CN101900685A - Method for manufacturing microbial time-temperature indicator card - Google Patents
Method for manufacturing microbial time-temperature indicator card Download PDFInfo
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- CN101900685A CN101900685A CN2010101985623A CN201010198562A CN101900685A CN 101900685 A CN101900685 A CN 101900685A CN 2010101985623 A CN2010101985623 A CN 2010101985623A CN 201010198562 A CN201010198562 A CN 201010198562A CN 101900685 A CN101900685 A CN 101900685A
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- escherichia coli
- temperature indication
- microbiocidal
- temperature
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Abstract
The invention relates to a method for manufacturing a microbial time-temperature indicator card. A reduction material is generated by metabolism in the process of the growth and reproduction of escherichia coli and can gradually restore and fade methyleneblue serving as an indicator; and the storage time and temperature of food are indicated through the change of colors. The method comprises the following steps of: uniformly mixing escherichia coli liquor, a culture medium and methyleneblue indicating liquor in a certain ratio; injecting a certain amount of mixed liquor into a transparent glass tube or a plastic tube; and sealing the transparent glass tube or the plastic tube to obtain the time-temperature indicator card.
Description
Technical field
The present invention relates to a kind of method for making of microbiocidal time-temperature indication card.Its core is to utilize Escherichia coli metabolism in the growth and breeding process to produce reducing substances, and reducing substances makes progressively reducing decoloration of methylene blue indicator solution, comes instruction time and variation of temperature with the indicating card change in color.This indicating card can be used for monitoring food and the quality of medicine in the cold chain process of circulation.
Background technology
Temperature is determining the shelf-life of food to a great extent, and food often departs from the temperature conditions of recommendation in the production and the process of circulation, though cause food in the shelf-life, food is putrid and deteriorated.Especially for a collection of product, when temperature departed from, it is more serious that potential safety hazard seems.Therefore, in the food production and the process of circulation, for the monitoring of temperature with control extremely important.
The temperature conditions of monitoring food in the whole process of circulation, just can indicate their real safety and quality situation like this, in order to satisfy these requirements, can service time-temperature indication card.
The basic demand of time-temperature indication card is, it can present continuous variation, and its pace of change raises with temperature and increases, and temperature can not reverse when reducing.Simultaneously, it also should have following characteristic;
1, this changes the reaction brought and is convenient to detect;
2, can show in time-the continuous variation of temperature variation;
3, stable, accurate, when being in same temperature, can demonstrate consistent reaction;
4, volume is little, can be used as the part of packaging for foodstuff;
5, cheap;
6, its reaction can Direct observation, also can obtain and use its entrained information apace with instrument;
7, flexible, can select different configurations with different storage times according to different temperature ranges;
8, its reaction is not subjected to the influence of general mechanical collision;
9, this variation can simulate food spoilage degree and remaining shelf life, or is associated with them;
Can be divided into mechanical type, chemical type, enzyme type, microbiocidal, polymer-type, electron chemistry type, diffused etc. by the principle of work indicating card.In the past, the time-temperature indication card of research and development mainly concentrates on three types: diffused, enzyme type and polymer-type, other type especially the microbiocidal time-temperature indication card research seldom.
The time-temperature indicating card can be used for monitoring food in whole process of circulation preservation variation of temperature.When it is attached in the packaging for foodstuff, monitor its variation, just can monitor the variation of food quality.Time-temperature indication card can be used as the evidence whether manufacturer and dealer abide by contract, thereby whether the processing that food experienced of guaranteeing to arrive the retailer place is correct, and simultaneously, it also can be so that the consumer recognizes the quality of food.
Abroad, time-temperature indication card uses for many years.Up to the present, the United States Patent (USP) of time-temperature indication card and international monopoly are existing up to a hundred.In China, the research and development of time-temperature indication card are at the early-stage, and the application of time-temperature indication card still belongs to blank.
The purpose of this paper invention will be developed a kind of microbiocidal time-temperature indication card exactly.
Summary of the invention
Technical characterictic of the present invention is that selected microorganism is Escherichia coli.
Technical characterictic of the present invention is, Escherichia coli metabolism in the growth and breeding process produces reducing substances, and reducing substances can progressively be reduced into the methylene blue indicator solution dark brown.
Technical characterictic of the present invention is, Escherichia coli bacteria liquid evenly mixes with certain proportion with nutrient culture media and methylene blue indicator solution, gets a certain amount of mixed liquor with micro-liquid getting device and packs in transparent glass tube or the plastic tube, and sealing promptly obtains time-temperature indication card.
Technical characterictic of the present invention is that the used Escherichia coli bacteria liquid concentration of Production Time-temperature indication card is 2.27 * 10
7Cfu/ml-2.29 * 10
7In the cfu/ml scope.
Technical characterictic of the present invention is that the used methylene blue indicator solution of Production Time-temperature indication card concentration is 1g/L.
Technical characterictic of the present invention is, the Production Time-the temperature indication card process in, the ratio of Escherichia coli bacteria liquid and nutrient culture media and methylene blue indicator solution is and 3: 10: 1.
Technical characterictic of the present invention is that the amount of the mixed liquor of packing in the time-temperature indication card is 0.5ml.
Technical characterictic of the present invention is, after time-temperature indication card completes, is placed under the 0-4 ℃ of condition and places, and the 1st, 3,5,7,9,11,13,15,17 day change color is as figure, and the color of indicating card no longer changes after the 17th day.
Embodiment
Embodiment 1
The preparation of Escherichia coli nutrient culture media
Take by weighing 0.54g beef extract, 1.8g peptone and 0.9g sodium chloride respectively in the 180ml of drying beaker, and add the distilled water of about 170ml, with nutrient culture media with the electric furnace heated and boiled that has asbestos gauge, and evenly, slowly stirring is fully dissolved it with glass bar.Treat that the nutrient culture media temperature reduces the back and regulates in pH to the 7.0-7.5 scope with the 1mol/LNaOH solution for preparing, add distilled water to 180ml, tie mouth with plastic sheeting with holes and bungee, 20min sterilizes in high-pressure steam sterilizing pan.
Embodiment 2
The preparation of time-temperature indication card
Escherichia coli bacteria liquid and nutrient culture media and the methylene blue indicator solution ratio with 3: 10: 1 is mixed, get the 0.5ml mixed liquor with micro-liquid getting device then and pack in glass tube or the plastic tube, sealing promptly gets time-temperature indication card.
Embodiment 3
The mensuration of time-temperature indication card practicality
Time-temperature indication card placed under the 0-4 ℃ of condition place, observe its change color every day, the indicating card color was blue in the 1st day, along with the prolongation of time, the indicating card color gradually changes, the 17th day, indicating card becomes dark brown, and after the 17th day, it is dark brown constant that indicating card keeps always.This time-temperature indication card can be used for indicating chilled food and the food of medicine shelf-life in 17 days.
Claims (6)
1. the making of a microbiocidal time-temperature indication card is characterized in that: utilize Escherichia coli that the methylene blue indicator solution is progressively faded, indicate food and medicine resting period and temperature by change color.
2. the making of a microbiocidal time-temperature indication card, it is characterized in that Escherichia coli bacteria liquid evenly mixes with certain proportion with nutrient culture media and methylene blue indicator solution, get a certain amount of mixed liquor with micro-liquid getting device then and pack in transparent glass tube or the plastic tube, sealing is made.
3. as the making of claim 1 and 2 described a kind of microbiocidal time-temperature indication cards, it is characterized in that: under 0-4 ℃ of oxygen free condition, Escherichia coli growth and breeding speed is slow, and the reducing substances that metabolism produces slowly increases, and reducing substances progressively is reduced into the methylene blue indicator solution dark brown.
4. as the making of claim 1 and 2 described a kind of microbiocidal time-temperature indication cards, wherein Escherichia coli bacteria liquid concentration is 2.27 * 10
7Cfu/ml-2.29 * 10
7Cfu/ml, methylene blue indicator solution concentration is 1g/L.
5. as the making of claim 1 and 2 described a kind of microbiocidal time-temperature indication cards, the ratio of Escherichia coli bacteria liquid and nutrient culture media and methylene blue indicator solution is 3: 10: 1.
6. as the making of claim 1 and 2 described a kind of microbiocidal time-temperature indication cards, the amount of mixed liquor is 0.5ml in the pipe of wherein packing into.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101985623A CN101900685A (en) | 2010-06-12 | 2010-06-12 | Method for manufacturing microbial time-temperature indicator card |
| CN201110166408A CN102279061A (en) | 2010-06-12 | 2011-06-11 | Microorganism type time-temperature indicator card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101985623A CN101900685A (en) | 2010-06-12 | 2010-06-12 | Method for manufacturing microbial time-temperature indicator card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101900685A true CN101900685A (en) | 2010-12-01 |
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Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101985623A Pending CN101900685A (en) | 2010-06-12 | 2010-06-12 | Method for manufacturing microbial time-temperature indicator card |
| CN201110166408A Pending CN102279061A (en) | 2010-06-12 | 2011-06-11 | Microorganism type time-temperature indicator card |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110166408A Pending CN102279061A (en) | 2010-06-12 | 2011-06-11 | Microorganism type time-temperature indicator card |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102279061A (en) * | 2010-06-12 | 2011-12-14 | 北京联合大学 | Microorganism type time-temperature indicator card |
| CN107703067A (en) * | 2016-08-08 | 2018-02-16 | 波音公司 | Monitor removable aberration observation assembly, the system and method for the incident heat and impact event on composite construction |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2014150045A (en) * | 2012-05-11 | 2016-07-10 | Темптайм Корпорейшн | TWO-FUNCTIONAL THERMO-INDICATOR AND METHOD OF PRODUCTION |
| CN102841086A (en) * | 2012-07-05 | 2012-12-26 | 北京联合大学 | Microbial time-temperature indicator |
| CN104964968B (en) * | 2015-05-14 | 2018-04-13 | 中国农业大学 | A kind of microbiocidal time temperature indicator for Table Grape quality perception |
| CN108037116A (en) * | 2017-11-14 | 2018-05-15 | 中国农业大学 | A kind of microorganism type time-temperature indicator card and its preparation method and application |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005026383A1 (en) * | 2003-09-17 | 2005-03-24 | Cryolog S.A. | Method and device for determining if a product is in condition for use or consumption |
| CN101900685A (en) * | 2010-06-12 | 2010-12-01 | 北京联合大学 | Method for manufacturing microbial time-temperature indicator card |
| CN102087145B (en) * | 2010-11-25 | 2013-05-15 | 北京联合大学 | Method for manufacturing paper type time-temperature indicating card |
-
2010
- 2010-06-12 CN CN2010101985623A patent/CN101900685A/en active Pending
-
2011
- 2011-06-11 CN CN201110166408A patent/CN102279061A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102279061A (en) * | 2010-06-12 | 2011-12-14 | 北京联合大学 | Microorganism type time-temperature indicator card |
| CN107703067A (en) * | 2016-08-08 | 2018-02-16 | 波音公司 | Monitor removable aberration observation assembly, the system and method for the incident heat and impact event on composite construction |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102279061A (en) | 2011-12-14 |
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| PB01 | Publication | ||
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
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Open date: 20101201 |