CN104897635A - Immunofluorescence analyzer - Google Patents

Immunofluorescence analyzer Download PDF

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Publication number
CN104897635A
CN104897635A CN201510349446.XA CN201510349446A CN104897635A CN 104897635 A CN104897635 A CN 104897635A CN 201510349446 A CN201510349446 A CN 201510349446A CN 104897635 A CN104897635 A CN 104897635A
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China
Prior art keywords
sample
roller
feeding pipe
reagent strip
immunofluorescence analysis
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CN201510349446.XA
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CN104897635B (en
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丁晓辉
王金鹏
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SHANGHAI CHEMTRON BIOTECH CO Ltd
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SHANGHAI CHEMTRON BIOTECH CO Ltd
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Abstract

The invention provides an immunofluorescence analyzer. The immunofluorescence analyzer comprises a scanning control circuit, a mechanical drive device, a light source, an exciting light path, a fluorescence detection light path, an integrated amplifier, a photoelectric converter, a control unit and a sample batch processing device. The scanning control circuit, the mechanical drive device, the light source, the exciting light path, the fluorescence detection light path, the integrated amplifier, the photoelectric converter and the control unit are sequentially connected with one another; the sample batch processing device comprises a sample reaction portion and a sample loading processing portion, and the sample loading processing portion comprises a support part, a flow control part and an opening control part. The immunofluorescence analyzer has the advantages that the immunofluorescence analyzer is of a structure based on coaxial confocal principles, is provided with the specific sample batch processing device and is matched with other corresponding instrument components, accordingly, a series of to-be-tested antibodies or antigen substances in large batches can be analyzed quickly, simultaneously and accurately, the immunofluorescence analyzer is particularly applicable to sites of sudden events and base courses and can be applied to clinical detection, and qualitative and quantitative bases can be provided for clinic treatment.

Description

Immunofluorescence analysis instrument
Technical field
The invention belongs to and analyze checkout equipment technical field, be specifically related to a kind of immunofluorescence analysis instrument.
Background technology
Immunofluorescence technique (Immunofluorescence assay, IFA) originated in early 1940s, by Coons etc. nineteen forty-one adopt first fluorescein labelled antibody check mouse tissue cut into slices in solubility pneumococcal polysaccharide antigen and succeed.Its measuring principle is: combine with antigen corresponding in sample (cell, tissue or the material etc. be separated) or antibody with fluorescently-labeled antibody or antigen, analyze with the technology of suitable detection fluorescence to it.Wherein, because fluorchrome not only can be combined with antibody globulin, for detect or locate various antigen, also can with other protein bound, for detect or location antibody.Therefore, the technology of carrying out antigen location with fluorescent material labelled antibody is called fluorescent antibody technics (fluorescent antibody technique), fluorescent antigen technology is claimed, these two kinds of method general name immunofluorescence techniques with the method for known fluorescent antigen label spike or inspection corresponding antibodies.But fluorescent antigen technology is seldom applied in real work, so fluorescent antibody technics is called immunofluorescence technique by people's custom.
Since 20 century 70s, on the basis of conventional fluorescent antibody technique, development establishes various fluorescence immunoassay (fluorescence immunoassay, FIA) can be used for the antigen in quantitative measurement body fluid or antibody, thus make immunofluorescence analysis technology enter a new developing stage.Fluorescence immunoassay is after antigen-antibody reaction, utilizes Instrument measuring fluorescence intensity and calculates the detection method of measured object concentration.Due to the specificity of FIA, rapidity and the accuracy that is decided to be at cellular level, the discovery of new fluorescent material and updating of labeling method, IFA is used widely in immunology, microbiology, pathology, oncology and clinical examination etc. are many.
At present, the analytical instrument that fluorescence immunoassay adopts is immunofluorescence analysis instrument, it is according to solid phase luminous reflectance chromogen reason, according to the label of luciferase assay reagent---fluorescent grain is under the laser excitation of near-infrared wavelength, inspire fluorescence signal, and the analytical instrument that the fluorescence signal intensity of kit detection line (T line) and sample concentration are followed certain corresponding relation and designed.
But, traditional immunofluorescence analysis instrument can only detect and reliability, stability and poor sensitivity a sample simultaneously, complicated operation, carry inconvenience, as can be seen here, also there is certain defect and have much room for improvement in traditional immunofluorescence analysis instrument, is necessary very much the standardization of immunofluorescence analysis instrument, quantification and robotization are further improved.
Summary of the invention
The shortcoming of prior art in view of the above, the object of the present invention is to provide a kind of immunofluorescence analysis instrument, can batch detection be carried out and the problem of the good immunofluorescence analysis instrument of the reliability of batch sample testing result, stability and sensitivity for solving in prior art to lack.
For achieving the above object and other relevant objects, the invention provides a kind of immunofluorescence analysis instrument, include the scan control circuit, mechanical driving device, light source, excitation light path, fluorescence detection optical path, integrated amplifier, photoelectric commutator, the control module that connect successively; Also include sample batch processing device, described sample batch processing device comprises with lower part:
Example reaction part, described example reaction part comprise connect as one successively adding mouth, sample cell, sample feeding pipe, go out sample pipe, reagent strip room, described reagent strip room includes reagent strip chamber, wherein, described sample cell side is connected with described adding mouth, described sample cell opposite side is connected with one end of at least two sample feeding pipes respectively, and the other end of described sample feeding pipe is connected with one end, described reagent strip chamber through going out sample pipe;
Application of sample processing section, described application of sample processing section includes support member, flow control member,
Wherein, described flow control member includes hollow stent and roller, described support both sides are respectively equipped with symmetrical pair of tracks, and described track includes the first oblique orbit, the second oblique orbit, horizontal rail, and described first oblique orbit is connected with described second oblique orbit through horizontal rail; Described roller comprises the first roller and the second roller, and the center of described first roller is provided with the first roller bearing, and the center of described second roller is provided with the second roller bearing, and described roller to roll with described pair of tracks through roller bearing and is connected;
Described support member is connected with the leg of the described hollow stent directly over it is detachable;
Outside the detachable sample feeding pipe being socketed on described example reaction part in described application of sample processing section, under socket state, described first roller, the second roller are positioned on described sample feeding pipe, and perpendicular with described sample feeding pipe.
Described example reaction part is single use parts.Use rear discardable.
Preferably, the height of described adding mouth is higher than the height of described sample cell.
Preferably, described adding mouth and described sample cell are lower cancave cambered surface near sample feeding pipe side.This shape and structure is conducive to ensureing that in sample feeding pipe, sample fills.
More preferably, the radian of described lower cancave cambered surface is 90-120 °.
Preferably, the both sides of rear and front end, described reagent strip chamber are respectively equipped with reagent strip fixed block.Described reagent strip fixed block can effective fixating reagent bar.
Preferably, described example reaction part also includes code room, and described code room includes coding chamber and encoding strip, and described encoding strip covers in coding chamber.Described encoding strip to include batch coding, an individual number encoder, and described batch of coding and a number encoder are arranged in order, described batch of coding and a number encoder can effectively identify sample batch and numbering number.
More preferably, be also provided with barrier film in described coding chamber, described barrier film covers on described encoding strip.Batch coding and a number encoder paste barrier film, prevents batch coding and the number encoder from coming off; When needed, can easily barrier film and encoding strip be taken off and change.
Most preferably, described barrier film is transparent and impermeable material.Described barrier film is transparent non-setting adhesive band.Protection encoding strip avoids reagent on reagent strip to stain.
Preferably, the caliber of described sample feeding pipe be greater than described in go out the caliber of sample pipe.Be conducive to by batch sample in sample feeding pipe respectively accurate quantitative analysis from going out on reagent strip that sample pipe is added dropwise to reagent strip chamber.
More preferably, the caliber going out sample pipe described in is the 1/3-1/4 of the caliber of described sample feeding pipe.
Preferably, described support member and the leg of the described hollow stent directly over it are that detachable draw-in groove is connected.
Preferably, described application of sample processing section also includes open and close controlling part.
More preferably, described open and close controlling part includes contiguous block, rotation axis, depression bar, horizontal block, spring, described contiguous block side is connected with described support member, described contiguous block opposite side is connected with described horizontal block, described contiguous block inside is also provided with rotation axis, described rotation axis side is connected with described support, and described rotation axis opposite side is connected with described depression bar, is also provided with spring between described depression bar and described horizontal block.Described open and close controlling part can control the opening and closing of support member and flow control member.
Preferably, described support is also provided with dividing plate.Described dividing plate can control, separate different sample feeding pipe position on support member.
Preferably, the central point of described horizontal rail to equal or slightly larger than the radius of described roller to the vertical range of support member end face.
More preferably, the central point of described horizontal rail is greater than the radius 0.05-0.1cm of described roller to the vertical range of support member end face.
Preferably, the length of described horizontal rail is the 1/2-2/3 of the horizontal length of described support member.
Described scan control circuit, mechanical driving device, light source, excitation light path, fluorescence detection optical path adopt the burnt light channel structure of coaxial copolymerization.Described scan control circuit adopts width modulation (PWM) principle, bipolar DC Chopper driving mode, the start-stop of Serial Control mechanical driving device, rotating speed and direction.Described mechanical driving device drives light source to line by line scan emitting fluorescence to the reagent strip room in sample batch processing device and code room under the driving of scan control circuit.
Preferably, described light source is laser diode, and the wavelength of described laser diode is 600-650nm.Within the scope of this, in reagent strip, in labeling pad, fluorescent marker has maximum excitation light.
More preferably, the wavelength of described laser diode is 630nm.
Preferably, described excitation light path includes the spectroscope, the lens that connect successively.
Preferably, described fluorescence detection optical path includes the optical filter, lens, the grating that connect successively.
Described integrated amplifier is amplified by the fluorescence signal inspired.
Described photoelectric commutator (i.e. A/D breakout box) is that fluorescence signal is converted to electric signal.
Described control module has the function of single-chip microcomputer and PC, the electric signal received can be carried out data processing, by making form specific funtcional relationship within the specific limits between testing sample concentration and absorbance and discharge interference, and output detections result after analyzing.Increase batch discrepancy adjustment system in described control module, effectively solve the difference between batch problem of testing sample.
Preferably, described control module is also circumscribed with interface display module, data upload and print module.
More preferably, described interface display module is liquid crystal display and touch-screen.
More preferably, described data upload and print module are keyboard, printer, USB device.
The sensing range of described immunofluorescence analysis instrument to fluorescence signal is AD value 0-10000.
The present invention still further provides the purposes of a kind of immunofluorescence analysis instrument in immunofluorescence analysis field.
The present invention also provides a kind of using method of immunofluorescence analysis instrument further, specifically comprises the following steps:
1) get application of sample processing section horizontal positioned, open flow control member, the sample feeding pipe of example reaction part is put into, after closed, then the reagent strip of batch processing is put into reagent strip room respectively, and promote the second roller on the second oblique orbit, under be rolled to horizontal rail, blocking sample feeding pipe outlet;
Preferably, described second roller is rolled by the second roller bearing in described second oblique orbit.
Preferably, described reagent strip is fixed in reagent strip chamber by reagent strip fixed block.
2) sample is added adding mouth, sample, after sample cell flows into sample feeding pipe respectively, promotes the first roller on the first oblique orbit, under be rolled to horizontal rail, blocking sample feeding pipe import;
Preferably, described first roller is rolled by the first roller bearing in described first oblique orbit.
3) promote the second roller in horizontal rail, the second oblique orbit of above rolling back, and promote the first roller and roll in horizontal rail, sample in sample feeding pipe is extruded and is added drop-wise on reagent strip reacts through going out sample pipe;
4) control mechanical driving device by scan control circuit light source to be lined by line scan emitting fluorescence to encoding strip in reagent strip in reagent strip room and code room by excitation light path, fluorescence signal is produced again by fluorescence detection optical path, amplify through integrated amplifier, photoelectric commutator is converted to electric signal, carries out data processing at control module;
5) after detecting, open flow control member, change example reaction part, then put into new reagent strip, repeat above-mentioned steps, proceed sample and criticize detection.
As mentioned above, a kind of immunofluorescence analysis instrument provided by the invention, by adopting the structure based on coaxial focusing principle altogether, specific sample batch processing device is provided, and the instrument component corresponding to other coordinates, can fast, a series of, the large batch test antibodies of analysis of accurate, qualitative, quantitative or antigenic substance.Particularly can to batch processing sample together accurate application of sample, the application of sample amount of batch processing sample is accurately consistent.In this device, sample is not easy to spill simultaneously, thus avoids the metrical error because sample loss causes.Visible, this immunofluorescence analysis instrument sample collection and process in testing process simply, can carry out effective batch processing, batch detection to sample, easy to detect, cost is low, sensitivity and precision high, specificity is good, simultaneously, detecting data can deposit readable, automaticity is high, can obtain result fast and accurately, is applicable to very much accident scene and basic unit's use, and may be used in clinical detection, for clinical treatment provides qualitative, quantitative foundation.
Accompanying drawing explanation
Fig. 1 is shown as the analysis process figure of a kind of immunofluorescence analysis instrument of the present invention.
Fig. 2 is shown as the control module annexation schematic diagram of a kind of immunofluorescence analysis instrument of the present invention.
Fig. 3 is shown as the planar structure schematic diagram of the sample batch processing device of a kind of immunofluorescence analysis instrument of the present invention.
Fig. 4 is shown as the planar structure schematic diagram of the example reaction part of a kind of immunofluorescence analysis instrument of the present invention.
Fig. 5 is shown as the planar structure schematic diagram of the application of sample processing section of a kind of immunofluorescence analysis instrument of the present invention.
Fig. 6 is shown as the side structure schematic diagram of the sample batch processing device of a kind of immunofluorescence analysis instrument of the present invention.
Fig. 7 is shown as the cross-sectional structure schematic diagram of the application of sample processing section of a kind of immunofluorescence analysis instrument of the present invention.
Reference numeral
1 scan control circuit
2 mechanical driving devices
3 light sources
4 excitation light paths
5 sample batch processing devices
51 example reaction parts
511 adding mouths
512 sample cells
513 sample feeding pipes
514 go out sample pipe
515 reagent strip rooms
5151 reagent strip chambeies
5152 reagent strip fixed blocks
516 code rooms
5161 coding chambeies
5162 encoding strips
5163 barrier films
52 application of sample processing sections
521 support members
522 flow control members
5221 supports
5222 tracks
A first oblique orbit
B second oblique orbit
C horizontal rail
5223 first rollers
5224 first roller bearings
5225 second rollers
5226 second roller bearings
5227 dividing plates
523 open and close controlling parts
5231 contiguous blocks
5232 rotation axiss
5233 depression bars
5234 horizontal block
5235 springs
6 fluorescence detection optical paths
7 integrated amplifiers
8 photoelectric commutators
9 control modules
Embodiment
Below by way of specific instantiation, embodiments of the present invention are described, those skilled in the art the content disclosed by this instructions can understand other advantages of the present invention and effect easily.The present invention can also be implemented or be applied by embodiments different in addition, and the every details in this instructions also can based on different viewpoints and application, carries out various modification or change not deviating under spirit of the present invention.
Before further describing the specific embodiment of the invention, should be understood that protection scope of the present invention is not limited to following specific specific embodiments; It is also understood that the term used in the embodiment of the present invention is to describe specific specific embodiments, instead of in order to limit the scope of the invention; In instructions of the present invention and claims, unless explicitly pointed out in addition in literary composition, singulative " ", " one " and " this " comprise plural form.
When embodiment provides numerical range, should be understood that except non-invention is otherwise noted, between two end points of each numerical range and two end points, any one numerical value all can be selected.Unless otherwise defined, all technology used in the present invention are identical with the meaning that those skilled in the art of the present technique understand usually with scientific terminology.Except the concrete grammar used in embodiment, equipment, material, according to those skilled in the art to the grasp of prior art and record of the present invention, any method of prior art that is similar with the method described in the embodiment of the present invention, equipment, material or that be equal to, equipment and material can also be used to realize the present invention.
Meanwhile, Fig. 1 to Fig. 7 is referred to.Notice, structure, ratio, size etc. that this instructions institute accompanying drawings illustrates, content all only in order to coordinate instructions to disclose, understand for person skilled in the art scholar and read, and be not used to limit the enforceable qualifications of the present invention, therefore the not technical essential meaning of tool, the adjustment of the modification of any structure, the change of proportionate relationship or size, do not affecting under effect that the present invention can produce and the object that can reach, still all should drop on disclosed technology contents and obtain in the scope that can contain.Simultaneously, quote in this instructions as " on ", D score, "left", "right", " centre " and " one " etc. term, also only for ease of understanding of describing, and be not used to limit the enforceable scope of the present invention, the change of its relativeness or adjustment, under changing technology contents without essence, when being also considered as the enforceable category of the present invention.
As shown in Figure 1-2, the invention provides a kind of immunofluorescence analysis instrument, include the scan control circuit 1, mechanical driving device 2, light source 3, excitation light path 4, fluorescence detection optical path 6, integrated amplifier 7, photoelectric commutator 8, the control module 9 that connect successively, also include sample batch processing device 5.
Scan control circuit 1, mechanical driving device 2, light source 3, excitation light path 4, fluorescence detection optical path 6, integrated amplifier 7, photoelectric commutator 8, control module 9 are prior art.Concrete annexation is as shown in Figure 1.
Wherein, light source 3 is laser diode, and the wavelength of laser diode is 600-650nm, is preferably 630nm.In this wavelength coverage, there is maximum excitation light to fluorescent marker in labeling pad in reagent strip.Excitation light path 4 includes the spectroscope, the lens that connect successively, can be effectively irradiated to by exciting light in the reagent strip room 515 in sample batch processing device 5 in reagent strip and code room 516 on encoding strip 5162.Fluorescence detection optical path 6 includes the optical filter, lens, the grating that connect successively, fluorescence after irradiation can be produced fluorescence signal, and be transferred in integrated amplifier 7.The fluorescence signal received amplifies by integrated amplifier 7, and is that fluorescence signal is converted to electric signal by photoelectric commutator (i.e. A/D breakout box), Input Control Element 9.
As shown in Figure 2, control module 9 is also circumscribed with interface display module, data upload and print module.Interface display module is liquid crystal display and touch-screen, and data upload and print module are keyboard, printer, USB device.Control module 9 has the function of single-chip microcomputer and PC, the electric signal received can be carried out data processing, by making form specific funtcional relationship within the specific limits between testing sample concentration and absorbance and discharge interference, and output detections result after analyzing.
As shown in Figure 1, sample batch processing device 5 includes example reaction part 51 and application of sample processing section 52.Example reaction part 51 and application of sample processing section 52 are split-type structural.
As shown in Fig. 3,4,6, example reaction part 51 comprise connect as one successively adding mouth 511, sample cell 512, sample feeding pipe 513, go out sample pipe 514, reagent strip room 515, reagent strip room 515 includes reagent strip chamber 5151, wherein, sample cell 512 side is connected with adding mouth 511, sample cell 512 opposite side is connected with one end of at least two sample feeding pipes 513 respectively, and the other end of sample feeding pipe 513 is connected with one end, reagent strip chamber 5151 through going out sample pipe 514.Example reaction part 51 is single use parts, uses rear discardable.
Wherein, as shown in Figure 3,4, the both sides of rear and front end, reagent strip chamber 5151 are respectively equipped with reagent strip fixed block 5152, can effective fixating reagent bar.As shown in Fig. 3,4,6, example reaction part 51 also includes code room 516, and code room 516 includes coding chamber 5161 and encoding strip 5162, and encoding strip 5162 covers in coding chamber 5161; Coding is also provided with barrier film 5163 in chamber 5161, and barrier film 5163 covers on encoding strip 5162.Encoding strip 5162 to include batch coding, an individual number encoder, and described batch of coding and a number encoder are arranged in order, described batch of coding and a number encoder can effectively identify sample batch and numbering number.Batch coding and a number encoder paste barrier film 5163, prevents batch coding and the number encoder from coming off; When needed, can easily barrier film 5163 and encoding strip 5162 be taken off and change.Barrier film 5163 is transparent and impermeable material, is specially transparent non-setting adhesive band.
As shown in Figure 6, the height of adding mouth 511 is higher than the height of sample cell 512; Adding mouth 511 and sample cell 512 are lower cancave cambered surface near sample feeding pipe 513 side, and the radian of lower cancave cambered surface is 90-120 °.When this shape and structure is conducive to ensureing application of sample, sample flows into sample cell 512 from adding mouth 511, then flows into sample feeding pipe 513, can ensure that in sample feeding pipe 513, sample fills.
As shown in Fig. 3,4,6, the caliber of sample feeding pipe 513 is greater than the caliber of sample pipe 514.Because the caliber going out sample pipe 514 is little, be conducive to by batch sample in sample feeding pipe 513 respectively accurate quantitative analysis from going out on reagent strip that sample pipe 514 is added dropwise to reagent strip chamber 5151.The caliber specifically going out sample pipe 514 is the 1/3-1/4 of the caliber of sample feeding pipe 513.
As shown in Fig. 5,6,7, application of sample processing section 52 includes support member 521, flow control member 522.
Wherein, as shown in Fig. 3,5,6,7, flow control member 522 includes hollow stent 5221 and roller, support 5221 both sides are respectively equipped with symmetrical pair of tracks 5222, track 5222 includes the first oblique orbit a, the second oblique orbit b, horizontal rail c, and the first oblique orbit a is connected with the second oblique orbit b through horizontal rail c; The center that the center that roller comprises the first roller 5223 and the second roller 5225, first roller 5223 is provided with the first roller bearing 5224, second roller 5225 is provided with the second roller bearing 5226, and roller to roll with pair of tracks 5222 through roller bearing 5224,5226 and is connected.
Rolled to horizontal rail c from the first oblique orbit a by the first roller bearing 5224 of the center of the first roller 5223, effectively can block the import of multiple sample feeding pipe 513, and sample in multiple sample feeding pipe 513 is pushed be exported through going out on the reagent strip that sample pipe 514 is added dropwise in multiple reagent strip chamber 5151 respectively by sample feeding pipe 513.Meanwhile, rolled from the second oblique orbit b to horizontal rail c by the second roller bearing 5226 of the center of the second roller 5225, effectively can block the outlet of multiple sample feeding pipe 513, play the effect of valve.Sample in multiple sample feeding pipe 513 can be added drop-wise on reagent strip by this structure simultaneously simultaneously, and controlled by the first roller 5223 and the dripping quantity of the second roller 5225 to multiple sample, ensure that sample size is consistent from the reagent strip of sample feeding pipe 513 through going out sample pipe 514 and being added dropwise to multiple reagent strip chamber 5151 respectively.
As shown in Figure 6,7, support member 521 is connected with the leg of the hollow stent 5221 directly over it is detachable.Both detachable connections are preferably detachable draw-in groove and connect.
In addition, as shown in Figure 7, application of sample processing section 52 also includes open and close controlling part 523, open and close controlling part 523 includes contiguous block 5231, rotation axis 5232, depression bar 5233, horizontal block 5234, spring 5235, contiguous block 5231 side is connected with support member 521, contiguous block 5231 opposite side is connected with horizontal block 5234, contiguous block 5234 inside is also provided with rotation axis 5232, rotation axis 5232 side is connected with support 5221, rotation axis 5232 opposite side is connected with depression bar 5233, is also provided with spring 5235 between depression bar 5233 and horizontal block 5234.Open and close controlling part 523 effectively can control support member 521 and close with the opening and closing of flow control member 522.Operationally, by pressing down-pressed pole 5233, under rotation axis 5232 acts on, the spring 5235 between compression depression bar 5233 and horizontal block 5234, makes lifting of flow control member 522 medium-height trestle 5221; Decontrol depression bar 5233, the spring 5235 between depression bar 5233 and horizontal block 5234 restores to the original state, and impels depression bar 5233 to lift, and makes the rotation axis 5232 be connected with depression bar 5233 drive support 5221 to close with support member 521.
As Figure 1-3, generally, example reaction part 51 and application of sample processing section 52 are split-type structural.When performing a test, example reaction part 51 and application of sample processing section 52 connect as one.Application of sample processing section 52 is detachable to be socketed on outside the sample feeding pipe 513 of example reaction part 51, and under socket state, the first roller 5223, second roller 5225 is positioned on sample feeding pipe 513, and perpendicular with sample feeding pipe 513.Meanwhile, as shown in Figure 5, support 5221 is also provided with dividing plate 5227, can controls, separate different sample feeding pipe 513 position on support member 521.In use, sample feeding pipe 513 can be placed horizontally on support member 521, and be separated by dividing plate 5227 phase, avoid sample feeding pipe 513 to be intertwined and mutually disturb.
As shown in Fig. 3,4,6,7, the central point of horizontal rail c to equal or slightly larger than the radius of described roller to the vertical range of support member 521 end face.Wherein, when the central point of horizontal rail c is to radius slightly larger than roller of the vertical range of support member 521 end face, its 0.05-0.1cm larger than roller radius.Said structure ensures that on track 5222, first roller 5223, second roller 5225 can extrude blocking sample feeding pipe 513 completely.In addition, the length of horizontal rail c is the 1/2-2/3 of the horizontal length of support member 521.
By the following examples a kind of immunofluorescence analysis instrument in the present invention is set forth.
User gets application of sample processing section 52 horizontal positioned, opens flow control member 522 after obtaining a kind of immunofluorescence analysis instrument in the present invention, is put into by the many sample feeding pipes 513 of example reaction part 51, and support member 521 is closed with flow control member 522 draw-in groove.The reagent strip again being prepared by the same terms one batch puts into the reagent strip chamber 5151 of each reagent strip room 515 of sample batch processing device 5 respectively, and is fixed by reagent strip fixed block 5152.Meanwhile, in the coding chamber 5161 of code room 516, be provided with encoding strip 5162 and barrier film 5163, barrier film 5163 covers on encoding strip 5162, prevents batch coding and the number encoder from coming off and can change at any time.
Then, promote the second roller 5225 on the second oblique orbit b, under be rolled to horizontal rail c, block many sample feeding pipes 513 and export.Above-mentioned sample is added sample cell 512 respectively by adding mouth 511, and sample flows into after many sample feeding pipes 513 through sample cell 512 respectively, and in many sample feeding pipes 513, sample size is 120-150 μ l.Promote the first roller 5223 on the first oblique orbit a, under be rolled to horizontal rail c, block the import of many sample feeding pipes 513, promote the second roller 5225 on horizontal rail c again, on to roll back the second oblique orbit b, and promote the first roller 5223 and roll on horizontal rail c, sample in many sample feeding pipes 513 is extruded and through go out sample pipe 514 respectively simultaneously, equivalent is added drop-wise on the reagent strip in each reagent strip room 515.
React after 15 minutes, in immunofluorescence analysis instrument, by scan control circuit 1, control mechanical driving device 2 to be lined by line scan emitting fluorescence to encoding strip 5162 in reagent strip in reagent strip room 515 and code room 516 by the spectroscope of excitation light path 4, the light beam of lens by laser diode, the wavelength of laser diode is 630nm.Emitting fluorescence is again by optical filter, lens, the grating of fluorescence detection optical path 6, produce fluorescence signal, amplify through integrated amplifier 7, A/D breakout box 8 is converted to electric signal, data processing is carried out at control module 9, in liquid crystal display and touch-screen display, discharge interfering data and batch discrepancy adjustment data by keyboard, USB device input, and by printer, testing result is exported.After detection, open flow control member 522, change example reaction part 51, then put into new reagent strip, repeat above-mentioned steps, proceed sample and criticize detection.
Wherein, immunofluorescence analysis instrument is AD value 0-10000 to the sensing range of fluorescence signal.Take sample concentration as horizontal ordinate, it is ordinate that near-infrared fluorescent detector detects fluorescence signal (mean value), Criterion curve.On typical curve, along with the rising of standard concentration, the fluorescence signal value recorded also increases, both proportional relations.
In sum, the present invention effectively overcomes various shortcoming of the prior art and tool high industrial utilization.
Above-described embodiment is illustrative principle of the present invention and effect thereof only, but not for limiting the present invention.Any person skilled in the art scholar all without prejudice under spirit of the present invention and category, can modify above-described embodiment or changes.Therefore, such as have in art usually know the knowledgeable do not depart from complete under disclosed spirit and technological thought all equivalence modify or change, must be contained by claim of the present invention.

Claims (10)

1. an immunofluorescence analysis instrument, include connect successively scan control circuit (1), mechanical driving device (2), light source (3),
Excitation light path (4), fluorescence detection optical path (6), integrated amplifier (7), photoelectric commutator (8), control module (9);
It is characterized in that, also comprise sample batch processing device (5), described sample batch processing device (5) comprises with lower part:
Example reaction part (51), described example reaction part (51) comprises the adding mouth (511) connected as one successively, sample cell (512), sample feeding pipe (513), go out sample pipe (514), reagent strip room (515), described reagent strip room (515) includes reagent strip chamber (5151), wherein, described sample cell (512) side is connected with described adding mouth (511), described sample cell (512) opposite side is connected with one end of at least two sample feeding pipes (513) respectively, the other end of described sample feeding pipe (513) is connected with described reagent strip chamber (5151) one end through going out sample pipe (514),
Application of sample processing section (52), described application of sample processing section (52) includes support member (521), flow control member (522),
Wherein, described flow control member (522) includes hollow stent (5221) and roller, described support (5221) both sides are respectively equipped with symmetrical pair of tracks (5222), described track (5222) includes the first oblique orbit (a), the second oblique orbit (b), horizontal rail (c), and described first oblique orbit (a) is connected with described second oblique orbit (b) through horizontal rail (c); Described roller comprises the first roller (5223) and the second roller (5225), the center of described first roller (5223) is provided with the first roller bearing (5224), the center of described second roller (5225) is provided with the second roller bearing (5226), and described roller to roll with described pair of tracks (5222) through roller bearing (5224,5226) and is connected;
Described support member (521) is connected with the leg of the described hollow stent (5221) directly over it is detachable;
Described application of sample processing section (52) is detachable is socketed on the sample feeding pipe (513) of described example reaction part (51) outward, under socket state, described first roller (5223), the second roller (5225) are positioned on described sample feeding pipe (513), and perpendicular with described sample feeding pipe (513).
2. immunofluorescence analysis instrument according to claim 1, is characterized in that, the height of described adding mouth (511) is higher than the height of described sample cell (512); Described adding mouth (511) and described sample cell (512) are lower cancave cambered surface near sample feeding pipe (513) side.
3. immunofluorescence analysis instrument according to claim 1, is characterized in that, the both sides of described reagent strip chamber (5151) rear and front end are respectively equipped with reagent strip fixed block (5152).
4. immunofluorescence analysis instrument according to claim 1, it is characterized in that, described example reaction part (51) also includes code room (516), described code room (516) includes coding chamber (5161) and encoding strip (5162), and described encoding strip (5162) covers in coding chamber (5161); Also be provided with barrier film (5163) in described coding chamber (5161), described barrier film (5163) covers on described encoding strip (5162).
5. immunofluorescence analysis instrument according to claim 1, it is characterized in that, described application of sample processing section (52) also includes open and close controlling part (523), described open and close controlling part (523) includes contiguous block (5231), rotation axis (5232), depression bar (5233), horizontal block (5234), spring (5235), described contiguous block (5231) side is connected with described support member (521), described contiguous block (5231) opposite side is connected with described horizontal block (5234), described contiguous block (5234) inside is also provided with rotation axis (5232), described rotation axis (5232) side is connected with described support (5221), described rotation axis (5232) opposite side is connected with described depression bar (5233), spring (5235) is also provided with between described depression bar (5233) and described horizontal block (5234).
6. immunofluorescence analysis instrument according to claim 1, is characterized in that, described support (5221) is also provided with dividing plate (5227); The caliber of sample pipe (514) is gone out described in the caliber of described sample feeding pipe (513) is greater than.
7. immunofluorescence analysis instrument according to claim 1, is characterized in that, described light source (3) is laser diode, and the wavelength of described laser diode is 600-650nm.
8. immunofluorescence analysis instrument according to claim 1, is characterized in that, described control module (9) is also circumscribed with interface display module, data upload and print module; Described interface display module is liquid crystal display and touch-screen, and described data upload and print module are keyboard, printer, USB device.
9. according to the arbitrary described purposes of immunofluorescence analysis instrument in immunofluorescence analysis field of claim 1-8.
10., according to the using method of the arbitrary described immunofluorescence analysis instrument of claim 1-8, specifically comprise the following steps:
1) get application of sample processing section horizontal positioned, open flow control member, the sample feeding pipe of example reaction part is put into, after closed, then the reagent strip of batch processing is put into reagent strip room respectively, and promote the second roller on the second oblique orbit, under be rolled to horizontal rail, blocking sample feeding pipe outlet;
2) sample is added adding mouth, sample, after sample cell flows into sample feeding pipe respectively, promotes the first roller on the first oblique orbit, under be rolled to horizontal rail, blocking sample feeding pipe import;
3) promote the second roller in horizontal rail, the second oblique orbit of above rolling back, and promote the first roller and roll in horizontal rail, sample in sample feeding pipe is extruded and is added drop-wise on reagent strip reacts through going out sample pipe;
4) control mechanical driving device by scan control circuit light source to be lined by line scan emitting fluorescence to encoding strip in reagent strip in reagent strip room and code room by excitation light path, fluorescence signal is produced again by fluorescence detection optical path, amplify through integrated amplifier, photoelectric commutator is converted to electric signal, carries out data processing at control module;
5) after detecting, open flow control member, change example reaction part, then put into new reagent strip, repeat above-mentioned steps, proceed sample and criticize detection.
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