CN104909463A - Core-shell structured water purification bacterium capsule and preparation method thereof - Google Patents
Core-shell structured water purification bacterium capsule and preparation method thereof Download PDFInfo
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Abstract
The invention provides a core-shell structured capsule embedding a water purification bacterium agent and slowly releasing a carbon source. The capsule has a core-shell structure, wherein the capsule core is a composite slow release carbon source, and is a pre-molded homogeneous solid mixed carbon source block; and the capsule shell is an organic-inorganic composite porous material, the water purification bacterium agent is embedded in the porous material, and the capsule is prepared through embedding the core in the shell and carrying out solidification processing molding. The core-shell structured water purification capsule is simple to process, the capsule core has a long carbon source release cycle, the capsule shell has the characteristics of high strength, good water penetration and high bacterium agent water purification activity, and the capsule is dissolved with the complete release of the soluble carbon source, so the capsule has a macroscopic self-consumption indication characteristic, and the consumption condition of the capsule can be conveniently judged by naked eyes. The capsule has high application potential in targeted efficient purifying treatment of polluted water.
Description
Technical field
The present invention relates to the immobilization embedded field of microbial inoculum, mainly relate to a kind of water purification microbial inoculum that embeds and to ease up nucleocapsid structure capsule releasing carbon source and preparation method thereof.
Background technology
Microorganism plays the role that can not be substituted in polluted-water restoration of the ecosystem, plays an important role in reduction chemical oxygen demand of water body, ammonia nitrogen and total nitrogen etc.In polluted-water restoration of the ecosystem process, manually adding microbial inoculum can run off with water body flow or be deposited to the bottom, and cause effect to reduce, it is unfavorable efficiently to play the fixed point of microbial inoculum water purification performance.Being fixed of water purification microbial inoculum is processed, and is aided with the performance that suitable carbon source promotes its activity, then can evade the problem of loss and sedimentation, and can purifying water effect be improved, be beneficial to the efficient improvement of polluted-water fixed point.
Through finding the retrieval of prior art, the immobilization embedded research of microbial inoculum is more, common polymer wall material is as sodium alginate, polyvinyl alcohol, carrageenin, polyglutamic acid etc., and common auxiliary material is as gac, diatomite, zeolite powder, clay, expanded perlite powder and polyurethane foam etc.In the immobilization embedded application of common polymer wall material microbial inoculum, existing independent application, also there is Combination application, as sodium alginate-carrageenin combination, sodium alginate-PVOH combination, carrageenin-sodium alginate-polyvinyl alcohol combination etc.; In complete processing, some employing freeze forming methods, the fixed-type method of employing solid solution had, the method also having the first freezing after fixing of employing shaping; In product type, there are film-type, particulate state and have bulk etc., be the structure that inside and outside quality is consistent, have no nucleocapsid structure microbial inoculum embedding thing.
Summary of the invention
The invention discloses a kind of nucleocapsid structure water purification bacterium capsule and preparation method thereof.The core of this nucleocapsid structure capsule is mixing slow release carbon source, and shell is the Organic-inorganic composite porous material of load water purification bacterium.Carbon source is discharged into after in shell and is preferentially utilized by load microbial inoculum, and has the effect of stable capsule shell structure, and after carbon source approach exhaustion, capsule shell can be caved in deliquescing, until last corrosion is completely fallen.
Therefore nucleocapsid structure water purification capsule of the present invention is except possessing the efficient water purification activity of microbial inoculum immobilization and slow release carbon source support, also there is Expenditure Levels oneself indicative function, by reducing the observation of situation to the change of capsule macro geometry and storage, capsule consumption degree can be judged, be convenient to supplement in good time.Contrast with prior art and find, there is not been reported for the nucleocapsid structure water purification capsule characteristics that the present invention relates to, composite slow release carbon source formula and complete processing have no report, the capsule shell formula formed by agar, sodium alginate, diatomite and complete processing have no report, also have no report with the mentality of designing of capsule core carbon source regulation and control capsule shell loss.
The object of the invention is to invent a kind of purifying water micrcxDrganism immobilization preparation simultaneously embedding carbon source and microbial inoculum, in order to improve the load factor of carbon source, realize the controlled slow releasing of carbon source, improve immobilized microbial inoculum to the utilising efficiency rate of carbon source, guarantee that immobilized microbial inoculum contacts fully with extraneous water body, present invention employs the mentality of designing of nucleocapsid structure.Capsule core is the mixed carbon source through slowly-releasing process, and capsule shell is the Organic-inorganic composite porous material of embedding water purification microbial inoculum.The main component of mixed carbon source comprises: Trisodium Citrate, stearic acid, calcium acetate, sodium-acetate, calcium chloride, xanthan gum, sodium alginate and sucrose; The main component of capsule shell comprises: water purification microbial inoculum, agar, sodium alginate and diatomite.
On the one hand, the invention provides a kind of nucleocapsid structure capsule embedding slow release carbon source and water purification microbial inoculum, it is characterized in that: capsule is nucleocapsid double-layer structure, wherein, slow release carbon source is capsule core, and main component comprises: Trisodium Citrate, stearic acid, calcium acetate, sodium-acetate, calcium chloride, bamboo charcoal, calcium carbonate, xanthan gum, sodium alginate and sucrose etc.
Preferably, the Organic-inorganic composite porous material of embedding water purification microbial inoculum is capsule shell, and main component comprises: water purification microbial inoculum, agar, sodium alginate and diatomite, the total viable count 2.0 × 10 of water purification bacterium
7~ 2.0 × 10
9cFU/g weight in wet base capsule shell; Wherein, the Bacillus licheniformis DSM 13 that described water purification microbial inoculum is ATCC14580 for the preserving number subtilis BJ that is CGMCC No.2288 and preserving number is produced.
Preferably, described capsule core is the mixture of Trisodium Citrate, stearic acid, calcium acetate, sodium-acetate, calcium chloride, bamboo charcoal, calcium carbonate, xanthan gum, sodium alginate and sucrose, it can be arbitrary shape, as ball-type, square, column, pie, olive-type, star-like etc., maximum physical dimension can be 1.0cm ~ 50cm.Capsule shell is subtilis BJ and Bacillus licheniformis DSM 13 bacterium powder, agar, sodium alginate and diatomaceous mixture, and be coated on capsule core surface, thickness is 0.2cm ~ 10cm, microbial inoculum encapsulation rate 90 ~ 99%.Preferably, wherein the mass percentage content of Trisodium Citrate is 40 ~ 70%, stearic mass percentage content is 1 ~ 10%, the mass percentage content of calcium acetate is 1 ~ 20%, the mass percentage content of sodium-acetate is 1 ~ 10%, the mass percentage content of calcium chloride is 10 ~ 40%, the mass percentage content of bamboo charcoal is 0.1 ~ 20%, the mass percentage content of calcium carbonate is 0.1 ~ 30%, the mass percentage content of xanthan gum is 0.5 ~ 4%, the mass percentage content of sodium alginate be 0.5 ~ 4% and the mass percentage content of sucrose be 0.1 ~ 1%.Preferably: Trisodium Citrate 53%, stearic acid 6%, calcium acetate 10%, sodium-acetate 2%, calcium chloride 20%, bamboo charcoal 5%, calcium carbonate 0.1%, xanthan gum 2.4%, sodium alginate 1% and sucrose 0.5%.
On the other hand, the present invention relates to the preparation method of above-mentioned nucleocapsid structure water purification bacterium agent capsules, comprise the following steps:
The first step, configuration capsule core material, i.e. slow release carbon source mixture: by Trisodium Citrate, stearic acid, calcium acetate, sodium-acetate, calcium chloride, bamboo charcoal, calcium carbonate, xanthan gum, sodium alginate and sucrose mix in proportion, wherein the mass percentage content of Trisodium Citrate is 40 ~ 70%, stearic mass percentage content is 1 ~ 10%, the mass percentage content of calcium acetate is 1 ~ 20%, the mass percentage content of sodium-acetate is 1 ~ 10%, the mass percentage content of calcium chloride is 10 ~ 40%, the mass percentage content of bamboo charcoal is 0.1 ~ 20%, the mass percentage content of calcium carbonate is 0.1 ~ 30%, the mass percentage content of xanthan gum is 0.5 ~ 4%, the mass percentage content of sodium alginate be 0.5 ~ 4% and the mass percentage content of sucrose be 0.1 ~ 1%.Optimal proportions is: Trisodium Citrate 53%, stearic acid 6%, calcium acetate 10%, sodium-acetate 2%, calcium chloride 20%, bamboo charcoal 5%, calcium carbonate 0.1%, xanthan gum 2.4%, sodium alginate 1% and sucrose 0.5%;
Second step, capsule core makes shaping: mixed according to mass ratio=10:1 ~ 10:5 with water by the mixture of the first step, make dough or pasty material, and be processed into desired shape, then dries in 50 ~ 100 DEG C and obtains moulding capsules kernel.
3rd step, capsule shell material prepares: 0.5 ~ 4.0g agar, 0.5 ~ 5.0g sodium alginate and 20 ~ 50g diatomite are joined in 100mL water, is heated to dissolve fully.When temperature is reduced to 50 DEG C, add subtilis BJ bacterium powder 0.2 ~ 2.0g and Bacillus licheniformis DSM 13 bacterium powder 0.1 ~ 2.0g, and the acquisition outer shell material that stirs rapidly, for subsequent use at 45 DEG C.
4th step, prepared by nucleocapsid capsule: pour in the mould of definite shape by the outer shell material that the 3rd step obtains, and then the moulding capsules kernel that second step obtains is embedded outer shell material inner, kernel is submerged in outer shell material completely, cooling forming under room temperature.In left at room temperature, leave standstill process agar Yin Wendu and reduce and solidify, thus make capsule shaping.The combination of outer shell material formula agar, sodium alginate and diatomite has irreplaceability.As sodium alginate is replaced to common polyvinyl alcohol, then can not curing molding; As diatomite is replaced with bamboo charcoal, then capsule hardness declines to a great extent; As removed by sodium alginate, capsule hardness declines to a great extent; As removed by diatomite, then capsule hardness and water-permeable all decline to a great extent, and reduce more than 50% and can not recover after rehydration after capsule drying.
5th step, capsule shell solidification and capsule drying treatment: the capsule of the 4th step cooling forming is immersed 0.5 ~ 4h in 5 ~ 10% calcium chloride solutions, then take out oven dry at 20 ~ 40 DEG C and spend the night and can obtain the final nucleocapsid structure capsule of the present invention.
The degree that the present invention is all, not having special for indicating, all belonging to mass percentage content.
The present invention, by regulating the degree regulation and control carbon source release behavior of stearic acid, calcium chloride, xanthan gum and sodium alginate in capsule core, can obtain the serial slow release carbon source kernel of carbon source release characteristic significant difference.Such as improve the content of stearic acid, xanthan gum and sodium alginate, the release rate of Trisodium Citrate, calcium acetate, sodium-acetate and sucrose all reduces, otherwise release rate raises; Improve the content of calcium chloride, calcium acetate and calcium carbonate, citric acid release rate reduces, otherwise raises.Can regulate capsule shell stability additionally by regulation and control capsule core composition.As synchronously improved Trisodium Citrate, calcium carbonate, calcium chloride and calcium acetate content in proportion, capsule shell stability can be improved, extending capsule shell effect duration; Otherwise capsule shell stability lowers, effect duration shortens.In addition, by regulating the thickness of capsule core size and capsule shell, can realize the regulation and control of carbon source deenergized period, more large capsule shell is thicker for capsule core, and carbon source is longer for deenergized period; Otherwise capsule core is less, capsule shell is thinner, and carbon source is shorter for deenergized period.Finally, by using difform mould, the nucleocapsid structure capsule of the present invention of arbitrary shape can be produced.
Nucleocapsid structure capsule prepared by the present invention, needed for its kernel carbon source release 90%, duration is: 1 ~ 60 month; The required duration of capsule shell clear dissolution collapse is: 1 ~ 70 month; In capsule shell, viable count is 2.0 × 10
7~ 2.0 × 10
9cFU/g weight in wet base capsule shell, wherein subtilis BJ is 1.0 × 10
7~ 1.0 × 10
9cFU/g weight in wet base capsule shell, ground bacillus DSM13 is 1.0 × 10
7~ 1.0 × 10
9cFU/g weight in wet base capsule shell.
Beneficial effect
Preparation manipulation of the present invention is simple, gained nucleocapsid capsule is one-body molded, carbon source not easily occurs reveal, be applied to town and country polluted river water and can play excellent fixed point high-efficiency sewage regulation effect, in reduction chemical oxygen demand (COD), water body ammonia nitrogen and total nitrogen etc., performance is excellent.
Accompanying drawing explanation
Fig. 1 is nucleocapsid capsule structure schematic diagram of the present invention.
Fig. 2 is capsule appearance in the present invention's embodiment and sectional view.
Fig. 3 is capsule shell inner porosity figure (structure iron under electron microscope).
Embodiment
Elaborate to embodiments of the invention below, the present embodiment is implemented under premised on technical solution of the present invention, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
The nucleocapsid structure capsule relating to embedding slow release carbon source and water purification microbial inoculum of following examples comprises: capsule shell two portions of slow release carbon source capsule core and embedding water purification microbial inoculum.Wherein capsule core is made up of Trisodium Citrate, stearic acid, calcium acetate, sodium-acetate, calcium chloride, bamboo charcoal, calcium carbonate, xanthan gum, sodium alginate and sucrose, and capsule shell is made up of microbial inoculum, agar, sodium alginate and diatomite.Prefabricated profiled capsule core is embedded in the capsule shell solution of not yet curing molding, treats that shell curing molding can obtain nucleocapsid structure capsule of the present invention.
Microbial inoculum in following all examples of implementation is produced by subtilis and Bacillus licheniformis powder.Wherein, subtilis BJ and (subtilis BJ, Classification And Nomenclature: subtilis BJ, Classification system: Bacillus subtilis BJ, preserving number: CGMCC No.2288, depositary institution is: China General Microbiological culture presevation administrative center) authorize patent of invention, exercise question: for the microbial preparation and preparation method thereof of purifying aquatic product cultivating water body, application number 200810059104, middle told subtilis BJ is same bacterial strain; Bacillus licheniformis DSM 13 (Bacillus licheniformis DSM 13, Classification And Nomenclature: Bacillus licheniformis DSM 13, Classification system: Bacillus licheniformis DSM 13, preserving number: ATCC14580, depositary institution: American type culture collection (purchased from Southern Yangtze University's industrial microorganism resource and information center).
Embodiment 1
Step 1: by 40g Trisodium Citrate, 1g stearic acid, 1g calcium acetate, 1g sodium-acetate, 10g calcium chloride, 15.9g bamboo charcoal, 30.0g calcium carbonate, 0.5g xanthan gum, 0.5g sodium alginate and 0.1g sucrose mix.Then add 10g water to mix, be modulated into dough carbon source mixture.
Step 2: by dough carbon source mixture press-in die, make diameter 1.0cm, the column of high 1.0cm, then drying forming at 50 DEG C, obtains capsule core.
Step 3: 0.5g agar and 0.5g sodium alginate are scattered in 100mL water, then heating makes agar and sodium alginate fully dissolve, then add 20g diatomite to mix, to be cooledly obtain capsule shell material to adding bacterium powder 0.2g after 50 DEG C and mixing, then for subsequent use at 45 DEG C.
Step 4: mixing capsule outer shell material step 3 prepared pours diameter 1.4cm into, in the cylindrical mold of high 1.4cm.
Step 5: to be embedded into by step 2 gained capsule core in step 4 in capsule shell material, makes capsule core be positioned at mould central authorities and be flooded completely by capsule shell material.Then, under room temperature, cooled and solidified is shaping.
Step 6: taken out by the capsule of step 5 coagulation forming, immerses in the calcium chloride solution of 5% and solidifies 0.5h, then takes out to dry at 20 DEG C and spends the night, and namely obtains the nucleocapsid structure capsule embedding slow release carbon source.
This capsule is nucleocapsid structure, the maximum physical dimension of capsule core is 1.0cm, in kernel, each constituent mass percentage composition is Trisodium Citrate 40%, stearic acid 1.0%, calcium acetate 1.0%, sodium-acetate 1.0%, calcium chloride 10%, bamboo charcoal 15.9%, calcium carbonate 30.0%, xanthan gum 0.5%, sodium alginate 0.5%, sucrose 0.1%, and capsule shell thickness is 0.2cm; Kernel molding process, the mass ratio of material and water is 10:1, and bake out temperature is 50 DEG C; The each component concentration of shell is agar 0.5g in every 100mL water, sodium alginate 0.5g, diatomite 20.0g, bacterium powder 0.2g, and in shell, subtilis BJ and Bacillus licheniformis DSM 13 viable count are 1.0 × 10
7cFU/g weight in wet base capsule shell; Capsule shell solidifying agent to be mass percent concentration be 5.0% calcium chloride water, solidification duration is 0.5h, and microbial inoculum encapsulation rate is 90.0%, and cured glue capsule bake out temperature is 20 DEG C; The cycle that capsule core discharges 90% water-soluble carbon source is 1.0 months, and the cycle of release 85% embedding microbial inoculum is 1.0 months.
Embodiment 2
Step 1: by 70g Trisodium Citrate, 1g stearic acid, 1g calcium acetate, 1g sodium-acetate, 25.7g calcium chloride, 0.1g bamboo charcoal, 0.1g calcium carbonate, 0.5g xanthan gum, 0.5g sodium alginate and 0.1g sucrose mix.Then add 50g water to mix, be modulated into dough carbon source mixture.
Step 2: by dough carbon source mixture press-in die, make diameter 50.0cm, the column of high about 50.0cm, then drying forming at 100 DEG C, obtains capsule core.
Step 3: 4.0g agar and 5.0g sodium alginate are scattered in 100mL water, then heating makes agar and sodium alginate fully dissolve, then add 50g diatomite to mix, to be cooledly obtain capsule shell material to adding bacterium powder 2.0g after 50 DEG C and mixing, then for subsequent use at 45 DEG C.
Step 4: mixing capsule outer shell material step 3 prepared pours diameter 70.0m into, in the cylindrical mold of high 70.0cm.
Step 5: to be embedded into by step 2 gained capsule core in step 4 in capsule shell material, makes capsule core be positioned at mould central authorities and be flooded completely by capsule shell material.Then, under room temperature, cooled and solidified is shaping.
Step 6: taken out by the capsule of step 5 coagulation forming, immerses in the calcium chloride solution of 10% and solidifies 4h, then takes out to dry at 40 DEG C and spends the night, and namely obtains the nucleocapsid structure capsule embedding slow release carbon source.
This capsule is nucleocapsid structure, the maximum physical dimension of capsule core is 50.0cm, in kernel, each constituent mass percentage composition is Trisodium Citrate 70%, stearic acid 1.0%, calcium acetate 1.0%, sodium-acetate 1.0%, calcium chloride 25.7%, bamboo charcoal 0.1%, calcium carbonate 45.8%, xanthan gum 0.5%, sodium alginate 0.5%, sucrose 0.1%, and capsule shell thickness is 10.0cm; Kernel molding process, the mass ratio of material and water is 10:5, and bake out temperature is 100 DEG C; The each component concentration of shell is agar 4.0g in every 100mL water, sodium alginate 5.0g, diatomite 50.0g, bacterium powder 2.0g, and in shell, subtilis BJ and Bacillus licheniformis DSM 13 viable count are 1.0 × 10
9cFU/g weight in wet base capsule shell; Capsule shell solidifying agent to be mass percent concentration be 10% calcium chloride water, solidification duration is 4.0h, and microbial inoculum encapsulation rate is 99.0%, and cured glue capsule bake out temperature is 40 DEG C; The cycle that capsule core discharges 90% water-soluble carbon source is 48.0 months, and the cycle of release 85% embedding microbial inoculum is 57.0 months.
Embodiment 3
Step 1: by 55g Trisodium Citrate, 5g stearic acid, 1g calcium acetate, 1g sodium-acetate, 23.5g calcium chloride, 5.0g bamboo charcoal, 5.0g calcium carbonate, 2.0g xanthan gum, 2.0g sodium alginate and 0.5g sucrose mix.Then add 30g water to mix, be modulated into dough carbon source mixture.
Step 2: by dough carbon source mixture press-in die, make diameter 25.0cm, the column of high about 25.0cm, then drying forming at 75 DEG C, obtains capsule core.
Step 3: 2.0g agar and 2.5g sodium alginate are scattered in 100mL water, then heating makes agar and sodium alginate fully dissolve, then add 35g diatomite to mix, to be cooledly obtain capsule shell material to adding bacterium powder 1.0g after 50 DEG C and mixing, then for subsequent use at 45 DEG C.
Step 4: mixing capsule outer shell material step 3 prepared pours diameter 35.0m into, in the cylindrical mold of high 35.0cm.
Step 5: to be embedded into by step 2 gained capsule core in step 4 in capsule shell material, makes capsule core be positioned at mould central authorities and be flooded completely by capsule shell material.Then, under room temperature, cooled and solidified is shaping.
Step 6: taken out by the capsule of step 5 coagulation forming, immerses in the calcium chloride solution of 7.5% and solidifies 2h, then takes out to dry at 30 DEG C and spends the night, and namely obtains the nucleocapsid structure capsule embedding slow release carbon source.
This capsule is nucleocapsid structure, the maximum physical dimension of capsule core is 25.0cm, in kernel, each constituent mass percentage composition is Trisodium Citrate 55.0%, stearic acid 5.0%, calcium acetate 1.0%, sodium-acetate 1.0%, calcium chloride 23.5%, bamboo charcoal 5.0%, calcium carbonate 5.0%, xanthan gum 2.0%, sodium alginate 2.0%, sucrose 0.5%, and capsule shell thickness is 5.0cm; Kernel molding process, the mass ratio of material and water is 10:3, and bake out temperature is 75 DEG C; The each component concentration of shell is agar 2.0g in every 100mL water, sodium alginate 2.5g, diatomite 35.0g, bacterium powder 1.0g, and in shell, subtilis BJ and Bacillus licheniformis DSM 13 viable count are 1.0 × 10
8cFU/g weight in wet base capsule shell; Capsule shell solidifying agent to be mass percent concentration be 7.5% calcium chloride water, solidification duration is 2.0h, and microbial inoculum encapsulation rate is 95.7%, and cured glue capsule bake out temperature is 30 DEG C; The cycle that capsule core discharges 90% water-soluble carbon source is 37.0 months, and the cycle of release 85% embedding microbial inoculum is 39.0 months.
Embodiment 4
Step 1: by 53g Trisodium Citrate, 6g stearic acid, 10g calcium acetate, 2g sodium-acetate, 20g calcium chloride, 5g bamboo charcoal, 0.1g calcium carbonate, 2.4g xanthan gum, 1.0g sodium alginate and 0.5g sucrose mix.Then add 20g water to mix, be modulated into dough carbon source mixture.
Step 2: by dough carbon source mixture press-in die, make diameter 50.0cm, the column of high about 50.0cm, then drying forming at 90 DEG C, obtains capsule core.
Step 3: 1.4g agar and 2.0 sodium alginates are scattered in 100mL water, then heating makes agar and sodium alginate fully dissolve, then add 30g diatomite to mix, to be cooledly obtain capsule shell material to adding bacterium powder 2.0g after 50 DEG C and mixing, then for subsequent use at 45 DEG C.
Step 4: mixing capsule outer shell material step 3 prepared pours diameter 70.0m into, in the cylindrical mold of high 70.0cm.
Step 5: to be embedded into by step 2 gained capsule core in step 4 in capsule shell material, makes capsule core be positioned at mould central authorities and be flooded completely by capsule shell material.Then, under room temperature, cooled and solidified is shaping.
Step 6: taken out by the capsule of step 5 coagulation forming, immerses in the calcium chloride solution of 10% and solidifies 4h, then takes out to dry at 25 DEG C and spends the night, and namely obtains the nucleocapsid structure capsule embedding slow release carbon source.
This capsule is nucleocapsid structure, the maximum physical dimension of capsule core is 50.0cm, in kernel, each constituent mass percentage composition is Trisodium Citrate 53%, stearic acid 6%, calcium acetate 10%, sodium-acetate 2%, calcium chloride 20%, bamboo charcoal 5%, calcium carbonate 0.1%, xanthan gum 2.4%, sodium alginate 1% and sucrose 0.5%, and capsule shell thickness is 10.0cm; Kernel molding process, the mass ratio of material and water is 10:2, and bake out temperature is 90 DEG C; The each component concentration of shell is agar 1.4g in every 100mL water, sodium alginate 2.0g, diatomite 30.0g, bacterium powder 2.0g, and in shell, subtilis BJ and Bacillus licheniformis DSM 13 viable count are 1.0 × 10
9cFU/g weight in wet base capsule shell; Capsule shell solidifying agent to be mass percent concentration be 10% calcium chloride water, solidification duration is 4.0h, and microbial inoculum encapsulation rate is 99.0%, and cured glue capsule bake out temperature is 25 DEG C; The cycle that capsule core discharges 90% water-soluble carbon source is 60.0 months, and the cycle of release 85% embedding microbial inoculum is 70.0 months.
Embodiment 5
Step 1: by 40g Trisodium Citrate, 10g stearic acid, 20g calcium acetate, 5.0g sodium-acetate, 10g calcium chloride, 5.0g bamboo charcoal, 1.0g calcium carbonate, 4.0g xanthan gum, 4.0g sodium alginate and 1.0g sucrose mix.Then add 40g water to mix, be modulated into dough carbon source mixture.
Step 2: by dough carbon source mixture press-in die, make diameter 10.0cm, the column of high about 10.0cm, then drying forming at 60 DEG C, obtains capsule core.
Step 3: 1.0g agar and 3.0 sodium alginates are scattered in 100mL water, then heating makes agar and sodium alginate fully dissolve, then add 35g diatomite to mix, to be cooledly obtain capsule shell material to adding bacterium powder 1.0g after 50 DEG C and mixing, then for subsequent use at 45 DEG C.
Step 4: mixing capsule outer shell material step 3 prepared pours diameter 22.0m into, in the cylindrical mold of high 22.0cm.
Step 5: to be embedded into by step 2 gained capsule core in step 4 in capsule shell material, makes capsule core be positioned at mould central authorities and be flooded completely by capsule shell material.Then, under room temperature, cooled and solidified is shaping.
Step 6: taken out by the capsule of step 5 coagulation forming, immerses in the calcium chloride solution of 8% and solidifies 3h, then takes out to dry at 35 DEG C and spends the night, and namely obtains the nucleocapsid structure capsule embedding slow release carbon source.
This capsule is nucleocapsid structure, the maximum physical dimension of capsule core is 10.0cm, in kernel, each constituent mass percentage composition is Trisodium Citrate 20%, stearic acid 10%, calcium acetate 20%, sodium-acetate 10%, calcium chloride 10%, bamboo charcoal 20%, calcium carbonate 1.0%, xanthan gum 4.0%, sodium alginate 4.0% and sucrose 1.0%, and capsule shell thickness is 6.0cm; Kernel molding process, the mass ratio of material and water is 10:2, and bake out temperature is 60 DEG C; The each component concentration of shell is agar 1.0g in every 100mL water, sodium alginate 3.0g, diatomite 35.0g, bacterium powder 1.0g, and in shell, subtilis BJ and Bacillus licheniformis DSM 13 viable count are 3.4 × 10
8cFU/g weight in wet base capsule shell; Capsule shell solidifying agent to be mass percent concentration be 8% calcium chloride water, solidification duration is 3.0h, and microbial inoculum encapsulation rate is 97.1%, and cured glue capsule bake out temperature is 35 DEG C; The cycle that capsule core discharges 90% water-soluble carbon source is 19.5 months, and the cycle of release 85% embedding microbial inoculum is 20.0 months.
Embodiment 6
Step 1: by 40.0g Trisodium Citrate, 10g stearic acid, 4.0g calcium acetate, 1.0g sodium-acetate, 10.0g calcium chloride, 0.5g bamboo charcoal, 30.0g calcium carbonate, 2.0g xanthan gum, 2.0g sodium alginate and 0.5g sucrose mix.Then add 15g water to mix, be modulated into dough carbon source mixture.
Step 2: by dough carbon source mixture press-in die, make diameter 10.0cm, the column of high about 10.0cm, then drying forming at 60 DEG C, obtains capsule core.
Step 3: 1.0g agar and 3.0 sodium alginates are scattered in 100mL water, then heating makes agar and sodium alginate fully dissolve, then add 35g diatomite to mix, to be cooledly obtain capsule shell material to adding bacterium powder 1.0g after 50 DEG C and mixing, then for subsequent use at 45 DEG C.
Step 4: mixing capsule outer shell material step 3 prepared pours diameter 22.0m into, in the cylindrical mold of high 22.0cm.
Step 5: to be embedded into by step 2 gained capsule core in step 4 in capsule shell material, makes capsule core be positioned at mould central authorities and be flooded completely by capsule shell material.Then, under room temperature, cooled and solidified is shaping.
Step 6: taken out by the capsule of step 5 coagulation forming, immerses in the calcium chloride solution of 8% and solidifies 3h, then takes out to dry at 35 DEG C and spends the night, and namely obtains the nucleocapsid structure capsule embedding slow release carbon source.
This capsule is nucleocapsid structure, the maximum physical dimension of capsule core is 10.0cm, in kernel, each constituent mass percentage composition is Trisodium Citrate 40.0%, stearic acid 10.0%, calcium acetate 4.0%, sodium-acetate 1.0%, calcium chloride 10.0%, bamboo charcoal 0.5%, calcium carbonate 30.0%, xanthan gum 2.0%, sodium alginate 2.0% and sucrose 0.5%, and capsule shell thickness is 6.0cm; Kernel molding process, the mass ratio of material and water is 10:1.5, and bake out temperature is 60 DEG C; The each component concentration of shell is agar 1.0g in every 100mL water, sodium alginate 3.0g, diatomite 35.0g, bacterium powder 1.0g, and in shell, subtilis BJ and Bacillus licheniformis DSM 13 viable count are 3.0 × 10
8cFU/g weight in wet base capsule shell; Capsule shell solidifying agent to be mass percent concentration be 8% calcium chloride water, solidification duration is 3.0h, and microbial inoculum encapsulation rate is 96.4%, and cured glue capsule bake out temperature is 35 DEG C; The cycle that capsule core discharges 90% water-soluble carbon source is 25.5 months, and the cycle of release 85% embedding microbial inoculum is 34.5 months.
Embodiment 6
Step 1: by 40.0g Trisodium Citrate, 1.0g stearic acid, 1.0g calcium acetate, 10.0g sodium-acetate, 10.0g calcium chloride, 20.0g bamboo charcoal, 13.5g calcium carbonate, 2.0g xanthan gum, 2.0g sodium alginate and 0.5g sucrose mix.Then add 30g water to mix, be modulated into dough carbon source mixture.
Step 2: by dough carbon source mixture press-in die, make diameter 7.0cm, the column of high about 7.0cm, then drying forming at 75 DEG C, obtains capsule core.
Step 3: 2.0g agar and 1.0 sodium alginates are scattered in 100mL water, then heating makes agar and sodium alginate fully dissolve, then add 40g diatomite to mix, to be cooledly obtain capsule shell material to adding bacterium powder 1.0g after 50 DEG C and mixing, then for subsequent use at 45 DEG C.
Step 4: mixing capsule outer shell material step 3 prepared pours diameter 11.0m into, in the cylindrical mold of high 11.0cm.
Step 5: to be embedded into by step 2 gained capsule core in step 4 in capsule shell material, makes capsule core be positioned at mould central authorities and be flooded completely by capsule shell material.Then, under room temperature, cooled and solidified is shaping.
Step 6: taken out by the capsule of step 5 coagulation forming, immerses in the calcium chloride solution of 8% and solidifies 4h, then takes out to dry at 30 DEG C and spends the night, and namely obtains the nucleocapsid structure capsule embedding slow release carbon source.
This capsule is nucleocapsid structure, the maximum physical dimension of capsule core is 7.0cm, in kernel, each constituent mass percentage composition is Trisodium Citrate 40.0%, stearic acid 1.0%, calcium acetate 1.0%, sodium-acetate 10.0%, calcium chloride 10.0%, bamboo charcoal 20.0%, calcium carbonate 13.5%, xanthan gum 2.0%, sodium alginate 2.0% and sucrose 0.5%, and capsule shell thickness is 2.0cm; Kernel molding process, the mass ratio of material and water is 10:3, and bake out temperature is 75 DEG C; The each component concentration of shell is agar 2.0g in every 100mL water, sodium alginate 1.0g, diatomite 40.0g, bacterium powder 1.0g, and in shell, subtilis BJ and Bacillus licheniformis DSM 13 viable count are 2.7 × 10
8cFU/g weight in wet base capsule shell; Capsule shell solidifying agent to be mass percent concentration be 8% calcium chloride water, solidification duration is 4.0h, and microbial inoculum encapsulation rate is 94.7%, and cured glue capsule bake out temperature is 30 DEG C; The cycle that capsule core discharges 90% water-soluble carbon source is 5.5 months, and the cycle of release 85% embedding microbial inoculum is 10.0 months.
Claims (4)
1. embed water purification microbial inoculum to ease up and release a nucleocapsid structure capsule for carbon source, it is characterized in that: capsule is nucleocapsid structure, to mix slow release carbon source for core, with microbial inoculum and Organic-inorganic composite porous material for shell, wherein, the core of nucleocapsid structure capsule is mixing slow release carbon source, it is characterized in that, this core is by Trisodium Citrate, stearic acid, calcium acetate, sodium-acetate, calcium chloride, bamboo charcoal, calcium carbonate, xanthan gum, sodium alginate and sucrose etc. mix, in mixture, the mass percentage content of Trisodium Citrate is 40 ~ 70%, stearic mass percentage content is 1 ~ 10%, the mass percentage content of calcium acetate is 1 ~ 20%, the mass percentage content of sodium-acetate is 1 ~ 10%, the mass percentage content of calcium chloride is 10 ~ 40%, the mass percentage content of bamboo charcoal is 0.1 ~ 20%, the mass percentage content of calcium carbonate is 0.1 ~ 30%, the mass percentage content of xanthan gum is 0.5 ~ 4%, the mass percentage content of sodium alginate be 0.5 ~ 4% and the mass percentage content of sucrose be 0.1 ~ 1%.
2. nucleocapsid structure capsule according to claim 1, wherein, the shell of nucleocapsid capsule is microbial inoculum and Organic-inorganic composite porous material, it is characterized in that: shell is mixed in proportion by bacterium powder, diatomite, agar and sodium alginate and is dissolved or dispersed in water and forms; Wherein, microbial inoculum 0.2 ~ 2.0g in every 100mL water, agar 0.5 ~ 4.0g, sodium alginate 0.5 ~ 5.0g, diatomite 20 ~ 50g; Microbial inoculum is mixed according to bacterium number by subtilis BJ bacterium powder and Bacillus licheniformis DSM 13 bacterium powder, and the two viable count is 1.0 × 10
7~ 1.0 × 10
9cFU/g weight in wet base capsule shell.
3. nucleocapsid structure capsule according to claim 1, wherein, the profile of described nucleocapsid structure capsule can be ball-type, square, column, strip, wire or pie etc., and the maximum physical dimension of capsule core is 1.0cm ~ 50cm, and the thickness of capsule shell is 0.2 ~ 10cm.
4. a preparation method for nucleocapsid structure water purification bacterium agent capsules, comprises the following steps:
The first step, configuration capsule core material, i.e. slow release carbon source mixture: by Trisodium Citrate, stearic acid, calcium acetate, sodium-acetate, calcium chloride, bamboo charcoal, calcium carbonate, xanthan gum, sodium alginate and sucrose mix in proportion, wherein the mass percentage content of Trisodium Citrate is 40 ~ 70%, stearic mass percentage content is 1 ~ 10%, the mass percentage content of calcium acetate is 1 ~ 20%, the mass percentage content of sodium-acetate is 1 ~ 10%, the mass percentage content of calcium chloride is 10 ~ 40%, the mass percentage content of bamboo charcoal is 0.1 ~ 20%, the mass percentage content of calcium carbonate is 0.1 ~ 30%, the mass percentage content of xanthan gum is 0.5 ~ 4%, the mass percentage content of sodium alginate be 0.5 ~ 4% and the mass percentage content of sucrose be 0.1 ~ 1%,
Second step, capsule core makes shaping: mixed according to mass ratio=10:1 ~ 10:5 with water by the mixture of the first step, make dough or pasty material, and be processed into desired shape, then dries in 50 ~ 100 DEG C and obtains moulding capsules kernel;
3rd step, capsule shell material prepares: 0.5 ~ 4.0g agar, 0.5 ~ 5.0g sodium alginate and 20 ~ 50g diatomite are joined in 100mL water, is heated to dissolve fully; When temperature is reduced to 50 DEG C, add subtilis BJ bacterium powder 0.2 ~ 2.0g and Bacillus licheniformis DSM 13 bacterium powder 0.1 ~ 2.0g, and the acquisition outer shell material that stirs rapidly, for subsequent use at 45 DEG C;
4th step, prepared by nucleocapsid capsule: pour in the mould of definite shape by the outer shell material that the 3rd step obtains, and then the moulding capsules kernel that second step obtains is embedded outer shell material inner, kernel is submerged in outer shell material completely, cooling forming under room temperature:
5th step, capsule shell solidification and capsule drying treatment: the capsule of the 4th step cooling forming is immersed 0.5 ~ 4h in 5 ~ 10% calcium chloride solutions, then take out oven dry at 20 ~ 40 DEG C and spend the night and can obtain the final nucleocapsid structure capsule of the present invention.
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