CN104964968B - A kind of microbiocidal time temperature indicator for Table Grape quality perception - Google Patents
A kind of microbiocidal time temperature indicator for Table Grape quality perception Download PDFInfo
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- CN104964968B CN104964968B CN201510243990.6A CN201510243990A CN104964968B CN 104964968 B CN104964968 B CN 104964968B CN 201510243990 A CN201510243990 A CN 201510243990A CN 104964968 B CN104964968 B CN 104964968B
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Abstract
The present invention provides a kind of microbiocidal time temperature indicator for Table Grape quality perception, it includes a container, contains uniformly mixed microbial inoculum, growth substrate and pH indicator in container；The microorganism is lactobacillus paracasei；The growth substrate is MRS broth bouillons；The pH indicator is bromocresol green.The activation energy of its activation energy of time temperature indicator provided by the invention and fruits and vegetables is close, and available for the quality comparison monitored in fresh fruits and vegetables Cold Chain Logistics, especially can accurately be used for Table Grape quality perception and monitoring.
Description
Technical field
The present invention relates to Time-temperature-shelf life instruction and quality monitoring technical field, specifically, it is related to a kind of use
In the microbiocidal time temperature indicator of Table Grape quality perception.
Background technology
Time temperature indicator (Time Temperature Indicator, TTI) is that one kind is able to record and tracking life
Time-temperature course during fresh agricultural product logistics, and quilt is directly reflected by the movement of mechanical deformation, color change or color
Monitor the mark and cognition technology of all or part of shelf life of product.Its monitoring result than traditional default shelf-life, most
The marks such as slow selling period are relatively reliable, ensure consumption safety.According to operation principle, TTI can be divided into chemical type, enzyme type, micro- life
Thing type, physical chemistry type, diffused, polymer-type, solid-state reaction type, photaesthesia type TTI.Past research is concentrated mainly on enzyme
Type, chemical type, diffused TTI, and the research on microbiocidal TTI is seldom.
Microbiocidal TTI is generally made of microorganism, pH indicator, growth substrate.It is micro- relative to other kinds of TTI
Biological TTI biggest advantages are that its response process is the process of a simulated foods feature spoilage organisms growth and metabolism.
This shows that microorganism TTI has the standard of higher on the food that monitoring is reduced due to fresh agricultural products quality caused by spoilage organisms
True property.In addition, from the point of view of economical view-point, what large number of microorganism can be relatively inexpensive is made, the cost of manufacture of microorganism TTI
It is low, microorganism TTI can be promoted to be widely used in food quality monitoring.
It is Table Grape thin skin succulence, full of nutrition, microorganism growth, breeding are particularly suitable for, goes mouldy, ash change and produce
Peculiar smell and toxicity, substantially reduce edible value and economic value.China is the big Table Grape main production country of the first in the world, accounts for the world
Vintage 10.8%.The cold chain process of its specification is stringent to temperature requirement：- 2 DEG C~-1 DEG C of precooling requirement, refrigeration then requires 0
DEG C~1 DEG C, transport then requires control below 5 DEG C；It is but also often gentle using gas anti-corrosive fresh-keeping, Calcium treatment preservation technology
Adjust the preservation technologies such as storage.But due to China's Table Grape technology of storage and transport fall behind, by quality reduce or go mouldy wait caused by lose
Up to 25~30%.Therefore, improve Table Grape Cold Chain Logistics, reduce garbage treatment quantity and unhelpful CO caused by rotting2Row
Put, improve environmental protection and ecological benefits；Waste and economic loss are reduced, promotes the development of storage and transportation of grapes material flow industry, is improved
Industry Core Competitive, has important social benefit.
Microorganism TTI can be applied to Table Grape Cold Chain Logistics quality monitoring as a kind of intelligent packaging.Microorganism TTI
Since picking Table Grape, before entirely transport, storage, dispatching, sale are eaten up to consumer, with Table Grape
Identical time-temperature history is undergone, the real-time remaining shelf life of Table Grape is characterized by color change, facilitates sellers to fresh
Timely and effectively decision-making is made in the sale of food grape, reduces waste and economic loss.
Its activation energy range of the microorganism TTI that presently, there are is 82.55~127.28kJ/mol, its activation energy is with eating Portugal raw
The activation energy of the grape difference that compares is larger, it is impossible to the very quality comparison of accurate measurements Table Grape, and indicate its shelf life.
When the activation energy of microorganism TTI and Table Grape are closer, microorganism TTI instructions shelf life effect is more accurate.The application is pair
A kind of improvement of the prior art on Table Grape quality monitoring, is formulated by improving, obtained with Table Grape activation energy more
Close microorganism TTI prototypes.
Lactobacillus paracasei is subordinate to lactobacillus, is a kind of probiotics.The decomposition glucose during growth metabolism, produces
Lactic acid.Microorganism TTI can be made using this property.There is presently no make microorganism TTI using lactobacillus paracasei.
The content of the invention
In order to solve the problems in the existing technology, the object of the present invention is to provide one kind to be used for Table Grape quality sense
The microbiocidal time temperature indicator (TTI) known.
In order to realize the object of the invention, present invention firstly provides during a kind of microbiocidal for Table Grape quality perception
Between temperature indicator, it includes a container, and uniformly mixed microbial inoculum, growth substrate and pH instructions are contained in container
Agent；The microorganism is lactobacillus paracasei；The growth substrate is MRS broth bouillons；The pH indicator is bromine cresols
It is green.
Further, the preparation method of the time temperature indicator includes the following steps：
1) bromocresol green powder is added in growth substrate, obtains being mixed with the MRS solution of pH indicator；
2) microbial inoculum is uniformly mixed with the MRS solution that step 1) obtains, to obtain the final product.
Preferably, the step 1) is：0.039~0.041g bromocresol green powder is added in every liter of growth substrate,
Obtain being mixed with the MRS solution of pH indicator.
Preferably, the step 2) is：The MRS solution that microbial inoculum and step 1) are obtained is using volume ratio as 10-5:
1 ratio is uniformly mixed.
Further, the preparation method of the microbial inoculum is specially：Lactobacillus paracasei dry powder is activated, 37
When DEG C constant temperature incubation 24 is small, bacterium solution is centrifuged, is washed twice with sterile saline, is precipitated, add sterile saline,
Concentration is prepared into as 10-8~10-9The microbial inoculum of CFU/ml；Wherein, culture medium used when actication of culture and culture is
MRS broth bouillons.
Present invention also offers aforesaid time temperature indicator in Table Grape quality perception and the application in monitoring.
Since Table Grape stick the microorganism TTI labels picking, Table Grape is undergone with microorganism TTI
Identical time-temperature history.Entirely transport in Table Grape, store, dispense, sell until consumer eat before during,
The color of microorganism TTI labels constantly changes with the accumulation of time-temperature, and the quality of Table Grape is also with time temperature
The accumulation of degree constantly reduces, when the color of microorganism TTI reaches the color of shelf life terminal, it is meant that Table Grape quality drops
It is low to inedible.
The beneficial effects of the present invention are：
A kind of time temperature is prepared using lactobacillus paracasei, growth substrate and bromocresol green indicator solution in the present invention
Spend indicator.Lactobacillus paracasei is a kind of probiotics, and growth substrate can directly use the MRS meat soups of in the market standardized production
Culture medium, it is convenient and efficient.The microbiocidal time temperature indicator cost of manufacture is cheap, and method is simple, is conducive to extensive life
Production.
The activation energy of its activation energy of time temperature indicator provided by the invention and fruits and vegetables is close, fresh available for monitoring
Quality comparison in fruits and vegetables Cold Chain Logistics, especially can accurately be used for Table Grape quality perception and monitoring.Relative to presently, there are
Microorganism TTI, the microorganism TTI activation energy is with Table Grape activation energy more closely, thereby indicate that effect is more accurate.Phase
For the temperature monitoring technique based on WSN that presently, there are, which has low, small, without power supply power supply the spy of cost
Point.In addition, the microorganism TTI has the characteristics that the quality comparison of monitoring Table Grape in real time and predicts shelf life, convenient sale
Timely and effectively decision-making is made in sale of the person to Table Grape, avoids waste and economic loss.
Brief description of the drawings
Fig. 1 is the pictorial diagram of time temperature indicator of the present invention.
Fig. 2 is color change course of the time temperature indicator of the present invention under 15 DEG C of constant temperatures.
Fig. 3 is that titratable acid concentration (TA) change of the time temperature indicator of the present invention under 15 DEG C of constant temperatures becomes
Gesture figure.
Fig. 4 is total color difference Δ E trend chart of the time temperature indicator of the present invention under 15 DEG C of constant temperatures.
Fig. 5 is pH trend chart of the time temperature indicator of the present invention under 15 DEG C of constant temperatures.
Fig. 6 is pH trend chart of the time temperature indicator of the present invention under 25 DEG C of constant temperatures.
Fig. 7 is pH trend chart of the time temperature indicator of the present invention under 37 DEG C of constant temperatures.
The Arrhenius figures that Fig. 8 is pH in time temperature indicator system of the present invention.
The Arrhenius figures that Fig. 9 is TA in time temperature indicator system of the present invention.
The Arrhenius figures that Figure 10 is Δ E in time temperature indicator system of the present invention.
Figure 11 is the Arrhenius figures of the hardness of rose fragrant Table Grape.
Figure 12 is the Arrhenius figures of the percentage of water loss of rose fragrant Table Grape.
Embodiment
Following embodiments are used to illustrate the present invention, but are not limited to the scope of the present invention.
Embodiment 1
Prepare lactobacillus paracasei bacterium solution：Lactobacillus paracasei dry powder is activated twice, is then cultivated again at 37 DEG C
24 it is small when, bacterium solution is centrifuged, is washed twice with sterile saline, is precipitated, add sterile saline, be prepared into concentration
For 10-8~10-9The microbial inoculum of CFU/ml, it is spare.
Prepare growth substrate：Prepare MRS cultured solution of broth.
Prepare the MRS solution for being mixed with pH indicator：Every liter of MRS broth bouillon adds the bromocresol green powder of 0.04g.
The microbial inoculum is with the MRS solution for being mixed with pH indicator using volume ratio as 10-5:1 is uniformly mixed, packing
Enter container, that is, microorganism TTI is made.
Practicality measures：
The microorganism TTI is placed at 15 DEG C, every 4h or so measure responses (titratable acid concentration, pH, total Se Cha ⊿
E), TTI response parameter trend charts are obtained.
The measure application Hunter Lab systems of TTI colors, L, a, b value are measured using Minolta CR410 colour difference meters, its
Middle L represents brightness, and a represents a axis values in Hunter (Hunter) scale, and b represents the b axis values in Hunter (Hunter) scale ,+a
Represent red ,-a represents green, and+b represents yellow, and-b represents that blueness , represents that (aberration refers to numerical value come table total color difference Yong ⊿ E
Show that two kinds of colors give people the difference on color perception).
E=(⊿ a2+⊿b2+⊿c2)1/2
The measurement of TTI titratable acid concentration：The TTI solution of 5g is diluted with the sterile distilled water of 20ml, with the 1% of 0.5ml
Phenolphthalein it is homogenized (10s), be titrated to pH=8.2 with the NaOH of 0.1mol/L afterwards.
TA (%)=a*b*0.009*100/w
A is the dosage of NaOH, unit ml；B is the molar concentration of NaOH；0.009 is the equivalence factor of lactic acid；W is sample
Product weight (g).
Fig. 2 is the color change course of this indicator TTI systems under 15 DEG C of constant temperatures, figure it is seen that with
The migration of time.This indicator TTI generates the obvious color change from blackish green to yellow.
During Fig. 3 reacts for this indicator TTI systems under 15 DEG C of constant temperatures, titratable acid concentration trend chart, from Fig. 3
As can be seen that increasing over time, titratable acid concentration is in rising trend.
Fig. 4 is under 15 DEG C of constant temperatures in the reaction of this indicator TTI systems, total color difference Δ E trend charts can from Fig. 4
To find out, increase over time, total color difference is in rising trend.
During Fig. 5 reacts for this indicator TTI systems under 15 DEG C of constant temperatures, pH trend charts, from fig. 5, it can be seen that
Increase over time, pH is on a declining curve.
From Fig. 3~5 as can be seen that in the reaction of this indicator TTI systems under 15 DEG C of constant temperatures, titratable acid concentration,
Good linear relationship is presented with time t in total color difference Δ E, pH.
Method according to embodiment prepares microbiocidal time temperature indicator, respectively under the conditions of 15 DEG C, 25 DEG C, 37 DEG C
Carry out constant temperature test.The total color difference of periodic measurement time temperature indicator, pH, titratable acid concentration.
Fig. 5~7 for 15 DEG C in the reaction of this indicator TTI systems, 25 DEG C, under 37 DEG C of constant temperatures, the trend chart of pH,
It can be seen from the figure that the reaction system is at different temperatures, it is in preferable linear relationship that pH, which changes with time, can be obtained
Reaction rate under to different temperatures.
According to Arrhenius equation：- lnk=Ea/RT-lnk0, natural logrithm is taken by reaction rate is obtained in Fig. 3~7,
Again linear fit, opening relationships curve lnk-1/RT, you can when drawing are carried out with absolute temperature and the reciprocal of gas constant product
Between temperature indicator pH, TA, ⊿ E Arrhenius figure, such as Fig. 8~10.Each index activation energy of TTI is directly obtained by straight slope
.PH, the TA, ⊿ E activation energy of TTI is followed successively by 76.052kJ/mol, 79.461kJ/mol, 73.926kJ/mol.
Similarly, the crucial index of quality hardness and percentage of water loss of rose fragrant Table Grape are measured, obtains it
Arrhenius curves, such as Figure 11~12, the work of the hardness, the percentage of water loss index of quality of rose fragrant Table Grape can be obtained by figure
Change can be 66.905kJ/mol, 71.955kJ/mol.The wherein percentage of water loss of Table Grape and microorganism TTI ⊿ E activation energy models
Enclose all in 70~75kJ/mol, and differ 1.971kJ/mol, it is closest.TTI is differed with the activation energy of product in theory
20kJ/mol, TTI can accurately be used for product quality monitoring.Therefore, which can be used for eating raw
Grape quality perceives and monitoring.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (2)
1. a kind of microbiocidal time temperature indicator for Table Grape quality perception, it is characterised in that it includes one
Container, container is interior to contain uniformly mixed microbial inoculum, growth substrate and pH indicator；The microorganism is secondary cheese breast bar
Bacterium；The growth substrate is MRS broth bouillons；The pH indicator is bromocresol green；
The preparation method of the microbiocidal time temperature indicator includes the following steps：
1) 0.039~0.041g bromocresol green powder is added in every liter of growth substrate, the MRS for obtaining being mixed with pH indicator is molten
Liquid；
2) the MRS solution for obtaining microbial inoculum and step 1) is using volume ratio as 10-5:1 ratio is uniformly mixed；
The preparation method of the microbial inoculum is specially：Lactobacillus paracasei dry powder is activated, 37 DEG C of constant temperature incubations 24
Hour, bacterium solution is centrifuged, is washed twice with sterile saline, is precipitated, adds sterile saline, being prepared into concentration is
10-8~10-9The microbial inoculum of CFU/ml；Wherein, culture medium used when actication of culture and culture is MRS broth bouillons.
2. the time temperature indicator described in claim 1 is in Table Grape quality perception and the application in monitoring.
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| CN105651771A (en) * | 2016-01-11 | 2016-06-08 | 中国农业大学 | Agricultural-product quality monitoring method with integration of color sensing and time-temperature indication |
| CN108037116A (en) * | 2017-11-14 | 2018-05-15 | 中国农业大学 | A kind of microorganism type time-temperature indicator card and its preparation method and application |
| CN110777187B (en) * | 2019-10-18 | 2022-10-25 | 江南大学 | Microorganism type time-temperature indicator |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101539553A (en) * | 2009-04-24 | 2009-09-23 | 上海海洋大学 | Method for monitoring shelf life of perishable chill item |
| CN102279061A (en) * | 2010-06-12 | 2011-12-14 | 北京联合大学 | Microorganism type time-temperature indicator card |
| CN102507576A (en) * | 2011-10-31 | 2012-06-20 | 江南大学 | Microbial type time-temperature indicator for low-temperature circulation items |
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| CN100546821C (en) * | 2002-03-07 | 2009-10-07 | 艾利丹尼森公司 | Be used for change color element of time indicating label and production and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101539553A (en) * | 2009-04-24 | 2009-09-23 | 上海海洋大学 | Method for monitoring shelf life of perishable chill item |
| CN102279061A (en) * | 2010-06-12 | 2011-12-14 | 北京联合大学 | Microorganism type time-temperature indicator card |
| CN102507576A (en) * | 2011-10-31 | 2012-06-20 | 江南大学 | Microbial type time-temperature indicator for low-temperature circulation items |
Non-Patent Citations (1)
| Title |
|---|
| 基于化学型时间温度传感器的鲜食葡萄品质监测;傅泽田 等;《中国农业大学学报》;20130628;第18卷(第6期);186-191 * |
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