CN104807948A - Quality control method for anti-vertigo granules - Google Patents
Quality control method for anti-vertigo granules Download PDFInfo
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- CN104807948A CN104807948A CN201510235587.9A CN201510235587A CN104807948A CN 104807948 A CN104807948 A CN 104807948A CN 201510235587 A CN201510235587 A CN 201510235587A CN 104807948 A CN104807948 A CN 104807948A
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Abstract
The invention discloses a quality control method for anti-vertigo granules. The method comprises a ligusticum wallichii identifying method, a salvia miltiorrhiza identifying method and a puerarin content measuring method. The quality control method for the anti-vertigo granules provides the methods for identifying ligusticum wallichii and salvia miltiorrhiza in the medicine, and provides an efficient liquid chromatography method for measuring the content of the puerarin ingredients in the medicine, the quality control method can be used for better controlling the medicine quality and has the advantages of high specificity and good reproducibility, and safety, effectiveness and quality controllability of the medicine are really realized.
Description
Technical field
The invention belongs to Control of drug quality field, be specifically related to a kind of method of quality control of combating vertigo particle.
Background technology
Vertigo is clinical common sympton, relates generally to the multidisciplinary diseases such as ear nose larynx, cardiovascular, neural, backbone.This sick clinical onset is anxious, with ictal dizziness, have a headache, look eye is fuzzy, Nausea and vomiting for main clinical manifestation, have a strong impact on the work of patient, life.The vertigo incidence of disease is about 5%, and along with the arrival of aging society, the incidence of disease of this disease is in rising trend.Vertigo sends out well crowd with artificially master in old age, and within more than 60 years old, the elderly accounts for this sick incidence of disease about 70%, and women is more than the male sex.The pathologic classifications of vertigo comprises patients with vestibular system vertigo and peripheral vertigo.Dissimilar vertigo pathogenic factor and involved histoorgan are different, and the disease causing vertigo according to statistics has kind more than 50, relate to each histoorgan of whole body.
Modern medicine thinks that its factors such as generation and cerebral microcirculation disturbance, Abnormal Blood Rheology, intracellular free radicals damage has substantial connection.Cerebral blood supply insufficiency belongs in traditional Chinese medicine " dizzy " category." Ling Shu Miraculous Pivot or Divine Axis sea opinion " is said: " the brain being the reservoir of the marrow ".Li Shizhen (1518-1593 A.D.) is said: " the brain being the house of mentality ".The elderly's internal organs function declines, healthy tendency virtual loss, and the deficiency of vital energy then blood is not all right and be the stasis of blood, and blood is not up, and brain is lost and filled, and sends out as dizzy.The object of this research is, by pharmacy, pharmacology and inquire into anti-dizzy particle to the curative effect of cerebral blood supply insufficiency and bad reaction situation thereof by observing clinical symptoms, TCD (transcranial Doppler) etc.Guarantee that invention formulation quality carries out qualitative and quantitative analysis to effective constituent in its ingredient.
Summary of the invention
An object of the present invention is the method for quality control for providing the dizzy particle of a kind of anti-blooming, and the method can differentiate Ligusticum wallichii in the dizzy particle of anti-blooming and the red sage root fast and accurately, and by the content of Puerarin composition in high effective liquid chromatography for measuring combating vertigo particle.
The invention provides a kind of method of quality control of combating vertigo particle, comprise the content assaying method of Ligusticum wallichii discrimination method, red sage root discrimination method and Puerarin.
Further, described Ligusticum wallichii discrimination method is:
Get combating vertigo particle porphyrize, get 3g, add diethyl ether 50ml, ultrasonic 20 minutes, and filter, filtrate volatilizes, and the residue after volatilizing adds methenyl choloride 1ml, dissolved residue, as need testing solution;
Separately get Ligusticum wallichii control medicinal material 1g, add diethyl ether 20ml, is made in the same way of control medicinal material solution;
Get again not containing the negative control sample of Ligusticum wallichii, be made in the same way of negative control solution;
According to thin-layered chromatography test, draw each 5 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, with the normal hexane-ethyl acetate of volume ratio 9:1 for developping agent, launch, taking-up is dried, and inspects under putting ultraviolet lamp 365nm;
Observe in test sample chromatogram, on the position corresponding to control medicinal material chromatogram, the fluorescence spot of aobvious same color.
Further, described red sage root discrimination method is:
Get combating vertigo particle porphyrize, get 3g, add the ultrasonic 30min of 75% methyl alcohol 20ml, filter, filtrate volatilizes, and the residue after volatilizing adds 75% methyl alcohol 1ml, and dissolved residue, as need testing solution;
Separately get protocatechualdehyde reference substance appropriate, make the reference substance solution of 1mg/ml;
Separately get red sage root control medicinal material 1g, be made in the same way of control medicinal material solution;
Get again not containing the negative control sample of the red sage root, be made in the same way of negative control solution;
According to thin-layered chromatography test, draw each 5 μ l of above-mentioned four kinds of solution, put respectively on same silica gel g thin-layer plate, take volume ratio as the toluene-methenyl choloride-acetate-methanol-formic acid of 2:3:4:0.5:2 be developping agent, launch, taking-up is dried, and inspects under putting uviol lamp 254nm;
Observe in test sample chromatogram, on the position corresponding to reference substance chromatogram, the spot of aobvious same color.
Further, the content assaying method of described Puerarin is for adopting high effective liquid chromatography for measuring:
Chromatographic condition and system suitability take octadecylsilane chemically bonded silica as filling agent; Take volume ratio as methyl alcohol-0.1% phosphoric acid solution of 25:75 be mobile phase; High voltage and constant current wash-out, flow velocity 1.0ml/min; Determined wavelength is 250nm, and number of theoretical plate calculates by puerarin peak and answers >=1000;
The preparation of reference substance solution, gets Puerarin reference substance appropriate, accurately weighedly puts in measuring bottle, adds 30% ethanol and makes the solution of every 1ml containing 80 μ g, to obtain final product;
The preparation of need testing solution, gets combating vertigo particle appropriate, porphyrize, get about 2.0g, accurately weighed, put in tool plug conical flask, precision adds 30% ethanol 50ml, close plug, weighed weight, ultrasonic process, lets cool, more weighed weight, supply the weight of less loss with 30% ethanol, shake up, get supernatant, filter, get subsequent filtrate, to obtain final product;
Assay method: accurate absorption reference substance solution and each 20 μ l of need testing solution respectively, injection liquid chromatography, measures, to obtain final product.
Further, described ultrasonic process is power 160W, frequency 50kHz, ultrasonic 30min.
Beneficial effect of the present invention is: combating vertigo granular mass control method of the present invention, provide the method differentiating Ligusticum wallichii and the red sage root in medicine, and provide the content of Puerarin composition in high effective liquid chromatography for measuring medicine, this method of quality control can control the quality of medicine better, have stronger specificity and good reappearance, real embodiment drug safety is effective, quality controllable.
Accompanying drawing explanation
Figure 1 shows that thin layer plate chromatogram differentiated by the Ligusticum wallichii of combating vertigo granular mass control method of the present invention;
Figure 2 shows that thin layer plate chromatogram differentiated by the red sage root of combating vertigo granular mass control method of the present invention;
Figure 3 shows that the puerarin content of combating vertigo granular mass control method of the present invention measures Puerarin reference substance high phase liquid chromatogram;
Figure 4 shows that the puerarin content of combating vertigo granular mass control method of the present invention measures Puerarin test sample high-efficient liquid phase chromatogram.
Embodiment
Hereafter will describe the specific embodiment of the invention in detail in conjunction with concrete accompanying drawing.It should be noted that the combination of technical characteristic or the technical characteristic described in following embodiment should not be considered to isolated, they can mutually be combined thus be reached better technique effect.
Embodiment 1, the discriminating of Ligusticum wallichii:
Get combating vertigo particle one bag, porphyrize, gets 3g, and add diethyl ether 50ml, ultrasonic 20 minutes, and filter, filtrate volatilizes, and the residue after volatilizing adds methenyl choloride 1ml, dissolved residue, as need testing solution;
Separately get Ligusticum wallichii control medicinal material 1g, add diethyl ether 20ml, is made in the same way of control medicinal material solution;
Get again not containing the negative control sample of Ligusticum wallichii, be made in the same way of negative control solution;
According to thin-layered chromatography test, draw each 5 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, with the normal hexane-ethyl acetate of volume ratio 9:1 for developping agent, launch, taking-up is dried, and inspects under putting ultraviolet lamp 365nm;
The Ligusticum wallichii of result combating vertigo granular mass as of the present invention in Fig. 1 control method differentiates that shown in thin layer plate chromatogram, in figure, 1 is Ligusticum wallichii control medicinal material, and 2-4 is test sample, and 5 is negative controls.In figure in test sample chromatogram, on the position corresponding to control medicinal material chromatogram, the fluorescence spot of aobvious same color.
Embodiment 2, the discriminating of the red sage root:
Get combating vertigo particle one bag, porphyrize, gets 3g, adds the ultrasonic 30min of 75% methyl alcohol 20ml, and filter, filtrate volatilizes, and the residue after volatilizing adds 75% methyl alcohol 1ml, and dissolved residue, as need testing solution;
Separately get protocatechualdehyde reference substance appropriate, make the reference substance solution of 1mg/ml;
Separately get red sage root control medicinal material 1g, be made in the same way of control medicinal material solution;
Get again not containing the negative control sample of the red sage root, be made in the same way of negative control solution;
According to thin-layered chromatography test, draw each 5 μ l of above-mentioned four kinds of solution, put respectively on same silica gel g thin-layer plate, take volume ratio as the toluene-methenyl choloride-acetate-methanol-formic acid of 2:3:4:0.5:2 be developping agent, launch, taking-up is dried, and inspects under putting uviol lamp 254nm;
The red sage root of result combating vertigo granular mass as of the present invention in Fig. 2 control method differentiates that shown in thin layer plate chromatogram, in figure, 1 is red sage root control medicinal material, and 2 is protocatechualdehyde reference substance, and 3-5 is test sample, and 6 is negative controls.In figure in test sample chromatogram, on the position corresponding to reference substance chromatogram, the spot of aobvious same color.
Embodiment 3, puerarin content measures,
1) determination of determined wavelength
With methyl alcohol-0.1% phosphoric acid solution (25:75) mobile phase for solvent, scan within the scope of 200-300nm to Puerarin standard items, Puerarin standard items, in this solvent, have absorption maximum at 251nm place, and light absorption value is 0.6324.
2) method of assay
Main flavour of a drug in the root of kudzu vine side of being, this law adopts the root of kudzu vine in high-efficient liquid phase technique the other side to be that index carries out assay with Puerarin.
Chromatographic condition take octadecylsilane chemically bonded silica as filling agent; With methyl alcohol-0.1% phosphoric acid solution (25:75) for mobile phase; Determined wavelength is 250nm.Number of theoretical plate calculates should be not less than 1000 by puerarin peak.
It is appropriate that Puerarin reference substance is got in the preparation of reference substance solution, accurately weighedly puts in measuring bottle, adds 30% ethanol and make the solution of every 1ml containing 80 μ g, to obtain final product.
This product that the preparation of need testing solution is got under content uniformity item is appropriate, and porphyrize, gets about 2.0g, accurately weighed, put in tool plug conical flask, precision adds 30% ethanol 50ml, close plug, weighed weight, ultrasonic process (power 160W, frequency 50kHz) 30 minutes, let cool, more weighed weight, supply the weight of less loss with 30% ethanol, shake up, get supernatant, filter, get subsequent filtrate, to obtain final product.
Assay method: accurate absorption reference substance solution and each 20 μ l of need testing solution respectively, injection liquid chromatography, measures, to obtain final product.
The puerarin content of measurement result combating vertigo granular mass as of the present invention in Fig. 3 control method measures shown in the puerarin content mensuration Puerarin test sample high-efficient liquid phase chromatogram of Puerarin reference substance high phase liquid chromatogram and Fig. 4 combating vertigo granular mass of the present invention control method.
3) system suitability
Under above-mentioned chromatographic condition, get reference substance solution, need testing solution and negative control solution each sample introduction 20 μ l respectively, record chromatogram, result: in sample, Puerarin theoretical cam curve calculates: n=2904 (> 1000); The degree of separation of main peak and impurity peaks is 1.3; Retention time is 10.9min; Show that auxiliary material and various decomposition product disturb less to main peak, meet the requirement of system suitability.
4) puerarin content measures linear relationship test
Get Puerarin reference substance 4.00mg, put in 25ml measuring bottle, add the flowing mutual-assistance and dissolve and be diluted to scale, shake up, precision measures 1,2,3,4,5ml, put in 10ml measuring bottle, add mobile phase and be diluted to scale, shake up.Precision measures each 20 μ l, respectively injection liquid chromatography, and measure peak area, carry out linear regression with peak area to sample introduction concentration (C, mg/ml), regression equation is y=42228x+543044 coefficient R
2=0.9991, result shows, Puerarin concentration is within the scope of 16 ~ 160ug/ml, and peak area and concentration linear relationship well, see the following form.
Puerarin linear relationship test findings
| Sequence number | 1 | 2 | 3 | 4 | 5 | 6 |
| Concentration (X, μ g/ml) | 16 | 32 | 48 | 64 | 80 | 160 |
| Peak area (Y) | 1156384 | 1873836 | 2681631 | 3275740 | 3873814 | 7288052 |
5) minimum detectable activity
No. 1 solution under line taking sexual intercourse test item, precision measures 2ml, put in 10ml measuring bottle, add mobile phase and be diluted to scale, shake up, precision measures 20 μ l, injection liquid chromatography respectively, measures, in chromatogram, signal to noise ratio (S/N ratio) > 10:1, so minimum detectable activity <3.2 μ g/ml.
6) precision test
Get No. 5 reference substance solution, precision measures 20 μ l, and METHOD FOR CONTINUOUS DETERMINATION 6 times, the results are shown in following table.Mean value is 3747260, RSD is 2.4%.This law precision is good.
Precision test result
| Sequence number | 1 | 2 | 3 | 4 | 5 | 6 |
| Peak area | 3873814 | 3644215 | 3826348 | 3666996 | 3756321 | 3715867 |
7) stability test
Accurate absorption need testing solution 20 μ l, sample introduction measures once at regular intervals, measures 8 hours altogether, the results are shown in following table.RSD is 2.9%, and result shows, need testing solution is basicly stable in 8 hours.
Stability test result
| Sequence number | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
| Peak area/ten thousand | 274.95 | 259.63 | 269.86 | 284.64 | 281.50 | 275.86 | 267.66 | 271.06 |
Combating vertigo granular mass control method of the present invention, provide the method differentiating Ligusticum wallichii and the red sage root in medicine, and provide the content of Puerarin composition in high effective liquid chromatography for measuring medicine, this method of quality control can control the quality of medicine better, have stronger specificity and good reappearance, real embodiment drug safety is effective, quality controllable.
Although give some embodiments of the present invention, it will be understood by those of skill in the art that without departing from the spirit of the invention herein, can change embodiment herein.Above-described embodiment is exemplary, should using embodiment herein as the restriction of interest field of the present invention.
Claims (4)
1. a method of quality control for combating vertigo particle, is characterized in that, comprises the content assaying method of Ligusticum wallichii discrimination method, red sage root discrimination method and Puerarin;
Described Ligusticum wallichii discrimination method is:
Get combating vertigo particle porphyrize, get 3g, add diethyl ether 50ml, ultrasonic 20 minutes, and filter, filtrate volatilizes, and the residue after volatilizing adds methenyl choloride 1ml, dissolved residue, as need testing solution;
Separately get Ligusticum wallichii control medicinal material 1g, add diethyl ether 20ml, is made in the same way of control medicinal material solution;
Get again not containing the negative control sample of Ligusticum wallichii, be made in the same way of negative control solution;
According to thin-layered chromatography test, draw each 5 μ l of above-mentioned three kinds of solution, put respectively on same silica gel g thin-layer plate, with the normal hexane-ethyl acetate of volume ratio 9:1 for developping agent, launch, taking-up is dried, and inspects under putting ultraviolet lamp 365nm;
Observe in test sample chromatogram, on the position corresponding to control medicinal material chromatogram, the fluorescence spot of aobvious same color.
2. the method for quality control of combating vertigo particle as claimed in claim 1, it is characterized in that, described red sage root discrimination method is:
Get combating vertigo particle porphyrize, get 3g, add the ultrasonic 30min of 75% methyl alcohol 20ml, filter, filtrate volatilizes, and the residue after volatilizing adds 75% methyl alcohol 1ml, and dissolved residue, as need testing solution;
Separately get protocatechualdehyde reference substance appropriate, make the reference substance solution of 1mg/ml;
Separately get red sage root control medicinal material 1g, be made in the same way of control medicinal material solution;
Get again not containing the negative control sample of the red sage root, be made in the same way of negative control solution;
According to thin-layered chromatography test, draw each 5 μ l of above-mentioned four kinds of solution, put respectively on same silica gel g thin-layer plate, take volume ratio as the toluene-methenyl choloride-acetate-methanol-formic acid of 2:3:4:0.5:2 be developping agent, launch, taking-up is dried, and inspects under putting uviol lamp 254nm;
Observe in test sample chromatogram, on the position corresponding to reference substance chromatogram, the spot of aobvious same color.
3. the method for quality control of combating vertigo particle as claimed in claim 1, is characterized in that, the content assaying method of described Puerarin is for adopting high effective liquid chromatography for measuring;
Chromatographic condition and system suitability take octadecylsilane chemically bonded silica as filling agent; Take volume ratio as methyl alcohol-0.1% phosphoric acid solution of 25:75 be mobile phase; High voltage and constant current wash-out, flow velocity 1.0ml/min; Determined wavelength is 250nm; Number of theoretical plate calculates >=1000 by puerarin peak;
The preparation of reference substance solution, gets Puerarin reference substance appropriate, accurately weighedly puts in measuring bottle, adds 30% ethanol and makes the solution of every 1ml containing 80 μ g, to obtain final product;
The preparation of need testing solution, gets combating vertigo particle appropriate, porphyrize, get about 2.0g, accurately weighed, put in tool plug conical flask, precision adds 30% ethanol 50ml, close plug, weighed weight, ultrasonic process, lets cool, more weighed weight, supply the weight of less loss with 30% ethanol, shake up, get supernatant, filter, get subsequent filtrate, to obtain final product;
Assay method: accurate absorption reference substance solution and each 20 μ l of need testing solution respectively, injection liquid chromatography, measures, to obtain final product.
4. the method for quality control of combating vertigo particle as claimed in claim 3, it is characterized in that, described ultrasonic process is power 160W, frequency 50kHz, ultrasonic 30min.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114166965A (en) * | 2021-11-26 | 2022-03-11 | 兰州佛慈制药股份有限公司 | Detection method of traditional Chinese medicine composition for treating vertigo |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1634261A (en) * | 2004-11-02 | 2005-07-06 | 贵阳新天药业股份有限公司 | Capsule for treating cold and cough, its preparation and detection method |
| CN1876022A (en) * | 2005-05-09 | 2006-12-13 | 贵阳云岩西创药物科技开发有限公司 | Pharmaceutical composition with red sage root and Erigeron breviscapus for treating cerebrovascular disease, its preparation process and quality control method |
| CN101672834A (en) * | 2008-12-01 | 2010-03-17 | 浙江万马药业有限公司 | Method for detecting quality of Chinese medicinal preparation for treating diabetic retinopathy |
| CN102353735A (en) * | 2011-07-13 | 2012-02-15 | 贵阳中医学院第二附属医院 | Quality detection method for Tongmai Tangyanming capsule |
| CN103940972A (en) * | 2014-04-04 | 2014-07-23 | 陕西步长高新制药有限公司 | Detection method for traditional Chinese medicine preparation for treating hypertension |
-
2015
- 2015-05-11 CN CN201510235587.9A patent/CN104807948A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1634261A (en) * | 2004-11-02 | 2005-07-06 | 贵阳新天药业股份有限公司 | Capsule for treating cold and cough, its preparation and detection method |
| CN1876022A (en) * | 2005-05-09 | 2006-12-13 | 贵阳云岩西创药物科技开发有限公司 | Pharmaceutical composition with red sage root and Erigeron breviscapus for treating cerebrovascular disease, its preparation process and quality control method |
| CN101672834A (en) * | 2008-12-01 | 2010-03-17 | 浙江万马药业有限公司 | Method for detecting quality of Chinese medicinal preparation for treating diabetic retinopathy |
| CN102353735A (en) * | 2011-07-13 | 2012-02-15 | 贵阳中医学院第二附属医院 | Quality detection method for Tongmai Tangyanming capsule |
| CN103940972A (en) * | 2014-04-04 | 2014-07-23 | 陕西步长高新制药有限公司 | Detection method for traditional Chinese medicine preparation for treating hypertension |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114166965A (en) * | 2021-11-26 | 2022-03-11 | 兰州佛慈制药股份有限公司 | Detection method of traditional Chinese medicine composition for treating vertigo |
| CN114166965B (en) * | 2021-11-26 | 2024-04-26 | 兰州佛慈制药股份有限公司 | Detection method of traditional Chinese medicine composition for treating dizziness |
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Inventor after: Wang Xidong Inventor after: Xie Guoqi Inventor after: Liu Fen Inventor after: Shang Wanbing Inventor after: Hao Shaojun Inventor after: Li Shuyi Inventor after: Li Jun Inventor after: Li Wenjun Inventor after: Fan Lili Inventor after: Zhang Zhengchen Inventor after: Sun Youshu Inventor after: Su Feng Inventor before: Wang Xidong Inventor before: Hao Shaojun Inventor before: Li Shuyi Inventor before: Li Jun Inventor before: Li Wenjun Inventor before: Fan Lili Inventor before: Zhang Zhengchen |
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