CN104886605B - A kind of extraction and refinement method of high anti-oxidation dietary fiber - Google Patents
A kind of extraction and refinement method of high anti-oxidation dietary fiber Download PDFInfo
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- CN104886605B CN104886605B CN201510245959.6A CN201510245959A CN104886605B CN 104886605 B CN104886605 B CN 104886605B CN 201510245959 A CN201510245959 A CN 201510245959A CN 104886605 B CN104886605 B CN 104886605B
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- enzymolysis
- dietary fiber
- complex enzyme
- red bayberry
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- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 18
- 230000003064 anti-oxidating effect Effects 0.000 title claims abstract description 5
- 238000000605 extraction Methods 0.000 title claims abstract description 4
- 244000132436 Myrica rubra Species 0.000 claims abstract description 26
- 235000007270 Gaultheria hispida Nutrition 0.000 claims abstract description 24
- 235000009134 Myrica cerifera Nutrition 0.000 claims abstract description 24
- 244000269152 Myrica pensylvanica Species 0.000 claims abstract description 24
- 235000012851 Myrica pensylvanica Nutrition 0.000 claims abstract description 24
- 102000004190 Enzymes Human genes 0.000 claims abstract description 20
- 108090000790 Enzymes Proteins 0.000 claims abstract description 20
- 229940088598 enzyme Drugs 0.000 claims abstract description 20
- 239000000843 powder Substances 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000001035 drying Methods 0.000 claims abstract description 10
- 238000001914 filtration Methods 0.000 claims abstract description 10
- 230000007062 hydrolysis Effects 0.000 claims abstract description 10
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 10
- 239000000203 mixture Substances 0.000 claims abstract description 10
- 102000030523 Catechol oxidase Human genes 0.000 claims abstract description 7
- 108010031396 Catechol oxidase Proteins 0.000 claims abstract description 7
- 230000002255 enzymatic effect Effects 0.000 claims abstract description 6
- 230000002779 inactivation Effects 0.000 claims abstract description 6
- 102000005575 Cellulases Human genes 0.000 claims abstract description 5
- 108010084185 Cellulases Proteins 0.000 claims abstract description 5
- 108090000526 Papain Proteins 0.000 claims abstract description 5
- 239000004365 Protease Substances 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims abstract description 5
- 229940055729 papain Drugs 0.000 claims abstract description 5
- 235000019834 papain Nutrition 0.000 claims abstract description 5
- 239000002002 slurry Substances 0.000 claims abstract description 5
- 239000013049 sediment Substances 0.000 claims description 2
- 238000007670 refining Methods 0.000 abstract description 9
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- 150000008442 polyphenolic compounds Chemical class 0.000 abstract description 5
- 235000013824 polyphenols Nutrition 0.000 abstract description 5
- 239000004382 Amylase Substances 0.000 abstract description 3
- 102000013142 Amylases Human genes 0.000 abstract description 3
- 108010065511 Amylases Proteins 0.000 abstract description 3
- 235000019418 amylase Nutrition 0.000 abstract description 3
- 238000003756 stirring Methods 0.000 abstract description 3
- 235000013399 edible fruits Nutrition 0.000 description 9
- 239000000835 fiber Substances 0.000 description 6
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 4
- 230000029087 digestion Effects 0.000 description 3
- 241000219433 Myrica Species 0.000 description 2
- 240000002834 Paulownia tomentosa Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- -1 hydroxyl radical free radical Chemical class 0.000 description 2
- 230000035922 thirst Effects 0.000 description 2
- 235000007652 Arbutus Nutrition 0.000 description 1
- 240000008327 Arbutus unedo Species 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 235000008375 Decussocarpus nagi Nutrition 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 235000014631 Myrica rubra Nutrition 0.000 description 1
- 235000003283 Pachira macrocarpa Nutrition 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 208000001431 Psychomotor Agitation Diseases 0.000 description 1
- 206010038743 Restlessness Diseases 0.000 description 1
- 240000000203 Salix gracilistyla Species 0.000 description 1
- 240000001085 Trapa natans Species 0.000 description 1
- 235000014364 Trapa natans Nutrition 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000001142 anti-diarrhea Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000021028 berry Nutrition 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 208000001848 dysentery Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 235000009165 saligot Nutrition 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
A kind of extraction and refinement method of high anti-oxidation dietary fiber, its content comprises the following steps：（1）High temperature is passivated：By waxberry dregs 100 DEG C it is xeothermic it is lower keep 10 minutes, make polyphenol oxidase enzymatic inactivation；（2）Ethene softens：Ethene was passed through at the 5th minute, concentration is maintained at 800 1000 ppm, softens fibr tissue；（3）Enzymolysis：By 3:1 solid-liquid ratio addition warm water, while making red bayberry slag-water slurry be cooled to 60 DEG C~80 DEG C, is reacted 30 minutes by 400 450 mg/L addition cellulases while stirring；（4）Complex enzyme hydrolysis：Digested by 0.1%~0.5% addition complex enzyme of waxberry dregs dry powder quality, complex enzyme presses 3 by amylase and papain:1 composition, pH value control during enzymolysis is 6~8, and hydrolysis temperature control is at 60 DEG C~80 DEG C, and enzymolysis time is 60min~90min；（5）Filtration drying：By the mixture filtering after enzymolysis, filter residue drying red bayberry dietary fiber powder.It is an advantage of the invention that：Technological process is simple, and the production time is short, and polyphenols can be preserved fully, and product is in light red, obtains through refining process environmentally safe.
Description
Technical field
It is effective by improving more particularly, to one kind the present invention relates to a kind of method that utilization waxberry dregs obtain through refining dietary fiber
The method that the method for polyphenol substance obtains through refining high anti-oxidation dietary fiber.
Background technology
The subtropical evergreen fruit trees of Myrica(Myrica) Myruca ceas, fruit is that cedductor shape is spherical, there is small nut.General single fruit weight is 10 g
Left and right, belongs to berries.Ming Dynasty's physician's Li Shizhen (1518-1593 A.D.) claim its " shape for example bigcatkin willow son and taste like plum, it is therefore named." red bayberry be China special product
One of, Southeast China each province and the Yunnan-Guizhou Plateau are originated in, cultivation history is long, is the characteristic fruit tree of Chinese Famous, it is widely distributed
In In Middle And Lower Reaches of Changjiang River and all provinces of Southwestern China, there is the province such as Zhejiang, Jiangsu, Fujian, Hunan, Jiangxi and Guangdong major production areas.
In recent years, with industry restructuring and Integrated Development of The Mountainous Region, red bayberry cultivated area expands rapidly, according to incompletely statistics, currently
The total output of China red bayberry is 800,000-100 ten thousand t, the hm of cultivated area about 11.5 ten thousand2.Red bayberry is used as China's characteristic fruit, fruit
Lovely luster, juice is more, sweet and sour palatability, with rich flavor, rich in nutritional ingredients such as multi mineral prime element, vitamin, amino acid,
It is of high nutritive value.According to《Compendium of Materia Medica》Record：Waxberry fruit " can quench the thirst, and the five internal organs, wash stomach, relieving restlessness burst bad odor.Mild-natured, nothing
Poison, with helping digestion, relieve summer heat, cough-relieving of promoting the production of body fluid, aid digestion, antidiarrheal diuresis " the effects such as, since ancient times, red bayberry is used to treatment diarrhoea
And enterogastric diseases, with slaking thirst and help produce saliva, the health-care efficacy such as the preventing or arresting vomiting that helps digestion, treatment dysentery.
At present, China red bayberry is still based on marketing fresh, and the ratio of processing is about 40%, and main converted products includes red bayberry fruit
The products such as juice, red hayberry wine, canned arbutu, candied fruit, have been carried out industrialized production.Produced every year in production substantial amounts of
Waxberry dregs.These waxberry dregs are largely disposed in addition to sub-fraction is used as feed manufacturing, not only cause certain environment dirty
Dye, also results in the wasting of resources.According to the literature in waxberry dregs contain substantial amounts of dietary fiber, its content more than 60% to 70%,
The method that traditional waxberry dregs obtain through refining dietary fiber is extracted using the acid-base method of chemistry, and the method not only have lost substantial amounts of meals
Fiber is eaten, is also destroyed the polyphenols in fiber, therefore the yield of dietary fiber is not high, inoxidizability is relatively low.
Our province red bayberry aboundresources, two ranks degraded by raw material of waxberry dregs through polyphenol oxidase enzymatic inactivation and insoluble fibre
Red bayberry dietary fiber is obtained through refining using enzyme process after section processing, the dietary fiber of high-quality, water soluble dietary fiber content can be obtained
Ratio increase by 20%, inoxidizability increase by 40%, the good advantage of the active strong, effect of the product of exploitation, due to obtain through refining link without
Organic solvent residual, thus with green non-pollution the characteristics of.
The content of the invention
It is an object of the invention to provide a kind of strong high-quality dietary fiber of inoxidizability, while solving the profit of waxberry dregs
With and processing, new pattern is provided for red bayberry comprehensive processing and utilization.
The present invention suppresses the oxidation of polyphenols in waxberry dregs by being passivated the method for polyphenol oxidase, then passes through second
Dietary fiber in the dematuration softening waxberry dregs of alkene, in favor of the degraded of follow-up insoluble diedairy fiber, further lifting
The inoxidizability of red bayberry dietary fiber, obtains the red bayberry dietary fiber of high antioxidant, is achieved through the following technical solutions
's.
（1）High temperature is passivated：By waxberry dregs 100 DEG C it is xeothermic it is lower keep 10 minutes, make polyphenol oxidase enzymatic inactivation；
（2）Ethene softens：Ethene was passed through at the 5th minute, concentration is maintained at 800-1000 ppm, makes fibr tissue soft
Change；
（3）Enzymolysis：By 3:1 solid-liquid ratio addition warm water, while make red bayberry slag-water slurry be cooled to 60 DEG C~80 DEG C, side
Side is stirred to react 30 minutes by 400-450 mg/L addition cellulases；
（4）Complex enzyme hydrolysis：Digested by 0.1%~0.5% addition complex enzyme of waxberry dregs dry powder quality, complex enzyme is by forming sediment
Powder enzyme and papain press 3:1 composition, during enzymolysis pH value control be 6~8, hydrolysis temperature control 60 DEG C~80 DEG C, enzyme
The solution time is 60min~90min；
（5）Filtration drying：By the mixture filtering after enzymolysis, filter residue drying red bayberry dietary fiber powder.
It is an advantage of the invention that：Technological process is simple, and the production time is short, and polyphenols can be preserved fully, and product is in shallow
Red, obtains through refining process environmentally safe.
Embodiment 1
It is a kind of it is of the invention from wooden paulownia and water chestnut red bayberry squeeze the juice after waxberry dregs in obtain through refining the method for dietary fiber, including with
Lower step：
（1）High temperature is passivated：By waxberry dregs 100 DEG C it is xeothermic it is lower keep 10 minutes, make polyphenol oxidase enzymatic inactivation；
（2）Ethene softens：Ethene was passed through at the 5th minute, concentration is maintained at 800 ppm, softens fibr tissue；
（3）Enzymolysis：By 3:1 solid-liquid ratio addition warm water, while making red bayberry slag-water slurry be cooled to 80 DEG C, while stirring
Reacted 30 minutes by 450 mg/L addition cellulases；
（4）Complex enzyme hydrolysis：By waxberry dregs dry powder quality 0.3% addition complex enzyme digested, complex enzyme by amylase with
Papain presses 3:1 composition, pH value control during enzymolysis is 7, and hydrolysis temperature control is at 80 DEG C, and enzymolysis time is 60 mins；
（5）Filtration drying：By the mixture filtering after enzymolysis, filter residue drying red bayberry dietary fiber powder.
Obtained red bayberry dietary fiber accounts for the 92% of waxberry dregs, water-soluble dietary fiber:Insoluble diedairy fiber=1:4.5,
Inoxidizability experiment is higher than Vc and hydroxyl radical free radical.
Embodiment 2
It is a kind of it is of the invention from wooden paulownia and Myrica rubra Dongkui squeeze the juice after waxberry dregs in obtain through refining the method for dietary fiber, including with
Lower step：
（1）High temperature is passivated：By waxberry dregs 100 DEG C it is xeothermic it is lower keep 10 minutes, make polyphenol oxidase enzymatic inactivation；
（2）Ethene softens：Ethene was passed through at the 5th minute, concentration is maintained at 1000 ppm, softens fibr tissue；
（3）Enzymolysis：By 3:1 solid-liquid ratio addition warm water, while making red bayberry slag-water slurry be cooled to 70 DEG C, while stirring
Reacted 30 minutes by 400 mg/L addition cellulases；
（4）Complex enzyme hydrolysis：By waxberry dregs dry powder quality 0.4% addition complex enzyme digested, complex enzyme by amylase with
Papain presses 3:1 composition, pH value control during enzymolysis is 6, and hydrolysis temperature control is at 70 DEG C, and enzymolysis time is 70 mins；
（5）Filtration drying：By the mixture filtering after enzymolysis, filter residue drying red bayberry dietary fiber powder.
Obtained red bayberry dietary fiber accounts for the 95% of waxberry dregs, water-soluble dietary fiber:Insoluble diedairy fiber=1:4, resist
Oxidisability experiment is higher than Vc and hydroxyl radical free radical.
Claims (1)
1. a kind of extraction and refinement method of high anti-oxidation dietary fiber, it is characterised in that comprise the following steps：
Step（1）High temperature is passivated：By waxberry dregs 100 DEG C it is xeothermic it is lower keep 10 minutes, make polyphenol oxidase enzymatic inactivation；
Step（2）Ethene softens：Ethene was passed through at the 5th minute, concentration is maintained at 800-1000 ppm, makes fibr tissue soft
Change；Step（3）Enzymolysis：By 3:1 solid-liquid ratio addition warm water, while make red bayberry slag-water slurry be cooled to 60 DEG C~80 DEG C, side
Side is stirred to react 30 minutes by 400-450 mg/L addition cellulases；
Step（4）Complex enzyme hydrolysis：Digested by 0.1%~0.5% addition complex enzyme of waxberry dregs dry powder quality, complex enzyme is by forming sediment
Powder enzyme and papain press 3:1 composition, during enzymolysis pH value control be 6~8, hydrolysis temperature control 60 DEG C~80 DEG C, enzyme
The solution time is 60min~90min；
Step（5）Filtration drying：By the mixture filtering after enzymolysis, filter residue drying red bayberry dietary fiber powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510245959.6A CN104886605B (en) | 2015-05-15 | 2015-05-15 | A kind of extraction and refinement method of high anti-oxidation dietary fiber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510245959.6A CN104886605B (en) | 2015-05-15 | 2015-05-15 | A kind of extraction and refinement method of high anti-oxidation dietary fiber |
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| Publication Number | Publication Date |
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| CN104886605A CN104886605A (en) | 2015-09-09 |
| CN104886605B true CN104886605B (en) | 2017-10-31 |
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Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100387133C (en) * | 2004-06-09 | 2008-05-14 | 珍奥集团股份有限公司 | Method of preventing oxidation and browning of yacon in processing |
| CN101077182B (en) * | 2007-06-26 | 2011-07-20 | 浙江大学 | Technology for preparing functional dietary fiber through myrica ruba marc enzymolysis |
| CN101744230B (en) * | 2010-01-26 | 2012-05-16 | 浙江扬眉饮品有限公司 | Method for preparing red bayberry dietary fiber powder |
| CN102630781B (en) * | 2012-05-23 | 2013-09-04 | 湖南农业大学 | Method for extracting dietary fiber from tea wastes |
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Effective date of registration: 20170915 Address after: East Lake Nongda road 410128 in Hunan province Changsha Furong District No. 1 Applicant after: Hunan Agricultural University Address before: 410128 life science building, Furong district, Hunan, Changsha Applicant before: Zhang Zhixu |
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