CN104824670A - Preparation method of spartina alterniflora loisel fine powder and application of spartina alterniflora loisel fine powder to enhancement of organism immunity - Google Patents
Preparation method of spartina alterniflora loisel fine powder and application of spartina alterniflora loisel fine powder to enhancement of organism immunity Download PDFInfo
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- CN104824670A CN104824670A CN201510266409.2A CN201510266409A CN104824670A CN 104824670 A CN104824670 A CN 104824670A CN 201510266409 A CN201510266409 A CN 201510266409A CN 104824670 A CN104824670 A CN 104824670A
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- 239000000843 powder Substances 0.000 title claims abstract description 34
- 241001149258 Sporobolus alterniflorus Species 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 230000036039 immunity Effects 0.000 title claims abstract description 9
- 235000013305 food Nutrition 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims abstract description 5
- 229930003944 flavone Natural products 0.000 claims abstract description 4
- 235000011949 flavones Nutrition 0.000 claims abstract description 4
- 229930182490 saponin Natural products 0.000 claims abstract description 4
- 150000007949 saponins Chemical class 0.000 claims abstract description 4
- 238000001694 spray drying Methods 0.000 claims abstract description 4
- 238000005507 spraying Methods 0.000 claims abstract description 4
- 240000004928 Paspalum scrobiculatum Species 0.000 claims description 26
- 235000003675 Paspalum scrobiculatum Nutrition 0.000 claims description 26
- 229920002472 Starch Polymers 0.000 claims description 3
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 claims description 3
- 150000002212 flavone derivatives Chemical class 0.000 claims description 3
- 239000001397 quillaja saponaria molina bark Substances 0.000 claims description 3
- 239000002994 raw material Substances 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- 238000005728 strengthening Methods 0.000 claims description 3
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 claims description 3
- 238000003809 water extraction Methods 0.000 claims description 3
- 238000012360 testing method Methods 0.000 abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 5
- 241001465754 Metazoa Species 0.000 abstract description 4
- 241000196324 Embryophyta Species 0.000 abstract description 3
- 241000282414 Homo sapiens Species 0.000 abstract description 2
- 150000002213 flavones Chemical class 0.000 abstract 1
- 150000004676 glycans Chemical class 0.000 abstract 1
- 230000005484 gravity Effects 0.000 abstract 1
- 229920001282 polysaccharide Polymers 0.000 abstract 1
- 239000005017 polysaccharide Substances 0.000 abstract 1
- 235000017709 saponins Nutrition 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 description 9
- 102000016943 Muramidase Human genes 0.000 description 7
- 108010014251 Muramidase Proteins 0.000 description 7
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 7
- 229960000274 lysozyme Drugs 0.000 description 7
- 235000010335 lysozyme Nutrition 0.000 description 7
- 239000004325 lysozyme Substances 0.000 description 7
- 210000003296 saliva Anatomy 0.000 description 6
- 241000191967 Staphylococcus aureus Species 0.000 description 5
- 230000000242 pagocytic effect Effects 0.000 description 5
- 235000013361 beverage Nutrition 0.000 description 4
- 210000004493 neutrocyte Anatomy 0.000 description 4
- 210000000440 neutrophil Anatomy 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 230000003203 everyday effect Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 2
- 244000131316 Panax pseudoginseng Species 0.000 description 2
- 230000035622 drinking Effects 0.000 description 2
- 239000003651 drinking water Substances 0.000 description 2
- 235000020188 drinking water Nutrition 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 229960002897 heparin Drugs 0.000 description 2
- 229920000669 heparin Polymers 0.000 description 2
- 230000005180 public health Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 235000011496 sports drink Nutrition 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 244000020518 Carthamus tinctorius Species 0.000 description 1
- 235000003255 Carthamus tinctorius Nutrition 0.000 description 1
- 241000005787 Cistanche Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000515012 Micrococcus flavus Species 0.000 description 1
- 241001248610 Ophiocordyceps sinensis Species 0.000 description 1
- 235000003181 Panax pseudoginseng Nutrition 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 241001466077 Salina Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
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- 238000001962 electrophoresis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000002443 hepatoprotective effect Effects 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
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- 229910052760 oxygen Inorganic materials 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention belongs to the technical field of plant source function foods and particularly relates to a preparation method of spartina alterniflora loisel fine powder and application of the spartina alterniflora loisel fine powder to the enhancement of the organism immunity. The spartina alterniflora loisel fine powder is prepared by adding 20 times of hot water into ground parts of spartina alterniflora loisel which is harvested from October to December, and concentrating the solution to deep brown liquid with the specific gravity of 1.3. On the basis, a vacuum spraying dryer with a certain specification is selected and the temperature in a tank is adjusted to 170 DEG C; working parameters including a feeding flow and the like are automatically adjusted according to equipment requirements and equipment; and a finished product obtained by spray drying is slight yellow ultrafine powder with the size less than 200 meshes. A test shows that the content of total polysaccharides in the obtained spartina alterniflora loisel fine powder is more than or equal to 30%, the content of total saponins is more than or equal to 10% and the content of general flavones is more than or equal to 2.0%. An animal test and a population-based test verify that the product has the remarkable effect of improving the human immunity.
Description
Technical field
The invention belongs to the research and development field of plant source functional food.Relate generally to a kind of preparation method of Spartina alterniflora's extract and strengthening the application in immunity of organisms.
Background technology
In plant the content of bioactive ingredients and residing environmental concerns close.Content and its place of production degree of association of a lot of famous precious Chinese herbal medicine magistery are very high; Ginseng in picture China northeast mountain forest, the pseudo-ginseng of Southwest Mountainous Areas (especially showing with Yunnan), the Cordyceps sinensis, Xinjiang, the saline cistanche in psammophytes district, Inner Mongol, the safflower of high and cold mountain area, Tibet etc. of High Altitude in West China forest grassy marshland are enjoyed tremendous popularity in the world because magistery content under its special habitats condition is at source high
Spartina alterniflora is the halophytes grown in a kind of marine salina, be rich in various bioactivators, Nanjing University halophytes laboratory is development of raw materials new resource food " micro-many beverages " in the eighties in last century with Spartina alterniflora, obtain Ministry of Public Health's new resource food Health examination certification (defending new food No. 06th, accurate word-94) in August, 1994, on this basis, develop cooperatively " the composite rice grass oral liquid " with develop immunitypty and hepatoprotective effect with safe company of Jiangsu Taixing City, 1997 Nian Huo Ministry of Public Health health food certificates of approval (No. 321st, hygienic food and health number-1997), 2008, country defends " micro-many beverages " in " the new resource food list that approved is formally produced " of planning commission's announcement and ranks No. 27.Learn test test through repeatedly animal experiment and crowd, confirm that Spartina alterniflora and goods thereof have health-care efficacies such as well strengthening immunity of organisms, anti-ageing, antifatigue.In order to verify that Spartina alterniflora's extract rice grass fine powder strengthens effect of immunity of organisms, we devise and feed rat 28 days by rice grass fine powder, get the phagocytic rate of hematometry neutrophil cell to staphylococcus aureus; And organize crowd to learn test, aspiration examination takes rice grass fine powder 30 days, the change of detection experiment crowd lysozyme content, thus evaluates the effect that rice grass fine powder strengthens immunity of organisms.
Summary of the invention
1, the preparation of Spartina alterniflora's extract
Gather Spartina alterniflora's aerial part, dry, cut into 2 ~ 3cm length, add 20 times of hot water extraction, concentrated extracting solution is dark brown liquid, should reach following two indexs: proportion 1.3, dilutes 400 times of electrical conductivity >=1.0dS/m (25 ~ 28 DEG C).
2, the preparation of rice grass fine powder
Select the vacuum spray drying device of certain model, in regulating tank, temperature is 170 DEG C, the running parameters such as feed rate regulate automatically according to equipment requirement and equipment, there is provided Spartina alterniflora's extract up to standard to be raw material, spraying dry gained finished product is the faint yellow superfines of < 200 object.After testing, the rice grass fine powder total starches content that the present invention obtains is >=30%, and total saponin content is >=10%, and general flavone content is >=2.0%.
3, the preparation of rice grass fine powder application of samples
Take rice grass fine powder, be configured to the drinking water of 1: 400; Or tablet made by compressing tablet, every sheet, containing rice grass fine powder 0.3g, is tested for zoopery and crowd.
Specific embodiments
(1) preparation of rice grass fine powder
1, the preparation of Spartina alterniflora's extract
Gather Spartina alterniflora's aerial part 100kg, dry, cut into 2 ~ 3cm length, add 20 times of hot water extraction, concentrated extracting solution is dark brown liquid (about 10kg), following two indexs should be reached: proportion 1.3, dilute 400 times of electrical conductivity >=1.0dS/m (25 ~ 28 DEG C).
2, the preparation of rice grass fine powder
Select the vacuum spray drying device of certain model, in regulating tank, temperature is 170 DEG C, the running parameters such as feed rate regulate automatically according to equipment requirement and equipment, Spartina alterniflora's extract 10kg up to standard is provided, spraying dry gained 4kg finished product is the faint yellow superfines of < 200 object, i.e. rice grass fine powder.After measured, total starches content is >=30%, and total saponin content is >=10%, and general flavone content is >=2.0%.
3, the preparation of rice grass fine powder application of samples
Take rice grass fine powder 10g every day, be configured to the drinking water of 4000ml with pure water, freely drink for 6 cages (drinking bottle of every cage 500ml) rat (5, every cage), add not again.Get rice grass fine powder 1kg compressing tablet and make tablet, every sheet, containing rice grass fine powder 0.3g, is tested for crowd.
(2) efficacy test
Based on neutrophil cell, the phagocytic rate of staphylococcus aureus is tested
1, rat is cultivated and sampling for 28 days
There is provided about 220g body weight SD rat 30 by Nanjing Medical University's Experimental Animal Center, be divided into 3 groups at random, often organize 10, male and female half and half, single cage is raised.A, B, C three components Gei rat free drinking pure water every day, rice grass fine powder water (adding pure water 1: 400 configuration by rice grass fine powder) and certain sports drink domestic, the rat conventional feed that three treated animal ad libs every day are identical.After 28 days, 30 animal subject right eye socket of the eye medial canthals are taken a blood sample before test and to beverage (water).
2, detection experiment
Taking heparin anticoagulation 1.5ml (heparin concentration 10u/ml), 37 DEG C of standing 30min, treat blood cell natural subsidence, leukocytic plasma layer is rich in absorption, puts in vitro, adds equivalent staphylococcus aureus shake mixing, 37 DEG C of water-bath 25min, abandon supernatant after centrifugal, sediment is carefully beaten, get 1 and push into thin slice, air oxygen detrition, Giemsa-Wright dyes, and counts 100 neutrophil leucocytes under oily mirror, calculates the phagocytic rate (P R) of neutrophil leucocyte:
P R=engulfs neutrophil leucocyte number/100 neutrophil leucocyte × 100% of bacterium
Record testing result, in table 1.
The phagocytic rate (%) of rat neutrophil cell to staphylococcus aureus of different beverage (water) drunk by table 1
Note: * * shows P < 0.01.
This test proves, drink the meaning that the rat neutrophil cell raising to staphylococcus aureus phagocytic rate of rice grass fine powder water after 28 days has highly significant, its effect exceedes certain sports drink domestic.
Based on edible rice grass fine powder, test is learned to the crowd that lysozyme content changes
1, the sheet test of rice grass fine powder and lysozyme sampling is taken
Select 48 Nanjing university for the aged aspiration persons of participating in the experiment, the age, men and women half and half at 55 years old-65 years old, was divided into two large groups, often organized 24 people (men and women half and half); One group of per day for human beings takes rice grass fine powder sheet 2, and another group is not taken, and contrasts, test period 30 days.Every person of participating in the experiment before participating in the experiment and off-test time carry out the collection of saliva: by clean tube, the saliva that nature flows out is collected to every the person of participating in the experiment, gathers after the person of participating in the experiment of individual acquisition difficulty stimulates lower jaw gland to impel salivary secretion with disinfecting cotton swab.
2, detection experiment
Saliva all gathers the morning 8 ~ 10 time, and measures complete with agarose rocket electrophoresis the same day in collection, and the lysozyme standard product of this research build up Bioengineering Research Institute by Nanjing to be provided, and micrococcus flavus is cultivated by this room.Saliva lysozyme active testing the results are shown in Table 2.
The change (μ g/ml) of crowd's saliva lysozyme activity of rice grass fine powder taken by table 2
Note: * * shows P < 0.01.
This test proves, take the elderly population of rice grass fine powder sheet after 30 days, the raising of the content of its saliva lysozyme has the meaning of highly significant.
Claims (3)
1. the preparation method of Spartina alterniflora's extract, it is characterized in that: annual October-December gathers in the crops Spartina alterniflora's aerial part, dry, be cut to 2 ~ 3cm long, add 20 times of hot water extraction, concentrated extracting solution is dark brown liquid, should reach following two indexs: proportion 1.3, dilutes 400 times of electrical conductivity >=1.0dS/m (25 ~ 28 DEG C).
2. the preparation method of meter careless fine powder, it is characterized in that: the vacuum spray drying device selecting certain model, in regulating tank, temperature is 170 DEG C, the running parameters such as feed rate regulate automatically according to equipment requirement and equipment, there is provided the Spartina alterniflora's extract meeting claims 1 feature to be raw material, spraying dry gained finished product is the faint yellow superfines of < 200 object.Its total starches content is >=30%, and total saponin content is >=10%, and general flavone content is >=2.0%.
3. Spartina alterniflora's extract described in claim 1 and 2 and rice grass fine powder are strengthening the application in body immunity function food.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510266409.2A CN104824670A (en) | 2015-05-20 | 2015-05-20 | Preparation method of spartina alterniflora loisel fine powder and application of spartina alterniflora loisel fine powder to enhancement of organism immunity |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510266409.2A CN104824670A (en) | 2015-05-20 | 2015-05-20 | Preparation method of spartina alterniflora loisel fine powder and application of spartina alterniflora loisel fine powder to enhancement of organism immunity |
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| CN104824670A true CN104824670A (en) | 2015-08-12 |
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| CN201510266409.2A Pending CN104824670A (en) | 2015-05-20 | 2015-05-20 | Preparation method of spartina alterniflora loisel fine powder and application of spartina alterniflora loisel fine powder to enhancement of organism immunity |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107551167A (en) * | 2017-10-12 | 2018-01-09 | 南京壹唯壹生物科技有限公司 | Purposes of Spartina alterniflora's extract in blood uric acid functional product is prepared |
| CN107603926A (en) * | 2017-11-10 | 2018-01-19 | 丁爱军 | The method that Bifidobacterium liquid is cultivated using Spartina alterniflora |
| CN109534984A (en) * | 2018-12-19 | 2019-03-29 | 南京大学 | A method of p-Coumaric Acid is prepared using Spartina alterniflora |
| CN115606733A (en) * | 2022-08-29 | 2023-01-17 | 信阳农林学院 | Natural food preservative and preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6087547A (en) * | 1999-05-25 | 2000-07-11 | University Of South Carolina | Method for decomposing toxic organic pollutants |
| CN1849908A (en) * | 2006-05-31 | 2006-10-25 | 南京大学 | Method for controlling smooth cord-grass by deep developing and utilizing it |
| CN1860238A (en) * | 2003-10-31 | 2006-11-08 | 雷克斯基因生物科技有限公司 | Method for preparing extract of sophorae fructus containing high concentration of aglycone by hot water extraction |
| CN101019606A (en) * | 2006-12-26 | 2007-08-22 | 南京大学 | Production process and application of composite cord grass coloring agent for feed |
| CN104396569A (en) * | 2014-12-10 | 2015-03-11 | 福建农林大学 | Method for recycling spartina alterniflora |
-
2015
- 2015-05-20 CN CN201510266409.2A patent/CN104824670A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6087547A (en) * | 1999-05-25 | 2000-07-11 | University Of South Carolina | Method for decomposing toxic organic pollutants |
| CN1860238A (en) * | 2003-10-31 | 2006-11-08 | 雷克斯基因生物科技有限公司 | Method for preparing extract of sophorae fructus containing high concentration of aglycone by hot water extraction |
| CN1849908A (en) * | 2006-05-31 | 2006-10-25 | 南京大学 | Method for controlling smooth cord-grass by deep developing and utilizing it |
| CN101019606A (en) * | 2006-12-26 | 2007-08-22 | 南京大学 | Production process and application of composite cord grass coloring agent for feed |
| CN104396569A (en) * | 2014-12-10 | 2015-03-11 | 福建农林大学 | Method for recycling spartina alterniflora |
Non-Patent Citations (4)
| Title |
|---|
| 姜允申等: "一种生物矿质食品添加剂的保健作用", 《中华预防医学杂志》 * |
| 胡芝华等: "互花米草总黄酮降血脂作用研究", 《海洋科学》 * |
| 覃佐东等: "简析互花米草（Spartina alterniflora)的研发态势与综合利用", 《科技通讯》 * |
| 钦佩等: "互花米草的营养成分", 《中国药学杂志》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107551167A (en) * | 2017-10-12 | 2018-01-09 | 南京壹唯壹生物科技有限公司 | Purposes of Spartina alterniflora's extract in blood uric acid functional product is prepared |
| CN107551167B (en) * | 2017-10-12 | 2020-12-22 | 南京施倍泰生物科技有限公司 | Application of spartina alterniflora extract in preparation of functional product for reducing blood uric acid |
| CN107603926A (en) * | 2017-11-10 | 2018-01-19 | 丁爱军 | The method that Bifidobacterium liquid is cultivated using Spartina alterniflora |
| CN109534984A (en) * | 2018-12-19 | 2019-03-29 | 南京大学 | A method of p-Coumaric Acid is prepared using Spartina alterniflora |
| CN115606733A (en) * | 2022-08-29 | 2023-01-17 | 信阳农林学院 | Natural food preservative and preparation method and application thereof |
| CN115606733B (en) * | 2022-08-29 | 2023-11-17 | 信阳农林学院 | Natural food preservative and preparation method and application thereof |
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