CN104800825A - Method for inducing osteoblast differentiation through MAPK and NF-kB by utilizing peptide separated from microalgae - Google Patents
Method for inducing osteoblast differentiation through MAPK and NF-kB by utilizing peptide separated from microalgae Download PDFInfo
- Publication number
- CN104800825A CN104800825A CN201510183992.0A CN201510183992A CN104800825A CN 104800825 A CN104800825 A CN 104800825A CN 201510183992 A CN201510183992 A CN 201510183992A CN 104800825 A CN104800825 A CN 104800825A
- Authority
- CN
- China
- Prior art keywords
- peptide
- microalgae
- supernatant
- osteoblast differentiation
- mapk
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a method for inducing osteoblast differentiation through MAPK and NF-kB by utilizing peptide separated from microalgae. The method comprises the following steps: taking a fresh animal limb bone; carrying out cleaning, disinfecting and smashing; adding distilled water into the bone powder; carrying out hydrolysis in a thermostatic reaction tank under the control of protease; carrying out centrifugation to obtain a supernatant I; degreasing the supernatant I; concentrating the degreased supernatant; carrying out ethanol extraction; filtering out a supernatant II; dealcoholizing the supernatant II; carrying out centrifugal ultrafiltration on the dealcoholized supernatant; collecting a filtered solution to obtain bioactive bone peptide; preparing the bioactive bone peptide into any pharmacopedic dosage form. The method has the advantages that the technology conditions are mild, and all lower than 60 DEG C; the biological activity of micro-molecule polypeptide can be maintained easily; the obtained bioactive bone peptide is smaller than or equal to 10,000 dalton in molecular weight, is certain in enhancing and differentiation effects on osteoblast, high in biological activity, and easy to be absorbed and utilized by a human body, can be made into an intestinal absorption preparation easily, is remarkable in bioavailability improvement, and can be made into health food.
Description
Technical field
The invention belongs to biological technical field, particularly relate to a kind of peptide separated from microalgae by MAPK and NF-kB inducing osteoblast differentiation method.
Background technology
Osteoporosis (osteoporosis) is that a kind of Calx of osseous tissue reduces and makes that bone compact substance is thinning, medullary cavity becomes large disease, along with symptom progress, bone is died down, also easily fractures even if be subject to less impact.
Determine that the bone amount of bone health state can be subject to the impact of the various factors such as difference of heredity, nutrition intake, Hormone change, motion and living habit, especially old-age group, hypomotility, under-weight, smoking, love are eaten low calcium food, menolipsis or are cut off the reason that ovary etc. is considered to osteoporosis.
Summary of the invention
The object of the embodiment of the present invention is to provide a kind of peptide separated from microalgae by MAPK and NF-kB inducing osteoblast differentiation method, be intended to the domestic understanding for osteoporosis of solution comparatively not enough and cannot properly carry out preventing and treat, the secondary osteoporosis wherein caused owing to abusing medicine presents the problem of serious trend gradually.
The present invention is achieved in that a kind of peptide separated from microalgae is by MAPK and NF-kB inducing osteoblast differentiation method, and the described peptide separated from microalgae is comprised by MAPK and NF-kB inducing osteoblast differentiation method:
Get fresh animal foot bone, cleaning, sterilization, pulverize, add distilled water, with protease controlled hydrolysis in isothermal reaction tank, get supernatant for centrifugal point;
Supernatant defat, concentrated, ethanol extraction, leaches supernatant;
Supernatant dealcoholysis, centrifugal ultrafiltration, collects filtered solution and obtains biological activity bone peptide;
Dosage form described on any one pharmaceutics made by active bone peptide raw material.
Further, the peptide composition separated in microalgae:
Gly-Gln-Gly-Phe-Ser-Tyr-Pro-Tyr-Lys-Ala-Val-Phe-Ser-Thr-Gln。
Further, pharmaceutical composition comprises osteoblast differentiation or the bone formation promotion synthetic peptide of the peptide separated from microalgae.
Further, described osteoblast differentiation or bone formation promotion synthetic peptide are used as effective ingredient and treat osteoporosis, osteoarthritis or Cranial defect.
Further, the effective dose of described osteoblast differentiation or bone formation promotion synthetic peptide is 0.1 to 2 μ g/ml.
The synthetic peptide that the present invention contains BFP 1 can be promote osteoblastic differentiation in osteoblast differentiation, is therefore applicable to the various fields needing to promote osteoblast differentiation.The effective ingredient of pharmaceutical composition of the present invention comprises the synthetic peptide containing BFP1, when treating osteoporosis and/or osteoarthritis, its drug effect is excellent and be free from side effects, production cost is more relatively low than existing therapeutant, and the excellent curative effect being representative with osteoblast differentiation facilitation effect is enough to alternative existing therapeutant.And the effective ingredient of pharmaceutical composition of the present invention comprises the synthetic peptide containing BFP1, its drug effect is excellent and be free from side effects, and production cost is relatively low and Bone Defect Repari effect is remarkable.
Accompanying drawing explanation
Fig. 1 is the flow chart of the peptide separated from microalgae that provides of the embodiment of the present invention by MAPK and NF-kB inducing osteoblast differentiation method.
Detailed description of the invention
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Below in conjunction with drawings and the specific embodiments, application principle of the present invention is further described.
As shown in Figure 1, the embodiment of the present invention is achieved in that a kind of peptide separated from microalgae is comprised by MAPK and NF-kB inducing osteoblast differentiation method:
S101: get fresh animal foot bone, cleaning, sterilization, pulverizes, adds distilled water, with protease controlled hydrolysis in isothermal reaction tank, get supernatant for centrifugal point;
S102: supernatant defat, concentrated, ethanol extraction, leaches supernatant;
S103: supernatant dealcoholysis, centrifugal ultrafiltration, collects filtered solution and obtains biological activity bone peptide;
S104: dosage form described on any one pharmaceutics made by active bone peptide raw material.
The peptide composition separated in microalgae:
Gly-Gln-Gly-Phe-Ser-Tyr-Pro-Tyr-Lys-Ala-Val-Phe-Ser-Thr-Gln。
Pharmaceutical composition comprises osteoblast differentiation or the bone formation promotion synthetic peptide of the peptide separated from microalgae.
Described osteoblast differentiation or bone formation promotion synthetic peptide are used as effective ingredient and treat osteoporosis, osteoarthritis or Cranial defect.
The effective dose of described osteoblast differentiation or bone formation promotion synthetic peptide is 0.1 to 2 μ g/ml.
A kind of peptide of osteoblast differentiation, EPQWFL (MW=908.9 dalton), it is by the tunning that obtain of multiple-shaped nuohan inferior yeast from Microalgae fermentation, result shows, the peptide separated from fermentation bar husband algae can increase the activity of alkali phosphatase (ALP) as early stage osteoblast differentiation phenotypic markers, in order to illustrate the mechanism of action of the peptide separated in described fermentation bar husband algae, the impact of described peptide on the activation of MAPK/NF-κ B is studied, determine that PFPL process significantly can cause the rising of MAP (P-P38) kinases/NF-κ B (P60) in MG-63 cell.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (5)
1. the peptide separated from microalgae, by a MAPK and NF-kB inducing osteoblast differentiation method, is characterized in that, the described peptide separated from microalgae is comprised by MAPK and NF-kB inducing osteoblast differentiation method:
Get fresh animal foot bone, cleaning, sterilization, pulverize, add distilled water, with protease controlled hydrolysis in isothermal reaction tank, get supernatant for centrifugal point;
Supernatant defat, concentrated, ethanol extraction, leaches supernatant;
Supernatant dealcoholysis, centrifugal ultrafiltration, collects filtered solution and obtains biological activity bone peptide;
Dosage form described on any one pharmaceutics made by active bone peptide raw material.
2. the peptide separated from microalgae as claimed in claim 1, by MAPK and NF-kB inducing osteoblast differentiation method, is characterized in that, the peptide composition separated in microalgae:
Gly-Gln-Gly-Phe-Ser-Tyr-Pro-Tyr-Lys-Ala-Val-Phe-Ser-Thr-Gln。
3. the peptide separated from microalgae is as claimed in claim 1 by MAPK and NF-kB inducing osteoblast differentiation method, and it is characterized in that, pharmaceutical composition comprises osteoblast differentiation or the bone formation promotion synthetic peptide of the peptide separated from microalgae.
4. the peptide separated from microalgae is as claimed in claim 3 by MAPK and NF-kB inducing osteoblast differentiation method, it is characterized in that, described osteoblast differentiation or bone formation promotion synthetic peptide are used as effective ingredient and treat osteoporosis, osteoarthritis or Cranial defect.
5. the peptide separated from microalgae is as claimed in claim 3 by MAPK and NF-kB inducing osteoblast differentiation method, and it is characterized in that, the effective dose of described osteoblast differentiation or bone formation promotion synthetic peptide is 0.1 to 2 μ g/ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510183992.0A CN104800825A (en) | 2015-04-20 | 2015-04-20 | Method for inducing osteoblast differentiation through MAPK and NF-kB by utilizing peptide separated from microalgae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510183992.0A CN104800825A (en) | 2015-04-20 | 2015-04-20 | Method for inducing osteoblast differentiation through MAPK and NF-kB by utilizing peptide separated from microalgae |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104800825A true CN104800825A (en) | 2015-07-29 |
Family
ID=53686304
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510183992.0A Pending CN104800825A (en) | 2015-04-20 | 2015-04-20 | Method for inducing osteoblast differentiation through MAPK and NF-kB by utilizing peptide separated from microalgae |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104800825A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113698451A (en) * | 2021-08-02 | 2021-11-26 | 广东海洋大学 | Microalgae polypeptide and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1843502A (en) * | 2006-01-16 | 2006-10-11 | 广东医学院 | Active bone peptide for treating and preventing osteoporosis and its preparing method |
| CN101868245A (en) * | 2008-05-16 | 2010-10-20 | 全南大学校产学协力团 | An synthetic peptide containing bone forming peptide 1(bfp 1) for stimulating osteoblast differentiation, and pharmaceutical composition comprising the synthetic peptide |
| CN103667182A (en) * | 2012-12-17 | 2014-03-26 | 湖州市中心医院 | Inducing method and inducing culture medium for differentiation of bone marrow mesenchymal stem cells into osteoblasts in vitro |
-
2015
- 2015-04-20 CN CN201510183992.0A patent/CN104800825A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1843502A (en) * | 2006-01-16 | 2006-10-11 | 广东医学院 | Active bone peptide for treating and preventing osteoporosis and its preparing method |
| CN101868245A (en) * | 2008-05-16 | 2010-10-20 | 全南大学校产学协力团 | An synthetic peptide containing bone forming peptide 1(bfp 1) for stimulating osteoblast differentiation, and pharmaceutical composition comprising the synthetic peptide |
| CN103667182A (en) * | 2012-12-17 | 2014-03-26 | 湖州市中心医院 | Inducing method and inducing culture medium for differentiation of bone marrow mesenchymal stem cells into osteoblasts in vitro |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113698451A (en) * | 2021-08-02 | 2021-11-26 | 广东海洋大学 | Microalgae polypeptide and preparation method and application thereof |
| CN113698451B (en) * | 2021-08-02 | 2023-04-11 | 广东海洋大学 | Microalgae polypeptide and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20120040908A1 (en) | Fracture repair promoter | |
| CN109364194A (en) | It maintains bone health and treats the composition of the osteoarthropathy in osteoarthritis and joint | |
| JP5213332B2 (en) | Egg-derived bone strengthening composition | |
| CN101297853B (en) | Bone-melon extract injection and preparation thereof | |
| CN102188692A (en) | Bone peptide composition, preparation thereof, preparation method thereof and application | |
| CN104800825A (en) | Method for inducing osteoblast differentiation through MAPK and NF-kB by utilizing peptide separated from microalgae | |
| CN105124583A (en) | Health-care food capable of enhancing bone mineral density and preparation method thereof | |
| CN112322681B (en) | Traditional Chinese medicine maggot active peptide and preparation method and application thereof | |
| CN115969961A (en) | Calcium, VD and protein peptide composition for improving bone mineral density and preparation method and application thereof | |
| CN109320600B (en) | Novel PTH mimic peptide based on protein domain reconstruction and application thereof | |
| JP2014125482A (en) | Function improving agent for tendon and ligament, pharmaceutical composition, food and feedstuff containing the same | |
| JPWO2009057282A1 (en) | Food material for suppressing bone resorption | |
| CN106366176B (en) | PTH mimetic peptides and uses thereof | |
| Vodoslavskyi | The quantitative content of the phenolic compounds in the Stellaria media herb | |
| CN112245452A (en) | Preparation method of bone extract and application of bone extract to osteoporosis and osteoarthritis | |
| KR20160127390A (en) | Health functional composition extracted from antler for preventing or treating the osteoporosis | |
| JP6304731B2 (en) | Anti-influenza virus composition containing Karachiachi extract as active ingredient | |
| TWI574696B (en) | Novel protein material | |
| CN101371714A (en) | Plateau yak placenta element health product and preparation method thereof | |
| Vujasinović-Stupar et al. | Supplementation with bio-calcium from shells Pinctada maxima in postmenopausal women with decreased mineral bone density: Pilot study | |
| CN105520135A (en) | Healthy food with function of treating osteoporosis | |
| CA2770797C (en) | Composite and method of use for facilitating bone ossification | |
| CN101390880A (en) | Use of sea collagen peptides in preparing medicine or food for protecting kidney function, delaying chronic renal failure | |
| RU2589263C1 (en) | Use of male bee brood with calcium compounds for accelerating consolidation of bone fractures | |
| CN105111150A (en) | Medicine composition for treating osteoporosis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150729 |
|
| WD01 | Invention patent application deemed withdrawn after publication |