CN104849114A - Special slide for comet experiment - Google Patents

Special slide for comet experiment Download PDF

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Publication number
CN104849114A
CN104849114A CN201510243811.9A CN201510243811A CN104849114A CN 104849114 A CN104849114 A CN 104849114A CN 201510243811 A CN201510243811 A CN 201510243811A CN 104849114 A CN104849114 A CN 104849114A
Authority
CN
China
Prior art keywords
comet
special slide
slotted eye
base plate
slide glass
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201510243811.9A
Other languages
Chinese (zh)
Inventor
刘翠艳
顾曼曼
韩春杨
徐婧
何燕飞
康佑汐
周琪
蒋平
唐云
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Anhui Agricultural University AHAU
Original Assignee
Anhui Agricultural University AHAU
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anhui Agricultural University AHAU filed Critical Anhui Agricultural University AHAU
Priority to CN201510243811.9A priority Critical patent/CN104849114A/en
Publication of CN104849114A publication Critical patent/CN104849114A/en
Pending legal-status Critical Current

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  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention discloses a special slide for comet experiment. The special slide comprises a base plate and edge plates, wherein the base plate is a strip flat plate; more than two circular groove holes are formed in the upper surface of the base plate, and the bottom part of each circular groove hole is concave and flat; the vertical edge plates are arranged at the two ends of the base plate; the base plate and the edge plates are of an integral structure which is made of transparent polystyrene plastic. The special slide has the advantages that the structure is simple, and the convenience in use is realized; the material of glass is replaced by the transparent polystyrene, the easiness in reaction with alkaline liquid is avoided, the repeated use is realized, and the resource is saved.

Description

The special slide glass of comet
Technical field
The present invention relates to the special slide glass of a kind of comet.
Background technology
Comet (comet assay) is the common method of Sensitive Detection DNA damage and reparation on individual cell level.
Current conventional comet slide glass has the following disadvantages:
1. at present conventional slide glass is glass material, in experimentation easily and alkaline reaction and bring certain influence to experimental result;
2. glass slide is in the market only 2 holes, 3 holes and 96 holes, slotted eye be by the middle of hydrophobic barrier directly borehole being attached on this microslide be made.Paving is not before glue solidifies to it, and liquid gel easily overflows the circular port surrounded from the space between the water delivery barrier on upper strata and lower floor's microslide, makes that final to spread gel thicknesses inadequate and uneven, while can cause the out-of-flatness of glue face;
3. common microslide is sheet, is inconvenient to pick and place;
4. comet slide glass price is relatively costly in the market, and is single use, and universality is not strong, and waste resource, causes environmental pollution, is not suitable with the development of current conservation-minded society.
Summary of the invention
The technical problem to be solved in the present invention is to provide the special slide glass of a kind of comet.
In order to solve the problems of the technologies described above, the technical solution used in the present invention is: the special slide glass of comet, comprises substrate and side plate, and substrate is bar-shaped plate, and thereon is provided with 2 with the recessed and smooth circular slotted eye of upper bottom portion; The two ends of substrate are provided with vertical side plate; Substrate and side plate are the integrative-structure be made up of transparent polystyrene plastics.
As preferably, circular slotted eye is 4.
As preferably, substrate thickness is 0.9 mm.
As preferably, the radius of circular slotted eye is 10 mm, and the recessed degree of depth of circular slotted eye is 0.6 mm.
As preferably, the height of side plate is 10 mm.
The invention has the beneficial effects as follows:
Structure is simple, and easy to use, material replaces glass by transparent polystyrene, not easily with alkaline reaction, can recycle, economize on resources.
Accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments, the present invention is further detailed explanation.
Fig. 1 is the structural representation of the special slide glass embodiment of comet of the present invention.
In figure, 1-substrate, 2-side plate, the circular slotted eye of 3-.
Embodiment
Fig. 1 is a kind of special 4 hole slide glasses for comet, is made up of substrate 1 and side plate 2.
Substrate 1 is the flat board of strip, is provided with the vertical side plate 2 arranged at the two ends of flat board.
The circular slotted eye 3 digging 4 downward indents in bottom above of substrate, the bottom flat of slotted eye, the radius of circular slotted eye is 10 mm, and the recessed degree of depth is 0.6 mm.Spacing 2 mm of slotted eye.
Dull and stereotyped length is 8.40cm, and width is 3.28cm, and slab-thickness is 0.9 mm, and the thickness bottom slotted eye only has 0.3 mm.The side plate height at two ends is 10 mm.
Substrate and side plate adopt transparent polystyrene to make integrative-structure.
In use, add gel by slotted eye, and make to lay bottom gel smooth, thickness is moderate, can use.
Compared with product on market, the present embodiment has following features:
1. material adopts polystyrene, not easily with alkaline reaction;
2. the circular slotted eye integrally dug out on substrate, make gel be locked in this hole slot, not easily spill and leakage, and bottom flat, and the slotted eye degree of depth is moderate, and gel glue face is laid smooth, thickness is suitable.
3. this plate has 4 hole slots, can carry out the process of 4 samples simultaneously, solves and that cause test findings difference different due to the processing time, and fills up the vacancy of 2 holes on market, 3 holes and 96 hole slide glasses;
4. two ends are provided with vertical side plate, conveniently pick and place;
5. this kind of slide glass is cheap, very easily promotes; And can recycle, economize on resources.
Above-described embodiment of the present invention, does not form limiting the scope of the present invention.Any amendment done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within claims of the present invention.

Claims (5)

1. the special slide glass of comet, is characterized in that: comprise substrate and side plate, described substrate is bar-shaped plate, and described thereon is provided with 2 with the recessed and smooth circular slotted eye of upper bottom portion; The two ends of described substrate are provided with vertical side plate; Described substrate and side plate are the integrative-structure be made up of transparent polystyrene plastics.
2. the special slide glass of comet according to claim 1, is characterized in that: described circular slotted eye is 4.
3. the special slide glass of comet according to claim 1, is characterized in that: described substrate thickness is 0.9 mm.
4. the special slide glass of comet according to claim 1, is characterized in that: the radius of described circular slotted eye is 10 mm, and the recessed degree of depth of circular slotted eye is 0.6 mm.
5. the special slide glass of comet according to claim 1, is characterized in that: the height of described side plate is 10 mm.
CN201510243811.9A 2015-05-13 2015-05-13 Special slide for comet experiment Pending CN104849114A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510243811.9A CN104849114A (en) 2015-05-13 2015-05-13 Special slide for comet experiment

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510243811.9A CN104849114A (en) 2015-05-13 2015-05-13 Special slide for comet experiment

Publications (1)

Publication Number Publication Date
CN104849114A true CN104849114A (en) 2015-08-19

Family

ID=53848927

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201510243811.9A Pending CN104849114A (en) 2015-05-13 2015-05-13 Special slide for comet experiment

Country Status (1)

Country Link
CN (1) CN104849114A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017214822A1 (en) * 2016-06-13 2017-12-21 石庆学 Sample adding plate for single cell gel electrophoresis

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2793560A1 (en) * 1999-09-07 2000-11-17 Commissariat Energie Atomique Analytical support, useful e.g. for immunological or hybridization assays, comprises analytical sites, each associated with a resonant microcavity for emitting light
US6174683B1 (en) * 1999-04-26 2001-01-16 Biocept, Inc. Method of making biochips and the biochips resulting therefrom
CN1335501A (en) * 2001-04-27 2002-02-13 上海晶泰生物技术有限公司 Multiple-sample microarray biochip
CN1343887A (en) * 2001-08-07 2002-04-10 东南大学 Process for preparing antigen microarray based on self antibody repertoire
CN1435488A (en) * 2002-02-01 2003-08-13 先进基因股份有限公司 Plastic substrate useful for making bio-chip
CN1519329A (en) * 2003-01-24 2004-08-11 财团法人工业技术研究院 Reactor of biochip in micro array
CN1547668A (en) * 2002-03-15 2004-11-17 索尼公司 Substrate for bioassay, bioassay apparatus, and reading apparatus
CN1771438A (en) * 2003-04-10 2006-05-10 库诺公司 Improved composite microarry slides
CN1818648A (en) * 2006-03-15 2006-08-16 北京博奥生物芯片有限责任公司 Agarose gel plastic substrate, its production and use
CN102297858A (en) * 2010-06-24 2011-12-28 中国科学院生态环境研究中心 Test tube method and apparatus for testing SOS / umu genotoxic effect
CN102719352A (en) * 2012-06-06 2012-10-10 西安交通大学 Cell chip slide for preparing microarray cell chips and preparation method

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6174683B1 (en) * 1999-04-26 2001-01-16 Biocept, Inc. Method of making biochips and the biochips resulting therefrom
FR2793560A1 (en) * 1999-09-07 2000-11-17 Commissariat Energie Atomique Analytical support, useful e.g. for immunological or hybridization assays, comprises analytical sites, each associated with a resonant microcavity for emitting light
CN1335501A (en) * 2001-04-27 2002-02-13 上海晶泰生物技术有限公司 Multiple-sample microarray biochip
CN1343887A (en) * 2001-08-07 2002-04-10 东南大学 Process for preparing antigen microarray based on self antibody repertoire
CN1435488A (en) * 2002-02-01 2003-08-13 先进基因股份有限公司 Plastic substrate useful for making bio-chip
CN1547668A (en) * 2002-03-15 2004-11-17 索尼公司 Substrate for bioassay, bioassay apparatus, and reading apparatus
CN1519329A (en) * 2003-01-24 2004-08-11 财团法人工业技术研究院 Reactor of biochip in micro array
CN1771438A (en) * 2003-04-10 2006-05-10 库诺公司 Improved composite microarry slides
CN1818648A (en) * 2006-03-15 2006-08-16 北京博奥生物芯片有限责任公司 Agarose gel plastic substrate, its production and use
CN102297858A (en) * 2010-06-24 2011-12-28 中国科学院生态环境研究中心 Test tube method and apparatus for testing SOS / umu genotoxic effect
CN102719352A (en) * 2012-06-06 2012-10-10 西安交通大学 Cell chip slide for preparing microarray cell chips and preparation method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017214822A1 (en) * 2016-06-13 2017-12-21 石庆学 Sample adding plate for single cell gel electrophoresis

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Application publication date: 20150819