CN102786702A - Preparation method of controlled release antibacterial film - Google Patents
Preparation method of controlled release antibacterial film Download PDFInfo
- Publication number
- CN102786702A CN102786702A CN2012102488862A CN201210248886A CN102786702A CN 102786702 A CN102786702 A CN 102786702A CN 2012102488862 A CN2012102488862 A CN 2012102488862A CN 201210248886 A CN201210248886 A CN 201210248886A CN 102786702 A CN102786702 A CN 102786702A
- Authority
- CN
- China
- Prior art keywords
- preparation
- solution
- film
- diacetylmuramidase
- nanoparticle powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Medicinal Preparation (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
Abstract
The invention discloses a preparation method of a controlled release antibacterial film. The method includes the following steps of: (1) dissolving zein and a hydrophobic antibacterial agent into an alcoholic solution; (2) preparing a sodium caseinate aqueous solution; (3) adding the sodium caseinate aqueous solution into the solution obtained in step (1) under stirring; (4) subjecting the solution obtained in step (3) to rotary evaporation and centrifugation, then freeze drying the supernatant, thus obtaining nanoparticle powder; (5) dissolving a film-forming matrix in water, adding the nanoparticle powder and lysozyme to form a mixed solution; and (6) adding glycerin, and conducting film pouring, thus obtaining the film product. The method provided in the invention can simultaneously reduce initial microbial activity and inhibit microbial growth for a long term, and has the advantages of low instrument requirement, and controllable bacterium inhibiting intensity, thus having broad application prospects in food and medical products.
Description
Technical field
The present invention relates to a kind of preparation method of controlled release antibacterial film, raw material all adopts foodstuffs material, belongs to agricultural byproducts deep processing and food industrial technical field.
Background technology
To grow be the important factor that causes food spoilage to pathogenic microorganism in food-processing, the storage, is the crucial scientific and technological problem that needs to be resolved hurrily of field of food so guarantee food safety with prolonging the food shelf-lives.In food, add natural antimicrobial substance instead of chemical synthetic preservative and become trend.Thymol, N,O-Diacetylmuramidase and nisin are the natural antimicrobial substance that exists at occurring in nature, but because factors such as irritating smell, low, the effective antibacterial time weak point of solubleness, it receives the restriction of knowing clearly in Application in Food.Therefore this type of antiseptic-germicide need be loaded in the carrier.Antibacterial film based on natural protein is one type of carrier that reaches the ideal antimicrobial substance of food requirement.Because mikrobe is grown in food surfaces mostly, this nano material reduces the destruction to food substrate simultaneously at the strong bacteriostatic of imitating, and its certain stable and slow release effect can prolong antibacterial effect.
The antiseptic-germicide that directly adds waits until from film network and is discharged into behind the external world just and microbial process that rate of release is very fast; The antiseptic-germicide that adds after the embedding also need be discharged into from nanoparticle in the film network before this, and rate of release is slower.Adopt linking agent cross linking membrane network, make that reactive force is strengthened through becoming key between the membrane component, prevent the too fast release of antiseptic-germicide.
The inventive method is through the antiseptic-germicide heap(ed) capacity; Loading regime; The release profiles of the difference regulation and control antiseptic-germicide of crosslinking degree is taken into account and is reduced the active and long-acting inhibition microorganism growth of Initial microorganisms, and to different foods; Environment and the specific prescription of microbial contamination amount design are with the fungistatic effect optimization.Make up suitable embedding of food proteins nano-antibacterial and delivery vehicles, be not merely the safety-problems that solves biologically active substance a kind of important techniques means are provided, and have important significance for theories for innovative function property development of food.
Summary of the invention
The objective of the invention is to control the speed that antiseptic-germicide discharges from film network through loading multiple means such as dosage, loading regime, crosslinking degree, but the antibacterial film of preparation controlled release.
The present invention can combine two kinds of proteic advantages; Dominant mechanism is: (1) protein film is used to cover food surfaces; And most microorganism growth also reduce influence to food component but add antiseptic-germicide to the protein film useful effect in mikrobe at food surfaces.(2) antiseptic-germicide that directly adds can be discharged into the external world from membrane matrix with fast speed; (3) antiseptic-germicide that adds after the embedding need be discharged into membrane matrix from the nanoparticle of embedding, be discharged into the external world from membrane matrix again; (4) behind the adding linking agent, further Cheng Jian between each component of film, the rate of release of antiseptic-germicide will reduce.Therefore, the present invention directly and indirectly joins antiseptic-germicide in the membrane matrix, prepares dual release, and is effectively antibiotic, and do not influence Food Quality the controlled release antibacterial film of advantageous property.
The present invention adopts following technical scheme to achieve these goals:
A kind of preparation method of controlled release antibacterial film may further comprise the steps:
(1) zein and hydrophobic antimicrobial agent are dissolved in the alcoholic solution;
(2) preparation caseinic acid sodium water solution;
(3) under stirring condition, the caseinic acid sodium water solution is joined in step (1) the gained solution;
(4) with the evaporation of the solution rotating of step (3) gained, centrifugal after, supernatant is carried out lyophilize, obtain nanoparticle powder;
(5) will become membrane matrix water-soluble, add nanoparticle powder and N,O-Diacetylmuramidase, form mixed solution;
(6) add glycerine, pour mask promptly obtains the film product.
The concentration of N,O-Diacetylmuramidase is preferably 0.5-2mg/ml in the said mixed solution of step (5); The preferably glycerine quality is equivalent to the 20%-50% of quality of material summation in the step (5), and said material is for becoming membrane matrix, nanoparticle powder and N,O-Diacetylmuramidase.
Preferably, step (5) also is included in and adds linking agent in the mixed solution, obtains the step of mixed liquid B at 50 ℃-60 ℃ heating 10-240min.
Preferably, the concentration of N,O-Diacetylmuramidase is 0.5-2mg/ml described in the mixed liquid B of step (5) adding linking agent; Qualities of glycerin is equivalent to the 20%-50% of quality of material summation in the step (5), and said material is for becoming membrane matrix, nanoparticle powder, N,O-Diacetylmuramidase and linking agent.
Said linking agent is preferably pycnogenols or TG enzyme.
The said hydrophobic antimicrobial agent of step (1) is preferably thymol or nisin.
Said film forming matrix optimization is sodium-caseinate, gelatin or chitosan.
Heat 0.1-30min at 50 ℃-60 ℃ when preferably, the said one-tenth membrane matrix of step (5) is water-soluble.
The said nanoparticle powder of step (5) is preferably 1:5-1:20 with the mass ratio that becomes membrane matrix.
Preferably, said rotary evaporation temperature is 50-60 ℃, and vacuum tightness is 0.1Mpa, and the time is 10-20min.
The condition that is more preferably is:
Alcoholic solution concentration is preferably 70%-92%v/v;
The mass volume ratio of said zein of step (1) and alcoholic solution is 2.5%～5% g/ml, and the mass volume ratio of hydrophobic antimicrobial agent and alcoholic solution is 0.25%～1% g/ml.The mass volume ratio of sodium-caseinate and water is 0.5%～2% g/ml in the said caseinic acid sodium water solution of step (2);
The mixed volume of the said aqueous solution of step (3) and alcoholic solution is than being 1:1-4:1.
Compare with existing product, the present invention has following beneficial effect:
(1) the present invention is loaded into the nanoparticle of embedding antiseptic-germicide in the membrane matrix, the film mechanicalness is had to a certain degree improve;
(2) the present invention adds antiseptic-germicide in the protein film to, compares directly and adds antiseptic-germicide to food system, has improved the efficient of antiseptic-germicide, has reduced the destruction to food ingredient;
(3) the present invention adopts directly and the mode of indirect dual interpolation, all can effectively control Initial microorganisms and follow-up mikrobe through the dual mode of very fast release and slowly-releasing;
(4) the present invention adopts linking agent to reduce rate of release, has improved the addition upper limit, has prolonged the controlled release time; The regulation and control crosslinking degree can be convenient to further controlled release speed, makes it to be fit to the preservation of food under the special conditions.
Description of drawings
Fig. 1 doesN,O-Diacetylmuramidase and thymol releasing effect in the hybrid films;
Fig. 2 doesN,O-Diacetylmuramidase and thymol releasing effect in the crosslinked hybrid films;
Fig. 3 doesMix the contrast of antibacterial film and antimicrobial nano particle membrane fungistatic effect;
Fig. 4 is AFM figure--5 microns * 5 microns of sweep limits, the scale 200nm of nanoparticle.
Embodiment:
Below in conjunction with embodiment the present invention is described in further detail, but embodiment of the present invention is not limited thereto.
Embodiment 1:
Accurately take by weighing 0.5g zein and 0.1g thymol, be dissolved in 20mL 80% ethanol-water solution; 0.5g sodium-caseinate is dissolved in the 50ml deionized water, it is poured into rapidly in the alcoholic solution in the stirring, in 50 ° of C of temperature, rotary evaporation 20min under the vacuum tightness 0.1Mpa, centrifugal 5min of 8000rpm and freeze-drying.Get the 1g gelatin and be dissolved in the 20ml water, stir 10min down in 60 ° of C, add freeze-drying nanoparticle powder 0.2g, 40mg N,O-Diacetylmuramidase and 0.24g glycerine stir the back pour mask, and at RH40%, 25 ° of C promptly obtained hybrid films down in dry 24 hours.
The physical and chemical performance parameter of the nanoparticle powder that embodiment obtains is following:
Granularity is 182.8nm, and the PDI value is 0.22, and current potential is-39.2mv, and yield is 79.4%, and encapsulation rate is 84.6%.
Nanoparticle powder of the present invention can be used as embedding of food proteins nano-antibacterial and delivery vehicles.
It is as shown in Figure 1 that hybrid films discharges the result.
As can beappreciated from fig. 1, the N,O-Diacetylmuramidase that directly loads discharges very fast, and 12 as a child almost reached 100% release; And the thymol rate of release that loads after the embedding very slowly, and 36 as a child only discharged about 20%.
Embodiment 2:
Accurately take by weighing 0.5g zein and 0.1g thymol, be dissolved in 20mL 70% ethanol-water solution; 0.5g sodium-caseinate is dissolved in the 50ml deionized water, it is poured into rapidly in the alcoholic solution in the stirring, in 60 ° of C of temperature, rotary evaporation 10min under the vacuum tightness 0.1Mpa, centrifugal 10min of 1000rpm and freeze-drying.Get the 1g sodium-caseinate and be dissolved in the 20ml water, add freeze-drying nanoparticle powder 0.2g, the TG enzyme of 40mg N,O-Diacetylmuramidase and 40u stirred 4 hours under 45 ° of C, and 0.48g glycerine stirs the back pour mask again, and at RH40%, 25 ° of C promptly obtained crosslinked hybrid films down in dry 24 hours.
It is as shown in Figure 2 that it discharges the result.
As can beappreciated from fig. 2, the N,O-Diacetylmuramidase that directly loads discharges still very fast, and the thymol rate of release that loads after the embedding is still slower.But speed is compared the release of uncrosslinked film and is reduced greatly.Antibacterial film after crosslinked is fit to preservation term and requires longer food.
The physical and chemical performance parameter of nanoparticle powder is following:
Granularity 182.8 nanometers, PDI is 0.22, current potential for-the 39.2mv yield is 79.4%, encapsulation rate is 84.6%.
Embodiment 3:
Accurately take by weighing the 0.5g zein, be dissolved in 20mL 90% ethanol-water solution; 0.5g sodium-caseinate is dissolved in the 50ml deionized water, it is poured into rapidly in the alcoholic solution in the stirring, in 50 ° of C of temperature, rotary evaporation 20min under the vacuum tightness 0.1Mpa, centrifugal 5min of 8000rpm and freeze-drying.Get freeze-drying nanoparticle powder 0.2g and 1g sodium-caseinate, be dissolved in the 30ml water, add 0.36g glycerine, stir the back pour mask, at RH40%, 25 ° of C promptly obtained sample film down in dry 24 hours.
The physical and chemical performance parameter of nanoparticle powder is following:
Granularity 184.2nm, PDI are 0.22, and current potential is-37.2mv yield 79.6%.
The comparative example 3:
Accurately take by weighing 1.2g sodium-caseinate and 0.36g glycerine, be dissolved in the 30ml water, stir the back pour mask, obtain the sodium-caseinate protein film.
Adopt tensile strength and extensibility to characterize physical strength.Embodiment 1 is as shown in table 1 with the contrast of comparative example's 1 protein film characteristic:
Table 1 loads the antimicrobial nano particle to the mechanical influence of film
| Tensile strength (KPa) | Extensibility (%) | |
| Embodiment 1 mixes antibacterial film | 9.91±1.20 | 33.65±3.99 |
| Comparative example's 1 sodium-caseinate film | 6.43±0.53 | 41.73±5.60 |
Can find out that from table 1 the sodium-caseinate film in the relative comparison example 2 of nano particle membrane tensile strength in the embodiment of the invention 2 has been increased to 9.91KPa from 6.43,, but still keep 33% higher extensibility though extensibility decreases.
Embodiment 4:
Accurately take by weighing 0.5g zein and 0.2g thymol, be dissolved in 20mL 70% ethanol-water solution; 0.5g sodium-caseinate is dissolved in the 50ml deionized water, it is poured into rapidly in the alcoholic solution in the stirring, in 60 ° of C of temperature, rotary evaporation 10min under the vacuum tightness 0.1Mpa, centrifugal 20min of 1000rpm and freeze-drying.Get the 1g gelatin and be dissolved in the 30ml water, 50 ° of C stir 30min, add freeze-drying nanoparticle powder 0.2g, N,O-Diacetylmuramidase 0.1g and 0.48g glycerine, and at RH40%, 25 ° of C promptly obtained hybrid films down in dry 24 hours.
The comparative example 4:
Accurately take by weighing 0.5g zein and 0.2g thymol, be dissolved in 20mL 70% ethanol-water solution; 0.5g sodium-caseinate is dissolved in the 50ml deionized water, it is poured into rapidly in the alcoholic solution in the stirring, in 60 ° of C of temperature, rotary evaporation 10min under the vacuum tightness 0.1Mpa, centrifugal 20min of 1000rpm and freeze-drying.Get lyophilized powder 1.2g, add 0.48g glycerine, be dissolved in the 30ml water, promptly obtain film of nanoparticles
Adopt inhibition zone to represent the size of bacterinertness.Embodiment 4 is as shown in table 2 with the contrast of comparative example's 4 film of nanoparticles characteristics:
Table 2 mixes the contrast of antibacterial film and antimicrobial nano particle membrane inhibition zone size
| Intestinal bacteria inhibition zone (cm) | Salmonellas inhibition zone (cm) | |
| |
27.91±3.11 | 26.55±6.68 |
| Comparative example's 3 film of nanoparticles | 18.81 ± 0.56 | 18.12 ± 1.04 |
Can find out from table 2; For modal pathogenic bacterium in intestinal bacteria and two kinds of food of Salmonellas; Mixing antibacterial film comparative example 1 antimicrobial nano particle membrane in the embodiment of the invention 1 has bigger inhibition zone; This is because because it has better release mode, antiseptic-germicide can be discharged into the extraneous Initial microorganisms that suppresses sooner in the membrane matrix.
Embodiment 5:
Accurately take by weighing 0.5g zein and 0.2g thymol, be dissolved in 20mL 90% ethanol-water solution; 0.5g sodium-caseinate is dissolved in the 50ml deionized water, it is poured into rapidly in the alcoholic solution in the stirring, in 50 ° of C of temperature, rotary evaporation 20min under the vacuum tightness 0.1Mpa, freeze-drying behind the centrifugal 5min of 8000rpm.Get the 1g gelatin and be dissolved in the 20ml water, stir 30min down, add freeze-drying nanoparticle powder 0.2g in 50 ° of C; 0.2g the pycnogenols of N,O-Diacetylmuramidase and 40mg; Be heated to 60 ° of C and stirred 1 hour down, add 0.24g glycerine, stir the back pour mask; At RH40%, 25 ° of C promptly obtained crosslinked hybrid films down in dry 24 hours.
The comparative example 5:
Get the 1g gelatin and be dissolved in the 20ml water, stir 30min down, add the pycnogenols of 0.2g N,O-Diacetylmuramidase and 40mg in 50 ° of C; Be heated to 60 ° of C and stirred 1 hour down, add 0.24g glycerine, stir the back pour mask; At RH40%, 25 ° of C were promptly directly added antibacterial film down in dry 24 hours.
Adopt the staphylococcus aureus culture medium turbidity that adds sample film just to characterize the height of microorganism concn.Having added embodiment 5 hybrid films and comparative example 5, directly to add antibacterial film contrast as shown in Figure 3: as can beappreciated from fig. 3, it is very high that the turbidity of control samples suppresses, kept stable after 1 day; Fungistatic effect is obvious at the beginning for the film carrier that directly adds of comparison example 5, but to losing efficacy after the 3rd day, does not have significant difference with control samples; Embodiment 5 hybrid films kept the good effect that suppresses to streptococcus aureus always in 7 days.
Claims (9)
1. the preparation method of a controlled release antibacterial film is characterized in that, may further comprise the steps:
(1) zein and hydrophobic antimicrobial agent are dissolved in the alcoholic solution;
(2) preparation caseinic acid sodium water solution;
(3) under stirring condition, the caseinic acid sodium water solution is joined in step (1) the gained solution;
(4) with the evaporation of the solution rotating of step (3) gained, centrifugal after, supernatant is carried out lyophilize, obtain nanoparticle powder;
(5) will become membrane matrix water-soluble, add nanoparticle powder and N,O-Diacetylmuramidase, form mixed solution;
(6) add glycerine, pour mask promptly obtains the film product.
2. preparation method according to claim 1 is characterized in that, the concentration of N,O-Diacetylmuramidase is 0.5-2mg/ml in the said mixed solution of step (5); Qualities of glycerin is equivalent to the 20%-50% of quality of material summation in the step (5), and said material is for becoming membrane matrix, nanoparticle powder and N,O-Diacetylmuramidase.
3. preparation method according to claim 1 is characterized in that, step (5) also is included in and adds linking agent in the mixed solution, obtains the step of mixed liquid B at 50 ℃-60 ℃ heating 10-240min.
4. preparation method according to claim 3 is characterized in that, the concentration of N,O-Diacetylmuramidase is 0.5-2mg/ml described in the mixed liquid B of step (5) adding linking agent; Qualities of glycerin is equivalent to the 20%-50% of quality of material summation in the step (5), and said material is for becoming membrane matrix, nanoparticle powder, N,O-Diacetylmuramidase and linking agent.
5. according to the described preparation method of one of claim 1-4, it is characterized in that said linking agent is pycnogenols or TG enzyme.
6. according to the described preparation method of one of claim 1-4, it is characterized in that the said hydrophobic antimicrobial agent of step (1) is thymol or nisin.
7. according to the described preparation method of one of claim 1-4, it is characterized in that said one-tenth membrane matrix is sodium-caseinate, gelatin or chitosan.
8. according to the described preparation method of one of claim 1-4, it is characterized in that, heat 0.1-30min at 50 ℃-60 ℃ when the said one-tenth membrane matrix of step (5) is water-soluble.
9. according to the described preparation method of one of claim 1-4, it is characterized in that the said nanoparticle powder of step (5) is 1:5-1:20 with the mass ratio that becomes membrane matrix.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210248886.2A CN102786702B (en) | 2012-07-18 | 2012-07-18 | Preparation method of controlled release antibacterial film |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210248886.2A CN102786702B (en) | 2012-07-18 | 2012-07-18 | Preparation method of controlled release antibacterial film |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102786702A true CN102786702A (en) | 2012-11-21 |
| CN102786702B CN102786702B (en) | 2014-07-02 |
Family
ID=47152302
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210248886.2A Active CN102786702B (en) | 2012-07-18 | 2012-07-18 | Preparation method of controlled release antibacterial film |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102786702B (en) |
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103421329A (en) * | 2013-08-21 | 2013-12-04 | 华南理工大学 | Preparation method for antimicrobial edible film material |
| CN103431156A (en) * | 2013-08-13 | 2013-12-11 | 华南理工大学 | Zein nanoparticle and preparation method thereof |
| CN104163933A (en) * | 2013-05-17 | 2014-11-26 | 中国科学院海洋研究所 | Polymer film prepared based on freeze drying technology and method and application thereof |
| CN104225669A (en) * | 2014-08-25 | 2014-12-24 | 华南理工大学 | Bioactive bacterial cellulose-zein composite film and preparation method thereof |
| CN104397828A (en) * | 2014-11-19 | 2015-03-11 | 渤海大学 | Preparation method of modified zein composite slow-release antibacterial liquid membrane |
| CN105176102A (en) * | 2015-09-24 | 2015-12-23 | 甘肃农业大学 | Water-soluble casein membrane and preparing technology thereof |
| CN105754118A (en) * | 2016-03-14 | 2016-07-13 | 宁波江东索雷斯电子科技有限公司 | Method for preparing biological antioxidant composite conducting membrane |
| CN108070103A (en) * | 2017-12-18 | 2018-05-25 | 四川农业大学 | A kind of degradable antibiotic property natural complex fresh-keeping film and preparation method thereof |
| CN108587188A (en) * | 2018-04-24 | 2018-09-28 | 山东鲁北药业有限公司 | The preparation method of lysozyme antibiotic film |
| CN109280352A (en) * | 2018-09-17 | 2019-01-29 | 佛山皖和新能源科技有限公司 | A kind of high intensity antimicrobial preservative film and preparation method thereof |
| CN110066520A (en) * | 2019-04-26 | 2019-07-30 | 安徽农业大学 | A kind of anthocyanin nano-complex gelatin edible film delaying Oxidation of Fat and Oils |
| CN110878172A (en) * | 2018-09-05 | 2020-03-13 | 周维 | Edible gelatin sodium caseinate film and preparation method thereof |
| CN113201263A (en) * | 2021-04-12 | 2021-08-03 | 华南理工大学 | Preparation method of super-hydrophobic antibacterial coating and super-hydrophobic antibacterial food manipulator |
| CN113208105A (en) * | 2021-05-10 | 2021-08-06 | 上海交通大学 | Method for improving stability of alcohol soluble protein particles |
| CN113712072A (en) * | 2021-09-02 | 2021-11-30 | 合肥工业大学 | Clove essential oil pickering emulsion coating preservative and preparation method and application thereof |
| CN114158612A (en) * | 2021-12-21 | 2022-03-11 | 广东广益科技实业有限公司 | Areca nut polishing agent, preparation method and application thereof |
| CN116589753A (en) * | 2022-06-09 | 2023-08-15 | 海南师范大学 | Pineapple She Fangshai cellulose membrane and preparation and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070196458A1 (en) * | 2004-06-07 | 2007-08-23 | Jie Zhang | Compositions and methods for dermally treating neuropathic pain |
-
2012
- 2012-07-18 CN CN201210248886.2A patent/CN102786702B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070196458A1 (en) * | 2004-06-07 | 2007-08-23 | Jie Zhang | Compositions and methods for dermally treating neuropathic pain |
Non-Patent Citations (1)
| Title |
|---|
| 赵华等: "玉米醇溶蛋白制备与应用研究进展", 《粮食与饲料工业》 * |
Cited By (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104163933A (en) * | 2013-05-17 | 2014-11-26 | 中国科学院海洋研究所 | Polymer film prepared based on freeze drying technology and method and application thereof |
| CN104163933B (en) * | 2013-05-17 | 2017-10-20 | 中国科学院海洋研究所 | A kind of polymer thin film and its methods and applications prepared based on Freeze Drying Technique |
| CN103431156A (en) * | 2013-08-13 | 2013-12-11 | 华南理工大学 | Zein nanoparticle and preparation method thereof |
| CN103421329A (en) * | 2013-08-21 | 2013-12-04 | 华南理工大学 | Preparation method for antimicrobial edible film material |
| CN103421329B (en) * | 2013-08-21 | 2015-07-01 | 华南理工大学 | Preparation method for antimicrobial edible film material |
| CN104225669A (en) * | 2014-08-25 | 2014-12-24 | 华南理工大学 | Bioactive bacterial cellulose-zein composite film and preparation method thereof |
| CN104225669B (en) * | 2014-08-25 | 2016-04-20 | 华南理工大学 | Biological activity Bacterial cellulose-Compound Film of Zein and preparation method thereof |
| CN104397828A (en) * | 2014-11-19 | 2015-03-11 | 渤海大学 | Preparation method of modified zein composite slow-release antibacterial liquid membrane |
| CN105176102A (en) * | 2015-09-24 | 2015-12-23 | 甘肃农业大学 | Water-soluble casein membrane and preparing technology thereof |
| CN105754118A (en) * | 2016-03-14 | 2016-07-13 | 宁波江东索雷斯电子科技有限公司 | Method for preparing biological antioxidant composite conducting membrane |
| CN105754118B (en) * | 2016-03-14 | 2018-06-05 | 深圳市安伯斯科技有限公司 | A kind of preparation method of biological antioxidant composite conductive film |
| CN108070103A (en) * | 2017-12-18 | 2018-05-25 | 四川农业大学 | A kind of degradable antibiotic property natural complex fresh-keeping film and preparation method thereof |
| CN108587188A (en) * | 2018-04-24 | 2018-09-28 | 山东鲁北药业有限公司 | The preparation method of lysozyme antibiotic film |
| CN110878172A (en) * | 2018-09-05 | 2020-03-13 | 周维 | Edible gelatin sodium caseinate film and preparation method thereof |
| CN109280352A (en) * | 2018-09-17 | 2019-01-29 | 佛山皖和新能源科技有限公司 | A kind of high intensity antimicrobial preservative film and preparation method thereof |
| CN110066520A (en) * | 2019-04-26 | 2019-07-30 | 安徽农业大学 | A kind of anthocyanin nano-complex gelatin edible film delaying Oxidation of Fat and Oils |
| CN110066520B (en) * | 2019-04-26 | 2021-02-26 | 安徽农业大学 | Anthocyanin nano-composite gelatin edible film capable of delaying grease oxidation |
| CN113201263A (en) * | 2021-04-12 | 2021-08-03 | 华南理工大学 | Preparation method of super-hydrophobic antibacterial coating and super-hydrophobic antibacterial food manipulator |
| CN113208105A (en) * | 2021-05-10 | 2021-08-06 | 上海交通大学 | Method for improving stability of alcohol soluble protein particles |
| CN113712072A (en) * | 2021-09-02 | 2021-11-30 | 合肥工业大学 | Clove essential oil pickering emulsion coating preservative and preparation method and application thereof |
| CN113712072B (en) * | 2021-09-02 | 2023-04-25 | 合肥工业大学 | Clove essential oil pickering emulsion film coating preservative and preparation method and application thereof |
| CN114158612A (en) * | 2021-12-21 | 2022-03-11 | 广东广益科技实业有限公司 | Areca nut polishing agent, preparation method and application thereof |
| CN114158612B (en) * | 2021-12-21 | 2024-01-05 | 广东广益科技实业有限公司 | Betel nut polishing agent, and preparation method and application thereof |
| CN116589753A (en) * | 2022-06-09 | 2023-08-15 | 海南师范大学 | Pineapple She Fangshai cellulose membrane and preparation and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102786702B (en) | 2014-07-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102786702B (en) | Preparation method of controlled release antibacterial film | |
| Hu et al. | Formation and optimization of chitosan-nisin microcapsules and its characterization for antibacterial activity | |
| Li et al. | Butylated hydroxyanisole encapsulated in gelatin fiber mats: Volatile release kinetics, functional effectiveness and application to strawberry preservation | |
| Mathew et al. | Photochemical and antimicrobial properties of silver nanoparticle-encapsulated chitosan functionalized with photoactive groups | |
| CN102715154B (en) | Litsea cubeba oil mildew and moth proof slow release microcapsule and preparation method | |
| CN108752610A (en) | A kind of edibility antibacterial film and preparation method thereof of sustained release essential oil | |
| CN113712144A (en) | Preparation method of rose geranium essential oil microcapsule with good oxidation resistance and bacterial inhibition performance | |
| CN107549476A (en) | A kind of high antimicrobial nano feed addictive and preparation method thereof | |
| CN105381500A (en) | Functional wound surface repairing material and preparation method thereof | |
| CN104399067A (en) | Veterinary preparation namely lysozyme-loaded chitosan microspheres and preparation method thereof | |
| CN102604343A (en) | Food antimicrobial packaging material with slow release performance and preparation method of food antimicrobial packaging material | |
| CN103980665A (en) | Waterborne antibacterial composite material and preparation method thereof | |
| CN104758976A (en) | Dual-network hydrogel loaded with thermo-sensitive particle protide medicines and preparation method | |
| Xia et al. | Fabrication and characterization of zein-encapsulated Litsea cubeba oil nanoparticles and its effect on the quality of fresh pork | |
| CN108249420B (en) | A kind of positively charged carbon dots and its preparation method and application | |
| CN112021392A (en) | Composite preservative containing natural essential oil nano chitosan particles and application of composite preservative in sturgeon fillet storage | |
| CN109321622B (en) | Preparation method and application of pseudo-ginseng polypeptide | |
| CN102020779A (en) | Preparation method of edible anti-bacterial packaging film | |
| Babayev et al. | Antimicrobial pseudolatex zein films with encapsulated carvacrol for sustainable food packaging | |
| CN113455504A (en) | Chitosan-loaded caffeic acid ester nano-medicament and preparation method and application thereof | |
| CN113261677A (en) | Antibacterial isolated soy protein-gallic acid nanoparticles and preparation method thereof | |
| CN104784103A (en) | Injectable antibacterial hydrogel based on amphiphilic molecules of oligomeric amino acid | |
| CN109497540A (en) | A method of polyphenol stability is improved using phytoferritin | |
| EP1213347A1 (en) | A method for preserving cells by processing the same into dry products | |
| CN107047972B (en) | Functional feed added with water bloom blue algae extract |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |