CN104886305A - Method for promoting Tibetan tea water extract to form tea emulsion - Google Patents
Method for promoting Tibetan tea water extract to form tea emulsion Download PDFInfo
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- CN104886305A CN104886305A CN201510336157.6A CN201510336157A CN104886305A CN 104886305 A CN104886305 A CN 104886305A CN 201510336157 A CN201510336157 A CN 201510336157A CN 104886305 A CN104886305 A CN 104886305A
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Abstract
The invention discloses a method for promoting a Tibetan tea water extract to form a tea emulsion. The method comprises the following steps: step 1, a tea water extract or prepared tea powder is dissolved in water to prepare tea soup; step 2, cellulase, pectinase, papain, tannase or combined enzyme of the cellulase, the pectinase, the papain and the tannase are added to the tea soup for treatment, the enzyme treatment temperature is 45 DEG C, and the enzyme treatment time is 40 min; step 3, the content of the tea emulsion is detected, the tea soup after the enzymatic reaction is left to stand overnight at the low temperature of 0-4 DGE C, and the light absorption value under the condition of 640 nm is measured. With adoption of the method, the tea emulsion forming capacity of the prepared tea soup or tea extract is enhanced greatly, and the tea emulsion can be used for preparing milk tea, yogurt and other cloudy beverage and can increase the cloudy degree remarkably.
Description
Technical field
The present invention relates to a kind of promotion and hide the method that tea extract forms tea breast, namely promote by enzyme-added mode the method that in millet paste, tea breast is formed.
Background technology
Hide the founder that tea is China Dark Tea, its manufacture craft is very complicated, generally will pass through and five large operation and the 32 road techniques such as tea, suitable tea, tune tea, ball tea, old tea, about about six months, Yi Gufa concocts product, and standard is hidden dark brownly blackly has light, has red, dense, the exhausted characteristic of alcohol, Chen Si.
Modern medicine study shows: by the Tibetan tea bag of the lasting ferment making of special process containing nearly 500 kinds of organic compounds useful to human body, about 700 kinds of volatile compounds, inorganic matter is also quite abundant, comprises phosphorus, potassium, magnesium, selenium etc. and is no less than 15 kinds of mineral matters.There is following special efficacy: 1. anti-oxidation efficacy; 2. reduce by three height, suppress artery sclerosis effect; 3. radioresistance, anti-sudden change, antiviral efficacy; 4. regulating gastointestinal and improve metabolic effects; 5. removing toxic substances and optimizing water quality effect; 6. comprehensive microelement-supplementing and vitamin.There is the function of many-side, bidirectional modulation owing to hiding tea, make its applicable crowd widely.No matter grow children, no matter fat or thin, tea benefit can be hidden by drinking.
In the processing of hiding tea, because the materials such as Tea Polyphenols and oxide, caffeine, starch, pectin, protein, theaflavin, thearubigin and metal ion are by hydrogen bond, sat linkage, hydrophobic effect and the formation macromolecular substances that condenses together, in external condition, easily form muddiness as when temperature and Acidity of Aikalinity change, the material of this muddiness is referred to as tea breast.And the thick and heavy sense being formed with utilization increase tea beverage of tea breast, it has been found that the tea products of degree of depth fermentation or complete fermentation, the millet paste prepared as black tea and black solution or extract easily produce tea breast, and report by zymotechnic assisted extraction millet paste and the technique preparing tea beverage about studying, but seldom research is related to for the Tibetan tea millet paste or Tibetan tea extraction preparing high tea milk content.
In view of this, the present invention is on the Research foundation of forefathers, and design has been invented a kind of increasing and hidden the method that in tea millet paste, tea breast is formed, thus produces the tea extract being applicable to Cloudy tea liquid beverage or solid beverage, and this case produces thus.
Summary of the invention
The object of the present invention is to provide a kind of promotion to hide the method that tea extract forms tea breast, increasing the content of tea breast in millet paste by adding enzyme to carry out processing in millet paste.
For achieving the above object, the present invention solves the technical scheme of its technical problem and is:
Promote to hide the method that tea extract forms tea breast, comprise the following steps:
Step one, prepare millet paste: by the tealeaves flooding of hiding tea or tea powder that lixiviate is obtained water-soluble, prepare the millet paste that soluble solid content is 0.5-15%;
Step 2, enzyme-addedly carry out enzyme reaction: described millet paste adds cellulase, pectase, papain or their combination ferment treatment, and ferment treatment temperature is 45 DEG C, and enzyme processing time is 40min; Wherein, when cellulase or pectase and papain conbined usage, adopt segment processing technique, namely first with cellulase or/and use Protease Treatment again after pectinase treatment, or first with adding pectase again after Protease Treatment deactivation or/and cellulase process;
Step 3, detection tea milk content: enzyme-added reacted millet paste is put into hold over night in 0 ~ 4 DEG C of low temperature, measures the light absorption value at 640 nm places.
As the preferred embodiment of embodiment, described millet paste adds cellulase process, and cellulase addition is 100 U/mL, after 45 DEG C of insulation 40 min, then 90 DEG C of heating 5 min.
As the preferred embodiment of embodiment, described millet paste adds pectinase treatment, and pectase addition is 300 U/mL, after 45 DEG C of insulation 40 min, then 90 DEG C of heating 5 min.
As the preferred embodiment of embodiment, described millet paste adds Papain ferment treatment, and papain dosage is 400 U/mL, after 45 DEG C of insulation 40 min, then 90 DEG C of heating 5 min.
As the preferred embodiment of embodiment, described millet paste adds cellulase and pectinase treatment, and cellulase addition is 100 U/mL, and pectase addition is 200 U/mL, after 45 DEG C of insulation 40 min, then 90 DEG C of heating 5 min.
As the preferred embodiment of embodiment, described millet paste first adds cellulase process, and cellulase addition is 100 U/mL, after 45 DEG C of insulation 40 min, then add Papain ferment treatment, papain dosage is 400 U/mL, after 45 DEG C of insulation 40 min, then 90 DEG C of heating 5 min.
As the preferred embodiment of embodiment, described millet paste first adds pectinase treatment, and pectase addition is 200 U/mL, after 45 DEG C of insulation 40 min, then add Papain ferment treatment, papain dosage is 400 U/mL, after 45 DEG C of insulation 40 min, then 90 DEG C of heating 5 min.
As the preferred embodiment of embodiment, described millet paste first adds cellulase and pectinase treatment, cellulase addition is 100 U/mL, pectase addition is 200 U/mL, after 45 DEG C of insulation 40 min, then add Papain ferment treatment, papain dosage is 400 U/mL, after 45 DEG C of insulation 40 min, then 90 DEG C of heating 5 min.
After the present invention adopts technique scheme, the ability that obtained millet paste or tea extract form tea breast strengthens greatly, for preparing milk tea, Yoghourt or other pulpy juice, can increase considerably turbidity.
Detailed description of the invention
Embodiments of the invention illustrate technique of the present invention to increase the method that in millet paste, tea breast is formed, but the present invention not only limits the use of in millet paste, is also applicable to the beverage with the addition of tealeaves or tea extract.
Adopt 722N type spectrophotometer in the present invention, under 640 nm, survey the clarity of millet paste.
embodiment 1:
Step one, tea concentrate (concentration is 16.2%) will be hidden be diluted to 1.62%(and hide tea concentrate 1 mL as got, add 9 mL water), under 640 nm, recording absorbance is 0.267.
Step 2, in the Tibetan tea millet paste of dilution, add the cellulase of 0 U/mL, 50 U/mL, 100 U/mL, 150 U/mL, 200 U/mL, 250 U/mL respectively, mix.
Step 3,45 DEG C insulation 40 min after, then 90 DEG C heating 5 min.
Step 4, at 4 DEG C leave standstill a night, record the absorbance of millet paste for being respectively 0.254,0.295,0.356,0.654,0.696,0.712.
embodiment 2:
step one, tea concentrate (concentration is 16.2%) will be hidden be diluted to 1.62%(and hide tea concentrate 1 mL as got, add 9 mL water), under 640 nm, recording absorbance is 0.267.
Step 2, in the Tibetan tea millet paste of dilution, add the pectase of 0 U/mL, 100 U/mL, 200 U/mL, 300 U/mL, 400 U/mL, 500 U/mL respectively, mix.
Step 3,45 DEG C insulation 40 min after, then 90 DEG C heating 5 min.
Step 4, at 4 DEG C leave standstill a night, record the absorbance of millet paste for being respectively 0.254,0.360,0.387,0.599,0.610,0.628.
embodiment 3:
Step one, tea concentrate (concentration is 16.2%) will be hidden be diluted to 1.62%(and hide tea concentrate 1 mL as got, add 9 mL water), under 640 nm, recording absorbance is 0.267.
Step 2, in the Tibetan tea millet paste of dilution, add the papain of 0 U/mL, 200 U/mL, 400 U/mL, 600 U/mL, 800 U/mL, 1000 U/mL respectively, mix.
Step 3,45 DEG C insulation 40 min after, then 90 DEG C heating 5 min.
Step 4, at 4 DEG C leave standstill a night, record the absorbance of millet paste for being respectively 0.254,0.263,0.274,0.299,0.326,0.355.
embodiment 4:
Step one, tea concentrate (concentration is 16.2%) will be hidden be diluted to 1.62%(and hide tea concentrate 1 mL as got, add 9 mL water), under 640 nm, recording absorbance is 0.267.
Step 2, in the Tibetan tea millet paste of dilution, add the tannase of 0 U/mL, 0.03 U/mL, 0.06 U/mL, 0.09 U/mL, 0.12 U/mL, 0.15 U/mL respectively, mix.
Step 3,45 DEG C insulation 40 min after, then 90 DEG C heating 5 min.
Step 4, at 4 DEG C leave standstill a night, record the absorbance of millet paste for being respectively 0.254,0.260,0.267,0.281,0.287,0.293.
embodiment 5:
Step one, tea concentrate (concentration is 16.2%) will be hidden be diluted to 1.62%(and hide tea concentrate 1 mL as got, add 9 mL water), under 640 nm, recording absorbance is 0.267.
Step 2, in the Tibetan tea millet paste of dilution, add the cellulase+pectase of 0 U/mL, 100 U/mL+200 U/mL respectively, mix.
Step 3,45 DEG C insulation 40 min after, then 90 DEG C heating 5 min.
Step 4, at 4 DEG C leave standstill a night, record the absorbance of millet paste for being respectively 0.254,0.801.
embodiment 6:
Step one, tea concentrate (concentration is 16.2%) will be hidden be diluted to 1.62%(and hide tea concentrate 1 mL as got, add 9 mL water), under 640 nm, recording absorbance is 0.267.
Step 2, in the Tibetan tea millet paste of dilution, add the cellulase+tannase of 0 U/mL, 100 U/mL+0.09 U/mL respectively, mix.
Step 3,45 DEG C insulation 40 min after, then 90 DEG C heating 5 min.
Step 4, at 4 DEG C leave standstill a night, record the absorbance of millet paste for being respectively 0.254,0.368.
embodiment 7:
Step one, tea concentrate (concentration is 16.2%) will be hidden be diluted to 1.62%(and hide tea concentrate 1 mL as got, add 9 mL water), under 640 nm, recording absorbance is 0.267.
Step 2, in the Tibetan tea millet paste of dilution, add the pectase+tannase of 0 U/mL, 200 U/mL+0.09 U/mL respectively, mix.
Step 3,45 DEG C insulation 40 min after, then 90 DEG C heating 5 min.
Step 4, at 4 DEG C leave standstill a night, record the absorbance of millet paste for being respectively 0.254,0.610.
embodiment 8:
Step one, tea concentrate (concentration is 16.2%) will be hidden be diluted to 1.62%(and hide tea concentrate 1 mL as got, add 9 mL water), under 640 nm, recording absorbance is 0.267.
Step 2, in the Tibetan tea millet paste of dilution, first add the cellulase of 0 U/mL, 100 U/mL, after 45 DEG C of insulation 40 min, then add the papain of 0 U/mL, 400 U/mL, mix.
Step 3,45 DEG C insulation 40 min after, then 90 DEG C heating 5 min.
Step 4, at 4 DEG C leave standstill a night, the absorbance recording millet paste is respectively 0.254,0.598.
embodiment 9:
Step one, tea concentrate (concentration is 16.2%) will be hidden be diluted to 1.62%(and hide tea concentrate 1 mL as got, add 9 mL water), under 640 nm, recording absorbance is 0.267.
Step 2, in the Tibetan tea millet paste of dilution, first add the pectase of 0 U/mL, 200 U/mL, after 45 DEG C of insulation 40 min, then add the papain of 0 U/mL, 400 U/mL, mix.
Step 3,45 DEG C insulation 40 min after, then 90 DEG C heating 5 min.
Step 4, at 4 DEG C leave standstill a night, the absorbance recording millet paste is respectively 0.254,0.628.
embodiment 10:
Step one, tea concentrate (concentration is 16.2%) will be hidden be diluted to 1.62%(and hide tea concentrate 1 mL as got, add 9 mL water), under 640 nm, recording absorbance is 0.267.
Step 2, in the Tibetan tea millet paste of dilution, first add the tannase of 0 U/mL, 0.09 U/mL, after 45 DEG C of insulation 40 min, then add the papain of 0 U/mL, 400 U/mL, mix.
Step 3,45 DEG C insulation 40 min after, then 90 DEG C heating 5 min.
Step 4, at 4 DEG C leave standstill a night, the absorbance recording millet paste is respectively 0.254,0.328.
embodiment 11:
Step one, tea concentrate (concentration is 16.2%) will be hidden be diluted to 1.62%(and hide tea concentrate 1 mL as got, add 9 mL water), under 640 nm, recording absorbance is 0.267.
Step 2, in the Tibetan tea millet paste of dilution, first add the cellulase+pectase of 0 U/mL, 100 U/mL+200 U/mL, after 45 DEG C of insulation 40 min, then add the papain of 0 U/mL, 400 U/mL, mix.
Step 3,45 DEG C insulation 40 min after, then 90 DEG C heating 5 min.
Step 4, at 4 DEG C leave standstill a night, record the absorbance of millet paste for being respectively 0.254,0.827.
embodiment 12:
Step one, tea concentrate (concentration is 16.2%) will be hidden be diluted to 1.62%(and hide tea concentrate 1 mL as got, add 9 mL water), under 640 nm, recording absorbance is 0.267.
Step 2, in the Tibetan tea millet paste of dilution, first add the cellulase+tannase of 0 U/mL, 100 U/mL+0.09 U/mL, after 45 DEG C of insulation 40 min, then add the papain of 0 U/mL, 400 U/mL, mix.
Step 3,45 DEG C insulation 40 min after, then 90 DEG C heating 5 min.
Step 4, at 4 DEG C leave standstill a night, record the absorbance of millet paste for being respectively 0.254,0.431.
embodiment 13:
Step one, tea concentrate (concentration is 16.2%) will be hidden be diluted to 1.62%(and hide tea concentrate 1 mL as got, add 9 mL water), under 640 nm, recording absorbance is 0.267.
Step 2, in the Tibetan tea millet paste of dilution, first add the pectase+tannase of 0 U/mL, 200 U/mL+0.09 U/mL, after 45 DEG C of insulation 40 min, then add the papain of 0 U/mL, 400 U/mL, mix.
Step 3,45 DEG C insulation 40 min after, then 90 DEG C heating 5 min.
Step 4, at 4 DEG C leave standstill a night, record the absorbance of millet paste for being respectively 0.254,0.417.
embodiment 14:
Step one, tea concentrate (concentration is 16.2%) will be hidden be diluted to 1.62%(and hide tea concentrate 1 mL as got, add 9 mL water), under 640 nm, recording absorbance is 0.267.
Step 2, in the Tibetan tea millet paste of dilution, first add the cellulase+pectase+tannase of 0 U/mL, 100 U/mL+200 U/mL+0.09 U/mL, after 45 DEG C of insulation 40 min, then add the papain of 0 U/mL, 400 U/mL, mix.
Step 3,45 DEG C insulation 40 min after, then 90 DEG C heating 5 min.
Step 4, at 4 DEG C leave standstill a night, record the absorbance of millet paste for being respectively 0.254,0.563.
After the present invention adopts technique scheme, namely pectase, cellulase, protease or their Combined Treatment is adopted, the tea breast in millet paste can be increased, and adopt tannase process can not increase the amount of tea breast, compared with conventional thought, the present invention is the tea breast improved in millet paste, instead of in order to the clarity that improves millet paste or remove tea breast.Namely the present inventor adopts reverse thinking, overcomes the prejudice of prior art, and the tea adopting ferment treatment to increase in millet paste is newborn, can be widely used in preparation milk tea, Yoghourt or other pulpy juice.
The above, be only present pre-ferred embodiments, therefore can not limit scope of the invention process according to this, the equivalence change namely done according to the scope of the claims of the present invention and description with modify, all should still belong in scope that the present invention contains.
Claims (8)
1. promote to hide the method that tea extract forms tea breast, it is characterized in that: comprise the following steps:
Step one, prepare millet paste: by the tealeaves flooding of hiding tea or tea powder that lixiviate is obtained water-soluble, prepare the millet paste that soluble solid content is 0.5-15%;
Step 2, enzyme-addedly carry out enzyme reaction: described millet paste adds cellulase, pectase, papain or their combination ferment treatment, and ferment treatment temperature is 45 DEG C, and enzyme processing time is 40min; Wherein, when cellulase or pectase and papain conbined usage, adopt segment processing technique, namely first with cellulase or/and use Protease Treatment again after pectinase treatment, or first with adding pectase again after Protease Treatment deactivation or/and cellulase process;
Step 3, detection tea milk content: enzyme-added reacted millet paste is put into hold over night in 0 ~ 4 DEG C of low temperature, measures the light absorption value at 640nm place.
2. a kind of promotion as claimed in claim 1 hides the method that tea extract forms tea breast, and it is characterized in that: described millet paste adds cellulase process, cellulase addition is 100 U/mL, after 45 DEG C of insulation 40 min, then 90 DEG C of heating 5 min.
3. a kind of promotion as claimed in claim 1 hides the method that tea extract forms tea breast, and it is characterized in that: described millet paste adds pectinase treatment, pectase addition is 300 U/mL, after 45 DEG C of insulation 40 min, then 90 DEG C of heating 5 min.
4. a kind of promotion as claimed in claim 1 hides the method that tea extract forms tea breast, and it is characterized in that: described millet paste adds Papain ferment treatment, papain dosage is 400 U/mL, after 45 DEG C of insulation 40 min, then 90 DEG C of heating 5 min.
5. a kind of promotion as claimed in claim 1 hides the method that tea extract forms tea breast, it is characterized in that: described millet paste adds cellulase and pectinase treatment, cellulase addition is 100 U/mL, pectase addition is 200 U/mL, after 45 DEG C of insulation 40 min, then 90 DEG C of heating 5 min.
6. a kind of promotion as claimed in claim 1 hides the method that tea extract forms tea breast, it is characterized in that: described millet paste first adds cellulase process, cellulase addition is 100 U/mL, after 45 DEG C of insulation 40 min, add Papain ferment treatment again, papain dosage is 400 U/mL, after 45 DEG C of insulation 40 min, then 90 DEG C of heating 5 min.
7. a kind of promotion as claimed in claim 1 hides the method that tea extract forms tea breast, it is characterized in that: described millet paste first adds pectinase treatment, pectase addition is 200 U/mL, after 45 DEG C of insulation 40 min, add Papain ferment treatment again, papain dosage is 400 U/mL, after 45 DEG C of insulation 40 min, then 90 DEG C of heating 5 min.
8. a kind of promotion as claimed in claim 1 hides the method that tea extract forms tea breast, it is characterized in that: described millet paste first adds cellulase and pectinase treatment, cellulase addition is 100 U/mL, pectase addition is 200 U/mL, after 45 DEG C of insulation 40 min, then add Papain ferment treatment, papain dosage is 400 U/mL, after 45 DEG C of insulation 40 min, then 90 DEG C of heating 5 min.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105379875A (en) * | 2015-12-22 | 2016-03-09 | 四川藏匠茶业股份有限公司 | In-bulk Tibet tea and preparing method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696811A (en) * | 2011-12-02 | 2012-10-03 | 湖南梅山黑茶有限公司 | Preparation method of instant black tea |
| CN103798438A (en) * | 2014-02-18 | 2014-05-21 | 福建泉州瑞龙茶业有限公司 | Preparation method of pure tea beverage |
| CN103999975A (en) * | 2014-05-22 | 2014-08-27 | 青海阿如拉藏医药研究开发有限公司 | Preparation method for instant Tibetan tea |
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- 2015-06-17 CN CN201510336157.6A patent/CN104886305B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696811A (en) * | 2011-12-02 | 2012-10-03 | 湖南梅山黑茶有限公司 | Preparation method of instant black tea |
| CN103798438A (en) * | 2014-02-18 | 2014-05-21 | 福建泉州瑞龙茶业有限公司 | Preparation method of pure tea beverage |
| CN103999975A (en) * | 2014-05-22 | 2014-08-27 | 青海阿如拉藏医药研究开发有限公司 | Preparation method for instant Tibetan tea |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105379875A (en) * | 2015-12-22 | 2016-03-09 | 四川藏匠茶业股份有限公司 | In-bulk Tibet tea and preparing method thereof |
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