CN104946537B - A kind of purple ball algae culture medium - Google Patents
A kind of purple ball algae culture medium Download PDFInfo
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- CN104946537B CN104946537B CN201510408149.8A CN201510408149A CN104946537B CN 104946537 B CN104946537 B CN 104946537B CN 201510408149 A CN201510408149 A CN 201510408149A CN 104946537 B CN104946537 B CN 104946537B
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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Abstract
The invention discloses a kind of purple ball algae culture medium, the formula of the purple ball algae culture medium includes the component of following mass concentration：Co(NH2)220 30mg/L, NaH2PO48 12mg/L, ironic citrate 1 2mg/L, Na2EDTA 5 10mg/L, VB10.005 0.007mg/L, VB120.04 0.06mg/L, ZnSO4·4H2O 10 20 μ g/L, MnCl2·4H2O 100 200 μ g/L, CuSO4·5H2O5 10 μ g/L, Na2MoO4·2H2O 5 7.5 μ g/L, CoCl2·6H2O 5‑10μg/L；Also include the 1mg/L of the plant growth regulator Titanium Citrate 0.1 and 1mg/L of lutetium salt 0.1.Culture medium of the present invention has the advantages of growth rate is fast, cell density is big when cultivating purple ball algae, suitable for large-scale production.
Description
Technical field
The present invention relates to a kind of algae media, more particularly to a kind of purple ball algae culture medium.
Background technology
Purple ball algae Porphyridium cruentum category Rhodophyta, former Rhodophyceae, purple ball Cutleriales, Zi Qiu algaes section, purple ball algae
Category, it is that aubergine or peony marine unicellular microalgae is presented in different growth phases in one kind, contains abundant arachidonic acid
The polyunsaturated fatty acid such as AA and eicosapentaenoic acid EPA PUFAs.AA and EPA is the important essential fatty acid of human body, and AA has
Cardiac excitability is adjusted, neuroendocrine is participated in, promote cell division and suppresses the function of platelet aggregation, and EPA is human body
Synthesis of prostaglandins and its derivative prostacyclin, thromboxane, the precursor of leukotriene, blood fat quality can be improved, reduce cholesterol
And blood viscosity and blood sugar concentration, it can effectively treat and prevent the cardiovascular and cerebrovascular diseases such as coronary heart disease and anti-inflammatory, anti-allergic effects.
Therefore there is purple ball algae higher culture to be worth.
At present, subject matter existing for purple ball algae large-scale culture is to grow more slowly, and Biomass yield is relatively low, and closes
Suitable culture medium can greatly facilitate the growth of purple ball algae.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of close beneficial to purple ball algae growth rate, increase cell is improved
Degree, so as to improve culture efficiency, the purple ball algae culture medium particularly suitable for pilot scale culture.
In order to solve the above technical problems, the technical scheme is that：Purple ball algae culture medium, the purple ball algae culture medium
Formula includes the component of following mass concentration：Co(NH2)220-30mg/L, NaH2PO48-12mg/L, ironic citrate 1-2mg/L,
Na2EDTA 5-10mg/L, VB10.005-0.007mg/L, VB120.04-0.06mg/L, ZnSO4·4H2O 10-20 μ g/L,
MnCl2·4H2O 100-200 μ g/L, CuSO4·5H2O 5-10 μ g/L, Na2MoO4·2H2O 5-7.5 μ g/L, CoCl2·
6H2O5-10μg/L；Also include plant growth regulator 0.1-1mg/L and lutetium salt 0.1-1mg/L.
As preferable technical scheme, the formula of the purple ball algae culture medium includes the component of following mass concentration：Co
(NH2)220-30mg/L, NaH2PO48-12mg/L, ironic citrate 1-2mg/L, Na2EDTA7.5mg/L, VB10.006mg/L,
VB120.05mg/L, ZnSO4·4H2O 15 μ g/L, MnCl2·4H2O150 μ g/L, CuSO4·5H2The μ g/L of O 7.5,
Na2MoO4·2H2O 6 μ g/L, CoCl2·6H2O7.5 μ g/L, in addition to plant growth regulator 0.1-1mg/L and lutetium salt
0.1-1mg/L。
Wherein, the plant growth regulator preferably uses Titanium Citrate, and the lutetium salt preferably uses lutecium chloride.
Purple ball algae culture medium of the present invention is beneficial to frustule fast-growth, and the purple ball algae cell density turned out is big, special
It is not due to that with the addition of plant growth regulator Titanium Citrate 0.1-1mg/L and lutetium salt 0.1-1mg/L, formula compatibility is reasonable,
Beneficial to promotion frustule fast breeding so that frustule concentration increases, and is produced suitable for high-volume pilot scale culture.
Embodiment
With reference to specific embodiment, purple ball algae culture medium provided by the invention is described in detail.
Embodiment one：
(1) the purple ball algae culture medium being formulated as follows with nature seawater configuration：Co(NH2)220mg/L, NaH2PO48mg/L,
Ironic citrate 1mg/L, Na2EDTA 7.5mg/L, lutecium chloride 0.1mg/L, Titanium Citrate 0.1mg/L, VB10.006mg/L, VB12
0.05mg/L, ZnSO4·4H2O 15 μ g/L, MnCl2·4H2O 150 μ g/L, CuSO4·5H2O 7.5 μ g/L, Na2MoO4·
2H2O 6 μ g/L, CoCl2·6H2O 7.5μg/L。
(2) the purple ball algae in growth period of taking the logarithm is inoculated into the culture medium and cultivated, and daily illumination 10 hours, illumination is strong
Degree is about 50 μm of ol/ (m2S), case temperature control is trained in 25 degrees centigrades, records purple ball in culture medium at regular intervals
The cell concentration of algae, refer to table 1.
Embodiment two：
(1) the purple ball algae culture medium being formulated as follows with nature seawater configuration：Co(NH2)230mg/L, NaH2PO4 12mg/
L, ironic citrate 2mg/L, Na2EDTA 7.5mg/L, lutecium chloride 1mg/L, Titanium Citrate 1mg/L, VB10.006mg/L, VB12
0.05mg/L, ZnSO4·4H2O 15 μ g/L, MnCl2·4H2O 150 μ g/L, CuSO4·5H2O 7.5 μ g/L, Na2MoO4·
2H2O 6 μ g/L, CoCl2·6H2O 7.5μg/L。
(2) the purple ball algae in growth period of taking the logarithm is inoculated into the culture medium and cultivated, and daily illumination 10 hours, illumination is strong
Degree is about 50 μm of ol/ (m2S), case temperature control is trained in 25 degrees centigrades, records purple ball in culture medium at regular intervals
The cell concentration of algae, refer to table 1.
Comparative example
Using seawater f/2 culture mediums as control medium, the purple ball algae in growth period of taking the logarithm is inoculated into the culture medium
Cultivated, daily illumination 10 hours, intensity of illumination is about 50 μm of ol/ (m2S), case temperature control is trained on 25 degrees Celsius of left sides
The right side, the cell concentration of purple ball algae in culture medium is recorded at regular intervals.
Comparative example is contrasted with embodiment one, embodiment two, and the result of contrast is as shown in table 1：
Table 1：The cell concentration contrast table of the purple ball algae of different culture media culture
As it can be seen from table 1 purple can be made more quickly using SBM culture mediums using culture medium ratio provided by the present invention
Ball algae breeds, thus the maximum concentration that algae can reach illustrates purple ball provided by the invention also above control medium SBM about 70%
Algae culture medium has the fast-growth of obvious culture advantage, more conducively frustule.
The schematical embodiment of the present invention is the foregoing is only, is not limited to the scope of the present invention.It is any
Those skilled in the art, made equivalent variations and modification on the premise of the design of the present invention and principle is not departed from,
The scope of protection of the invention should be belonged to.
Claims (2)
- A kind of 1. purple ball algae culture medium, it is characterised in that：The formula of the purple ball algae culture medium includes the group of following mass concentration Point：
- 2. purple ball algae culture medium as claimed in claim 1, it is characterised in that：The formula of the purple ball algae culture medium includes following The component of mass concentration：
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105524838A (en) * | 2016-03-07 | 2016-04-27 | 临沂大学 | Method for two-stage culture of porphyridium by using urine as main nitrogen source |
| CN113278091A (en) * | 2021-06-29 | 2021-08-20 | 广东湛江海洋医药研究院 | Porphyridium polysaccharide and preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4417415A (en) * | 1982-04-26 | 1983-11-29 | Battelle Development Corporation | Process for culturing a microalga, and extracting a polysaccharide therefrom |
| CN102557919A (en) * | 2011-12-13 | 2012-07-11 | 福州超大现代农业发展有限公司 | Method for synthesizing citricacide-titatnium chelate by microwave chelation process by using new metatitanic acid precipitate |
| CN104611230A (en) * | 2015-02-03 | 2015-05-13 | 宁波浮田生物技术有限公司 | Nannochloris oculata culture medium |
| CN104726338A (en) * | 2014-11-25 | 2015-06-24 | 临沂大学 | Method for increasing content of arachidonic acid in porphyridium |
-
2015
- 2015-07-13 CN CN201510408149.8A patent/CN104946537B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4417415A (en) * | 1982-04-26 | 1983-11-29 | Battelle Development Corporation | Process for culturing a microalga, and extracting a polysaccharide therefrom |
| CN102557919A (en) * | 2011-12-13 | 2012-07-11 | 福州超大现代农业发展有限公司 | Method for synthesizing citricacide-titatnium chelate by microwave chelation process by using new metatitanic acid precipitate |
| CN104726338A (en) * | 2014-11-25 | 2015-06-24 | 临沂大学 | Method for increasing content of arachidonic acid in porphyridium |
| CN104611230A (en) * | 2015-02-03 | 2015-05-13 | 宁波浮田生物技术有限公司 | Nannochloris oculata culture medium |
Non-Patent Citations (2)
| Title |
|---|
| Biological toxicity of lanthanide elements on algae;Peidong Tai，et al.;《Chemosphere》;20100614;第80卷;摘要，第1032页左栏第1段至第1035页左栏第2段，图1-4，表1 * |
| 紫球藻培养条件优化;王长海 等;《化工冶金》;19990715;第20卷(第3期);第272-277页 * |
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