CN104855286A - Tongling peony tissue culture and rapid propagation seedling raising technical method - Google Patents
Tongling peony tissue culture and rapid propagation seedling raising technical method Download PDFInfo
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- CN104855286A CN104855286A CN201510214232.1A CN201510214232A CN104855286A CN 104855286 A CN104855286 A CN 104855286A CN 201510214232 A CN201510214232 A CN 201510214232A CN 104855286 A CN104855286 A CN 104855286A
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Abstract
The invention discloses a Tongling peony tissue culture and rapid propagation seedling raising technical method. The Tongling peony tissue culture and rapid propagation seedling raising technical method comprises the following steps of explant selection which comprises selecting Tongling peony buds as an explant; explant disinfection which comprises stripping scales after the explant is washed through the flowing water until the explant is clear, performing 30 seconds of soaking through 70% of volume fraction of alcohol, performing 1 minute of sterilization through 0.1% of concentration of mercuric chloride and performing three times of rinsing through sterile water, wherein every time of rinsing lasts 1 minute; culture medium induction through cluster buds, wherein the formula is as follows and the culture period is 15 days; culture medium rooting, wherein the formula is as follows and the culture period is 10 to 15 days; acclimatization and transplanting which comprise appropriately opening a culture bottle cap for the gas exchange inside and outside a bottle, completely opening the culture bottle cap after one day, taking out seedlings after two days of acclimatization, washing a culture medium on the seedling base portion and root in the warm water, transplanting the seedlings into a greenhouse and covering a plastic film for moisturizing, wherein 400 times of carbendazim treatment is performed on matrixes before transplanting, 800 times of carbendazim is sprayed once every week after transplanting, the temperature is 20 plus or minus 2 DEG C, and the humidity is 85%; culture period settings which comprise setting the first time of culture period as a month and shortening the culture period into half a month after a tissue culture system is built.
Description
Technical field
The present invention relates to biological husbandry technical method, especially relate to a kind of phoenix red tissue-culturing rapid propagation seedling growing process method.
Background technology
Tissue culture technique work organ, tissue or cell is placed in medium under sterile conditions, and be placed in adapt circumstance, carries out the cell of continuous culture, tissue or individuality, and this technology has been widely used in agricultural and biological, medical research.But the technique, the culture medium prescription that fast breeding technique are wherein used for the red nursery production of phoenix not yet find, this is again in demand in actual applications.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, for the red tissue culture of Fast-propagation phoenix provides phoenix red tissue-culturing rapid propagation seedling growing process method.
The present invention is achieved through the following technical measures, and a kind of phoenix red tissue-culturing rapid propagation seedling growing process method, comprises the steps:
(1) explant is selected: the red bud of phoenix;
(2) explant sterilization: rear running water peels off scale after clear, then is 70% alcohol-pickled time 30s by volume fraction, is the mercuric chloride sterilization time 1min of 0.1% afterwards, finally uses rinsed with sterile water three times again, each time 1min by concentration; Above agent combination and concentration are the best sterilization methods that the red bud of phoenix obtains through orthogonal experiment;
(3) inducing clumping bud medium: 1/2MS+BA1.0mg/L+GA30.5mg/L+NAA0.1mg/L; Culture period 15 days; Above agent combination and concentration are for the red bud of phoenix, the optimum formula obtained by orthogonal experiment, and the effect of induced bud is best in test, and incubation time is the shortest;
(4) root media: 1/2MS+BA0.5mg/L+NAA0.5mg/L; Culture period ten to ten five days; Above agent combination and concentration are for the red bud of phoenix, the optimum formula obtained by orthogonal experiment, and induce in test the effect of root best, incubation time is the shortest, and test is around here carried out in blake bottle;
(5) acclimatization and transplants: the lid suitably opening blake bottle, be convenient to the gas exchanges inside and outside blake bottle, after one day, open the lid of blake bottle completely, practice seedling again after two days, seedling to be taken out, in warm water, wash away the medium on seedling base portion and root, then transplanted in booth, cover plastic film moisturizing; Before transplanting, matrix is all through 400 times of carbendazim process, after transplanting, sprays 800 times of carbendazim once weekly, temperature (20 ± 2) DEG C, humidity 85%;
(6) cultivation cycle is one month for the first time, and after group training Establishing, cycle time is two weeks.
Beneficial effect of the present invention is: the technology in prior art being all universality, at present not for technique, the culture medium prescription of the red group culturation rapid propagating technology of phoenix, the present invention is new achievement in research on technique, culture medium prescription, and being applied in of this fast breeding technique also promotes the cycle that Feng Dan group is trained greatly while not affecting group training effect.
Embodiment
A kind of phoenix red tissue-culturing rapid propagation seedling growing process method, comprises the steps:
(1) explant is selected: the red bud of phoenix;
(2) explant sterilization: rear running water peels off scale after clear, then is 70% alcohol-pickled time 30s by volume fraction, is the mercuric chloride sterilization time 1min of 0.1% afterwards, finally uses rinsed with sterile water three times again, each time 1min by concentration;
(3) inducing clumping bud medium: 1/2MS+BA1.0mg/L+GA30.5mg/L+NAA0.1mg/L; Culture period 15 days;
(4) root media: 1/2MS+BA0.5mg/L+NAA0.5mg/L; Culture period ten to ten five days;
(5) acclimatization and transplants: the lid suitably opening blake bottle, be convenient to the gas exchanges inside and outside blake bottle, after one day, open the lid of blake bottle completely, practice seedling again after two days, seedling to be taken out, in warm water, wash away the medium on seedling base portion and root, then transplanted in booth, cover plastic film moisturizing; Before transplanting, matrix is all through 400 times of carbendazim process, after transplanting, sprays 800 times of carbendazim once weekly, temperature (20 ± 2) DEG C, humidity 85%;
(6) cultivation cycle is one month for the first time, and after group training Establishing, cycle time is two weeks.
More than that a kind of phoenix of the present invention red tissue-culturing rapid propagation seedling growing process method is set forth; the present invention is understood for helping; but embodiments of the present invention are not restricted to the described embodiments; any do not deviate from the principle of the invention under do change, modification, substitute, combination, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (1)
1. a phoenix red tissue-culturing rapid propagation seedling growing process method, is characterized in that comprising the steps:
(1) explant is selected: the red bud of phoenix;
(2) explant sterilization: rear running water peels off scale after clear, then is 70% alcohol-pickled time 30s by volume fraction, is the mercuric chloride sterilization time 1min of 0.1% afterwards, finally uses rinsed with sterile water three times again, each time 1min by concentration;
(3) inducing clumping bud medium: 1/2MS+BA1.0mg/L+GA30.5mg/L+NAA0.1mg/L; Culture period 15 days;
(4) root media: 1/2MS+BA0.5mg/L+NAA0.5mg/L; Culture period ten to ten five days, test is around here carried out in blake bottle;
(5) acclimatization and transplants: the lid suitably opening blake bottle, be convenient to the gas exchanges inside and outside blake bottle, after one day, open the lid of blake bottle completely, practice seedling again after two days, seedling to be taken out, in warm water, wash away the medium on seedling base portion and root, then transplanted in booth, cover plastic film moisturizing; Before transplanting, matrix is all through 400 times of carbendazim process, after transplanting, sprays 800 times of carbendazim once weekly, temperature (20 ± 2) DEG C, humidity 85%;
(6) cultivation cycle is one month for the first time, and after group training Establishing, cycle time is two weeks.
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| CN201510214232.1A CN104855286A (en) | 2015-04-22 | 2015-04-22 | Tongling peony tissue culture and rapid propagation seedling raising technical method |
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| CN201510214232.1A CN104855286A (en) | 2015-04-22 | 2015-04-22 | Tongling peony tissue culture and rapid propagation seedling raising technical method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108094197A (en) * | 2017-11-30 | 2018-06-01 | 安徽心缘康生物科技有限公司 | A kind of oil tree peony phoenix pellet asexual multiplication seedling method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108094197A (en) * | 2017-11-30 | 2018-06-01 | 安徽心缘康生物科技有限公司 | A kind of oil tree peony phoenix pellet asexual multiplication seedling method |
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