CN104825632A - Drug for intervention treatment of diabetic peripheral neuropathy (DPN) - Google Patents
Drug for intervention treatment of diabetic peripheral neuropathy (DPN) Download PDFInfo
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- CN104825632A CN104825632A CN201510208679.8A CN201510208679A CN104825632A CN 104825632 A CN104825632 A CN 104825632A CN 201510208679 A CN201510208679 A CN 201510208679A CN 104825632 A CN104825632 A CN 104825632A
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Abstract
The invention discloses a drug for the intervention treatment of diabetic peripheral neuropathy (DPN). The drug comprises the following effective components in percentage by weight: 45 to 80% of chicken egg yolk, and 5 to 15% of dried rehmannia root. The results of animal experiments show that the provided drug can effectively reduce the blood sugar level of diabetic rats, moreover, the area under the curve (AUC) in the glucose tolerance tests is reduced, and the DPN pain index of diabetic rates is also reduced.
Description
Technical field
The present invention relates to the medicine of a kind of therapeutic intervention diabetic peripheral neuropathy pain, be specifically related to the medicine of the therapeutic intervention diabetic peripheral neuropathy containing Ovum Gallus domesticus Flavus and Radix Rehmanniae being effective ingredient.
Background technology
Diabetes (diabetes) be multiple virulence factor act on body cause hypoinsulinism, insulin resistant etc. and cause a series of metabolism disorder syndrome such as sugar, protein, fat, water and eletrolytes, be main feature clinically with hyperglycemia.The overall prevalence of the current diabetes of China reaches 9.7%.The same period prediabetes prevalence up to 15.5%.The reckoning made according to survey result shows China's total patient of diabetes patient number and reaches more than 9,000 2 hundred ten thousand, and prediabetes number reaches more than 100,000,000 4 thousand 8 hundred ten thousand.Diabetes be islet β cell insulin definitely or relative deficiency, companion or do not accompany insulin resistant.Onset diabetes process is slow, and mechanism is illustrated not yet completely.
The treatment of diabetes almost needs to run through lifelong, and treatment mainly contains insulin, chemicals and Chinese medicine etc., and other not yet full ripe method has islet transplantation and stem-cell therapy.Although method is many, all diabetes can not be cured, and the sickness rate of diabetes is in the situation that rises fast.Its main cause occurs in the process of development in diabetes, and islet beta cell function damage exists before onset diabetes, and this damage becomes irreversible along with increasing the weight of of degree.Unfortunately during onset diabetes, islet beta cell function damage is substantially in irreversible state, and the treatment of diabetes often just starts after diabetes occur.The current incurable main cause of diabetes that Here it is.Onset diabetes process is slow, what cause serious harm to diabetes patient's health and lives is the various chronic complicating diseases of this disease, comprising diabetic peripheral neuropathy, diabetes can damage all peripheral nerves, comprise acoustic nerve and the diabetic neuropathic dysaudia that arrives.Although because position is different during peripheral lesion, the clinical manifestation caused is different, it is then substantially identical to the mechanism of nerve injury.
There are some researches show that carrying out positive treatment to diabetes can effectively stop or the generation of delaying chronic complication.In this, Chinese medicine, through the practical application of several thousand, has been proved to be effective and rare side effect, can be used for the conditioning of body function, health-preserving functions such as health care and without medical ethics problems.Therefore Chinese medicine can play irreplaceable important function in the Primary preventive intervention treatment of diabetes.Fructus Mume processed is as a kind of Chinese medicine, sour in the mouth, micro-puckery, tool astringe the lung promote the production of body fluid, the merit of astringing intestine to stop diarrhea relieving colic caused by ascaris, due to energy exciting salivary secretion, promoting the production of body fluid to quench thirst, be commonly used to quench one's thirst (as the diabetes) for the treatment of thirsty polydipsia, in treatise on Febrile Diseases 326, just recite the treatment of wumei pills for " quenching one's thirst ", and improve the symptoms such as patient is easy hungry, thirsty, fatigue and weak.Containing abundant organic acid in Fructus Mume, as citric acid, malic acid, oxalic acid, glycolic, Fumaric acid etc., the mainly citric acid that wherein content is higher and malic acid; In addition, also the trace element such as chemical composition and Fe, Mg, Cu, Zn such as flavonoid, terpenoid, saccharide are contained in Fructus Mume.In experimentation in recent years, also from Fructus Mume, isolation identification has gone out 2 alkaloid compounds (2,2,6,6-tetramethylpiperidone, tert butyl urea), and superoxide dismutase (SOD); The vitamin content of Fructus Mume is also suitable abundant, and it contains the vitamin C approximate with other fruit and vitamin B1, but the content of vitamin B2 is hundreds of times more than of other fruit, reaches 5.6mg/100g.These abundant chemical compositions make Fructus Mume have the useful effect of heavy multipair human body.
Research display, in the control experiment of Fructus Mume and metformin, the glycemic control of Fructus Mume and metformin group is all right, the equal < 7.0mmol/L of fasting glucose, the equal < 10.0mmol/L of post-prandial glycemia; Glycolated hemoglobin (HbAlc) level of Fructus Mume group drops to 6.78% from 7.66%, the glycated hemoglobin levels of metformin group drops to 6.76% from 8.23%, no difference of science of statistics, describe Fructus Mume and can improve T2DM rat glycated hemoglobin levels, and there is obvious hypoglycemic activity.Meanwhile, the effect that Fructus Mume has reparation to impaired beta Cell of islet and regenerates, makes islet beta cell function recover and stimulates excreting insulin.
Ovum Gallus domesticus Flavus, Chinese medicine name.For the egg yolk of Phasianidae animal man chicken.Fresh egg shells, and removes albumen, stays egg yolk to use." book on Chinese herbal medicine is new again ": invigorating the spleen and replenishing QI, support kidney tonifying YIN, nourishing the lung to arrest cough, controls viscerasthenia hemoptysis.Modern study, the lecithin in egg yolk, triglyceride, cholesterol and Lututrin, have very large effect to nervous system and body development.Lecithin, by after human consumption, can discharge choline, and choline can improve the memory of each age group.Strong power is condensed and retain the whole nutrition of egg yolk, succession integration of edible and medicinal herbs theory, and with science and technology release egg yolk natural energy, the healthy and strong life of power-assisted, the Ovum Gallus domesticus Flavus instant egg yolk powder of research and development is also applicable to consumption by infants.Separately have research display, Ovum Gallus domesticus Flavus has blood sugar lowering, blood fat reducing and antioxidation; All there is from the isolated 25 kinds of polyphenolic substances of material extract the activity of significant Inhibiting α-glucosidase and scavenging free radicals, show Ovum Gallus domesticus Flavus and there is antioxidation.
Radix Rehmanniae, soil in taking a tonic or nourishing food to build up one's health, top grade for what nourish." herbal classic " main folding falls exhausted muscle person, and the justice of wound of namely enriching blood also.Main damaging the spleen and stomach person, i.e. its nourishing YIN merit of enriching blood.Abnormal smells from the patient peace, the deficiency of all internal organs, without non-availability, it nourishes.Main man's five kinds of strain and seven kinds of impairment of " not Lu ", woman's damaging the spleen and stomach, threatened abortion hematochezia, tonifying five ZANG-organs internal injury is not enough, all i.e. this purport.By arthralgia due to stagnation of blood person, then insufficiency of blood and numbness not all right, take a tonic or nourishing food to build up one's health sufficient, natural flow is permeated with, and numbness person's row.Fill out bone marrow, longue meat, then fill the meaning of its tonification and it is sayed in pole.Radix Rehmanniae has blood sugar lowering, blood fat reducing and antioxidation; All there is from the isolated 35 kinds of polyphenolic substances of Radix Rehmanniae extract the activity of significant Inhibiting α-glucosidase and scavenging free radicals, show Radix Rehmanniae and there is antioxidation.
Although show Ovum Gallus domesticus Flavus in above prior art and Radix Rehmanniae may have hypoglycemic effect respectively, but in prior art, do not provide the using method by Ovum Gallus domesticus Flavus and Radix Rehmanniae.Especially, because be rich in fructose in Ovum Gallus domesticus Flavus, when not understanding its mechanism and concrete effective ingredient, the fructose be rich in after taking without authorization will make the blood glucose generation rising sharply of diabetics, be unfavorable for the health of patient on the contrary.Moreover be rich in citric acid, malic acid and succinic acid in Radix Rehmanniae, polyphagia is damage tooth not only, also can damage taste.
In the prophylactic treatment stage of diabetes, it is one of problem proposed by the invention that the blood glucose how effectively controlling patient does not have side effects, meanwhile, in order to satisfied difference takes the needs of crowd, provide a kind of effective and cheap pharmaceutical composition also an object of the present invention.
Based on problem existing in prior art; the medicine that this patent is applied for is a kind of medicine with function of blood sugar reduction refined with pure natural medical Ovum Gallus domesticus Flavus and Radix Rehmanniae hybrid process; to the damage of prevention beta Cell of islet, protection islet beta cell function has special curative effect.Simultaneously, Ovum Gallus domesticus Flavus and Radix Rehmanniae compositions, by suitable proportioning, eliminate side effect when these two kinds of Chinese crude drugs are taken separately, as the medicine of antecedent intervention treatment diabetes, while achieving beyond thought therapeutic effect, conveniently also there is great practicality at cost and price.
Summary of the invention
Technical problem to be solved by this invention is as diabetes provided a kind of therapeutic intervention diabetic peripheral neuropathy medicine bitterly before morbidity.For solving the problem, the present inventor has found the medicine of a kind of therapeutic intervention diabetic peripheral neuropathy pain through concentrating on studies, it is characterized in that, be effective ingredient containing Ovum Gallus domesticus Flavus and Radix Rehmanniae.
The medicine of a kind of therapeutic intervention diabetic peripheral neuropathy pain of the present invention, the percentage ratio that preferred effective ingredient accounts for described pharmaceutical composition gross weight is: described Ovum Gallus domesticus Flavus accounts for 45% ~ 80%, and described Radix Rehmanniae accounts for 5% ~ 15%.
The medicine of a kind of therapeutic intervention diabetic peripheral neuropathy pain of the present invention, more preferably described Ovum Gallus domesticus Flavus accounts for 80%, and described Radix Rehmanniae accounts for 15%.
The medicine of a kind of therapeutic intervention diabetic peripheral neuropathy pain of the present invention, also comprises pharmaceutically acceptable carrier and/or excipient.
The present invention also provides the application in a kind of therapeutic intervention diabetic peripheral neuropathy pain, and it is characterized in that, described pharmaceutical composition contains Ovum Gallus domesticus Flavus and Radix Rehmanniae is effective ingredient.
The present invention also provides the application in a kind of therapeutic intervention diabetic peripheral neuropathy pain, and the percentage ratio that preferred described effective ingredient accounts for described pharmaceutical composition gross weight is: described Ovum Gallus domesticus Flavus accounts for 45% ~ 80%, and described Radix Rehmanniae accounts for 5% ~ 15%.
The present invention also provides the application of a kind of pharmaceutical composition in therapeutic intervention diabetic peripheral neuropathy pain, and more preferably described Ovum Gallus domesticus Flavus accounts for 80%, and described Radix Rehmanniae accounts for 15%.
Accompanying drawing explanation
Fig. 1: the distillate of embodiment of the present invention one example extracts figure.
Fig. 2: the present composition has the comparison diagram reducing blood glucose in diabetic rats effect.
Fig. 3: the present composition has the comparison diagram of the effect reducing diabetes rat glucose tolerance test area under curve.
Fig. 4: the present composition has the effect reducing experimental diabetic peripheral neuropathy in rats pain index
Detailed description of the invention
To embodiments of the present invention be illustrated below, however embodiments of the present invention not limit by following detailed description of the invention.
1. the separated and collected of Ovum Gallus domesticus Flavus and Radix Rehmanniae volatile oil
Volatile oil, has another name called quintessence oil, is the general name of the oily liquids not miscible with water that a class can obtain with vapor distillation.The common method extracting volatile oil from the fresh medical material or its quick freezing and cold preserving medical material of Ovum Gallus domesticus Flavus and Radix Rehmanniae has the way of distillation, solvent extraction method, milling process, supercritical fluid extraction method.Adopt the steam distillation that general traditional medicine volatile oil is taked in present embodiment, concrete grammar is conventional method, also can adopt the extracting method described in the patent documentations such as CN201940071U, CN2928228Y, CN2686690Y.
From fresh fruit, volatile oil is extracted except above-mentioned, can also be dry by various drying mode by raw material, then the conventional method adopted by this area prepares Chinese medical concrete, as long as when not destroying raw material main constituent, the optional main component extracting Ovum Gallus domesticus Flavus and Radix Rehmanniae in any way of the present invention, wherein preferable separate collects volatile oil, because sugar and other side effect composition can be reduced in rational scope in volatile oil.
2. the preparation of compositions
By the above-mentioned volatile oil of separated and collected from Ovum Gallus domesticus Flavus and Radix Rehmanniae respectively, mix according to specific ratio, compositions formulated stock solution.Also according to demands different clinically, make pill, powder, tablet, suppository, granule, membrane, capsule, microcapsule, drop pill, aerosol, injection, unguentum, medicated wine, syrup, oral solution, gel or liposome.Detailed description of the invention can perform in version standard according to Chinese Pharmacopoeia for 2000.
3. the effect detection of the present composition
3.1 animal origins and model preparation
Healthy male SD rat, in 2 months ages of Mus, body weight 150 ~ 200g, is provided by Zhejiang Province's Experimental Animal Center.The front animal of experiment is all placed in laboratory and conforms one week, freely drink water and standard rat chow nursing, keep clean in cage, room temperature controls at 23 ± 2 DEG C, natural lighting (supporting in University Of Ningbo's medical college Experimental Animal Center), relative humidity is 60% ~ 70%.6 rats also feed with normal diet surrounding as Normal group first random selecting.All the other rats high glucose and high fat feedstuff (15% Adeps Sus domestica, 20% sucrose, 13 yolk powders, 2% sodium cholate, 50% normal diet) is fed after surrounding with the dosage of 30mg/kg (1 week 1 time, continuous 2 weeks) disposable celiac injects 1% streptozotocin (STZ, Sigma Co., USA, lot number: S0130-1g) citrate buffer solution (with pH be 4.2 0.1mol/L sodium citrate buffer solution preparation, now with the current), Normal group synchronously injects the normal saline of equivalent in tail vein.
After two weeks, the rat of molding is cut tail and gets blood survey fasting glucose and random blood sugar, select fasting blood sugar (FBG) >=7.8mol/L, the rat of random blood sugar value >=11.1mol/L 12, and be divided into diabetic model group 6 at random, every day gavage 0.9% normal saline; Positive drug control group 6, every day gavage Fructus Mume Fructus Mori preparation.
3.2 intervening measure
Hop to it after diabetes model is successful pretreat.5g/kg dosage gastric infusion pressed by Ovum Gallus domesticus Flavus Radix Rehmanniae preparation, every day 1 time, continuous gavage 4 weeks.Normal group synchronously gives the normal saline of equivalent, continuous gavage 4 weeks.
Present composition experimental group according to the dosage gavage of 200ml/kg, the normal saline of Normal group and negative control group gavage equal solvent.Wherein when configuring compositions of the present invention, the remaining part outside Ovum Gallus domesticus Flavus and Radix Rehmanniae distillate is distilled water.Gavage interval is 12 hours.
The Acquire and process of 3.3 specimen
After treatment terminates, each group rat all surveys fasting glucose, weighs, carry out relative recording, then promptly rat is directly cut off side femoral artery and causes its death, then carries out dissection to rat and gets pancreas rapidly, 4 DEG C of normal saline flushings totally fix 24h with 4% formaldehyde, distilled water immersion 4h afterwards.Take out conventional gradients dehydration of alcohol, dimethylbenzene is transparent, paraffin embedding, LEICA2135 paraffin slicing machine serial section, and thickness 5 μm, gets 1 every 20, and each specimen gets 6, and mount respectively on the microscope slide scribbling polylysine, room temperature preservation is for subsequent use.
3.4 observation index
3.4.1 ordinary circumstance
Every day observes rats eating, drinking-water situation, urine volume, the changes such as mental status.Respectively before modeling, after modeling, after treatment, survey body weight.
3.4.2 blood sugar detection
3.4.2.1 fasting glucose and random blood sugar
Each treated animal is surveyed fasting glucose (FBG) (mmol/L) respectively before modeling, after modeling, after treatment, and surveyed a random blood sugar every 3 days, survey fasting glucose eve, Rat Fast 12h, next day, caudal vein got blood, measure fasting glucose by automatic blood glucose meter, and carry out relative recording.
3.4.2.2 oral glucose tolerance test (OGTT)
Row oral glucose tolerance test (OGTT) before modeling, after modeling, after treatment.After Rat Fast 12h, by 2g/kg dosage, by 50% glucose solution gavage, get tail vein in 0h, 0.5h, 1h, 2h and survey blood glucose.Experiment terminates rear drafting each group of rat glucose tolerance curve.
3.4.3 pathomorphology
Treatment terminates rear sacrifice of animal and wins pancreas rapidly, and after 4 DEG C of normal saline flushings are clean, 4% formaldehyde fixes 24h, distilled water immersion 4h.Conventional gradients dehydration of alcohol, dimethylbenzene is transparent, paraffin embedding, IEICA2135 paraffin slicing machine serial section, and thickness 5 μm, mounts on the microscope slide scribbling polylysine, uses the rear optical microphotograph Microscopic observation of hematoxylin-eosin staining method dyeing.
3.4.4 immunohistochemistry
Prepare the affine pure and mild antibody of HIF-1 α, instant SABC test kit, DAB colour reagent box.Get above-mentioned section to be placed in 60 DEG C of baking boxs and to examine sheet 1h, operate in strict accordance with test kit operating instruction, the conventional dewaxing of row section, distilled water immersion 2 minutes; Add 3% hydrogen peroxide at room temperature lower 10 minutes; Distillation washing 1 minute × 3 times; Section is placed in 0.01mol/L citrate buffer, and microwave heating is boiling extremely, power-off 15 minutes, again natural cooling after ebuillition of heated; Add 0.02mol/LPBS and wash 1 minute × 3 times; Add 5%BSA antigen blockade, lower 20 minutes of room temperature, gets rid of surplus liquid; Drip primary antibodie (1: 100) dilution, hatch 1 hour for 37 DEG C; 0.02mol/LPBS washes 2 minutes × 3 times; Drip biotinylation two to resist, hatch 20 minutes for 37 DEG C; 0.02mol/LPBS washes 2 minutes × 3 times; Drip the chain enzyme avidin of horseradish peroxidase-labeled, hatch 20 minutes for 37 DEG C; 0.02mol/LPBS washes 5 minutes × 4 times; DAB develops the color, and controls the response time under mirror; Haematoxylin redyes 10 seconds, tap water; Dehydration, transparent, resinene mounting.Light Microscopic observation, specific stain is lamellar brown yellow granule.The negative blank replacing primary antibodie with 0.1mol/LPBS liquid is all synchronously set up to often criticizing dyeing.Under optical microscope (400 times), often open section Stochastic choice 4 visuals field, count the positive cell number in each visual field respectively, finally compare with the mean of each group.
3.4.5RT-PCR
Concentration is surveyed after tissue extraction RNA, A1/A2 should between 1.8-2.0, by of short duration for RNA centrifugal after, add M-MLV reverse transcriptase 1ul, 5 × RT buffer 5ul, Oligo (dT) 18 (20mol/L) 2ul, dNTP (20umol/L) 2ul, RNA2ug, mend deionized water to 20ul volume, 42 DEG C of 1h reverse transcription reactions, 95 DEG C of 5min deactivation M-MIV, centrifugal again, 70 DEG C of heating 5min, stop above-mentioned reaction, be placed on ice, row reverse transcription reaction, strictly reaction reagent is added successively again by test kit operating instruction in PCR reaction tube, again PCR pipe is inserted PCR instrument, 95 DEG C of degeneration 5min, carry out 35 cyclic amplification reactions (94 DEG C, 1min, annealing temperature depending on primer (50-60 DEG C), 1min, 72 DEG C, 1min), then 72 DEG C of constant temperature 7min, take out PCR reaction tube, carry out electrophoresis detection to product, electrophoresis terminates rear taking-up agarose gel, is placed on gel imaging instrument lightly or imaging on ultraviolet transilluminator.Estimate the size of amplified band according to DNA molecular amount standard, electrophoresis result is formed e-file and file or take pictures by photographic system.
3.4.6Western blot
From tissue extraction albumen also quantitatively, after 10%SDS polyacrylamide gel electrophoresis protein isolate, electricity consumption is transferred on nitrocellulose filter, the complete rear title defatted milk powder TBST buffer of transferring film is made into the concentration se al of 5%, add primary antibodie (primary antibodie TBST is diluted to debita spissitudo) after washing film and hatch 1h, add after washing film once again and resist with two of Western bis-anti-diluted horseradish peroxidase (HRP) labelling and hatch 1h, with DAB nitrite ion colour developing 1h, with the molecular weight of gel image analysis systematic analysis object tape and clean on carry out optical density value.
Embodiment
Respectively the fresh fruit (or frozen fresh) of 1000g Ovum Gallus domesticus Flavus and Radix Rehmanniae is blended homogenate with blender, be placed in alembic as shown in Figure 1, after distillation, collect product of distillation.Wherein the product of distillation of Ovum Gallus domesticus Flavus is 643g, and yield is 64.3%, and the product of distillation of Radix Rehmanniae is 427g, and yield is 42.7%.
Above-mentioned product of distillation is carried out the formula of different proportion, and feed to rat model and normal rat in contrast, specific experiment is routine and comparative example is as shown in table 1.After surrounding administering transgenic terminates, add up the Evaluation of blood test value of each group of rat, with the concentration of determination of glucose oxidase serum glucose (unit is for mg/dl).
Fig. 2 display be the result of the present composition one model experiment example, each experimental group is the meansigma methods repeating for three times to test.Wherein,
A group is the blank of normal rat
B group is the blank of diabetic model rats
C group is the blood sugar concentration of diabetic model rats after present composition therapeutic intervention
D group is take separately the diabetic model rats of Ovum Gallus domesticus Flavus
E group is take separately the diabetic model rats of Radix Rehmanniae
Fig. 3 shows the medicine that this patent applies for the effect reducing diabetes rat glucose tolerance test area under curve, and in figure, a is normal group, b group, c group, d group and e group are respectively diabetes blank group and different medication group, and getting Normal group is 1, and all the other in contrast.Wherein,
A group is the blank of normal rat
B group is the blank of diabetic model rats
C group is the blood sugar concentration glycosuria of diabetic model rats after present composition therapeutic intervention
D group is take separately the diabetic model rats of Radix Rehmanniae
E group is take separately the diabetic model rats of Ovum Gallus domesticus Flavus
The result of other embodiments of the invention and reference examples is as shown in table 1, and according to result shown in table 1, after can learning the pharmaceutical composition using invention, the technique effect obtained has:
1., compared with matched group, the distillate of feed separately Fructus Mume processed or Fructus Mori, although have certain inhibitory action to hyperglycemia in diabetic model rats, the blood glucose of rat but can be reduced to lower level by compositions of the present invention.，
2., especially according to ratio of the present invention Fructus Mume processed used in combination and Fructus Mori, the blood sugar concentration of result display rat is controlled in the normal value between 7-10mg/dl, achieves beyond thought effect.
The blood sugar concentration of rat after 4 weeks in each experimental example of table 1. and reference examples
Claims (7)
1. a medicine for therapeutic intervention diabetic peripheral neuropathy pain, it is characterized in that, be effective ingredient containing Ovum Gallus domesticus Flavus and Radix Rehmanniae.
2. pharmaceutical composition as claimed in claim 1, it is characterized in that, the percentage ratio that described effective ingredient accounts for described pharmaceutical composition gross weight is: described Ovum Gallus domesticus Flavus accounts for 45% ~ 80%, and described Radix Rehmanniae accounts for 5% ~ 15%.
3. pharmaceutical composition as claimed in claim 2, it is characterized in that, described Ovum Gallus domesticus Flavus accounts for 80%, and described Radix Rehmanniae accounts for 15%.
4. the pharmaceutical composition as described in any one of Claims 1 to 4, is characterized in that, also comprises pharmaceutically acceptable carrier and/or excipient.
5. the application of pharmaceutical composition in therapeutic intervention diabetic peripheral neuropathy pain, it is characterized in that, described pharmaceutical composition contains Ovum Gallus domesticus Flavus and Radix Rehmanniae is effective ingredient.
6. apply as claimed in claim 5, it is characterized in that, the percentage ratio that described effective ingredient accounts for described pharmaceutical composition gross weight is: described Ovum Gallus domesticus Flavus accounts for 45% ~ 80%, and described Radix Rehmanniae accounts for 5% ~ 15%.
7. apply as claimed in claim 6, it is characterized in that, described Ovum Gallus domesticus Flavus accounts for 80%, and described Radix Rehmanniae accounts for 15%.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114410773A (en) * | 2022-01-27 | 2022-04-29 | 宁波大学 | Marker combination for predicting or diagnosing depression recurrence and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114410773A (en) * | 2022-01-27 | 2022-04-29 | 宁波大学 | Marker combination for predicting or diagnosing depression recurrence and application thereof |
| CN114410773B (en) * | 2022-01-27 | 2024-05-03 | 宁波大学 | Marker combination for predicting or diagnosing depression recurrence and application thereof |
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