CN104975105B - SNP marker, primer pair and its application for mouse metallothionein-Ⅰ identification - Google Patents
SNP marker, primer pair and its application for mouse metallothionein-Ⅰ identification Download PDFInfo
- Publication number
- CN104975105B CN104975105B CN201510469373.8A CN201510469373A CN104975105B CN 104975105 B CN104975105 B CN 104975105B CN 201510469373 A CN201510469373 A CN 201510469373A CN 104975105 B CN104975105 B CN 104975105B
- Authority
- CN
- China
- Prior art keywords
- seq
- primer pair
- sequence
- classified
- snp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention discloses a kind of SNP marker, primer pair and its application for mouse metallothionein-Ⅰ identification.Utilize 7 SNP sites sifted out：SNP1, SNP2, SNP3, SNP4, SNP5, SNP6 and SNP7, design 7 pairs of primers, sequence such as SEQ ID NO:1~SEQ ID NO:Shown in 14；Each pair primer can expand the genome sequence of mouse metallothionein-Ⅰ, sequence such as SEQ ID NO under similarity condition:15~SEQ ID NO:Shown in 21.And the sequence that each pair primer amplification goes out all contains a specific SNP marker.The application of SNP marker and primer pair can reach quick primary dcreening operation and reduce the purpose of testing cost, so as to promote inbred mouse genetic quality monitoring standardized process.
Description
Technical field
Full-length genome analysis of biological information technical advantage is the present invention relates to the use of, and is tested with PCR amplification and sanger sequencings
SNP marker is demonstrate,proved, and is detected the application of identification to production unit mouse metallothionein-Ⅰ using mass-spectrometric technique and SNP marker, especially
It is related to SNP marker, primer pair and its application for mouse metallothionein-Ⅰ identification.
Background technology
At present the country Bio-pharmaceutical Industry grow rapidly, experimental animal as Bio-pharmaceutical Industry important support condition and
The important materials of life science, its quality are directly related to the reliability and authenticity of experimental result.The something lost of experimental animal
Mass transfer amount is one of an important factor for Quality of Experimental Animals is fine or not, and the experimental animal only stablized using hereditary capacity just can guarantee that
Biomedical research result is comparable and the reliability of experimental result.Therefore, Quality of Experimental Animals standardizes, especially hereditary
The standardization of quality will directly affect the R ＆ D Level of biological medicine.But due to lacking unified, standardization quality of heredity control
Standard processed, the quality of heredity monitoring of experimental animal also become the problem of more prominent in its production application gradually.With regard to present circumstances
Speech, effective genetic marker that examination is widely recognized as to both at home and abroad are to realize having on the way for laboratory animal genetic quality-monitoring technological break-through
One of footpath.
At present, laboratory animal genetic monitoring in China's is main carries out national standard (GB 14923-2010), mainly takes form
Learn (coat color gene method of testing), Quantitative Genetics (mandibular determination method), immune labeled DNA test (epidermization) with
And Biochemical markers detection method etc..These methods are each advantageous, and in the breeding production of experimental animal and daily monitoring pipe
Science and engineering still has vital use value in making, but with the rapid development of Laboratory Animal Science and molecular biology, tradition
Detection method include national standard Biochemical markers detection method, epidermization and the immunological detection pushed cannot be complete
Adapt to the genetic monitoring work of a variety of strain experimental animals.
With DNA fingerprint technology, DNArandom amplified polymorphic DNA (RAPD), microsatellite and single nucleotide polymorphism (SNP) etc. one
The discovery of Series Molecules genetic marker, microsatellite and SNP, which show one's talent, has been widely used in the Genetic Detection of experimental animal.
SNP marker is the third generation DNA molecular marker after biochemical marker and RAPD genetic markers.Have the advantages that following notable：
1) those some homogenic types being difficult to discriminate between in itself and homologous strain can be differentiated relative to biochemical process and immunological method；2)
SNP detection method important advantage more convenient than biochemical process is to be not required to put to death animal, and all gene purity are next instead of in its production
It is preceding to be just guaranteed；3) SNP detection methods can reach high throughput, low cost and automation, disclosure satisfy that large-scale detection needs
Ask.Therefore, experimental animal SNP marker possesses larger research potential quality and application value at home.
2002, international mouse gene order-checking alliance completed the full survey of the laboratory strain C57BL/6J (B6) of classics
Sequence.The same year, Celera companies also complete the complete sequence sketch of the other three inbred strais.Due to SNP and microsatellite substantial amounts,
High-throughout Genotyping can reduce cost, therefore famous experimental animal company is used in both SNP and carries out Genetic Detection.For losing
Pass quality testing and do not have unified standard, its Sites Combination and quantity are the actual conditions decisions of as needed and each company
's.Well-known experimental animal supplier in the world, Jackson Laboratory (JAX), there is provided 2000 SNP assess chip, and
Genetic Detection is carried out to tens kinds of mouse metallothionein-Ⅰs using 28 SNP sites.
The content of the invention
The shortcomings that in order to overcome the prior art and deficiency, are used for mouse metallothionein-Ⅰ it is an object of the invention to provide one kind and reflect
Fixed SNP marker.It is at most of high cost to detect SNP site, and the country commonly uses mouse species about 10 to plant, therefore, the present invention
It is intended to sift out a few SNP that can be separated the identification country and commonly use mouse metallothionein-Ⅰ using existing inbred mouse snp database
Mark, to achieve the purpose that quick primary dcreening operation and reduce testing cost.
Another object of the present invention is to provide the primer pair for mouse metallothionein-Ⅰ identification.Totally 7 pairs of the primer pair of offer；
And the sequence that each pair primer amplification goes out all contains a specific SNP marker.The application of primer can reach quick primary dcreening operation and reduction
The purpose of testing cost, so as to promote inbred mouse genetic quality monitoring standardized process.
Another object of the present invention is to provide the application of the SNP marker.
It is still another object of the present invention to provide the application of the primer pair.
The purpose of the present invention is achieved through the following technical solutions：
The present invention carries out examination to NCBI mouse metallothionein-Ⅰs snp database first, sifts out 7 SNP sites, and design and draw
Thing, with 7 pairs of primer PCRs of design amplification 129, C3H, BALB, C57BL, CBA, DBA, FVB and ICR this 8 mouse metallothionein-Ⅰs
Genome sequence, then sanger sequencings, obtain sequence dna fragment, and verify 7 SNP sites, finally using mass spectrographic method
Domestic common this 7 sites of mouse metallothionein-Ⅰ are detected, determine the applicability of 7 SNP.
7 SNP sites are SNP1, SNP2, SNP3, SNP4, SNP5, SNP6 and SNP7.
The SNP1 represents that the SNP site is located at SEQ ID NO：The 91st of nucleotide sequence shown in 15, and
Nucleotides sequence is classified as degeneracy base K (G/T) at the position；
The SNP2 represents that the SNP site is located at SEQ ID NO：The 196th of nucleotide sequence shown in 16, and
Nucleotides sequence is classified as degeneracy base M (A/C) at the position；
The SNP3 represents that the SNP site is located at SEQ ID NO：The 112nd of nucleotide sequence shown in 17, and
Nucleotides sequence is classified as degeneracy base R (A/G) at the position；
The SNP4 represents that the SNP site is located at SEQ ID NO：The 294th of nucleotide sequence shown in 18, and
Nucleotides sequence is classified as degeneracy base Y (C/T) at the position；
The SNP5 represents that the SNP site is located at SEQ ID NO：The 188th of nucleotide sequence shown in 19, and
Nucleotides sequence is classified as degeneracy base R (A/G) at the position；
The SNP6 represents that the SNP site is located at SEQ ID NO：The 244th of nucleotide sequence shown in 20, and
Nucleotides sequence is classified as degeneracy base Y (C/T) at the position；
The SNP7 represents that the SNP site is located at SEQ ID NO：The 217th of nucleotide sequence shown in 21, and
Nucleotides sequence is classified as degeneracy base Y (C/T) at the position；
Totally 7 pairs of the primer pair, sequence such as SEQ ID NO：1~SEQ ID NO：Shown in 14.
The DNA fragmentation, sequence such as SEQ ID NO：15~SEQ ID NO：Shown in 21.
7 SNP sites can be applied to the identification of domestic common mouse metallothionein-Ⅰ.
The primer pair can be applied to the identification of domestic common mouse metallothionein-Ⅰ.
Application of 7 SNP sites in reagent preparation box or detection method；The kit or detection method
Purposes is identified for mouse metallothionein-Ⅰ or species identification.
Application of the primer pair in reagent preparation box or detection method；The purposes of the kit or detection method
For mouse metallothionein-Ⅰ identification or species identification.
Application of the DNA fragmentation in auxiliary carries out mouse metallothionein-Ⅰ or species identification.
The present invention is had the following advantages and effect relative to the prior art：
(1) present invention develops a few using biology information technology advantage can be used for domestic common inbred mouse mirror
Determine SNP marker, the application of SNP marker, which can substantially reduce the cost of inbred mouse identification and simplify, to be operated, and promotes inbred strais small
Mouse genetic quality monitoring standardizes and process.
(2) 7 pairs of primer pairs provided by the invention, the sequence that each pair primer amplification goes out all contain a specific SNP marker；
The application of primer can reach quick primary dcreening operation and reduce the purpose of testing cost, so as to promote inbred mouse genetic quality monitoring mark
Quasi-ization process.
Embodiment
With reference to embodiment, the present invention is described in further detail, but the implementation of the present invention is not limited to this.
Embodiment 1
1st, experimental animal sample
Mouse metallothionein-Ⅰ：129、C3H、BALB、C57BL、CBA、DBA、FVB、ICR.
2nd, animal tissue source：Experimental Animals Supervising Station, Guangdong Prov., Ji'nan University and Nanfang Medical Univ etc..
3rd, the screening of target SNP
Utilize https://www.ncbi.nlm.nih.gov/snp/Term=mouse database combination pertinent literature reports
Road, sifts out 7 SNP sites.It is shown in Table 1 in detail.
1 SNP marker of table and the genotype in each mouse metallothionein-Ⅰ
| SNP ID | Chr | SEQ ID | Position | 129 | C3H | BALB/cJ | C57BL/6 | C57BL/10 | CBA/C | CBA/J | DBA/1 | FVB | ICR |
| SNP1 | 7 | NO:15 | 91 | G | G | G | T | T | G | G | G | G | G |
| SNP2 | 11 | NO:16 | 196 | A | C | C | A | C | A | A | A | A | C |
| SNP3 | 15 | NO:17 | 112 | A | A | G | G | G | A | A | A | G | G |
| SNP4 | X | NO:18 | 294 | C | T | T | T | T | T | C | C | C | T |
| SNP5 | 17 | NO:19 | 188 | A | G | A | A | A | G | G | G | G | G |
| SNP6 | 3 | NO:20 | 244 | C | C | T | T | T | C | C | C | T | C |
| SNP7 | 1 | NO:21 | 217 | C | C | T | T | T | T | T | C | C | C |
4th, design of primers
After the completion of target SNP screenings, the fragment of each 500bp of interception SNP upstream and downstream, utilizes primer-design software Priemer
5.0 carry out design of primers.Ensure that primer amplification fragment contains target SNP, primer length is between 18~22bp, annealing temperature 55
℃.7 pairs of primers are designed altogether, and primer sequence such as table 2, sequence number is SEQ ID NO in sequence table:1~SEQ ID NO:14.Institute
Commission Beijing Huada gene company synthesizes after the completion of having design of primers.
2 primer sequence information of table
5th, target fragment PCR amplification
With 7 pairs of primer amplifications 129, C3H, BALB, C57BL, CBA, DBA, FVB and ICR gene order of design.
The Animal genome DNA extraction examinations that extracting genome DNA is manufactured with reference to Pu Boxin bio tech ltd
The specification of agent box (centrifugal column method) is operated.The primer of synthesis carries out of short duration centrifugation above, adds aseptic deionized water and fills
Divide and be dissolved into concentration as 10 μM, be stored at room temperature 30 minutes, 4 DEG C of preservations.
PCR reactions, PCR reaction systems (20 μ L) such as table 3 are carried out using the genomic DNA of each animal specimen as template.
3 PRC reaction systems of table
| Title | Concentration | Volume |
| Genomic DNA | 10ng/μL | 5μL |
| Sense primer | 10μM | 0.8μL |
| Anti-sense primer | 10μM | 0.8μL |
| PCR kit(2×) | 10μL | |
| ddH2O | 3.4μL |
Of short duration centrifugation, is placed to PCR instrument after mixing, and setting cyclic program is：
Above-mentioned PCR reaction products carry out 1.0% agarose gel electrophoresis identification.
Weigh 0.5g agaroses and be added in 50mL TAE reaction solutions and dissolve by heating, when temperature is down to 60 DEG C, add bromination
3 μ L of second ingot analog are mixed, and are poured into and are inserted with the electrophoresis tank of 2mm combs, wait rear use to be solidified.Add 5 μ L PCR products per hole,
Using 121V constant pressures, observed after electrophoresis 15min under ultraviolet transilluminator as a result, target stripe is all single bright.
All successful PCR products of amplification directly carry out DNA sequencing, whole examining orders are by Beijing Hua Da with 4 DEG C of preservations
Complete in sequencing portion of genome company.
6th, sequencing sequence is analyzed
6.1 sequencing sequences splice
Since the sequence initiating terminal sequencing quality of Sanger sequencings is relatively low, accuracy is relatively poor, using forward primer and
Reverse primer is sequenced respectively, and according to sequencing peak figure, sequence is complementary to one another, and is spliced into complete sequencing sequence.8 inbred strais
General sequence information is shown in sequence table SEQ ID NO:15~SEQ ID NO:Shown in 21.
6.2PCR Sequence Identifications and SNP site
The sequence that sequencing is obtained and sequence alignment in protogene group, pass through the Identity (homology) with original series
Whether the sequence to determine to be expanded with the primer PCR is target sequence, 7 pairs of primer PCR product sequencing results and original base
Because group sequence alignment homology is all more than 99%, sequence all compares back corresponding genome sequence well, and covers
Purpose SNP site, is shown in sequence SEQ ID NO:15~SEQ ID NO:21.Showing the primer of design can accurately expand really
Increase and target area and cover target SNP site.
7th, parting is carried out to 7 SNP sites of different inbred strais using mass spectrometry method
It is single to Experimental Animals Supervising Station, Guangdong Prov., Ji'nan University and Nanfang Medical Univ etc. using mass spectrography MassArray
Mouse metallothionein-Ⅰ 129, C3H, BALB, C57BL, CBA, DBA, FVB, ICR of position collection carry out the parting of 7 SNP sites, as a result
It is shown in Table 4.PRELIMINARY RESULTS is substantially with expected consistent, but it is not expected results to have a few sample yet.
Genotype of 4 SNP marker of table in actual mouse metallothionein-Ⅰ
| SNP ID | Chr | SEQ ID | Position | 129 | C3H | BALB | C57BL | CBA | DBA | FVB | ICR |
| SNP1 | 7 | NO:15 | 91 | G | G | G | TG | G | G | G | G |
| SNP2 | 11 | NO:16 | 196 | A | C | C | A | A | A | A | C(CA) |
| SNP3 | 15 | NO:17 | 112 | A | A | G | G | A | A | G | G |
| SNP4 | X | NO:18 | 294 | C | T | T | T | C | C | C | T |
| SNP5 | 17 | NO:19 | 188 | A | G | A | A | G | G | G | G |
| SNP6 | 3 | NO:20 | 244 | C | C | T | T | C | C | T | C |
| SNP7 | 1 | NO:21 | 217 | C | C | T | T or C | T | C | C | C or T or CT |
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention and from above-described embodiment
Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (7)
- A kind of 1. application for the SNP marker of mouse metallothionein-Ⅰ identification in the identification of mouse metallothionein-Ⅰ, it is characterised in that institute The SNP marker stated is made of 7 SNP sites, is SNP1, SNP2, SNP3, SNP4, SNP5, SNP6 and SNP7；The SNP1 represents that the SNP site is located at SEQ ID NO：The 91st of nucleotide sequence shown in 15, and the position The place's of putting nucleotides sequence is classified as degeneracy base K；The SNP2 represents that the SNP site is located at SEQ ID NO：The 196th of nucleotide sequence shown in 16, and the position The place's of putting nucleotides sequence is classified as degeneracy base M；The SNP3 represents that the SNP site is located at SEQ ID NO：The 112nd of nucleotide sequence shown in 17, and the position The place's of putting nucleotides sequence is classified as degeneracy base R；The SNP4 represents that the SNP site is located at SEQ ID NO：The 294th of nucleotide sequence shown in 18, and the position The place's of putting nucleotides sequence is classified as degeneracy base Y；The SNP5 represents that the SNP site is located at SEQ ID NO：The 188th of nucleotide sequence shown in 19, and the position The place's of putting nucleotides sequence is classified as degeneracy base R；The SNP6 represents that the SNP site is located at SEQ ID NO：The 244th of nucleotide sequence shown in 20, and the position The place's of putting nucleotides sequence is classified as degeneracy base Y；The SNP7 represents that the SNP site is located at SEQ ID NO：The 217th of nucleotide sequence shown in 21, and the position The place's of putting nucleotides sequence is classified as degeneracy base Y；The mouse metallothionein-Ⅰ is at least one of 129, C3H, BALB, C57BL, CBA, DBA, FVB and ICR.
- 2. a kind of application for the SNP marker of mouse metallothionein-Ⅰ identification in reagent preparation box or detection method, its feature exists In：The kit or the purposes of detection method are mouse metallothionein-Ⅰ identification or species identification；The SNP marker is made of 7 SNP sites, is SNP1, SNP2, SNP3, SNP4, SNP5, SNP6 and SNP7；The SNP1 represents that the SNP site is located at SEQ ID NO：The 91st of nucleotide sequence shown in 15, and the position The place's of putting nucleotides sequence is classified as degeneracy base K；The SNP2 represents that the SNP site is located at SEQ ID NO：The 196th of nucleotide sequence shown in 16, and the position The place's of putting nucleotides sequence is classified as degeneracy base M；The SNP3 represents that the SNP site is located at SEQ ID NO：The 112nd of nucleotide sequence shown in 17, and the position The place's of putting nucleotides sequence is classified as degeneracy base R；The SNP4 represents that the SNP site is located at SEQ ID NO：The 294th of nucleotide sequence shown in 18, and the position The place's of putting nucleotides sequence is classified as degeneracy base Y；The SNP5 represents that the SNP site is located at SEQ ID NO：The 188th of nucleotide sequence shown in 19, and the position The place's of putting nucleotides sequence is classified as degeneracy base R；The SNP6 represents that the SNP site is located at SEQ ID NO：The 244th of nucleotide sequence shown in 20, and the position The place's of putting nucleotides sequence is classified as degeneracy base Y；The SNP7 represents that the SNP site is located at SEQ ID NO：The 217th of nucleotide sequence shown in 21, and the position The place's of putting nucleotides sequence is classified as degeneracy base Y；The mouse metallothionein-Ⅰ is at least one of 129, C3H, BALB, C57BL, CBA, DBA, FVB and ICR.
- A kind of 3. primer pair for mouse metallothionein-Ⅰ identification, it is characterised in that：Including primer pair SNP1F/R, primer pair SNP2F/R, primer pair SNP3F/R, primer pair SNP4F/R, primer pair SNP5F/R, primer pair SNP6F/R and primer pair SNP7F/ R；The sequence of primer pair SNP1F/R such as SEQ ID NO:1 and SEQ ID NO:Shown in 2；The sequence of primer pair SNP2F/R such as SEQ ID NO:3 and SEQ ID NO:Shown in 4；The sequence of primer pair SNP3F/R such as SEQ ID NO:5 and SEQ ID NO:Shown in 6；The sequence of primer pair SNP4F/R such as SEQ ID NO:7 and SEQ ID NO:Shown in 8；The sequence of primer pair SNP5F/R such as SEQ ID NO:9 and SEQ ID NO:Shown in 10；The sequence of primer pair SNP6F/R such as SEQ ID NO:11 and SEQ ID NO:Shown in 12；The sequence of primer pair SNP7F/R such as SEQ ID NO:13 and SEQ ID NO:Shown in 14；The mouse metallothionein-Ⅰ is at least one of 129, C3H, BALB, C57BL, CBA, DBA, FVB and ICR.
- 4. the combination of the DNA sequence dna obtained using the primer pair amplifies described in claim 3, it is characterised in that：The DNA sequences Row such as SEQ ID NO:15~SEQ ID NO:Shown in 21.
- 5. application of the primer pair in the identification of mouse metallothionein-Ⅰ described in claim 3.
- 6. application of the primer pair in reagent preparation box or detection method described in claim 3, it is characterised in that：The examination The purposes of agent box or detection method is identified for mouse metallothionein-Ⅰ or species identification.
- 7. application of the combination of the DNA sequence dna described in claim 4 in auxiliary carries out mouse metallothionein-Ⅰ or species identification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510469373.8A CN104975105B (en) | 2015-07-31 | 2015-07-31 | SNP marker, primer pair and its application for mouse metallothionein-Ⅰ identification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510469373.8A CN104975105B (en) | 2015-07-31 | 2015-07-31 | SNP marker, primer pair and its application for mouse metallothionein-Ⅰ identification |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104975105A CN104975105A (en) | 2015-10-14 |
| CN104975105B true CN104975105B (en) | 2018-04-13 |
Family
ID=54272067
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510469373.8A Expired - Fee Related CN104975105B (en) | 2015-07-31 | 2015-07-31 | SNP marker, primer pair and its application for mouse metallothionein-Ⅰ identification |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104975105B (en) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105671040A (en) * | 2016-03-18 | 2016-06-15 | 中国人民解放军第二军医大学 | Naked mole rat MHC gene SNP locus and application thereof |
| CN106947825B (en) * | 2017-04-25 | 2020-04-28 | 华南理工大学 | SNP marker for origin (subgroup) identification of cynomolgus monkey and application thereof |
| CN108588236B (en) * | 2018-05-17 | 2021-02-26 | 江苏集萃药康生物科技股份有限公司 | SNP rapid detection method for monitoring genetic quality of inbred line, SNP locus and primer thereof |
| CN109022592B (en) * | 2018-08-27 | 2020-11-24 | 华南理工大学 | SNP marker for identifying four common strains of rats and application thereof |
| CN109609659B (en) * | 2018-12-21 | 2021-09-28 | 江苏集萃药康生物科技股份有限公司 | SNP loci for monitoring genetic quality of CBA/CaJ inbred line mice, primer combination and application thereof |
| CN109694914B (en) * | 2018-12-28 | 2022-06-03 | 江苏集萃药康生物科技股份有限公司 | Method for monitoring genetic quality of DBA inbred line mice, primer group and application thereof |
| CN110452969B (en) * | 2019-07-18 | 2020-09-04 | 苏州西山生物技术有限公司 | Rat genetic quality monitoring SNP marker typing method and kit based on KASP |
| CN110484627B (en) * | 2019-07-30 | 2023-05-02 | 江苏集萃药康生物科技股份有限公司 | Method for monitoring genetic quality of A/J inbred line mice, primer set and application thereof |
| CN110305974B (en) * | 2019-08-08 | 2023-06-23 | 广东省实验动物监测所 | PCR analysis primer for distinguishing common mouse inbred lines based on detection of five SNP loci and analysis method thereof |
| CN110358847B (en) * | 2019-09-03 | 2021-03-05 | 江苏集萃药康生物科技股份有限公司 | SNP loci for monitoring genetic quality of BALB/cJ inbred line mice, primer combination and application thereof |
| CN114317767A (en) * | 2021-12-21 | 2022-04-12 | 斯贝福（北京）生物技术有限公司 | Primer, kit and method for distinguishing C57BL/6 subline mouse |
| CN114507744B (en) * | 2022-04-19 | 2022-07-12 | 斯贝福（北京）生物技术有限公司 | Application of SNP (Single nucleotide polymorphism) marker in inbred line mouse strain identification and primer sequence |
| CN116004842B (en) * | 2022-07-21 | 2023-08-18 | 斯贝福（北京）生物技术有限公司 | Application of SNP marker in inbred line rat genetic quality detection and/or inbred line rat strain identification, and primer sequence |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102586457A (en) * | 2012-03-14 | 2012-07-18 | 东华大学 | Single nucleotide polymorphism (SNP) classification method for identifying inbred line mice |
| CN103436603A (en) * | 2013-07-15 | 2013-12-11 | 东华大学 | PCR method for identification of genotypes of offspring mice |
-
2015
- 2015-07-31 CN CN201510469373.8A patent/CN104975105B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102586457A (en) * | 2012-03-14 | 2012-07-18 | 东华大学 | Single nucleotide polymorphism (SNP) classification method for identifying inbred line mice |
| CN103436603A (en) * | 2013-07-15 | 2013-12-11 | 东华大学 | PCR method for identification of genotypes of offspring mice |
Non-Patent Citations (3)
| Title |
|---|
| Development of SNP markers for C57BL/6N-derived mouse inbred strains;Kazuyuki Mekada等;《Exp. Anim.》;20141023;第64卷(第1期);摘要部分，"Materials and Methods"部分，"Results"部分 * |
| 单管双向等位基因专一性扩增方法在近交系小鼠遗传检测中的应用;胡培丽等;《中国实验动物学报》;20061231;第14卷(第4期);第1.1-1.6部分，第2.4部分 * |
| 基于PCR-LDR平台的近交系小鼠遗传质量快速检测方法;谢雯等;《中国实验动物学报》;20120831;第20卷(第4期);第1.1-1.4部分，第2.3部分 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104975105A (en) | 2015-10-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104975105B (en) | SNP marker, primer pair and its application for mouse metallothionein-Ⅰ identification | |
| CN105385755A (en) | Method for conducting SNP-haplotype analysis by means of multiplex PCR technology | |
| CN113832243B (en) | Core SNP marker for tea tree variety identification based on KASP technology development | |
| CN106282394A (en) | The method of high throughput testing Semen Maydis south rust resistance gene type and test kit thereof | |
| WO2022134451A1 (en) | Snp molecular marker for identification of sex of osteoglossum bicirrhosum, and use thereof | |
| CN108424958A (en) | A kind of relevant SNP marker of Larimichthys crocea genetic sex and its primer and application | |
| CN108103208A (en) | A kind of SNP marker for influencing sheep Fecundity Trait and its application | |
| CN104862312A (en) | Primer pair for screening mammalian experiment animal SNP (Single Nucleotide Polymorphism) markers and application thereof | |
| Arbeiter et al. | Identification and validation of novel EST-SSR markers in olives | |
| CN103898226B (en) | A kind of plastosome SNP fluorescence labeling composite amplification test kit and application thereof | |
| CN110846429A (en) | Corn whole genome InDel chip and application thereof | |
| CN110622920A (en) | Breeding method of pure black and hairy pig breeds | |
| KR20180077873A (en) | SNP markers for selection of marker-assisted backcross in watermelon | |
| CN110541041B (en) | SNP marker related to Chinese domestic horse dwarf trait and application thereof | |
| CN107858447A (en) | For identifying single nucleotide polymorphism site, primer pair, kit and the application of peach blossom single-lobe/polyphyll character | |
| CN107988385B (en) | Method for detecting marker of PLAG1 gene Indel of beef cattle and special kit thereof | |
| CN106521004A (en) | Indel marker in linkage with carrot genic male sterility gene and application of Indel marker | |
| CN116837112B (en) | SNP molecular marker related to yak growth traits and application thereof | |
| CN117965781A (en) | Peanut 40K liquid-phase SNP chip 'PeanutGBTS K' and application thereof | |
| CN117106967A (en) | Functional KASP molecular marker of rice blast resistance gene and application thereof | |
| CN102732514B (en) | Identification method for chemotactic factor acceptor 9 gene used as molecular marker for bovine excellent superovulation trait and application of same | |
| CN105441569B (en) | A kind of detection method and kit of yak FOXO3 gene mononucleotide polymorphisms | |
| CN115679012B (en) | Chilli whole genome SNP-Panel and application thereof | |
| CN106755422B (en) | Detection method of MEG3 gene SNP related to cattle growth traits and application thereof | |
| CN109852723A (en) | A kind of SNP marker and its application with cowpea pod color gene close linkage |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180413 Termination date: 20200731 |