CN104944573B - 一种以葡萄糖为共基质驯化可降解2‑cp活性污泥的方法 - Google Patents
一种以葡萄糖为共基质驯化可降解2‑cp活性污泥的方法 Download PDFInfo
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Abstract
一种以葡萄糖为共基质驯化可降解2‑CP活性污泥的方法,属于微生物水处理领域。本发明采用SBR工艺,接种城市污水处理厂二沉池回流污泥,以葡萄糖为共基质逐步驯化活性污泥降解2‑CP。在此过程中,活性污泥中的微生物群落发生了变化,形成新的微生物群落,降解2‑CP的能力逐步提高。本发明操作性强,污泥驯化效率高,驯化效果好。实验证明经过50个周期的驯化,活性污泥可降解进水中浓度为20mg/L的2‑CP,并且污泥可稳定生长。
Description
技术领域
本发明涉及一种污泥驯化方法,特别是一种以葡萄糖为共基质可降解2-CP的活性污泥的驯化,属于微生物水处理领域。
背景技术
氯酚类化合物是一种典型的难降解、毒性大且具有良好的热稳定性的有机污染物,被美国环保署列位优先控制污染物。氯酚类污染物是一种较难生物降解的有机物,采用活性污泥法处理氯酚类废水需要污泥驯化的过程,使活性污泥中的细菌等微生物适应污水环境。共代谢是一种通过生物方法有效降解难生物降解有机物的新技术,本方法通过添加共基质的方法对活性污泥进行驯化,获得对氯酚具有降解能力的活性污泥。
发明内容
本发明的目的是采用葡萄糖为共基质来驯化活性污泥使其具有降解2-CP的能力,为实现以上目的,包括以下步骤:
(1).从城市污水处理厂取得二沉池回流污泥,污泥取回后先用筛网过滤去除其中杂物,并曝气恢复其活性;
(2).将活性污泥接种至SBR反应器中,接种污泥量与城市污水处理厂的浓度相当,为3000-4000mg/L,污泥负荷为0.12-0.15KgCOD/(KgSS·d)
(3).驯化阶段SBR的运行方式如下:
阶段 | 运行方式 | 运行时间/min |
进水期 | 不曝气,瞬间进水 | 1-2 |
曝气期 | 充分曝气 | 600 |
沉淀期 | 不曝气,静置沉淀 | 30 |
出水期 | 不曝气,虹吸进水,出水 | 8-9 |
优选反应器每天运行两个周期,排水比为50%。
进水采用含2-CP的待处理的废水,碳源采用葡萄糖和2-CP共基质,并添加微生物所必需的其他元素;
驯化阶段每天监测进出水的COD以及2-CP的浓度,COD的去除率保持在85%以上,定期排泥使SBR反应器内污泥浓度为3000-4000mg/L,污泥龄为15-20天;在使得COD去除率保持稳定的基础上不断提高2-CP的进水浓度,直至2-CP降解率达100%,COD降解率达到90%以上,污泥的生长状态稳定。
优选驯化完毕时,2-CP进水浓度为20mg/L时,降解率达100%,COD降解率达到90%以上,并且污泥能够稳定地生长。
驯化过程中添加易降解的共基质葡萄糖,葡萄糖的进水浓度为400-600mg/L,2-CP的起始进水浓度为2-3mg/L,然后不断提高2-CP的进水浓度。
采用上述方法驯化的污泥用于的降解2-CP,在含2-CP的废水中加入葡萄糖,葡萄糖的浓度与驯化时的浓度相当。
本发明的驯化污泥壳完全降解浓度为20mg/L的2-CP。
本发明具有以下有益效果:首先污泥的取材方便,污泥取自城市污水厂。其次对于难降解有机物而言,通过加入易降解有机物,诱导微生物的最大生物氧化率,增强微生物活性,并在反应中产生目标污染物的非专一性酶,进而提高难降解有机物的可生化性。当废水中只有难降解化合物存在时,微生物虽然能降解一定量的该类化合物,但整体活性受到抑制。而后加入的易降解化合物使微生物的活性得以发挥,产生非专一性关键酶,提高难降解化合物的降解效率。本发明向废水中添加易生物降解的葡萄糖,可显著改善废水的可生化性。其重要的原因是,易降解的葡萄糖与难降解有机物被微生物共代谢作用所利用,一些工业含有氯酚废水由于缺乏易降解的诱导基质,有机物的生物降解难以进行,本发明用过补充碳源的方式促进共代谢的发生,提高氯酚的降解性。
附图说明
附图1为本发明实施例1驯化阶段进出水COD浓度变化图;
附图2为本发明实施例1驯化阶段进出水2-CP浓度变化图。
具体实施方式
下面实施例将结合附图对本发明作进一步的说明,但本发明并不限于以下实施例。
实施例1:
1.(1).从城市污水处理厂取得二沉池回流污泥,污泥取回后先用筛网过滤去除其中杂物,并曝气恢复其活性。
(2).将活性污泥接种至SBR反应器中,接种污泥量与城市污水处理厂的浓度相当,为3000-4000mg/L,污泥负荷为0.12-0.15KgCOD/(KgSS·d)
(3).SBR的运行方式如下:
阶段 | 运行方式 | 运行时间/min |
进水期 | 不曝气,瞬间进水 | 1-2 |
曝气期 | 充分曝气 | 600 |
沉淀期 | 不曝气,静置沉淀 | 30 |
出水期 | 不曝气,虹吸进水,出水 | 8-9 |
排水比为50%。
反应器每天运行两个周期,进水采用含有2-CP的模拟废水,碳源采用葡萄糖和2-CP共基质,并添加其他的微生物所需元素,葡萄糖的浓度为500mg/L。
(4)驯化阶段每天监测进出水的COD以及2-CP的浓度,COD的去除率保持在90%左右,定期排泥使污泥浓度维持在3000-4000mg/L,污泥龄在15-20天。在COD去除率保持稳定的基础上不断提高2-CP的进水浓度,具体见图2。驯化期间出现短暂的2-CP去除率下降的现象,维持2-CP进水浓度一段时间后,去除率回升。
经过五十天左右的驯化,当进水中2-CP的浓度为20mg/L,降解率可达100%,COD降解率达到90%以上,并且污泥能够稳定地生长,即可认为驯化完毕。
然后进行待处理的含有2-CP的模拟污水的处理,2-CP的浓度为20mg/L,污水中加入葡萄糖,葡萄糖的浓度为500mg/L,污水处理效果是2-CP 100%降解。
Claims (4)
1.一种以葡萄糖为共基质驯化可降解2-CP活性污泥的方法,其特征在于,包括以下步骤:
(1).从城市污水处理厂取得二沉池回流污泥,污泥取回后先用筛网过滤去除其中杂物,并曝气恢复其活性;
(2).将活性污泥接种至SBR反应器中,接种污泥量与城市污水处理厂的浓度相当,为3000-4000mg/L,污泥负荷为0.12-0.15KgCOD/(KgSS·d);
(3).驯化阶段SBR的运行方式如下:
碳源采用葡萄糖和2-CP共基质,并添加微生物所必需的其他元素;
驯化阶段每天监测进出水的COD以及2-CP的浓度,COD的去除率保持在85%以上,定期排泥使SBR反应器内污泥浓度为3000-4000mg/L,污泥龄为15-20天;在使得COD去除率保持稳定的基础上不断提高2-CP的进水浓度,直至2-CP降解率达100%,COD降解率达到90%以上,污泥的生长状态稳定;
葡萄糖的进水浓度为400-600mg/L;2-CP的起始进水浓度为2-3mg/L,然后不断提高2-CP的进水浓度。
2.根据权利要求1所述的一种以葡萄糖为共基质驯化可降解2-CP活性污泥的方法,其特征在于,SBR每天运行两个周期,每个周期排水比为50%。
3.按照权利要求1所述的方法得到的可降解2-CP活性污泥的应用,用于降解2-CP。
4.按照权利要求1所述的方法得到的可降解2-CP活性污泥用于降解2-CP的方法,其特征在于,含2-CP的废水中加入葡萄糖,葡萄糖的浓度与驯化时的浓度相当。
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