CN104926935A - Efficient extraction method for extracting collagen from pigskin - Google Patents
Efficient extraction method for extracting collagen from pigskin Download PDFInfo
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- CN104926935A CN104926935A CN201410109101.2A CN201410109101A CN104926935A CN 104926935 A CN104926935 A CN 104926935A CN 201410109101 A CN201410109101 A CN 201410109101A CN 104926935 A CN104926935 A CN 104926935A
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Abstract
An efficient extraction method for extracting collagen from pigskin comprises the following steps: 1, removing impurities from fresh pigskin and then cleaning the fresh pigskin; 2, degreasing, to be more specific, putting the pigskin into a beaker container, adding 30-50 DEG C warm water with the weight of 4-5 times the weight of the pigskin, then adding a degreasing liquid of half volume of the water, stirring for 10-30 minutes, filtering supernatant liquid, repeating the degreasing once according to the above process, and finally rinsing with the 30-50 DEG C warm water; 3 salt extraction, to be more specific, taking the degreased pigskin, adding isotonic saline with the same amount of the pigskin to put into a water bath pot with constant temperature, keeping at 60 DEG C for 2-4 hours, then filtering and retaining the filtrate; 4, acid extraction, to be more specific, using an acetic acid buffer with the same amount of the pigskin for processing for 6-9 hours under 50-70 DEG C water bath conditions with the method same to salt extraction, then filtering and retaining the filtrate; 5, chopping, to be more specific, putting extraction residue into a chopping and mixing machine for chopping into 1-2mm, adding the filtrate while chopping; 6, drying; and 7, crushing and packaging. Through effective combining of the degreasing and acid method, the extraction process is improved, and the efficiency of collagen extraction is improved.
Description
(1) technical field
The present invention relates to a kind of extracting method of biological polymer substance, particularly a kind of highly effective extraction method extracting collagen protein from pigskin.
(2) background technology
Collagen protein is a kind of macromolecular glycoprotein, is mainly present in extracellular matrix, is the main composition of animal connective tissue, is distributed widely in the tissues such as animal skin, bone, blood vessel.Its structure is triple helix shape, and be made up of 3 polypeptide chains (α chain), every bar polypeptide chain is left hand helix structure, and three chains are wound around combination mutually, forms right-handed superhelix structure.The collagen types confirmed in land_atmosphere coupling model body at present has 27 kinds, also finds the existence of at least 2 kinds of collagen proteins in the muscle and skin histology of fish.In the many collagen types found, I-type collagen is the fibrillar collagen albumen that content enriches the most, accounts for more than 90% of whole collagen protein total amount.Collagen protein has multiple biomechanical property, for animal body and internal organs play a part support, protection and formed boundary every.In addition, it also has impact for the transport of cell signalling, cytokine, somatomedin etc.Because of its distinctive character and function, collagen protein has been widely used in comprising multiple different field such as makeup, food, biomedical material, organizational project.At present, the extraction of collagen is all generally for raw material with easy-to-handle beef tendon, mouse tail tendon etc., and the source of these raw materials is very limited, and extraction difficulty is large, cost is high, the cycle is long, simultaneously concerning beef tendon collagen, also there is the danger of mad cow disease, therefore, with other biological tissue for raw material extracts highly purified collagen, become a very important thinking.
(3) summary of the invention
Object of the present invention provides a kind of highly effective extraction method extracting collagen protein from pigskin, and degreasing and acid system combine and to extract Collagen Protein From Pig Skin by the method, effectively can improve extraction process, improves extraction efficiency.
As above conceive, technical scheme of the present invention is: a kind of highly effective extraction method extracting collagen protein from pigskin, is characterized in that: complete according to the following step:
1. clean after fresh porcine skin being removed impurity;
2. degreasing: be first placed in beaker container by pigskin, adds the warm water of pigskin weight 4-5 times 30-50 DEG C, and then adds the degreasing fluid of volume of water half, abundant stirring is after 10-30 minute, elimination supernatant liquid, repeats degreasing once as stated above again, finally uses 30-50 DEG C of warm water rinse;
3. salt is carried: after extracting degreasing, pigskin adds the isotonic saline solution of equivalent in thermostat water bath, at 60 DEG C, keep 2-4 hour, needs abundant stirring during constant temperature, makes pigskin thermally equivalent, then filters, and filtrate is retained, and prepares next step acid and carries;
4. acid is carried: under 50-70 DEG C of water bath condition, process 6-9 hour with the acetate buffer solution with pigskin equivalent, method and salt are carried identical, then filter, and filtrate is retained;
5. cut and mix: step extraction residuum is 4. put into choppingmachine and cut and mix to 1-2mm
2, Zhan Ban limit, limit adds filtrate;
6. dry;
7. pulverize, pack.
Above-mentioned degreasing fluid is by Na
2cO
3formulated, its concentration is 8% ~ 10%.
The pH=3.5 of above-mentioned acetate buffer solution.
The rotation speed requirements of above-mentioned steps 5. choppingmachine is more than 1500 revs/min, and choppingmachine Nei Wendu≤20 DEG C.
The present invention, by effective combination of degreasing, acid system technology, improves extraction process, improves the extraction efficiency of collagen protein.
1, skimming processes degreasing time of the present invention and stirring are fully, and there will not be the remaining phenomenon having grease in degreasing after product surface, therefore degreasing effect is obvious, for next step salt provides favourable condition.
2, repeatedly water to be changed in the whole operating process of the present invention, because the change of water temperature all exists very large impact to the purity of the product extracted and color, therefore process put forward by skimming processes in the present invention, salt, the acid water temperature carried in process keeps water temperature consistent, thus decreases and extract the open-assembly time of intermediate product under differing temps environment.
3, the most suitable by the amount of water in technical process, if water content is too low, then affect abundant extraction and the output of collagen protein; If too high, then do not have the effect of bonding and emulsification in use afterwards.Therefore, the addition appropriateness of water of the present invention.
(4) embodiment:
Below in conjunction with embodiment, the present invention will be further described:
From pigskin, extract a highly effective extraction method for collagen protein, complete according to the following step:
1, select materials: choose 200g fresh porcine skin as raw material skin.
2, repair: by fresh porcine skin decontamination, scrape off hair root, then remove subcutaneous lipids with cutter.Being cut into strip by picking the pigskin after fat, with 35 DEG C of warm water, pigskin strips being cleaned twice.
3, degreasing: first prepare degreasing fluid (by Na
2cO
3be mixed with the degreasing fluid that concentration is 8% ~ 10%).During degreasing, first pigskin strips is placed in beaker container, adds the warm water of pigskin weight 4-5 times 40 DEG C, and then add the degreasing fluid of volume of water half, fully stir after 20 minutes, elimination supernatant liquid, repeat degreasing more once as stated above, finally use 40 DEG C of warm water rinse twice.
4, salt is carried: use thermostat water bath control temperature.In the beaker putting pigskin strips, add the isotonic saline solution of equivalent, be placed in thermostat water bath, keep 3 hours at 60 DEG C.Ceaselessly to stir during constant temperature, be convenient to pigskin thermally equivalent.Filter, filtrate is retained, prepares next step acid and carry.
5, acid is carried: under 60 DEG C of water bath condition, process 7 hours with the acetate buffer solution (pH3.5) with pigskin strips equivalent, method and salt are carried identical, then filter, and filtrate is retained.Residuum after salt is carried and acid is carried is substantially without fur smell and other peculiar smell of pig itself, and its composition is mainly collagen protein and collagen protein macromole.
6, cut and mix: extraction residuum, more than 1500 revs/min, is put into choppingmachine and cut and mix to 1-2mm by choppingmachine rotation speed requirements
2be advisable, Zhan Ban limit, limit adds filtrate.During this period notably, controlling built-in temperature must not more than 20 DEG C.
7, dry: will the vacuum-drying at 28 DEG C of warm air dryings or 60 DEG C of the product after mixing be cut, vacuum tightness 0.08.
8, pulverize, pack: during pulverizing, notice that temperature can not be too high; Packaging should select PE packaging bags, and tying is tight, guards against damp, is stored in 4 DEG C of refrigerators.Obtain collagen protein.
Claims (4)
1. from pigskin, extract a highly effective extraction method for collagen protein, it is characterized in that: complete according to the following step:
1. clean after fresh porcine skin being removed impurity;
2. degreasing: be first placed in beaker container by pigskin, adds the warm water of pigskin weight 4-5 times 30-50 DEG C, and then adds the degreasing fluid of volume of water half, abundant stirring is after 10-30 minute, elimination supernatant liquid, repeats degreasing once as stated above again, finally uses 30-50 DEG C of warm water rinse;
3. salt is carried: after extracting degreasing, pigskin adds the isotonic saline solution of equivalent in thermostat water bath, at 60 DEG C, keep 2-4 hour, needs abundant stirring during constant temperature, makes pigskin thermally equivalent, then filters, and filtrate is retained, and prepares next step acid and carries;
4. acid is carried: under 50-70 DEG C of water bath condition, process 6-9 hour with the acetate buffer solution with pigskin equivalent, method and salt are carried identical, then filter, and filtrate is retained;
5. cut and mix: step extraction residuum is 4. put into choppingmachine and cut and mix to 1-2mm
2, Zhan Ban limit, limit adds filtrate;
6. dry;
7. pulverize, pack.
2. the highly effective extraction method extracting collagen protein from pigskin according to claim 1, is characterized in that: above-mentioned degreasing fluid is by Na
2cO
3formulated, its concentration is 8% ~ 10%.
3. the highly effective extraction method extracting collagen protein from pigskin according to claim 1, is characterized in that: the pH=3.5 of above-mentioned acetate buffer solution.
4. the highly effective extraction method extracting collagen protein from pigskin according to claim 1, is characterized in that: the rotation speed requirements of above-mentioned steps 5. choppingmachine is more than 1500 revs/min, and choppingmachine Nei Wendu≤20 DEG C.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410109101.2A CN104926935A (en) | 2014-03-21 | 2014-03-21 | Efficient extraction method for extracting collagen from pigskin |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410109101.2A CN104926935A (en) | 2014-03-21 | 2014-03-21 | Efficient extraction method for extracting collagen from pigskin |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105969830A (en) * | 2016-01-26 | 2016-09-28 | 成都市科乐生物技术有限公司 | Method for extracting active collagen peptide from pigskin |
| CN106361670A (en) * | 2016-09-24 | 2017-02-01 | 防城港市港口区思达电子科技有限公司 | Mask cream prepared from trotters |
| CN108186504A (en) * | 2018-03-26 | 2018-06-22 | 武汉轻工大学 | A kind of pigskin collagen facial mask and preparation method thereof |
| CN109810187A (en) * | 2017-11-20 | 2019-05-28 | 中国科学院大连化学物理研究所 | A kind of extracting method and application of animal tendon tendon collagen |
| CN112715744A (en) * | 2020-12-27 | 2021-04-30 | 西南大学 | Green preparation method of functional protein powder and product |
| CN114632183A (en) * | 2022-03-30 | 2022-06-17 | 温州医科大学 | Wound protein sponge, preparation method thereof and application of wound protein sponge in preparation of medicines for reducing scar formation in skin repair |
-
2014
- 2014-03-21 CN CN201410109101.2A patent/CN104926935A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105969830A (en) * | 2016-01-26 | 2016-09-28 | 成都市科乐生物技术有限公司 | Method for extracting active collagen peptide from pigskin |
| CN106361670A (en) * | 2016-09-24 | 2017-02-01 | 防城港市港口区思达电子科技有限公司 | Mask cream prepared from trotters |
| CN109810187A (en) * | 2017-11-20 | 2019-05-28 | 中国科学院大连化学物理研究所 | A kind of extracting method and application of animal tendon tendon collagen |
| CN108186504A (en) * | 2018-03-26 | 2018-06-22 | 武汉轻工大学 | A kind of pigskin collagen facial mask and preparation method thereof |
| CN112715744A (en) * | 2020-12-27 | 2021-04-30 | 西南大学 | Green preparation method of functional protein powder and product |
| CN114632183A (en) * | 2022-03-30 | 2022-06-17 | 温州医科大学 | Wound protein sponge, preparation method thereof and application of wound protein sponge in preparation of medicines for reducing scar formation in skin repair |
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Application publication date: 20150923 |
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