CN104922277B - Schisandra chinens P.E is in the application for preparing rescue paraquat poisoning drug - Google Patents
Schisandra chinens P.E is in the application for preparing rescue paraquat poisoning drug Download PDFInfo
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Abstract
The invention discloses Schisandra chinens P.E in the application for preparing rescue paraquat poisoning drug, experiment proves：Schisandra chinens P.E has good anti-oxidant; lung safeguard function, leads to paraquat injury of lungs to have significant protective effect, and the mechanism of effect may be formed with reducing oxygen radical; mitigate oxidative stress and inflammatory reaction, TGF β 1, IL 6 is inhibited to raise related.
Description
Technical field
The present invention relates to Schisandra chinens P.E in the application for preparing rescue paraquat poisoning drug.
Background technology
Paraquat (1,1'- dimethyl -4,4'- bipyridinium dichlorides；Paraquat, PQ) it is that a kind of the organic of severe toxicity connects
Touch property herbicide, the death rate of acute paraquat poisoned is more than>50%[1], there is document[2]Pointing out that 30mg/kg paraquats just have can
Can be fatal, this is equivalent to the paraquat solution 7-8ml of the 20% of market sale, and lung tissue active absorption enters the paraquat of human body
Lead to acute lung injury and pulmonary interstitial fibrosis.Paraquat also influences heart, kidney, adrenal gland, central nervous system, liver, bone
The internal organs such as bone flesh and spleen even result in multiple organ dysfunction syndrome (Multiple Organ Disfunction
Syndrome, MODS)[3]。
The paraquat acute poisoning also antidote without special efficacy at present, the medicine used at present and may have treatment to imitate
The drug of fruit includes：1. immunosuppressor：Cyclophosphamide, glucocorticoid；2. Antioxidation Treatment：Reduced glutathione
(GSH), vitamin family, acetylcysteine (NAC), 2 inhibitor of quinone oxidoreductase, Yi Dalafeng, sodium salicylate (SA)；
3. Chinese medicine is treated：There are some researchs to find that curcumin, rheum officinale, Xue bijing etc. have acute paraquat poisoned injury of lungs certain treatment
Effect[3、4]。
Schisandra chinensis is magnoliaceae schisandra Schisandra chinensis (Turcz.) Baill. or Central China five tastes
The dry mature fruit of sub- Schisandra sphenanthera Rehd.et Wils..The former practises title " fructus schisandrae ", the latter
It practises and claims " kadsura longepedunculata ".It picks, dry or is dried after steaming when the fruit matures in the fall, remove carpopodium and impurity.Tang etc.《Xin Xiuben
Grass》" five tastes skin and flesh sweet acid works hard in core, there is saline taste " is carried, therefore has the name of Schisandra chinensis.Schisandra chinensis is divided into two kinds of south, north.Gu doctor
Book Cheng Ta Chinese magnoliavines, Chinese magnoliavine, meeting and, be listed in Sheng Nong's herbal classic top grade Chinese medicine earliest, can strengthening by means of tonics power, medical value is high,
The benefits of keeping fit and healthy.Effect：It astringes the lung, nourshing kidney is promoted the production of body fluid, and receives sweat, puckery essence.It controls the deficiency syndrome of the lung to breath with cough, thirst, spontaneous perspiration, steal
Sweat, internal lesion caused by overexertion is thin thin, emission, protracted dysentery and diarrhea.Modern Chinese medicine pharmacological research finds that Schisandra chinens P.E contains lignanoid, thick more
The compositions such as sugar, volatile oil and general flavone, and this some composition is proved to have good anti-oxidant, scavenging activated oxygen, increases
The effect of strong immunity.Schisanhenol is directed to oxidative stress injury of mitochondria and plays significant protective effect[5].The present invention
Emphasis using Schisandra chinens P.E as research object, investigate its be poisoned to mouse PQ Acute Lung Injury and pulmonary fibrosis and control
Treatment acts on.
Invention content
The purpose of the present invention is overcoming deficiency of the prior art, Schisandra chinens P.E is provided in rescue paraquat is prepared
The application of cytotoxic drug.
Second object of the present invention is to provide the preparation containing Schisandra chinens P.E and is preparing rescue paraquat poisoning drug
Application.
Technical scheme of the present invention is summarized as follows：
Schisandra chinens P.E is in the application for preparing rescue paraquat poisoning drug.
The Schisandra chinensis is fructus schisandrae (Schisandraceae) or kadsura longepedunculata (Kadsurajaponica).
Preparation containing Schisandra chinens P.E is in the application for preparing rescue paraquat poisoning drug.
The preparation is preferably tablet, capsule, granule or injection.
Experiment proves：Schisandra chinens P.E have it is good anti-oxidant, lung safeguard function leads to injury of lungs to paraquat
With significant protective effect, the mechanism of effect may be formed with reducing oxygen radical, mitigate oxidative stress and inflammatory reaction,
Inhibit TGF-β 1, IL-6 up-regulations related.
Description of the drawings
Fig. 1 is Schisandra chinens P.E to mouse survival quantity after large dosage of paraquat poisoning.
Fig. 2 Schisandra chinens P.Es averagely eat mouse after large dosage of paraquat poisoning weight.
Fig. 3 influences (100 times and 200 times of amplification) paraquat poisoning mouse lung tissue HE dyeing for Schisandra chinens P.E.
A:Blank control group B:Paraquat group (300mg/kg) C:Paraquat+vitamin C group D:Paraquat+Schisandra chinens P.E group
(200mg/kg)E:Paraquat+Schisandra chinens P.E group (400mg/kg) F:Paraquat+Schisandra chinens P.E group (800mg/kg).
Fig. 4 Schisandra chinens P.Es influence (200 times of amplification) paraquat poisoning mouse lung tissue Masson dyeing.A:Blank
Control group B:Paraquat group (300mg/kg) C:Paraquat+vitamin C group D:Paraquat+Schisandra chinens P.E group (200mg/kg)
E:Paraquat+Schisandra chinens P.E group (400mg/kg) F:Paraquat+Schisandra chinens P.E group (800mg/kg)
Influence of Fig. 5 Schisandra chinens P.Es to PQ poisoning mice serum GSH and lung tissue MDA levels
Influence of Fig. 6 Schisandra chinens P.Es to PQ poisoning mice lung tissues TGF-β 1 and IL-6 levels
Specific embodiment
Schisandra chinens P.E used in the present invention is extracted using ethanol reflux extraction.
Following public preparation process is to enable those skilled in the art to more fully understand the present invention, but not right
The present invention imposes any restrictions.
Embodiment 1
The extraction of Schisandra chinens P.E：
The ethanol water for taking fructus schisandrae raw material 500g that the volumetric concentration of 8 times of material qualities is added to be 95%, is heated to reflux
Extraction 2 times, 3h/ times, filtering, merging filtrate is recovered under reduced pressure ethyl alcohol and is concentrated into medicinal extract, 48h is lyophilized, obtains red-brown powder, calculates
Yield is 23.5%, and total lignan content is 5.5% in powder.
It is extracted using other parameters, in the range of yield is 10%-40%, total lignan content is 5- in powder
20% Schisandra chinens P.E may be used to the present invention.
Kadsura longepedunculata extracts as stated above, and in the range of yield is 10%-40%, total lignan content is 5- in powder
20% Schisandra chinens P.E may be used to the present invention.
Embodiment 2
Pharmacological evaluation：
The research that Schisandra chinens P.E acts on the rescue of large dosage of paraquat poisoning：
1 materials and methods
1.1 experimental animals and material
Schisandra chinens P.E (embodiment 1 obtains) is configured before administration with physiological saline；
Vitamin C injection：Tianjin Kingyork Pharmaceutical Co., Ltd. (Chinese medicines quasi-word H12020392)；
4 SPF grades of week old male Kunming small white mouse 72 (Beijing Vital River Experimental Animals Technology Co., Ltd.), weight
(21±2.4)g；
20% paraquat liquid:Shanghai Shanghai connection biology medicine company (Xiayi) Co., Ltd (product standard GB 19308-
2003)；
GSH detection kits:Bioengineering Research Institute is built up in Nanjing；
MDA detection kits：The green skies；
Mouse TGF-β 1 and IL-6 (ELISA) kit：R＆D companies of the U.S.；
IL-17 antibody:Article No. ab79056, abcam companies of the U.S..
1.2 method
1.2.1 the foundation experiment of acute PQ poisonings model of lung injury is divided into：
Blank control group (Con)；
Paraquat group (PQ)；
PQ+SCEs (200mg/kg, 400mg/kg, 800mg/kg) group；(SCEs is the abbreviation of Schisandra chinens P.E)
PQ+vitamin C (100mg/kg) group.
Every group 12.
The foundation of model：The disposable gavage PQ liquid of mouse, dosage 300mg/kg.
Medication：24th hour gavage SCEs of the PQ+SCEs groups after model foundation is primary, and PQ+vitamin C groups exist
The 24th hour intraperitoneal injection vitamin C after model foundation is primary, and PQ groups and Con groups are replaced with isodose physiological saline,
Then daily every group of mouse is administered once as stated above, continues 21 days.
1.2.2 serum prepares mouse orbit and takes blood, room temperature blood natural coagulation 20 minutes, centrifugation 20 minutes (3000 turns/
Point).Collect supernatant, -80 DEG C of preservations.
1.2.3 after lung homogenate prepares Rat Right lung homogenate, 1600g is centrifuged 10 minutes, and supernatant is taken to be used for subsequently
It measures.
1.2.4 after animal lungs are won in mouse lung tissue HE dyeing and Masson dyeing, after observing general condition, with 10%
Neutral formalin is fixed, and is embedded, is sliced.HE is dyed：Carry out conventional haematoxylin and eosin stains 10 minutes；Masson is dyed：It is beautiful
Spring, red acid fuchsin liquid dyed 10 minutes, was developed a film quarter with 2% glacial acetic acid aqueous solution, and 1% phosphomolybdic acid aqueous solution breaks up 5 minutes,
It is directly contaminated 5 minutes with aniline blue without washing, the pathomorphism of light Microscopic observation lung tissue.
1.2.5 serum GSH Activity determinations and lung tissue MDA detections take serum and lung homogenate, in strict accordance with kit
Defined method carries out absorbance measurement, by formula scales into GSH and MDA concentration.
1.2.6 lung tissue TGF-β 1 and IL-6 detections take lung homogenate, ELISA kit detection TGF-β 1 and IL-6,
Specific method is carried out according to specification.
1.2.7 the lung tissue of embedding is carried out frozen section (6 μ by the expression of immuno-fluorescence assay lung tissue IL-17
M), paraformaldehyde is fixed, is dehydrated；With 4 DEG C of overnight incubations of IL-17 antibody, secondary antibody is incubated, is developed a film, fluorescence microscope is seen after mounting
It examines.
All data of 1.3 statistical methods are analyzed using 21.0 softwares of SPSS, data withIt represents, between group
Compare using one-way analysis of variance, inspection level α=0.05, P<0.05 is statistically significant for difference.
2 results
2.1 mouse survival rates and food-intake observation：There is death in first day in contamination in paraquat group, is only deposited at the 8th day
1 animal living；PQ+SCEs each group survival rates are significantly increased, and especially high concentration group (800mg/kg) animal survives 5, and
Food-intake is significantly higher than paraquat group, illustrates that Schisandra chinens P.E can rescue high concentration acute paraquat poisoned (Fig. 1,2).
2.2 om observation HE is dyed：PQ groups mouse lung tissue loses normal configuration compared with Con groups, and alveolar wall collapses, lung
Bubble intracavitary has the performances such as exudate, telangiectasis, hyperemia；PQ+SCEs (200,400,800mg/kg) organizes mouse injury of lungs
Pathological change is substantially reduced compared with PQ groups, and with the increase of SCEs concentration, and pathological change is gradually reduced；PQ+SCEs
(800mg/kg) group mouse alveolar wall collapses considerably less than PQ+vitamin C groups (Fig. 3).Masson is dyed：PQ group mouse lung groups
It is broadening to knit under light microscopic performance alveolar spaces compared with Con groups, extracellular matrix also apparent hyperplasia has and largely contaminates green collagenous fibres
Hyperplasia deposits, and forms extensive fibrosis；And PQ+SCEs group mouse pulmonary fibrosis degree significantly mitigates compared with PQ groups；PQ+
SCEs (800mg/kg) group mouse lung tissue Collagen fiber depositions are less than PQ+vitamin C groups (Fig. 4).
2.2 SCEs to the influence PQ groups mice serum GSH contents of PQ poisoning mice serum GSH and lung tissue MDA contents with
Con groups are compared and substantially reduce (p<0.01), MDA contents significantly increase (p<0.001)；PQ+SCEs each groups serum GSH contents and PQ
Group, which is compared, significantly increases (p<0.05)；PQ+SCEs (800mg/kg) organizes mice serum GSH contents compared with PQ+vitamin C groups
It is apparent to increase (p<0.05) (Fig. 5 A).PQ+SCEs (400mg/kg, 800mg/kg) lung tissue MDA contents significantly reduce (p<
0.01), PQ+SCEs (200mg/kg) organizes mouse lung tissue MDA contents compared with PQ groups without significant change；PQ+SCEs(400mg/
Kg, 800mg/kg) group mouse lung tissue MDA contents significantly reduced compared with PQ+vitamin C groups (Fig. 5 B).Above result
The SCEs that can prove larger concentration can effectively mitigate mouse PQ poisoning body oxidative stress, reduce oxygen radical pair
The damage of body.
2.3 SCEs are to the influence PQ group mouse lung tissues TGF-β 1 of PQ poisoning mice lung tissues TGF-β 1 and IL-6 contents
And IL-6 Average expression levels dramatically increase (p compared with Con groups<0.01)；PQ+SCEs group mouse lung tissues TGF-β 1 (Fig. 6 A)
With IL-6 (Fig. 6 B) expression (p is significantly reduced compared with PQ groups<0.05)；
PQ+SCEs (800mg/kg) organizes mouse lung tissue IL-6 expressions compared with PQ+vitamin C groups without significance difference
Different (p>0.05).The above results show that SCEs can effectively reduce PQ poisoning mice lung tissues TGF-β 1 and IL-6 expression.
In conclusion SCEs has PQ induced mices injury of lungs significant protective effect, the mechanism of effect may be with
It reduces oxygen radical to be formed, mitigates oxidative stress and inflammatory reaction, inhibit TGF-β 1, the up-regulation of IL-6 related.
Tablet, capsule, granule or injection is made in Schisandra chinens P.E according to a conventional method.
Bibliography
[1]Gawarammana IB,Buckley NA.Medical management of paraquat ingestion
[J].Br J Clin Pharmacol,2011,72(5):745-757.
[2] Xu Lingjie, Wang Zhong paraquats cause acute lung injury Recent Advances in Mechanism [J] clinical emergency magazines, 2013,14
(4)：186-189.
[3] Dinis Oliveira R J, Duarte J A, S á nchez-Navarro A, et al.Paraquat
poisonings:Mechanisms of lung toxicity, clinical features and treatment [J]
.Crit Rev Toxicol, 2008,38 (1):13-71.
[4] it is miscellaneous (to write) eastwards paraquat poisoning Clinics and Practices " Mount Taishan common recognition " (2014) [J] Chinese industrial medicine for pipe
Will, 2014,27 (2)：117-119.
[5] Liu Wei armies blood perfusions 5 are treated severe paraquat poisoning observation of curative effect [J] Contemporary Chineses with bleaching earth and are cured
Medicine, 2011,18 (4):26-27.
[6] application [J] China emergency resuscitation of Wang Jinchun, Li Yan the brightness gastrointestinal tract purification treatments in paraquat poisoning with
Disaster medicine magazine, 2012,7 (3):269-270.
Claims (4)
1. Schisandra chinens P.E is in the application for preparing treatment paraquat Lung Injury drug.
2. application according to claim 1, it is characterized in that the Schisandra chinensis is fructus schisandrae or kadsura longepedunculata.
3. the preparation containing Schisandra chinens P.E is in the application for preparing treatment paraquat Lung Injury drug.
4. the preparation according to claim 3 containing Schisandra chinens P.E is preparing treatment paraquat Lung Injury drug
Using it is characterized in that the preparation is tablet, capsule, granule or injection.
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| CN110585180A (en) * | 2019-09-25 | 2019-12-20 | 中国热带农业科学院环境与植物保护研究所 | Specific antidote for treating paraquat acute poisoning |
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| CN104490892A (en) * | 2014-12-02 | 2015-04-08 | 江南大学 | Therapeutic method of methylene blue in liver injury protection at early paraquat poisoning stage |
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| 中西医结合救治百草枯中毒的临床探讨;董慧慧等;《浙江临床医学》;20140331;第16卷(第3期);第429页 * |
| 五味子木脂素的抗癫痫作用及神经保护研究;张国辉;《中国优秀硕士学位论文全文数据库 医药卫生科技辑》;20141015(第10期);第6页 * |
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