CN104946726A - Method for measuring rice wine rice-koji diastatic power - Google Patents

Abstract

The invention relates to a method for measuring rice wine rice-koji diastatic power. The method comprises the following steps: (1) preparing rice-koji leachate; (2) measuring diastatic power: absorbing starch solution in a volumetric flask, performing preheating, adding the rice-koji leachate, shaking up the mixture and performing timing, performing saccharification for 1 h and then adding NaOH solution to stop enzyme reaction, performing cooling and then performing constant volume to the scale; (3) performing glucose measurement; and (4) enabling a calculation formula to be the milligram amount of soluble starch hydrolysis into glucose in an hour by 1 g of absolute dry rice-koji at 30 DEG C at a pH of 4.6, wherein diastatic power=(V0-V1)*C*(100/5)*(1/w)*1000. The method can accurately and effectively measure the rice wine rice-koji diastatic power and lay the basis for brewing excellent rice wine.

Description

A kind of measuring method of yellow distiller's yeast saccharogenic power

Technical field

The present invention relates to a kind of measuring method of yellow distiller's yeast saccharogenic power, belong to the technical field of brewing yellow rice wine.

Background technology

Meter Qu is not only the saccharifying agent in brewing yellow rice wine, small portion starter, also gives the local flavor of yellow rice wine uniqueness simultaneously.Because meter Qu occupies extremely important status in brewing yellow rice wine, the quality of its quality, directly has influence on the Quality and yield of yellow rice wine, is therefore likened to visually " bone of wine ".Therefore, the saccharogenic power measuring meter Qu is very necessary.

Summary of the invention

For the above-mentioned technical problem of prior art, the object of this invention is to provide a kind of measuring method of yellow distiller's yeast saccharogenic power, the saccharogenic power of the yellow distiller's yeast of Accurate Determining, for the yellow rice wine brewageing high-quality is laid a good foundation.

For achieving the above object, the present invention is achieved by the following technical solutions:

A measuring method for yellow distiller's yeast saccharogenic power, comprises the following steps:

The preparation of (1) 5% meter of bent leach liquor: get the bent 5.815g of rice containing the bent 5g of over dry rice, add in 250mL beaker, the Acetic acid-sodium acetate damping fluid 10mL of 90mL and pH4.6 that add water, stir, heat-insulation soaking l hour in 30 DEG C of water-baths, filter paper filtering, filtrate 4 DEG C saves backup;

The compound method of described Acetic acid-sodium acetate damping fluid is: measure 30mL acetic acid, and thin up is also settled to 1000mL, separately takes 34g sodium-acetate, is dissolved in water and is settled to 500mL, by for subsequent use for two solution mixings;

(2) saccharification:

Get the volumetric flask of 4 200 mL, numbering, be wherein used as sample determination for 1, No. 2, do blank determination for 3, No. 4, every bottle adds 2% Zulkovsky starch solution 100mL;

In 1, No. 2 bottle, add Acetic acid-sodium acetate damping fluid 10mL, shake up, after being incubated 20min, adding the bent leach liquor of 5mL rice, shake up, be incubated saccharification 30min in 20 DEG C of water-baths in 20 DEG C of water-baths, soaking time is counted from adding the bent leach liquor of rice; Saccharification terminates, and adds 4mL 1mol/L NaOH solution immediately, to stop the activity of enzyme, shakes up, be settled to 200mL with water;

To in 3, No. 4 bottles, add 0.65mL 1mol/L NaOH solution, shake up, respectively add the bent leach liquor of 5mL rice, shake up, be settled to 200mL with water;

(3) mensuration of saccharogenic power:

A, blank: draw 25mL2% starch solution in 50mL volumetric flask, then add 15mL0.1mol/LNa0H solution and the bent leach liquor of 5mL5% rice, be settled to scale;

B, sample: draw 25mL2% starch solution in 50mL volumetric flask, 30 DEG C of preheatings 10 minutes, add the bent leach liquor of 5mL5% rice, shake up timing, and 30 DEG C add 15mL0.1mol/LNa0H solution after saccharification l hour and stop enzyme reaction, are settled to scale after cooling;

(4) fixed sugar:

A, blank solution: draw each 5mL of fehling reagent first and second liquid and put into 150mL triangular flask, accurately add 1mL blank to shake up, electric furnace is heated to boiling, be titrated to blue disappearance with 0.1% Glucose standards solution immediately, this operates in 1 minute and completes, and the Glucose standards liquor capacity of wastage in bulk or weight is V ₀mL;

B, saccharified liquid: accurately draw 1.0mL saccharified liquid and replace above-mentioned 1.0mL blank, all the other operations are identical with a, and wastage in bulk or weight Glucose standards liquor capacity is V ₁mL;

(5) calculation formula: the bent saccharogenic power of rice: 1g over dry rice is bent, and pH4.6, is hydrolyzed the milligram number that Zulkovsky starch is glucose in 1 hour at 30 DEG C,

Saccharogenic power=(V ₀-V ₁) × C × (50/1) × (100/5) × (1/w) × l000

V ₀: the Glucose standards liquor capacity of blank wastage in bulk or weight, unit is mL

V ₁: the Glucose standards liquor capacity of sample wastage in bulk or weight, unit is mL

C: Standard glucose solution concentration, unit is g/mL

W: the quality of over dry rice song, unit is g.

Beneficial effect of the present invention is as follows:

The measuring method of the yellow distiller's yeast saccharogenic power of the present invention, can measure the saccharogenic power of yellow distiller's yeast, accurately and efficiently for the yellow rice wine brewageing high-quality is laid a good foundation.

Embodiment

Below in conjunction with specific embodiment, the present invention is further illustrated, but protection scope of the present invention is not limited to this.

The measuring method of the yellow distiller's yeast saccharogenic power of the present invention, comprises the following steps:

The preparation of (1) 5% meter of bent leach liquor: get the bent 5.815g of rice containing the bent 5g of over dry rice, add in 250mL beaker, the Acetic acid-sodium acetate damping fluid 10mL of 90mL and pH4.6 that add water, stir, heat-insulation soaking l hour in 30 DEG C of water-baths, filter paper filtering, filtrate 4 DEG C saves backup;

The compound method of described Acetic acid-sodium acetate damping fluid is: measure 30mL acetic acid, and thin up is also settled to 1000mL, separately takes 34g sodium-acetate, is dissolved in water and is settled to 500mL, by for subsequent use for two solution mixings;

(2) saccharification:

Get the volumetric flask of 4 200 mL, numbering, be wherein used as sample determination for 1, No. 2, do blank determination for 3, No. 4, every bottle adds 2% Zulkovsky starch solution 100mL;

In 1, No. 2 bottle, add Acetic acid-sodium acetate damping fluid 10mL, shake up, after being incubated 20min, adding the bent leach liquor of 5mL rice, shake up, be incubated saccharification 30min in 20 DEG C of water-baths in 20 DEG C of water-baths, soaking time is counted from adding the bent leach liquor of rice; Saccharification terminates, and adds 4mL 1mol/L NaOH solution immediately, to stop the activity of enzyme, shakes up, be settled to 200mL with water;

To in 3, No. 4 bottles, add 0.65mL 1mol/L NaOH solution, shake up, respectively add the bent leach liquor of 5mL rice, shake up, be settled to 200mL with water;

(3) mensuration of saccharogenic power:

A, blank: draw 25mL2% starch solution in 50mL volumetric flask, then add 15mL0.1mol/LNa0H solution and the bent leach liquor of 5mL5% rice, be settled to scale;

B, sample: draw 25mL2% starch solution in 50mL volumetric flask, 30 DEG C of preheatings 10 minutes, add the bent leach liquor of 5mL5% rice, shake up timing, and 30 DEG C add 15mL0.1mol/LNa0H solution after saccharification l hour and stop enzyme reaction, are settled to scale after cooling;

(4) fixed sugar:

The mensuration of a, blank solution: draw each 5mL of fehling reagent first and second liquid and put into 150mL triangular flask, accurately add 1mL blank to shake up, electric furnace is heated to boiling, blue disappearance is titrated to immediately with 0.1% Glucose standards solution, this operates in 1 minute and completes, and the Glucose standards liquor capacity of wastage in bulk or weight is V ₀mL;

The mensuration of b, saccharified liquid: accurately draw 1.0mL saccharified liquid and replace above-mentioned 1.0mL blank, all the other operations are identical with a, and wastage in bulk or weight Glucose standards liquor capacity is V ₁mL;

(5) calculation formula: the bent saccharogenic power of rice: 1g over dry rice is bent, and pH4.6, is hydrolyzed the milligram number that Zulkovsky starch is glucose in 1 hour at 30 DEG C,

Saccharogenic power=(V ₀-V ₁) × C × (50/1) × (100/5) × (1/w) × l000

V ₀: the Glucose standards liquor capacity of blank wastage in bulk or weight, unit is mL

V ₁: the Glucose standards liquor capacity of sample wastage in bulk or weight, unit is mL

C: Standard glucose solution concentration, unit is g/mL

W: the quality of over dry rice song, unit is g.

The measuring method of the yellow distiller's yeast saccharogenic power of the present invention, can measure the saccharogenic power of yellow distiller's yeast, accurately and efficiently for the yellow rice wine brewageing high-quality is laid a good foundation.

Above-described embodiment only illustrates inventive concept of the present invention for explaining, but not the restriction to rights protection of the present invention, all changes utilizing this design the present invention to be carried out to unsubstantiality, all should fall into protection scope of the present invention.

Claims (1)

1. a measuring method for yellow distiller's yeast saccharogenic power, is characterized in that comprising the following steps:

The preparation of (1) 5% meter of bent leach liquor: get the bent 5.815g of rice containing the bent 5g of over dry rice, add in 250mL beaker, the Acetic acid-sodium acetate damping fluid 10mL of 90mL and pH4.6 that add water, stir, heat-insulation soaking l hour in 30 DEG C of water-baths, filter paper filtering, filtrate 4 DEG C saves backup;

The compound method of described Acetic acid-sodium acetate damping fluid is: measure 30mL acetic acid, and thin up is also settled to 1000mL, separately takes 34g sodium-acetate, is dissolved in water and is settled to 500mL, by for subsequent use for two solution mixings;

(2) saccharification:

Get the volumetric flask of 4 200 mL, numbering, be wherein used as sample determination for 1, No. 2, do blank determination for 3, No. 4, every bottle adds 2% Zulkovsky starch solution 100mL;

In 1, No. 2 bottle, add Acetic acid-sodium acetate damping fluid 10mL, shake up, after being incubated 20min, adding the bent leach liquor of 5mL rice, shake up, be incubated saccharification 30min in 20 DEG C of water-baths in 20 DEG C of water-baths, soaking time is counted from adding the bent leach liquor of rice; Saccharification terminates, and adds 4mL 1mol/L NaOH solution immediately, to stop the activity of enzyme, shakes up, be settled to 200mL with water;

To in 3, No. 4 bottles, add 0.65mL 1mol/L NaOH solution, shake up, respectively add the bent leach liquor of 5mL rice, shake up, be settled to 200mL with water;

(3) mensuration of saccharogenic power:

A, blank: draw 25mL2% starch solution in 50mL volumetric flask, then add 15mL0.1mol/L Na0H solution and the bent leach liquor of 5mL5% rice, be settled to scale;

B, sample: draw 25mL2% starch solution in 50mL volumetric flask, 30 DEG C of preheatings 10 minutes, add the bent leach liquor of 5mL5% rice, shake up timing, and 30 DEG C add 15mL0.1mol/L Na0H solution after saccharification l hour and stop enzyme reaction, are settled to scale after cooling;

(4) fixed sugar:

A, blank solution: draw each 5mL of fehling reagent first and second liquid and put into 150mL triangular flask, accurately add 1mL blank to shake up, electric furnace is heated to boiling, be titrated to blue disappearance with 0.1% Glucose standards solution immediately, this operates in 1 minute and completes, and the Glucose standards liquor capacity of wastage in bulk or weight is V ₀mL;

B, saccharified liquid: accurately draw 1.0mL saccharified liquid and replace above-mentioned 1.0mL blank, all the other operations are identical with a, and wastage in bulk or weight Glucose standards liquor capacity is V ₁mL;

(5) calculation formula: the bent saccharogenic power of rice: 1g over dry rice is bent, and pH4.6, is hydrolyzed the milligram number that Zulkovsky starch is glucose in 1 hour at 30 DEG C,

Saccharogenic power=(V ₀-V ₁) × C × (50/1) × (100/5) × (1/w) × l000

V ₀: the Glucose standards liquor capacity of blank wastage in bulk or weight, unit is mL

V ₁: the Glucose standards liquor capacity of sample wastage in bulk or weight, unit is mL

C: Standard glucose solution concentration, unit is g/mL

W: the quality of over dry rice song, unit is g.