CN104841359A - Method for processing silica membrane - Google Patents
Method for processing silica membrane Download PDFInfo
- Publication number
- CN104841359A CN104841359A CN201410050181.9A CN201410050181A CN104841359A CN 104841359 A CN104841359 A CN 104841359A CN 201410050181 A CN201410050181 A CN 201410050181A CN 104841359 A CN104841359 A CN 104841359A
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- CN
- China
- Prior art keywords
- silica membrane
- pellosil
- nucleic acid
- completely
- treatment fluid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
The invention relates to a method for processing a silica membrane. The method is characterized by: contacting the silica membrane with a treating fluid; soaking the silica membrane with the treating fluid; taking the silica membrane out till the silica membrane is completely soaked; and putting the silica membrane in an oven at a temperature range of 50-70 DEG C to enable water on the silica membrane to be totally dried off. The treating liquid is an inorganic solution of Na2CO3 or NaHCO3 and has a saturation concentration. The invention has advantages that the silica membrane is treated through the method; the treated silica membrane is high in DNA specific absorption; the extraction yield and recycling yield of nucleic acid are improved in a larger degree; the silica membrane has excellent uniformity and stability; the bioavailability is improved; and the function of extracting nucleic acid is effectively developed. The method is simple in technology and easy to operate.
Description
Technical field
The present invention is specifically related to a kind of processing method of pellosil.
Background technology
Since having silica gel, silica gel has a wide range of applications in the middle of all trades and professions and daily life.Silica gel (Silica gel; Silica) call: silica gel is a kind of high activity sorbing material, belong to amorphous substance, its chemical molecular formula is mSiO2nH2O.Water insoluble and any solvent, nonpoisonous and tasteless, stable chemical nature, does not react with any material except highly basic, hydrofluoric acid.The silica gel of various model forms different microcellular structures because its manufacture method is different.The chemical composition of silica gel and physical arrangement, determine it and have other same type of material many and be difficult to replace to obtain feature: absorption property is high, Heat stability is good, stable chemical nature, have higher mechanical strength etc.
Pellosil adopts film technique to suppress by silica gel particle and paper pulp and forms, can be used for DNA, the absorption of RNA, is the suggesting material making DNA purification column.But directly use pellosil method be used for separate nucleic acid exist pellosil instability, heterogeneity and with degradation shortcoming under nucleic acid binding ability.
Summary of the invention
The object of the invention is the shortcoming in order to overcome prior art, providing a kind of pellosil processing method improving pellosil DNA absorption property, reduce impurity in products, improve extraction efficiency.The processing method of a kind of pellosil of the present invention, is characterized in that: contacted with treatment fluid by pellosil, makes treatment fluid soak into pellosil completely; After pellosil soaks completely, take out pellosil, pellosil is placed in 50 ~ 70 DEG C of baking ovens, dry the moisture on pellosil completely.
Described treatment fluid is Na2CO3 or NaHCO3 inorganic solution, and concentration is saturated concentration.
The invention has the advantages that: adopt this method process pellosil, pellosil after treatment adsorbs DNA high specific, nucleic acid extraction output and recovery yield are all greatly increased, and make pellosil have good homogeneity and stability, improve its bioavilability, its nucleic acid extraction function of more effective performance.And technique is simple, be easy to operation.
Detailed description of the invention
A processing method for pellosil, is characterized in that: contacted with treatment fluid by pellosil, makes treatment fluid soak into pellosil completely; After pellosil soaks completely, take out pellosil, pellosil is placed in 50 ~ 70 DEG C of baking ovens, dry the moisture on pellosil completely.
Described treatment fluid is Na2CO3 or NaHCO3 inorganic solution, and concentration is saturated concentration.
Above-described embodiment is only in order to illustrate technical scheme of the present invention but not to limit design of the present invention and protection domain; those of ordinary skill of the present invention is modified to technical scheme of the present invention or equivalent replacement; and not departing from aim and the scope of technical scheme, it all should be encompassed in right of the present invention.
Claims (3)
1. a processing method for pellosil, is characterized in that: contacted with treatment fluid by pellosil, makes treatment fluid soak into pellosil completely.
2. the processing method of a kind of pellosil according to claim 1, is characterized in that: after pellosil soaks completely, take out pellosil, pellosil is placed in 50 ~ 70 DEG C of baking ovens, dries the moisture on pellosil completely.
3. the processing method of a kind of pellosil according to claim 2, is characterized in that: described treatment fluid is Na2CO3 or NaHCO3 inorganic solution, and concentration is saturated concentration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410050181.9A CN104841359A (en) | 2014-02-14 | 2014-02-14 | Method for processing silica membrane |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410050181.9A CN104841359A (en) | 2014-02-14 | 2014-02-14 | Method for processing silica membrane |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104841359A true CN104841359A (en) | 2015-08-19 |
Family
ID=53841572
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410050181.9A Pending CN104841359A (en) | 2014-02-14 | 2014-02-14 | Method for processing silica membrane |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104841359A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107653242A (en) * | 2017-10-30 | 2018-02-02 | 上海捷瑞生物工程有限公司 | A kind of pellosil treatment fluid and preparation method thereof |
-
2014
- 2014-02-14 CN CN201410050181.9A patent/CN104841359A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107653242A (en) * | 2017-10-30 | 2018-02-02 | 上海捷瑞生物工程有限公司 | A kind of pellosil treatment fluid and preparation method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150819 |
|
| WD01 | Invention patent application deemed withdrawn after publication |