CN104830727A - Methylobacterium capable of degrading chlorohydrocarbon and applications thereof - Google Patents

Methylobacterium capable of degrading chlorohydrocarbon and applications thereof Download PDF

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CN104830727A
CN104830727A CN201510225653.4A CN201510225653A CN104830727A CN 104830727 A CN104830727 A CN 104830727A CN 201510225653 A CN201510225653 A CN 201510225653A CN 104830727 A CN104830727 A CN 104830727A
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methylobacterium
chlorohydrocarbon
hydrochloric ether
chloro
degradable
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CN104830727B (en
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张丽杰
何芝
赵天涛
全学军
邢志林
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Chongqing University of Technology
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
    • A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C1/00—Reclamation of contaminated soil
    • B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/36—Organic compounds containing halogen

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Abstract

The invention relates to a methylobacterium capable of degrading chlorohydrocarbon and applications thereof. The preservation number of methylobacterium strain is CCTCC No. M2015046. The methylobacterium has a high performance on tolerating chlorohydrocarbon, can utilize chlorohydrocarbon as the only carbon source and energy to grow, overcomes the shortages of co-metabolism degradation technology, can maintain a high activity under a nutrient-insufficient environment, can be used in the fields such as wastewater processing, drinking water recovery, soil recovery, and the like, and is advantageous to achieve a break in the field of chlorohydrocarbon biological degradation.

Description

The Methylobacterium of degradable hydrochloric ether and application thereof
Technical field
The invention belongs to technical field of environmental microorganism, be specifically related to a kind of Methylobacterium and application thereof of degradable hydrochloric ether.
Background technology
Hydrochloric ether, as important organic solvent and product intermediate, is widely used in the fields such as chemical industry, medicine, agricultural chemicals.Owing to using and storing improper, hydrochloric ether is drained in physical environment by modes such as volatilization, leakage, discharge of wastewater.Large quantity research in recent years shows, the chlorinated hydrocarbon contaminants such as trieline have potential " three cause " (carcinogenic, teratogenesis, mutagenesis) effect and genetic toxic effect, liver, kidney and central nervous system injury can be caused as micro-trieline exposes contact, cause the disorderly symptom of a series of body function, seriously jeopardize human health.In Sustainable development, more and more higher requirement is proposed to ecotope along with international, domestic, how effectively to eliminate hydrochloric ether and pollute the important research content having become field of environment protection.
Consider from the angle solving pollution in wide area, biological degradation has high efficiency and low cost because of it, is considered to eliminate the most effective approach of chloro-alkenes pollutent.Biological degradation includes anaerobic and aerobic biological degradation two kinds of modes.Anaerobic degradation commonly uses the dechlorination bacterium such as Hyphomicrobium, Dehalococcoides, makes high chlorinatedorganic reduction dechlorination.Normal and the acetogen (homoacgtogens) of dechlorination bacterium and methanogen (methanogens) form consortium, under carbon source and exogenous electron donor existent condition, hydrochloric ether as electron acceptor(EA) for anaerobion provides the energy being degraded simultaneously.But there is the halfway defect of degraded in anaerobic degradation, the product after dechlorination often has larger bio-toxicity and carinogenicity.And aerobic degradation approach makes hydrochloric ether degradable by hydroxylation or epoxidation, relative to the halfway anaerobic biodegradation of degraded, aerobic biodegradation has significant advantage.
Methane-oxidizing bacteria (Methanotrophs) degradating chloro hydrocarbon belongs to the study hotspot in this field.The methane monooxygenase contained in methane-oxidizing bacteria can by epoxidised approach degradating chloro hydrocarbon, but because methane-oxidizing bacteria must realize the degraded of hydrochloric ether by the form of the Co metabolism adding carbon source, this limits the application of hydrochloric ether aerobic oxidation in engineering to a certain extent.
Summary of the invention
The object of this invention is to provide a kind of Methylobacterium of degradable hydrochloric ether, this bacterium can degradating chloro alkane and/or chloro-alkenes, can be applicable to the fields such as waste water, tap water and soil remediation, be specially adapted to chloro-alkenes contaminant degradation and soil organisms amendment in waste water, there is the meaning extensively promoted.
Technical scheme of the present invention is:
The Methylobacterium of degradable hydrochloric ether, its preserving number is that CCTCC NO:M 2015046 (is preserved in China typical culture collection center on January 18th, 2015, address: Wuhan University's preservation center, Luo Jia Shan, wuchang, wuhan), Classification And Nomenclature is: Methylobacterium sp.R1, and its 16S rDNA sequence is as shown in SEQ ID NO:1.
The Methylobacterium of degradable hydrochloric ether of the present invention, belongs to Methylobacter.Colony diameter 1 ~ 2mm, translucent, circular regular bacterium colony, low projection, neat in edge, homogeneous, surface is glossy, and color is many in yellow, is Gram-negative bacteria, without spore, amphitrichous, with one-sided raw polar flagellum motion, obligate aerobic, optimum growth temp is 28 ~ 30 DEG C, and pH is 7.0.
Methylobacterium of the present invention can degradating chloro alkane and/or chloro-alkenes.Wherein chloro-alkenes is selected from least one in trieline, Ethylene Dichloride, vinylchlorid, and chloroparaffin is selected from least one in tetrachloromethane, trichloromethane and ethylene dichloride.
The Methylobacterium of degradable hydrochloric ether of the present invention can be applicable to the fields such as waste water, tap water and soil remediation, can be used for biological catalyst.
The Methylobacterium of degradable hydrochloric ether of the present invention, called after Methylobacterium Methylobacterium sp.R1 in the application, be separated from household refuse landfill sites tectum and obtain, the order-checking of bacterial strain DNA entrusts the precious biotech firm in Dalian to complete, the length that the 16S rDNA base of Methylobacterium Methylobacterium sp.R1 measures is 1233bp (SEQ ID NO:1), base sequence compares at GenBank GenBank and finds and Methylobacterium Methylobacterium sp.SAP462, Methylobacterium sp.XJLW and Methylobacterium radiotolerans homology the highest, reach more than 97%.Shown by a series of Physiology and biochemistry of carrying out this bacterial strain and process optimization test, Methylobacterium Methylobacterium sp.R1 provided by the invention, can efficient degradation chloro-alkenes/chloroparaffin, major advantage is as follows:
(1) Methylobacterium Methylobacterium sp.R1 is with chloro-alkenes/chloroparaffin for carbon source and energy growth, overcomes methane-oxidizing bacteria and the Co metabolism approach of additional carbon must be relied on could to realize the limitation of chloro-alkenes/chloroparaffin degraded.
(2) use the intact cell of Methylobacterium Methylobacterium sp.R1 as biological catalyst, liquid culture cell density (under 600nm bacterium liquid absorbancy) can reach 1.2, still can maintain greater activity after departing from environmental system.
(3) this bacterium has height endurability to chloroform, can the chloroparaffin such as chloro-alkenes and tetrachloromethane, trichloromethane, ethylene dichloride such as efficient degradation trieline, Ethylene Dichloride, vinylchlorid.
(4) medium component needed for Methylobacterium Methylobacterium sp.R1 enlarged culturing is simple, and cost is low.Be specially adapted to chloro-alkenes contaminant degradation and soil organisms amendment in waste water, can extensively promote.
The invention solves aerobic biodegradation chlorinated hydrocarbon contaminants in prior art cannot the problem of through engineering approaches.
For above and other objects of the present invention, feature and advantage can be become apparent, under especially exemplified by preferred embodiment, and coordinate accompanying drawing, be described in detail below.
Accompanying drawing explanation
Fig. 1 is the stereoscan photograph of Methylobacterium Methylobacterium sp.R1;
Fig. 2 is the phylogenetic tree of Methylobacterium Methylobacterium sp.R1;
Fig. 3 is the growth curve of Methylobacterium Methylobacterium sp.R1.
Embodiment
Below in conjunction with embodiment, the invention will be further described, but embodiments of the present invention are not limited thereto.
1, experiment material
Table 1 Methylobacterium minimal medium composed as follows:
Nutritive substance Mass concentration (mg/L)
[0025]
KH 2PO 4 242
NaHPO 4.12H 2O 167
NH 4Cl 25
MgSO 4 68
CaCl 2 36
FeCl 3 0.25
CoCl 2.6H 2O 1.965
MnSO 4.H 2O 0.04
ZnSO 4.H 2O 0.04
All the other reagent are commercially available analytical pure product.
Granulated garbage is selected from household refuse landfill sites tectum, the present embodiment chooses the long-living bridge refuse landfill (water and soil runoff) in Chongqing, get 4mm and sieve granulated garbage on lower and 2mm sieve, add a certain amount of chloroform and regulate pH to being applicable to Methylobacterium growth, in methane and air Mixture, airtight domestication 2 weeks is to realize the rejuvenation of bacterial strain.
2. bacterial classification enrichment and optimization experiment
1) enrichment culture: take overburden soil 1g and put into 100ml minimal medium, is placed in 27 DEG C, 160 turns/min shaking table vibration 2h.Get bacteria suspension 2ml to access in the packing 100ml serum bottle of 20ml minimal medium as seed liquor, add a cover rubber stopper seal; Add 1ml trieline saturated solution, then 30 DEG C, shaking culture 1 week under 160 turns/min condition.
2) pure bacterium is separated: carry out 10 times of serial dilutions with the sterile distilled water cooled to bacterium liquid, making extent of dilution is 10 -1, 10 -2diluent, tilt-pour process is adopted to carry out culture medium culturing, flat-plate inverted is placed in vacuum drier, and a certain amount of trieline is passed in moisture eliminator, then with preservative film sealing, moisture eliminator is placed in biochemical cultivation case, cultivates 4 ~ 5d for 30 DEG C, the bacterial classification that growing way is good repeatedly goes down to posterity, purifying.
3) optimization experiment: utilize pure bacterial strain to prepare certain density bacteria suspension, join in the serum bottle that a certain amount of minimal medium is housed, adding concentration is tool plug sealing after the trieline of 20mg/L, 30 DEG C, shaking culture under 160 turns/min condition, certain interval of time detects bacterial concentration and trieline concentration.
3. strain identification experiment
QIAquick Genomic DNA Buffer Set is used to carry out pcr amplification object fragment.Get 5 μ l and carry out 3% agarose gel electrophoresis, use is cut glue recovery object fragment and is carried out DNA sequencing.The order-checking of DNA entrusts the precious biotech firm in Dalian to complete.DNA sequencing is carried out for primer with Seq Forward, Seq Reverse, Seq Internal.16S rRNA increases and adopts wide spectrum primers F 27 (SEQ ID NO:2-AGAGTTTGATCATGGCTCAG) and R1492 (SEQ ID NO:3-TACGGTTACCTTGTTACGACTT).
4. detection method
The OD value of bacterium liquid adopts UV2000 spectrophotometer to detect, and wavelength is 600nm.Viable cell concentrations adopts the method for plate culture count to determine.Dry cell weight is dried to constant weight by 10ml bacterium liquid at 80 DEG C, weighs by precision electronic balance.Each experiment is minimum does 2 ~ 3 groups of parallel tests, guarantees that RSD is less than 5%.
The detection of hydrochloric ether adopts gas-chromatography (Agilent 6890N).The stainless steel column of chromatographic condition: GDX (10m × 2mm), injector temperature, column temperature and detector (ECD) temperature are respectively 80,50,120 DEG C, and hydrogen is carrier gas, and flow velocity is 25ml/min, and sample size is 0.2ml.
The purifying of embodiment 1 Methylobacterium Methylobacterium sp.R1 and qualification
This Pseudomonas Gram-negative bacteria, colony diameter 1 ~ 2mm, translucent, circular regular bacterium colony, low projection, neat in edge, homogeneous, surface is glossy, and color is many in yellow; By stereoscan photograph known (see Fig. 1), Methylobacterium Methylobacterium sp.R1 is rod-short, diameter 0.6 ~ 1um, length 2 ~ 4um, without spore, and atrichia; Obligate aerobic, optimum growth temp is 28 ~ 30 DEG C, and pH is 7.0.
The order-checking of bacterial strain DNA entrusts the precious biotech firm in Dalian to complete, the length that the 16S rDNA base of Methylobacterium Methylobacterium sp.R1 measures is 1233bp (SEQ ID NO:1), it is the highest that base sequence compares discovery and Methylobacterium Methylobacterium sp.SAP462, Methylobacterium sp.XJLW and Methylobacterium radiotolerans homology at GenBank GenBank, reach more than 97%, can conclude that Methylobacterium Methylobacterium sp.R1 belongs to Methylobacter.
From phylogenetic tree (see Fig. 2), I type bacterium in Methylobacterium Methylobacterium sp.R1 and methane-oxidizing bacteria belongs to α-Proteobacteria, though Methylobacterium Methylobacterium sp.R1 is Methylobacterium (methyl bacterial genus), but current report is not classified as methane-oxidizing bacteria, and comprise Methylobacterium Methylobacter sp (also making methyl bacterial belong to) in methane-oxidizing bacteria classification.And known from phylogenetic tree, the sibship of the two is not very near, and Methylobacterium sp. is the G-bacillus having methylotrophy and methanotrophic characteristic concurrently, also can make carbon source and the energy with glucose or other complicated nutrition.
The growth characteristics of embodiment 2 Methylobacterium Methylobacterium sp.R1
Configuration glucose concn is the substratum 100ml of 2g/L, loads in 500ml serum bottle, and adds Methylobacterium bacterium liquid (OD 600nmbe 0.8) 3ml, builds plug and shakes up, put into shaking table and be set in 30 DEG C, cultivate under 160 turns/min condition, the OD of results of regular determination bacterium liquid 600nmvalue determines thalli growth situation.The growth curve of Methylobacterium Methylobacterium sp.R1 bacterial strain is shown in Fig. 3.The lag period of bacterial strain is approximately 25h.After entering logarithmic phase, thalli growth is rapid, and OD value rises to 1.2 and only used 15h, just reaches the stage of stable development afterwards, and it can glucose be that substratum obtains larger cell concentration that growth curve describes this bacterium.
Embodiment 3 Methylobacterium Methylobacterium sp.R1 tests different hydrochloric ether degradation effect
Configuration concentration is the trieline of 2g/L, Ethylene Dichloride, vinylchlorid, tetrachloromethane, trichloromethane and dichloroethane solution respectively, and respectively get in the 100ml serum bottle that 100 μ l add to respectively containing 20ml minimal medium, by above-mentioned minimal medium high-temp steam sterilizing under 121 DEG C of conditions, in bottle, add 1ml Methylobacterium bacterium liquid (OD with micropipet after cooling 600nmbe 0.8), build plug and shake up, put into shaking table and be set in 30 DEG C, cultivate 40h under 160 turns/min condition, then use the content of gas Chromatographic Determination hydrochloric ether, and determine its degradation effect, measure the OD of bacterium liquid 600nmvalue determines thalli growth situation.As shown in Table 2, Methylobacterium Methylobacterium sp.R1 can degrade these six kinds of hydrochloric ethers of trieline, Ethylene Dichloride, vinylchlorid, tetrachloromethane, trichloromethane and ethylene dichloride well, wherein best to the degradation effect of trieline, reach 88.9%, the degradation effect of ethylene dichloride is poor, is 28.7%.In general, the degradation effect of Methylobacterium Methylobacterium sp.R1 to chloro-alkenes is better than chloroparaffin.
Table 2Methylobacterium sp.R1 is to the degradation effect of hydrochloric ether
Vinylchlorid 37.4 +
Tetrachloromethane 55.0 ++
Trichloromethane 59.3 ++
Ethylene dichloride 28.7 +
The height endurability of Methylobacterium Methylobacterium sp.R1 to hydrochloric ether and the degradation characteristic of uniqueness thereof in the present invention, overcome the deficiency of co-metabolic degradation technique, but also can hydrochloric ether be sole carbon source and energy growth, be expected to obtain new breakthrough in the biodegradable engineer applied field of hydrochloric ether.
What more than enumerate is only a specific embodiment of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be had.All distortion that those of ordinary skill in the art can directly derive from content disclosed by the invention or associate, all should think protection scope of the present invention.

Claims (6)

1. a Methylobacterium for degradable hydrochloric ether, is characterized in that, its preserving number is CCTCC NO:M 2015046, and described Methylobacterium belongs to Methylobacter.
2. the Methylobacterium of degradable hydrochloric ether according to claim 1, is characterized in that, the 16S rDNA sequence of described Methylobacterium is as shown in SEQ ID NO:1.
3. the application of Methylobacterium in degradating chloro alkane and/or chloro-alkenes of degradable hydrochloric ether according to claim 1.
4. application according to claim 3, is characterized in that, described chloroparaffin is selected from least one in tetrachloromethane, trichloromethane, ethylene dichloride.
5. application according to claim 3, is characterized in that, described chloro-alkenes is selected from least one in trieline, Ethylene Dichloride, vinylchlorid.
6. the Methylobacterium of degradable hydrochloric ether according to claim 1 is preparing the application in biological catalyst.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115058362A (en) * 2022-06-23 2022-09-16 哈尔滨师范大学 Bacterial strain for degrading carbon tetrachloride and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5316940A (en) * 1992-04-24 1994-05-31 Board Of Regents, The University Of Texas System Constitutive soluble methane monooxygenase mutants of methanotrophic bacteria such as Methylosinus trichosporium A.T.C.C. 55314
CN101993839A (en) * 2010-07-23 2011-03-30 浙江工业大学 Methylobacterium rhodesianum H13 capable of efficiently degrading dichloromethane and application thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5316940A (en) * 1992-04-24 1994-05-31 Board Of Regents, The University Of Texas System Constitutive soluble methane monooxygenase mutants of methanotrophic bacteria such as Methylosinus trichosporium A.T.C.C. 55314
CN101993839A (en) * 2010-07-23 2011-03-30 浙江工业大学 Methylobacterium rhodesianum H13 capable of efficiently degrading dichloromethane and application thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
C. DEANE LITTLE ET AL.: "Trichloroethylene Biodegradation by a Methane-Oxidizing Bacterium", 《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》 *
V. C. FERNANDES ET AL.: "Dual augmentation for aerobic bioremediation of MTBE and TCE pollution in heavy metal-contaminated soil", 《BIODEGRADATION》 *
刘洪霞等: "二氯甲烷降解菌Methylobacterium rhodesianum H13 的分离鉴定及降解特性研究", 《环境科学》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115058362A (en) * 2022-06-23 2022-09-16 哈尔滨师范大学 Bacterial strain for degrading carbon tetrachloride and application thereof

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