CN104833812A - Portable full-automatic biochemical analysis device - Google Patents

Portable full-automatic biochemical analysis device Download PDF

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Publication number
CN104833812A
CN104833812A CN201510240355.2A CN201510240355A CN104833812A CN 104833812 A CN104833812 A CN 104833812A CN 201510240355 A CN201510240355 A CN 201510240355A CN 104833812 A CN104833812 A CN 104833812A
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CN
China
Prior art keywords
chip
locking
ratchet
rotary
tray
Prior art date
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Granted
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CN201510240355.2A
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Chinese (zh)
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CN104833812B (en
Inventor
王战会
裴臣超
朱焱
江伟
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Micro Nano Core Suzhou Technology Co ltd
Tianjin Weinanxin Technology Co ltd
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TIANJIN MICRO NANO CHIP TECHNOLOGY Co Ltd
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Application filed by TIANJIN MICRO NANO CHIP TECHNOLOGY Co Ltd filed Critical TIANJIN MICRO NANO CHIP TECHNOLOGY Co Ltd
Priority to CN201510240355.2A priority Critical patent/CN104833812B/en
Publication of CN104833812A publication Critical patent/CN104833812A/en
Priority to PCT/CN2016/076987 priority patent/WO2016180087A1/en
Application granted granted Critical
Publication of CN104833812B publication Critical patent/CN104833812B/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor

Abstract

The invention relates to a portable full-automatic biochemical analysis device which comprises a shell, a power supply, a control module and a core module, wherein the core module comprises a chip provided with at least one detection trough, a chip holding part capable of entering or exiting the shell, a locking device arranged on the chip holding part and used for fixing and locking a chip, a rotary driving part used for enabling the chip to rotate, a light source part of irradiating light in the chip detection trough, and a detection part used for receiving light penetrating through the detection trough, wherein the rotary driving part, the light source part and the detection part are respectively in communication with the control module. The portable full-automatic biochemical analysis device disclosed by the invention can be used for detecting a micro-fluidic chip, is small in volume, is portable, and is suitable for in-site instant detection; and meanwhile, the chip can realize automation from separation to detection, and is high in detection efficiency.

Description

Portable type full-automatic biochemical analyser
Technical field
The present invention relates to biochemistry detection field, particularly a kind of portable biochemical pick-up unit detected for micro-fluidic chip.
Background technology
Early 1990s, Manz and Widmer etc. propose the concept of Microfluid based Lab on a chip (lab-on-a-chip), also referred to as micro-total analysis system (Miniaturized TotalAnalysis Systems, μ-TAS).Chemistry or biology laboratory can be integrated on the chip of several square centimeters by micro-fluidic chip, carry out sample pretreatment, separation and detection etc., by to the Highgrade integration of chemical device with microminiaturized, to greatest extent the function of assay laboratory is transferred in portable equipment.Present stage, it has been widely used in the various fields such as biology, pharmacodiagnosis, genetic analysis, environmental monitoring; Simultaneously, because it is small-sized, portable, advantage easily, micro-fluidic chip also shows remarkable advantage at clinical medicine domain, makes micro-fluidic chip can realize " scene is detected immediately " (Point of care, POC), significant to global public health.
The fast-developing of micro-fluidic chip brings new opportunity on the one hand on-the-spot instant detection, also has higher requirement to pick-up unit on the other hand.At present, pick-up unit based on micro-fluidic chip mainly comprises optical detection (as fluorescence, absorbance, chemiluminescence, plasma resonance etc.), Electrochemical Detection (as ampere, current potential, conductance etc.) and Mass Spectrometer Method etc., and the usual volume of these devices is larger, be mainly used in experimental study, should not carry, the demand that tools for doctor home visit carries can not be met, the realization that the structure how optimizing portable detector makes it real is carried with, become tools for doctor home visit medical treatment bag in a member have great importance.
At present, prior art JP-JP-2006-110491 discloses a kind of microchip whirligig, by carrying out centrifuging to importing the microchip applying centrifugal force treating corpse or other object for laboratory examination and chemical testing blood, blood plasma after centrifugal and reagent are mixed as detected object liquid, then imports to the microchip centrifugal separating device of absorption photometric measurement section.But it needs to be taken out by chip after centrifugation, then carries out photometric analysis of extinction, cannot realize integration, detect in time, affect detection efficiency.Prior art CN200710126374.8 discloses a kind of modular microchip inspection apparatus, can be used for blood analysis, and can centrifugal and detecting step be integrated in a chip, but this testing fixture chip mounting portion is only applicable to the single channel chip detection of given shape, and chip is arranged around armature spindle both sides, pick-up unit volume is increased, and is not suitable for hyperchannel and detects fast.Prior art CN201120521940.7 disclose a kind ofly can to fix, the trivial tight and electric machine assembly that drives circular biochip to rotate, it can realize stopping the trivial of circular chip, this electric machine assembly can be used in microchip detector, but its Suo Jin mechanism comprises the external auxiliary structure of lock shaft, lock cam structure and complexity, complex structure, volume are large, cause whole testing fixture portability poor.
Summary of the invention
The present invention, for overcoming the shortcomings such as prior art flexible biological probe portability is poor, complex structure, detection efficiency are low, provides a kind of Portable type full-automatic biochemical analyser.
In order to realize object of the present invention, the present invention adopts following technical scheme:
A kind of Portable type full-automatic biochemical analyser, is characterized in that: comprise housing, power supply, control module and nucleus module; Described nucleus module comprises: be provided with the chip that at least one detects groove, the chip maintaining part of housing can be passed in and out, be arranged on the locking device for fixing and lock chip in chip maintaining part, the rotary driving part rotated for making chip, light is radiated at the light source portion in chip detection groove, for accepting the probe portion through detecting groove light; Wherein, described rotary driving part, light source portion and probe portion are connected with control module communication respectively.
Preferably, described control module is arranged on above enclosure interior; Power supply and nucleus module are arranged on below enclosure interior.Preferably, described control module can be master control board card, and described power supply can be battery.
Preferably, described chip maintaining part comprises turnover storehouse frame and transmission gear; Described turnover storehouse frame arranges a through hole, for laying chip locking device.
Preferably, described transmission gear is positioned at inside the frame of turnover storehouse, carries out transmission by internal tooth drive mode.
Preferably, described rotary driving part is motor, and described driven by motor motor shaft rotates and then drives chip to rotate.Preferred, described motor is DC brushless motor.
Preferably, described chip lower surface central authorities comprise a circular groove, and described circular groove is circumferentially provided with at least one locating piece and at least one locking hole.Preferred, described chip is circular, and described detection groove is multiple and evenly arranges along chip circumference.
Preferably, described locking device comprises rotary-tray and is fixed on the locking disk above rotary-tray, and described rotary-tray is coaxial with locking disk, and described rotary-tray diameter is greater than described locking disk; Wherein, described locking disk circumference is provided with at least one detent and at least one locking member; Described detent is arranged on outside described locking disk, with the locating piece of chip with the use of, there is circumference movement for preventing between chip and locking disk; Described locking member comprises the stay of band spring, and described spring is arranged in the lock chambers of locking disk inside, the extended lock chambers of described stay, with the locking hole of chip with the use of, move axially for preventing chip.Wherein stay can be regular shape or irregular sheet or hook-shaped, and described stay can have certain thickness, can show as spherical or block shape convex shape in some cases.
Preferably, described rotary-tray is provided with slipmat.
Preferably, the quantity of described detent and locking member is respectively 2-10, preferred, and the quantity of described detent and locking member is 3-6, and such as 3,4,5,6.
Preferably, described detent and locking member are evenly arranged along locking disk circumference, and spaced.
Preferably, described locking device also comprises locking Connection Block, and described locking Connection Block is positioned at below rotary-tray.Described locking Connection Block and rotary-tray can be structure as a whole, and also can be separate parts, when both are separate parts, by securing member as lockings such as bolts.
Preferably, described locking Connection Block is fixed on described rotary driving part by jackscrew.Described rotary driving part drives locking Connection Block to rotate driven rotary pallet then and rotates.
Preferably, described locking device also comprises ratchet stop rotation mechanism, and described ratchet stop rotation mechanism comprises ratchet, V-arrangement ratchet, spline spring and the register pin of being with ratchet slot; Wherein, described ratchet is arranged on below locking Connection Block, described spline spring and register pin are separately positioned in chip maintaining part, the pawl of described V-arrangement ratchet is first can be stretched in ratchet slot under the elastic force effect of spline spring, the pawl head two of described V-arrangement ratchet is connected with register pin, and register pin can be with that pawl is first leaves ratchet slot.
Beneficial effect of the present invention:
1. little, the good portability of volume.The each modular construction of biochemical analyser of the present invention is compact, is reduced the volume of device by each parts rational deployment greatly, can really realize on-the-spot instant analysis.Such as, adopt internal tooth drive mode, reduce the volume of turnover storehouse frame Width; The ingehious design of locking mechanism and chip, also saves space, and device volume is reduced further.
2. automatic analysis, high flux detect.Chip can realize robotization from being separated to detection, only needs component to be checked to add in chip, can carry out fast being separated, each Indexs measure in analytical equipment, substantially increases detection efficiency.
Accompanying drawing explanation
Fig. 1 biochemical analyser structural representation of the present invention
The each component layouts figure of Fig. 2 biochemical analyser of the present invention
Fig. 3 biochemical analyser chip of the present invention holding part is in outside schematic diagram
The each part position relations schematic diagram of Fig. 4 biochemical analyser of the present invention
Fig. 5 chip structure schematic diagram of the present invention
Fig. 6 chip maintaining part of the present invention structural representation
Fig. 7 locking device structure schematic diagram of the present invention
Fig. 8 locking device diagrammatic cross-section of the present invention
The locking device structure schematic diagram of Fig. 9 translator of the present invention
Locking device structure schematic diagram after Figure 10 chip of the present invention
Figure 11 ratchet stop rotation mechanism of the present invention structural representation
Embodiment
Below in conjunction with accompanying drawing, embodiment of the present invention is clearly and completely described.
See accompanying drawing 1-2, Portable type full-automatic biochemical analyser 1 of the present invention, comprises housing 10, power supply 20, control module 30 and nucleus module 40; Wherein, nucleus module 40 comprises: be provided with the chip 100 that at least one detects groove, the chip maintaining part 200 of housing can be passed in and out, be arranged on the locking device 300 for fixing and lock chip in chip maintaining part, the rotary driving part 400 rotated for making chip, light is radiated at the light source portion 500 in chip detection groove, for accepting the probe portion 600 through detecting groove light; Wherein, rotary driving part 400, light source portion 500 and probe portion 600 are connected with control module 30 communication respectively.
When detecting, liquid to be checked is added in chip 100, then chip maintaining part 200 is made to eject housing 10 inner, see accompanying drawing 3, chip 100 is put into chip maintaining part 200, chip is locked by locking device 300, chip maintaining part 200 is made to enter enclosure interior again, control rotary driving part by control module to rotate, thus drive chip to rotate, liquid to be checked in chip is separated under the influence of centrifugal force, and enter in detection groove, control rotary driving part stops operating, control the detection light that light source portion sends certain wavelength, received by probe portion after detecting the liquid to be checked in light transmission detection groove, and send data to control module.
In a concrete embodiment, see accompanying drawing 3, housing 10 arranged outside has display screen 50.
In a concrete embodiment, power supply 20 can be battery, is arranged on enclosure interior, can meet portable demand of paying a home visit.Control module 30 can be master control board card, for control rotary driving part drive chip to rotate or stop, controlling light source portion launch in chip detection groove certain wavelength ripple, collect the detection data that probe portion sends, and carry out analyzing and processing.
In a concrete embodiment, in order to reduce the volume of analytical equipment as far as possible, making it more portable, being optimized the location layout of each parts of analytical equipment, see accompanying drawing 4, control module 30 is arranged on housing 10 inner upper; Power supply 20 and nucleus module 40 are arranged on below enclosure interior.Wire harness between connection nucleus module 40 and control module 30, by longitudinal layout, reduces the size on analytical equipment Width.Concrete, control module is connected by connector 60 and wire harness 70 communication with nucleus module.
See accompanying drawing 5, in the embodiment of the invention, chip 100 can be circular chip, and chip 100 lower surface central authorities comprise a circular groove 110, and the shape of groove 110 is mated with locking device 300; The axis of circular groove 110 is provided with at least one locating piece 120 and locking hole.In a concrete embodiment, in order to improve detection efficiency, realize multi objective, high flux that is multi-functional, Multi-example detects, chip 100 circumference is evenly provided with multiple detection groove 140, and the quantity detecting groove can be chosen according to the actual requirements.More specifically, this circular chip can be the multilayer chiop comprising lead channel, reservoir, multiple detection groove, the such as chip structure shown in prior art CN201110416978.2, CN201110086070.X, CN201210046040.0.
See accompanying drawing 6, in the embodiment of the invention, chip maintaining part 200 comprises turnover storehouse frame 210 and transmission gear 220; Wherein, pass in and out on storehouse frame 210 and a through hole 211 is set, for laying chip locking device 300.The present invention can free in and out the turnover chamber structure of enclosure interior by arranging, under the state of delivering from godown, be convenient to install and change chip, after warehouse entry, can ensure that chip is in dark surrounds when detecting, avoiding external stray light Interference Detection.Further, transmission gear 220, in order to reduce the size of chip maintaining part, is arranged on inside turnover storehouse frame 210 by the present invention, and is positioned at gear pass in and out outside the frame of storehouse and carries out internal tooth drive, the warehouse entry realizing chip with deliver from godown.
See accompanying drawing 7-8, in the embodiment of the invention, for locking the locking device 300 of chip, the locking disk 320 comprising rotary-tray 310 and be fixed on above rotary-tray 310, rotary-tray 310 is coaxial with locking disk 320, and rotary-tray 310 diameter is greater than locking disk 320.In a concrete embodiment, rotary-tray 310 and locking disk 320 can be structure as a whole, also by individual components assembling (being structure as a whole shown in accompanying drawing 5).
Wherein, rotary-tray 310 is for laying chip 100, see accompanying drawing 5, chip can be circular chip, chip 100 lower surface central authorities comprise a circular groove 110, and the shape of groove 110 is mated, when laying chip with locking disk 320, the groove 110 of chip 100 is aimed at locking disk 320, presses downwards.Contact with rotary-tray outside chip 100 lower surface annulus.
See accompanying drawing 7-8, locking disk 320 circumference is provided with at least one detent 321 and at least one locking member 322 (accompanying drawing is depicted as 3).In a concrete embodiment, the quantity of detent 321 and locking member 322 is 2-10, and its quantity can be chosen according to the actual requirements, and what the quantity of detent 321 and locking member 322 was concrete can be 3-6, such as 3,4,5,6.
In order to ensure that coupling mechanism force is even, detent 321 and locking member 322 are evenly arranged along locking disk 320 circumference, and spaced layout.
See accompanying drawing 5,7-8, detent 321 is arranged on outside locking disk 320, with the locating piece 120 of chip 100 with the use of, there is circumference movement for preventing between chip 100 and locking disk 320.The shape of detent 321 and chip positioning block 120 match in actual applications, and locating piece 120 may be stuck in detent 321, and limited chip 100 is moved in XY face.
See accompanying drawing 5,7-8, locking member 322 comprises the stay 324 of band spring 323, wherein, spring 322 is arranged in the lock chambers 325 of locking disk 320 inside, stay 325 is extended lock chambers 325 under the effect of spring 323 elastic force, with the locking hole of chip 100 with the use of, move axially for preventing chip.When laying chip, press chip downwards, stay 323 is retracted in lock chambers 325, continue to press downwards, stay 323 ejects and enters in the locking hole of chip 100 under elastic force effect, by the crossed strip power locking chip of spring, prevents chip from ejecting when high-speed rotation.Wherein stay can be regular shape or irregular sheet or hook-shaped, and described stay can have certain thickness, can show as spherical or block shape convex shape in some cases.
In the embodiment that the present invention one is concrete, see accompanying drawing 8, in order to prevent mutually sliding between chip 100 and rotary-tray 310, rotary-tray upper surface is provided with slipmat 311, when chip 100 is placed in rotary-tray 310, between slipmat and chip, friction force increases, and further increases locking effect.
In the embodiment that the present invention one is concrete, rotary driving part 400 of the present invention can be motor 410, and motor 410 rotates for driven rotary pallet 310, then drives chip 100 fixed thereon to rotate.Motor can be DC brushless motor, also can be other conventional motor of this area.
In the embodiment that the present invention one is concrete, see accompanying drawing 7-8, locking device 300 of the present invention also can comprise locking Connection Block 330, and locking Connection Block 330 is positioned at below rotary-tray 310, and coaxially arranges.Locking Connection Block 330 can be structure as a whole with rotary-tray 310, also can be separate parts, when both are separate parts, by securing member as bolt etc. is locked (accompanying drawing is depicted as separate parts).
In the embodiment that the present invention one is concrete, see accompanying drawing 9-10, locking Connection Block 330 is fixed on the motor shaft 420 of motor 410 by jackscrew 331.When starter motor, driven by motor locking Connection Block 330 rotates driven rotary pallet 310 then and rotates, and then drives chip to rotate.
In the embodiment that the present invention one is concrete, see accompanying drawing 11, in order to avoid in the process of laying chip, rotary-tray rotates, and cause locking difficulty to increase, locking device 300 of the present invention can arrange ratchet stop rotation mechanism 340.Ratchet stop rotation mechanism 340 is arranged on below locking Connection Block 330, comprises the ratchet 342 of band ratchet slot 341, V-arrangement ratchet 343, spline spring 344 and register pin 345.Wherein, the pawl first 346 of V-arrangement ratchet 343 can stretch in ratchet slot 341 under the elastic force effect of spline spring 344, the pawl head 2 347 of V-arrangement ratchet 343 is connected with register pin 345, and register pin 345 can press downwards pawl head 2 347, is then with pawl first 346 to leave ratchet slot 341.When pawl is first stretch into ratchet slot time, ratchet slot spline, makes locking Connection Block stop operating, then makes rotary-tray spline; When pawl is first leave ratchet slot time, can starter motor driven rotary pallet rotate.In an embodiment, spline spring 344 and register pin 345 can be fixed in the turnover storehouse frame 210 of testing fixture, when be in deliver from godown state time, spline spring 344 ejects, under elastic force effect by first for pawl 346 press-in ratchet slots 341 in; When after warehouse entry, register pin 345 acts on, and presses pawl head 2 347 downwards, is then with pawl first 346 to leave ratchet slot 341.
Above-described embodiment is only for illustrating technical conceive of the present invention and feature, and not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement etc., all should be included within protection scope of the present invention.

Claims (12)

1. a Portable type full-automatic biochemical analyser, is characterized in that: comprise housing, power supply, control module and nucleus module;
Described nucleus module comprises: be provided with the chip that at least one detects groove, the chip maintaining part of housing can be passed in and out, be arranged on the locking device for fixing and lock chip in chip maintaining part, the rotary driving part rotated for making chip, light is radiated at the light source portion in chip detection groove, for accepting the probe portion through detecting groove light; Wherein, described rotary driving part, light source portion and probe portion are connected with control module communication respectively.
2. analytical equipment as claimed in claim 1, is characterized in that: described control module is arranged on above enclosure interior; Described power supply and nucleus module are arranged on below enclosure interior.
3. analytical equipment as claimed in claim 1, is characterized in that: described chip maintaining part comprises turnover storehouse frame and transmission gear; Described turnover storehouse frame arranges a through hole, for laying chip locking device.
4. analytical equipment as claimed in claim 3, is characterized in that: described transmission gear is positioned at inside the frame of turnover storehouse, carries out transmission by internal tooth drive mode.
5. analytical equipment as claimed in claim 1, is characterized in that: described rotary driving part is motor, and described driven by motor motor shaft rotates and then drives chip to rotate.
6. the analytical equipment as described in any one of claim 1-5, is characterized in that: described chip lower surface central authorities comprise a circular groove, and described circular groove is circumferentially provided with at least one locating piece and at least one locking hole.
7. analytical equipment as claimed in claim 6, is characterized in that: described locking device comprises rotary-tray and is fixed on the locking disk above rotary-tray, and described rotary-tray is coaxial with locking disk, and described rotary-tray diameter is greater than described locking disk; Wherein, described locking disk circumference is provided with at least one detent and at least one locking member; Described detent is arranged on outside described locking disk, with the locating piece of chip with the use of, there is circumference movement for preventing between chip and locking disk; Described locking member comprises the stay of band spring, and described spring is arranged in the lock chambers of locking disk inside, the extended lock chambers of described stay, with the locking hole of chip with the use of, move axially for preventing chip.
8. analytical equipment as claimed in claim 7, is characterized in that: the quantity of described detent and locking member is respectively 2-10, and described detent and locking member are evenly arranged along locking disk circumference, and spaced.
9. analytical equipment as claimed in claim 7, is characterized in that: described rotary-tray is provided with slipmat.
10. the analytical equipment as described in any one of claim 7-9, is characterized in that: described locking device also comprises locking Connection Block, and described locking Connection Block is positioned at below rotary-tray.
11. analytical equipments as claimed in claim 10, is characterized in that: described locking Connection Block is fixed on described rotary driving part by jackscrew.
12. analytical equipments as claimed in claim 10, is characterized in that: described locking device also comprises ratchet stop rotation mechanism, and described ratchet stop rotation mechanism comprises ratchet, V-arrangement ratchet, spline spring and the register pin of being with ratchet slot; Wherein, described ratchet is arranged on below locking Connection Block, described spline spring and register pin are separately positioned in chip maintaining part, the pawl of described V-arrangement ratchet is first can be stretched in ratchet slot under the elastic force effect of spline spring, the pawl head two of described V-arrangement ratchet is connected with register pin, and register pin can be with that pawl is first leaves ratchet slot.
CN201510240355.2A 2015-05-12 2015-05-12 Portable type full-automatic biochemical analyser Active CN104833812B (en)

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PCT/CN2016/076987 WO2016180087A1 (en) 2015-05-12 2016-03-22 Portable full-automatic biochemical analysis device

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WO2016180087A1 (en) * 2015-05-12 2016-11-17 天津微纳芯科技有限公司 Portable full-automatic biochemical analysis device
CN111579766A (en) * 2020-05-25 2020-08-25 成都微康生物科技有限公司 Disc-type microfluidic fluorescence immunoassay analyzer and immunoassay item testing method
CN112557682A (en) * 2020-12-14 2021-03-26 广州万孚生物技术股份有限公司 Micro-fluidic biochemical analyzer

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