CN104880553A - Kit for detecting residual ivermectin in beef - Google Patents
Kit for detecting residual ivermectin in beef Download PDFInfo
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- CN104880553A CN104880553A CN201510294872.8A CN201510294872A CN104880553A CN 104880553 A CN104880553 A CN 104880553A CN 201510294872 A CN201510294872 A CN 201510294872A CN 104880553 A CN104880553 A CN 104880553A
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- ivermectin
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- residual
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Pharmacology & Pharmacy (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses a kit for detecting residual ivermectin in beef. The kit for detecting the residual ivermectin in the beef comprises a box type shell and an immunochromatography test strip, wherein the immunochromatography test strip is positioned in the box type shell and comprises a sample pad, an analyzing membrane, a water absorbing pad, an adhesive bottom lining and an anti-ivermectin monoclonal antibody combining pad; an up-converting phosphor material particle mark coats the ivermectin monoclonal antibody resistant combining pad; the adhesive bottom lining is fixedly adhered to the bottom of the box type shell; the analyzing membrane is adhered to the adhesive bottom lining; the combining pad and the water absorbing pad are respectively adhered to two sides of the analyzing membrane; a sample pad is adhered to one side, which is far away from the analyzing membrane, of the combining pad; a detecting strap and a quality control strap are arranged on the analyzing membrane; anti-ivermectin monoclonal antibodies coat the detecting strap; goat anti-mouse lgG coats the quality control strap; and the box type shell is provided with a sample adding hole, a result reading window and an end point indicating window which respectively correspond to the sample pad, the analyzing membrane and the water absorbing pad. An up-converting phosphor technology and an immunological technology are combined. The kit is suitable for detecting the residual ivermectin in the beef on the spot quickly, is low in cost, and can detect the residual ivermectin in the beef accurately, quickly and quantitatively.
Description
Technical field
The present invention relates to field of medical technology, be specifically related to a kind ofly detect the residual kit of ivermectin in beef.
Background technology
Ivermectin is a kind of semi-synthetic macrolide antibiotics, its toxicity is significantly less than Avermectin, for current veterinary clinic uses maximum antiparasitic agents, to endoparasite and ectoparasite particularly nematode and arthropod all have and good kill effect, be used in widely in the domestic animals such as pig, ox, sheep.Ivermectin has genotoxicity, and in animal body the residence time long, long-term exposure on human has serious health the risk of harm, and has hypertoxicity to aquatic environment.
At present the detection method of ivermectin is mainly contained (ELISA) such as high performance liquid chromatography (HPLC), Liquid Chromatography/Mass Spectrometry (LC-MS), euzymelinked immunosorbent assay (ELISA).But the sample pre-treatments of HPLC and LC-MS method is complicated, complex operation, and this expensive equipment needed for two kinds of methods, cost is high, is unsuitable for the examination to great amount of samples.And false positive easily appears in ELISA method, cause the deviation of result.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides and a kind ofly detect the residual kit of ivermectin in beef, this kit turns luminescence technology on adopting and is combined with immunological technique, suitable quick detection at the scene, cost is low, be suitable for the examination to great amount of samples, the feature having accurately, fast, quantitatively detect.
Object of the present invention carrys out specific implementation by the following technical programs:
A kind ofly detect the residual kit of ivermectin in beef, comprise case type shell and be positioned at the immuno-chromatographic test paper strip of box case inside, described immuno-chromatographic test paper strip comprises sample pad, analyzing film, adsorptive pads, viscosity end liner and be coated with the pad of anti-ivermectin monoclonal antibody of forwarding luminescent material particle marker, described viscosity end liner gummed is fixed on the bottom of case type shell, analyzing film is glued on viscosity end liner, pad and adsorptive pads are glued at the both sides of analyzing film respectively, described sample pad is glued at the side of pad away from analyzing film, analyzing film is provided with and wraps by the detection zone of anti-ivermectin monoclonal antibody and wrap by the quality control band of sheep anti mouse lgG, described case type shell is provided with respectively with sample pad, analyzing film and sample added holes corresponding to adsorptive pads, result reads window and End point indication window.
Further, described analyzing film is nitrocellulose filter.
Further, described box case is made up of transparent material.
The present invention is a kind of detects the kit that in beef, ivermectin is residual, the pad of this kit is coated with the anti-ivermectin monoclonal antibody of forwarding luminescent material particle marker, be provided with on analyzing film simultaneously and wrap by the detection zone of anti-ivermectin monoclonal antibody and wrap by the quality control band of sheep anti mouse lgG, ivermectin and two strain antibody reaction bonded in sample during reaction, detection zone formed ivermectin protein antibodies-ivermectin proteantigen-on forward the ivermectin protein antibodies compound of light particle marker, on turn light-emitting particles at infrared ray excited lower transmitting visible ray, radiative intensity is directly proportional to the concentration of ivermectin albumen in sample.No matter whether there is ivermectin in sample, quality control band all can be formed sheep anti mouse-on forward the anti-ivermectin antibody complex of light particle marker, utilization forwards light immunity analysis instrument and scanning analysis is carried out to test strips, display measurement result.This kit turns luminescence technology on adopting and is combined with immunological technique, suitable quick detection at the scene, and cost is low, is suitable for the examination to great amount of samples, the feature having accurately, fast, quantitatively detect.
Accompanying drawing explanation
According to drawings and embodiments the present invention is described in further detail below.
Fig. 1 is a kind of stereographic map detecting the kit that ivermectin in beef remains of the present invention;
Fig. 2 is the structural representation of immuno-chromatographic test paper strip.
In figure: 1-case type shell; 2-sample pad; 3-analyzing film; 4-adsorptive pads; 5-viscosity end liner; 6-pad; 7-detection zone; 8-quality control band; 9-sample added holes; 10-result reads window; 11-End point indication window.
Embodiment
As shown in Figure 1-2, a kind of described in the embodiment of the present invention detects the kit that in beef, ivermectin is residual, comprise case type shell 1 and be positioned at the immuno-chromatographic test paper strip of box case 1 inside, immuno-chromatographic test paper strip comprises sample pad 2, analyzing film 3, adsorptive pads 4, viscosity end liner 5 and be coated with the pad 6 of anti-ivermectin monoclonal antibody of forwarding luminescent material particle marker, viscosity end liner 5 gummed is fixed on the bottom of case type shell 1, analyzing film 3 is glued on viscosity end liner 5, pad 6 and adsorptive pads 4 are glued at the both sides of analyzing film 3 respectively, sample pad 2 is glued at the side of pad 6 away from analyzing film 3, analyzing film 3 is provided with and wraps by the detection zone 7 of anti-ivermectin monoclonal antibody and wrap by the quality control band 8 of sheep anti mouse lgG, case type shell 1 is provided with respectively with sample pad 2, the sample added holes 9 of analyzing film 3 and adsorptive pads 4 correspondence, result reads window 10 and End point indication window 11.
Preferred embodiment, analyzing film 3 is nitrocellulose filter to one.
Preferably, box case 1 is made up of transparent material, is convenient to observation.
The present invention is a kind of detects the kit that in beef, ivermectin is residual, in use, add the ivermectin in sample and two strain antibody reaction bonded, detection zone formed ivermectin protein antibodies-ivermectin proteantigen-on forward the ivermectin protein antibodies compound of light particle marker, on turn light-emitting particles at infrared ray excited lower transmitting visible ray, radiative intensity is directly proportional to the concentration of ivermectin albumen in sample.No matter whether there is ivermectin in sample, quality control band all can be formed sheep anti mouse-on forward the anti-ivermectin antibody complex of light particle marker.Utilization forwards light immunity analysis instrument and scanning analysis is carried out to test strips, display measurement result, in whole testing process, provide liquid to flow through the power of whole test strips by syphonic effect.
This kit turns luminescence technology on adopting and is combined with immunological technique, suitable quick detection at the scene, and cost is low, is suitable for the examination to great amount of samples, the feature having accurately, fast, quantitatively detect.
Last it is noted that the foregoing is only the preferred embodiments of the present invention; be not limited to the present invention; for a person skilled in the art; it still can regulate the technical scheme described in foregoing embodiments; or equivalent replacement is carried out to wherein portion of techniques feature; within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (3)
1. one kind is detected the kit that in beef, ivermectin is residual, comprise case type shell and be positioned at the immuno-chromatographic test paper strip of box case inside, it is characterized in that: described immuno-chromatographic test paper strip comprises sample pad, analyzing film, adsorptive pads, viscosity end liner and be coated with the pad of anti-ivermectin monoclonal antibody of forwarding luminescent material particle marker, described viscosity end liner gummed is fixed on the bottom of case type shell, analyzing film is glued on viscosity end liner, pad and adsorptive pads are glued at the both sides of analyzing film respectively, described sample pad is glued at the side of pad away from analyzing film, analyzing film is provided with and wraps by the detection zone of anti-ivermectin monoclonal antibody and wrap by the quality control band of sheep anti mouse lgG, described case type shell is provided with respectively with sample pad, analyzing film and sample added holes corresponding to adsorptive pads, result reads window and End point indication window.
2. according to claim 1ly a kind ofly detect the residual kit of ivermectin in beef, it is characterized in that: described analyzing film is nitrocellulose filter.
3. according to claim 1 and 2ly a kind ofly detect the residual kit of ivermectin in beef, it is characterized in that: described box case is made up of transparent material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510294872.8A CN104880553A (en) | 2015-06-02 | 2015-06-02 | Kit for detecting residual ivermectin in beef |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510294872.8A CN104880553A (en) | 2015-06-02 | 2015-06-02 | Kit for detecting residual ivermectin in beef |
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| Publication Number | Publication Date |
|---|---|
| CN104880553A true CN104880553A (en) | 2015-09-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510294872.8A Pending CN104880553A (en) | 2015-06-02 | 2015-06-02 | Kit for detecting residual ivermectin in beef |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1690711A (en) * | 2004-04-23 | 2005-11-02 | 中国人民解放军军事医学科学院微生物流行病研究所 | Immune chromatographic test paper bar based on up conversion luminescence technology |
| CN1811440A (en) * | 2006-02-17 | 2006-08-02 | 中国农业大学 | Method for detecting Ivermectin and special enzyme-linked immune reagent kit thereof |
| CN202330436U (en) * | 2011-11-08 | 2012-07-11 | 黑龙江八一农垦大学 | Selenium marker test paper for detecting Avermectin residues |
| CN103675287A (en) * | 2013-12-12 | 2014-03-26 | 洛阳莱普生信息科技有限公司 | Ivermectin chemiluminescence enzyme-linked immunoassay rapid detection kit |
| CN204631044U (en) * | 2015-06-02 | 2015-09-09 | 中国农业科学院兰州畜牧与兽药研究所 | A kind ofly detect the residual kit of ivermectin in beef |
-
2015
- 2015-06-02 CN CN201510294872.8A patent/CN104880553A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1690711A (en) * | 2004-04-23 | 2005-11-02 | 中国人民解放军军事医学科学院微生物流行病研究所 | Immune chromatographic test paper bar based on up conversion luminescence technology |
| CN1811440A (en) * | 2006-02-17 | 2006-08-02 | 中国农业大学 | Method for detecting Ivermectin and special enzyme-linked immune reagent kit thereof |
| CN202330436U (en) * | 2011-11-08 | 2012-07-11 | 黑龙江八一农垦大学 | Selenium marker test paper for detecting Avermectin residues |
| CN103675287A (en) * | 2013-12-12 | 2014-03-26 | 洛阳莱普生信息科技有限公司 | Ivermectin chemiluminescence enzyme-linked immunoassay rapid detection kit |
| CN204631044U (en) * | 2015-06-02 | 2015-09-09 | 中国农业科学院兰州畜牧与兽药研究所 | A kind ofly detect the residual kit of ivermectin in beef |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150902 |
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| RJ01 | Rejection of invention patent application after publication |