CN104782584B - A kind of mango pollination fly draws fly matrix and its application - Google Patents
A kind of mango pollination fly draws fly matrix and its application Download PDFInfo
- Publication number
- CN104782584B CN104782584B CN201510229482.2A CN201510229482A CN104782584B CN 104782584 B CN104782584 B CN 104782584B CN 201510229482 A CN201510229482 A CN 201510229482A CN 104782584 B CN104782584 B CN 104782584B
- Authority
- CN
- China
- Prior art keywords
- fly
- mango
- matrix
- pollination
- fruit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 235000004936 Bromus mango Nutrition 0.000 title claims abstract description 62
- 235000014826 Mangifera indica Nutrition 0.000 title claims abstract description 62
- 235000009184 Spondias indica Nutrition 0.000 title claims abstract description 62
- 239000011159 matrix material Substances 0.000 title claims abstract description 51
- 230000010152 pollination Effects 0.000 title claims abstract description 46
- 240000007228 Mangifera indica Species 0.000 title claims 2
- 241001093152 Mangifera Species 0.000 claims abstract description 60
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 24
- 235000015097 nutrients Nutrition 0.000 claims abstract description 8
- ZYEMGPIYFIJGTP-UHFFFAOYSA-N O-methyleugenol Chemical group COC1=CC=C(CC=C)C=C1OC ZYEMGPIYFIJGTP-UHFFFAOYSA-N 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 8
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 6
- 150000002475 indoles Chemical class 0.000 claims description 5
- 235000015099 wheat brans Nutrition 0.000 claims description 4
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical group [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 3
- 240000007594 Oryza sativa Species 0.000 claims description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims description 3
- 235000009754 Vitis X bourquina Nutrition 0.000 claims description 3
- 235000012333 Vitis X labruscana Nutrition 0.000 claims description 3
- 240000006365 Vitis vinifera Species 0.000 claims description 3
- 235000014787 Vitis vinifera Nutrition 0.000 claims description 3
- 239000000908 ammonium hydroxide Substances 0.000 claims description 3
- 235000009566 rice Nutrition 0.000 claims description 3
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 3
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 2
- 229920002678 cellulose Polymers 0.000 claims description 2
- 239000001913 cellulose Substances 0.000 claims description 2
- 239000003153 chemical reaction reagent Substances 0.000 claims description 2
- 235000019698 starch Nutrition 0.000 claims description 2
- 239000008107 starch Substances 0.000 claims description 2
- 241000202815 Chrysomya megacephala Species 0.000 abstract description 7
- 241000196324 Embryophyta Species 0.000 abstract description 5
- 241000257159 Musca domestica Species 0.000 abstract description 4
- 238000012545 processing Methods 0.000 description 17
- 230000000694 effects Effects 0.000 description 3
- 241000255925 Diptera Species 0.000 description 2
- 206010000210 abortion Diseases 0.000 description 2
- 231100000176 abortion Toxicity 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- IGOWHGRNPLFNDJ-ZPHPHTNESA-N (z)-9-tricosene Chemical compound CCCCCCCCCCCCC\C=C/CCCCCCCC IGOWHGRNPLFNDJ-ZPHPHTNESA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 1
- 229910000013 Ammonium bicarbonate Inorganic materials 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 240000008790 Musa x paradisiaca Species 0.000 description 1
- 235000018290 Musa x paradisiaca Nutrition 0.000 description 1
- 235000012538 ammonium bicarbonate Nutrition 0.000 description 1
- 239000001099 ammonium carbonate Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethanethiol Chemical compound CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000005078 fruit development Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000002420 orchard Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention relates to mango cultivation field, discloses a kind of mango pollination fly and draw fly matrix and its application.The component that fly matrix includes following mass fraction that draws drives 0.1-1 parts of taste agent, 0.5-1.5 parts of fruit, 3-7 parts of nutrient matrix；It is 7-8 that the mango pollination fly, which draws fly matrix pH value,.10-15d will draw fly device and be placed in Mango Fruit Garden before mango full blossom.It is mango flower pollination that chrysomyia megacephala and housefly, which can be attracted, draws that fly type is relatively single-minded, and tree amount obviously increases on the fly that pollinates, and effectively improves No. 1 mango fruit-setting rate of platform agriculture, normal fruit ratio, mean fruit weight and average single plant yield.
Description
Technical field
The present invention relates to mango cultivation field, particularly relates to a kind of mango pollination fly and draw fly matrix and its application.
Background technique
In recent years, mango commercial cultivars are numerous, such as platform agriculture 1, the bright awns of gold.In recent years, in mango cultivation
Fruitlet rate, abortion fruit ratio sharply increase, and have seriously affected the quality and commercial value of mango.Bad Pollination Fertilization is to lead to awns
One of the main reason for fruit fruitlet or abortion fruit.Fly is the main pollination medium of mango, is with chrysomyia megacephala again in the fly that pollinates
Main pollination flies.It hangs in orchard, draws currently, being generally used as using dead fish, slough etc. in mango cultivation and drawing fly bait
Pollination fly is lured to pollinate to mango, to improve Pollination Fertilization rate.But since the bait type of use is more, there is attracted mostly
The not single-minded and used bait source of the fly type come is restricted, and quantity is difficult to ensure, and easy to be corrupt, rotten,
It gives out a foul smell, breed germ, generate various problems.
Summary of the invention
The invention proposes a kind of mango pollination flies to draw fly matrix, can attract chrysomyia megacephala and housefly, effectively improve platform
The fruit-setting rate of No. 1 mango of agriculture.
The present invention also provides the application methods for drawing fly matrix.
The technical scheme of the present invention is realized as follows:
A kind of mango pollination fly draws fly matrix, which is characterized in that the component including following mass fraction: drives taste agent 0.1-1
Part, 0.5-1.5 parts of fruit, 3-7 parts of nutrient matrix；It is 7-8 that the mango pollination fly, which draws fly matrix pH value,；The drive taste agent
For eugenol methyl ether or indoles with indoles smell, the fruit is grape or apple, the nutrient matrix mainly at
It is divided into starch and cellulose.
Further, the nutrient matrix is wheat bran or rice bran.
Further, adjusting reagent used in pH value is ammonium hydroxide or sodium bicarbonate.
A kind of application of mango pollination fly matrix, 10-15d is placed in Mango Fruit Garden before mango full blossom.
Beneficial effects of the present invention:
Mango pollination fly of the present invention draws fly matrix, and drive taste agent therein can attract chrysomyia megacephala and housefly,
In based on chrysomyia megacephala, it is relatively single-minded to draw fly type；Sugar rich in fruit has unique fragrant and sweet gas
Taste is used with taste agent is driven with contract, and common play draws fly effect；Nutrient matrix is not only that fly blow provides sufficient nutrition, is also had
Loose porous structure breathes freely good, is conducive to the hatching and larvae development of fly blow；Entire matrix is in alkalescent, be fly blow and
Larva provides suitable living environment, improves the survival rate of larva, and then improve the upper tree amount of pollination fly.It is of the present invention
Mango pollination fly draws fly matrix, and each component is deployed mutually, is played a role jointly, indispensable.Count fruit-setting rate and fruit growth
Developmental state, by Mango Fruit Garden place it is of the present invention draw fly matrix, can effectively improve mango fruit-setting rate, normal fruit ratio
Rate, mean fruit weight and evaluation single plant yield.Mango pollination fly of the present invention draws fly matrix, and matrix used is from a wealth of sources,
The long period can be saved, corruption is not easy, breeds bacterium, cleaning, health.
Specific embodiment
Below in conjunction with the embodiment of the present invention, the technical solution in the present invention is clearly and completely described, it is clear that
Described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on the implementation in the present invention
Example, every other embodiment obtained by those of ordinary skill in the art without making creative efforts belong to
The scope of protection of the invention.
Embodiment 1
A kind of mango pollination fly draws fly matrix, the component including following mass fraction: eugenol methyl ether 0.1g, grape
0.5g, wheat bran 3g add ammonium hydroxide tune pH value to 7.
A kind of application of mango pollination fly matrix, the mango pollination fly draw fly matrix 10d before mango full blossom and are placed on
It supports in fly device, and is placed in Mango Fruit Garden.
Embodiment 2
A kind of mango pollination fly draws fly matrix, the component including following mass fraction: indoles 0.5g, banana 1g, wheat bran 2g,
Add sodium bicarbonate tune pH value to 7.5.
A kind of application of mango pollination fly matrix, the mango pollination fly draw fly matrix 15d before mango full blossom and are placed on
It supports in fly device, and is placed in Mango Fruit Garden.
Embodiment 3
A kind of mango pollination fly draws fly matrix, the component including following mass fraction: eugenol methyl ether 1g, apple 1.5g,
Rice bran 3g adds ammonium hydrogen carbonate tune pH value to 8.
A kind of application of mango pollination fly matrix, after above-mentioned each component is mixed by formula ratio, 10d is placed before mango full blossom
In feeding fly device, and it is placed in Mango Fruit Garden.
Fly matrix of the present invention of drawing draws fly amount and pollination situation measurement
Mango similar in growing way, the age of tree is selected, is averagely allocated as 9 groups, experimental group is prepared in the following way respectively draws
Fly matrix, 15d before full blossom, the fly matrix of drawing of equivalent is placed in feeding fly device, and is placed in No. 1 Mango Fruit Garden of platform agriculture, right
It is without any processing according to organizing.Tree amount on pollination fly is counted after 10d, investigates its fruit-setting rate after second of physiological fallen fruit, tune when mature
Look into normal fruit rate, mean fruit weight and average single plant yield.Its result such as table 1.
Processing group 1: mango pollination fly is prepared according to the proportion that embodiment 1 provides and draws fly matrix；
Processing group 2: mango pollination fly is prepared according to the proportion that embodiment 2 provides and draws fly matrix；
Processing group 3: mango pollination fly is prepared according to the proportion that embodiment 3 provides and draws fly matrix；
Processing group 4: removing in component and drive taste agent, remaining component is same as Example 1；
Processing group 5: removing fruit in component, remaining component is same as Example 1；
Processing group 6: removing nutrient matrix in component, remaining component is same as Example 1:
Processing group 7: component proportion is same as Example 1, but the pH value of matrix is adjusted to 6；
Processing group 8: component proportion is same as Example 1, but the pH value of matrix is adjusted to 9.
1 difference of table draws fly matrix and draws fly amount and pollination situation
The data of table 1 are analyzed, from the point of view of drawing fly type, in addition to processing group 4, other processing groups draw the type of fly kind
Predominantly chrysomyia megacephala, housefly, based on chrysomyia megacephala, and processing group 4 eliminates and drives taste agent, other than above two fly,
Various other non-pollination flies are also attracted, it is mixed and disorderly to draw fly type.Illustrate that mango pollination fly of the present invention draws fly matrix to awns
Fruit pollination fly has attracted larger, and it is single-minded to draw fly type.
It sets and takes temperature from larva quantity and pollination fly, tree amount is above it in 3 embodiment group larva quantity and pollination fly
His processing group, compared with the control group, difference is more significant.Although 4 larva number of processing group is not much different with 3 embodiment groups,
It is due to drawing fly wide variety, tree amount is still significantly less than 3 embodiment groups on the fly that pollinates.Illustrate mango of the present invention
Pollination fly, which draws fly matrix, can significantly improve tree amount on mango pollination fly.
From bear fruit with from the point of view of fruit development, 3 embodiment groups, fruit-setting rate, normal fruit ratio, mean fruit weight and
Average single plant yield, is not only apparently higher than control group, also there were significant differences compared with other processing groups.
In summary experimental data, 3 embodiment groups single-minded attraction mango can not only pollinate fly, but also the fly that pollinates relatively
Upper tree amount, fruit-setting rate, normal fruit rate, single fruit weight and average single plant yield are superior to control group, illustrate mango of the present invention
Pollination fly, which draws fly matrix, can significantly improve mango pollination rate, improve Mango Output and marketing quality.Meanwhile with processing group 4 to
Processing group 8 compares, and 3 embodiment groups illustrate that mango pollination fly of the present invention draws fly matrix, respectively there is also significant difference
Component effect is mutually promoted, and is played a role jointly, and lacking any of them one will affect the performance for entirely drawing fly matrix effect.
In addition, of the present invention draw fly matrix, the shelf-life is longer, can play a role in a long time；Original used
Expect from a wealth of sources, can satisfy that Mango Fruit Garden is a wide range of, demand of duration, cleaning, health will not give out strong stench
Taste.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (2)
1. a kind of mango pollination fly draws fly matrix, which is characterized in that the component including following mass fraction: driving 0.1-1 parts of taste agent,
0.5-1.5 parts of fruit, 3-7 parts of nutrient matrix；It is 7-8 that the mango pollination fly, which draws fly matrix pH value,；The drive taste agent is
Eugenol methyl ether or indoles with indoles smell, the fruit be grape or apple, the nutrient matrix be wheat bran or
Rice bran, main component are starch and cellulose；Adjusting reagent used in pH value is ammonium hydroxide or sodium bicarbonate.
2. a kind of application of mango pollination fly matrix according to claim 1, it is characterised in that: the 10- before mango full blossom
15d is placed in Mango Fruit Garden.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510229482.2A CN104782584B (en) | 2015-05-07 | 2015-05-07 | A kind of mango pollination fly draws fly matrix and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510229482.2A CN104782584B (en) | 2015-05-07 | 2015-05-07 | A kind of mango pollination fly draws fly matrix and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104782584A CN104782584A (en) | 2015-07-22 |
| CN104782584B true CN104782584B (en) | 2019-05-10 |
Family
ID=53548144
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510229482.2A Expired - Fee Related CN104782584B (en) | 2015-05-07 | 2015-05-07 | A kind of mango pollination fly draws fly matrix and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104782584B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113598044B (en) * | 2021-08-24 | 2022-04-22 | 华中农业大学 | Method for pollinating early spring crops by domesticated insects at low temperature |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1555706A (en) * | 2004-01-01 | 2004-12-22 | 胡锡林 | Fly trapping agent and its manufacturing method and fly trapping method and fly catcher |
| RU2253987C2 (en) * | 2002-11-18 | 2005-06-20 | Всероссийский научно-исследовательский институт консервной и овощесушильной промышленности (Государственное научное учреждение) | Method for manufacturing acaricide-insecticide composition |
| CN101268786A (en) * | 2008-04-18 | 2008-09-24 | 浙江大学 | Winged insect attractant |
| CN102396538A (en) * | 2010-09-09 | 2012-04-04 | 蔡冬祎 | Fly eradication attractant |
| CN102599049A (en) * | 2012-03-28 | 2012-07-25 | 何寒 | Pollination method of mango by breeding flies in flowering phase for propagation |
| CN103270943A (en) * | 2013-05-20 | 2013-09-04 | 杨健 | Attractant capable of promoting cross pollination of fruit trees and improving fruit set percentage of fruit trees |
| CN104430584A (en) * | 2014-12-03 | 2015-03-25 | 红河学院 | Guava fruit fly attractant |
-
2015
- 2015-05-07 CN CN201510229482.2A patent/CN104782584B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2253987C2 (en) * | 2002-11-18 | 2005-06-20 | Всероссийский научно-исследовательский институт консервной и овощесушильной промышленности (Государственное научное учреждение) | Method for manufacturing acaricide-insecticide composition |
| CN1555706A (en) * | 2004-01-01 | 2004-12-22 | 胡锡林 | Fly trapping agent and its manufacturing method and fly trapping method and fly catcher |
| CN101268786A (en) * | 2008-04-18 | 2008-09-24 | 浙江大学 | Winged insect attractant |
| CN102396538A (en) * | 2010-09-09 | 2012-04-04 | 蔡冬祎 | Fly eradication attractant |
| CN102599049A (en) * | 2012-03-28 | 2012-07-25 | 何寒 | Pollination method of mango by breeding flies in flowering phase for propagation |
| CN103270943A (en) * | 2013-05-20 | 2013-09-04 | 杨健 | Attractant capable of promoting cross pollination of fruit trees and improving fruit set percentage of fruit trees |
| CN104430584A (en) * | 2014-12-03 | 2015-03-25 | 红河学院 | Guava fruit fly attractant |
Non-Patent Citations (1)
| Title |
|---|
| 5种化合物对家蝇的引诱效果研究;莫晓畅;《中国媒介生物学及控制》;20130831;第24卷(第4期);第330-331页 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104782584A (en) | 2015-07-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107135950B (en) | Cultivation method for quickly obtaining lycium ruthenicum regenerated seedlings | |
| CN100581353C (en) | Artificial rapid reproduction method for rutaceae zanthoxylum plant zanthoxylum dissitum Hemsl | |
| Ray et al. | In vitro regeneration of brinjal (Solanum melongena L.) | |
| Kalimuthu et al. | In vitro micropropagation of Musa sapientum L.(Cavendish Dwarf) | |
| CN106614746A (en) | Passiflora edulis sims rapid propagation culture solution and using method thereof | |
| Mahadev et al. | An efficient protocol for large-scale plantlet production from male floral meristems of Musa spp. cultivars Virupakshi and Sirumalai | |
| CN109122313A (en) | A kind of culture medium and method for making wing calyx dendrobium nobile seed quickly breed seedling | |
| CN104304694B (en) | Dichocrocis punctiferalis man-made feeds, preparation method and continuously rearing method | |
| CN108040875A (en) | A kind of screening collocation method of the culture medium of efficient induction embryo callus | |
| CN108077068A (en) | A kind of rapid propagation method of the sterile seedling of Kiwi berry | |
| CN103766324B (en) | Fructus Jujubae garden utilizes the method for red neck normal room cocoon honeybee Biological control green plant bug | |
| CN108142283A (en) | A kind of tissue culture and rapid propagation method of acer catalpifolium | |
| CN104782584B (en) | A kind of mango pollination fly draws fly matrix and its application | |
| Behera et al. | Regeneration of Plantlet of Water Yam (Dioscorea oppositifoliaaL.) through In Vitro Culture from Nodal Segments | |
| Gami et al. | Microtuberization, minitubers formation and in vitro shoot regeneration from bud sprout of potato (Solanum tuberosum L.) cultivar K. badshah | |
| CN108142284A (en) | A kind of tissue culture and rapid propagation method of five leaflets maple | |
| Redmond et al. | Culture of Sargassum in Korea: Techniques and Potential for Culture in the US | |
| CN106561452B (en) | A kind of method for building up of Chinese catalpa Embryogenic Callus Suspension Culture | |
| CN102210260A (en) | Method for getting excised embryo by virtue of intergeneric distant hybridization between radish and kohlrabi | |
| Oliveira et al. | In vitro conservation of diploid banana accessions | |
| Srichuay et al. | Callus Induction and Somatic Embryogenesis from Anther Cultures of Hevea brasiliensis Muell Arg. | |
| Ahmed Hasan et al. | Development of standard protocols for in vitro regeneration of some selected banana cultivars (Musa spp.) from India | |
| Aina et al. | Culture vessel and auxin treatments affect in vitro rooting and ex vitro survival of six Arachis paraguariensis genotypes | |
| CN108740611B (en) | Artificial feed for dichocrocis punctiferalis larvae, preparation method of artificial feed and dichocrocis punctiferalis breeding method | |
| Sadawarti et al. | Prospect of early planting of potato seed crop in central India |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190510 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |