CN105532477A - Peony rooting culture method - Google Patents
Peony rooting culture method Download PDFInfo
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- CN105532477A CN105532477A CN201610078484.0A CN201610078484A CN105532477A CN 105532477 A CN105532477 A CN 105532477A CN 201610078484 A CN201610078484 A CN 201610078484A CN 105532477 A CN105532477 A CN 105532477A
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- tree peony
- seedling
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- rooting
- perula
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention discloses a peony rooting culture method which comprises the following steps: stripping a few layers of bud scales from peony bulbil, washing with tap water for 30min and soaking and rinsing with carbendazim for 8-10min; sterilizing with 70% alcohol for 20-30s on an ultra-clean workbench; sterilizing again with 2% sodium hypochlorite or 0.1% mercury bichloride for 8min; washing with sterile water for 3-5 times; stripping the rest bud scales; cutting off some tissues of the base, and inoculating the rest part to a solid culture medium to obtain rootless seedlings; and transferring the rootless seedlings to a rooting culture medium to obtain peony seedlings. By adopting the method disclosed by the invention, the rooting rate and elongation of the seedlings can be effectively increased, so that the peony rooting time is shorter, and the survival rate is improved. The rooting time is shortened to 10 days, the maximum root length is 25.58mm, the rooting rate is over 70%, and the root number is 4.8; however, the contrasted rooting time is 20 days, the rooting rate is 10%, the maximum root length is 18.06, and the root number is 2.
Description
Technical field
The present invention is a kind of method being improved tree peony rooting rate and survival rate by hormon.
Background technology
Tree peony belongs to Paeoniaceae Paeonia machaka, as the floriculture history of existing about 1600 so far.The traditional modes of reproduction of tree peony comprises seed propagation and based on the vegetative propagation of grafting, plant division, cuttage, but Traditional breeding processes has significant limitation: seminal propagation can obtain a large amount of nursery stock in a short time, but solid plant species is based on single-lobe, double variety is shaky or seldom solid, seedling variation is large, should not keep the excellent moral character of maternal plant.Tree peony not only occupies critical role in flowers market at home, simultaneously the important outlet flowers of Ye Shi China, and it is the Main way of tree peony Future Development that industrialization is produced, and it is the important technology promoting tree peony industrialization production that application Techniques of in Vitro Culture carries out Fast-propagation.
The group culturation rapid propagating technology of tree peony also exists that the rate of increase is low, difficulty of taking root and the problem such as transplanting survival rate is low, is difficult to be applied to production.Taking root is one of successful committed step of Plant Tissue Breeding, is that Section Moutan trains subject matter urgently to be resolved hurrily.The prescription of rooting medium grasping tree peony, for the difficult problem of taking root solving tree peony, has important theory, actual application value to the improvement of tree peony rooting technique.The minimal medium that Section Moutan training seedling rooting is used in cultivating has MS, improvement MS, wherein that the most frequently used is 1/2MS.The most effective root induction agent is IBA, also has and uses NAA or IAA root induction.Consider the problem such as nutrient, Browning control, there is stronger specific aim, but in view of Varieties of Peony is abundant and medium component is complicated, to different cultivars and explant, be necessary system, synthetically analyze medium component to the impact of rooting of vitro seedling, thus obtain best basic recipe.
Summary of the invention
The technical problem to be solved in the present invention is: tree peony takes root difficulty, and the low survival rate that causes of rooting rate is low, can not production emerge, and the invention provides a kind of tree peony Rapid Rooting reproductive tissue cultural method.
Technical scheme of the present invention is: a kind of tree peony culture of rootage method: peel off tree peony bulbil number layer perula, 30min is rinsed under running water, carbendazim soaks rinsing 8 ~ 10min, then on superclean bench with 70% alcohol sterilizing 20 ~ 30s, use 2% clorox or 0.1% mercuric chloride sterilizing again, sterilization time 8min, aseptic water washing 3 ~ 5 times, peel off residue perula, cut away base segments tissue, be inoculated on solid culture medium by remaining part, the formula of described solid culture medium is: MS+ contains Ca
2+wPM+IBA0.5mg/L+6-BA0.3mg/L+PVP1g/L+ sugar 30g/L+ agar 7g/L, at pH=5.8, temperature 24 ± 1 DEG C, light intensity 2000Lx, Fiber differentiation 30 days under light application time 12h/d, obtain unrooted seedling, proceed on root media by unrooted seedling, the formula of described root media is 1/2MS+IBA1.0mgL
-1+ sucrose 30gL
-1, be 5.8 at PH, temperature 24 ± 1 DEG C, light intensity 2000Lx, Fiber differentiation 30-35 days under light application time 12h/d, obtains tree peony seedling.
A kind of tree peony culture of rootage method: peel off tree peony bulbil number layer perula, 30min is rinsed under running water, carbendazim soaks rinsing 8 ~ 10min, then on superclean bench with 70% alcohol sterilizing 20 ~ 30s, then use 2% clorox or 0.1% mercuric chloride sterilizing, sterilization time 8min, aseptic water washing 3 ~ 5 times, peels off residue perula, cuts away base segments tissue, be inoculated on solid culture medium by remaining part, the formula of described solid culture medium is: MS+ contains Ca
2+wPM+IBA0.5mg/L+6-BA0.3mg/L+PVP1g/L+ sugar 30g/L+ agar 7g/L, at pH=5.8, temperature 24 ± 1 DEG C, light intensity 2000Lx, Fiber differentiation 30 days under light application time 12h/d, obtain unrooted seedling, proceed on root media by unrooted seedling, the formula of described root media is WPM+IBA4mgL
-1+ PVP1.0gL
-1+ sugared 30gL
-1+ Gellangum2gL
-1, be 5.8 at pH, temperature 24 ± 1 DEG C, cultivates under the condition of light intensity 2000Lx, light application time 12h/d, adds 50mg/L ascorbic acid in root media, cultivates 30-35 days, obtains tree peony seedling.
A kind of tree peony culture of rootage method: peel off tree peony bulbil number layer perula, 30min is rinsed under running water, carbendazim soaks rinsing 8 ~ 10min, then on superclean bench with 70% alcohol sterilizing 20 ~ 30s, then use 2% clorox or 0.1% mercuric chloride sterilizing, sterilization time 8min, aseptic water washing 3 ~ 5 times, peels off residue perula, cuts away base segments tissue, be inoculated on solid culture medium by remaining part, the formula of described solid culture medium is: MS+ contains Ca
2+wPM+IBA0.5mg/L+6-BA0.3mg/L+PVP1g/L+ sugar 30g/L+ agar 7g/L, at pH=5.8, temperature 24 ± 1 DEG C, light intensity 2000Lx, Fiber differentiation 30 days under light application time 12h/d, obtain unrooted seedling, unrooted seedling is proceeded on root media, the formula of described root media is WPM+IAA3mg/L+ caffeic acid 1mg/L+PVP1g/L+LH200mg/L+ sugar 30g/L+Gellangum2g/L, be 5.8 at pH, temperature 24 DEG C, light intensity 2000Lx, cultivate 30-35 days under the condition of light application time 12h/d, obtain tree peony seedling.
The invention has the beneficial effects as follows: the present invention can effectively improve seedling rooting rate and elongation, make tree peony rootage duration shorter, improve survival rate.Rootage duration shortens to 10 days, and maximum root is long is 25.58mm, and rooting rate is more than 70%, and radical is 4.8; And the rootage duration of contrast is 20 days, rooting rate is 10%, and maximum root length is 18.06, and radical is 2.
Embodiment
Embodiment 1
A kind of tree peony culture of rootage method: peel off tree peony bulbil number layer perula, 30min is rinsed under running water, carbendazim soaks rinsing 8 ~ 10min, then on superclean bench with 70% alcohol sterilizing 20 ~ 30s, then use 2% clorox or 0.1% mercuric chloride sterilizing, sterilization time 8min, aseptic water washing 3 ~ 5 times, peels off residue perula, cuts away base segments tissue, be inoculated on solid culture medium by remaining part, the formula of described solid culture medium is: MS+ contains Ca
2+wPM+IBA0.5mg/L+6-BA0.3mg/L+PVP1g/L+ sugar 30g/L+ agar 7g/L, at pH=5.8, temperature 24 ± 1 DEG C, light intensity 2000Lx, Fiber differentiation 30 days under light application time 12h/d, obtain unrooted seedling, proceed on root media by unrooted seedling, the formula of described root media is 1/2MS+IBA1.0mgL
-1+ sucrose 30gL
-1, be 5.8 at PH, temperature 24 ± 1 DEG C, light intensity 2000Lx, Fiber differentiation 30-35 days under light application time 12h/d, obtains tree peony seedling, and rooting rate is 72%.
Embodiment 2
A kind of tree peony culture of rootage method: peel off tree peony bulbil number layer perula, 30min is rinsed under running water, carbendazim soaks rinsing 8 ~ 10min, then on superclean bench with 70% alcohol sterilizing 20 ~ 30s, then use 2% clorox or 0.1% mercuric chloride sterilizing, sterilization time 8min, aseptic water washing 3 ~ 5 times, peels off residue perula, cuts away base segments tissue, be inoculated on solid culture medium by remaining part, the formula of described solid culture medium is: MS+ contains Ca
2+wPM+IBA0.5mg/L+6-BA0.3mg/L+PVP1g/L+ sugar 30g/L+ agar 7g/L, at pH=5.8, temperature 24 ± 1 DEG C, light intensity 2000Lx, Fiber differentiation 30 days under light application time 12h/d, obtain unrooted seedling, proceed on root media by unrooted seedling, the formula of described root media is WPM+IBA4mgL
-1+ PVP1.0gL
-1+ sugared 30gL
-1+ Gellangum2gL
-1, be 5.8 at pH, temperature 24 ± 1 DEG C, cultivates under the condition of light intensity 2000Lx, light application time 12h/d, adds 50mg/L ascorbic acid in root media, and cultivate 30-35 days, obtain tree peony seedling, rooting rate is 75%.
Embodiment 3
A kind of tree peony culture of rootage method: peel off tree peony bulbil number layer perula, 30min is rinsed under running water, carbendazim soaks rinsing 8 ~ 10min, then on superclean bench with 70% alcohol sterilizing 20 ~ 30s, then use 2% clorox or 0.1% mercuric chloride sterilizing, sterilization time 8min, aseptic water washing 3 ~ 5 times, peels off residue perula, cuts away base segments tissue, be inoculated on solid culture medium by remaining part, the formula of described solid culture medium is: MS+ contains Ca
2+wPM+IBA0.5mg/L+6-BA0.3mg/L+PVP1g/L+ sugar 30g/L+ agar 7g/L, at pH=5.8, temperature 24 ± 1 DEG C, light intensity 2000Lx, Fiber differentiation 30 days under light application time 12h/d, obtain unrooted seedling, unrooted seedling is proceeded on root media, the formula of described root media is WPM+IAA3mg/L+ caffeic acid 1mg/L+PVP1g/L+LH200mg/L+ sugar 30g/L+Gellangum2g/L, is 5.8 at pH, temperature 24 DEG C, light intensity 2000Lx, cultivate 30-35 days under the condition of light application time 12h/d, obtain tree peony seedling, rooting rate is 83%.
Claims (3)
1. a tree peony culture of rootage method, is characterized in that:
Peel off tree peony bulbil number layer perula, 30min is rinsed under running water, carbendazim soaks rinsing 8 ~ 10min, then on superclean bench with 70% alcohol sterilizing 20 ~ 30s, then use 2% clorox or 0.1% mercuric chloride sterilizing, sterilization time 8min, aseptic water washing 3 ~ 5 times, peels off residue perula, cuts away base segments tissue, be inoculated on solid culture medium by remaining part, the formula of described solid culture medium is: MS+ contains Ca
2+wPM+IBA0.5mg/L+6-BA0.3mg/L+PVP1g/L+ sugar 30g/L+ agar 7g/L, at pH=5.8, temperature 24 ± 1 DEG C, light intensity 2000Lx, Fiber differentiation 30 days under light application time 12h/d, obtain unrooted seedling, proceed on root media by unrooted seedling, the formula of described root media is 1/2MS+IBA1.0mgL
-1+ sucrose 30gL
-1, be 5.8 at PH, temperature 24 ± 1 DEG C, light intensity 2000Lx, Fiber differentiation 30-35 days under light application time 12h/d, obtains tree peony seedling.
2. a tree peony culture of rootage method, is characterized in that:
Peel off tree peony bulbil number layer perula, 30min is rinsed under running water, carbendazim soaks rinsing 8 ~ 10min, then on superclean bench with 70% alcohol sterilizing 20 ~ 30s, then use 2% clorox or 0.1% mercuric chloride sterilizing, sterilization time 8min, aseptic water washing 3 ~ 5 times, peels off residue perula, cuts away base segments tissue, be inoculated on solid culture medium by remaining part, the formula of described solid culture medium is: MS+ contains Ca
2+wPM+IBA0.5mg/L+6-BA0.3mg/L+PVP1g/L+ sugar 30g/L+ agar 7g/L, at pH=5.8, temperature 24 ± 1 DEG C, light intensity 2000Lx, Fiber differentiation 30 days under light application time 12h/d, obtain unrooted seedling, proceed on root media by unrooted seedling, the formula of described root media is WPM+IBA4mgL
-1+ PVP1.0gL
-1+ sugared 30gL
-1+ Gellangum2gL
-1, be 5.8 at pH, temperature 24 ± 1 DEG C, cultivates under the condition of light intensity 2000Lx, light application time 12h/d, adds 50mg/L ascorbic acid in root media, cultivates 30-35 days, obtains tree peony seedling.
3. a tree peony culture of rootage method, is characterized in that:
Peel off tree peony bulbil number layer perula, 30min is rinsed under running water, carbendazim soaks rinsing 8 ~ 10min, then on superclean bench with 70% alcohol sterilizing 20 ~ 30s, then use 2% clorox or 0.1% mercuric chloride sterilizing, sterilization time 8min, aseptic water washing 3 ~ 5 times, peels off residue perula, cuts away base segments tissue, be inoculated on solid culture medium by remaining part, the formula of described solid culture medium is: MS+ contains Ca
2+wPM+IBA0.5mg/L+6-BA0.3mg/L+PVP1g/L+ sugar 30g/L+ agar 7g/L, at pH=5.8, temperature 24 ± 1 DEG C, light intensity 2000Lx, Fiber differentiation 30 days under light application time 12h/d, obtain unrooted seedling, unrooted seedling is proceeded on root media, the formula of described root media is WPM+IAA3mg/L+ caffeic acid 1mg/L+PVP1g/L+LH200mg/L+ sugar 30g/L+Gellangum2g/L, be 5.8 at pH, temperature 24 DEG C, light intensity 2000Lx, cultivate 30-35 days under the condition of light application time 12h/d, obtain tree peony seedling.
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| CN201610078484.0A CN105532477A (en) | 2016-02-04 | 2016-02-04 | Peony rooting culture method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106212289A (en) * | 2016-08-26 | 2016-12-14 | 北京林业大学 | A kind of method setting up oil Paeonia suffruticosa bulbil efficient aseptic culture system |
| CN108094197A (en) * | 2017-11-30 | 2018-06-01 | 安徽心缘康生物科技有限公司 | A kind of oil tree peony phoenix pellet asexual multiplication seedling method |
| CN111418364A (en) * | 2020-05-11 | 2020-07-17 | 广西壮族自治区药用植物园 | Cutting propagation method of vernonia galophylla |
| CN111903522A (en) * | 2020-08-10 | 2020-11-10 | 河南科技大学 | Method for improving rooting rate of peony bulbil |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103270949A (en) * | 2013-05-22 | 2013-09-04 | 河南省农业科学院 | Novel peony tissue culture rooting method |
| CN104920210A (en) * | 2015-05-19 | 2015-09-23 | 北京林业大学 | Simple and efficient rooting method for peony test-tube plantlet |
-
2016
- 2016-02-04 CN CN201610078484.0A patent/CN105532477A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103270949A (en) * | 2013-05-22 | 2013-09-04 | 河南省农业科学院 | Novel peony tissue culture rooting method |
| CN104920210A (en) * | 2015-05-19 | 2015-09-23 | 北京林业大学 | Simple and efficient rooting method for peony test-tube plantlet |
Non-Patent Citations (2)
| Title |
|---|
| 申萍等: "不同封口方式对牡丹试管苗生长的影响", 《河南农业科学》 * |
| 陈静: "酚酸类物质与牡丹试管苗生根关系的研究", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106212289A (en) * | 2016-08-26 | 2016-12-14 | 北京林业大学 | A kind of method setting up oil Paeonia suffruticosa bulbil efficient aseptic culture system |
| CN108094197A (en) * | 2017-11-30 | 2018-06-01 | 安徽心缘康生物科技有限公司 | A kind of oil tree peony phoenix pellet asexual multiplication seedling method |
| CN111418364A (en) * | 2020-05-11 | 2020-07-17 | 广西壮族自治区药用植物园 | Cutting propagation method of vernonia galophylla |
| CN111903522A (en) * | 2020-08-10 | 2020-11-10 | 河南科技大学 | Method for improving rooting rate of peony bulbil |
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Application publication date: 20160504 |
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