CN104877904B - A kind of bacterium disperses calculating instrument - Google Patents
A kind of bacterium disperses calculating instrument Download PDFInfo
- Publication number
- CN104877904B CN104877904B CN201510214710.9A CN201510214710A CN104877904B CN 104877904 B CN104877904 B CN 104877904B CN 201510214710 A CN201510214710 A CN 201510214710A CN 104877904 B CN104877904 B CN 104877904B
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- China
- Prior art keywords
- detection light
- light pond
- cabinet
- test tube
- pond
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48735—Investigating suspensions of cells, e.g. measuring microbe concentration
Abstract
The invention discloses a kind of bacterium to disperse calculating instrument, including cabinet, the cabinet includes the head cover that can push down test tube, injection port is additionally provided with the cabinet, detection light pond is provided with injection port lower section in the cabinet, wherein the lower end in the detection light pond is provided with transducer, the right end in the detection light pond is provided with towards the laser emitter on the inside of detection light pond, the left end in the detection light pond is provided with ligh trap, wherein the rear end of the cabinet is provided with supersonic generator;The calculating instrument passes through rational structural improvement, sound wave is produced especially by supersonic generator, and the vibrations for coordinating transducer to produce certain frequency form ultrasonic wave, ultrasonic wave is delivered in test tube, cavitation effect is produced in a liquid, so as to which the thalline to clump together originally in liquid be broken up, and turbidity is detected using 90 degree of scatterings of laser, confirm dispersion effect.
Description
Technical field
The present invention relates to Bioexperiment field of tool, particularly a kind of scattered calculating instrument of directed toward bacteria experiment detection.
Background technology
It is generally known that since microbial body is typically agglomerating existing, such as with reference to mycobacteria, therefore it is relevant doing
Need to try to make these poly- pockets of bacterial mass disperse in the solution when experiment.And currently used process for dispersing is to use
Mechanical lapping is sent out, and for example with mill bacterium bottle, mill tube etc., these operations need to carry out by hand, not only take time and effort, Er Qiefen
It is unstable to dissipate effect, and then influences experiment effect.And since this generic operation must operate in unlimited container by hand, for
The often bacterium with infectiousness and toxicity again, be likely to the skin contact with operating personnel in grinding and dilution
To or inspiration go, it is dangerous greatly.
After the completion of bacterial mass disperses, it is necessary to carry out analysis test, requirement of the test common at present to instrument, vessel
Strictly, its operation is caused to become trouble, referring for example to Patent No. 200710067170.1《Direct microscopic method milk body is thin
Born of the same parents/bacterium automatic counter for counting》, its structure is excessively complicated, have impact on the popularization and application of technology.
The content of the invention
For overcome the deficiencies in the prior art, present invention offer one kind uses more convenient reliable bacterium and disperses to count
Instrument.
The technical solution adopted by the present invention to solve the technical problems is:
A kind of bacterium disperses calculating instrument, including cabinet, and the cabinet includes the head cover that can push down test tube, on the cabinet
Injection port is additionally provided with, detection light pond is provided with injection port lower section in the cabinet, wherein under the detection light pond
End is provided with transducer, and the right end in the detection light pond is provided with towards the laser emitter on the inside of detection light pond, the detection
The left end in light pond is provided with ligh trap, and detection light pond front end is provided with detection circuit, wherein the rear end of the cabinet is provided with
Supersonic generator.
As a preference, the left end of the ligh trap is provided with Brewster's angle.
As a preference, shielding case is provided between the supersonic generator and detection light pond, reduces ultrasonic wave
Interference of the generator to reading.
As a preference, the front end in the detection light pond is provided with silicon photoelectric diode amplifying circuit.
As a preference, the adapter that can push down test tube is provided with injection port position in the head cover, its
Described in adapter include installed part, cup lid group and the sub-assembly positioned at inner side successively, wherein the installed part includes use
In the upper mounting blocks being connected with head cover and the upper outer sleeve portion positioned at upper mounting blocks, the cup lid group includes positioned at upper installation
Lower mounting blocks at block lower position and the inner sleeve canister portion on the inside of outer sleeve portion, wherein the upper end extension in the inner sleeve portion
To outer sleeve portion upper end position, the sub-assembly is located on the inside of inner sleeve canister portion upper end, between the sub-assembly and inner sleeve canister portion
It is provided with elastic ring.
As a preference, detection light pond is stretched into the upper end of the transducer, wherein the upper end of the transducer is set
It is provided with test tube location hole.
As a preference, the bullet for spring installation is provided between the lower mounting blocks and inner sleeve canister portion of the cup lid group
Spring cavity, the outer sleeve portion upper end of the installed part are provided with flange.
The beneficial effects of the invention are as follows:The calculating instrument is produced by rational structural improvement especially by supersonic generator
Raw sound wave, and the vibrations for coordinating transducer to produce certain frequency form ultrasonic wave, ultrasonic wave is delivered in test tube, is produced in a liquid
Raw cavitation effect, so that the thalline to clump together originally in liquid be broken up, and detects turbidity using 90 degree of scatterings of laser,
Confirm dispersion effect, hand-ground process is both not required in whole step, and directly can be carried out reading, and effect carries significantly
It is high.
Brief description of the drawings
The present invention is further described with reference to the accompanying drawings and examples.
Fig. 1 is the stereogram of the present invention;
Fig. 2 is the internal structure schematic diagram of the present invention;
Fig. 3 is the partial sectional view in detection light pond in the present invention;
Fig. 4 is the partial sectional perspective view of the adapter of head cover in the present invention.
Embodiment
With reference to Fig. 1, Fig. 2, Fig. 3, a kind of bacterium disperses calculating instrument, including cabinet 1, and the cabinet 1, which includes, can push down examination
The head cover 2 of pipe, is additionally provided with injection port 21 on the cabinet 1, is inserted into for test tube 11.It is located in the cabinet 1 under injection port 21
Square place is provided with detection light pond 3, the environment as a detection turbidity.The lower end in wherein described detection light pond 3 is provided with transducing
Device 4, wherein transducer 4 are as needed, its size model number can flexibly be chosen.The right end in the detection light pond 3 is provided with towards inspection
The laser emitter 5 of the inner side of light pond 3 is surveyed to provide light source.The left end in the detection light pond 3 is provided with ligh trap 6, as an envelope
The firmly device of light, avoiding photon from being reflected back detection light pond 3 influences accuracy of detection.3 front end of detection light pond is provided with detection
Circuit calculates liquid turbidity to read the data that the reflection liquid from test tube is come in, you can know number of bacteria, wherein
The rear end of the cabinet 1 is provided with supersonic generator 7, sends ul-trasonic irradiation in test tube wall by supersonic generator 7, just
Can be broken up the bacterial mass in test tube.
As another embodiment, a kind of bacterium disperses calculating instrument, including cabinet 1, and the cabinet 1 includes and can push down
The head cover 2 of test tube, is additionally provided with injection port 21 on the cabinet 1, is inserted into for test tube 11.It is located at injection port 21 in the cabinet 1
Detection light pond 3 is provided with lower section, the environment as a detection turbidity.The lower end in wherein described detection light pond 3, which is provided with, to be changed
Energy device 4, the right end in the detection light pond 3 are provided with the laser emitter 5 towards the inner side of detection light pond 3, the detection light pond 3
Left end is provided with ligh trap 6, and 3 front end of detection light pond is provided with detection circuit, wherein the rear end of the cabinet 1 is provided with ultrasound
Wave producer 7, wherein the left end of the ligh trap 6 is provided with Brewster's angle 8, can play the more preferable effect for eliminating veiling glare, match somebody with somebody
The laser for closing 90 degree of scatterings that laser emitter 5 is launched carrys out liquid turbidity in test tubes.
As another embodiment, a kind of bacterium disperses calculating instrument, including cabinet 1, and the cabinet 1 includes and can push down
The head cover 2 of test tube, is additionally provided with injection port 21 on the cabinet 1, is inserted into for test tube 11.It is located at injection port 21 in the cabinet 1
Detection light pond 3 is provided with lower section, the environment as a detection turbidity.The lower end in wherein described detection light pond 3, which is provided with, to be changed
Energy device 4, the right end in the detection light pond 3 are provided with the laser emitter 5 towards the inner side of detection light pond 3, the detection light pond 3
Left end is provided with ligh trap 6, and 3 front end of detection light pond is provided with detection circuit, wherein the rear end of the cabinet 1 is provided with ultrasound
Wave producer 7, shielding case 71 is provided between the supersonic generator 7 and detection light pond 3, is disturbed for shielding, is avoided surpassing
Sonic generator 7 interferes detection circuit, influences detection result, and the front end in the detection light pond 3 is provided with silicon photoelectricity two
Pole pipe amplifying circuit 31, accuracy of detection is improved for amplified signal.
With reference to Fig. 4, it is bonded closely to ensure test tube 11 with transducer 4 to ensure effect, is located at sample introduction in the head cover 2
Mouth is provided with the adapter 9 that can push down test tube 11 at 21 positions.Wherein described adapter 9 includes installed part 91, traps successively
Part 92 and the sub-assembly 93 positioned at inner side, wherein the installed part 91 include upper mounting blocks 94 for being connected with head cover 2 and
Positioned at the upper outer sleeve portion 95 of upper mounting blocks 94, under the cup lid group 92 includes at upper 94 lower position of mounting blocks
Mounting blocks 96 and the inner sleeve canister portion 97 positioned at the inner side of outer sleeve portion 95, wherein the upper end in the inner sleeve portion 97 extends to outer sleeve
At 95 upper end position of portion, the sub-assembly 93 is located on the inside of 97 upper end of inner sleeve canister portion, the sub-assembly 93 and inner sleeve canister portion 97 it
Between be provided with elastic ring 98, enable adapter 9 according to the gauge size of test tube 11 replace sub-assembly 93.The transducer 4 it is upper
Detection light pond 3 is stretched at end, wherein the upper end of the transducer 4, which is set, is provided with test tube location hole, the insertion of test tube 11 can stick into
Test tube location hole positions, and ensures that test tube 11 can more closely touch transducer 4 to ensure effect.The lower installation of the cup lid group 92
The spring plenum length 99 for spring installation, 95 upper end of outer sleeve portion of the installed part 91 are provided between block 96 and inner sleeve canister portion 97
Flange 90 is provided with, by the spring in spring plenum length 99, it is ensured that head cover 2 can apply test tube 11 enough lower pressure.
According to above-mentioned principle, the present invention can also carry out the above embodiment appropriate change and modification.Therefore, this hair
It is bright to be not limited to embodiment disclosed and described above, some modifications and changes of the present invention should also be as falling into this
In the scope of the claims of invention.
Claims (1)
1. a kind of bacterium disperses calculating instrument, including cabinet (1), the cabinet (1) includes the head cover (2) that can press test tube, institute
State and injection port (21) be additionally provided with cabinet (1), it is characterised in that:Set in the cabinet (1) at injection port (21) lower section
Detection light pond (3) is equipped with, wherein the lower end in the detection light pond (3) is provided with transducer (4), the right side of the detection light pond (3)
End, which is provided with towards the laser emitter (5) on the inside of detection light pond (3), the left end of the detection light pond (3), is provided with ligh trap
(6), detection light pond (3) front end is provided with detection circuit, wherein the rear end of the cabinet (1) is provided with supersonic generator
(7), the left end of the ligh trap (6) is provided with Brewster's angle (8), between the supersonic generator (7) and detection light pond (3)
Shielding case (71) is provided with, the front end of the detection light pond (3) is provided with silicon photoelectric diode amplifying circuit (31), the head cover
(2) be provided with the adapter (9) that can press test tube (11) at injection port (21) position in, wherein the adapter (9) according to
It is secondary to include installed part (91), cup lid group (92) and the sub-assembly (93) positioned at inner side, wherein the installed part (91) includes
Outer sleeve portion (95) for the upper mounting blocks (94) being connected with head cover (2) and on upper mounting blocks (94), the cup lid group
(92) lower mounting blocks (96) at upper mounting blocks (94) lower position and interior on the inside of outer sleeve portion (95) are included
Sleeve part (97), wherein the upper end in the inner sleeve portion (97) is extended at outer sleeve portion (95) upper end position, the sub-assembly
(93) on the inside of inner sleeve canister portion (97) upper end, elastic ring is provided between the sub-assembly (93) and inner sleeve canister portion (97)
(98), detection light pond (3) is stretched into the upper end of the transducer (4), wherein the upper end setting of the transducer (4) is provided with test tube and determines
Position hole, is provided with the spring plenum length for spring installation between the lower mounting blocks (96) and inner sleeve canister portion (97) of the cup lid group (92)
(99), outer sleeve portion (95) upper end of the installed part (91) is provided with flange (90).
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CN201510214710.9A CN104877904B (en) | 2015-04-30 | 2015-04-30 | A kind of bacterium disperses calculating instrument |
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CN201510214710.9A CN104877904B (en) | 2015-04-30 | 2015-04-30 | A kind of bacterium disperses calculating instrument |
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CN104877904A CN104877904A (en) | 2015-09-02 |
CN104877904B true CN104877904B (en) | 2018-05-15 |
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Families Citing this family (2)
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CN109385372B (en) * | 2018-09-20 | 2021-07-13 | 宁波新芝生物科技股份有限公司 | Bacterium ultrasonic dispersion counter |
CN112426943A (en) * | 2020-11-30 | 2021-03-02 | 深圳达远辰光科技有限公司 | Method for dispersing microorganisms by adopting focused ultrasound and sample processing system |
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CN2472225Y (en) * | 2001-04-05 | 2002-01-16 | 张海平 | Online lurbidimeter inspecter |
CN2514351Y (en) * | 2001-12-20 | 2002-10-02 | 孙墨杰 | Batch-type on-line automatic washing turbidimeter |
US20060042671A1 (en) * | 2003-10-24 | 2006-03-02 | Connelly Rowan T | Ultrasonic optical cleaning system |
CN104190488B (en) * | 2014-08-13 | 2015-09-16 | 广东体必康生物科技有限公司 | A kind of sealing anti-return test tube cap |
CN104263718B (en) * | 2014-09-26 | 2017-11-24 | 广东体必康生物科技有限公司 | A kind of ultrasonic wave microorganism process for dispersing and device |
CN204074124U (en) * | 2014-10-14 | 2015-01-07 | 焦伟 | A kind of clinical laboratory rack for test tube |
CN204779606U (en) * | 2015-04-30 | 2015-11-18 | 广东体必康生物科技有限公司 | Bacterium dispersion calculating instrument |
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Address after: 528300 room 401-421, floor 1-3, building a, Foshan China Europe service center, No. 2, South Lingnan Avenue, xingle community, Lecong Town, Shunde District, Foshan City, Guangdong Province Patentee after: Tibikon Biotechnology (Guangdong) Co.,Ltd. Address before: 528000 1 Jiqing Road, Foshan new town, Foshan, Guangdong Patentee before: TB Healthcare Co.,Ltd. |
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