CN104944596A - Method for treating hexavalent chromium wastewater by aspergillus niger spore - Google Patents
Method for treating hexavalent chromium wastewater by aspergillus niger spore Download PDFInfo
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- CN104944596A CN104944596A CN201510309569.0A CN201510309569A CN104944596A CN 104944596 A CN104944596 A CN 104944596A CN 201510309569 A CN201510309569 A CN 201510309569A CN 104944596 A CN104944596 A CN 104944596A
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- aspergillus niger
- niger spore
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Abstract
The invention discloses a method for treating hexavalent chromium wastewater by pretreating fungi spore, and aims to solve the problems that the environment is seriously polluted by the hexavalent chromium wastewater, and human health is influenced. The method comprises the steps of selection and coordination of a fungi culture medium and research of condition and influence factors for treating the hexavalent chromium wastewater. Through the research on the influence factors, the treating effect on the hexavalent chromium wastewater can be effectively improved, the treating efficiency can be greatly improved, and requirements on treating the industrialized hexavalent chromium wastewater can be met. The method for treating the hexavalent chromium wastewater has the advantages of being low in cost, small in equipment input, convenient to operate and the like, has wide market prospects and application values, and is worthy of large-scale popularization and application.
Description
Technical field
The present invention relates to chromate waste water processing technology field, particularly relate to a kind of method of aspergillus niger spore process sexavalent chrome heavy metal wastewater thereby.
Background technology
Chromium is as a kind of important metallic element, and it is one of industrial most widely used metal.Chromate waste water is mainly derived from the industries such as plating, metallurgy, process hides, paint, pigment, printing and dyeing, pharmacy and manufacture special steel thereof.In industrial chromium-containing waste water, chromium mainly exists with chromic form (Cr (VI)).The toxicity of chromium is relevant with the valence state of its existence, and sexavalent chrome is higher than trivalent chromium toxicity 100 times; Because chromium has " three cause " effect, and be easy to permeates cell membranes, cause various acute and chronic disease, therefore, the process problem strategic point of Cr (VI) waste water is to be solved.
At present, the treatment process of hexavalent chromium wastewater mainly contains Physical, chemical method and biological process.Wherein, biological process has the advantages such as investment is little, working cost is low, non-secondary pollution, obtains and develops faster.The application provides a kind of aspergillus niger spore process containing the method for hexavalent chromium wastewater.
According to By consulting literatures, adsorption treatment is carried out to sexavalent chrome and mostly adopts mycelium, adopt the application of sulfur acid pretreatment aspergillus niger spore process hexavalent chromium wastewater to have no report.
Summary of the invention
The invention provides a kind of method of pre-treatment fungal spore process hexavalent chromium wastewater, object is to solve hexavalent chromium wastewater serious environment pollution, affects the problem of HUMAN HEALTH.
The present invention adopts following technical scheme:
The concrete steps of the method for aspergillus niger spore process sexavalent chrome heavy metal wastewater thereby of the present invention are as follows:
(1) aspergillus niger spore is cultivated:
By the substratum prepared, with 8 layers of gauze and the sealing of one deck kraft paper, put in high-pressure steam sterilizing pan, at 115 DEG C, sterilizing 25min, puts into sterilisable chamber and cools for subsequent use;
Aspergillus niger spore is cultivated before using in above-mentioned substratum, cultivates 7-15 days, temperature 25-35 degree Celsius;
(2) aspergillus niger spore pre-treatment:
The sulfuric acid cultivated by aspergillus niger spore through 0.1mol/L-0.5mol/L after collecting enters pre-treatment, soaks concussion 3 hours, filters 50ml washing 2-3 time, 50 degrees Celsius of dry 8-10 hour;
(3) sexavalent chrome heavy metal wastewater thereby is processed:
Pretreated aspergillus niger spore is joined in hexavalent chromium wastewater, the weightmeasurement ratio of aspergillus niger spore and waste water is 0.5-2.5:100g/ml, adjustment wastewater pH is 1-3, and control wastewater temperature is 10-35 DEG C, under mixing speed is 80-140r/min condition, process 3-5 hour.
In step (1), described substratum composed as follows: sucrose 30-40g, NaNO
33.0-4.0g, MgSO
47H
2o0.5-1.0g, KCl0.5-1.0g, FeSO
40.01g, K
2hPO
41.0g, agar 20-30g, distilled water 1000mL.
In step (3), the weightmeasurement ratio of preferred aspergillus niger spore and waste water is 2:100g/ml.
In step (3), preferably wastewater pH is regulated to be 1.5-2.5.
In step (3), preferably controlling wastewater temperature is 30 DEG C.
Positively effect of the present invention is as follows:
The present invention passes through selection and the cooperation of fungi culture medium, process hexavalent chromium wastewater condition and Study on influencing factors.By effectively can improve the treatment effect to hexavalent chromium wastewater to the research of influence factor, significantly promote processing efficiency, meet the requirement of industrial treatment hexavalent chromium wastewater.Process hexavalent chromium wastewater of the present invention, has the advantages such as cost is low, equipment investment is little, easy to operate, has wide market outlook and using value, is worth large-scale promotion, application.
Accompanying drawing explanation
Fig. 1 is absorption prespore scanning electron microscope (SEM) photograph.
Fig. 2 is spore scanning electron microscope (SEM) photograph after absorption.
Embodiment
The following examples describe in further detail of the present invention.
Embodiment 1
The concrete steps of the method for aspergillus niger spore process sexavalent chrome heavy metal wastewater thereby of the present invention are as follows:
(1) aspergillus niger spore is cultivated:
By the substratum prepared, with 8 layers of gauze and the sealing of one deck kraft paper, put in high-pressure steam sterilizing pan, at 115 DEG C, sterilizing 25min, puts into sterilisable chamber and cools for subsequent use;
Aspergillus niger spore is cultivated before using in above-mentioned substratum, cultivates 7 days, temperature 35 degrees Celsius;
(2) aspergillus niger spore pre-treatment:
Aspergillus niger spore is cultivated after collecting and enter pre-treatment through the sulfuric acid of 0.1mol/L-0.5mol/L, soak concussion 3 hours, filter 50ml washing 2-3 time, 50 degrees Celsius of dryings 8 hours;
(3) sexavalent chrome heavy metal wastewater thereby is processed:
Join in hexavalent chromium wastewater by pretreated aspergillus niger spore, the weightmeasurement ratio of aspergillus niger spore and waste water is 0.5:100g/ml, and regulate wastewater pH to be 1, control wastewater temperature is 10-DEG C, under mixing speed is 80-140r/min condition, process 3 hours.
In step (1), described substratum composed as follows: sucrose 30-40g, NaNO
33.0-4.0g, MgSO
47H
2o0.5-1.0g, KCl0.5-1.0g, FeSO
40.01g, K
2hPO
41.0g, agar 20-30g, distilled water 1000mL.
Embodiment 2
The concrete steps of the method for aspergillus niger spore process sexavalent chrome heavy metal wastewater thereby of the present invention are as follows:
(1) aspergillus niger spore is cultivated:
By the substratum prepared, with 8 layers of gauze and the sealing of one deck kraft paper, put in high-pressure steam sterilizing pan, at 115 DEG C, sterilizing 25min, puts into sterilisable chamber and cools for subsequent use;
Aspergillus niger spore is cultivated before using in above-mentioned substratum, cultivates 15 days, temperature 25 degrees Celsius;
(2) aspergillus niger spore pre-treatment:
Aspergillus niger spore is cultivated after collecting and enter pre-treatment through the sulfuric acid of 0.1mol/L-0.5mol/L, soak concussion 3 hours, filter 50ml washing 2-3 time, 50 degrees Celsius of dryings 10 hours;
(3) sexavalent chrome heavy metal wastewater thereby is processed:
Join in hexavalent chromium wastewater by pretreated aspergillus niger spore, the weightmeasurement ratio of aspergillus niger spore and waste water is 2.5:100g/ml, regulates wastewater pH to be 3, and controlling wastewater temperature is 35 DEG C, under mixing speed is 80-140r/min condition, process 5 hours.
In step (1), described substratum composed as follows: sucrose 30-40g, NaNO
33.0-4.0g, MgSO
47H
2o0.5-1.0g, KCl0.5-1.0g, FeSO
40.01g, K
2hPO
41.0g, agar 20-30g, distilled water 1000mL.
Embodiment 3
The concrete steps of the method for aspergillus niger spore process sexavalent chrome heavy metal wastewater thereby of the present invention are as follows:
(1) aspergillus niger spore is cultivated:
By the substratum prepared, with 8 layers of gauze and the sealing of one deck kraft paper, put in high-pressure steam sterilizing pan, at 115 DEG C, sterilizing 25min, puts into sterilisable chamber and cools for subsequent use;
Aspergillus niger spore is cultivated before using in above-mentioned substratum, cultivates 10 days, temperature 30 degrees Celsius;
(2) aspergillus niger spore pre-treatment:
Aspergillus niger spore is cultivated after collecting and enter pre-treatment through the sulfuric acid of 0.1mol/L-0.5mol/L, soak concussion 3 hours, filter 50ml washing 2-3 time, 50 degrees Celsius of dryings 9 hours;
(3) sexavalent chrome heavy metal wastewater thereby is processed:
Join in hexavalent chromium wastewater by pretreated aspergillus niger spore, the weightmeasurement ratio of aspergillus niger spore and waste water is 2:100g/ml, regulates wastewater pH to be 2, and controlling wastewater temperature is 30 DEG C, under mixing speed is 80-140r/min condition, process 4 hours.
In step (1), described substratum composed as follows: sucrose 30-40g, NaNO33.0-4.0g, MgSO
47H
2o0.5-1.0g, KCl0.5-1.0g, FeSO
40.01g, K
2hPO
41.0g, agar 20-30g, distilled water 1000mL.
Comparative example
Joined in waste water by aspergillus niger mycelia and carry out adsorption test, other parameters are with embodiment 3.
Determination of Hexavalent Chromium method:
Cr (VI) adopts diphenyl carbazide spectrophotometry to measure.
The data of the method adsorbing hexavalent chromium described in embodiment 1-3 are as shown in table 1:
Table 1
Method of the present invention is significantly improved relative to the adsorptive capacity tool of traditional mycelia absorption method as can be seen from Table 1.
Fig. 1 is absorption prespore scanning electron microscope (SEM) photograph, and 2 is spore scanning electron microscope (SEM) photographs after absorption.Aspergillus niger spore smooth surface before can finding out absorption from scanning electron microscope, has cavernous structure, and specific surface area is comparatively large, is conducive to the absorption of spore heavy metal.After absorption, after heavy metal, spore scanning electron microscope is visible, surface irregularity, heavy metal sexavalent chrome in absorption.
Although illustrate and describe embodiments of the invention, for the ordinary skill in the art, be appreciated that and can carry out multiple change, amendment, replacement and modification to these embodiments without departing from the principles and spirit of the present invention, scope of the present invention is by claims and equivalents thereof.
Claims (5)
1. a method for aspergillus niger spore process sexavalent chrome heavy metal wastewater thereby, is characterized in that: the concrete steps of described method are as follows:
1. aspergillus niger spore is cultivated:
By the substratum prepared, with 8 layers of gauze and the sealing of one deck kraft paper, put in high-pressure steam sterilizing pan, at 115 DEG C, sterilizing 25min, puts into sterilisable chamber and cools for subsequent use;
Aspergillus niger spore is cultivated before using in above-mentioned substratum, cultivates 7-15 days, temperature 25-35 degree Celsius;
2. aspergillus niger spore pre-treatment:
The sulfuric acid cultivated by aspergillus niger spore through 0.1mol/L-0.5mol/L after collecting enters pre-treatment, soaks concussion 3 hours, filters 50ml washing 2-3 time, 50 degrees Celsius of dry 8-10 hour;
3. sexavalent chrome heavy metal wastewater thereby is processed:
Pretreated aspergillus niger spore is joined in hexavalent chromium wastewater, the weightmeasurement ratio of aspergillus niger spore and waste water is 0.5-2.5:100g/ml, adjustment wastewater pH is 1-3, and control wastewater temperature is 10-35 DEG C, under mixing speed is 80-140r/min condition, process 3-5 hour.
2. the method for aspergillus niger spore process sexavalent chrome heavy metal wastewater thereby as claimed in claim 1, is characterized in that: step 1. in, described substratum composed as follows: sucrose 30-40g, NaNO
33.0-4.0g, MgSO
47H
2o 0.5-1.0g, KCl 0.5-1.0g, FeSO
40.01g, K
2hPO
41.0g, agar 20-30g, distilled water 1000mL.
3. the method for aspergillus niger spore process sexavalent chrome heavy metal wastewater thereby as claimed in claim 1, is characterized in that: step 3. in, the weightmeasurement ratio of aspergillus niger spore and waste water is 2:100g/ml.
4. the method for aspergillus niger spore process sexavalent chrome heavy metal wastewater thereby as claimed in claim 1, is characterized in that: step 3. in, adjustment wastewater pH is 1.5-2.5.
5. the method for aspergillus niger spore process sexavalent chrome heavy metal wastewater thereby as claimed in claim 1, is characterized in that: step 3. in, controlling wastewater temperature is 30 DEG C.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105439292A (en) * | 2015-12-14 | 2016-03-30 | 浙江大学 | Method for removing hexavalent chromium in solution through marine Aspergillus sp. |
| CN106045238A (en) * | 2016-08-12 | 2016-10-26 | 周可可 | Treating apparatus for river contaminated by heavy metal chromium |
| CN111041050A (en) * | 2019-12-03 | 2020-04-21 | 同济大学 | Method for converting hexavalent chromium in tea into trivalent chromium by utilizing food-derived microbial fermentation |
| CN111203183A (en) * | 2020-01-17 | 2020-05-29 | 黑龙江省科学院高技术研究院 | Preparation method of chemically grafted and modified biomass adsorbent |
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2015
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| CN102911875A (en) * | 2012-04-27 | 2013-02-06 | 河南省科学院生物研究所有限责任公司 | Preparation method for multifunctional soil remediation microbial inoculum |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105439292A (en) * | 2015-12-14 | 2016-03-30 | 浙江大学 | Method for removing hexavalent chromium in solution through marine Aspergillus sp. |
| CN105439292B (en) * | 2015-12-14 | 2018-01-16 | 浙江大学 | A kind of method of Cr VI in ocean aspergillus niger removing solution |
| CN106045238A (en) * | 2016-08-12 | 2016-10-26 | 周可可 | Treating apparatus for river contaminated by heavy metal chromium |
| CN111041050A (en) * | 2019-12-03 | 2020-04-21 | 同济大学 | Method for converting hexavalent chromium in tea into trivalent chromium by utilizing food-derived microbial fermentation |
| CN111203183A (en) * | 2020-01-17 | 2020-05-29 | 黑龙江省科学院高技术研究院 | Preparation method of chemically grafted and modified biomass adsorbent |
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