CN104822843B - 使用人干细胞样细胞和代谢组学比率预测药物的人发育毒性 - Google Patents

使用人干细胞样细胞和代谢组学比率预测药物的人发育毒性 Download PDF

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CN104822843B
CN104822843B CN201380057328.0A CN201380057328A CN104822843B CN 104822843 B CN104822843 B CN 104822843B CN 201380057328 A CN201380057328 A CN 201380057328A CN 104822843 B CN104822843 B CN 104822843B
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test compound
teratogenicity
ratio
cells
fold change
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CN104822843A (zh
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A.史密斯
P.韦斯特
J.帕尔默
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Shi Te Meter Na Biomarker Research And Development Co
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Shi Te Meter Na Biomarker Research And Development Co
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6806—Determination of free amino acids
    • G01N33/6812—Assays for specific amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6806—Determination of free amino acids
    • G01N33/6812—Assays for specific amino acids
    • G01N33/6815—Assays for specific amino acids containing sulfur, e.g. cysteine, cystine, methionine, homocysteine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N23/00—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
    • G01N23/22—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material
    • G01N23/225—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material using electron or ion
    • G01N23/2255—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material using electron or ion using incident ion beams, e.g. proton beams
    • G01N23/2258—Measuring secondary ion emission, e.g. secondary ion mass spectrometry [SIMS]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
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  • Toxicology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN201380057328.0A 2012-11-02 2013-11-01 使用人干细胞样细胞和代谢组学比率预测药物的人发育毒性 Active CN104822843B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261721746P 2012-11-02 2012-11-02
US61/721746 2012-11-02
US201361827407P 2013-05-24 2013-05-24
US61/827407 2013-05-24
PCT/US2013/067980 WO2014071137A1 (en) 2012-11-02 2013-11-01 Predicting human developmental toxicity of pharmaceuticals using human stem-like cells and metabolomic ratios

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CN104822843B true CN104822843B (zh) 2020-01-21

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US (3) US20150301025A1 (https=)
EP (1) EP2914748B1 (https=)
JP (2) JP6339581B2 (https=)
CN (1) CN104822843B (https=)
CA (1) CA2888064C (https=)
DK (1) DK2914748T3 (https=)
HU (1) HUE043604T2 (https=)
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US10060932B2 (en) * 2013-07-09 2018-08-28 Stemina Biomarker Discovery, Inc. Biomarkers of autism spectrum disorder
FI126398B (en) 2014-12-18 2016-11-15 Upm Kymmene Corp Method for chemical testing
US10767164B2 (en) 2017-03-30 2020-09-08 The Research Foundation For The State University Of New York Microenvironments for self-assembly of islet organoids from stem cells differentiation
CN109207551B (zh) * 2017-07-07 2023-05-26 上海睿智化学研究有限公司 一种筛选叶酸代谢相关药物的方法
WO2019173671A1 (en) * 2018-03-09 2019-09-12 Stemina Biomarker Discovery, Inc. In vitro assay to predict cardiotoxicity
US12548652B2 (en) 2020-11-30 2026-02-10 Sanofi Classifying images of dose-response graphs
CN119667130B (zh) * 2025-02-13 2025-05-09 生态环境部华南环境科学研究所(生态环境部生态环境应急研究所) 一种基于血清组氨酸/犬尿氨酸快速评估双酚类物质暴露毒性效应的方法

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US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US7329489B2 (en) 2000-04-14 2008-02-12 Matabolon, Inc. Methods for drug discovery, disease treatment, and diagnosis using metabolomics
US20060258672A1 (en) * 2005-05-13 2006-11-16 Joseph Barbosa Multicyclic compounds and methods of their use
JP5421767B2 (ja) * 2006-04-10 2014-02-19 ウィスコンシン・アルムニ・リサーチ・ファウンデーション ヒト胚性幹細胞を使用して薬学的化合物および他の化学物質の毒性を評価するための試薬および方法
JP5814337B2 (ja) * 2010-03-22 2015-11-17 ステミナ バイオマーカー ディスカバリー， インコーポレイテッド ヒト幹細胞様細胞及びメタボロミクスを使用した医薬のヒト発生毒性の予測
US20120071543A1 (en) * 2010-05-14 2012-03-22 Carter Jacob L Formulations of diluted genetic material and methods for making same
US9447506B2 (en) 2012-07-30 2016-09-20 David Whitmore Cathodic protection of a concrete structure

Non-Patent Citations (1)

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Title
Identifying developmental toxicity pathway for a subset of ToxCast chemical using human embryonic stem cells and metabolomics;N.C. Kleinstreuer,et al.;《Toxicology and Applied Pharmacology》;20111231;第257卷;112页左栏第3段 *

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Publication number Publication date
CN104822843A (zh) 2015-08-05
US20180128814A1 (en) 2018-05-10
EP2914748B1 (en) 2019-03-13
MX2015005476A (es) 2015-10-29
JP6581244B2 (ja) 2019-09-25
JP6339581B2 (ja) 2018-06-06
US10209240B2 (en) 2019-02-19
US20150301025A1 (en) 2015-10-22
CA2888064A1 (en) 2014-05-08
WO2014071137A1 (en) 2014-05-08
US20190128872A1 (en) 2019-05-02
EP2914748A1 (en) 2015-09-09
MX362991B (es) 2019-03-01
JP2016501516A (ja) 2016-01-21
EP2914748A4 (en) 2016-06-01
DK2914748T3 (da) 2019-06-11
CA2888064C (en) 2023-05-30
HUE043604T2 (hu) 2019-08-28
JP2018157823A (ja) 2018-10-11

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