin 12 parts, keratin 23 parts, distilled water 60 parts.
A preparation method for skeletal system repair materials, comprises following steps:
(1) collagen protein, Fibronectin and calcium phosphate are dissolved in distilled water, are heated to 28 ~ 37 DEG C and make slurry 1; Slurry 1 is deposited in a mold, is cooled to the temperature that slurry 1 is converted into multiple crystal or granule;
(2) by the crystal of acquisition in step (1) or heating particles to 80 ~ 90 DEG C, obtain composite porous 1, from mould, take out material;
(3) keratin, hyaluronic acid and magnesium phosphate are dissolved in distilled water, are heated to 28 ~ 37 DEG C and make slurry 2; Be deposited on after in mould until slurry 2, be cooled to-20 ~ 0 DEG C, after being all converted into multiple crystal or granule to slurry 2, be warming up to 40 ~ 65 DEG C with the speed of 10 ~ 15 DEG C/s, obtain composite porous 2, from mould, take out material;
(4) glycosaminoglycan and chitosan are heated to 95 ~ 132 DEG C, the one side of composite porous 1 and/or composite porous 2 is sprayed to after melting, and by staggered relatively for this face, thus by composite porous 1 and composite porous 2 adhesion-molded, obtained skeletal system repair materials.
As a preferred embodiment of the present invention, collagen protein, Fibronectin and calcium phosphate are dissolved in distilled water in (1) by step, are heated to 32 DEG C and make slurry 1; Slurry 1 is deposited in a mold, is cooled to the temperature that slurry 1 is converted into multiple crystal or granule.
As a preferred embodiment of the present invention, in step (2), by the crystal of acquisition in step (1) or heating particles to 86 DEG C, obtain composite porous 1, from mould, take out material.
As a preferred embodiment of the present invention, keratin, hyaluronic acid and magnesium phosphate are dissolved in distilled water in (3) by step, are heated to 31 DEG C and make slurry 2; Be deposited on after in mould until slurry 2, be cooled to-18 DEG C, after being all converted into multiple crystal or granule to slurry 2, be warming up to 62 DEG C with the speed of 14 DEG C/s, obtain composite porous 2, from mould, take out material.
As a preferred embodiment of the present invention, glycosaminoglycan and chitosan are heated to 128 DEG C in (4) by step, the one side of composite porous 1 and/or composite porous 2 is sprayed to after melting, and by staggered relatively for this face, thus by composite porous 1 and composite porous 2 adhesion-molded, obtained skeletal system repair materials.
beneficial effect
Compared with prior art, the present invention has following beneficial effect:
First, skeletal system repair materials rigidity prepared by the present invention, toughness all can reach the standard of skeleton, are applicable to the repair and reconstruction being applied to skeleton;
Secondly, skeletal system repair materials prepared by the present invention can bear the daily physiological stress stood;
Finally, the skeletal system repair materials prepared of the present invention is without any side effects.
Detailed description of the invention
Embodiment 1:
A kind of skeletal system repair materials, makes by mass fraction proportioning primarily of following raw material: collagen protein 15 parts, glycosaminoglycan 12 parts, hyaluronic acid 8 parts, 3 parts, calcium phosphate, magnesium phosphate 2 parts, chitosan 6 parts, Fibronectin 2 parts, CK8 part, distilled water 35 parts.
A preparation method for skeletal system repair materials, comprises following steps:
(1) collagen protein, Fibronectin and calcium phosphate are dissolved in distilled water, are heated to 28 DEG C and make slurry 1; Slurry 1 is deposited in a mold, is cooled to the temperature that slurry 1 is converted into multiple crystal or granule;
(2) by the crystal of acquisition in step (1) or heating particles to 80 DEG C, obtain composite porous 1, from mould, take out material;
(3) keratin, hyaluronic acid and magnesium phosphate are dissolved in distilled water, are heated to 28 DEG C and make slurry 2; Be deposited on after in mould until slurry 2, be cooled to-20 DEG C, after being all converted into multiple crystal or granule to slurry 2, be warming up to 40 DEG C with the speed of 10 DEG C/s, obtain composite porous 2, from mould, take out material;
(4) glycosaminoglycan and chitosan are heated to 95 DEG C, the one side of composite porous 1 and/or composite porous 2 is sprayed to after melting, and by staggered relatively for this face, thus by composite porous 1 and composite porous 2 adhesion-molded, obtained skeletal system repair materials.
Embodiment 2:
A kind of skeletal system repair materials, makes by mass fraction proportioning primarily of following raw material: collagen protein 35 parts, glycosaminoglycan 28 parts, hyaluronic acid 21 parts, 16 parts, calcium phosphate, magnesium phosphate 12 parts, chitosan 21 parts, Fibronectin 12 parts, keratin 23 parts, distilled water 60 parts.
A preparation method for skeletal system repair materials, comprises following steps:
(1) collagen protein, Fibronectin and calcium phosphate are dissolved in distilled water, are heated to 32 DEG C and make slurry 1; Slurry 1 is deposited in a mold, is cooled to the temperature that slurry 1 is converted into multiple crystal or granule;
(2) by the crystal of acquisition in step (1) or heating particles to 86 DEG C, obtain composite porous 1, from mould, take out material;
(3) keratin, hyaluronic acid and magnesium phosphate are dissolved in distilled water, are heated to 31 DEG C and make slurry 2; Be deposited on after in mould until slurry 2, be cooled to-18 DEG C, after being all converted into multiple crystal or granule to slurry 2, be warming up to 62 DEG C with the speed of 14 DEG C/s, obtain composite porous 2, from mould, take out material;
(4) glycosaminoglycan and chitosan are heated to 128 DEG C, the one side of composite porous 1 and/or composite porous 2 is sprayed to after melting, and by staggered relatively for this face, thus by composite porous 1 and composite porous 2 adhesion-molded, obtained skeletal system repair materials.
Embodiment 3:
A kind of skeletal system repair materials, makes by mass fraction proportioning primarily of following raw material: collagen protein 45 parts, glycosaminoglycan 30 parts, hyaluronic acid 25 parts, 20 parts, calcium phosphate, magnesium phosphate 16 parts, chitosan 26 parts, Fibronectin 15 parts, keratin 27 parts, distilled water 65 parts.
A preparation method for skeletal system repair materials, comprises following steps:
(1) collagen protein, Fibronectin and calcium phosphate are dissolved in distilled water, are heated to 37 DEG C and make slurry 1; Slurry 1 is deposited in a mold, is cooled to the temperature that slurry 1 is converted into multiple crystal or granule;
(2) by the crystal of acquisition in step (1) or heating particles to 90 DEG C, obtain composite porous 1, from mould, take out material;
(3) keratin, hyaluronic acid and magnesium phosphate are dissolved in distilled water, are heated to 37 DEG C and make slurry 2; Be deposited on after in mould until slurry 2, be cooled to 0 DEG C, after being all converted into multiple crystal or granule to slurry 2, be warming up to 65 DEG C with the speed of 15 DEG C/s, obtain composite porous 2, from mould, take out material;
(4) glycosaminoglycan and chitosan are heated to 132 DEG C, the one side of composite porous 1 and/or composite porous 2 is sprayed to after melting, and by staggered relatively for this face, thus by composite porous 1 and composite porous 2 adhesion-molded, obtained skeletal system repair materials.
Detect the skeletal system repair materials that embodiment 1 ~ 3 prepares, result is as shown in the table:
| Stress/kPa | Water content/% | The content of organic matter/% | Inorganic salt content % | |
| Embodiment 1 | 200 | 23 | 39 | 61 |
| Embodiment 2 | 254 | 22 | 40 | 60 |
| Embodiment 3 | 231 | 24 | 41 | 59 |
Claims (7)
1. a skeletal system repair materials, it is characterized in that, make by mass fraction proportioning primarily of following raw material: collagen protein 15 ~ 45 parts, glycosaminoglycan 12 ~ 30 parts, hyaluronic acid 8 ~ 25 parts, 3 ~ 20 parts, calcium phosphate, magnesium phosphate 2 ~ 16 parts, chitosan 6 ~ 26 parts, Fibronectin 2 ~ 15 parts, CK8 ~ 27 part, distilled water 35 ~ 65 parts.
2. a kind of skeletal system repair materials according to claim 1, it is characterized in that, make by mass fraction proportioning primarily of following raw material: collagen protein 35 parts, glycosaminoglycan 28 parts, hyaluronic acid 21 parts, 16 parts, calcium phosphate, magnesium phosphate 12 parts, chitosan 21 parts, Fibronectin 12 parts, keratin 23 parts, distilled water 60 parts.
3. the preparation method of a kind of skeletal system repair materials according to claim 1, is characterized in that, comprise following steps:
(1) collagen protein, Fibronectin and calcium phosphate are dissolved in distilled water, are heated to 28 ~ 37 DEG C and make slurry 1; Slurry 1 is deposited in a mold, is cooled to the temperature that slurry 1 is converted into multiple crystal or granule;
(2) by the crystal of acquisition in step (1) or heating particles to 80 ~ 90 DEG C, obtain composite porous 1, from mould, take out material;
(3) keratin, hyaluronic acid and magnesium phosphate are dissolved in distilled water, are heated to 28 ~ 37 DEG C and make slurry 2; Be deposited on after in mould until slurry 2, be cooled to-20 ~ 0 DEG C, after being all converted into multiple crystal or granule to slurry 2, be warming up to 40 ~ 65 DEG C with the speed of 10 ~ 15 DEG C/s, obtain composite porous 2, from mould, take out material;
(4) glycosaminoglycan and chitosan are heated to 95 ~ 132 DEG C, the one side of composite porous 1 and/or composite porous 2 is sprayed to after melting, and by staggered relatively for this face, thus by composite porous 1 and composite porous 2 adhesion-molded, obtained skeletal system repair materials.
4. the preparation method of a kind of skeletal system repair materials according to claim 3, it is characterized in that, collagen protein, Fibronectin and calcium phosphate are dissolved in distilled water in (1) by step, are heated to 32 DEG C and make slurry 1; Slurry 1 is deposited in a mold, is cooled to the temperature that slurry 1 is converted into multiple crystal or granule.
5. the preparation method of a kind of skeletal system repair materials according to claim 3, is characterized in that, in step (2), by the crystal that obtains in step (1) or heating particles to 86 DEG C, obtains composite porous 1, from mould, takes out material.
6. the preparation method of a kind of skeletal system repair materials according to claim 3, it is characterized in that, keratin, hyaluronic acid and magnesium phosphate are dissolved in distilled water in (3) by step, are heated to 31 DEG C and make slurry 2; Be deposited on after in mould until slurry 2, be cooled to-18 DEG C, after being all converted into multiple crystal or granule to slurry 2, be warming up to 62 DEG C with the speed of 14 DEG C/s, obtain composite porous 2, from mould, take out material.
7. the preparation method of a kind of skeletal system repair materials according to claim 3, it is characterized in that, glycosaminoglycan and chitosan are heated to 128 DEG C in (4) by step, the one side of composite porous 1 and/or composite porous 2 is sprayed to after melting, and by staggered relatively for this face, thus by composite porous 1 and composite porous 2 adhesion-molded, obtained skeletal system repair materials.
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| WO2010024549A2 (en) * | 2008-08-29 | 2010-03-04 | 한스바이오메드 주식회사 | Bone filler containing a sustained-release therapeutic agent for osteoporosis |
| CN101856509A (en) * | 2010-06-18 | 2010-10-13 | 华东理工大学 | Calcium magnesium injectable bone cement and preparation method and application thereof |
| WO2011058443A1 (en) * | 2009-11-13 | 2011-05-19 | Barralet Jake E | Method and device to induce collagen biomineralisation |
| CN102784014A (en) * | 2012-08-14 | 2012-11-21 | 中国科学院深圳先进技术研究院 | Porotic bone scaffold and preparation method thereof |
| CN103800944A (en) * | 2014-02-24 | 2014-05-21 | 李陵江 | Bone-grafting filling material and preparation method thereof |
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- 2015-06-24 CN CN201510352125.5A patent/CN104984388A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101084025A (en) * | 2004-09-14 | 2007-12-05 | 新加坡科技研究局 | Porous biomaterial-filler composite and a method for making the same |
| WO2010024549A2 (en) * | 2008-08-29 | 2010-03-04 | 한스바이오메드 주식회사 | Bone filler containing a sustained-release therapeutic agent for osteoporosis |
| WO2011058443A1 (en) * | 2009-11-13 | 2011-05-19 | Barralet Jake E | Method and device to induce collagen biomineralisation |
| CN101856509A (en) * | 2010-06-18 | 2010-10-13 | 华东理工大学 | Calcium magnesium injectable bone cement and preparation method and application thereof |
| CN102784014A (en) * | 2012-08-14 | 2012-11-21 | 中国科学院深圳先进技术研究院 | Porotic bone scaffold and preparation method thereof |
| CN103800944A (en) * | 2014-02-24 | 2014-05-21 | 李陵江 | Bone-grafting filling material and preparation method thereof |
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