CN104824662A - Lactic acid bacteria composition capable of regulating intestinal flora functions and preparation method and application thereof - Google Patents
Lactic acid bacteria composition capable of regulating intestinal flora functions and preparation method and application thereof Download PDFInfo
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/175—Rhamnosus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/249—Thermophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/533—Longum
Abstract
The invention discloses a lactic acid bacteria composition capable of regulating intestinal flora functions and preparation method and application thereof. The active ingredients of the lactic acid bacteria composition capable of regulating intestinal flora functions are prepared from the following raw materials by weight: 20-40 parts of Lactobacillus acidophilus, 40-60 parts of Bifidobacterium longum, 20-60 parts of Lactobacillus rhamnosus, 30-60 parts of Streptococcus thermophilus and 35-75 parts of fructo-oligosaccharide. According to the lactic acid bacteria composition capable of regulating intestinal flora functions, lactic acid bacteria in the composition can reach a certain magnitude, and the raw materials are in reasonable compatibility, and have a good function of regulating intestinal flora. The lactic acid bacteria composition has good stability in a long time, and is suitable for the diabetics to eat.
Description
Technical field
The present invention relates to field of health care food, particularly relate to lactic bacteria composition of a kind of tool regulating intestinal canal flora function and preparation method thereof and application.
Background technology
Colony balance and intestinal health closely bound up.Gut flora comprises beneficial bacterium and harmful bacteria.In healthy human body, beneficial bacterium is preponderated, and interacts with harmful bacteria, jointly maintains the balance of Tiny ecosystem in intestines, maintains health.If make the probio in enteron aisle reduce because of various factors impact, harmful bacteria amount reproduction, in intestines, microecological balance is broken, and will cause the various clinical symptoms such as diarrhoea.Usually can use broad-spectrum antibiotic in a large number clinically, although can effective kill harmful bacterium, also can kill beneficial bacterium simultaneously, cause intestinal bacilli illness, cause disease.
For intestinal bacilli illness person, per os probiotic supplemented preparation is the way of directly effectively regulating intestinal canal flora.It directly or indirectly can adjust the composition of host intestine microorganism, and activation host endogenous microbes group or immune activity realize prebiotic effect.
Although probiotic food has many benefits to health, but manufacturer asks to adapt to the taste that consumer is afraid of acid, and manufactured goods all with the addition of a large amount of sugars, and fruity Yoghourt carrys out seasoning with flavoring essence.In addition, also there is shelf-life short, the shortcoming such as storage cost of transportation is high in the liquid form product such as lactobacillus yogurt.And, kinds of lactobacillus is various, preferably suitable bacterial classification also carries out reasonably combined to reach the difficult point that better health-care effect is also this area now, therefore develop that a kind of strain collocation is reasonable, nutrition good, sugar content is low, long shelf-life, steady quality, and the lactic bacteria composition with good health-care effect is necessary.
Summary of the invention
Based on this, be necessary for the problems referred to above, provide lactic bacteria composition of a kind of tool regulating intestinal canal flora function and preparation method thereof and application, lactic bacteria composition strain collocation of the present invention is reasonable, nutrition good, sugar content is low, long shelf-life, steady quality, and there is good health-care effect.
For realizing above-mentioned technical purpose, concrete technical scheme is as follows:
A lactic bacteria composition for tool regulating intestinal canal flora function, its active component is prepared from by the raw material of following weight portion: lactobacillus acidophilus 20 ~ 40 parts, bifidobacterium longum 40 ~ 60 parts, Lactobacillus rhamnosus 20 ~ 60 parts, streptococcus thermophilus 30 ~ 60 parts, FOS 35 ~ 75 parts.
Inventor team is according to technical purpose of the present invention, in conjunction with the functional characteristic such as the security of all types of probio, field planting and adhesive force, antibacterial, acidproof and bile tolerance ability, by a large amount of research and experiments, probio is screened, finally preferred Lactobacillus rhamnosus, bifidobacterium longum, lactobacillus acidophilus, streptococcus thermophilus.Lactobacillus and Bifidobacterium are the important physiology bacterium of human intestine, to the physiological action that body has other normal physiological bacterium multiple incomparable, comprising: regulating intestinal canal flora, strengthen host intestine resistance, prevention and therapy diarrhoea etc.Lactobacillus acidophilus and Lactobacillus rhamnosus belong to lactobacillus, and the former is present in the small intestine site of very strictly anaerobic environment, have stronger acidproof and resistance to bile ability, can are colonizated in large intestine smoothly by gastrointestinal environment; And Lactobacillus rhamnosus can grow very well under anaerobic conditions, enteron aisle Stickiness is higher, and colonization ability is strong, and the ability of its resistance to intestinal environment is better than common sour milk bacterial classification and other probio bacterial classification, plays the effect such as regulating intestinal canal flora, prevention and therapy diarrhoea; Streptococcus thermophilus, as potential beneficial bacterium, can arrive the first half of small intestine, has certain intestines and stomach adhesiveness and transport activity; Bifidobacterium longum is present in large intestines, can adhere to host's intestinal epithelial cell surface, form stable flora, play biological barrier and Ant agonism.Bifidobacterium longum repels pathogenic bacteria by self and the metabolite that produces, and in enteron aisle, keep flora advantage, and interact with other flora, the relation between adjustment flora, and maintains colony balance.
There is intergrowth relation in lactobacillus acidophilus preferred for this invention, Lactobacillus rhamnosus, streptococcus thermophilus, bifidobacterium longum, need acetate, vitamin B2 and folic acid etc. as nutriment in the growth of lactobacillus acidophilus, and bifidobacterium longum in fermentation except generating penta acetic acid, can also the multivitamin such as synthetic vitamin B1, B2, B12 and folic acid, just supply the needs of lactobacillus acidophilus, promote the growing multiplication of lactobacillus acidophilus.Lactobacillus acidophilus grows soon in micro-oxygen containing newborn matrix, progressively can reduce the oxidation-reduction potential in newborn matrix, and provide the material of various decomposed easy utilization, promotes the growth of bifidobacterium longum; Lactobacillus rhamnosus homofermentation, produce Pfansteihl, can ferment multiple sugar, promotes the growth of the beneficial bacterium such as bifidobacterium longum and lactobacillus; When lactobacillus acidophilus and streptococcus thermophilus mixed culture fermentation, compensate for streptococcus thermophilus because of the more weak and defect in the nutrition caused of the ability of decomposing protein, accelerate the growth of streptococcus thermophilus; Lactic acid, formic acid etc. that simultaneously streptococcus thermophilus metabolism produces are the growth stimulants of lactobacillus acidophilus, can play facilitation in various degree to lactobacillus acidophilus.FOS is as the prebiotics of a kind of Be very effective, high-quality, lactobacillus acidophilus can be made at lower doses, bifidobacterium longum is multiplied, much lower compared with its utility scale of other compound sugar, but effect is more obvious, and FOS water activity is lower compared with other prebioticses, can better be composite with probio, ensure that probio keeps active within the longer shelf-life.The compatibility of lactobacillus acidophilus, Lactobacillus rhamnosus, streptococcus thermophilus, bifidobacterium longum and FOS is rational, formula is while probiotic supplemented and prebiotics, increase intestines peristalsis, bacterium excessively increases in control, regulating intestinal canal flora and the normal pH value of recovery enteron aisle.
Wherein in some embodiments, its active component is prepared from by the raw material of following weight portion: lactobacillus acidophilus 25 ~ 35 parts, bifidobacterium longum 45 ~ 55 parts, Lactobacillus rhamnosus 35 ~ 45 parts, streptococcus thermophilus 35 ~ 45 parts, FOS 45 ~ 65 parts.
Wherein in some embodiments, its active component is prepared from by the raw material of following weight portion: lactobacillus acidophilus 29 ~ 31 parts, bifidobacterium longum 49 ~ 51 parts, Lactobacillus rhamnosus 39 ~ 41 parts, streptococcus thermophilus 39 ~ 41 parts, FOS 54 ~ 56 parts.
The present invention also provides a kind of preparation method of lactic bacteria composition of above-mentioned tool regulating intestinal canal flora function, comprises the steps:
1) take raw material according to above-mentioned proportioning, described raw material is lactobacillus acidophilus, bifidobacterium longum, Lactobacillus rhamnosus, streptococcus thermophilus, FOS;
2) by step 1) gained raw material mixes with auxiliary material acceptable on health food, sieves, mix.
Wherein in some embodiments, on described health food, acceptable auxiliary material is maltitol 35 ~ 65 parts, maltodextrin 30 ~ 70 parts, flavoring essence 5 ~ 15 parts.
Wherein in some embodiments, on described health food, acceptable auxiliary material is maltitol 51 ~ 53 parts, maltodextrin 49 ~ 51 parts, flavoring essence 9 ~ 11 parts.
The lactic bacteria composition that present invention also offers above-mentioned tool regulating intestinal canal flora function is preparing the application in health food.
Present invention also offers a kind of health food, described health food includes the lactic bacteria composition of above-mentioned tool regulating intestinal canal flora function.
The present invention compared to the advantage of prior art and beneficial effect is:
(1) the present invention through inventor team for a long time for the research of lactic acid bacteria, and a large amount of experiments and contrast test, preferably comprise lactobacillus acidophilus, bifidobacterium longum, Lactobacillus rhamnosus, the raw material components of streptococcus thermophilus and FOS, and preferred optimum proportioning, above-mentioned raw materials component cooperatively interacts, have and regulate body composition of gut flora, keep microecological balance, recover the functions such as the normal pH value of enteron aisle, the present invention can make lactic acid bacteria in lactic bacteria composition reach some levels, each material combination is reasonable, there is good regulating intestinal canal flora function, gained lactic bacteria composition good stability in a long time, and be applicable to diabetes patient.
(2) preparation method of the present invention is easy and simple to handle, reliable, productivity effect is high, is applicable to the large production of industry.
Detailed description of the invention
Raw materials usedly in embodiment be commercially available convenient source.
Use in following examples and comparative example lactobacillus acidophilus, bifidobacterium longum, Lactobacillus rhamnosus, streptococcus thermophilus, lactobacillus bulgaricus, bifidobacterium lactis is all purchased from Jin Qiao bio tech ltd, Anhui.
Below in conjunction with specific embodiment, set forth the present invention further.
Embodiment 1
A lactic bacteria composition for tool regulating intestinal canal flora function, it is prepared from by following raw material:
Lactobacillus acidophilus 20 parts, bifidobacterium longum 60 parts, Lactobacillus rhamnosus 60 parts, streptococcus thermophilus 30 parts, FOS 75 parts, flavoring essence 15 parts, maltitol 35 parts, maltodextrin 70 parts.
The preparation method of the lactic bacteria composition of above-mentioned tool regulating intestinal canal flora function comprises the steps:
1) lactobacillus acidophilus, bifidobacterium longum, Lactobacillus rhamnosus, streptococcus thermophilus, FOS is taken according to said ratio;
2) by step 1) each raw material mix with flavoring essence, maltitol, maltodextrin, cross 50 mesh sieves, be placed in total mixed machine, mix;
3) by step 2) products obtained therefrom is checked, packing: inspect by random samples by control standard in enterprise product, after the assay was approved, and packing, packing specification: 5g/ wraps;
4) extract point product installed on request, test by target level of product quality;
5) after the assay was approved, after quality department provides verification of conformity, outsourcing, warehouse-in.
Embodiment 2
A lactic bacteria composition for tool regulating intestinal canal flora function, it is prepared from by following raw material:
Lactobacillus acidophilus 40 parts, bifidobacterium longum 40 parts, Lactobacillus rhamnosus 20 parts, streptococcus thermophilus 60 parts, FOS 35 parts, flavoring essence 5 parts, maltitol 65 parts, maltodextrin 30 parts.
Embodiment 2 preparation method and embodiment 1 are together.
Embodiment 3
A lactic bacteria composition for tool regulating intestinal canal flora function, it is prepared from by following raw material:
Lactobacillus acidophilus 30 parts, bifidobacterium longum 50 parts, Lactobacillus rhamnosus 40 parts, streptococcus thermophilus 40 parts, FOS 55 parts, flavoring essence 10 parts, maltitol 52 parts, maltodextrin 50 parts.
Embodiment 3 preparation method and embodiment 1 are together.
Embodiment 4
It is below the quality standard for lactic bacteria composition that inventor and team thereof formulate according to food security GB etc.
Table 1 lactic bacteria composition quality standard Q/SYSW0003S-2011
Embodiment 5
The microorganism of the lactic bacteria composition of the tool regulating intestinal canal flora function obtained by embodiment 3 and sanitary index detect
Detect every microorganism of the lactic bacteria composition of the embodiment of the present invention 3 tool regulating intestinal canal flora function according to " GB 7101-2003 solid beverage sanitary standard " and " lactic bacteria composition quality standard Q/SYSW0003S-2011 " and chemical index as follows:
The lactic bacteria composition microorganism of table 2 embodiment 3 tool regulating intestinal canal flora function and sanitary index
Embodiment 6
The lactic bacteria composition stability test result of the tool regulating intestinal canal flora function obtained by embodiment 3
Stability sense organ: sample (obtains the lactic bacteria composition of the tool regulating intestinal canal flora function of three batches again according to embodiment 3 technical scheme, be respectively 513120803,513120804,513120805) be white powder, there is the distinctive smell of this product, free from extraneous odour, without naked eyes visible foreign; Sample deposits 18 months under nature (normal temperature) condition, and the organoleptic indicators such as the color of product, proterties, smell and quality are all normal, without significant change occurs.Micro biological Tests result and physical and chemical inspection result are specifically in table 3 and table 4.
The lactic bacteria composition stability experiment testing result (depositing 0 month) of table 3 embodiment of the present invention 3 tool regulating intestinal canal flora function
Check-Out Time is: on August 12 ,-2012 years on the 5th August in 2012
The lactic bacteria composition stability experiment testing result (after depositing 18 months) of table 4 embodiment of the present invention 3 tool regulating intestinal canal flora function
Check-Out Time is: on February 12 ,-2014 years on the 5th February in 2014
According to examination by sensory organs result, Micro biological Tests result, the physical and chemical inspection result of the said goods stability test, the lactic bacteria composition that the present invention has regulating intestinal canal flora function deposits 18 months under nature (normal temperature) condition, the inherent quality index of product and sanitary index meet product relevant criterion, point out this constant product quality.
Embodiment 7
The lactic bacteria composition acute toxicity test of the tool regulating intestinal canal flora function obtained by embodiment 3
Test method: select body weight 18-22g healthy NIH kind small white mouse 20, male and female half and half.Adopt maximum tolerance measurement Law, if tested material preparation is 20.0g/kg.BW, take sample (lactic bacteria composition of the tool regulating intestinal canal flora function obtained by embodiment 3) during test, every 20.0g pure water is diluted to 30ml for examination.Fasting can't help water after 16 hours, and once, gavage amount is 0.3ml/10g.BW to gavage, observes one week, record mouse poisoning manifestations and death condition.
Result of the test and discussion: observe per os gavage after one week, do not observe animal and occur bad reaction, according to acute toxicity classification, the lactic bacteria composition of tested material tool of the present invention regulating intestinal canal flora function belongs to nontoxic level material.
Embodiment 8 human experimentation method
8.1 experimenter's mass selections are selected in nearly one month and were not suffered from gastrointestinal disease, do not take antibiotic, age 20-65 year, health check-up index is normal volunteer 90 all.
Volunteer is divided into experimental group and control group 1, control group 2 with grouping by 8.2 dosage at random, and often organize 30 people, men and women half and half.
8.3 experimental technique experimental group take the lactic bacteria composition of tool regulating intestinal canal flora function obtained by embodiment 3; Control group 1, control group 2 take comparative example 1, comparative example 2 (concrete formula asks for an interview following comparative example 1,2) preparation respectively.Test group and control group instructions of taking are every day 1 time, each 1 bag, take 15 days continuously.Be responsible for sending out sample by special messenger and supervised and take, duration of test does not change original eating habit, normal diet.Test group and each control group are before taking tested material and take tested material continuously after 15 days, aseptic collection ight soil carries out 10 times of serial dilutions respectively, select suitable dilution factor, be seeded on various culture medium respectively, cultivate on request, then bacterium colony (enterobacteriaceae lactobacteriaceae (enterobacteria), enterococcus, Bifidobacterium, lactobacillus, bacteroid and aerogenesis folder film bacillus) counting is carried out to culture.
Comparative example 1,2 is filled a prescription as follows:
Comparative example 1
A kind of lactic bacteria composition, its preparation method is substantially the same manner as Example 3, and difference is also to contain bifidobacterium lactis, lactobacillus bulgaricus in its formula, not containing Lactobacillus rhamnosus, is specially:
Lactobacillus acidophilus 35 parts, bifidobacterium longum 55 parts, lactobacillus bulgaricus 35 parts, streptococcus thermophilus 30 parts, bifidobacterium lactis 35 parts, FOS 55 parts, flavoring essence 10 parts, maltitol 52 parts, maltodextrin 50 parts.
Comparative example 2
A kind of lactic bacteria composition, its preparation method is substantially the same manner as Example 3, and difference is also to contain bifidobacterium lactis in its formula, not containing Lactobacillus rhamnosus, streptococcus thermophilus, is specially:
Lactobacillus acidophilus 40 parts, bifidobacterium longum 55 parts, bifidobacterium lactis 45 parts, FOS 55 parts, flavoring essence 10 parts, maltitol 52 parts, maltodextrin 50 parts.
8.4 experimental results and analytical table
Table 5 human experiment group foretastes front and back flora situation and compares (cfu/g)
As shown in Table 5, compared with control group 1, control group 2, experimental group crowd is after the lactic bacteria composition of the tool regulating intestinal canal flora function obtained by edible embodiment 3, the quantity of Bifidobacterium and lactobacillus significantly increases, and enterobacteria, enterococcus, bacteroid and C.perfringens quantity all decrease.
Human test results: from table 5, after the lactic bacteria composition of edible tool regulating intestinal canal flora function obtained by embodiment 3, experimental group lactobacillus and Bifidobacterium are accelerated and are significantly higher than control group, and enterobacteria, enterococcus, bacteroid and C.perfringens all decrease before edible.
Above-mentioned human trial shows: the lactic bacteria composition of tool regulating intestinal canal flora function of the present invention has the effect of regulating intestinal canal colony balance.
Each technical characteristic of the above embodiment can combine arbitrarily, for making description succinct, the all possible combination of each technical characteristic in above-described embodiment is not all described, but, as long as the combination of these technical characteristics does not exist contradiction, be all considered to be the scope that this description is recorded.
The above embodiment only have expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but can not therefore be construed as limiting the scope of the patent.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (8)
1. the lactic bacteria composition of a tool regulating intestinal canal flora function, it is characterized in that, its active component is prepared from by the raw material of following weight portion: lactobacillus acidophilus 20 ~ 40 parts, bifidobacterium longum 40 ~ 60 parts, Lactobacillus rhamnosus 20 ~ 60 parts, streptococcus thermophilus 30 ~ 60 parts, FOS 35 ~ 75 parts.
2. the lactic bacteria composition of tool regulating intestinal canal flora function according to claim 1, it is characterized in that, its active component is prepared from by the raw material of following weight portion: lactobacillus acidophilus 25 ~ 35 parts, bifidobacterium longum 45 ~ 55 parts, Lactobacillus rhamnosus 35 ~ 45 parts, streptococcus thermophilus 35 ~ 45 parts, FOS 45 ~ 65 parts.
3. the lactic bacteria composition of tool regulating intestinal canal flora function according to claim 2, it is characterized in that, its active component is prepared from by the raw material of following weight portion: lactobacillus acidophilus 29 ~ 31 parts, bifidobacterium longum 49 ~ 51 parts, Lactobacillus rhamnosus 39 ~ 41 parts, streptococcus thermophilus 39 ~ 41 parts, FOS 54 ~ 56 parts.
4. a preparation method for the lactic bacteria composition of the tool regulating intestinal canal flora function described in any one of claims 1 to 3, is characterized in that, comprise the steps:
1) take raw material according to the proportioning described in any one of claims 1 to 3, described raw material is lactobacillus acidophilus, bifidobacterium longum, Lactobacillus rhamnosus, streptococcus thermophilus, FOS;
2) by step 1) gained raw material mixes with auxiliary material acceptable on health food, sieves, mix.
5. preparation method according to claim 4, is characterized in that, on described health food, acceptable auxiliary material is maltitol 35 ~ 65 parts, maltodextrin 30 ~ 70 parts, flavoring essence 5 ~ 15 parts.
6. preparation method according to claim 5, is characterized in that, on described health food, acceptable auxiliary material is maltitol 51 ~ 53 parts, maltodextrin 49 ~ 51 parts, flavoring essence 9 ~ 11 parts.
7. the lactic bacteria composition of the tool regulating intestinal canal flora function as described in any one of claims 1 to 3 is preparing the application in health food.
8. a health food, is characterized in that, described health food includes the lactic bacteria composition of the tool regulating intestinal canal flora function as described in any one of claims 1 to 3.
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