CN104805143A - Method for preparing low molecular weight gamma-polyglutamic acid - Google Patents
Method for preparing low molecular weight gamma-polyglutamic acid Download PDFInfo
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Abstract
The invention discloses a method for preparing low molecular weight gamma-polyglutamic acid. The method comprises the following steps: adjusting the pH value of a fermentation liquid to 3.5-4.0, degrading while keeping the temperature at 75-100 DEG C for 4-8 h to obtain the low molecular weight gamma-polyglutamic acid with the range of molecular weight being 10 KDa-500 KDa; adding activated carbon for decoloration and a plate frame for filtration after the temperature reduces to 0-60 DEG C, removing thallus, activated carbon and impurity protein to obtain clarified colorless feed liquid; adjusting the pH value of the feed liquid to 1.5-3.0 through hydrochloric acid, leaving the feed liquid to stand for 12-24 h, and separating out the precipitation; adding purified water to the white powder, and adjusting the pH value to 4.5-6.5, stirring the mixture for dissolution to obtain a solution with the concentration of 40% or above; then performing band drying and smashing to obtain the low molecular weight gamma-polyglutamic acid finished product. The yield of low molecular weight gamma-PGA prepared through the method reaches 85% or above and the purity reaches 95% or above, and the product quality is improved.
Description
Technical field
The present invention relates to a kind of method preparing lower molecular weight gamma-polyglutamic acid-, belong to industrial microbial technology field.
Background technology
Gamma-polyglutamic acid-(γ-PGA) is a kind of polymer aminoacid polymers by Microbe synthesis.Its environmentally safe is green bio product, has physics and chemistry and the biological characteristics of many uniquenesses such as splendid biodegradability, film-forming properties, one-tenth fibering, plasticity-, cohesiveness, moisture retention.Focus on environmental protection, emphasize today of Sustainable development; this favor being subject to people by biosynthetic degradable functional materials; just little by little be applied to medicine to manufacture; food-processing; veterinary antibiotics, sea-food antifreeze, fresh-keeping; also can Application and Development in cosmetic industry, many fields such as tobacco, leather manufacture industry and plant seed protection are a kind of Multifucntional biological products having very big Development volue and bright prospects.
The production company of current γ-PGA is fewer, and γ-PGA product is in the majority with high molecular rank.Lower molecular weight γ-PGA is that the high viscosity gamma-polyglutamic acid-fermentation liquor obtained by fermentable is crossed later stage degradation and obtained.Lower molecular weight γ-PGA can be used as novel pharmaceutical carrier, and experiment shows, the optimum weight scope that γ-PGA is used as pharmaceutical carrier is 20,000 ~ 60,000, and its molecular weight is little, and volume is little, and reactive group ratio is large, is easily combined with medicine, forms more stable mixture.Lower molecular weight γ-PGA has maintenance and strengthens the function of HA (hyaluronic acid).HA is a kind of composition of human body skin structure, keeps water profit and the elasticity of skin; Experiment proves, lower molecular weight γ-PGA efficiently can suppress the activity of Unidasa, and greatly can improve the stability of HA in face of Unidasa, thus slows down the loss of HA in skin.Lower molecular weight γ-PGA can improve natural moisturizing factor level.Natural moisturizing factor (NFM) is the hygroscopic material that skin produces, can provide and be positioned at the outermost cuticular moisture-keeping functions of skin, research report shows, at mankind TESTSKIN substratum skin, lower molecular weight γ-PGA can improve when being low to moderate the usage quantity of 0.1% in skin the level of several NMF reach 95%, skin NMF level continue to increase the water-retentivity that can promote skin.In addition, lower molecular weight γ-PGA also has enhancing epidermic cell structural integrity, improves moisture of skin and elasticity, minimizing transepidermal water loss and slow down the functions such as immune response.
Due to lower molecular weight γ-PGA complicated process of preparation; production cost is high; especially there is obstacle separation and purification and process for refining aspect for large-scale production; and numerous domestic research and development institution and enterprise lack experience in this respect; Innovation Input is inadequate; cause quality product not high, throughput is very limited, also limit applying of γ-PGA.
Summary of the invention
In the majority with high molecular rank for γ-PGA product on market, the situation that product type is less, the object of this invention is to provide a kind of method preparing lower molecular weight gamma-polyglutamic acid-, separating-purifying obtains highly purified lower molecular weight γ-PGA product, enrich γ-PGA product type, expand the application market of γ-PGA.
Technical scheme of the present invention is: a kind of method preparing lower molecular weight gamma-polyglutamic acid-, is characterized in that, comprise the following steps:
(1) fermented liquid preparation: with subtilis (Bacillus subtilis) FRD518CGMCC NO.6772 for producing bacterial strain, fermentative production gamma-polyglutamic acid-, obtains fermented liquid;
(2) high-temperature acid is degraded to obtain harmonic component gamma-polyglutamic acid-: regulate fermented liquid pH value to 3.5 ~ 4.0, temperature 75 ~ 100 DEG C, insulation degraded 4-8h, the gamma-polyglutamic acid-of more than 1000KDa in fermented liquid is degraded to the lower molecular weight gamma-polyglutamic acid-that molecular weight ranges is 10KDa-500KDa by this process; Treat that temperature is down to and be cooled to 0-60 DEG C, add activated carbon decolorizing 0-2h, after decolouring terminates, Plate Filtration, except thalline, gac and foreign protein, obtains clear, colorless feed liquid;
(3) Precipitation: feed liquid hydrochloric acid adjusts pH to 1.5-3.0, leaves standstill 12-24h, and separate out precipitation, Plate Filtration obtains white powder, and till being washed till waste water achromaticity and clarification by the purified water of pH1.5-3.0;
(4) precipitation is heavy molten: add purified water by processing through step (3) white powder obtained, and adjust pH4.5-6.5, stirring and dissolving, obtain the solution that concentration is more than 40%;
(5) belt drying: obtain lower molecular weight gamma-polyglutamic acid-finished product through belt drying, pulverizing by processing the high concentration solution obtained through step (4).
Further, the preparation method of fermented liquid in described step (1), its step is as follows:
A. seed culture
Being inoculated in by subtilis (Bacillus subtilis) FRD518CGMCC NO.6772 is equipped with in the triangular flask of seed culture medium, and in 35 ~ 37.5 DEG C, 16-32h is cultivated in the concussion of 180 ~ 220r/min shaking table, obtains seed liquor;
B. fermentation culture
Be inoculated in fermention medium by the seed liquor of step (1), inoculum size is 2 ~ 3% (mass ratioes), cultivates 45 ~ 50h, obtain fermented liquid in 35 ~ 37.5 DEG C of aeration-agitations.
Each medium component is as follows: seed culture medium: glucose 5 ~ 10g/L, yeast extract 3 ~ 5g/L, ammonium sulfate 2 ~ 10g/L, Sodium Glutamate 5 ~ 10g/L, magnesium sulfate 0.2 ~ 2g/L, pH 7.2 ~ 7.5,115 DEG C of sterilizing 20min; Fermention medium: the mixture 30 ~ 150g/L of any one or at least two kinds in sucrose, glucose, glycerine, citric acid, peptone 5 ~ 30g/L, yeast extract 5 ~ 30g/L, ammonium sulfate 5 ~ 20g/L, Sodium Glutamate 30 ~ 100g/L, magnesium sulfate 0.2 ~ 2g/L, dipotassium hydrogen phosphate 2 ~ 10g/L, pH 7.2 ~ 7.5,121 DEG C of sterilizing 20min.
Above-mentioned fermenting process also can adopt the method in the patent of invention of the applicant oneself; The application number of this patent: 201210555304.5; The applying date: 2012.11.02; Denomination of invention: gamma-polyglutamic acid-genetic engineering bacterium and high yield gamma-polyglutamic acid-method (see claim 6 and embodiment 5-6) thereof are produced in a strain.
Further, in described step (2), activated carbon dosage is 1% ~ 2% of fermented liquid weight, stirring at room temperature 60 ~ 90min.
Further, described step (5) band-type baking condition is: Heating Zone Temperature is 75 ~ 95 DEG C, and sample introduction speed is 20 ~ 30L/h, and crawler track speeds is 20 ~ 30cm/min.
The invention has the beneficial effects as follows:
(1) the high molecular γ-PGA of more than 1000KDa is degraded to the lower molecular weight γ-PGA of 10KDa-500KDa by the high temperature in the present invention, acid degradation process, has enriched γ-PGA product type, has expanded the application market of γ-PGA;
(2) the present invention utilizes the characteristic only having gamma-polyglutamic acid-Precipitation in the clear liquor of low ph value, reaches the object of separating and purifying high-purity lower molecular weight gamma-polyglutamic acid-.Acid out goes out precipitation and replaces alcohol precipitation process, not only increases product recovery rate, reduces industrial cost and danger, also save the energy;
(3) the lower molecular weight γ-PGA yield that prepared by the present invention reaches more than 85%, and purity reaches more than 95%, improves quality product.
Embodiment
Embodiment 1:
(1) fermented liquid preparation: subtilis (Bacillus subtilis) FRD518CGMCC NO.6772 is carried out fermentation culture, and obtain fermented liquid, concrete steps comprise:
A. get the bacterial classification 1 of glycerol stocks under-20 DEG C of conditions in refrigerator, inoculation articulating one ring species is in the 500ml triangular flask that 100ml seed culture medium is housed, and in 35 ~ 37.5 DEG C, 24h is cultivated in the concussion of 200r/min shaking table, and obtain seed liquor, bacterial classification concentration reaches OD
6001.5 ~ 2.0;
B. be inoculated in fermention medium by the seed liquor got ready, inoculum size is 2.5%, and cultivate 48h in 35 ~ 37.5 DEG C of aeration-agitations, obtain fermented liquid, fermentation yield can reach 67g/l.
Seed culture medium: glucose 10g/L, yeast extract 5g/L, ammonium sulfate 5g/L, Sodium Glutamate 10g/L, magnesium sulfate 1g/L, pH 7.2 ~ 7.5,115 DEG C of sterilizing 20min;
Fermention medium: sucrose 40g/L, peptone 10g/L, yeast extract 5g/L, ammonium sulfate 5g/L, Sodium Glutamate 50g/L, magnesium sulfate 1g/L, dipotassium hydrogen phosphate 10g/L, pH 7.2 ~ 7.5,121 DEG C of sterilizing 20min.
(2) harmonic component gamma-polyglutamic acid-obtains:
Regulate fermented liquid pH to 3.5, be warming up to 85 DEG C, insulation 4h, treat that temperature is down to 60 DEG C, add the activated carbon decolorizing 90min of 1%, after decolouring terminates, Plate Filtration, except thalline, gac and foreign protein, obtains clear, colorless feed liquid; The gamma-polyglutamic acid-of more than 1000KDa in fermented liquid is degraded to the lower molecular weight gamma-polyglutamic acid-that molecular weight ranges is 10KDa-500KDa by this process;
(3) Precipitation:
Above-mentioned feed liquid hydrochloric acid is adjusted pH to 1.5, leaves standstill 12h, separate out precipitation, Plate Filtration obtains white powder, and till being washed till waste water achromaticity and clarification by the purified water of pH1.5;
(4) precipitation is heavy molten:
The white powder that above-mentioned process obtains is added purified water, and adjusts pH4.5, stirring and dissolving, obtain the solution that concentration is more than 40%;
(5) belt drying:
Above-mentioned highly concentrated solution is carried out belt drying, Heating Zone Temperature is 95 DEG C, sample introduction speed is 30L/h, and crawler track speeds is 30cm/min, obtains mobility and the higher high purity and low molecular weight gamma-polyglutamic acid-(10KDa-500KDa) of solvability after crushed.
Lower molecular weight γ-PGA yield reaches 85.5%, purity 95.8%.
Embodiment 2:
(1) fermented liquid preparation: subtilis (Bacillus subtilis) FRD518CGMCC NO.6772 carries out fermentation culture, and obtain fermented liquid, concrete steps are with embodiment 1;
(2) harmonic component gamma-polyglutamic acid-obtains:
Regulate fermented liquid pH to 3.5, be warming up to 80 DEG C, insulation 6h, treat that temperature is down to about 60 DEG C, add the activated carbon decolorizing 75min of 1.5%, after decolouring terminates, Plate Filtration, except thalline, gac and foreign protein, obtains clear, colorless feed liquid; The gamma-polyglutamic acid-of more than 1000KDa in fermented liquid is degraded to the lower molecular weight gamma-polyglutamic acid-that molecular weight ranges is 10KDa-500KDa by this process;
(3) Precipitation:
Above-mentioned feed liquid hydrochloric acid is adjusted pH to 2.5, leaves standstill 24h, separate out precipitation, Plate Filtration obtains white powder, and till being washed till waste water achromaticity and clarification by the purified water of pH2.5;
(4) precipitation is heavy molten:
The white powder that above-mentioned process obtains is added purified water, and adjusts pH to 5.5, stirring and dissolving, obtain the solution that concentration is more than 40%;
(5) belt drying:
Above-mentioned highly concentrated solution is carried out belt drying, Heating Zone Temperature is 85 DEG C, sample introduction speed is 25L/h, and crawler track speeds is 25cm/min, obtains mobility and the higher high purity and low molecular weight gamma-polyglutamic acid-(10KDa-500KDa) of solvability after crushed.
Lower molecular weight γ-PGA yield reaches 86.1%, purity 95.8%.
Embodiment 3:
(1) fermented liquid preparation: subtilis (Bacillus subtilis) FRD518CGMCC NO.6772 carries out fermentation culture, and obtain fermented liquid, concrete steps are with embodiment 1;
(2) harmonic component gamma-polyglutamic acid-obtains:
Regulate fermented liquid pH to 4.0, be warming up to 85 DEG C, insulation 5h, treat that temperature is down to 60 DEG C, add the activated carbon decolorizing 60min of 2%, after decolouring terminates, Plate Filtration, except thalline, gac and foreign protein, obtains clear, colorless feed liquid; The gamma-polyglutamic acid-of more than 1000KDa in fermented liquid is degraded to the lower molecular weight gamma-polyglutamic acid-that molecular weight ranges is 10KDa-500KDa by this process;
(3) Precipitation:
Above-mentioned feed liquid hydrochloric acid is adjusted pH to 3.0, leaves standstill 24h, separate out precipitation, Plate Filtration obtains white powder, and till being washed till waste water achromaticity and clarification by the purified water of pH3.0;
(4) precipitation is heavy molten:
The white powder that above-mentioned process obtains is added purified water, and adjusts pH to 6.5, stirring and dissolving, obtain the solution that concentration is more than 40%;
(5) belt drying:
Above-mentioned highly concentrated solution is carried out belt drying, Heating Zone Temperature is 75 DEG C, sample introduction speed is 20L/h, and crawler track speeds is 20cm/min, obtains mobility and the higher high purity and low molecular weight gamma-polyglutamic acid-(10KDa-500KDa) of solvability after crushed.
Lower molecular weight γ-PGA yield reaches 85.5%, purity 95.8%.
Claims (6)
1. prepare a method for lower molecular weight gamma-polyglutamic acid-, it is characterized in that, comprise the following steps:
(1) fermented liquid preparation: with subtilis (Bacillus subtilis) FRD518CGMCC NO.6772 for producing bacterial strain, fermentative production gamma-polyglutamic acid-, obtains fermented liquid;
(2) high-temperature acid is degraded to obtain harmonic component gamma-polyglutamic acid-: regulate fermented liquid pH value to 3.5 ~ 4.0, temperature 75 ~ 100 DEG C, and insulation degraded 4-8h, obtains the lower molecular weight gamma-polyglutamic acid-that molecular weight ranges is 10KDa-500KDa; Treat that temperature is down to and be cooled to 0-60 DEG C, add activated carbon decolorizing 0-2h, after decolouring terminates, Plate Filtration, except thalline, gac and foreign protein, obtains clear, colorless feed liquid;
(3) Precipitation: feed liquid hydrochloric acid adjusts pH to 1.5-3.0, leaves standstill 12-24h, and separate out precipitation, Plate Filtration obtains white powder, and till being washed till waste water achromaticity and clarification by the purified water of pH1.5-3.0;
(4) precipitation is heavy molten: add purified water by processing through step (3) white powder obtained, and adjust pH4.5-6.5, stirring and dissolving, obtain the solution that concentration is more than 40%;
(5) belt drying: obtain lower molecular weight gamma-polyglutamic acid-finished product through belt drying, pulverizing by processing the high concentration solution obtained through step (4).
2. a kind of method preparing lower molecular weight gamma-polyglutamic acid-as claimed in claim 1, is characterized in that,
A. seed culture
Being inoculated in by subtilis (Bacillus subtilis) FRD518CGMCC NO.6772 is equipped with in the triangular flask of seed culture medium, and in 35 ~ 37.5 DEG C, 16-32h is cultivated in the concussion of 180 ~ 220r/min shaking table, obtains seed liquor;
B. fermentation culture
Be inoculated in fermention medium by the seed liquor of step (1), inoculum size is 2 ~ 3%, cultivates 45 ~ 50h, obtain fermented liquid in 35 ~ 37.5 DEG C of aeration-agitations.
3. a kind of method preparing lower molecular weight gamma-polyglutamic acid-as claimed in claim 2, it is characterized in that, the component of described seed culture medium and content are: glucose 5 ~ 10g/L, yeast extract 3 ~ 5g/L, ammonium sulfate 2 ~ 10g/L, Sodium Glutamate 5 ~ 10g/L, magnesium sulfate 0.2 ~ 2g/L, pH 7.2 ~ 7.5,115 DEG C of sterilizing 20min.
4. a kind of method preparing lower molecular weight gamma-polyglutamic acid-as claimed in claim 2, it is characterized in that, the component of described fermention medium and content are: the mixture 30 ~ 150g/L of any one or at least two kinds in sucrose, glucose, glycerine, citric acid, peptone 5 ~ 30g/L, yeast extract 5 ~ 30g/L, ammonium sulfate 5 ~ 20g/L, Sodium Glutamate 30 ~ 100g/L, magnesium sulfate 0.2 ~ 2g/L, dipotassium hydrogen phosphate 2 ~ 10g/L, pH 7.2 ~ 7.5,121 DEG C of sterilizing 20min.
5. as a kind of method preparing lower molecular weight gamma-polyglutamic acid-in claim 1-4 as described in any one, it is characterized in that, in described step (2), activated carbon dosage is 1% ~ 2% of fermented liquid weight, stirring at room temperature 60 ~ 90min.
6. as a kind of method preparing lower molecular weight gamma-polyglutamic acid-in claim 1-4 as described in any one, it is characterized in that, described step (5) band-type baking condition is: Heating Zone Temperature is 75 ~ 95 DEG C, and sample introduction speed is 20 ~ 30L/h, and crawler track speeds is 20 ~ 30cm/min.
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Cited By (5)
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| CN105968383A (en) * | 2016-04-05 | 2016-09-28 | 山东省药学科学院 | Low-molecular-weight polyglutamic acid, and preparation method and application thereof |
| CN110372858A (en) * | 2019-08-30 | 2019-10-25 | 尚科生物医药（上海）有限公司 | A kind of method of polyglutamic acid extraction and purification |
| CN110498919A (en) * | 2019-07-26 | 2019-11-26 | 河南师范大学 | A kind of synthetic method for the γ-PGA-Bn that esterification degree is controllable |
| CN114774488A (en) * | 2022-05-13 | 2022-07-22 | 山东福瑞达生物科技有限公司 | Production method of low-endotoxin gamma-polyglutamic acid |
| CN117720722A (en) * | 2023-12-19 | 2024-03-19 | 山东福瑞达生物科技有限公司 | Preparation method and application of oligoglutamic acid |
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Cited By (5)
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| CN114774488A (en) * | 2022-05-13 | 2022-07-22 | 山东福瑞达生物科技有限公司 | Production method of low-endotoxin gamma-polyglutamic acid |
| CN117720722A (en) * | 2023-12-19 | 2024-03-19 | 山东福瑞达生物科技有限公司 | Preparation method and application of oligoglutamic acid |
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