CN109385372B - Bacterium ultrasonic dispersion counter - Google Patents
Bacterium ultrasonic dispersion counter Download PDFInfo
- Publication number
- CN109385372B CN109385372B CN201811098925.9A CN201811098925A CN109385372B CN 109385372 B CN109385372 B CN 109385372B CN 201811098925 A CN201811098925 A CN 201811098925A CN 109385372 B CN109385372 B CN 109385372B
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- ultrasonic
- hammer
- ultrasonic wave
- unthreaded hole
- microswitch
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
Abstract
The invention discloses a bacteria ultrasonic dispersion counter, wherein a shell is provided with a sample inlet, a detachable light shield is arranged at the sample inlet, a detection chamber and an ultrasonic hammer are arranged in the shell, piezoelectric ceramic transducer and actuating mechanism, the logical unthreaded hole that link up around having seted up on the detection room, the front side of the one end of logical unthreaded hole is provided with the light source, the front side of the other end of logical unthreaded hole is provided with the sensor, the ultrasonic wave hits the hammer and sets up the one side at the detection room, one side lateral wall of detection room is provided with the trompil that supplies the ultrasonic wave hammer business turn over, the central line perpendicular to logical unthreaded hole's of ultrasonic wave hammer central line, piezoelectric ceramic transducer fixed connection is at the rear end of ultrasonic wave hammer, piezoelectric ceramic transducer is connected with the high frequency power electricity, ultrasonic wave sound energy passes through the ultrasonic wave hammer transmission to the cell colorimetric ware, actuating mechanism is used for driving piezoelectric ceramic transducer reciprocating linear motion all around. The bacteria ultrasonic dispersion counter is safe and reliable, has high dispersion efficiency, and can realize high-precision McLeod turbidity measurement.
Description
Technical Field
The invention relates to a microorganism scientific research instrument, in particular to a bacteria ultrasonic dispersion counter.
Background
In the field of microbial research, the preparation of a uniform dispersion bacterial suspension meeting requirements is a basic operation, but for easily agglomerated bacteria, especially pathogenic bacteria such as mycobacterium tuberculosis, the existing treatment method has many disadvantages, such as manual grinding by using a bacterium grinding bottle, the method has poor dispersion effect and is difficult to quantify and repeat, the operation is carried out in an open container, microbial pollution is easily caused in the grinding and diluting processes, and operators are easily infected, so that the health of the operators is threatened.
In addition, in the preparation process of the bacterial suspension, the bacterial suspension with specific Mycoplasma turbidity is often required to be prepared, the conventional method is to pick the lawn into a diluent, measure the Mycoplasma turbidity after vortex oscillation, and obtain the required turbidity bacterial suspension by adjusting the diluent or the lawn amount for multiple times, so the operation process is complicated and labor-consuming, and the pathogenic bacteria are easy to cause microbial pollution.
Disclosure of Invention
The invention aims to solve the technical problem of providing a safe and reliable bacterial ultrasonic dispersion counter with high dispersion efficiency aiming at the defects of the prior art.
The technical scheme adopted by the invention for solving the technical problems is as follows: the utility model provides a bacterium supersound dispersion count appearance, includes the shell, the shell on be provided with and be used for inserting the introduction port of establishing the cell, introduction port department be provided with the detachable lens hood, the shell in be provided with detection room, ultrasonic wave hammer, piezoceramics transducer and actuating mechanism, the detection room be located the below of introduction port, the detection room on set up the light hole that link up around, the front side of one end of light hole be provided with towards the light source of light hole, the front side of the other end of light hole be provided with towards the sensor of light hole, the ultrasonic wave hammer set up one side of detection room, one side lateral wall of detection room be provided with the trompil that supplies the ultrasonic wave hammer business turn over, the central line of ultrasonic wave hammer perpendicular to the central line of light hole, piezoelectric ceramic transducer fixed connection be in the rear end of ultrasonic wave drive hammer, piezoelectric ceramic transducer be connected with the high frequency power electricity, piezoelectric ceramic transducer be used for turning into ultrasonic sound energy with high frequency electric energy, ultrasonic sound energy pass through ultrasonic wave drive hammer transmit the cell, actuating mechanism be used for driving piezoelectric ceramic transducer reciprocating linear motion around, thereby make ultrasonic wave drive hammer and cell contact or contactless.
According to the bacteria ultrasonic dispersion counter, high-frequency electric energy is converted into ultrasonic sound energy through the piezoelectric ceramic transducer, the ultrasonic sound energy is transmitted to the cuvette through the ultrasonic hammer, the ultrasonic uniform dispersion of clustered bacteria in the cuvette is realized on the premise of not damaging a sample, the operation process is quantifiable and repeatable, the dispersion efficiency is high, the dispersion effect is standardized, and the rapid colony counting can be realized. In addition, the light that the light source produced shines on the sensor through the cell, reads the intensity of transmitted light through the sensor, detects the McLeod turbidity of sample in view of the above, realizes the McLeod turbidity measurement of high accuracy. The dispersion and detection of the thalli are carried out in a sealed environment, the safety and the reliability are realized, no microbial pollution is generated,
before the bacteria ultrasonic dispersion counter is used, a cuvette packaged with bacteria is inserted into a sample inlet, a piezoelectric ceramic transducer and an ultrasonic hammer are driven by a driving mechanism to move towards the cuvette together, the ultrasonic hammer is tightly attached to the outer wall of the cuvette, and then the piezoelectric ceramic transducer, a light source and a sensor are electrified to start dispersion of the bacteria. The ultrasonic hammer is only tightly attached to the outer wall of the cuvette in a working state, and the ultrasonic hammer is not in contact with the outer wall of the cuvette in other time, so that the influence of heating of the ultrasonic hammer on the colorimetric cuvette can be avoided, and the accuracy of a detection result is ensured.
Preferably, the driving mechanism comprises a variable speed motor, a crankshaft, a convex rod, a connecting rod and a push rod, a mounting plate is arranged at the bottom of the shell, the piezoelectric ceramic transducer is fixed on a fixing seat, the fixing seat is slidably mounted on the mounting plate, the variable speed motor is vertically mounted below the mounting plate, one end of the crankshaft is mounted at the output end of the variable speed motor, the other end of the crankshaft penetrates through the mounting plate to be connected with the convex rod, one end of the connecting rod is rotatably connected with the other end of the crankshaft, the other end of the connecting rod is rotatably connected with one end of the push rod, the other end of the push rod is fixed on the fixing seat, a first micro switch and a second micro switch which are connected in parallel are mounted on the mounting plate, and driving rods are respectively arranged on the first micro switch and the second micro switch, the first microswitch and the second microswitch are respectively arranged on two sides of the convex rod and are respectively used for controlling the start and stop of the variable speed motor. When the reciprocating linear motion mechanism works, the variable speed motor drives the crankshaft to rotate, and the ejector rod is pushed to reciprocate linearly through the connecting rod. The crankshaft is connected with a convex rod, when the crankshaft rotates and pushes the ejector rod to move towards the direction of the ultrasonic hammer, the convex rod touches a driving rod of the first microswitch, the power supply of the variable speed motor is cut off, the fixed seat stops moving, and the ultrasonic hammer is tightly attached to the outer wall of the cuvette; when the variable speed motor drives the crankshaft to rotate in the opposite direction, the ejector rod is dragged to move back, and when the convex rod touches the driving rod of the second microswitch, the power supply of the variable speed motor is cut off, the fixing seat stops moving, and the ultrasonic hammer is separated from the cuvette and enters a standby state.
Furthermore, the ejector rod is arranged on an installation seat in a penetrating mode, a spring is sleeved on the ejector rod, one end of the spring is fixed on the installation seat, and the other end of the spring is fixed on the fixed seat. The setting of spring is favorable to restricting the moving distance of fixing base and ultrasonic wave hammer towards the cell direction, avoids causing the cell pressurized damage because of the moving distance is bigger than normal.
Furthermore, the mounting plate is provided with a guide rail, and the fixed seat is slidably mounted on the guide rail. Under the drive of the driving mechanism, the fixed seat linearly moves back and forth along the guide rail to drive the piezoelectric ceramic transducer and the ultrasonic hammer to linearly move back and forth in a reciprocating manner.
Preferably, the sample inlet is a square sample inlet for inserting a square cuvette. The square sample inlet can meet the insertion requirement of a square cuvette, for example, a square cuvette such as a standard cuvette of 1 cm.
Preferably, the sensor is a light intensity sensor with an analog-to-digital conversion circuit, so as to overcome the influence of temperature fluctuation and electromagnetic interference on the detection result.
Preferably, the shell is provided with a baffle plate, the baffle plate is arranged above the detection chamber, and the sample inlet is simultaneously arranged on the shell and the baffle plate. The baffle can play further shading effect to play certain guard action to the detection room.
Preferably, the detection chamber is made of a non-metal material, so that the interference of the piezoelectric ceramic transducer on the optical system is reduced, and the accuracy of the detection result is further improved.
Compared with the prior art, the invention has the following advantages: according to the bacteria ultrasonic dispersion counter disclosed by the invention, high-frequency electric energy is converted into ultrasonic sound energy through the piezoelectric ceramic transducer, the ultrasonic sound energy is transmitted to the cuvette through the ultrasonic hammer, and the uniform dispersion of the ultrasonic of the clustered bacteria in the cuvette is realized on the premise of not damaging a sample. In addition, the light that the light source produced shines on the sensor through the cell, reads the intensity of transmitted light through the sensor, detects the McLeod turbidity of sample in view of the above, realizes the McLeod turbidity measurement of high accuracy.
Drawings
FIG. 1 is an external view of an ultrasonic bacteria dispersion counter according to an embodiment;
FIG. 2 is an illustration showing the effect of the ultrasonic hammer on the assembled detection chamber in the embodiment;
FIG. 3 is a diagram showing the effect of the ultrasonic hammer and the driving mechanism in the embodiment after they are assembled.
Detailed Description
The invention is described in further detail below with reference to the accompanying examples.
The bacteria ultrasonic dispersion counter of embodiment 1, as shown in the figure, includes a housing 1, a sample inlet 11 is provided on the housing 1, the sample inlet 11 is a square sample inlet with a diameter of 1cm, and is used for inserting a 1cm standard cuvette 6, a detachable light shield 12 is provided at the sample inlet 11, a detection chamber 2, an ultrasonic hammer 3, a piezoelectric ceramic transducer 4 and a driving mechanism are provided in the housing 1, the detection chamber 2 is located below the sample inlet 11, a light through hole 21 is provided on the detection chamber 2, a light source 22 facing the light through hole 21 is provided at a front side of one end of the light through hole 21, in this embodiment, the light source 22 is a light emitting diode, a sensor 23 facing the light through hole 21 is provided at a front side of the other end of the light through hole 21, the sensor 23 is a light intensity sensor with an analog-to-digital conversion circuit, the ultrasonic hammer 3 is provided at one side of the detection chamber 2, an opening 24 for the ultrasonic hammer 3 to enter and exit is provided at a side wall of, the central line of ultrasonic wave drive hammer 3 is perpendicular to the central line of logical unthreaded hole 21, and piezoceramics transducer 4 fixed connection is in the rear end of ultrasonic wave drive hammer 3, and piezoceramics transducer 4 is connected with high frequency power (not shown in the figure) electricity, and piezoceramics transducer 4 is used for turning into ultrasonic sound energy with high frequency electric energy, and ultrasonic sound energy passes through ultrasonic wave drive hammer 3 and transmits to cell 6, and actuating mechanism is used for driving piezoceramics transducer 4 reciprocating linear motion back and forth to make ultrasonic wave drive hammer 3 and cell 6 contact or not.
In embodiment 1, a baffle 13 is installed on the housing 1, the baffle 13 is disposed above the detection chamber 2, and the sample inlet 11 is disposed on both the housing 1 and the baffle 13; the detection chamber 2 is a detection chamber made of a non-metallic material.
The ultrasonic bacteria dispersion counter of embodiment 2 differs from embodiment 1 in that in embodiment 2, the driving mechanism includes a variable speed motor 51, a crankshaft 52, a protruding rod 53, a connecting rod 54 and a push rod 55, the bottom of the housing 1 is provided with a mounting plate 14, the piezoelectric ceramic transducer 4 is fixed on a fixed seat 41, the mounting plate 14 is provided with a guide rail 10, the fixed seat 41 is slidably mounted on the guide rail 10, the variable speed motor 51 is vertically mounted below the mounting plate 14, one end of the crankshaft 52 is mounted at the output end of the variable speed motor 51, the other end of the crankshaft 52 passes through the mounting plate 14 to be connected with the protruding rod 53, one end of the connecting rod 54 is rotatably connected with the other end of the crankshaft 52, the other end of the connecting rod 54 is rotatably connected with one end of the push rod 55, the other end of the push rod 55 is fixed on the fixed seat 41, the push rod 55 is arranged on a mounting seat 56 in a penetrating manner, the other end of spring 57 is fixed on fixing base 41, installs parallelly connected first micro-gap switch 15 and second micro-gap switch 16 on the mounting panel 14, is provided with actuating lever 17 and 18 on first micro-gap switch 15 and the second micro-gap switch 16 respectively, and first micro-gap switch 15 and second micro-gap switch 16 set up the both sides at protruding pole 53 respectively, and first micro-gap switch 15 and second micro-gap switch 16 are used for controlling the opening and stop of gear motor 51 respectively. During operation, the variable speed motor 51 drives the crankshaft 52 to rotate, and the push rod 55 is pushed by the connecting rod 54 to reciprocate linearly. The crankshaft 52 is connected with a convex rod 53, when the crankshaft 52 rotates and pushes the ejector rod 55 to move towards the direction of the ultrasonic hammer 3, the convex rod 53 touches the driving rod 17 of the first microswitch 15, namely the power supply of the variable speed motor 51 is cut off, the fixed seat 41 stops moving, and the ultrasonic hammer 3 is tightly attached to the outer wall of the cuvette 6; when the variable speed motor 51 drives the crankshaft 52 to rotate in the opposite direction, the ejector rod 55 is dragged to move back, and when the protruding rod 53 touches the driving rod 18 of the second microswitch 16, the power supply of the variable speed motor 51 is cut off, the fixed seat 41 stops moving, and the ultrasonic hammer 3 is separated from the cuvette 6 and enters a standby state.
Before the bacteria ultrasonic dispersion counter is used, a cuvette 6 packaged with bacteria is inserted into a sample inlet 11, a driving mechanism drives a piezoelectric ceramic transducer 4 and an ultrasonic hammer 3 to move towards the cuvette 6 together, the ultrasonic hammer 3 is enabled to be tightly attached to the outer wall of the cuvette 6, then the piezoelectric ceramic transducer 4, a light source 22 and a sensor 23 are electrified, the bacteria can be dispersed, high-frequency electric energy is converted into ultrasonic sound energy through the piezoelectric ceramic transducer 4, the ultrasonic sound energy is transmitted to the cuvette 6 through the ultrasonic hammer 3, and the uniform dispersion of the ultrasonic of the bacteria in the cuvette 6 is realized on the premise of not damaging a sample. The light generated by the light source 22 passes through the cuvette 6 and shines on the sensor 23, and the intensity of the transmitted light is read by the sensor 23, so that the McLeod turbidity of the sample is detected, and the high-precision McLeod turbidity measurement is realized. The ultrasonic hammer 3 is only clung to the outer wall of the cuvette 6 in a working state, and is not contacted with the outer wall of the cuvette 6 in other time, so that the influence of the heating of the ultrasonic hammer 3 on the cuvette 6 can be avoided, and the accuracy of a detection result is ensured.
Claims (5)
1. The utility model provides a bacterium supersound dispersion count appearance, includes the shell, the shell on be provided with and be used for inserting the introduction port of establishing the cell, introduction port department be provided with detachable lens hood, its characterized in that: the shell in be provided with detection room, ultrasonic wave drive hammer, piezoceramics transducer and actuating mechanism, the detection room be located the below of introduction port, the detection room on set up the logical unthreaded hole that link up around, the front side of the one end of logical unthreaded hole be provided with the orientation the light source of logical unthreaded hole, the front side of the other end of logical unthreaded hole be provided with the orientation the sensor of logical unthreaded hole, the ultrasonic wave drive hammer set up one side of detection room, one side lateral wall of detection room be provided with the trompil that supplies the ultrasonic wave drive hammer business turn over, the central line of ultrasonic wave drive hammer perpendicular to the central line of logical unthreaded hole, piezoceramics transducer fixed connection in the rear end of ultrasonic wave drive hammer, piezoceramics transducer be connected with the high frequency power electricity, piezoceramics transducer be used for turning into ultrasonic acoustic energy with high frequency electric energy, ultrasonic sound energy pass through ultrasonic hammer transmit the cell, actuating mechanism be used for the drive piezoceramics transducer reciprocating linear motion back and forth to make ultrasonic hammer and cell contact or contactless, actuating mechanism include variable speed motor, bent axle, nose bar, connecting rod and ejector pin, the bottom of shell be provided with the mounting panel, piezoceramics transducer fix on a fixing base, the mounting panel on install the guide rail, fixing base slidable install on the guide rail, variable speed motor install upright the below of mounting panel, the one end of bent axle install the output of variable speed motor, the other end of bent axle pass the mounting panel with the nose bar be connected, the one end of connecting rod with the other end of bent axle rotate and be connected, the other end of the connecting rod is rotatably connected with one end of the ejector rod, the other end of the ejector rod is fixed on the fixed seat, a first microswitch and a second microswitch which are connected in parallel are installed on the mounting plate, driving rods are respectively arranged on the first microswitch and the second microswitch, the first microswitch and the second microswitch are respectively arranged on two sides of the convex rod, and the first microswitch and the second microswitch are respectively used for controlling the start and stop of the variable speed motor; the sample inlet is a square sample inlet and is used for inserting a square cuvette.
2. The bacteria ultrasonic dispersion counter of claim 1, wherein: the ejector rod is arranged on a mounting seat in a penetrating mode, a spring is sleeved on the ejector rod, one end of the spring is fixed on the mounting seat, and the other end of the spring is fixed on the fixing seat.
3. The bacteria ultrasonic dispersion counter of claim 1, wherein: the sensor is a light intensity sensor with an analog-to-digital conversion circuit.
4. The bacteria ultrasonic dispersion counter of claim 1, wherein: the shell on install the baffle, the baffle setting be in the top of detection room, the introduction port set up simultaneously the shell with the baffle on.
5. The bacteria ultrasonic dispersion counter of claim 1, wherein: the detection chamber is made of non-metal materials.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811098925.9A CN109385372B (en) | 2018-09-20 | 2018-09-20 | Bacterium ultrasonic dispersion counter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811098925.9A CN109385372B (en) | 2018-09-20 | 2018-09-20 | Bacterium ultrasonic dispersion counter |
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| Publication Number | Publication Date |
|---|---|
| CN109385372A CN109385372A (en) | 2019-02-26 |
| CN109385372B true CN109385372B (en) | 2021-07-13 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811098925.9A Active CN109385372B (en) | 2018-09-20 | 2018-09-20 | Bacterium ultrasonic dispersion counter |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4495149A (en) * | 1981-09-18 | 1985-01-22 | Toa Medical Electronic Co., Ltd. | Optical-type automatic analyzing and measuring apparatus |
| CN101344468A (en) * | 2008-08-25 | 2009-01-14 | 浙江工业大学 | Sound suspending polissoir for nanometer mechanics characterization sample |
| CN101405881A (en) * | 2005-08-19 | 2009-04-08 | U.I.T.有限责任公司 | Oscillating system and tool for ultrasonic impact treatment |
| CN203422265U (en) * | 2013-07-11 | 2014-02-05 | 天津海迈医用科技有限公司 | Blood sampling mixer |
| CN204469632U (en) * | 2015-01-29 | 2015-07-15 | 北京科技大学 | A kind of composite ultraphonic even-dispersing device |
| CN104877904A (en) * | 2015-04-30 | 2015-09-02 | 广东体必康生物科技有限公司 | Bacterial dispersion counter |
| CN107843583A (en) * | 2017-10-16 | 2018-03-27 | 南京岚煜生物科技有限公司 | A kind of micro-fluidic chemiluminescent analyzer and application method |
| US10472621B2 (en) * | 2013-11-01 | 2019-11-12 | Leadway (Hk) Limited | Nucleic acid extraction apparatus |
-
2018
- 2018-09-20 CN CN201811098925.9A patent/CN109385372B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4495149A (en) * | 1981-09-18 | 1985-01-22 | Toa Medical Electronic Co., Ltd. | Optical-type automatic analyzing and measuring apparatus |
| CN101405881A (en) * | 2005-08-19 | 2009-04-08 | U.I.T.有限责任公司 | Oscillating system and tool for ultrasonic impact treatment |
| CN101344468A (en) * | 2008-08-25 | 2009-01-14 | 浙江工业大学 | Sound suspending polissoir for nanometer mechanics characterization sample |
| CN203422265U (en) * | 2013-07-11 | 2014-02-05 | 天津海迈医用科技有限公司 | Blood sampling mixer |
| US10472621B2 (en) * | 2013-11-01 | 2019-11-12 | Leadway (Hk) Limited | Nucleic acid extraction apparatus |
| CN204469632U (en) * | 2015-01-29 | 2015-07-15 | 北京科技大学 | A kind of composite ultraphonic even-dispersing device |
| CN104877904A (en) * | 2015-04-30 | 2015-09-02 | 广东体必康生物科技有限公司 | Bacterial dispersion counter |
| CN107843583A (en) * | 2017-10-16 | 2018-03-27 | 南京岚煜生物科技有限公司 | A kind of micro-fluidic chemiluminescent analyzer and application method |
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| CN109385372A (en) | 2019-02-26 |
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