CN104897747A - 一种胰岛素的酶免疫测定法 - Google Patents

一种胰岛素的酶免疫测定法 Download PDF

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CN104897747A
CN104897747A CN201510274996.XA CN201510274996A CN104897747A CN 104897747 A CN104897747 A CN 104897747A CN 201510274996 A CN201510274996 A CN 201510274996A CN 104897747 A CN104897747 A CN 104897747A
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insulin
minutes
enzyme immunoassay
antibody
electrode
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任磊
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Hefei Zhuoyuan Technology Service Co Ltd
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Hefei Zhuoyuan Technology Service Co Ltd
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Abstract

本发明公开了一种胰岛素的酶免疫测定法，涉及酶免疫测量领域。为了简便有效的测定血液中胰岛素的浓度，本发明主要包括抗体膜的氧电极置于含有胰岛素的溶液中，30度的条件下温育10分钟，除去非特异结合的物质，加入一定量的过氧化氢，制作标准曲线，抗体再生。本发明抗体膜与胰岛素之间的相互作用对产生电流的影响，简便有效的对血液中胰岛素的浓度进行测定。

Description

一种胰岛素的酶免疫测定法
技术领域
    本发明涉及酶免疫测量领域，尤其涉及一种胰岛素的酶免疫测定法。
背景技术
胰岛素对物质代谢的作用非常重要，总的效果是促进合成代谢，抑制分解代谢。若缺少胰岛素，则血糖升高。因此，测定血液中胰岛素浓度和血糖值对诊断、治疗糖尿病是很重要的。
发明内容
本发明要解决以上的技术问题，提供一种胰岛素的酶免疫测定法，来简便有效的测定血液中胰岛素的浓度。
为解决上述技术问题，本发明是通过以下技术方案实现的：
一种胰岛素的酶免疫测定法：
（1）、将装有抗体膜的氧电极置于含有胰岛素的溶液中；
（2）、再置于30度的条件下温育10分钟；
（3）、温育10分钟后再放入pH8缓冲液中浸泡5分钟除去非特异结合的物质；
（4）、再加入一定量的过氧化氢；
（5）、用记录仪记录加入底物后电流值的增加，求出电流增加的初始速度，将它对胰岛素浓度作图制作标准曲线；
（6）、测定完毕后将电极置于pH2.2，0.2mol/L的甘氨酸-HCl溶液中10分钟使电极上抗体再生。
与现有的技术相比，本发明的有益效果是：本发明抗体膜与胰岛素之间的相互作用对产生电流的影响，简便有效的对血液中胰岛素的浓度进行测定。
具体实施方式
为了使本发明的目的、技术方案及优点更加清楚明白，以下对本发明进行进一步详细说明。应当理解，此处所描述的具体实施例仅仅用以解释本发明，并不用于限定本发明。
本发明提供一种胰岛素的酶免疫测定法：
（1）、将装有抗体膜的氧电极置于含有胰岛素的溶液中；
（2）、再置于30度的条件下温育10分钟；
（3）、温育10分钟后再放入pH8缓冲液中浸泡5分钟除去非特异结合的物质；
（4）、再加入一定量的过氧化氢；
（5）、用记录仪记录加入底物后电流值的增加，求出电流增加的初始速度，将它对胰岛素浓度作图制作标准曲线；
（6）、测定完毕后将电极置于pH2.2，0.2mol/L的甘氨酸-HCl溶液中10分钟使电极上抗体再生。
以上所述仅为本发明的较佳实施例而已，并不用以限制本发明，凡在本发明的精神和原则之内所作的任何修改、等同替换和改进等，均应包含在本发明的保护范围之内。

Claims (1)

1.一种胰岛素的酶免疫测定法，其特征在于：
（1）、将装有抗体膜的氧电极置于含有胰岛素的溶液中；
（2）、再置于30度的条件下温育10分钟；
（3）、温育10分钟后再放入pH8缓冲液中浸泡5分钟除去非特异结合的物质；
（4）、再加入一定量的过氧化氢；
（5）、用记录仪记录加入底物后电流值的增加，求出电流增加的初始速度，将它对胰岛素浓度作图制作标准曲线；
（6）、测定完毕后将电极置于pH2.2，0.2mol/L的甘氨酸-HCl溶液中10分钟使电极上抗体再生。
CN201510274996.XA 2015-05-27 2015-05-27 一种胰岛素的酶免疫测定法 Pending CN104897747A (zh)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63117253A (ja) * 1986-11-05 1988-05-21 Kanebo Ltd 免疫センサ−
JP2008082987A (ja) * 2006-09-28 2008-04-10 Hokkaido Univ インスリンの検出装置
CN103454433A (zh) * 2012-06-05 2013-12-18 许洋 一种新型检测内外源性胰岛素的免疫质谱试剂盒

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63117253A (ja) * 1986-11-05 1988-05-21 Kanebo Ltd 免疫センサ−
JP2008082987A (ja) * 2006-09-28 2008-04-10 Hokkaido Univ インスリンの検出装置
CN103454433A (zh) * 2012-06-05 2013-12-18 许洋 一种新型检测内外源性胰岛素的免疫质谱试剂盒

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JAMES A. COX ET AL.: "Flow Injection Amperometric Determination of Insulin Based upon Its Oxidation at a Modified Electrode", 《ANAL. CHEM》 *
铃木周一: "《生物传感器》", 31 December 1988 *

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