CN104892718A - Preparation method for extracting saponin powder by using camellia seed cake - Google Patents
Preparation method for extracting saponin powder by using camellia seed cake Download PDFInfo
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- CN104892718A CN104892718A CN201410085120.6A CN201410085120A CN104892718A CN 104892718 A CN104892718 A CN 104892718A CN 201410085120 A CN201410085120 A CN 201410085120A CN 104892718 A CN104892718 A CN 104892718A
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- tea
- saponin
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- seed pancake
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J63/00—Steroids in which the cyclopenta(a)hydrophenanthrene skeleton has been modified by expansion of only one ring by one or two atoms
- C07J63/008—Expansion of ring D by one atom, e.g. D homo steroids
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/20—Carbocyclic rings
- C07H15/24—Condensed ring systems having three or more rings
- C07H15/256—Polyterpene radicals
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a preparation method for extracting a saponin powder by using a camellia seed cake. The preparation method comprises the steps: drying and crushing the camellia seed cake, adding into a proper amount of a methanol solution according to the material-liquid ratio of 1:(4-8), leaching for 1-2 h at the temperature of 55 DEG C-75 DEG C, concentrating, and precipitating to obtain the product. Compared with a conventional extraction method, the method has the advantages of short extraction time, high extraction rate of the saponin powder, high purity, comprehensive utilization of the camellia seed cake resource and the like. The extraction rate of the saponin powder can reach 93.5% or more, the purity of the saponin powder is 80.7% or more, and 28 kg of proteins can be extracted from per ton of the camellia seed cake.
Description
Technical field
The present invention relates to technical field of biological extraction, particularly a kind of tea-seed pancake extracts the preparation method of saponin powder.
Background technology
Oil tea is the important oilseed plant of one of south China knob, and China is that oil tea distribution is the widest in the world, and the country that kind is maximum, produce tea seed about 54.98 ten thousand tons per year, the annual output of oil tea industry byproduct---tea-seed pancake is about 39.71 ten thousand tons.Be rich in saponin powder, fat, starch, tea soap rope, robust fibre in tea-seed pancake, in tea-seed pancake, saponin powder content is about 3%.Tea saponin is the natural nonionogenic tenside of excellent property, and there is haemolysis, impermeabilisation, only ancient term for country school, only phlegm, anti-inflammatory, cough-relieving, eliminate the phlegm, ease pain, anticancer, sterilization, multiple physiologically active and the physiological function such as desinsection.It is widely used: be industrially used as grease-removing agent, emulsifying agent and anti-friction composition etc.; Be used for making washing composition and shampoo, have the advantage that other synthetic is incomparable; Pharmaceutically can be used as the medicine for the treatment of diarrhoea, oedema and old trachitis; Agriculturally can be used as desinsection, sterilant and inert ingredient.China abounds with oil tea, and in tea-seed pancake cypress, the content of tea saponin is higher, if can make full use of tea-seed pancake to extract tea saponin and comprehensive utilization, will produce very large Social benefit and economic benefit.At present, the basic skills of industrial extraction tea saponin is water seaoning and organic solvent extractionprocess.Water seaoning technique is simple, and cost is low, but product is many in pulpous state, and second-rate, purity is 10-40%.Organic solvent extractionprocess gained solid phase prod purity bunch 75%, but its complex process, investment is large, and cost is higher.
Summary of the invention
The technical problem to be solved in the present invention is: provide a kind of tea-seed pancake to extract the preparation method of saponin powder, this preparation method's production technique simplifies, and cost is lower, and the saponin powder purity after extraction is high.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
Tea-seed pancake extracts a preparation method for saponin powder, the steps include:
A, by tea-seed pancake dry 1-2 hour at 40 DEG C of-60 DEG C of temperature, to pulverize, cross 10-22 mesh sieve, obtain tea-seed pancake powder;
B, in tea-seed pancake powder, add in the ratio of 1: 4-8 the methyl alcohol that concentration is 55%-75%; Lixiviate 1-2 hour at 55 DEG C of-75 DEG C of temperature;
C, filtered while hot, concentrated at 55 DEG C of-75 DEG C of temperature, then be evaporated to thick, stop heating, obtain pulpous state tea saponin;
D, in pulpous state tea saponin, add 72# precipitation agent in the ratio of 1: 0.6-1, fully stir, place after 1-3 hour, precipitation separation agent, obtains saponin powder;
E, by the saponin powder obtained dry be thick product.
F, by above-mentioned steps repeat extract 3-5 time, highly purified saponin powder can be obtained.
Described tea-seed pancake drying temperature is 50 DEG C, 1.5 hours time of drying.
Described tea-seed pancake powder and the solid-liquid ratio of methanol solution are 1: 6.
The concentration of described methanol solution is 65%, and at temperature 65 DEG C, lixiviate is 1.5 hours.
Described thickening temperature is 65 DEG C.
The ratio of described pulpous state tea saponin and 72# precipitation agent is 1: 0.8, and after stirring, storage period is 1.5 hours.
Describedly repeat extraction 4 times by above-mentioned steps.
The invention has the beneficial effects as follows: the inventive method average recoveries 93.5%, product purity is 80.7%, and tea-seed pancake per ton can extract 28 kilograms, protein.This technique, compared with organic solvent extractionprocess, does not need the skimming processes before lixiviate, also can reach the object of degreasing.The use of 72# precipitation agent, solve the difficult problem such as purifying and drying in production preferably, extraction yield is high, and good product quality, purity are high.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
Embodiment one
A, by tea-seed pancake at 50 DEG C of temperature dry 1.5 hours, pulverize, cross 16 mesh sieves, obtain tea-seed pancake powder;
B, in tea-seed pancake powder, add in the ratio of 1: 6 methyl alcohol that concentration is 65%; Lixiviate 1.5 hours at 65 DEG C of temperature;
C, filtered while hot, concentrated at 65 DEG C of temperature, then be evaporated to thick, stop heating, obtain pulpous state tea saponin;
D, in pulpous state tea saponin, add 72# precipitation agent in the ratio of 1: 0.8, fully stir, place after 1.5 hours, precipitation separation agent, obtains saponin powder;
E, by the saponin powder obtained dry be thick product.
F, repeat extraction 4 times by above-mentioned steps, highly purified saponin powder can be obtained.
Embodiment two
A, by tea-seed pancake at 40 DEG C of temperature dry 1 hour, pulverize, cross 10 mesh sieves, obtain tea-seed pancake powder;
B, in tea-seed pancake powder, add in the ratio of 1: 4 methyl alcohol that concentration is 55%; Lixiviate 1 hour at 55 DEG C of temperature;
C, filtered while hot, concentrated at 55 DEG C of temperature, then be evaporated to thick, stop heating, obtain pulpous state tea saponin;
D, in pulpous state tea saponin, add 72# precipitation agent in the ratio of 1: 0.6, fully stir, place after 1 hour, precipitation separation agent, obtains saponin powder;
E, by the saponin powder obtained dry be thick product.
F, repeat extraction 3 times by above-mentioned steps, highly purified saponin powder can be obtained.
Embodiment three
A, by tea-seed pancake at 60 DEG C of temperature dry 2 hours, pulverize, cross 22 mesh sieves, obtain tea-seed pancake powder;
B, in tea-seed pancake powder, add in the ratio of 1: 8 methyl alcohol that concentration is 75%; Lixiviate 2 hours at 75 DEG C of temperature;
C, filtered while hot, concentrated at 75 DEG C of temperature, then be evaporated to thick, stop heating, obtain pulpous state tea saponin;
D, in pulpous state tea saponin, add 72# precipitation agent in the ratio of 1: 1, fully stir, place after 2 hours, precipitation separation agent, obtains saponin powder;
E, by the saponin powder obtained dry be thick product.
F, repeat extraction 5 times by above-mentioned steps, highly purified saponin powder can be obtained.
Claims (7)
1. extract a preparation method for saponin powder with tea-seed pancake, the steps include:
A, by tea-seed pancake dry 1-2 hour at 40 DEG C of-60 DEG C of temperature, to pulverize, cross 10-22 mesh sieve, obtain tea-seed pancake powder;
B, in tea-seed pancake powder, add in the ratio of 1: 4-8 the methyl alcohol that concentration is 55%-75%; Lixiviate 1-2 hour at 55 DEG C of-75 DEG C of temperature;
C, filtered while hot, concentrated at 55 DEG C of-75 DEG C of temperature, then be evaporated to thick, stop heating, obtain pulpous state tea saponin;
D, in pulpous state tea saponin, add 72# precipitation agent in the ratio of 1: 0.6-1, fully stir, place after 1-3 hour, precipitation separation agent, obtains saponin powder;
E, by the saponin powder obtained dry be thick product.
F, by above-mentioned steps repeat extract 3-5 time, highly purified saponin powder can be obtained.
2. a kind of tea-seed pancake according to claim 1 extracts the preparation method of saponin powder, it is characterized in that: described tea-seed pancake drying temperature is 50 DEG C, 1.5 hours time of drying.
3. a kind of tea-seed pancake according to claim 1 extracts the preparation method of saponin powder, it is characterized in that: described tea-seed pancake powder and the solid-liquid ratio of methanol solution are 1: 6.
4. a kind of tea-seed pancake according to claim 1 extracts the preparation method of saponin powder, and it is characterized in that: the concentration of described methanol solution is 65%, at temperature 65 DEG C, lixiviate is 1.5 hours.
5. a kind of tea-seed pancake according to claim 1 extracts the preparation method of saponin powder, it is characterized in that: described thickening temperature is 65 DEG C.
6. a kind of tea-seed pancake according to claim 1 extracts the preparation method of saponin powder, it is characterized in that: the ratio of described pulpous state tea saponin and 72# precipitation agent is 1: 0.8, and after stirring, storage period is 1.5 hours.
7. a kind of tea-seed pancake according to claim 1 extracts the preparation method of saponin powder, it is characterized in that: describedly repeat extraction 4 times by above-mentioned steps.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410085120.6A CN104892718A (en) | 2014-03-05 | 2014-03-05 | Preparation method for extracting saponin powder by using camellia seed cake |
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| CN201410085120.6A CN104892718A (en) | 2014-03-05 | 2014-03-05 | Preparation method for extracting saponin powder by using camellia seed cake |
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| CN201410085120.6A Pending CN104892718A (en) | 2014-03-05 | 2014-03-05 | Preparation method for extracting saponin powder by using camellia seed cake |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111449090A (en) * | 2020-04-13 | 2020-07-28 | 湖南润农生态茶油有限公司 | Preparation method of tea seed cake extract |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1224022A (en) * | 1998-04-17 | 1999-07-28 | 重庆市协瑞科贸有限公司 | Process for extracting theasaponin |
| CN101830948A (en) * | 2010-05-11 | 2010-09-15 | 武汉工业学院 | Method for preparing tea saponin by using tea seed degreased dreg |
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2014
- 2014-03-05 CN CN201410085120.6A patent/CN104892718A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1224022A (en) * | 1998-04-17 | 1999-07-28 | 重庆市协瑞科贸有限公司 | Process for extracting theasaponin |
| CN101830948A (en) * | 2010-05-11 | 2010-09-15 | 武汉工业学院 | Method for preparing tea saponin by using tea seed degreased dreg |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111449090A (en) * | 2020-04-13 | 2020-07-28 | 湖南润农生态茶油有限公司 | Preparation method of tea seed cake extract |
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Application publication date: 20150909 |