CN104946537A

CN104946537A - Porphyridium culture medium

Info

Publication number: CN104946537A
Application number: CN201510408149.8A
Authority: CN
Inventors: 王兆伟; 彭小伟; 杜学星; 王弢; 靳立兵; 王哲恩; 王闪
Original assignee: Ningbo Fu Tian Bioisystech Co Ltd
Current assignee: Ningbo Fu Tian Bioisystech Co Ltd
Priority date: 2015-07-13
Filing date: 2015-07-13
Publication date: 2015-09-30
Anticipated expiration: 2035-07-13
Also published as: CN104946537B

Abstract

The invention discloses a porphyridium culture medium. The porphyridium culture medium comprises the following components: by mass concentration, 20-30 mg/L of Co(NH2)2, 8-12 mg/L of NaH2PO4, 1-2 mg/L of ferric citrate, 5-10 mg/L of Na2EDTA, 0.005-0.007 mg/L of VB1, 0.04-0.06 mg/L of VB12, 10-20 micron g/L ofZnO4.4H2O, 100-200 micron g/L of MnCl2.4H2O, 5-10 micron g/L of CuSO4.5H2O, 5-7.5 micron g/L of Na2MoO4.2H20 and 5-10 micron g/L of CoCl2.6H2O, and further comprises 0.1-l mg/L of plant growth regulator citricacide-titatnium chelate and 0.1-l mg/L of lutetium salt. When porphyridium is cultured by using the culture medium, the porphyridium has the advantages that the growth velocity is high and the cell density is large. The porphyridium culture medium is suitable for large scale batch production.

Description

A kind of Porphyridium cruentum substratum

Technical field

The present invention relates to a kind of algae media, particularly relate to a kind of Porphyridium cruentum substratum.

Background technology

Porphyridium cruentum Porphyridium cruentum belongs to rhodophyta, former Rhodophyceae, Porphyridium cruentum order, Porphyridium cruentum section, Porphyridium, that one presents red-purple or the micro-algae of scarlet marine unicellular at Different growth phases, containing polyunsaturated fatty acid PUFAs such as abundant Cytochrome P450 Arachidonic Acid Epoxygenase and timnodonic acid EPA.AA and EPA is the indispensable fatty acid that body weight for humans is wanted, AA has the function regulating cardiac excitability, participate in neuroendocrine, promote cell fission and anticoagulant, and EPA is the precursor of human body synthesis of prostaglandins and derivative prostacyclin thereof, thromboxane, leukotriene, blood fat quality, reduction cholesterol and blood viscosity and blood sugar concentration can be improved, can effectively treat and prevent the cardiovascular and cerebrovascular diseases such as coronary heart disease and anti-inflammatory, anti-allergic effects.Therefore Porphyridium cruentum has higher cultivation value.

At present, the subject matter that Porphyridium cruentum pilot scale culture exists grows comparatively slowly, and Biomass yield is lower, and suitable substratum can promote the growth of Porphyridium cruentum greatly.

Summary of the invention

Technical problem to be solved by this invention is to provide one and is beneficial to raising Porphyridium cruentum growth velocity, increases cell density, thus improves culture efficiency, is particularly suitable for the Porphyridium cruentum substratum of pilot scale culture.

For solving the problems of the technologies described above, technical scheme of the present invention is: Porphyridium cruentum substratum, and the formula of described Porphyridium cruentum substratum comprises the component of following mass concentration: Co (NH ₂) ₂20-30mg/L, NaH ₂pO ₄8-12mg/L, ironic citrate 1-2mg/L, Na ₂eDTA 5-10mg/L, VB ₁0.005-0.007mg/L, VB ₁₂0.04-0.06mg/L, ZnSO ₄4H ₂o 10-20 μ g/L, MnCl ₂4H ₂o 100-200 μ g/L, CuSO ₄5H ₂o 5-10 μ g/L, Na ₂moO ₄2H ₂o 5-7.5 μ g/L, CoCl ₂6H ₂o5-10 μ g/L; Also comprise plant growth regulator 0.1-1mg/L and lutetium salt 0.1-1mg/L.

As preferred technical scheme, the formula of described Porphyridium cruentum substratum comprises the component of following mass concentration: Co (NH ₂) ₂20-30mg/L, NaH ₂pO ₄8-12mg/L, ironic citrate 1-2mg/L, Na ₂eDTA7.5mg/L, VB ₁0.006mg/L, VB ₁₂0.05mg/L, ZnSO ₄4H ₂o 15 μ g/L, MnCl ₂4H ₂o150 μ g/L, CuSO ₄5H ₂o 7.5 μ g/L, Na ₂moO ₄2H ₂o 6 μ g/L, CoCl ₂6H ₂o7.5 μ g/L, also comprises plant growth regulator 0.1-1mg/L and lutetium salt 0.1-1mg/L.

Wherein, described plant growth regulator preferably adopts Titanium Citrate, and described lutetium salt preferably adopts lutecium chloride.

Porphyridium cruentum substratum of the present invention is beneficial to frustule and grows fast; the Porphyridium cruentum cell density of turning out is large; particularly owing to the addition of plant growth regulator Titanium Citrate 0.1-1mg/L and lutetium salt 0.1-1mg/L; formula compatibility is reasonable; be beneficial to and promote frustule fast breeding; frustule concentration is increased, is suitable for pilot scale culture in enormous quantities and produces.

Embodiment

Below in conjunction with specific embodiment, Porphyridium cruentum substratum provided by the invention is described in detail.

Embodiment one:

(1) with the Porphyridium cruentum substratum of the following formula of nature seawater configuration: Co (NH ₂) ₂20mg/L, NaH ₂pO ₄8mg/L, ironic citrate 1mg/L, Na ₂eDTA 7.5mg/L, lutecium chloride 0.1mg/L, Titanium Citrate 0.1mg/L, VB ₁0.006mg/L, VB ₁₂0.05mg/L, ZnSO ₄4H ₂o 15 μ g/L, MnCl ₂4H ₂o 150 μ g/L, CuSO ₄5H ₂o 7.5 μ g/L, Na ₂moO ₄2H ₂o 6 μ g/L, CoCl ₂6H ₂o 7.5 μ g/L.

(2) Porphyridium cruentum of taking the logarithm vegetative period is inoculated in this substratum and cultivates, illumination every day 10 hours, and intensity of illumination is about 50 μm of ol/ (m ²s), training case temperature controls at 25 degrees centigrade, records the cell concn of Porphyridium cruentum in substratum at set intervals, refers to table 1.

Embodiment two:

(1) with the Porphyridium cruentum substratum of the following formula of nature seawater configuration: Co (NH ₂) ₂30mg/L, NaH ₂pO ₄12mg/L, ironic citrate 2mg/L, Na ₂eDTA 7.5mg/L, lutecium chloride 1mg/L, Titanium Citrate 1mg/L, VB ₁0.006mg/L, VB ₁₂0.05mg/L, ZnSO ₄4H ₂o 15 μ g/L, MnCl ₂4H ₂o 150 μ g/L, CuSO ₄5H ₂o 7.5 μ g/L, Na ₂moO ₄2H ₂o 6 μ g/L, CoCl ₂6H ₂o 7.5 μ g/L.

Comparative example

Use seawater f/2 substratum substratum in contrast, the Porphyridium cruentum in vegetative period of taking the logarithm is inoculated in this substratum to be cultivated, illumination every day 10 hours, and intensity of illumination is about 50 μm of ol/ (m ²s), training case temperature controls at 25 degrees centigrade, records the cell concn of Porphyridium cruentum in substratum at set intervals.

Comparative example and embodiment one, embodiment two contrast, and the result of contrast is as shown in table 1:

Table 1: the cell concn contrast table of the Porphyridium cruentum that different culture media is cultivated

As can be seen from Table 1, use substratum provided by the present invention than using SBM substratum to make Porphyridium cruentum breed more quickly, the maximum concentration that algae can reach is also higher than control medium SBM about 70%, illustrate that Porphyridium cruentum substratum provided by the invention has obvious cultivation advantage thus, be more conducive to the quick growth of frustule.

The foregoing is only the schematic embodiment of the present invention, and be not used to limit scope of the present invention.Any those skilled in the art, equivalent variations done under the prerequisite not departing from design of the present invention and principle and amendment, all should belong to the scope of protection of the invention.

Claims

1. a Porphyridium cruentum substratum, is characterized in that: the formula of described Porphyridium cruentum substratum comprises the component of following mass concentration:

2. Porphyridium cruentum substratum as claimed in claim 1, is characterized in that: the formula of described Porphyridium cruentum substratum comprises the component of following mass concentration:

3. Porphyridium cruentum substratum as claimed in claim 1 or 2, is characterized in that: described plant growth regulator is Titanium Citrate.

4. Porphyridium cruentum substratum as claimed in claim 1 or 2, is characterized in that: described lutetium salt is lutecium chloride.