CN104974531A - Method for preparing antibacterial and antioxidant composite film by using scale - Google Patents
Method for preparing antibacterial and antioxidant composite film by using scale Download PDFInfo
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- CN104974531A CN104974531A CN201510399455.XA CN201510399455A CN104974531A CN 104974531 A CN104974531 A CN 104974531A CN 201510399455 A CN201510399455 A CN 201510399455A CN 104974531 A CN104974531 A CN 104974531A
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Abstract
The present invention discloses a method for preparing an antibacterial and antioxidant composite film by using scale. According to the method, scale gelatin is adopted as a substrate, a tea polyphenol-Zn complex is adopted as an antibacterial agent, the slow antibacterial agent release purpose is achieved by using cyclodextrin, and scale gelatin preparing, tea polyphenol-Zn complex synthesis, dissolution, homogenization , degassing, scraping to form film, drying and other steps are performed to prepare the antibacterial and antioxidant composite film.
Description
Technical field
The invention belongs to food processing field, relate to the production method of packing film, particularly relate to a kind of method utilizing fish scale to prepare antibacterial anti-oxidant composite membrane.
Background technology
In recent years, the environmental problem brought along with Plastic Packaging Materials becomes increasingly conspicuous, and people thirst for finding a kind of environmentally friendly products substitution Plastic Packaging Materials day by day, and microbial film becomes the focus of food packaging applications research with its outstanding feature.Microbial film is as a kind of new packaging material, there is the advantages such as environmental protection, biological degradation, nontoxic, the quality that can improve food, simultaneously, in microbial film production process, add the materials such as antiseptic-germicide, antioxidant, pigment, flavour substances, nutrition-fortifying agent, spice, give its more function.Therefore, plyability microbial film becomes development trend now.
Fish scale accounts for 2.5% of fish body weight, and majority is dropped in the course of processing, and both contaminate environment, waste Biological resources.There are some researches show, fish scale is one of high quality raw material preparing gelatin, if reasonable development, will produce larger economic benefit.
Based on this, applicant, according to the present Research of microbial film and fish scale, take fish scale as raw material, by series of processes process, prepares a kind of antimicrobial compound film.
Summary of the invention
The object of the present invention is to provide a kind of method utilizing fish scale to prepare antibacterial anti-oxidant composite membrane.
For achieving the above object, technical scheme of the present invention is: fish scale gelatin: tea-polyphenol-Zn mixture: cyclodextrin: softening agent (sorbyl alcohol): stablizer (tween-80)=100 ~ 125 part: 1.5 ~ 3.125 parts: 0.75 ~ 1.25 part: 20 ~ 25 parts: 1.5 ~ 3.125 parts.
The present invention is a kind of method utilizing fish scale to prepare antibacterial anti-oxidant composite membrane, it prepares the gelatin in fish scale by certain technique, then film-forming soln is re-dubbed with cyclodextrin, antiseptic-germicide, stablizer and softening agent, after knifing, drying and moulding, obtain antibacterial, anti-oxidant composite membrane, concrete steps are as follows:
(1) preparation of fish scale gelatin:fish scale is dry, pulverizing, joins in 3 ~ 5% HCl solution according to solid-liquid ratio 1 g:15 ~ 25 mL by fish scale, in room temperature ultrasonic immersion 5 ~ 6 h, and decalcification; After process, fish scale washing is to pH 6.5 ~ 7.0, adds 3 ~ 5% NaOH solution, after ultrasonic immersion 8 ~ 10 h, remove noncollagen protein after fully draining according to solid-liquid ratio 1 g:15 ~ 25 mL in decalcification fish scale; After process fish scale is washed to pH 6.5 ~ 7.0, join in fish scale according to solid-liquid ratio 1 g:8 ~ 10 mL by distilled water, extract 8 ~ 10 h in 75 ~ 80 DEG C, filter, filter vacuum concentrates, and obtains fish scale gelatin in 65 ~ 75 DEG C of vacuum-dryings;
(2) preparation of tea-polyphenol-Zn antiseptic-germicide:tea-polyphenol is joined in distilled water according to solid-liquid ratio 1g:3 ~ 5mL, pH value of solution to 6.5 ~ 7.0 are adjusted by 1 mol/L NaOH solution, under 600 ~ 1000 rpm whipped states, 0.1 mol/L ZnCl solution of same volume is joined in tea-polyphenol solution under nitrogen protection, reaction 45 ~ 60 min, cross the freeze-drying of leaching precipitation, obtain tea-polyphenol-Zn mixture;
(3) preparation of antibacterial, anti-oxidant composite membrane film-forming soln:cyclodextrin is added in distilled water according to solid-liquid ratio 0.3 ~ 0.5g:100 mL, after room temperature 600 ~ 1000 rpm stirs 30 ~ 60 min, add tea-polyphenol-Zn mixture and stablizer according to 20 ~ 25% of cyclodextrin weight, continue stirring 45 ~ 60 min and obtain dextrin-tea-polyphenol-Zn mixture inclusion compound emulsion; According to solid-liquid ratio 1g:20 ~ 25 mL and 1g:100 ~ 120 mL, fish scale gelatin and softening agent are joined in emulsion, after stirring at room temperature 3 ~ 5 h, vacuum outgas, obtained film-forming soln;
(4) preparation of antibacterial, anti-oxidant composite membrane:be poured on equably by the film-forming soln modulated on polyvinyl chloride crystal slab and carry out striking film forming, in 40 ~ 50% times dryings of 25 ~ 45 DEG C of relative humidity after 2 ~ 3 days, namely demoulding obtains antibacterial, anti-oxidant composite membrane.
As improvement, fish scale described in step (1), its source for large yellow croaker (
pseudosciaena croce).
As improvement, the cyclodextrin in step (3) is alpha-cylodextrin or beta-cyclodextrin.
As improvement, the stablizer in step (3) is tween-80.
As improvement, described in step (4), softening agent is sorbyl alcohol.
Compared with prior art, the invention has the advantages that:
1, the present invention utilizes disposal from fishery product processing fish scale for raw material, both can significantly improve the added value of fishery products, can reduce again the pollution of aquatic products processing waste to environment, is a kind of environmentally friendly technology.
2, the antimicrobial compound film that prepared by the present invention contains tea-polyphenol-Zn mixture, the breeding of Salmonellas, Shigellae, streptococcus aureus and Hemolytic streptococcus etc. can be suppressed, can also stop by UV-induced food lipid peroxidation simultaneously, extend the shelf-lives of food, improve food quality.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment:
(1) preparation of fish scale gelatin:fish scale is dry, pulverize, according to solid-liquid ratio 1 g:20 mL by large yellow croaker (
pseudosciaena croce) fish scale joins in 3% HCl solution, in room temperature ultrasonic immersion 5 h, decalcification; After process, fish scale washing is to pH 7.0, adds 4% NaOH solution, after ultrasonic immersion 10 h, remove noncollagen protein after fully draining according to solid-liquid ratio 1 g:25 mL in decalcification fish scale; After process fish scale is washed to pH 7.0, join in fish scale according to solid-liquid ratio 1 g:10 mL by distilled water, extract 10 h in 78 DEG C, filter, filter vacuum concentrates, and obtains fish scale gelatin in 70 DEG C of vacuum-dryings.
(2) preparation of tea-polyphenol-Zn antiseptic-germicide:tea-polyphenol is joined in distilled water according to solid-liquid ratio 1 g: 4 mL; pH value of solution to 7.0 is adjusted by 1 mol/L NaOH solution; under 600 ~ 1000 rpm whipped states, 0.1 mol/L ZnCl solution of same volume is joined in tea-polyphenol solution under nitrogen protection; react 50 min; filter; get precipitation freeze-drying, obtain tea-polyphenol-Zn mixture.
(3) preparation of antibacterial, anti-oxidant composite membrane film-forming soln:alpha-cylodextrin is added in distilled water according to solid-liquid ratio 0.4g:100 mL, after room temperature 600 ~ 1000 rpm stirs 30 min, add tea-polyphenol-Zn mixture and stablizer (tween-80) according to 20% of cyclodextrin weight, continue stirring 45 min and obtain dextrin-tea-polyphenol-Zn mixture inclusion compound emulsion; According to solid-liquid ratio 1g:20 mL and 1g:120 mL, fish scale gelatin and softening agent (sorbyl alcohol) are joined in emulsion, after stirring at room temperature 4 h, vacuum outgas, obtained film-forming soln.
(4) preparation of antibacterial, anti-oxidant composite membrane:be poured on equably by the film-forming soln modulated on polyvinyl chloride crystal slab and carry out striking film forming, in the 45% time drying of 35 DEG C of relative humidity after 2 days, namely demoulding obtains antibacterial, anti-oxidant composite membrane.Composite membrane of the present invention contains fish scale gelatin: tea-polyphenol-Zn mixture: cyclodextrin: softening agent (sorbyl alcohol): stablizer (tween-80)=100 ~ 125 part: 1.5 ~ 3.125 parts: 0.75 ~ 1.25 part: 20 ~ 25 parts: 1.5 ~ 3.125 parts.
The feature that antimicrobial compound film tool is antibacterial, anti-oxidant activity is strong prepared by the present invention, achieves beyond thought composite antibacterial effect, can promote the use of at food packaging applications.
Finally, still need it is noted that what enumerate above is only a specific embodiment of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be had.All distortion that those of ordinary skill in the art can directly derive from content disclosed by the invention or associate, all should think protection scope of the present invention.
Claims (4)
1. the antibacterial anti-oxidant composite membrane of fish scale, is characterized in that: by weight containing 100 ~ 125 parts of fish scale gelatins, 1.5 ~ 3.125 parts of tea-polyphenol-Zn mixtures, 0.75 ~ 1.25 part of cyclodextrin, 20 ~ 25 parts of softening agent, 1.5 ~ 3.125 parts of stablizers.
2. the antibacterial anti-oxidant composite membrane of fish scale according to claim 1, is characterized in that: described softening agent is sorbyl alcohol; Described stablizer is tween-80.
3. the antibacterial anti-oxidant composite membrane of fish scale according to claim 1; it is characterized in that: described tea-polyphenol-Zn mixture is adopted and prepared with the following method: join in distilled water by tea-polyphenol according to solid-liquid ratio 1g:3 ~ 5mL; pH value of solution to 6.5 ~ 7.0 are adjusted by 1 mol/L NaOH solution; under 600 ~ 1000 rpm whipped states, 0.1 mol/L ZnCl solution of same volume is joined in tea-polyphenol solution under nitrogen protection; reaction 45 ~ 60 min; cross the freeze-drying of leaching precipitation, obtain tea-polyphenol-Zn mixture.
4. the purposes of the antibacterial anti-oxidant composite membrane of fish scale, is characterized in that: the antibacterial anti-oxidant composite membrane of this fish scale is for the preparation of the purposes of packaging material for food.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106465744A (en) * | 2016-09-27 | 2017-03-01 | 江南大学 | A kind of fresh-keeping film liquid fresh-keeping for fresh Tilapia Fillet and preparation method thereof |
| CN106751918A (en) * | 2016-11-23 | 2017-05-31 | 宁波大学 | A kind of preparation method of bamboo-leaves flavones fish scale gelatin edible freshness-keeping thin coat |
| CN107177044A (en) * | 2016-03-09 | 2017-09-19 | 深圳先进技术研究院 | A kind of collagen-Tea Polyphenols antibacterial film and preparation method thereof |
| CN107739515A (en) * | 2017-10-19 | 2018-02-27 | 张永华 | A kind of isinglass antibacterial oxidation-resistant film and preparation method thereof |
| CN108719850A (en) * | 2018-05-16 | 2018-11-02 | 大连工业大学 | Enhance the method for gel characteristic based on UVA induction kelp polyphenol oxidases |
| WO2021019454A1 (en) * | 2019-07-31 | 2021-02-04 | Università Degli Studi Di Sassari | Animal gelatins obtained from fish skin modified with cyclodextrins, process for the preparation thereof, films obtained therefrom and use thereof in the packaging field |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102558878A (en) * | 2012-01-04 | 2012-07-11 | 福州大学 | Green tea extract containing fishskin collagen antioxidation film and preparation method thereof |
| CN102702163A (en) * | 2012-06-01 | 2012-10-03 | 广西济康生物科技有限公司 | Method for preparing high-purity monomer epigallocatechin gallate from processed leftovers of tea leaves |
| CN104693811A (en) * | 2015-03-27 | 2015-06-10 | 四川大学 | Functional gelatin food packaging film and preparation method thereof |
-
2015
- 2015-07-09 CN CN201510399455.XA patent/CN104974531A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102558878A (en) * | 2012-01-04 | 2012-07-11 | 福州大学 | Green tea extract containing fishskin collagen antioxidation film and preparation method thereof |
| CN102702163A (en) * | 2012-06-01 | 2012-10-03 | 广西济康生物科技有限公司 | Method for preparing high-purity monomer epigallocatechin gallate from processed leftovers of tea leaves |
| CN104693811A (en) * | 2015-03-27 | 2015-06-10 | 四川大学 | Functional gelatin food packaging film and preparation method thereof |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107177044A (en) * | 2016-03-09 | 2017-09-19 | 深圳先进技术研究院 | A kind of collagen-Tea Polyphenols antibacterial film and preparation method thereof |
| CN106465744A (en) * | 2016-09-27 | 2017-03-01 | 江南大学 | A kind of fresh-keeping film liquid fresh-keeping for fresh Tilapia Fillet and preparation method thereof |
| CN106751918A (en) * | 2016-11-23 | 2017-05-31 | 宁波大学 | A kind of preparation method of bamboo-leaves flavones fish scale gelatin edible freshness-keeping thin coat |
| CN106751918B (en) * | 2016-11-23 | 2019-05-03 | 宁波大学 | A kind of preparation method of bamboo-leaves flavones fish scale gelatin edible freshness-keeping thin coat |
| CN107739515A (en) * | 2017-10-19 | 2018-02-27 | 张永华 | A kind of isinglass antibacterial oxidation-resistant film and preparation method thereof |
| CN108719850A (en) * | 2018-05-16 | 2018-11-02 | 大连工业大学 | Enhance the method for gel characteristic based on UVA induction kelp polyphenol oxidases |
| WO2021019454A1 (en) * | 2019-07-31 | 2021-02-04 | Università Degli Studi Di Sassari | Animal gelatins obtained from fish skin modified with cyclodextrins, process for the preparation thereof, films obtained therefrom and use thereof in the packaging field |
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Application publication date: 20151014 |