CN104931565A - Preparation of screen-printed electrode aptamer sensor for detecting tetracycline residue - Google Patents
Preparation of screen-printed electrode aptamer sensor for detecting tetracycline residue Download PDFInfo
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- CN104931565A CN104931565A CN201510316616.4A CN201510316616A CN104931565A CN 104931565 A CN104931565 A CN 104931565A CN 201510316616 A CN201510316616 A CN 201510316616A CN 104931565 A CN104931565 A CN 104931565A
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Abstract
The invention relates to preparation of a screen-printed electrode aptamer sensor for detecting tetracycline residue. The preparation comprises the following steps: modifying the surface of a screen-printed electrode with a multiwall carbon-chitosan compound and a nano iron tetroxide-chitosan compound layer by layer, and placing at the room temperature till the surface dries; fixing a tetracycline aptamer on the surface of the screen-printed electrode modified with the nano material; carrying out electrochemical test on a prepared tetracycline aptamer sensor; measuring the residue concentration of tetracycline through the current variable quantity of the adtamer sensor caused before and after the combination of the adtamer and tetracycline; obtaining the tetracycline concentration in a milk sample solution. According to the preparation and detection method of the tetracycline aptamer sensor based on the screen-printed electrode provided by the invention, the problem that conventional electrode pretreatment is complex in procedures is well solved; electrodes of the same batch are good in identity, and can be produced in batches; commercialization can be achieved.
Description
Technical field
The present invention relates to a kind of screen printing electrode aptamer sensor preparation detected for tetracycline residue, belong to agricultural product security detection technique field.
Background technology
In recent years, along with developing rapidly of China's livestock breeding industry, milk yield increases substantially, and fresh milk and dairy produce have become the important component part in the people (especially old man and children) life food.Microbiotic is widely used in Prevention and Curation cow disease, because frequent, overdose use, makes to there is a certain amount of antibiotic residue in the animal-derived foods such as milk.Drink for a long time or edible cow's milk or the dairy products having antibiotic residue, be also just equivalent to the absorption microbiotic of long-term low dose, thus the normal flora in human body intestinal canal is suppressed, cause pathogenic bacteria amount reproduction to cause whole body or local infection.Because the mastadenitis of cow incidence of disease is higher, usually treat the obstetric conditions such as mastitis still comparatively general with Fourth Ring, this easily causes the residual of in milk tetracycline.Excessive use TCs inevitably makes the associated antibiotics such as parent metabolic product residue in the muscle of animal, egg, milk, organs and tissues, and then affects health by food chain.Containing antibiotic residue in milk, not only very large harm is caused to the health of people, and heavy economic losses is brought to Dairy Processing enterprise.Therefore strictly must control Residue of Antibiotics in Milk, raise except science will be carried out, manage with delicacy, correctly milk and except prevent disease, also want the antibiotic use of specification.Therefore to avoiding the generation of above-mentioned situation, except controlling from source except antibiotic use, detecting that the antibiotic residue in animal-derived food is the effective way ensureing livestock product safety in time, exactly.Visible, strengthen the detection to antibiotic residue in the agricultural product such as milk, especially ensure that human health has very profound significance.
Traditional method for antibiotic residue detection mainly contains: gas chromatography (GC), high performance liquid chromatography (HPLC), Chromatography/Mass Spectrometry coupling technique (GC/LC-MS), capillary electrophoresis (CE), fluorescence analysis, euzymelinked immunosorbent assay (ELISA) (ELISA).Although these method selectivity are good, highly sensitive, accuracy is high, detection limit is low, can detect multiple element or compound, but it needs expensive instrument and equipment, sample pretreatment process is loaded down with trivial details, time-consuming simultaneously, and require very high to the technical merit of analyst, be unsuitable for field quick detection.Aptamer (Aptamer) is the nucleotide sequence of Prof. Du Yucang, and aptamer and target molecule through repeatedly screening acquisition have very high specificity and affinity.And aptamers is obtained by in-vitro screening and amplification, does not need immune animal or cultured cell, has fabulous accuracy and repeatability, very high purity, can avoid producing differences between batches.In addition, aptamer non-immunogenicity, can under different temperatures, different ions concentration, metal-chelator existence condition denature and renature repeatedly, in building-up process, various reporter molecules (fluorescein or biotin) and function group can accurately be combined on aptamers site.The technology that current antibody carries out diagnosis and detection almost can all be replaced by aptamers.Microbiotic is small haptens material, is difficult to prepare corresponding antibody, even if prepare corresponding antibody, the signal that also there is immunosensor is weak, reusability is poor, reappearance can not meet the problems such as the requirement that actual sample detects.Aptamer just can well address this problem.Compared with traditional analytical approach, aptamers has following features: the selectivity that (1) is higher, does not therefore need to be separated tested component, namely need not carry out pre-service to sample.(2) structure is simple, and volume is little, easy to use, particularly portable immunosensor, is very beneficial for the Fast Measurement of agricultural product security quality.(3) can continuous on-line detection be realized, make the quality control of food processing process become easy.(4) fast response time, amount of samples is few, and compared with other large-sized analytic instruments, immunosensor cost of manufacture is low, and can Reusability.
The object of the invention is to overcome the deficiency that above-mentioned prior art exists, provide that a kind of structure is simple, easy to operate, cost is low and the screen printing electrode aptamer sensor preparation method that detection sensitivity is high.
Summary of the invention
Its technical scheme is: a kind of screen printing electrode aptamer sensor preparation detected for tetracycline residue, it is characterized in that: on the screen printing electrode surface by many walls carbon-shitosan/nanometer four iron oxide-chitosan-modified, drip and be coated with tetracycline aptamers, prepare tetracycline aptamer sensor, tetracycline aptamer sensor is used for the field quick detection of tetracycline residue in milk.
For realizing above function, a kind of screen printing electrode aptamer sensor preparation method for tetracycline residue detection of the present invention is as follows: the preparation of (1) many walls carbon-shitosan (MWCNTs-CHIT) compound: added in 100mL water by 1mL acetic acid standard solution and make 1.0% acetic acid.In the beaker filling 100mL 1.0% acetic acid, add the shitosan of 0.5g, till at room temperature constantly the shitosan stirred in the middle of beaker dissolves completely, obtain chitosan solution for subsequent use.Get 10mL gained chitosan solution again, again many walls of 2.5mg carbon is dissolved in the chitosan solution of 0.1wt% of above-mentioned preparation, in ultrasonic cleaning instrument, ultrasonic 2h is till solution presents uniform and stable state, many walls carbon-shitosan (MWCNTs-CHIT) compound; (2) preparation of nanometer four iron oxide-chitosan complexes: get gained chitosan solution when 10mL prepares many walls carbon-shitosan (MWCNTs-CHIT) compound, again the nano ferriferrous oxide of 2.5mg is dissolved in the chitosan solution of 0.1% of above-mentioned preparation, be constantly stirred to the dissolution homogeneity of gained stable till; (3) preparation of getting many walls carbon-shitosan (MWCNTs-CHIT) compound that 15 μ L prepare is dripped and is coated in the good screen printing electrode surface of pre-service, air drying, and rinse electrode surface with the phosphate buffer of pH7.5, nitrogen dries up; (4), after electrode surface dries, 15 μ L nanometer four iron oxide-shitosan (Fe are got
3o
4-CHIT) compound drips and is coated in electrode surface, leave standstill to drying under normal temperature, and then use ultrapure water electrode surface, nitrogen dries up; (5) then the tetracycline aptamers of 15 μ L 5mM is dropped in above-mentioned on nanometer-material-modified good screen printing electrode, after 4h with ultrapure water surface, dry under being kept at 4 DEG C of conditions for subsequent use.
Described a kind of screen printing electrode aptamer sensor preparation detected for tetracycline residue, it is characterized in that: screen printing electrode cleaning and activation, the structure of aptamer sensor sensitive interface and process characterize (prepares many walls carbon-shitosan and nanometer four iron oxide-chitosan complexes, the synergy of many walls carbon, shitosan and tri-iron tetroxide is utilized jointly to modify screen printing electrode), the foundation of aptamer sensor working curve, the detection of aptamer sensor performance, aptamer sensor is to the detection of actual sample.
Described a kind of screen printing electrode aptamer sensor preparation detected for tetracycline residue, is characterized in that: the optimization of experiment condition, mainly comprises aptamers concentration, the pH testing end liquid and test duration and temperature; The working curve of prepared aptamer sensor is: Δ I (μ A)=1.0643LogC (M)+11.229(R
2=0.9651); Aptamer sensor Performance Detection comprises reappearance, stability, reproducibility, specificity and the aptamer sensor mensuration to the milk sample recovery.
Its preparation principle is: aptamers biology sensor is using aptamers as recognition component, by immobilization technology, aptamers is attached to susceptor surface, after aptamers is combined with object, the compound of formation is associated with the physics of generation or chemical signal, by transducer be translated into relevant with test substance concentration (or activity) can quantitative or accessible physiochemical signal, amplified by secondary instrument again and output signal, thus realizing the detection to test substance.The present invention adopts many walls carbon-shitosan and nanometer four iron oxide-chitosan complexes modified electrode.Many walls carbon-chitosan complexes not only can accelerate the transmission of electronics in electron surface as molecular wire as the ground floor material of modified electrode.Shitosan belongs to polysaccharide, it has excellent film forming, adsorbability, gas penetration potential and perviousness, have good adsorbability, stability and good biocompatibility after film forming, its abundant amino, cellular structure make it be widely used in the preparation of the fixing of biomolecule and modified electrode.The ferric ion of nanometer four iron oxide can promote electrode and the direct electron transmission of test end liquid, and the secondary realizing signal amplifies.And the good biocompatibility of shitosan, for aptamers fixedly provides a good bioelectric interface at electrode surface, the biologically active maintaining aptamers is to be used for and tetracycline effectively combines.Adopt aptamer sensor preparation and the detection method of tetracycline residue in a kind of milk of the present invention, can before milk listing, carry out the Fast Measurement of tetracycline residue, directly whether Tetracycline Residues is exceeded standard and detect, the accumulation causing tetracycline in human body because of edible milk containing tetracycline is avoided to cause serious consequence, for livestock products safety in production and consumption provide the technical support of residue detection.
The present invention's screen printing carbon electrode used makes simple, have and can realize batch production, electrode is integrated, amount of samples is little, cost is low and once uses droppable advantage, avoid cross jamming problem during the multiple sample of shared same electrode detection, for biology sensor move towards cheap, large-scale application provides the promising approach of a kind of tool, solve the pretreated complicated procedures of front electrode well, and with a batch electrode, there is good homogeneity, can produce in batches, realize commercialization.
Described screen printing carbon electrode, comprise the substrate of printed electrode, be printed on on-chip external insulation and at least two contact conductors, substrate is printed with three electrodes, be respectively a working electrode, one to electrode and a contrast electrode, each electrode pair should be connected with a contact conductor.
Described cleaning, activation screen printing carbon electrode, process is: first, screen printing carbon electrode is placed in NaOH solution ultrasonic, ultrapure water, N
2dry up, secondly, electrode is placed in HCl solution ultrasonic, ultrapure water, N
2dry up, again, rinse electrode with absolute ethyl alcohol, N
2dry up, finally, sweep current-time curve in phosphate buffer, scan cycle volt-ampere curve is until stable performance afterwards.
Described a kind of screen printing electrode aptamer sensor preparation detected for tetracycline residue, is characterized in that: to naked screen printing electrode and and the MWCNTs-CHIT electrode, the MWCNTs-CHIT/Fe that modify
3o
4-CHIT composite modified electrode, and after compound modified electrode, fixed adaptation body, aptamer sensor are carried out cyclic voltammetric (CV) in conjunction with the progressively modification after tetracycline and are characterized.Cyclic voltammetry is at-0.2 ~ 0.6V, carries out under sweeping the condition that speed is 0.05V/s, and its test end liquid is for containing 5mmol/L [Fe (CN)
6]
3-/4-with the phosphate buffered solution of the 0.1mol/L pH7.5 of the mixed liquor of 0.1mol/L KCl.At room temperature characterized by AC impedance method (EIS), variable voltage is 5mV, and frequency range is 0.1Hz ~ 100kHz.The residual concentration of tetracycline is combined front and back by aptamers with tetracycline and causes aptamer sensor current change quantity (I=I
1i
2)) measure, wherein I
1the electric current in end liquid is being tested, I for aptamer sensor and object to be measured react front
2represent after modified electrode and object to be measured react and testing the electric current in end liquid.
For reaching above object, following technical scheme is taked to realize: a kind of screen printing electrode aptamer sensor preparation detected for tetracycline residue, it is characterized in that: the cleaning of naked screen printing electrode before the preparation of (1) aptamer sensor, activation and performance test, if the spike potential difference in test loop volt-ampere curve is at below 80mV, oxidation peak and reduction peak symmetry, then described screen printing electrode can use, otherwise will return in cleaning step, until meet the requirements.(2) cleaned glassy carbon electrode surface is dripped and is coated with finely dispersed many walls carbon-chitosan complexes dispersion liquid, after drying, drips and is coated with nanometer four iron oxide-shitosan, then fix tetracycline aptamers.After aptamer sensor preparation terminates, to put in refrigerator 4 DEG C and save backup.
The preparation technology of described aptamer sensor is as follows: drip many walls carbon-shitosan (MWCNTs-CHIT) compound that painting 15 μ L prepares on the screen printing electrode surface that pre-service is good, air drying, with the phosphate buffer of pH7.5 rinse electrode surface many walls carbon-chitosan-modified electrode (MWCNTs-CHIT/GCE); Then on MWCNTs-CHIT/GCE electrode, painting 15 μ L nanometer four iron oxide-shitosan (Fe is dripped
3o
4-CHIT) compound drips and is coated in electrode surface, leave standstill to drying under normal temperature, and then use ultrapure water electrode surface, nitrogen dries up; Finally the tetracycline aptamers of 15 μ L 5mM is dropped in above-mentioned on nanometer-material-modified good screen printing electrode, with ultrapure water surface after 4h, dry and be kept at 4
dEG Cfor subsequent use under condition.
Accompanying drawing explanation
The empty screen printing electrode of electrochemical Characterization (a) of Fig. 1 aptamer sensor assembling process, b screen printing electrode that () has modified is fixed the cyclic voltammetry curve after tetracycline aptamers contact tetracycline, c screen printing electrode that () has modified is fixed the cyclic voltammetry curve after tetracycline aptamers, the screen printing electrode of (d) many walls carbon-chitosan-modified, the cyclic voltammetry curve of the screen printing electrode of (e) many walls carbon-shitosan/tri-iron tetroxide-chitosan-modified.All electrochemical measurements are all at 5mmol/L [Fe (CN)
6]
3-/4-complete with under the phosphate buffered solution of the 0.1mol/L pH7.5 of the mixed liquor of 0.1mol/L KCl.
The typical curve of Fig. 2 aptamer sensor.
The recovery of standard addition of Fig. 3 milk actual sample.
Embodiment
Embodiment: the preparation of (1) many walls carbon-shitosan (MWCNTs-CHIT) compound: 1mL acetic acid standard solution is added in 100mL water and makes 1.0% acetic acid; In the beaker filling 100mL 1.0% acetic acid, add the shitosan of 0.5g, till at room temperature constantly the shitosan stirred in the middle of beaker dissolves completely, obtain chitosan solution for subsequent use; Get 10mL gained chitosan solution again, again many walls of 2.5mg carbon is dissolved in the chitosan solution of 0.1wt% of above-mentioned preparation, in ultrasonic cleaning instrument, ultrasonic 2h is till solution presents uniform and stable state, many walls carbon-shitosan (MWCNTs-CHIT) compound; (2) preparation of many walls carbon-shitosan/nanometer four iron oxide-chitosan complexes: get gained chitosan solution when 10mL prepares many walls carbon-shitosan (MWCNTs-CHIT) compound, again the nano ferriferrous oxide of 2.5mg is dissolved in the chitosan solution of 0.1% of above-mentioned preparation, be constantly stirred to the dissolution homogeneity of gained stable till; (3) cleaning of screen printing electrode: cleaning, activation screen printing carbon electrode, process is: first, screen printing carbon electrode is placed in NaOH solution ultrasonic, ultrapure water, N
2dry up, secondly, electrode is placed in HCl solution ultrasonic, ultrapure water, N
2dry up, again, rinse electrode with absolute ethyl alcohol, N
2dry up, finally, sweep current-time curve in phosphate buffer, scan cycle volt-ampere curve is until stable performance afterwards; (4) activation of screen printing electrode: sweep current-time curve 300s in pH5.0 phosphate buffer, scan cycle volt-ampere curve is until stable performance afterwards; (5) preparation of getting many walls carbon-shitosan (MWCNTs-CHIT) compound that 15 μ L prepare is dripped and is coated in the good screen printing electrode surface of pre-service, air drying, and rinse electrode surface with the phosphate buffer of pH7.5, nitrogen dries up; (6), after electrode surface dries, 15 μ L nanometer four iron oxide-shitosan (Fe are got
3o
4-CHIT) compound drips and is coated in electrode surface, leave standstill to drying under normal temperature, and then use ultrapure water electrode surface, nitrogen dries up; (7) then the tetracycline aptamers of 15 μ L 5mM is dropped in above-mentioned on nanometer-material-modified good electrode, after 4h with ultrapure water surface, dry under being kept at 4 DEG C of conditions for subsequent use; (8) pre-service is carried out to milk: the ratio of milk according to 1:10 is diluted, then centrifugal 90min under 30000 revolutions per seconds (rpm), we obtain middle one deck does not like this have fat and caseic milk whey, is used for subsequent detection with milk whey as sample; (9) painting 15 μ L is dripped containing 5mmol/L [Fe (CN) on the screen printing electrode surface fixing tetracycline aptamers
6]
3-/4-with liquid at the bottom of the test of the mixed liquor of 0.1mol/L KCl, at-0.2 ~ 0.6V, test under sweeping the condition of speed for 0.05V/s; (10) this test tetracycline concentration gradient is 1 × 10
-9m, 1 × 10
-8m, 1 × 10
-7m, 1 × 10
-6m, 1 × 10
-5m, 1 × 10
-4m, 1 × 10
-3m, 1 × 10
-2the tetracycline standard solution of M, the above-mentioned aptamer sensor prepared is detected respectively the tetracycline standard solution of variable concentrations, hatch 14min at normal temperatures, before and after detection reaction, curent change obtains its working curve; (11) residual concentration of tetracycline causes aptamer sensor current change quantity (I=I before and after being combined with tetracycline by aptamers
1i
2)) measure, wherein I
1the electric current in end liquid is being tested, I for aptamer sensor and object to be measured react front
2represent after modified electrode and object to be measured react and testing the electric current in end liquid, the working curve of prepared aptamer sensor is: Δ I (μ A)=1.0643LogC (M)+11.229(R
2=0.9651); (12) it is 92% ~ 98% that the method utilizing mark-on to reclaim detects the recovery of tetracycline in milk sample.
A kind of screen printing electrode aptamer sensor preparation detected for tetracycline residue of the present invention, operating procedure is simple, detection time is shorter, can antibiotic residue in field quick detection milk, have highly sensitive, good stability, high repeatability and other advantages, meet China's antibiotic residue Fast Detection Technique development and internationalization requirement.
Claims (4)
1. the screen printing electrode aptamer sensor preparation detected for tetracycline residue, it is characterized in that: leave standstill to drying by many walls carbon-chitosan complexes, nanometer four iron oxide-chitosan complexes under successively modifying screen printing electrode surface normal temperature, then tetracycline aptamers is fixed on above-mentioned nanometer-material-modified screen printing electrode; The tetracycline aptamer sensor prepared is put into containing 5mmol/L [Fe (CN) 6]
3-/4-test with the phosphate buffered solution of the 0.1mol/L pH7.5 of the mixed liquor of 0.1mol/L KCl, cyclic voltammetry is at-0.2 ~ 0.6V, carry out under sweeping the condition that speed is 0.05V/s, the residual concentration of tetracycline is combined front and back by aptamers with tetracycline and causes aptamer sensor current change quantity to be measured; According to the relation curve between acquired tetracycline concentration and curent change, obtain the tetracycline concentration in milk sample liquid; The preparation technology of described aptamer sensor is as follows: drip many walls carbon-chitosan complexes that painting 15 μ L prepares on the screen printing electrode surface that pre-service is good, air drying, with the phosphate buffer of pH7.5 rinse electrode surface many walls carbon-chitosan-modified electrode; Then on many walls carbon-chitosan-modified electrode, drip a painting 15 μ L nanometer four iron oxide-chitosan complexes drip and be coated in electrode surface, leave standstill under normal temperature to drying, then use ultrapure water electrode surface, nitrogen dries up; Finally the tetracycline aptamers of 15 μ L 5mM is dropped in above-mentioned on nanometer-material-modified good screen printing electrode, with ultrapure water surface after 4h, dry under being kept at 4 DEG C of conditions for subsequent use.
2. a kind of screen printing electrode aptamer sensor preparation detected for tetracycline residue as claimed in claim 1, it is characterized in that: the preparation method of many walls carbon-chitosan complexes is as follows: 1mL acetic acid standard solution is added in 100mL water and makes 1.0% acetic acid, the shitosan of 0.5g is added in the beaker filling 100mL 1.0% acetic acid, till at room temperature constantly the shitosan stirred in the middle of beaker dissolves completely, obtain chitosan solution for subsequent use, get 10mL gained chitosan solution, again many walls of 2.5mg carbon is dissolved in the chitosan solution of 0.1wt% of above-mentioned preparation, in ultrasonic cleaning instrument, ultrasonic 2h is till solution presents uniform and stable state, many walls carbon-chitosan complexes.
3. a kind of screen printing electrode aptamer sensor preparation detected for tetracycline residue as claimed in claim 1, it is characterized in that: the preparation method of nanometer four iron oxide-chitosan complexes is as follows: get gained chitosan solution when 10mL prepares many walls carbon-chitosan complexes, again the nano ferriferrous oxide of 2.5mg is dissolved in the chitosan solution of 0.1% of above-mentioned preparation, be constantly stirred to the dissolution homogeneity of gained stable till.
4. a kind of screen printing electrode aptamer sensor preparation detected for tetracycline residue as claimed in claim 1, it is characterized in that: the cyclic voltammetry of the aptamer sensor of preparation is at-0.2 ~ 0.6V, carry out under sweeping the condition that speed is 0.05V/s, its test end liquid is for containing 5mmol/L [Fe (CN)
6]
3-/4-with the phosphate buffered solution of the 0.1mol/L pH7.5 of the mixed liquor of 0.1mol/L KCl, the residual concentration of tetracycline is combined front and back by aptamers with tetracycline and causes aptamer sensor current change quantity to be measured; The working curve of prepared aptamer sensor is: Δ I (μ A)=1.0643LogC (M)+11.229(R
2=0.9651), according to the relation curve between acquired tetracycline concentration and curent change, the tetracycline concentration in milk sample liquid is obtained.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105353003A (en) * | 2015-10-19 | 2016-02-24 | 山东理工大学 | Microarray electrode-based detection method for antibiotic residues |
| CN105424787A (en) * | 2015-11-20 | 2016-03-23 | 济南大学 | Preparation method of silk screen printing sensor for detecting tetracycline and oxytetracycline at same time |
| CN106596648A (en) * | 2016-12-19 | 2017-04-26 | 宁海县浙工大海洋研究院 | Preparation method of bacterial biosensor for antibiotic detection analysis in environment |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102608189A (en) * | 2012-03-30 | 2012-07-25 | 山东理工大学 | Method for manufacturing nanometer magnetic ferroferric oxide modified immunosensor |
| CN103257175A (en) * | 2013-03-13 | 2013-08-21 | 济南大学 | Preparation method and application of sensor for simultaneously detecting four aminoglycoside antibiotics |
| CN104267078A (en) * | 2014-10-09 | 2015-01-07 | 无锡百灵传感技术有限公司 | Electrochemical sensor for quickly detecting melamine |
| CN104634843A (en) * | 2015-03-02 | 2015-05-20 | 淄博瑞谷自动化控制设备有限公司 | Preparation method and application of sensor based on screen-printed carbon electrode |
-
2015
- 2015-06-11 CN CN201510316616.4A patent/CN104931565A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102608189A (en) * | 2012-03-30 | 2012-07-25 | 山东理工大学 | Method for manufacturing nanometer magnetic ferroferric oxide modified immunosensor |
| CN103257175A (en) * | 2013-03-13 | 2013-08-21 | 济南大学 | Preparation method and application of sensor for simultaneously detecting four aminoglycoside antibiotics |
| CN104267078A (en) * | 2014-10-09 | 2015-01-07 | 无锡百灵传感技术有限公司 | Electrochemical sensor for quickly detecting melamine |
| CN104634843A (en) * | 2015-03-02 | 2015-05-20 | 淄博瑞谷自动化控制设备有限公司 | Preparation method and application of sensor based on screen-printed carbon electrode |
Non-Patent Citations (3)
| Title |
|---|
| LING ZHOU 等: "Electrochemical aptasensor for the detection of tetracycline with multi-walled carbon nanotubes amplification", 《SENSORS AND ACTUATORS B: CHEMICAL》 * |
| XIA SUN 等: "AMPEROMETRIC IMMUNOSENSOR BASED ON GOLD NANOPARTICLES/Fe3O4-FCNTS-CS COMPOSITE FILM FUNCTIONALIZED INTERFACE FOR CARBOFURAN DETECTION", 《ANALYTICAL LETTERS》 * |
| YEMIN GUO 等: "Electrochemical Aptasensor Based on Multiwalled Carbon Nanotubes and Graphene for Tetracycline Detection", 《IEEE SENSORS JOURNAL》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105353003A (en) * | 2015-10-19 | 2016-02-24 | 山东理工大学 | Microarray electrode-based detection method for antibiotic residues |
| CN105424787A (en) * | 2015-11-20 | 2016-03-23 | 济南大学 | Preparation method of silk screen printing sensor for detecting tetracycline and oxytetracycline at same time |
| CN105424787B (en) * | 2015-11-20 | 2017-08-15 | 济南大学 | It is a kind of while detecting the preparation method of the silk-screen printing sensor of tetracycline and terramycin |
| CN106596648A (en) * | 2016-12-19 | 2017-04-26 | 宁海县浙工大海洋研究院 | Preparation method of bacterial biosensor for antibiotic detection analysis in environment |
| CN106596648B (en) * | 2016-12-19 | 2019-02-01 | 宁海县浙工大海洋研究院 | Preparation method for the bacterium living beings sensor that antibiotic in environment tests and analyzes |
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