CN104813877A - Application of heterorhabditis beicherriana in prevention and control of batocera horsfieldi on walnut trees - Google Patents
Application of heterorhabditis beicherriana in prevention and control of batocera horsfieldi on walnut trees Download PDFInfo
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- CN104813877A CN104813877A CN201510275633.8A CN201510275633A CN104813877A CN 104813877 A CN104813877 A CN 104813877A CN 201510275633 A CN201510275633 A CN 201510275633A CN 104813877 A CN104813877 A CN 104813877A
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- heterorhabditis
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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Abstract
The invention relates to application of heterorhabditis beicherriana in prevention and treatment of batocera horsfieldi on walnut trees. The heterorhabditis beicherriana is capable of preventing and controlling trunk boring pests. The heterorhabditis beicherriana is prepared into preparations which are applied to a walnut garden; the test result shows that the preparations have remarkable effects in prevention and control of batocera horsfieldi eggs and larvae; prevention and control effect for larvae is up to 90% (correction efficiency is 91.8%) and effect of preventing egg hatching is close to 70% (67.7%).
Description
Technical field
The present invention relates to technical field of biological control, a kind of new heterorhabditis indica Heterorhabditis beicherriana particularly utilize a kind of patent of the present invention first applicant to gather, to be separated, identifying is for field control walnut batocera horsfieldi.
Background technology
Batocera horsfieldi (Batocera horsfieldi) is also known as Batocera horsfieldi, belong to coleoptera (Coleoptera) Cerambycidae (Cerambycidae), be the great forest trunk borer of China, walnut endanger particularly serious.Mainly take food the harm of brill moth with larva at trunk xylem, and survive the winter in burrow He in pupa room with larva and adult, form a large amount of burrow, time serious, cause tree death or windbreak, thus make it to lose economic worth and the ecological value.
For a long time for this class pest control mainly by artificial hook kill with burrow in inject high concentration, high toxicity chemical pesticide.But these method effects are unsatisfactory, and to dispenser personnel and ecotope toxicity larger.
Heterorhabditis indica was found in 1975 and names, it only comprises different little bar and belongs to Heterorhabditis mono-genus, this genus nematode has described 9 kinds at present, wherein cherry heterorhabditis indica Heterorhabditis beicherriana is China Agricultural University, insect and nematology laboratory, patent first applicant of the present invention gathers from Shunyi, Beijing cherry orchard, be separated, a novel species (Li Xingyue of qualification, Liu Qizhi*, Nermut J., Puza, V.and Mracek is beicherrianan.sp. (Nematoda:Heterorhabditidae) Z.2012.Heterorhabditis, a new entomopathogenic nematode from the Shunyi districtof Beijing, China.Zootaxa, 3569:25-40.), there is not yet the report with this newfound nematoda control walnut batocera horsfieldi up to now both at home and abroad.
Summary of the invention
The present invention is directed to the blank in above-mentioned field, the application of cherry heterorhabditis indica Heterorhabditis beicherriana in control walnut batocera horsfieldi is provided, to solve the difficult problem in production, widens biological control approach.
The application of cherry heterorhabditis indica Heterorhabditis beicherriana in control trunk borer.
Described trunk borer is longicorn.
Described longicorn is walnut batocera horsfieldi.
Described application be by described cherry heterorhabditis indica preparation grow up develop of plant impose on endangered by longicorn trunk, branch cutting portion and defecation hole in.
Described plant is walnut, and described longicorn is walnut batocera horsfieldi.
Described control suppresses batocera horsfieldi egg hatching or kills batocera horsfieldi larva or adult.
Described cherry heterorhabditis indica preparation is the aqueous suspension of cherry heterorhabditis indica, and concentration is 250-8000 bar/hole, and amount of application is 1-4mL/ channel or cutting.
The concentration of described cherry heterorhabditis indica preparation is 2000-4000 bar/mL.
The invention provides a kind of new entomopathogenic nematode preparation, the cherry heterorhabditis indica Heterorhabditis beicherriana adopting patent first applicant of the present invention to collect from the cherry orchard soil of Shunyi, Beijing makes preparation, be applied in Hetaoyuan, result shows that the effect of this nematoda control walnut batocera horsfieldi ovum and larva is all very remarkable, control larva effect reaches more than 90% (correcting efficient 91.8%), stops egg hatching effect nearly 70% (67.7%).
Accompanying drawing explanation
The correction of each dosage of Fig. 1 (concentration) cherry nematode H.beicherriana to walnut batocera horsfieldi larva is efficient
Embodiment
Now the present invention is described in further detail in conjunction with the embodiments.
Although the embodiment of the present invention is just applied to the control of walnut batocera horsfieldi, but concerning harm other fruit trees and other trunk borer (as flatheaded borer, bark beetle etc.), based on same principle, cherry heterorhabditis indica is equally applicable to orchard and the forest land of the harm of other longicorn and trunk borer harm.Have only unlike practical technique, both the Harm period of different trunk borer on different regions, different host was different with the position depth, need adjust according to biological property.
Embodiment one, cherry heterorhabditis indica H.beicherriana prevent and treat the field efficacy case of walnut batocera horsfieldi ovum
1 materials and methods
1.1 test material
Reagent agent: cherry heterorhabditis indica Heterorhabditis beicherriana, this first inventor collection from the cherry orchard soil of Shunyi, Beijing, separation, purifying, the numerous gained of expansion, can external disclosure granting.Nematode after collection suspends in aqueous, 4 DEG C of preservations, uses in 20 days.Nematode application concentration is as following test method.
1.2 test method
The 7-9 month in 2014 by the Hetaoyuan of batocera horsfieldi serious harm, does not carry out other control at wild horse Chuan Zhen Su Ga village, Hezhang County, Guizhou Province random selecting.Walnut is marked with the new cutting of longicorn ovum.
Cherry heterorhabditis indica H.beicherriana is made into respectively 250IJs/mL, 500IJs/mL, 1000IJs/mL, 2000IJs/mL and 4000IJs/mL five concentration gradients.Each process arranges 3 repetitions (i.e. 3 walnuts), and the cutting number 20 ~ 100 of each repetition (on every walnut) not etc., does not mark all cuttings.Each cutting top layer spreads the nematode viscous fluid of a certain concentration of 1-2mL.Equivalent clear water viscous fluid is used in contrast.Within every 7 days, observe and remove worm excrement after recording the cutting number once having excrement to get rid of, observing 7 times altogether, to the 49th day.
1.3 computational methods
By following formulae discovery:
The efficient %=of ovum is without worm excrement rate %=[1-(defecation cutting number/total cutting number)] × 100%
Correct efficient %=(processing without the rate-contrast of worm excrement without worm excrement rate)/(100%-contrast is without worm excrement rate) × 100%
2 results and analysis
2.1 cherry nematode fields stop the effect of walnut batocera horsfieldi egg hatching
As seen from Table 1: use the cherry heterorhabditis indica H.beicherriana the 49th day for examination, the correction of walnut batocera horsfieldi egg hatching is efficient the highest under 2000IJs/mL nematode dosage, reaches 67.7%.From overall trend, the nematode infection time is longer, and hatching inhibiting rate is higher, stops egg hatching to go out the efficient higher of larva.
Table 1 cherry heterorhabditis indica Heterorhabditis beicherriana stops the correction efficient (%) of batocera horsfieldi egg hatching
Embodiment two, cherry heterorhabditis indica H.beicherriana prevent and treat the field efficacy case of walnut batocera horsfieldi larva
1 materials and methods
1.1 test material
Reagent agent: cherry heterorhabditis indica Heterorhabditis beicherriana, this first inventor collection from the cherry orchard soil of Shunyi, Beijing, separation, purifying, the numerous gained of expansion, can external disclosure granting.Nematode after collection suspends in aqueous, 4 DEG C of preservations, uses in 20 days.Nematode application concentration is as following test method.
1.2 test method
The 7-9 month in 2014 by the Hetaoyuan of batocera horsfieldi serious harm, does not carry out other control at wild horse Chuan Zhen Su Ga village, Hezhang County, Guizhou Province random selecting.Walnut is marked with the new defecation hole of roundheaded borer.
Cherry heterorhabditis indica H.beicherriana is made into respectively 250IJs/mL, 500IJs/mL, 1000IJs/mL, 2000IJs/mL and 4000IJs/mL five concentration gradients.Each process arranges 3 repetitions (i.e. 3 walnuts), and the defecation hole count 20 ~ 100 of each repetition (on every walnut) not etc., does not mark all defecation holes.The nematode viscous fluid of a certain concentration of 2-4mL is injected in each defecation hole.Equivalent clear water viscous fluid is used in contrast.Within every 7 days, observe and remove worm excrement after recording the defecation hole once having excrement to get rid of, observing 4 times altogether, to the 28th day.
1.3 computational methods
By following formulae discovery:
The efficient %=of larva is without worm excrement rate %=[1-(defecation hole count/total hole count)] × 100%
Correct efficient %=(processing without the rate-contrast of worm excrement without worm excrement rate)/(100%-contrast is without worm excrement rate) × 100%
2 results and analysis
2.1 cherry nematode field control walnut batocera horsfieldi larvas efficient
As seen from Table 2: the cherry heterorhabditis indica H.beicherriana for examination has very high infecting potential to walnut batocera horsfieldi larva, 7-28 days efficient reaches 77.9 ~ 94.0%.Use 2000IJs/mL dosage, 7 days time control efficiency nearly 90% (89.2%), efficient more than 90 (92.2) % when 14 days, when the 28th, efficiently reaches peak (94.0%).
It is efficient that table 2 Heterorhabditis beicherriana infects walnut batocera horsfieldi larva
The correction of 2.2 cherry nematode field control walnut batocera horsfieldi larvas is efficient
As can be seen from Figure 1: infect walnut batocera horsfieldi larva the 28th day for examination cherry heterorhabditis indica H.beicherriana, it corrects and efficiently assigns 85.9% at 1000IJs/mL nematode dosage; 91.8% is assigned at 2000IJs/mL nematode dosage; 88.4% is assigned at 4000IJs/mL nematode dosage.
From overall trend, nematode is being infected under 28 days time spans, and the infective dose of 2000IJs/mL is best, best to the control efficiency of larva, efficient the highest.
Claims (8)
1. the application of cherry heterorhabditis indica Heterorhabditis beicherriana in control trunk borer.
2. application according to claim 1, described trunk borer is longicorn.
3. application according to claim 2, described longicorn is walnut batocera horsfieldi.
4. application according to claim 2, described application be by described cherry heterorhabditis indica preparation grow up develop of plant impose on endangered by longicorn trunk, branch cutting portion and defecation hole in.
5. application according to claim 4, described plant is walnut, and described longicorn is walnut batocera horsfieldi.
6. application according to claim 5, described control suppresses batocera horsfieldi egg hatching or kills batocera horsfieldi larva or adult.
7. application according to claim 6, described cherry heterorhabditis indica preparation is the aqueous suspension of cherry heterorhabditis indica, and concentration is 250-8000 bar/hole, and amount of application is 1-4mL/ channel or cutting.
8. application according to claim 7, the concentration of described cherry heterorhabditis indica preparation is 2000-4000 bar/mL.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510275633.8A CN104813877A (en) | 2015-05-26 | 2015-05-26 | Application of heterorhabditis beicherriana in prevention and control of batocera horsfieldi on walnut trees |
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| CN201510275633.8A CN104813877A (en) | 2015-05-26 | 2015-05-26 | Application of heterorhabditis beicherriana in prevention and control of batocera horsfieldi on walnut trees |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105053020A (en) * | 2015-08-10 | 2015-11-18 | 中国农业大学 | Application of H.beicherriana in preventing and treating lissorhoptrus oryzophilus kuschel |
| CN106818327A (en) * | 2017-02-13 | 2017-06-13 | 贵州省林业科学研究院 | A kind of walnut batocera horsfieldi larva prevention and controls |
| CN109452313A (en) * | 2018-12-07 | 2019-03-12 | 四川省农业科学院植物保护研究所 | A kind of entomopathogenic nematode preparation and preparation method and application |
-
2015
- 2015-05-26 CN CN201510275633.8A patent/CN104813877A/en active Pending
Non-Patent Citations (6)
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| 刘奇志等: "《昆虫线虫抑制植物线虫的机理研究进展》", 《植物保护》 * |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105053020A (en) * | 2015-08-10 | 2015-11-18 | 中国农业大学 | Application of H.beicherriana in preventing and treating lissorhoptrus oryzophilus kuschel |
| CN105053020B (en) * | 2015-08-10 | 2018-05-29 | 中国农业大学 | Application of the cherry heterorhabditis indica in rice water weevil is prevented |
| CN106818327A (en) * | 2017-02-13 | 2017-06-13 | 贵州省林业科学研究院 | A kind of walnut batocera horsfieldi larva prevention and controls |
| CN109452313A (en) * | 2018-12-07 | 2019-03-12 | 四川省农业科学院植物保护研究所 | A kind of entomopathogenic nematode preparation and preparation method and application |
| CN109452313B (en) * | 2018-12-07 | 2020-09-22 | 四川省农业科学院植物保护研究所 | Entomopathogenic nematode preparation, preparation method and application |
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Application publication date: 20150805 |