CN102608258B - Method for measuring starch content in fermented grains - Google Patents
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Abstract
The invention relates to a method for measuring the starch content in fermented grains, which belongs to the technical field of brewing and aims at solving the technical problem that a measurement result of the starch content in the fermented grains is rough when the starch content in the fermented grains is measured by the existing hydrochloric acid hydrolysis method. The method comprises the steps of a, treating a sample; b, carrying out enzymolysis; c, carrying out hydrochloric acid digestion; and d, measuring reducing sugar. According to the method, the more precise method for measuring the starch content in fermented grains is provided for the brewing field, so that the accuracy of the measurement result can be ensured, and important guide roles on the actual production management can be realized.
Description
Technical field
The invention belongs to brewing technical field, be specifically related to the assay method of poor unstrained spirits content of starch.
Background technology
Aromatic Chinese spirit adopts the process of continuous grain batching, and poor unstrained spirits of making wine becomes to be grouped into primarily of two kinds, grain and chaff shell, and often wheel constantly adds fresh grain and chaff shell in producing in poor unstrained spirits.When wherein entering to store, grain unstrained spirits Middle nutrition component content is higher, and after a round fermentation, nutrition is utilized by Institute of Micro-biology gradually, and when going out to store, grain unstrained spirits Middle nutrition component content is very low, and especially after long-term fermentation more than half a year, poor unstrained spirits Middle nutrition composition almost exhausts.The change of content of starch in grain unstrained spirits, can reflect nutritional labeling composition and the fermentation appearance of poor unstrained spirits well, be Testing index important in wine brewing production run, have good directive function for production management.
Usually, the mensuration of content of starch in poor unstrained spirits of making wine mainly adopts Hydrochloric Acid Hydrolysis Method.The method directly utilizes hydrochloric acid to the fresh poor unstrained spirits effect of being hydrolyzed, starch digestion is become reducing sugar, the reducing sugar (glucose, fructose and maltose) of fehling reagent and solubility is under the condition heated, bolarious copper oxidule precipitation can be generated, therefore measure content of reducing sugar by Fehling Regent method, and then convert out the content of starch.Hydrochloric acid digests in poor unstrained spirits while starch granules, has also carried out digestion to a large amount of hemicellulose in poor unstrained spirits chaff shell or a small amount of cellulose, has caused surveyed content of starch often high than actual conditions.Such as, detected by the method and lose in grain content of starch still about 5 ~ 8% after long-term fermentation, and in real process, starch almost exhausts, this seriously departs from practical condition.Therefore, although the method is easy and simple to handle, testing result is comparatively coarse, higher compared with actual conditions, be difficult to reflect the content of starch in poor unstrained spirits exactly, nutritional labeling composition and the fermentable situation of poor unstrained spirits can not be reflected well, affecting the management to producing and guidance.
Therefore, the non-starch such as digest cellulose, hemicellulose composition while of how avoiding in hydrochloric acid digestion process is the key improving Hydrochloric Acid Hydrolysis Method accuracy.
Summary of the invention
Technical matters to be solved by this invention be existing Hydrochloric Acid Hydrolysis Method when measuring content of starch in poor unstrained spirits testing result coarse, be difficult to indicate the content of starch in poor unstrained spirits exactly, causing nutritional labeling composition and the fermentation appearance that can not reflect poor unstrained spirits well, affecting the management guidance to producing.
The technical scheme of technical solution problem of the present invention is to provide a kind of assay method of poor unstrained spirits content of starch, and the method comprises the following steps:
The process of a, sample: dry poor unstrained spirits, removes fat and soluble sugar, gelatinization;
B, enzymolysis: by adding diastase reaction in the sample after step a gelatinization, filter, collect filtrate, filtrate volume is V1;
C, hydrochloric acid digest: from the filtrate that step b obtains, get the filtrate that volume is V2, add hydrochloric acid, fully digest, be neutralized to neutrality with sodium hydroxide solution, obtain digestive juice, its volume is V3;
The assay method of d, reducing sugar: get the digestive juice that volume is V4 from the digestive juice obtained of step c, adopts fehling reagent titrimetry, detects content of reducing sugar, and calculate content of starch, computing formula is:
a1 is the quality of reducing sugar in sample; A2 is the quality of reducing sugar in blank reagent; 0.9 when being with glucose meter reducing sugar, is converted into the reduction coefficient of starch; S is content of starch, represents with %; The quality of the drying grain unstrained spirits that m takes when being and removing fat and soluble sugar, x is the water cut of dry front poor unstrained spirits.
Wherein, the drying grain unstrained spirits described in step a refers to: fresh poor unstrained spirits to be measured is placed in baking oven, dries to constant weight.
Wherein, the removal fat described in step a and soluble sugar refer to: take dry rear poor unstrained spirits, ether lixiviate, then use 100mL85% ethanol purge.
Preferably, ether lixiviate adopts apparatus,Soxhlet's, more than lixiviate 6h.
Wherein, the gelatinization described in step a refers to: be dissolved in distilled water by the poor unstrained spirits removing fat and soluble sugar, the consumption of distilled water is more than 10 times of dry poor unstrained spirits weight, is heated to 90 ~ 100 DEG C, keeps 30min.
Wherein, the diastase described in step b is at least one in AMS, beta amylase, carbohydrase or amylopectase.
Preferably, the diastase described in step b is AMS and amylopectase.
Preferably, AMS is Thermostable α-Amylase.
Concrete, diastase reaction described in step b is Thermostable α-Amylase reaction, its reaction conditions is as follows: regulate pH to be 6.0 ~ 7.0 with the phosphate buffer that pH is 7.0, the dry poor unstrained spirits of every 1g uses the Thermostable α-Amylase of more than 2000 Mei Huo units, temperature control 90 ~ 100 DEG C, reaction 1 ~ 2h.
Concrete, diastase reaction described in step b is for amylopectin enzyme reaction, and its reaction conditions is as follows: be that 4.5 acetic acid-sodium acetate buffer solution regulates pH to be 4.5 ~ 5.5 with pH, and the poor unstrained spirits of drying of every 1g uses the amylopectase of more than 1000 Mei Huo units, temperature control 55 ~ 60 DEG C, reaction 2h.
The invention has the beneficial effects as follows: adopt the inventive method to detect content of starch in poor unstrained spirits, testing result is accurate, the content of starch in poor unstrained spirits can be indicated exactly, nutritional labeling composition and the fermentation appearance of poor unstrained spirits are reflected well, for wine brewing field provides the method for more accurate, feasible detection grain unstrained spirits content of starch, significant to the management guidance produced.
Embodiment
The assay method of poor unstrained spirits content of starch in the present invention, the method comprises the following steps:
The process of a, sample: dry poor unstrained spirits, removes fat and soluble sugar, gelatinization;
B, enzymolysis: by adding diastase reaction in the sample after step a gelatinization, filter, collect filtrate, filtrate volume is V1;
C, hydrochloric acid digest: from the filtrate that step b obtains, get the filtrate that volume is V2, add hydrochloric acid, fully digest, be neutralized to neutrality with sodium hydroxide solution, obtain digestive juice, its volume is V3;
The assay method of d, reducing sugar: get the digestive juice that volume is V4 from the digestive juice obtained of step c, adopts fehling reagent titrimetry, detects content of reducing sugar, and calculate content of starch, computing formula is:
a1 is the quality of reducing sugar in sample; A2 is the quality of reducing sugar in blank reagent; 0.9 when being with glucose meter reducing sugar, is converted into the reduction coefficient of starch; S is content of starch, represents with %; The quality of the drying grain unstrained spirits that m takes when being and removing fat and soluble sugar, x is the water cut of dry front poor unstrained spirits.
Wherein, the drying grain unstrained spirits described in step a refers to: fresh poor unstrained spirits to be measured is placed in baking oven, dries to constant weight.
Wherein, the removal fat described in step a and soluble sugar refer to: take dry rear poor unstrained spirits, ether lixiviate, then use 100mL85% ethanol purge.
Preferably, ether lixiviate adopts apparatus,Soxhlet's, more than lixiviate 6h.
Wherein, the gelatinization described in step a refers to: be dissolved in distilled water by the poor unstrained spirits removing fat and soluble sugar, the consumption of distilled water is more than 10 times of dry poor unstrained spirits weight, is heated to 90 ~ 100 DEG C, keeps 30min, makes the complete gelatinization of starch wherein.
Wherein, the diastase described in step b is at least one in AMS, beta amylase, carbohydrase or amylopectase.
Preferably, the diastase described in step b is AMS and amylopectase.
Preferably, AMS is Thermostable α-Amylase.
Concrete, diastase reaction described in step b is Thermostable α-Amylase reaction, its reaction conditions is as follows: regulate pH to be 6.0 ~ 7.0 with the phosphate buffer that pH is 7.0, the dry poor unstrained spirits of every 1g uses the Thermostable α-Amylase of more than 2000 Mei Huo units, temperature control 90 ~ 100 DEG C, reaction 1 ~ 2h.
Concrete, diastase reaction described in step b is for amylopectin enzyme reaction, and its reaction conditions is as follows: be that 4.5 acetic acid-sodium acetate buffer solution regulates pH to be 4.5 ~ 5.5 with pH, and the poor unstrained spirits of every 1g drying uses the amylopectase of more than 1000 Mei Huo units, temperature control 55 ~ 60 DEG C, reaction 2h.
The present invention is before hydrochloric acid digestion, adopt the mode of enzymolysis single-minded make Starch Hydrolysis, obtain the residual chain of starch or reducing sugar, again through filtering, eliminate other impurity except starch in sample before hydrochloric acid hydrolysis, thus when avoiding only employing hydrochloric acid digestion or first adopt hydrochloric acid digestion, by the decomposition of other materials such as the hemicellulose in poor unstrained spirits or cellulose, have impact on testing result.Secondly, in conjunction with hydrochloric acid digestion after enzymolysis, ensure that the residual chain of starch obtains complete hydrolysis, guarantee the accuracy of testing result.
In the present invention, dry again after claiming fresh weight by fresh poor unstrained spirits to be measured, dry the quality weighing dry poor unstrained spirits to constant weight and obtain dry weight, can be used for the water cut calculating poor unstrained spirits.
In the present invention, after dry, poor unstrained spirits is by ether lixiviate and ethanol purge, can remove the fat in poor unstrained spirits and soluble sugar, reduce the interference to testing result.When adopting ether lixiviate, in order to save solvent use amount, improving extracting efficiency, can apparatus,Soxhlet's be adopted, determine the consumption of ether according to the specification of apparatus,Soxhlet's.
In the present invention, starch is water insoluble at normal temperatures, but at high temperature swelling, division forms the characteristic of homogeneous paste solution, is called the gelatinization of starch.If not by gelatinization, then starch granules is insoluble in water, is unfavorable for the effect of diastase to starch molecule.
In the present invention, diastase mainly comprises AMS (α-Amylase), beta amylase (β-Amylase), carbohydrase (Glucoamylase), amylopectase (Debranchins enzyme).Their hydrolysis are to the major glycosides key (α-1 in starch, 4 glycosidic bonds and α-1,6 glycosidic bonds), the Starch Hydrolysis of gelatinization is made to be the residual chain of starch more soluble in water and reducing sugar (glucose, fructose and maltose), then through filtering, eliminate other impurity except starch in sample before hydrochloric acid hydrolysis, avoid other composition such as cellulose, hemicellulose by hydrochloric acid hydrolysis.On the one hand starch is separated from poor unstrained spirits by enzymolysis, purifying is for the sample of hydrochloric acid hydrolysis, and enzymolysis can make starch obtain certain hydrolysis on the other hand, then after digest in conjunction with hydrochloric acid, ensure that the residual chain of starch obtains complete hydrolysis, guarantee the accuracy of testing result.Because AMS can single-minded efficient hydrolyzing alpha-Isosorbide-5-Nitrae glycosidic bond, hydrolyzing alpha-1, the 6-glycosidic bond that amylopectase can be efficiently single-minded, therefore preferably AMS and amylopectase combine, and make amylopectin and amylose can fast hydrolyzing.Regulate when enzyme digestion reaction pH and control temperature in order to reach the optimum reaction conditions of AMS and amylopectase, ensure hydrolysis result.Damping fluid is utilized to regulate can to make after pH pH to remain on a stable scope, to ensure the carrying out of enzyme digestion reaction.AMS is mainly divided into Thermostable α-Amylase and mesophilicα-diastase, and wherein Thermostable α-Amylase optimal reaction temperature is 90 ~ 100 DEG C, and mesophilicα-diastase optimal reaction temperature is 50 ~ 70 DEG C.Mesophilicα-diastase is to metallic ions Ca
2+dependence larger, need to add Ca in enzyme reaction process
2+as stabilizing agent, Thermostable α-Amylase is at Ca
2+when concentration is very low, stability just very well, does not need to add Ca in use
2+deng stabilizing agent.In addition, Thermostable α-Amylase higher than the reaction velocity of mesophilicα-diastase at optimal reactive temperature, therefore, preferably uses Thermostable α-Amylase in the reaction velocity of optimal reactive temperature.
In the present invention, at hydrochloric acid digestion process because hydrochloric acid is volatile, preferably use reflux condenser, boiling water bath refluxes, and both can prevent hydrochloric acid from volatilizing in digestion process, and reflux course makes digestive juice constantly mix simultaneously, contributes to making digestion complete.Should select the hydrochloric acid of suitable concn in digestion process, concentration is too low, is difficult to play digestion, and the too high meeting of concentration causes other strong digestions such as carbonization.Within the scope of suitable concentration of hydrochloric acid, when concentration of hydrochloric acid is lower, return time is long, otherwise return time is short.Normal temperature should be cooled to after digestion with during sodium hydroxide titration.
In the present invention, the mensuration of reducing sugar adopts fehling reagent titration, and according to formulae discovery content of starch after titration, computing formula is:
a1 is the quality of reducing sugar in sample; A2 is the quality of reducing sugar in blank reagent; 0.9 when being with glucose meter reducing sugar, is converted into the reduction coefficient of starch; S is content of starch, represents with %; The quality of the drying grain unstrained spirits that m takes when being and removing fat and soluble sugar, x is the water cut of dry front poor unstrained spirits.When detecting reducing sugar, carry out according to the usual operating conditions of fehling reagent titrimetry and step.Because content of starch after converting represents with number percent, therefore note the conversion of unit when calculating, V1, V2, V3 and V4 all represent volume, they should be converted into identical volume unit; A1, A2 and m all represent quality, they should be converted into identical mass unit.
Below in conjunction with embodiment, the specific embodiment of the present invention is described further.
Embodiment the inventive method compares with Hydrochloric Acid Hydrolysis Method
AMS, purchase producer is Jiangsu Ruiyang Biological Technology Co., Ltd., is Thermostable α-Amylase, 20000U/g, and product specification is RY01002; Amylopectase, buys producer for grinding territory (Shanghai) chemical reagent company limited, 2000U/g.
The inventive method is adopted to detect the content of starch change that Luzhou-flavor is made wine in poor unstrained spirits sweat, fermentation period is 60 days, due to 45 days, later amylon fermenation process was tending towards slow, content of starch tends towards stability, therefore detect data and end the 45th day, every 5 days in the same area sampling of pond, cellar for storing things, detect content of starch in poor unstrained spirits.
In the process of the present invention the content of starch in the poor unstrained spirits of wine brewing is detected, get fresh fermented mash, first adopt oven for drying to constant weight, calculate water cut (x); Take the dry rear poor unstrained spirits of 5g (m), grinding, filter paper parcel is placed in 50mL ether, adopts apparatus,Soxhlet's (standard mouth apparatus,Soxhlet's (150mL)) to carry out lixiviate 6h, with the poor unstrained spirits after the ethanol purge ether lixiviate of 100mL 85%; Poor unstrained spirits after ethanol purge is moved in 250mL beaker, adds 50mL distilled water, boiling water bath heats 30min, make the complete gelatinization of the starch in poor unstrained spirits; Starch after gelatinization is placed in 95 DEG C of water-baths, add the phosphate buffer that 0.05M pH is 7, regulate pH 7.0, add Thermostable α-Amylase solution 10mL (enzyme activity is 10000U), reaction time 1h, is cooled to rapidly 4 DEG C to stop Thermostable α-Amylase reaction in frozen water; Be placed in 55 DEG C of water-baths, add acetic acid-sodium acetate buffer solution that 0.05MpH is 4.5, regulate pH to be 5.0, add amylopectin enzyme solutions 5mL (enzyme activity is 5000U) and react 2h, filter, collect filtrate (V1); Then get enzymolysis filtrate 50mL (V2), add 5mL 6mol/L hydrochloric acid, load onto reflux condenser, reflux 1h in boiling water bath, adds 2 methyl red indicators, be neutralized to neutrality with 5mol/L sodium hydroxide solution after cooling, volumetric flask constant volume 150mL (V3), obtains digestive juice; Get digestive juice 10mL (V4), adopt the Fehling Regent (preparation of fehling reagent: first liquid: the massfraction of NaOH is the solution of 0.1g/mL; Second liquid: the massfraction of copper sulphate is the solution of 0.05g/mL; During use, 4 ~ 5 second drops are entered in 2mL first liquid, uses immediately after mixing.) titration, detect content of reducing sugar in titration sample, calculate content of starch, computing formula is:
a1 is the quality of reducing sugar in sample; A2 is the quality of reducing sugar in blank reagent; 0.9 when being with glucose meter reducing sugar, is converted into the reduction coefficient of starch; S is content of starch, represents with %; The quality of the drying grain unstrained spirits that m takes when being and removing fat and soluble sugar, x is the water cut of dry front poor unstrained spirits.Grain unstrained spirits content of starch situation of change is in table 1.
With Hydrochloric Acid Hydrolysis Method, the content of starch change that Luzhou-flavor is made wine in poor unstrained spirits sweat is detected simultaneously, fermentation period is 60 days, due to 45 days, later amylon fermenation process was tending towards slow, content of starch tends towards stability, therefore detect data and end the 45th day, every 5 days in the same area sampling of pond, cellar for storing things, detect content of starch in poor unstrained spirits.
Hydrochloric Acid Hydrolysis Method: take the fresh poor unstrained spirits of 5g (m), add 20mL 6mol/L hydrochloric acid, load onto reflux condenser, reflux 1h in boiling water bath, adds 2 methyl red indicators, be neutralized to neutrality with 5mol/L sodium hydroxide solution after cooling, volumetric flask constant volume 150mL, filter, collect filtrate, make sample preparation solution (V1); Sample thief Treatment Solution 10mL (V2) again, adopts the Fehling Regent (preparation of fehling reagent: first liquid: the massfraction of NaOH is the solution of 0.1g/mL; Second liquid: the massfraction of copper sulphate is the solution of 0.05g/mL; During use, 4 ~ 5 second drops are entered in 2mL first liquid, uses immediately after mixing.) titration, detect content of reducing sugar in titration sample, finally calculate content of starch, computing formula is:
a1 is the quality of reducing sugar in sample; A2 is the quality of reducing sugar in blank reagent; 0.9 when being with glucose meter reducing sugar, is converted into the reduction coefficient of starch; S is content of starch, represents with %.
In the sweat adopting the inventive method and contrast method to measure, poor unstrained spirits content of starch is in table 1, and in table, difference is the difference of result and the inventive method measurement result using contrast method to measure.
Poor unstrained spirits content of starch in table 1 sweat
| Assay method | 0th day | 5th day | 10th day | 15th day | 20th day |
| The inventive method | 19.41％ | 18.76％ | 16.72％ | 14.08％ | 11.63％ |
| Contrast method | 22.12％ | 21.31％ | 19.26％ | 17.09％ | 14.88％ |
| Difference | 2.71％ | 2.55％ | 2.54％ | 3.01％ | 3.25％ |
| Assay method | 25th day | 30th day | 35th day | 40th day | 45th day |
| The inventive method | 10.88％ | 9.65％ | 9.01％ | 8.85％ | 8.65％ |
| Contrast method | 13.45％ | 12.64％ | 12.06％ | 11.86％ | 11.68％ |
| Difference | 2.57％ | 2.99％ | 3.05％ | 3.01％ | 3.03％ |
Measurement result (see table 1) shows: the content of starch measured through Hydrochloric Acid Hydrolysis Method is all higher.Data as can be seen from table 1, content of starch constantly changes in fermented mash process, and early stage, consumption of starch speed was comparatively slow, and after the 5th day, content of starch starts to decline comparatively fast, and after the 20th day, content of starch decline rate slows down gradually, and tends towards stability.Adopting the present invention institute to survey, content of starch drops to the 45th day gradually from the 0th day 19.41% 8.65%, but the content of starch that employing Hydrochloric Acid Hydrolysis Method is surveyed then exceeds 2.71% and 3.03% on year-on-year basis.This not only Starch Hydrolysis is become reducing sugar owing to adopting in Hydrochloric Acid Hydrolysis Method process, part hemicellulose or poly-pentose are hydrolyzed out simultaneously, so that measured result slightly exceeds about 3% than actual content of starch, nutritional labeling situation of change and the fermentation appearance of poor unstrained spirits cannot be reflected exactly.And the result adopting the inventive method to measure more tallies with the actual situation, content of starch in poor unstrained spirits can accurately be reflected, this for management and Instructing manufacture significant.
Claims (3)
1. the assay method of poor unstrained spirits content of starch, is characterized in that: comprise the following steps:
The process of a, sample: dry poor unstrained spirits, removes fat and soluble sugar, gelatinization; Described removal fat and soluble sugar refer to: take dry rear poor unstrained spirits, ether lixiviate, then the ethanol purge using 100mL 85%; Described ether lixiviate adopts apparatus,Soxhlet's, more than lixiviate 6h;
B, enzymolysis: by adding diastase reaction in the sample after step a gelatinization, filter, collect filtrate, filtrate volume is V1;
C, hydrochloric acid digest: from the filtrate that step b obtains, get the filtrate that volume is V2, add hydrochloric acid, fully digest, be neutralized to neutrality with sodium hydroxide solution, obtain digestive juice, digestive juice volume is V3;
The assay method of d, reducing sugar: get the digestive juice that volume is V4 from the digestive juice that step c obtains, adopts fehling reagent titrimetry, detects content of reducing sugar, and calculate content of starch, computing formula is:
a1 is the quality of reducing sugar in sample; A2 is the quality of reducing sugar in blank reagent; 0.9 when being with glucose meter reducing sugar, is converted into the reduction coefficient of starch; S is content of starch, represents with %; The quality of the drying grain unstrained spirits that m takes when being and removing fat and soluble sugar, x is the water cut of dry front poor unstrained spirits;
Wherein, the diastase described in step b is Thermostable α-Amylase and amylopectase, adopts the mode of enzymolysis step by step, and the first step is that Thermostable α-Amylase reacts, and second step is that amylopectase reacts;
The reaction conditions of Thermostable α-Amylase is as follows: regulate pH to be 6.0 ~ 7.0 with the phosphate buffer that pH is 7.0, and the dry poor unstrained spirits of every 1g uses the Thermostable α-Amylase of more than 2000 Mei Huo units, temperature control 90 ~ 100 DEG C, reaction 1 ~ 2h;
The reaction conditions of amylopectase is as follows: regulate pH to be 4.5 ~ 5.5 with acetic acid-sodium acetate buffer solution that pH is 4.5, and the drying grain unstrained spirits of every 1g uses the amylopectase of more than 1000 Mei Huo units, temperature control 55 ~ 60 DEG C, reaction 2h.
2. the assay method of poor unstrained spirits content of starch according to claim 1, is characterized in that: the drying grain unstrained spirits described in step a refers to: fresh poor unstrained spirits to be measured is placed in baking oven, dries to constant weight.
3. the assay method of poor unstrained spirits content of starch according to claim 1 and 2, it is characterized in that: the gelatinization described in step a refers to: the poor unstrained spirits after removing fat and soluble sugar is dissolved in distilled water, the consumption of distilled water is more than 10 times of dry poor unstrained spirits weight, be heated to 90 ~ 100 DEG C, keep 30min.
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| CN103728269A (en) * | 2014-01-14 | 2014-04-16 | 安徽古井贡酒股份有限公司 | Method for near infrared rapid detection of physical and chemical indicators in wine stock |
| CN104677895B (en) * | 2015-01-26 | 2017-07-25 | 山东省果树研究所 | A kind of method for determining Chinese chestnut content of starch |
| CN105548077B (en) * | 2015-12-07 | 2018-05-15 | 中国水稻研究所 | Utilize the method for total starch content in index determination rice |
| CN106093271A (en) * | 2016-06-03 | 2016-11-09 | 内蒙古蒙牛乳业（集团）股份有限公司 | The assay method of content of reducing sugar in lactose |
| CN107167550A (en) * | 2017-07-07 | 2017-09-15 | 北京农学院 | Non-Structural Carbohydrate content assaying method in silage corn |
| CN109298130A (en) * | 2018-12-26 | 2019-02-01 | 烟台喜旺肉类食品有限公司 | The detection method of starch in a kind of high sugared dried meat floss |
| CN110333329A (en) * | 2019-05-08 | 2019-10-15 | 重庆瑞钛科技有限公司 | The rapid detection method of amylopectin content in wine brewing grain |
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| CN100467606C (en) * | 2006-10-17 | 2009-03-11 | 颜怀伟 | Process for rapid production of fuel alcohol in high yield by two main fermentations of corn comprising enzymatic liquefaction and acid saccharification and medium extraction of alcohol |
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