CN104964953A - Automatic wide-range biological fluorescence analyser - Google Patents
Automatic wide-range biological fluorescence analyser Download PDFInfo
- Publication number
- CN104964953A CN104964953A CN201510164781.2A CN201510164781A CN104964953A CN 104964953 A CN104964953 A CN 104964953A CN 201510164781 A CN201510164781 A CN 201510164781A CN 104964953 A CN104964953 A CN 104964953A
- Authority
- CN
- China
- Prior art keywords
- light source
- imaging system
- automatic
- optical imaging
- control system
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Microscoopes, Condenser (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention relates to an automatic wide-range biological fluorescence analyser, which comprises a CCD image controller, an automatic control system and an opto-mechanical structure; the CCD image controller and the automatic control system are mutually connected and controlled; the automatic control system and the opto-mechanical structure are mutually controlled and connected; the bottom of the opto-mechanical structure is provided with a marble platform, and the marble platform is provided with a machine rack, and a lower part of the machine rack and the marble platform are provided with a light source switching apparatus, a focusing elevating gear as well as an optical imaging system which is controlled and connected by the focusing elevating gear; the light source switching apparatus is provided with a LED light source and a colour filter component, and a side end, at one side of the LED light source, of the machine rack is provided with a fan, and the top of the focusing elevating gear is provided with an objective lens in the optical imaging system; the top of the machine rack is provided with a precision sample bench.
Description
Technical field
The present invention relates to technical field of fluorescence detection, be specifically related to a kind of full-automatic fluorimetric assay for biological materials instrument on a large scale.
Background technology
Fluorescent microscope utilizes a high-luminous-efficiency pointolite, general employing high-pressure sodium lamp or LED are as fluorescence light source, fluorescence light source sends the light of certain wavelength as exciting light after color filter array, fluorescent material in sample is excited, make it the fluorescence launching various different colours, again by the amplification of micro objective and eyepiece, thus realize the observation of sample shape and structure.Strong under backing scape, also easily recognize even if fluorescence is very faint, susceptibility is very high, is mainly used in the researchs such as eucaryotic cell structure, function and chemical composition.Why we can observe sample, are because the fluorescence phenomenon presented after fluorescent material absorbs the luminous energy excited in sample.Therefore, the effect of fluorescent microscope light source is not direct illumination, but as a kind of energy exciting fluorescent material in sample.
But existing fluorescent microscope is owing to being subject to the restriction of objective angular field's size, when using fluorescence microscopy and shooting image, can only take very little specimen area, larger or a fairly large number of biological sample for that size, existing fluorescent microscope cannot meet the demands.Existing fluorescent microscope automaticity is very low simultaneously, all need manual switchover light source and color filter during work, manually find or mobile example observation area, make the work efficiency of instrument very low, cannot carry out sample online observation or the analysis of batch, range of application is limited.Therefore, a kind of full-automatic fluorimetric assay for biological materials instrument on a large scale newly of design is needed badly, to solve prior art above shortcomings.
Summary of the invention
The object of this invention is to provide a kind of full-automatic fluorimetric assay for biological materials instrument on a large scale, to overcome currently available technology above shortcomings.
The object of the invention is to be achieved through the following technical solutions:
A kind of full-automatic fluorimetric assay for biological materials instrument on a large scale, comprise ccd image controller, automatic control system and mechanical-optical setup, described ccd image controller and the mutual control linkage of automatic control system, described automatic control system and the mutual control linkage of mechanical-optical setup, marble platform is provided with bottom wherein said mechanical-optical setup, described marble platform is provided with frame, described frame lower end, marble platform is provided with light source switching mechanism, the optical imaging system of focusing jacking gear and described focusing jacking gear control linkage, described light source switching mechanism is provided with LED light source and color filter elements, the frame side of described LED light source side is provided with fan, described focusing jacking gear top is provided with the object lens in optical imaging system, described frame top is provided with accurate sample stage.
Further, described optical imaging system comprises LED light source, illuminator, fluorescence color filter group, object lens, tube lens, CCD adaptive mirror and CCD target surface.
Further, described optical imaging system is for being inverted imaging system.
Beneficial effect of the present invention is: reasonable in design, have employed high precision sample stage and the color filter switching device shifter of Automatic Control, and make use of ccd image splicing, autoscan imaging and analysis can be carried out to large-scale biological cell sample, greatly improve the work efficiency of fluorescent microscope, achieve it in medical treatment and industrial application simultaneously, extend range of application.
Accompanying drawing explanation
Below in order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment below, apparently, accompanying drawing in the following describes is only some embodiments of the application, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is the structural representation of a kind of full-automatic fluorimetric assay for biological materials instrument on a large scale described in the embodiment of the present invention;
Fig. 2 is the optical imaging system structural representation of a kind of full-automatic fluorimetric assay for biological materials instrument on a large scale described in the embodiment of the present invention.
In figure:
1, ccd image controller; 2, automatic control system; 3, mechanical-optical setup; 4, marble platform; 5, frame; 6, light source switching mechanism; 7, focusing jacking gear; 8, optical imaging system; 9, LED light source; 10, color filter elements; 11, fan; 12, object lens; 13, accurate sample stage; 14, color filter elements; 15, tube lens; 16, CCD adaptive mirror; 17, CCD target surface.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, the every other embodiment that those of ordinary skill in the art obtain, all belongs to the scope of protection of the invention.
The full-automatic fluorimetric assay for biological materials instrument on a large scale of one as shown in Figure 1-2, comprise ccd image controller 1, automatic control system 2 and mechanical-optical setup 3, described ccd image controller 1 and automatic control system 2 control linkage mutually, described automatic control system 2 and mechanical-optical setup 3 control linkage mutually, marble platform 4 is provided with bottom wherein said mechanical-optical setup 3, described marble platform 4 is provided with frame 5, described frame 5 lower end, marble platform 4 is provided with light source switching mechanism 6, the optical imaging system 8 of focusing jacking gear 7 and described focusing jacking gear 7 control linkage, described optical imaging system 8 is for being inverted imaging system, described optical imaging system 8 comprises LED light source 9, illuminator 14, color filter elements 10, object lens 12, tube lens 15, CCD adaptive mirror 16 and CCD target surface 17, described light source switching mechanism 6 is provided with LED light source 9 and color filter elements 10, frame 5 side of described LED light source 9 side is provided with fan 11, described focusing jacking gear 7 top is provided with the object lens 12 in optical imaging system 8, described frame 5 top is provided with accurate sample stage 13.
During concrete use, biological cell sample to be measured is placed on accurate sample stage, and two-dimensional movement is carried out to the sample areas of scanning, start ccd image controller 1, input command, automatic control system 2 sends instruction to accurate sample stage 13, the movement of LED light source 9 and color filter elements 10 controls, and feed back to ccd image controller, namely ccd image controller takes pictures, light source switching mechanism 6 can switch the exciting light of different-waveband, realize multiband light exciting sample, because optical imaging system 8 is inverted imaging system, when sample is placed in object lens 12 upper end, LED light source is by after illuminator, after being filtered by color filter elements 10 again, be radiated on biological specimen, thus excite the fluorescent material in sample, make it the fluorescence launching various different colours, then fluorescence is amplified by object lens 12, and by entering CCD target surface after CCD adaptive mirror, present in the form of images eventually through ccd image controller.
The present invention is not limited to above-mentioned preferred forms; anyone can draw other various forms of products under enlightenment of the present invention; no matter but any change is done in its shape or structure; every have identical with the application or akin technical scheme, all drops within protection scope of the present invention.
Claims (3)
1. a full-automatic fluorimetric assay for biological materials instrument on a large scale, it is characterized in that, comprise ccd image controller (1), automatic control system (2) and mechanical-optical setup (3), described ccd image controller (1) and automatic control system (2) control linkage mutually, described automatic control system (2) and mechanical-optical setup (3) control linkage mutually, wherein said mechanical-optical setup (3) bottom is provided with marble platform (4), described marble platform (4) is provided with frame (5), described frame (5) lower end, marble platform (4) is provided with light source switching mechanism (6), the optical imaging system (8) of focusing jacking gear (7) and described focusing jacking gear (7) control linkage, described light source switching mechanism (6) is provided with LED light source (9) and color filter elements (10), frame (5) side of described LED light source (9) side is provided with fan (11), described focusing jacking gear (7) top is provided with the object lens (12) in optical imaging system (8), described frame (5) top is provided with accurate sample stage (13).
2. full-automatic fluorimetric assay for biological materials instrument on a large scale according to claim 1, it is characterized in that, described optical imaging system (8) comprises LED light source (9), illuminator (14), color filter elements (10), object lens (12), tube lens (15), CCD adaptive mirror (16) and CCD target surface (17).
3. full-automatic fluorimetric assay for biological materials instrument on a large scale according to claim 2, is characterized in that, described optical imaging system (8) is for being inverted imaging system.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510164781.2A CN104964953A (en) | 2015-04-09 | 2015-04-09 | Automatic wide-range biological fluorescence analyser |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510164781.2A CN104964953A (en) | 2015-04-09 | 2015-04-09 | Automatic wide-range biological fluorescence analyser |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104964953A true CN104964953A (en) | 2015-10-07 |
Family
ID=54219000
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510164781.2A Pending CN104964953A (en) | 2015-04-09 | 2015-04-09 | Automatic wide-range biological fluorescence analyser |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104964953A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107102157A (en) * | 2017-05-09 | 2017-08-29 | 深圳博大博聚科技有限公司 | A kind of full-automatic fluorescence microscopic analysis instrument |
| CN110146478A (en) * | 2019-06-17 | 2019-08-20 | 王金申 | A kind of on-Line Monitor Device of biological culture dish |
| CN114066818A (en) * | 2021-10-23 | 2022-02-18 | 广州市艾贝泰生物科技有限公司 | Cell detection analysis method, cell detection analysis device, computer equipment and storage medium |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004070366A1 (en) * | 2003-02-04 | 2004-08-19 | Inabata & Co., Ltd. | Fluorescence imaging system and biomanupilator system using same |
| CN1815186A (en) * | 2006-03-08 | 2006-08-09 | 东华大学 | Multifunction chip-detecting apparatus |
| CN101363839A (en) * | 2008-09-12 | 2009-02-11 | 首都师范大学 | Non-damage micrometering system for observating biological sample with fluorescent |
| CN204705589U (en) * | 2015-04-09 | 2015-10-14 | 苏州飞时曼精密仪器有限公司 | A kind of full-automatic fluorimetric assay for biological materials instrument on a large scale |
-
2015
- 2015-04-09 CN CN201510164781.2A patent/CN104964953A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004070366A1 (en) * | 2003-02-04 | 2004-08-19 | Inabata & Co., Ltd. | Fluorescence imaging system and biomanupilator system using same |
| CN1815186A (en) * | 2006-03-08 | 2006-08-09 | 东华大学 | Multifunction chip-detecting apparatus |
| CN101363839A (en) * | 2008-09-12 | 2009-02-11 | 首都师范大学 | Non-damage micrometering system for observating biological sample with fluorescent |
| CN204705589U (en) * | 2015-04-09 | 2015-10-14 | 苏州飞时曼精密仪器有限公司 | A kind of full-automatic fluorimetric assay for biological materials instrument on a large scale |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107102157A (en) * | 2017-05-09 | 2017-08-29 | 深圳博大博聚科技有限公司 | A kind of full-automatic fluorescence microscopic analysis instrument |
| CN110146478A (en) * | 2019-06-17 | 2019-08-20 | 王金申 | A kind of on-Line Monitor Device of biological culture dish |
| CN110146478B (en) * | 2019-06-17 | 2020-03-24 | 滨州医学院 | Online monitoring device of microorganism culture dish |
| CN114066818A (en) * | 2021-10-23 | 2022-02-18 | 广州市艾贝泰生物科技有限公司 | Cell detection analysis method, cell detection analysis device, computer equipment and storage medium |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2256534B1 (en) | In-vivo examination apparatus | |
| US7630065B2 (en) | Low-light specimen image pickup unit and low-light specimen image pickup apparatus | |
| JP5247950B1 (en) | Beam splitter and observation device | |
| EP2796917A1 (en) | A method for automated platform and/or reference object independent acquisition of positional information and localization of objects of interest in a microscope | |
| EP1686380B1 (en) | Robotic apparatus for picking of cells with integrated spectroscopic capability and use thereof | |
| US20150185456A1 (en) | Microscope system and control method therefor | |
| CN104964953A (en) | Automatic wide-range biological fluorescence analyser | |
| CN208044181U (en) | A kind of automatically scanning inverted fluorescence microscope mechanical-optical setup and light channel structure | |
| CN206074895U (en) | Micro- amplification system | |
| JP2012128295A (en) | Optical measuring device | |
| JP2010091809A (en) | Microscope apparatus | |
| CN204705589U (en) | A kind of full-automatic fluorimetric assay for biological materials instrument on a large scale | |
| JP6952891B2 (en) | Carousel for 2x3 and 1x3 slides | |
| US11314072B2 (en) | Microscope system with light sheet microscopy functional unit | |
| EP2594982B1 (en) | Microscope system | |
| CN203054338U (en) | Slide glass with grid reference lines | |
| Churchman et al. | Single-molecule high-resolution colocalization of single probes | |
| TW201732366A (en) | Imaging arrangement determination method for imaging device and imaging device | |
| WO2020126637A3 (en) | Light sheet microscope with turreted lenses | |
| CN110161671B (en) | Dark field, bright field, phase contrast and fluorescence multimode synchronous imaging microscopic imaging device | |
| WO2017170805A1 (en) | Multi-surface image acquisition system, observation device, observation method, screening method, and stereoscopic reconstruction method of subject | |
| CN210376144U (en) | Dual-waveband fluorescence automatic detector | |
| CN116802463A (en) | Universal multi-functional detection system for microwell plates with confocal imaging | |
| JP2018105936A (en) | Microscope and method for supporting setting | |
| KR20220038577A (en) | Physical Calibration Slides |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151007 |