CN104928763A - Flax degumming liquid and preparation method thereof - Google Patents
Flax degumming liquid and preparation method thereof Download PDFInfo
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- CN104928763A CN104928763A CN201510271462.1A CN201510271462A CN104928763A CN 104928763 A CN104928763 A CN 104928763A CN 201510271462 A CN201510271462 A CN 201510271462A CN 104928763 A CN104928763 A CN 104928763A
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- flax
- konjaku flour
- liquid
- nutrient medium
- degumming
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01C—CHEMICAL OR BIOLOGICAL TREATMENT OF NATURAL FILAMENTARY OR FIBROUS MATERIAL TO OBTAIN FILAMENTS OR FIBRES FOR SPINNING; CARBONISING RAGS TO RECOVER ANIMAL FIBRES
- D01C1/00—Treatment of vegetable material
- D01C1/04—Bacteriological retting
Abstract
The invention discloses a flax degumming liquid and a preparation method thereof, and relates to a plant fiber degumming liquid and a preparation method thereof, which solve the problems that in a current flax retting degumming method, added microorganisms contaminate a flax retting liquid and the environment, and an added pure enzyme preparation is greatly increased in cost and liable to inactivate. The flax degumming liquid is prepared by fermenting in a konjaku flour fluid nutrient medium for inoculating bacillus licheniformis HDYM-03, and centrifuging to get liquid supernatant. The flax degumming liquid is prepared by the following steps: inoculating the bacillus licheniformis HDYM-03 into the konjaku flour fluid nutrient medium to culture; and then, centrifuging to get the liquid supernatant, thereby obtaining the flax degumming liquid. The flax degumming liquid is short in degumming time, complete in degumming, low in residual gum content, high in cellulose yield and high in strength. The preparation method for the flax degumming liquid is simple and low in cost.
Description
Technical field
The present invention relates to a kind of string degumming baths and preparation method thereof.
Background technology
Flax (Linum usitatissimum) is annual herb plant, belongs to flax family (Linaceae) linum industrial crops, extensively plants in Europe and Asia.China is the second largest flax producing region in the world except Russia, and all there is plantation on the ground such as Xinjiang, Hunan and Heilungkiang.Linen fibre is the natural plant fibre that the mankind use the earliest, can trace back to the ancient Egypt's period before several thousand.The distinguishing features such as flax is pure natural fiber, has the effects such as moisture absorption is ventilative, antibiotic health care, harmless, at piece market, supply falls short of demand.
Namely the purification process of linen fibre removes glial component in flax straw, obtains the process of pure fiber, is called and comes unstuck.Warm water retting is the most widely used degumming tech of linen fibre manufacturing enterprise of China, is immersed in the water by flax straw, relies on naturally occurring microorganism in former stem and water to produce the outer degummase of born of the same parents, is hydrolyzed the glial component in former stem.In warm water retting process, naturally occurring micro organism quantity is few, degummase is active not high.Therefore, the retted fibre cycle is long and come unstuck not thorough, is the major drawbacks of warm water retting technique.
Adding bacterium retted fibre is add in retting liquid by having the microbial strains producing degummase ability, improves degummase vigor.But the existence of degumming bacterium and its metabolite can pollute retting liquid, bring the hidden danger of environmental pollution.
Enzyme process retted fibre adds pure enzyme preparation in retted fibre process, while improving efficiency of coming unstuck, can not bring environmental pollution.But the expensive and easy in inactivation of pure enzyme, adds production cost and does not reach again desirable degumming effect.
Summary of the invention
The object of the invention is to solve the microbial contamination retting liquid, the contaminated environment that add in method that current retted fibre comes unstuck; And add pure enzyme preparation cost and significantly rise and the problem of easy inactivation, and a kind of flax degumming liquid provided and preparation method thereof.
Flax degumming liquid by inoculating the konjaku flour solution culture fermentation of bacillus licheniformis (Bacillus licheniformis) HDYM-03, centrifuging and taking supernatant is prepared from; Wherein, konjaku flour fluid nutrient medium is made up of konjaku flour, yeast extract and distilled water, and konjaku flour fluid nutrient medium pH value is 6.0 ~ 7.0.
Above-mentioned flax degumming liquid is prepared according to the following steps:
Bacillus licheniformis (Bacillus licheniformis) HDYM-03 is inoculated in konjaku flour fluid nutrient medium, at 37 DEG C, shaken cultivation 48h under the condition of 140r/min, then centrifugal, get supernatant and namely obtain flax degumming liquid;
Wherein, konjaku flour fluid nutrient medium is made up of konjaku flour, yeast extract and distilled water, and konjaku flour fluid nutrient medium pH value is 6.0 ~ 7.0.
Find in research in the past that bacillus licheniformis (Bacillus licheniformis) HDYM-03 has very strong pectase and Mannanase Activity, and do not produce cellulase.But, in the zymotic fluid of bacillus licheniformis (Bacillus licheniformis) HDYM-03, do not detect that there is xylanase activity.And the present invention changes the metabolic pathway of bacillus licheniformis (Bacillus licheniformis) HDYM-03 by the mode changing culture medium and condition of culture, and then make it while generation pectase and mannase, produce zytase, and do not produce cellulase.
Flax degumming liquid ingredient of the present invention is few, only has konjaku flour and yeast extract in original culture medium, and along with fermentation carry out konjaku flour and yeast extract is constantly consumed, concentration further reduces; In addition through centrifugal treating, bacillus licheniformis (Bacillus licheniformis) HDYM-03 is also removed; Therefore retting liquid almost do not polluted and affect.
Containing pectase, mannase and zytase in flax degumming liquid of the present invention, and not cellulase; In flax degumming liquid, enzyme activity is high, and enzyme is lived stable; Glial component complicated in flax straw can be removed, have usually time short, come unstuck thoroughly, residual gum content is low, the advantage that cellulose yield is high, intensity is high.
The preparation method of flax degumming liquid of the present invention is simple, with low cost.Bacillus licheniformis (Bacillus licheniformis) continues to recycle and can reduce production cost after HDYM-03 is centrifugal.
Detailed description of the invention
Technical solution of the present invention is not limited to following cited detailed description of the invention, also comprises any combination between each detailed description of the invention.
Detailed description of the invention one: present embodiment flax degumming liquid by inoculating the konjaku flour solution culture fermentation of bacillus licheniformis (Bacillus licheniformis) HDYM-03, centrifuging and taking supernatant is prepared from; Wherein, konjaku flour fluid nutrient medium is made up of konjaku flour, yeast extract and distilled water, and konjaku flour fluid nutrient medium pH value is 6.0 ~ 7.0.
Present embodiment is bacillus licheniformis (Bacillus licheniformis) HDYM-03 for the preparation of the bacterial strain of flax degumming liquid, openly in " research of the separation of mannase producing strains, qualification and condition of enzyme production " and " HDYM03 produces the seed selection of mannase high productive mutant and the Primary Study of thick enzymatic property thereof " to record, be first preserved in Heilongjiang University, Universities of Heilongjiang Province key lab.
Detailed description of the invention two: the difference of present embodiment and detailed description of the invention one is: konjaku flour fluid nutrient medium is made up of 20g konjaku flour, 5g yeast extract and 1000mL distilled water in proportion, konjaku flour fluid nutrient medium pH value is 6.5.Other is identical with embodiment one.
Detailed description of the invention three: present embodiment flax degumming liquid is prepared according to the following steps:
Bacillus licheniformis (Bacillus licheniformis) HDYM-03 is inoculated in konjaku flour fluid nutrient medium, at 37 DEG C, shaken cultivation 48h under the condition of 140r/min, then centrifugal, get supernatant and namely obtain flax degumming liquid;
Wherein, konjaku flour fluid nutrient medium is made up of konjaku flour, yeast extract and distilled water, and konjaku flour fluid nutrient medium pH value is 6.0 ~ 7.0.
Detailed description of the invention four: the difference of present embodiment and detailed description of the invention three is: centrifugal rotational speed is 5000r/min, centrifugation time is 10min.Other step and parameter identical with embodiment three.
Detailed description of the invention five: the difference of present embodiment and detailed description of the invention three is: konjaku flour fluid nutrient medium is made up of 20g konjaku flour, 5g yeast extract and 1000mL distilled water in proportion, konjaku flour fluid nutrient medium pH value is 6.5.Other step and parameter identical with embodiment three.
Embodiment 1
Bacillus licheniformis (Bacillus licheniformis) HDYM-03 is inoculated in konjaku flour fluid nutrient medium, at 37 DEG C, shaken cultivation 48h under the condition of 140r/min, then centrifugal, centrifugal rotational speed is 5000r/min, centrifugation time is 10min, gets supernatant and namely obtains flax degumming liquid;
Wherein, konjaku flour fluid nutrient medium is made up of 20g konjaku flour, 5g yeast extract and 1000mL distilled water in proportion, and konjaku flour fluid nutrient medium pH value is 6.5.
The cell concentration of bacillus licheniformis (Bacillus licheniformis) HDYM-03 seed liquor is 10
8individual/mL, inoculum concentration is 2%.
In the present embodiment flax degumming liquid, the concentration of pectase is 500 ~ 700U/mL, and mannosan enzyme concentration is 2000 ~ 4000U/mL, zytase concentration is 100 ~ 300U/mL, cellulase concentration is < 5U/mL.
The flax degumming liquid prepared of the present embodiment is 20 ~ 40 DEG C in temperature, under the condition of pH value 4 ~ 6, preserves 60h pectase, mannase and Xylanase activity and all remain on more than 70% of initial enzyme activity.
The flax degumming liquid prepared by the present embodiment mixes with running water equal-volume, obtains flax degumming mixed solution.Flax straw joins room temperature in flax degumming mixed solution and to come unstuck 72h; Wherein, the ratio of flax straw and flax degumming mixed solution is 1kg:20L.
Contrast test
One, flax straw is soaked 1h in running water, remove surperficial soluble impurity and dust, earth.Discard running water, flax straw is divided into five parts, every part of 250g.Five parts of flax straws are laid in constant temperature (room temperature) water-bath, carry out retted fibre test with flax degumming mixed liquor, running water, bacillus licheniformis HDYM-03, commodity pectase and commodity mannase respectively.
Two, in the 1st water-bath, add 5000mL flax degumming mixed liquor, to be mixed with 2500mL running water by 2500mL flax degumming liquid and obtain.In 2nd water-bath, add 5000mL running water.In 3rd water-bath, add the running water bacteria suspension of 5000mL bacillus licheniformis HDYM-03, cell concentration is 10
6individual/mL.In 4th water-bath, add the polymer solution in water of 5000mL commodity pectase, pectinase activity 300U/mL.In 5th water-bath, add the polymer solution in water of 5000mL commodity mannase, mannosan enzyme activity 1500U/mL.
Three, running water retted fibre test period is 96h, and all the other four groups of retted fibre test periods are 72h.
Four, after retted fibre terminates, take out flax respectively and dry acquisition scapus in ventilation, then by machine scutching upper after scapus health 48h, obtain linen fibre.
Measure linen fibre intensity, fiber yield and residual gum content, result is as shown in table 1.
Table 1 linen fibre intensity, fiber yield and residual gum content
| Sequence number | Usually time (h) | Fibre strength (N) | Fiber yield (%) | Residual gum content (%) | |
| 1 | Flax degumming mixed liquor | 72 | 185.6±12.3 | 85.6±5.3 | 2.1±0.1 |
| 2 | Running water | 96 | 162.6±10.4 | 70.2±2.9 | 3.6±0.2 |
| 3 | Bacillus licheniformis HDYM-03 | 72 | 172.9±10.8 | 83.2±3.4 | 2.3±0.1 |
| 4 | Commodity pectase | 72 | 184.3±8.6 | 85.4±6.3 | 2.4±0.2 |
| 5 | Commodity mannase | 72 | 182.3±7.2 | 82.4±4.6 | 2.6±0.2 |
Flax degumming liquid of the present invention is used for flax straw and comes unstuck, have usually time short, come unstuck thoroughly, residual gum content is low, the advantage that cellulose yield is high, fibre strength is high.
In flax degumming liquid of the present invention (i.e. flax degumming mixed liquor), component of enzyme system is various, and can remove the colloid complex component in flax straw, therefore residual gum content is lower comprehensively.Owing to not containing microorganism in flax degumming liquid of the present invention, so avoid the problem of microbial contamination retting liquid, contaminated environment.Use the cost of flax degumming liquid of the present invention to be only and use 20% ~ 30% of pure enzyme preparation, and flax degumming better effects if.
Claims (5)
1. a flax degumming liquid, it is characterized in that flax degumming liquid by inoculating the konjaku flour solution culture fermentation of bacillus licheniformis (Bacillus licheniformis) HDYM-03, centrifuging and taking supernatant is prepared from; Wherein, konjaku flour fluid nutrient medium is made up of konjaku flour, yeast extract and distilled water, and konjaku flour fluid nutrient medium pH value is 6.0 ~ 7.0.
2. a kind of flax degumming liquid according to claim 1, it is characterized in that konjaku flour fluid nutrient medium is made up of 20g konjaku flour, 5g yeast extract and 1000mL distilled water in proportion, konjaku flour fluid nutrient medium pH value is 6.5.
3. the preparation method of flax degumming liquid described in claim 1, is characterized in that flax degumming liquid is prepared according to the following steps:
Bacillus licheniformis (Bacillus licheniformis) HDYM-03 is inoculated in konjaku flour fluid nutrient medium, at 37 DEG C, shaken cultivation 48h under the condition of 140r/min, then centrifugal, get supernatant and namely obtain flax degumming liquid;
Wherein, konjaku flour fluid nutrient medium is made up of konjaku flour, yeast extract and distilled water, and konjaku flour fluid nutrient medium pH value is 6.0 ~ 7.0.
4. the preparation method of flax degumming liquid according to claim 3, is characterized in that centrifugal rotational speed is 5000r/min, centrifugation time is 10min.
5. the preparation method of flax degumming liquid according to claim 3, it is characterized in that konjaku flour fluid nutrient medium is made up of 20g konjaku flour, 5g yeast extract and 1000mL distilled water in proportion, konjaku flour fluid nutrient medium pH value is 6.5.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1266096A (en) * | 1999-03-05 | 2000-09-13 | 中国科学院微生物研究所 | Method for producing alkaline 'beta'-mannase |
| CN102888372A (en) * | 2012-09-17 | 2013-01-23 | 贾平 | Bacillus and method for preparing textile fiber using same |
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2015
- 2015-05-26 CN CN201510271462.1A patent/CN104928763B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1266096A (en) * | 1999-03-05 | 2000-09-13 | 中国科学院微生物研究所 | Method for producing alkaline 'beta'-mannase |
| CN102888372A (en) * | 2012-09-17 | 2013-01-23 | 贾平 | Bacillus and method for preparing textile fiber using same |
Non-Patent Citations (3)
| Title |
|---|
| 平文祥等: "沤麻系统中—株甘露聚糖酶产生菌产酶条件的优化", 《黑龙江大学自然科学学报》 * |
| 赵丹: "甘露聚糖酶产生菌的分离、鉴定及产酶条件的研究", 《中国优秀博硕士学位论文全文数据库（硕士） 工程科技Ⅰ辑》 * |
| 黄小龙: "南方亚麻微生物脱胶技术及其机理研究", 《中国优秀博硕士学位论文全文数据库（硕士） 工程科技Ⅰ辑》 * |
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