CN104897635B - Immunofluorescence analysis instrument - Google Patents
Immunofluorescence analysis instrument Download PDFInfo
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- CN104897635B CN104897635B CN201510349446.XA CN201510349446A CN104897635B CN 104897635 B CN104897635 B CN 104897635B CN 201510349446 A CN201510349446 A CN 201510349446A CN 104897635 B CN104897635 B CN 104897635B
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Abstract
The present invention provides a kind of immunofluorescence analysis instrument, includes scan control circuit, mechanical driving device, light source, excitation light path, fluorescence detection optical path, integrated amplifier, optical-electrical converter, the control unit being sequentially connected；Sample batch processing device is also included, sample batch processing device includes example reaction part and sample-adding process part, and sample-adding process part includes support member, flow control member, open and close controlling part.A kind of immunofluorescence analysis instrument that the present invention is provided, by using the structure based on coaxial focusing principle altogether, specific sample batch processing device is provided, and instrument component corresponding with other coordinates, can quickly, while accurately analyzing a series of, big batch test antibodies or antigenic substance, it is especially suitable for accident scene and basic unit uses, and may be used on clinical detection, for clinical treatment provides qualitative, quantitative foundation.
Description
Technical field
The invention belongs to analyze testing equipment technical field, and in particular to a kind of immunofluorescence analysis instrument.
Background technology
Immunofluorescence technique (Immunofluorescence assay, IFA) was originated in early 1940s, by
Coons etc. checks that the soluble pneumococal polysaccharide in mouse tissue section resists using fluorescein labelled antibody first in nineteen forty-one
Original simultaneously succeeds.Its measuring principle is：With the antibody or antigen and sample (cell, tissue or separate material of fluorescence labeling
Deng) in corresponding antigen or antibody combine, it is analyzed with the technology for suitably detecting fluorescence.Wherein, due to fluorchrome
Can not only be combined with antibody globulin, for detecting or positioning various antigens, it is also possible to combined with other protein, for detecting
Or positioning antibody.Therefore, the technology referred to as fluorescent antibody technics of antigen positioning is carried out with fluorescent material labelled antibody
(fluorescent antibody technique), with the spike of known fluorescent antigen label or the side of inspection corresponding antibodies
Method claims fluorescent antigen technology, both approaches general name immunofluorescence technique.But fluorescent antigen technology is little in real work
Using so fluorescent antibody technics is referred to as immunofluorescence technique by people's custom.
Since 20 century 70s, on the basis of conventional fluorescent antibody technique, development establishes various fluorescence immunoassays and surveys
Determine method (fluorescence immunoassay, FIA) to can be used to quantitative determine the antigen or antibody in body fluid, so that immune
Fluorescence analysis enter a new developing stage.Fluorescence immunoassay is after antigen-antibody reaction, to be surveyed using instrument
Determine fluorescence intensity and calculate the detection method of measured object concentration.It is set to due to the specific of FIA, rapidity and in cellular level
Accuracy, the new discovery of fluorescent material and updating for labeling method, IFA is in immunology, microbiology, pathology, swollen
Many aspects such as knurl and clinical examination are widely used.
At present, the analytical instrument that fluorescence immunoassay is used is immunofluorescence analysis instrument, and it is according to the reflection of solid phase light
Colorimetric principle, according to the label of luciferase assay reagent --- fluorescent grain is inspired under the laser excitation of near-infrared wavelength
Fluorescence signal, and kit detection line (T lines) fluorescence signal intensity and sample concentration follow certain corresponding relation and
The analytical instrument of design.
But, a sample can only be detected simultaneously for traditional immunofluorescence analysis instrument and reliability, stability and
Poor sensitivity, complex operation is inconvenient to carry, it can be seen that, traditional immunofluorescence analysis instrument also needs in the presence of certain defect
Improve, be highly desirable to make standardization, quantification and the automation of immunofluorescence analysis instrument to be further improved.
The content of the invention
The shortcoming of prior art, it is an object of the invention to provide a kind of immunofluorescence analysis instrument, uses in view of the above
In solve in the prior art lack can carry out batch detection and batch sample testing result reliability, stability and sensitivity it is good
Immunofluorescence analysis instrument problem.
In order to achieve the above objects and other related objects, the present invention provides a kind of immunofluorescence analysis instrument, includes successively
The scan control circuit of connection, mechanical driving device, light source, excitation light path, fluorescence detection optical path, integrated amplifier, photoelectricity turn
Parallel operation, control unit；Sample batch processing device is also included, the sample batch processing device includes following part：
Example reaction part, the example reaction part includes being sequentially connected adding mouth, sample cell, the sample introduction being integrated
Manage, go out sample pipe, reagent strip room, the reagent strip room includes reagent strip chamber, wherein, the sample cell side and the adding mouth
It is connected, the one end of the sample cell opposite side respectively with least two sample feeding pipes is connected, the other end warp of the sample feeding pipe
Go out sample pipe to be connected with reagent strip chamber one end；
Sample-adding process part, the sample-adding process part includes support member, flow control member,
Wherein, the flow control member includes hollow stent and roller, and the support is respectively provided on two sides with symmetrical
A pair of tracks, the track includes the first oblique orbit, the second oblique orbit, horizontal rail, and first oblique orbit is through level
Track is connected with second oblique orbit；The roller includes the first roller and the second roller, the center of first roller
Position is provided with the first roller bearing, and the center of second roller is provided with the second roller bearing, the roller through roller bearing with it is the pair of
Track rolls connection；
The leg detachable connection of the support member and the hollow stent directly over it；
Process part is detachable is socketed on outside the sample feeding pipe of the example reaction part for the sample-adding, in socket state
Under, first roller, the second roller are located on the sample feeding pipe, and perpendicular with the sample feeding pipe.
The example reaction part is single use part.Using rear discardable.
Preferably, height of the height of the adding mouth higher than the sample cell.
Preferably, the adding mouth is in lower cancave cambered surface near sample feeding pipe side with the sample cell.The shape and structure is favourable
Filled in sample in guarantee sample feeding pipe.
It is highly preferred that the radian of the lower cancave cambered surface is 90-120 °.
Preferably, reagent strip chamber rear and front end is respectively provided on two sides with reagent strip fixed block.The reagent strip is fixed
Block being capable of effective fixating reagent bar.
Preferably, the example reaction part also includes code room, and the code room includes coding chamber and encoding strip,
The encoding strip is covered in coding chamber.The encoding strip includes batch coding, individual number encoder, the batch coding and number
Coding is arranged in order, and the batch coding can effectively recognize the batch and numbering number of sample with a number encoder.
It is highly preferred that being additionally provided with barrier film in the coding chamber, the barrier film is covered on the encoding strip.In batch
Barrier film is pasted on coding and a number encoder, is prevented batch from encoding and is come off with a number encoder；When needed, can easily by barrier film
Removed with encoding strip and changed.
Most preferably, the barrier film is transparent and impermeable material.The barrier film is transparent non-setting adhesive band.Protection is compiled
Code-bar avoids reagent on reagent strip from staiing.
Preferably, the caliber of the sample feeding pipe goes out the caliber of sample pipe more than described in.Be conducive in sample feeding pipe batches of sample point
Other accurate quantitative analysis are from the reagent strip gone out during sample pipe is added dropwise to reagent strip chamber.
It is highly preferred that it is described go out sample pipe caliber be the sample feeding pipe caliber 1/3-1/4.
Preferably, the support member is that detachable draw-in groove connects with the leg of the hollow stent directly over it
Connect.
Preferably, the sample-adding process part also includes open and close controlling part.
It is highly preferred that the open and close controlling part includes contiguous block, rotary shaft, depression bar, horizontal block, spring, the connection
Block side is connected with the support member, and the contiguous block opposite side is connected with the horizontal block, and the contiguous block inside is also
Rotary shaft is provided with, the rotary shaft side is connected with the support, and the rotary shaft opposite side is connected with the depression bar, institute
State and be additionally provided with spring between depression bar and the horizontal block.The open and close controlling part can control opening for support member and flow control member
Close.
Preferably, it is additionally provided with dividing plate on the support.The dividing plate can control, separate different sample feeding pipes on support member
Position.
Preferably, the central point of the horizontal rail is equal or slightly larger than the roller to the vertical range of support member top surface
Radius.
It is highly preferred that vertical range radius more than the roller of the central point of the horizontal rail to support member top surface
0.05-0.1cm。
Preferably, the length of the horizontal rail is the 1/2-2/3 of the horizontal length of the support member.
The scan control circuit, mechanical driving device, light source, excitation light path, fluorescence detection optical path use coaxial copolymerization
Burnt light channel structure.The scan control circuit uses pulsewidth modulation (PWM) principle, bipolar DC Chopper driving mode, serial control
The start-stop of mechanical driving device processed, rotating speed and direction.The mechanical driving device drives light under the driving of scan control circuit
Source carries out progressive scan transmitting fluorescence to the reagent strip room in sample batch processing device and code room.
Preferably, the light source is laser diode, and the wavelength of the laser diode is 600-650nm.In this scope
Interior, fluorescent marker has maximum excitation light in labeling pad in reagent strip.
It is highly preferred that the wavelength of the laser diode is 630nm.
Preferably, the excitation light path includes spectroscope, the lens being sequentially connected.
Preferably, the fluorescence detection optical path includes optical filter, lens, the grating being sequentially connected.
The integrated amplifier is that the fluorescence signal that will be inspired amplifies.
The optical-electrical converter (i.e. A/D adapters) is that fluorescence signal is converted into electric signal.
Described control unit has the function of single-chip microcomputer and PC, and the electric signal that will can be received carries out data processing, leads to
Crossing makes to form specific functional relation between testing sample concentration and absorbance within the specific limits and discharge interference, is exported after analysis
Testing result.Increase batch discrepancy adjustment system in described control unit, effectively solve the problems, such as the difference between batch of testing sample.
Preferably, described control unit is also circumscribed with interface display module, data and uploads and print module.
It is highly preferred that the interface display module is liquid crystal display and touch-screen.
It is highly preferred that the data are uploaded and print module is keyboard, printer, USB device.
The immunofluorescence analysis instrument is AD values 0-10000 to the detection range of fluorescence signal.
The present invention still further provides a kind of purposes of immunofluorescence analysis instrument in immunofluorescence analysis field.
The present invention furthermore provides a kind of application method of immunofluorescence analysis instrument, specifically includes following steps：
1) sample-adding process part horizontal positioned is taken, flow control member is opened, the sample feeding pipe of example reaction part is put into, closed
After conjunction, then the reagent strip of batch processing is respectively put into reagent strip room, and promote the second oblique orbit on the second roller, under be rolled to
Horizontal rail, blocks sample feeding pipe outlet；
Preferably, second roller is rolled by the second roller bearing in second oblique orbit.
Preferably, the reagent strip is fixed in reagent strip chamber by reagent strip fixed block.
2) after adding adding mouth, sample to separately flow into sample feeding pipe through sample cell in sample, the on the first oblique orbit is promoted
One roller, under be rolled to horizontal rail, block sample feeding pipe import；
Preferably, first roller is rolled by the first roller bearing in first oblique orbit.
3) the second roller in horizontal rail, the second oblique orbit of above rolling back are promoted, and promotes the first roller in horizontal rail
Upper rolling, sample extrusion in sample feeding pipe and being added drop-wise on reagent strip through going out sample pipe is reacted；
4) light source is passed through to reagent in reagent strip room by excitation light path by scan control circuit control machinery transmission device
Encoding strip carries out progressive scan transmitting fluorescence in bar and code room, then produces fluorescence signal by fluorescence detection optical path, through integrated
Amplifier amplifies, and optical-electrical converter is converted to electric signal, data processing is carried out in control unit；
5) after detection is finished, flow control member is opened, changes example reaction part, new reagent strip is placed into, in repetition
Step is stated, proceeds sample batch detection.
As described above, a kind of immunofluorescence analysis instrument that the present invention is provided, by using based on coaxial focusing principle altogether
Structure, there is provided specific sample batch processing device, and instrument component corresponding with other cooperation, can be quick, accurate, qualitative fixed
The analysis of amount is a series of, big batch test antibodies or antigenic substance.Particularly batch processing sample can be accurately loaded together, batch
The sample-adding amount for processing sample is precisely consistent.Sample is not easy to spill in the device simultaneously, so as to avoid because caused by sample loss
Detection error.It can be seen that, immunofluorescence analysis instrument sample collection and treatment in detection process is simple, and sample can be carried out
Effective batch processing, batch detection, easy to detect, low cost, sensitivity and precision are high, and specificity is good, meanwhile, detection data can
Deposit readable, high degree of automation can fast and accurately obtain result, be especially suitable for accident scene and basic unit uses, and can
It is applied in clinical detection, for clinical treatment provides qualitative, quantitative foundation.
Brief description of the drawings
Fig. 1 is shown as a kind of analysis process figure of immunofluorescence analysis instrument of the invention.
Fig. 2 is shown as a kind of control unit annexation schematic diagram of immunofluorescence analysis instrument of the invention.
Fig. 3 is shown as a kind of planar structure schematic diagram of the sample batch processing device of immunofluorescence analysis instrument of the invention.
Fig. 4 is shown as a kind of planar structure schematic diagram of the example reaction part of immunofluorescence analysis instrument of the invention.
Fig. 5 is shown as a kind of planar structure schematic diagram of the sample-adding process part of immunofluorescence analysis instrument of the invention.
Fig. 6 is shown as a kind of side structure schematic diagram of the sample batch processing device of immunofluorescence analysis instrument of the invention.
Fig. 7 is shown as a kind of cross-sectional structure schematic diagram of the sample-adding process part of immunofluorescence analysis instrument of the invention.
Reference
1 scan control circuit
2 mechanical driving devices
3 light sources
4 excitation light paths
5 sample batch processing devices
51 example reaction parts
511 adding mouths
512 sample cells
513 sample feeding pipes
514 go out sample pipe
515 reagent strip rooms
5151 reagent strip chambers
5152 reagent strip fixed blocks
516 code rooms
5161 coding chambers
5162 encoding strips
5163 barrier films
52 sample-adding process parts
521 support members
522 flow control members
5221 supports
5222 tracks
The oblique orbits of a first
The oblique orbits of b second
C horizontal rails
5223 first rollers
5224 first roller bearings
5225 second rollers
5226 second roller bearings
5227 dividing plates
523 open and close controlling parts
5231 contiguous blocks
5232 rotary shafts
5233 depression bars
5234 horizontal blocks
5235 springs
6 fluorescence detection optical paths
7 integrated amplifiers
8 optical-electrical converters
9 control units
Specific embodiment
Embodiments of the present invention are illustrated below by way of specific instantiation, those skilled in the art can be by this specification
Disclosed content understands other advantages of the invention and effect easily.The present invention can also be by specific realities different in addition
The mode of applying is embodied or practiced, the various details in this specification can also based on different viewpoints with application, without departing from
Various modifications or alterations are carried out under spirit of the invention.
Before the specific embodiment of the invention is further described, it should be appreciated that protection scope of the present invention is not limited to down
State specific specific embodiment；It is also understood that the term used in the embodiment of the present invention is specific specific in order to describe
Embodiment, rather than in order to limit the scope of the invention；In description of the invention and claims, unless in text
Explicitly point out in addition, singulative " one ", " one " and " this " include plural form.
When embodiment provides number range, it should be appreciated that except non-invention is otherwise noted, two ends of each number range
Any one numerical value can select between point and two end points.Unless otherwise defined, in the present invention all technologies for using and
Scientific terminology is identical with the meaning that those skilled in the art of the present technique are generally understood that.Except the specific method, equipment used in embodiment,
Outside material, grasp and record of the invention according to those skilled in the art to prior art can also be used and this
Any method, equipment and material in the similar or equivalent prior art of method, equipment described in inventive embodiments, material come real
The existing present invention.
Meanwhile, refer to Fig. 1 to Fig. 7.It should be clear that structure, ratio, size depicted in this specification institute accompanying drawings etc.,
Only it is used to coordinate the content disclosed in specification, so that those skilled in the art understands and reads, is not limited to this hair
Bright enforceable qualifications, therefore do not have technical essential meaning, the modification of any structure, the change of proportionate relationship or size
Adjustment, in the case where of the invention effect that can be generated and the purpose to be reached is not influenceed, all should still fall disclosed
Technology contents obtain and can cover in the range of.Meanwhile, in this specification it is cited as " on ", D score, "left", "right", " in
Between " and " one " etc. term, be merely convenient to understanding for narration, and be not used to limit enforceable scope of the invention, its is relative
Relation is altered or modified, under without essence change technology contents, when being also considered as enforceable category of the invention.
As shown in Figure 1-2, the present invention provides a kind of immunofluorescence analysis instrument, includes the scan control circuit being sequentially connected
1st, mechanical driving device 2, light source 3, excitation light path 4, fluorescence detection optical path 6, integrated amplifier 7, optical-electrical converter 8, control are single
Unit 9, also includes sample batch processing device 5.
Scan control circuit 1, mechanical driving device 2, light source 3, excitation light path 4, fluorescence detection optical path 6, integrated amplifier
7th, optical-electrical converter 8, control unit 9 are prior art.Specific annexation is as shown in Figure 1.
Wherein, light source 3 is laser diode, and the wavelength of laser diode is 600-650nm, preferably 630nm.In this ripple
In scope long, there is maximum excitation light to fluorescent marker in labeling pad in reagent strip.Excitation light path 4 includes what is be sequentially connected
Spectroscope, lens, can effectively by exciting light be irradiated in the reagent strip room 515 in sample batch processing device 5 reagent strip and
In code room 516 on encoding strip 5162.Fluorescence detection optical path 6 includes optical filter, lens, the grating being sequentially connected, can be by
Fluorescence produces fluorescence signal after irradiation, and is transferred in integrated amplifier 7.The fluorescence signal that integrated amplifier 7 will be received amplifies,
And by optical-electrical converter (i.e. A/D adapters) be that fluorescence signal is converted into electric signal, it is input into control unit 9.
As shown in Fig. 2 control unit 9 is also circumscribed with interface display module, data uploading and print module.Interface display mould
Block is liquid crystal display and touch-screen, and data are uploaded and print module is keyboard, printer, USB device.Control unit 9 has monolithic
The function of machine and PC, the electric signal that will can be received carries out data processing, by make between testing sample concentration and absorbance
Specific functional relation is formed in certain limit and interference is discharged, testing result is exported after analysis.
As shown in figure 1, sample batch processing device 5 includes example reaction part 51 and sample-adding process part 52.Sample is anti-
It is split-type structural to answer part 51 and sample-adding process part 52.
As shown in Fig. 3,4,6, example reaction part 51 include be sequentially connected be integrated adding mouth 511, sample cell 512,
Sample feeding pipe 513, go out sample pipe 514, reagent strip room 515, reagent strip room 515 includes reagent strip chamber 5151, wherein, sample cell 512
Side is connected with adding mouth 511, and the one end of the opposite side of sample cell 512 respectively with least two sample feeding pipes 513 is connected, sample introduction
The other end of pipe 513 is connected through going out sample pipe 514 with the one end of reagent strip chamber 5151.Example reaction part 51 is single use portion
Part, using rear discardable.
Wherein, as shown in Figure 3,4, reagent strip chamber 5151 rear and front end is respectively provided on two sides with reagent strip fixed block 5152,
Being capable of effective fixating reagent bar.As shown in Fig. 3,4,6, example reaction part 51 also includes code room 516, and code room 516 is wrapped
Coding chamber 5161 and encoding strip 5162 are included, encoding strip 5162 is covered in coding chamber 5161；Coding chamber 5161 in be additionally provided with every
From film 5163, barrier film 5163 is covered on encoding strip 5162.Encoding strip 5162 includes batch coding, individual number encoder, described
Batch is encoded and a number encoder is arranged in order, and the batch coding can effectively recognize the batch and numbering of sample with a number encoder
Number.Barrier film 5163 is pasted on batch coding and a number encoder, prevents batch coding and the number encoder from coming off；When needed,
Easily barrier film 5163 and encoding strip 5162 can be removed and changed.Barrier film 5163 is transparent and impermeable material, specially
Transparent non-setting adhesive band.
As shown in fig. 6, height of the height of adding mouth 511 higher than sample cell 512；Adding mouth 511 is close to sample cell 512
The side of sample feeding pipe 513 is in lower cancave cambered surface, and the radian of lower cancave cambered surface is 90-120 °.When the shape and structure advantageously ensures that sample-adding, sample
Product flow into sample cell 512 from adding mouth 511, then flow into sample feeding pipe 513, it is ensured that sample is filled in sample feeding pipe 513.
As shown in Fig. 3,4,6, the caliber of sample feeding pipe 513 is more than the caliber of sample pipe 514.Due to going out the caliber of sample pipe 514
It is small, be conducive to will in sample feeding pipe 513 batch sample respectively accurate quantitative analysis from the reagent gone out during sample pipe 514 is added dropwise to reagent strip chamber 5151
On bar.It is the 1/3-1/4 of the caliber of sample feeding pipe 513 specifically to go out the caliber of sample pipe 514.
As shown in Fig. 5,6,7, sample-adding process part 52 includes support member 521, flow control member 522.
Wherein, as shown in Fig. 3,5,6,7, flow control member 522 includes hollow stent 5221 and roller, 5221 liang of support
Side is respectively equipped with symmetrical a pair of tracks 5222, and track 5222 includes the first oblique orbit a, the second oblique orbit b, horizontal rail
Road c, the first oblique orbit a is connected through horizontal rail c with the second oblique orbit b；Roller includes the first roller 5223 and the second roller
5225, the center of the first roller 5223 is provided with the first roller bearing 5224, and the center of the second roller 5225 is provided with the second rolling
Axle 5226, roller is rolled with a pair of tracks 5222 through roller bearing 5224,5226 and is connected.
Rolled from the first oblique orbit a to horizontal rail c by the first roller bearing 5224 of the center of the first roller 5223,
The import of multiple sample feeding pipes 513 can be effectively blocked, and sample pushing in multiple sample feeding pipes 513 is exported into warp by sample feeding pipe 513
On the reagent strip gone out during sample pipe 514 is added dropwise to multiple reagent strip chambers 5151 respectively.Meanwhile, by the centre bit of the second roller 5225
The second roller bearing 5226 put is rolled from the second oblique orbit b to horizontal rail c, can effectively block the outlet of multiple sample feeding pipes 513,
Play a part of valve.Simultaneously can be added drop-wise on reagent strip the sample in multiple sample feeding pipes 513 simultaneously by the structure, and be passed through
The dripping quantity of the first roller 5223 and the multiple samples of the second roller 5225 pairs is controlled, it is ensured that from sample feeding pipe 513 through going out sample pipe
514 to be added dropwise to sample size on the reagent strip in multiple reagent strip chambers 5151 respectively consistent.
As shown in Figure 6,7, the leg detachable connection of support member 521 and the hollow stent 5221 directly over it.Two
The detachable connection of person is preferably detachable draw-in groove connection.
In addition, as shown in fig. 7, sample-adding process part 52 also include open and close controlling part 523, open and close controlling part 523 includes
There are contiguous block 5231, rotary shaft 5232, depression bar 5233, horizontal block 5234, spring 5235, the side of contiguous block 5231 and support member
521 are connected, and the opposite side of contiguous block 5231 is connected with horizontal block 5234, and rotary shaft 5232 is additionally provided with inside contiguous block 5234,
The side of rotary shaft 5232 is connected with support 5221, and the opposite side of rotary shaft 5232 is connected with depression bar 5233, depression bar 5233 and water
Spring 5235 is additionally provided between plain block 5234.Open and close controlling part 523 can effectively control support member 521 and flow control member 522
Opening and closing close.Operationally, by by down-pressed pole 5233, under the effect of rotary shaft 5232, compressing depression bar 5233 and horizontal block
Spring 5235 between 5234, makes lifting for the medium-height trestle 5221 of flow control member 522；Decontrol depression bar 5233, depression bar 5233 and water
Spring 5235 between plain block 5234 restores to the original state, and promotes depression bar 5233 to lift, and makes the rotary shaft 5232 being connected with depression bar 5233
Support 5221 is driven to be closed with support member 521.
As Figure 1-3, generally, example reaction part 51 and sample-adding process part 52 are split-type structural.When
When being tested, example reaction part 51 and sample-adding process part 52 are connected as one.The sample-adding detachable set of process part 52
Be connected to outside the sample feeding pipe 513 of example reaction part 51, under socket state, the first roller 5223, the second roller 5225 be located at into
It is on sample pipe 513 and perpendicular with sample feeding pipe 513.Meanwhile, as shown in figure 5, dividing plate 5227 is additionally provided with support 5221, can
Control, separate the position on support member 521 of different sample feeding pipes 513.When in use, sample feeding pipe 513 can be placed horizontally at support
On part 521, and mutually separated by dividing plate 5227, it is to avoid sample feeding pipe 513 is intertwined and interferes.
As shown in Fig. 3,4,6,7, the central point of horizontal rail c is equal to or bigger to the vertical range of the top surface of support member 521
In the radius of the roller.Wherein, when the central point of horizontal rail c is slightly larger than roller to the vertical range of the top surface of support member 521
Radius when, its 0.05-0.1cm bigger than roller radius.Said structure ensures the first roller 5223, the second roller on track 5222
5225 can completely extrude blocking sample feeding pipe 513.In addition, 1/2- of the length of horizontal rail c for the horizontal length of support member 521
2/3。
By the following examples to the present invention in a kind of immunofluorescence analysis instrument be illustrated.
After user obtains a kind of immunofluorescence analysis instrument in the present invention, the sample-adding horizontal positioned of process part 52 is taken, beaten
Flow control member 522 is opened, many sample feeding pipes 513 of example reaction part 51 are put into, make support member 521 and flow control member
522 draw-in grooves are closed.The same terms are prepared into a batch of reagent strip again and is respectively put into each reagent strip in sample batch processing device 5
In the reagent strip chamber 5151 of room 515, and fixed by reagent strip fixed block 5152.Meanwhile, in the coding chamber 5161 of code room 516
Encoding strip 5162 and barrier film 5163 are inside provided with, barrier film 5163 is covered on encoding strip 5162, prevent batch from encoding and number
Coding comes off and can change at any time.
Then, promote the second roller 5225 on the second oblique orbit b, under be rolled to horizontal rail c, block many sample feeding pipes
513 outlets.Sample cell 512, sample is added to separately flow into many through sample cell 512 by adding mouth 511 respectively in above-mentioned sample
After sample feeding pipe 513, sample size is 120-150 μ l in many sample feeding pipes 513.Promote the first roller on the first oblique orbit a
5223, under be rolled to horizontal rail c, block the import of many sample feeding pipes 513, then promote the second roller 5225 on horizontal rail c, on
Rolled back the second oblique orbit b, and promotes the first roller 5223 to be rolled on horizontal rail c, and sample is extruded in making many sample feeding pipes 513
And through go out sample pipe 514 respectively simultaneously, equivalent is added drop-wise on the reagent strip in each reagent strip room 515.
After reaction 15 minutes, in immunofluorescence analysis instrument, by scan control circuit 1, control machinery transmission device 2 will
Laser diode is by spectroscope, the light beam of lens of excitation light path 4 in reagent strip and code room 516 in reagent strip room 515
Encoding strip 5162 carries out progressive scan transmitting fluorescence, and the wavelength of laser diode is 630nm.Transmitting fluorescence passes through fluoroscopic examination again
The optical filter of light path 6, lens, grating, produce fluorescence signal, amplify through integrated amplifier 7, and A/D adapters 8 are converted to telecommunications
Number, data processing is carried out in control unit 9, shown in liquid crystal display and touch-screen, discharge interference is input into by keyboard, USB device
Data and batch discrepancy adjustment data, and exported testing result by printer.After detection is finished, flow control member 522 is opened,
Example reaction part 51 is changed, new reagent strip is placed into, repeated the above steps, proceed sample batch detection.
Wherein, the detection range to fluorescence signal of immunofluorescence analysis instrument is AD values 0-10000.With sample concentration as horizontal
Coordinate, near-infrared fluorescent detector detects that fluorescence signal (average value) is ordinate, sets up standard curve.On standard curve,
With the rising of standard concentration, the fluorescence signal value for measuring also is increased, both proportional relations.
In sum, the present invention effectively overcomes various shortcoming of the prior art and has high industrial utilization.
The above-described embodiments merely illustrate the principles and effects of the present invention, not for the limitation present invention.It is any ripe
The personage for knowing this technology all can carry out modifications and changes under without prejudice to spirit and scope of the invention to above-described embodiment.Cause
This, those of ordinary skill in the art is complete with institute under technological thought without departing from disclosed spirit such as
Into all equivalent modifications or change, should be covered by claim of the invention.
Claims (10)
1. a kind of immunofluorescence analysis instrument, includes scan control circuit (1), mechanical driving device (2), the light source being sequentially connected
(3), excitation light path (4), fluorescence detection optical path (6), integrated amplifier (7), optical-electrical converter (8), control unit (9)；
Characterized in that, also including sample batch processing device (5), the sample batch processing device (5) includes following part：
Example reaction part (51), the example reaction part (51) is including being sequentially connected the adding mouth being integrated (511), sample
Pond (512), sample feeding pipe (513), go out sample pipe (514), reagent strip room (515), the reagent strip room (515) includes reagent strip chamber
(5151), wherein, sample cell (512) side is connected with the adding mouth (511), sample cell (512) opposite side
The one end with least two sample feeding pipes (513) is connected respectively, the other end of the sample feeding pipe (513) through go out sample pipe (514) with
Described reagent strip chamber (5151) one end is connected；
Sample-adding process part (52), sample-adding process part (52) includes support member (521), flow control member (522),
Wherein, the flow control member (522) includes hollow stent (5221) and roller, hollow stent (5221) both sides
Symmetrical a pair of tracks (5222) is respectively equipped with, the track (5222) includes the first oblique orbit (a), the second oblique orbit
B (), horizontal rail (c), first oblique orbit (a) is connected through horizontal rail (c) with second oblique orbit (b)；It is described
Roller includes the first roller (5223) and the second roller (5225), and the center of first roller (5223) is provided with the first rolling
Axle (5224), the center of second roller (5225) is provided with the second roller bearing (5226), the roller through roller bearing (5224,
5226) rolled with the pair of track (5222) and be connected；
The support member (521) and the leg detachable connection of the hollow stent (5221) directly over it；
It is described to be loaded process part (52) detachable sample feeding pipe (513) for being socketed on the example reaction part (51) outward,
Under socket state, first roller (5223), the second roller (5225) on the sample feeding pipe (513), and with it is described
Sample feeding pipe (513) is perpendicular.
2. immunofluorescence analysis instrument according to claim 1, it is characterised in that the height of the adding mouth (511) is higher than
The height of the sample cell (512)；Under the adding mouth (511) is near sample feeding pipe (513) side with the sample cell (512)
Cancave cambered surface.
3. immunofluorescence analysis instrument according to claim 1, it is characterised in that reagent strip chamber (5151) rear and front end
Be respectively provided on two sides with reagent strip fixed block (5152).
4. immunofluorescence analysis instrument according to claim 1, it is characterised in that the example reaction part (51) also includes
There is code room (516), the code room (516) includes coding chamber (5161) and encoding strip (5162), the encoding strip
(5162) it is covered in coding chamber (5161)；Barrier film (5163), the barrier film are additionally provided with the coding chamber (5161)
(5163) it is covered on the encoding strip (5162).
5. immunofluorescence analysis instrument according to claim 1, it is characterised in that the sample-adding process part (52) also includes
There is open and close controlling part (523), the open and close controlling part (523) includes contiguous block (5231), rotary shaft (5232), depression bar
(5233), horizontal block (5234), spring (5235), contiguous block (5231) side are connected with the support member (521), institute
State contiguous block (5231) opposite side to be connected with the horizontal block (5234), rotary shaft is additionally provided with inside the contiguous block (5234)
(5232), rotary shaft (5232) side is connected with the hollow stent (5221), rotary shaft (5232) opposite side
It is connected with the depression bar (5233), spring (5235) is additionally provided between the depression bar (5233) and the horizontal block (5234).
6. immunofluorescence analysis instrument according to claim 1, it is characterised in that be additionally provided with the hollow stent (5221)
Dividing plate (5227)；The caliber of the sample feeding pipe (513) goes out the caliber of sample pipe (514) more than described in.
7. immunofluorescence analysis instrument according to claim 1, it is characterised in that the light source (3) is laser diode, institute
The wavelength for stating laser diode is 600-650nm.
8. immunofluorescence analysis instrument according to claim 1, it is characterised in that the also external bounded of described control unit (9)
Face display module, data are uploaded and print module；The interface display module is liquid crystal display and touch-screen, the data upload and
Print module is keyboard, printer, USB device.
9. according to any described immunofluorescence analysis instrument of claim 1-8 immunofluorescence analysis field purposes.
10., according to the application method of any described immunofluorescence analysis instrument of claim 1-8, following steps are specifically included：
1) sample-adding process part horizontal positioned is taken, flow control member is opened, the sample feeding pipe of example reaction part is put into, closed
Afterwards, then by the reagent strip of batch processing be respectively put into reagent strip room, and promote the second oblique orbit on the second roller, under be rolled to water
Plate rail road, blocks sample feeding pipe outlet；
2) after adding adding mouth, sample to separately flow into sample feeding pipe through sample cell in sample, the first rolling on the first oblique orbit is promoted
Post, under be rolled to horizontal rail, block sample feeding pipe import；
3) the second roller in horizontal rail, the second oblique orbit of above rolling back are promoted, and promotes the first roller to be rolled in horizontal rail
It is dynamic, it is added drop-wise on reagent strip by sample extrusion in sample feeding pipe and through going out sample pipe and is reacted；
4) by scan control circuit control machinery transmission device by light source by excitation light path to reagent strip in reagent strip room and
Encoding strip carries out progressive scan transmitting fluorescence in code room, then produces fluorescence signal by fluorescence detection optical path, through integrated amplification
Device amplifies, and optical-electrical converter is converted to electric signal, data processing is carried out in control unit；
5) after detection is finished, flow control member is opened, changes example reaction part, place into new reagent strip, repeat above-mentioned step
Suddenly, sample batch detection is proceeded.
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| CN201510349446.XA CN104897635B (en) | 2015-06-19 | 2015-06-19 | Immunofluorescence analysis instrument |
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| CN201510349446.XA CN104897635B (en) | 2015-06-19 | 2015-06-19 | Immunofluorescence analysis instrument |
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| CN104897635B true CN104897635B (en) | 2017-06-16 |
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| CN113324964A (en) * | 2021-06-23 | 2021-08-31 | 上海八通生物科技股份有限公司 | Simple qualitative analysis device for fluorescence detection |
| CN116773841A (en) * | 2022-03-08 | 2023-09-19 | 上海萨迦生物科技有限公司 | Full-automatic immunity analyzer based on laser confocal technology and detection method thereof |
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| CA2842232C (en) * | 2005-03-10 | 2015-01-27 | Gen-Probe Incorporated | Systems and methods to perform assays for detecting or quantifying analytes within samples |
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