CN104849123B - Ex vivo Tumor fixing device and the method that pathological section is made using the device - Google Patents
Ex vivo Tumor fixing device and the method that pathological section is made using the device Download PDFInfo
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- CN104849123B CN104849123B CN201510316275.0A CN201510316275A CN104849123B CN 104849123 B CN104849123 B CN 104849123B CN 201510316275 A CN201510316275 A CN 201510316275A CN 104849123 B CN104849123 B CN 104849123B
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- draw point
- plummer
- knurl
- vitro
- rear wall
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Abstract
The method that pathological section is made the present invention relates to Ex vivo Tumor fixing device and using the device.Device part mainly includes：Side wall positioned at left side and right side；Top surface of the two ends of one plummer respectively with left and right sides side wall is fixedly connected；Two side and the rear surface of plummer are fixedly connected with a rear wall, and a part for the rear wall protrudes from the plummer；Wherein, the upper surface of the plummer is the horizontal bearing face for fixing in vitro knurl, and the preceding surface of side wall is the vertical loading end for fixing in vitro knurl；Cavity is formed between two side and plummer.The present apparatus structure brand-new by designing, realizes the precise positioning of Ex vivo Tumor, so that in vitro knurl when ensure that PET scan with CT scan puts identical, guarantee is provided to make pathological section.
Description
Technical field
It is to be related to a kind of Ex vivo Tumor fixing device and utilize the dress specifically the present invention relates to field of medical equipment
Put the method for making pathological section.
Background technology
, it is necessary to carry out PET and CT scan in vitro knurl in the in vitro knurl experiment of nude mice.
Because of the limitation of current small animal position emission tomography (PET) and CT scan equipment, (PET and CT is to be scanned respectively, then application software
Two groups of scan images are merged), when should ensure that in vitro knurl fixation in scanning process and ensuring PET scan with CT scan
To put should be identical the position of in vitro knurl；Simultaneously again because of the requirement of experiment purpose, PET and CT scan gained image and pathology
Section need to carry out the comparison of layer and layer, and this is accomplished by accurate image registration.
However, existing tumour fixing device can not meet above-mentioned requirements, scanning result largely have impact on
Accuracy.
The content of the invention
The purpose of the present invention is that there is provided a kind of Ex vivo Tumor fixing device to overcome above-mentioned the deficiencies in the prior art.This dress
Put by designing brand-new structure, realize the precise positioning of Ex vivo Tumor, thus when ensure that PET scan and CT scan from
Putting for body knurl is identical, and guarantee is provided to make pathological section.Meanwhile, present invention also offers make pathology using said apparatus
The method of section.
In order to reach above-mentioned purpose, the present invention is adopted the following technical scheme that：
A kind of Ex vivo Tumor fixing device, including：
Side wall positioned at left side and right side；
Top surface of the two ends of one plummer respectively with left and right sides side wall is fixedly connected；
Two side and the rear surface of plummer are fixedly connected with a rear wall, and a part for the rear wall protrudes from described hold
Microscope carrier；
Wherein, the upper surface of the plummer is the horizontal bearing face for fixing in vitro knurl, and the preceding surface of side wall is use
In the vertical loading end of fixed in vitro knurl；
Cavity is formed between two side and plummer.
It is preferred that, the plummer is run through by the draw point of vertical direction, and the end of draw point has cotton thread.
It is preferred that, the rear wall is run through by the draw point of horizontal direction, and the end of draw point has cotton thread.
It is preferred that, level is misaligned through the draw point and the vertical draw point vertical projection through plummer of rear wall.
It is preferred that, level is two through the draw point of rear wall.
It is preferred that, the vertical draw point through plummer is two.
Present invention also offers a kind of method that utilization said apparatus makes pathological section, comprise the following steps：
A, in vitro knurl is placed on the upper surface of plummer, and a part in vitro knurl is leaned against to the preceding surface of rear wall；
B, using afterbody system, the draw point of cotton thread runs through the plummer vertically through cavity, runs through after draw point is passed and vertically
In vitro knurl；
C, the draw point level of cotton thread runs through the rear wall using afterbody system, draw point pass after again level run through in vitro knurl；
D, progress CT scan；
E, the draw point in step B and step C all passed into vitro knurl, a part for cotton thread is stayed in inside in vitro knurl,
The two ends of all cotton threads are respectively placed on the outside of in vitro knurl；
F, progress PET scan, are then merged PET scan image with CT scan image；
G, using the high density shadow of draw point in image obtained by CT scan in step D two faces are determined, then using in step F
The thickness of the fused images of gained makes pathological section.
It is preferred that, the cotton thread in the step B and step C, by the dipped color of dyestuff.
It is preferred that, the dyestuff is Yihong dyestuff.
The beneficial effects of the invention are as follows：
(1) fixation of in vitro knurl is carried out using the upper surface of plummer and the two faces of the preceding surface of rear wall, it is ensured that in vitro
Steadiness of the knurl in scanning process；
(2) in vitro knurl is run through by the cotton thread of horizontal direction and vertical direction, and cotton thread will not leave artifact in scanning process,
Scanning effect is not interfered with；
(3) 2 faces can be determined using the high density shadow of draw point on CT scan image, figure is then merged with CT according to PET
The thickness of picture makes pathological section.
Brief description of the drawings
Fig. 1 is the structural representation of the present apparatus；
Fig. 2 is the state diagram after the present apparatus is run through by draw point；
In figure：1st, preceding surface, 2, upper surface, 3, side wall, 4, cavity, 5, side wall, 6, plummer, 7, rear wall, 8, draw point, 9,
Cotton thread.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in detail.
Embodiment 1：With reference to shown in Fig. 1 and Fig. 2, a kind of Ex vivo Tumor fixing device, including side wall 3 and side wall 5, side wall 3
Be fixedly connected with the top of side wall 5 with same plummer 6, the rear surface of side wall 3, side wall 5 and plummer 6 with one after
Wall 7 is fixedly connected, and a part for the rear wall 7 protrudes from the plummer 6, rear wall 7 is protruded from before the part of plummer 6
Surface 1 can fix in vitro knurl；Wherein, the upper surface 2 of the plummer 6 is the horizontal bearing face for fixing in vitro knurl, side
Cavity 4 is formed between wall 3 and side wall 5 and plummer 6.
The plummer 6 is run through by the draw point 8 of vertical direction, and the end of draw point 8 has cotton thread 9, and the rear wall 7 is by level
The draw point 8 in direction runs through, and the end of draw point 8 also has cotton thread 9.
Meanwhile, level is not weighed through the projection of the draw point 8 and the vertical vertical direction of draw point 8 through plummer 6 of rear wall 7
Close, can preferably realize fixed effect.
Preferably selection is that level is two through the draw point 8 of rear wall 7, and the vertical draw point 8 through plummer 6 is also two
Root.In the Ex vivo Tumor fixing device that the present embodiment is provided, it can also select to be made using 3D printing technique.
The present embodiment additionally provides a kind of method being scanned using above-mentioned Ex vivo Tumor fixing device in vitro knurl, main
The step is wanted to be：
A, in vitro knurl is placed on the upper surface 2 of plummer 6, and a part in vitro knurl is leaned against to the preceding surface of rear wall 7
1；
B, using afterbody system, the draw point 8 of cotton thread 9 runs through the plummer 6 vertically through cavity 4, after draw point 8 is passed and perpendicular
Directly run through in vitro knurl；
C, using afterbody system, the level of draw point 8 of cotton thread 9 runs through the rear wall 7, draw point 8 pass after again level through in vitro
Knurl；
D, progress CT scan；
E, the draw point 8 in step B and step C all passed into vitro knurl, a part for all cotton threads 9 is stayed in vitro knurl
Inside, the two ends of all cotton threads 9 are respectively placed on the outside of in vitro knurl；
F, progress PET scan, are then merged PET scan image with CT scan image；
G, using the high density shadow of draw point 8 in image obtained by CT scan in step D two faces are determined, then utilize step F
The thickness of the fused images of middle gained makes pathological section.
Cotton thread 9 in above-mentioned steps B and step C, is being carried out before use, by the dipped color of dyestuff, dyestuff may be selected to be Yihong
Dyestuff.
The present invention's is substantially：Because PET is used to detect metabolism and the dynamic change of function belongs to functional image, CT is main
Focus on and judge that internal Morphology belongs to structure imaging, thus be difficult using PET tracer in extracorporeal surface or
Inside makes marks, therefore has selected and can have been made marks with the CT images of variable density in antimer.Draw point is inserted in inside tumor
8, two draw points 8 are inserted respectively on two faces contacted with vitro knurl respectively, and draw point 8 is utilized so on CT scan image
High density shadow can determine 2 faces, therefore in pathological section is made, can first determine this 2 faces, then according to PET with
The thickness of CT fused images makes pathological section.
It is worth noting that, when making section, draw point 8 can not be retained inside knurl body, so needing to apply it
He does not disturb the material for making pathological section to replace.Therefore invention applies cotton thread 9, the same of in vitro knurl is being fixed with draw point 8
When, cotton thread 9 is also introduced in passing, in vitro knurl has finally connected 4 cotton threads 9, Yihong dyestuff soaking and dyeing of cotton thread 9, this
Sample, Yihong dyestuff can also make in vitro knurl intrinsic stain when connecting cotton thread 9, and the position of cotton thread 9 and stained tissue can be used as system
Make to determine the foundation of scan position during pathological section.
The foregoing description of the disclosed embodiments, enables professional and technical personnel in the field to realize or using the present invention.
A variety of modifications to embodiment will be apparent for those skilled in the art, as defined herein general
Principle can be realized in other embodiments without departing from the spirit or scope of the present invention, is not described in detail
Part, be prior art, herein without repeating.Therefore, the present invention is not intended to be limited to these implementations shown in this article
Example, and it is to fit to the most wide scope consistent with feature with principles disclosed herein.
Claims (4)
1. a kind of Ex vivo Tumor fixing device, it is characterised in that including：
Side wall positioned at left side and right side；
Top surface of the two ends of one plummer respectively with left and right sides side wall is fixedly connected；
Two side and the rear surface of plummer are fixedly connected with a rear wall, and a part for the rear wall protrudes from the carrying
Platform；
Wherein, the upper surface of the plummer is the horizontal bearing face for fixing in vitro knurl, and the preceding surface of rear wall is for solid
The vertical loading end of fixed in vitro knurl；
Cavity is formed between two side and plummer；
The plummer is run through by the draw point of vertical direction, and the end of draw point has cotton thread；
The rear wall is run through by the draw point of horizontal direction, and the end of draw point has cotton thread；
Level is misaligned through the draw point and the vertical draw point vertical projection through plummer of rear wall.
2. Ex vivo Tumor fixing device according to claim 1, it is characterised in that level is two through the draw point of rear wall
Root.
3. Ex vivo Tumor fixing device according to claim 2, it is characterised in that the vertical draw point through plummer is two
Root.
4. the method that the Ex vivo Tumor fixing device described in a kind of utilization claim 3 makes pathological section, it is characterised in that bag
Include following steps：
A, in vitro knurl is placed on the upper surface of plummer, and a part in vitro knurl is leaned against to the preceding surface of rear wall；
B, the draw point of cotton thread runs through the plummer vertically through cavity using afterbody system, after draw point is passed and vertical through in vitro
Knurl；
C, the draw point level of cotton thread runs through the rear wall using afterbody system, draw point pass after again level run through in vitro knurl；
D, progress CT scan；
E, the draw point in step B and step C all passed into vitro knurl, a part for cotton thread is stayed in inside in vitro knurl, own
The two ends of cotton thread are respectively placed on the outside of in vitro knurl；
F, progress PET scan, are then merged PET scan image with CT scan image；
G, using the high density shadow of draw point in image obtained by CT scan in step D two faces are determined, then utilize gained in step F
Fused images thickness make pathological section；
Cotton thread in the step B and step C, by the dipped color of dyestuff；The dyestuff is Yihong dyestuff.
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| CN201510316275.0A CN104849123B (en) | 2015-06-10 | 2015-06-10 | Ex vivo Tumor fixing device and the method that pathological section is made using the device |
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| CN201510316275.0A CN104849123B (en) | 2015-06-10 | 2015-06-10 | Ex vivo Tumor fixing device and the method that pathological section is made using the device |
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| CN105954104B (en) * | 2016-06-26 | 2023-03-07 | 中国科学院武汉岩土力学研究所 | Rock crack mesoscopic structure detection system and detection method based on PET/CT |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201184852Y (en) * | 2008-04-21 | 2009-01-21 | 山东省肿瘤医院 | Great tissue section material-drawing bench |
| CN201524354U (en) * | 2009-08-21 | 2010-07-14 | 徐子海 | Head neck positioning frame for different-machine PET/CT image fusion |
| CN101987019A (en) * | 2009-08-03 | 2011-03-23 | 徐子海 | Positron emission tomography (PET) and computed tomography (CT) cross-modality medical image fusion method based on wavelet transform |
| CN103310681A (en) * | 2013-07-01 | 2013-09-18 | 中国人民解放军总医院 | In-vitro eyeball fixator |
| CN203234754U (en) * | 2013-04-17 | 2013-10-16 | 中国人民解放军第三军医大学第二附属医院 | Animal fixing bed used for imaging examination |
| CN103691064A (en) * | 2013-12-14 | 2014-04-02 | 中国科学院近代物理研究所 | Device and method for locating dynamic tumor target area in radiotherapy |
| CN104490415A (en) * | 2015-01-12 | 2015-04-08 | 天津医科大学第二医院 | Three-dimensional directional frame for abdominopelvic PET (Position-emission Tomography), CT (Computed Tomography) and MR (Magnetic Resonance) images fusion |
| CN104523287A (en) * | 2015-01-12 | 2015-04-22 | 天津医科大学第二医院 | Stereoscopic orientation frame for PET, CT and MR image fusion of head and neck range |
| CN204683643U (en) * | 2015-06-10 | 2015-10-07 | 孟雪 | Ex vivo Tumor fixture |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4953210B2 (en) * | 2007-10-12 | 2012-06-13 | サクラファインテックジャパン株式会社 | Extracted biological tissue mounting device and method for fixing extracted biological tissue |
-
2015
- 2015-06-10 CN CN201510316275.0A patent/CN104849123B/en not_active Expired - Fee Related
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201184852Y (en) * | 2008-04-21 | 2009-01-21 | 山东省肿瘤医院 | Great tissue section material-drawing bench |
| CN101987019A (en) * | 2009-08-03 | 2011-03-23 | 徐子海 | Positron emission tomography (PET) and computed tomography (CT) cross-modality medical image fusion method based on wavelet transform |
| CN201524354U (en) * | 2009-08-21 | 2010-07-14 | 徐子海 | Head neck positioning frame for different-machine PET/CT image fusion |
| CN203234754U (en) * | 2013-04-17 | 2013-10-16 | 中国人民解放军第三军医大学第二附属医院 | Animal fixing bed used for imaging examination |
| CN103310681A (en) * | 2013-07-01 | 2013-09-18 | 中国人民解放军总医院 | In-vitro eyeball fixator |
| CN103691064A (en) * | 2013-12-14 | 2014-04-02 | 中国科学院近代物理研究所 | Device and method for locating dynamic tumor target area in radiotherapy |
| CN104490415A (en) * | 2015-01-12 | 2015-04-08 | 天津医科大学第二医院 | Three-dimensional directional frame for abdominopelvic PET (Position-emission Tomography), CT (Computed Tomography) and MR (Magnetic Resonance) images fusion |
| CN104523287A (en) * | 2015-01-12 | 2015-04-22 | 天津医科大学第二医院 | Stereoscopic orientation frame for PET, CT and MR image fusion of head and neck range |
| CN204683643U (en) * | 2015-06-10 | 2015-10-07 | 孟雪 | Ex vivo Tumor fixture |
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