CN104872766A - Full-nutrient beef bone protein beverage and preparation method - Google Patents
Full-nutrient beef bone protein beverage and preparation method Download PDFInfo
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- CN104872766A CN104872766A CN201510274025.5A CN201510274025A CN104872766A CN 104872766 A CN104872766 A CN 104872766A CN 201510274025 A CN201510274025 A CN 201510274025A CN 104872766 A CN104872766 A CN 104872766A
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- bone
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- enzymolysis
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- bone protein
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- 210000000988 bone and bone Anatomy 0.000 title claims abstract description 91
- 235000021568 protein beverage Nutrition 0.000 title claims abstract description 11
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 235000015278 beef Nutrition 0.000 title abstract 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 33
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 24
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 24
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 230000002255 enzymatic effect Effects 0.000 claims abstract description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000003381 stabilizer Substances 0.000 claims abstract description 7
- 238000000855 fermentation Methods 0.000 claims abstract description 6
- 230000004151 fermentation Effects 0.000 claims abstract description 6
- 238000010438 heat treatment Methods 0.000 claims abstract description 5
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 4
- 102100030684 Sphingosine-1-phosphate phosphatase 1 Human genes 0.000 claims description 12
- 101710168942 Sphingosine-1-phosphate phosphatase 1 Proteins 0.000 claims description 12
- 235000016709 nutrition Nutrition 0.000 claims description 11
- 230000035764 nutrition Effects 0.000 claims description 10
- 239000002245 particle Substances 0.000 claims description 9
- 230000004913 activation Effects 0.000 claims description 7
- 239000012530 fluid Substances 0.000 claims description 7
- 102000057297 Pepsin A Human genes 0.000 claims description 6
- 108090000284 Pepsin A Proteins 0.000 claims description 6
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 6
- 239000011707 mineral Substances 0.000 claims description 6
- 229940111202 pepsin Drugs 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 238000009835 boiling Methods 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 3
- 229940088598 enzyme Drugs 0.000 claims description 3
- 239000012467 final product Substances 0.000 claims description 3
- 239000004519 grease Substances 0.000 claims description 3
- 238000000227 grinding Methods 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 238000009928 pasteurization Methods 0.000 claims description 3
- 238000010298 pulverizing process Methods 0.000 claims description 3
- 238000005238 degreasing Methods 0.000 abstract description 8
- 239000000047 product Substances 0.000 abstract description 5
- 239000000796 flavoring agent Substances 0.000 abstract description 4
- 235000019634 flavors Nutrition 0.000 abstract description 4
- 238000000498 ball milling Methods 0.000 abstract description 2
- 235000013361 beverage Nutrition 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 abstract description 2
- 235000015097 nutrients Nutrition 0.000 abstract description 2
- 239000002904 solvent Substances 0.000 abstract description 2
- 102000004142 Trypsin Human genes 0.000 abstract 2
- 108090000631 Trypsin Proteins 0.000 abstract 2
- 239000012588 trypsin Substances 0.000 abstract 2
- 239000000413 hydrolysate Substances 0.000 abstract 1
- 230000000717 retained effect Effects 0.000 abstract 1
- 244000144972 livestock Species 0.000 description 5
- 235000013594 poultry meat Nutrition 0.000 description 5
- 244000144977 poultry Species 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 3
- 235000013372 meat Nutrition 0.000 description 3
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000002893 slag Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 208000019901 Anxiety disease Diseases 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 229920002567 Chondroitin Polymers 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- 102000007982 Phosphoproteins Human genes 0.000 description 1
- 108010089430 Phosphoproteins Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 230000036506 anxiety Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- DLGJWSVWTWEWBJ-HGGSSLSASA-N chondroitin Chemical compound CC(O)=N[C@@H]1[C@H](O)O[C@H](CO)[C@H](O)[C@@H]1OC1[C@H](O)[C@H](O)C=C(C(O)=O)O1 DLGJWSVWTWEWBJ-HGGSSLSASA-N 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000003908 liver function Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000000192 social effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a full-nutrient beef bone protein beverage which is characterized by comprising the following components in parts by weight: 80-100 parts of fresh beef bone, 2-3 parts of n-hexane, 8-10 parts of ethanol, 1-2 parts of citric acid, 10 parts of pepsase, 10-20 parts of trypsin, 3-5 parts of saccharomycetes, 0.5-0.7 part of a stabilizer and a proper amount of water. According to the invention, degreasing and enzymolysis of beef bone are realized through stewing of the solvent n-hexane by soft fire at low pressure, enzymolysis of trypsin and assisted enzymolysis of citric acid and pepsase, sufficient degreasing is ensured through double-degreasing, further degreasing is realized through double enzymolysis, sufficient bone protein enzymolysis is ensured, the flavor of an enzymatic bone protein product is controlled in combination with the citric acid, the generation of the flavor of the enzymatic bone protein product is facilitated through fermentation, finally, rest bone residues are subjected to microwave heating to be calcined, activated by ethanol, and subjected to ball milling, and fermentation liquid is added for preparing the beverage; since bone protein is used and combined with enzymatic hydrolysate and final residual bone residues in the preparation process, full nutrients of the beef bone are retained after unpleasant smell of the beef bone is removed as much as possible.
Description
Technical field
The invention belongs to ox bone field of deep, be specifically related to a kind of full nutrition ox bone protein beverage and preparation method thereof.
Background technology
China is the maximum country of cowboying in the world, and be the first in the world production of meat big country, annual China about has the livestock and poultry bone of nearly 1,000 ten thousand tons to produce, and bone resource is very abundant.In the chemical composition of bone, moisture accounts for 40 % ~ 50%, collagen accounts for 20% ~ 30%, inanimate matter accounts for 20% (mainly calcium and phosphorus).Osteotrophy enriches, and the content of protein, fat is similar to equivalent fresh meat, and the mineral elements such as C a, P, F e, Z n are the several times of fresh meat, and ratio is suitable for.SPP1 is the soluble protein of comparatively full price, and biological value is high.In addition, also have the indispensable Phospholipids of human brain, phosphoprotein, prevent old and feeble chondroitin, ossein, the various nutrients such as the methionine of liver function can be promoted.SPP1 food also can be used for cosmetic health product, meets the nutritional need of the special population such as sportsman of children in growth period, convalescent, the elderly and high physical consumption.The protein of bone containing 11 % ~ 15 % itself, and be a kind of protein resource of high-quality.The protein resource of China is always more nervous, and the exploitation of SPP1 resource are significant to the protein resource anxiety alleviating China.China is a livestock and poultry consumption big country, but only concentrates on the consumption of livestock and poultry meat, and a large amount of livestock and poultry bones is not used, both waste resource, polluted environment again, bring certain environmental pressure, therefore, the comprehensive utilization of livestock and poultry bone is the research direction of numerous researcher always.Develop ox bone resource, in order to production high-purity SPP1, both can make full use of ox bone resource, turn waste into wealth, reduce environmental pollution, solve raw ox processing industry comprehensive utilization of resources problem, the raw material sources of edible protein can be expanded again simultaneously, there is great social effect and economic worth.
Summary of the invention
The present invention is directed to the demand of ox bone comprehensive development and utilization, propose a kind of full nutrition ox bone protein beverage and preparation method thereof.
The technical solution used in the present invention is as follows:
A kind of full nutrition ox bone protein beverage, it is characterized in that, comprise following parts by weight of component: fresh ox bone 80-100, n-hexane 2-3, ethanol 8-10, citric acid 1-2, pepsin 8-10, trypsase 10-20, saccharomycete 3-5, stabilizing agent 0.5-0.7 and appropriate water.
A kind of full nutrition ox bone protein beverage preparation method, is characterized in that, comprise the following steps:
(1) get fresh ox bone to wash with water totally, put into disintegrating machine to be crushed to 2-3cm and to look ox bone section, put into pressure cooker afterwards, after adding gross weight 2-3 water doubly, add n-hexane, under low pressure 2-3KPa effect, little fiery boiling 3-4h, after cooling, be separated ox bone section, Galbitang and ox bone grease for subsequent use;
(2) get ox bone section to put into vacuum microwave put into pulverizer after the dry 30-40min of 100W microwave, crossing 100 orders after pulverizing, to obtain ox bone particle for subsequent use; After adding ox bone particle, trypsase and gross weight 5-6 water doubly in Galbitang, rapid stirring enzymolysis 20-30min at 40-50 DEG C, filter afterwards ox bone protein enzymatic hydrolyzate one and ox bone residue one for subsequent use;
(3) will add citric acid and pepsin and gross weight 2-3 water doubly in ox bone residue one, at 30-40 DEG C, slowly stir enzymolysis 30-40mi, hyperfiltration afterwards obtains ox bone protein enzymatic hydrolyzate two and ox bone residue two;
(4) ox bone protein enzymatic hydrolyzate one and ox bone protein enzymatic hydrolyzate two are mixed, normal temperature lucifuge fermentation 3-5d after inoculation yeast bacterium wherein after the 80-90 DEG C of enzyme 20-30min that goes out, obtains SPP1 zymotic fluid for subsequent use;
(5) get after ox bone residue two adopts 1000W heating using microwave 10-20min in vacuum microwave, put into ethanolic solution heat-activated 3-4min at 120 DEG C, putting into ball mill ultramicro grinding afterwards, to be that particle diameter 90um obtains bone activation mineral powder for subsequent use;
(6) get SPP1 zymotic fluid and bone activation mineral powder and stabilizing agent mixing homogeneous after, after pasteurization fill fill with and get final product.
Beneficial effect of the present invention is embodied in:
The present invention passes through solvent hexane, the little fiery boiling of low pressure, and tryptic enzymolysis, citric acid and pepsic assistance enzymolysis realize degreasing and the enzymolysis of ox bone, two degreasing ensure that fully carrying out of degreasing, the further degreasing of double enzymolysis, and ensure that fully carrying out of SPP1 enzymolysis, combining citric acid controls the local flavor of enzymatic hydrolysis of bones product, and facilitate the generation of the local flavor of enzymolysis protein product by fermentation, remaining bone slag is calcined through heating using microwave the most at last, Ethanol activation, zymotic fluid is added after ball milling, prepare beverage, due in manufacturing process, not only make use of SPP1, in conjunction with enzymolysis liquid and final residue bone slag, whole nutrition of ox bone are left after bad smell removing ox bone as much as possible.
Detailed description of the invention
Embodiment
Full nutrition ox bone protein beverage described in the present embodiment, is characterized in that, comprise following parts by weight of component: fresh ox bone 90, n-hexane 2.5, ethanol 9, citric acid 1.5, pepsin 9, trypsase 15, saccharomycete 4, stabilizing agent 0.6 and appropriate water.
Full nutrition ox bone protein beverage preparation method described in the present embodiment, is characterized in that, comprise the following steps:
(1) get fresh ox bone to wash with water totally, put into disintegrating machine to be crushed to 2-3cm and to look ox bone section, put into pressure cooker afterwards, after adding gross weight 2-3 water doubly, add n-hexane, under low pressure 2-3KPa effect, little fiery boiling 3-4h, after cooling, be separated ox bone section, Galbitang and ox bone grease for subsequent use;
(2) get ox bone section and put into vacuum microwave at the dry 30-40min of 100W microwave, put into pulverizer afterwards, it is for subsequent use that rear 100 orders excessively of pulverizing obtain ox bone particle; After adding ox bone particle, trypsase and gross weight 5-6 water doubly in Galbitang, rapid stirring enzymolysis 20-30min at 40-50 DEG C, filter afterwards ox bone protein enzymatic hydrolyzate one and ox bone residue one for subsequent use;
(3) will add citric acid and pepsin and gross weight 2-3 water doubly in ox bone residue one, at 30-40 DEG C, slowly stir enzymolysis 30-40mi, hyperfiltration afterwards obtains ox bone protein enzymatic hydrolyzate two and ox bone residue two;
(4) ox bone protein enzymatic hydrolyzate one and ox bone protein enzymatic hydrolyzate two are mixed, normal temperature lucifuge fermentation 3-5d after inoculation yeast bacterium wherein after the 80-90 DEG C of enzyme 20-30min that goes out, obtains SPP1 zymotic fluid for subsequent use;
(5) get after ox bone residue two adopts 1000W heating using microwave 10-20min in vacuum microwave, put into ethanolic solution heat-activated 3-4min at 120 DEG C, putting into ball mill ultramicro grinding afterwards, to be that particle diameter 90um obtains bone activation mineral powder for subsequent use;
(6) get SPP1 zymotic fluid and bone activation mineral powder and stabilizing agent mixing homogeneous after, after pasteurization fill fill with and get final product.
Claims (2)
1. a full nutrition ox bone protein beverage, it is characterized in that, comprise following parts by weight of component: fresh ox bone 80-100, n-hexane 2-3, ethanol 8-10, citric acid 1-2, pepsin 8-10, trypsase 10-20, saccharomycete 3-5, stabilizing agent 0.5-0.7 and appropriate water.
2. a full nutrition ox bone protein beverage preparation method, is characterized in that, comprise the following steps:
(1) get fresh ox bone to wash with water totally, put into disintegrating machine to be crushed to 2-3cm and to look ox bone section, put into pressure cooker afterwards, after adding gross weight 2-3 water doubly, add n-hexane, under low pressure 2-3KPa effect, little fiery boiling 3-4h, after cooling, be separated ox bone section, Galbitang and ox bone grease are for subsequent use;
(2) get ox bone section and put into vacuum microwave at the dry 30-40min of 100W microwave, put into pulverizer afterwards, it is for subsequent use that rear 100 orders excessively of pulverizing obtain ox bone particle; After adding ox bone particle, trypsase and gross weight 5-6 water doubly in Galbitang, rapid stirring enzymolysis 20-30min at 40-50 DEG C, filter afterwards ox bone protein enzymatic hydrolyzate one and ox bone residue one for subsequent use;
(3) will add citric acid and pepsin and gross weight 2-3 water doubly in ox bone residue one, at 30-40 DEG C, slowly stir enzymolysis 30-40mi, hyperfiltration afterwards obtains ox bone protein enzymatic hydrolyzate two and ox bone residue two;
(4) ox bone protein enzymatic hydrolyzate one and ox bone protein enzymatic hydrolyzate two are mixed, normal temperature lucifuge fermentation 3-5d after inoculation yeast bacterium wherein after the 80-90 DEG C of enzyme 20-30min that goes out, obtains SPP1 zymotic fluid for subsequent use;
(5) get after ox bone residue two adopts 1000W heating using microwave 10-20min in vacuum microwave, put into ethanolic solution heat-activated 3-4min at 120 DEG C, putting into ball mill ultramicro grinding afterwards, to be that particle diameter 90um obtains bone activation mineral powder for subsequent use;
(6) get SPP1 zymotic fluid and bone activation mineral powder and stabilizing agent mixing homogeneous after, after pasteurization fill fill with and get final product.
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CN201510274025.5A CN104872766A (en) | 2015-05-27 | 2015-05-27 | Full-nutrient beef bone protein beverage and preparation method |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110214954A (en) * | 2019-07-16 | 2019-09-10 | 江西师范大学 | A kind of fish-bone calcium peptide lotion and preparation method thereof rich in DHA |
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Application publication date: 20150902 |