CN104770766B - A kind of preparation method of sea cucumber Oyster Protein powder - Google Patents
A kind of preparation method of sea cucumber Oyster Protein powder Download PDFInfo
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- CN104770766B CN104770766B CN201510210336.5A CN201510210336A CN104770766B CN 104770766 B CN104770766 B CN 104770766B CN 201510210336 A CN201510210336 A CN 201510210336A CN 104770766 B CN104770766 B CN 104770766B
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- 239000000843 powder Substances 0.000 title claims abstract description 103
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- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 62
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 62
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- 239000011575 calcium Substances 0.000 claims abstract description 19
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- YWLAZODTXMQWKL-UHFFFAOYSA-N 2-hydroxypropane-1,2,3-tricarboxylic acid ozone Chemical compound O=[O+][O-].C(CC(O)(C(=O)O)CC(=O)O)(=O)O YWLAZODTXMQWKL-UHFFFAOYSA-N 0.000 description 1
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The present invention provides a kind of preparation method of sea cucumber Oyster Protein powder, comprises the following steps：Step 1: weighing sea cucumber meat and oyster meat by 1 1.2: 1 weight ratio, freeze, crush, freeze-dried powder is made；Step 2: adding the distilled water that temperature is 35 42 DEG C in the freeze-dried powder obtained to step 1, it is 5.5 6.5 to adjust pH value, adds papain, when insulation 58 is small, obtains enzymolysis liquid；Step 3: the enzymolysis liquid that step 2 is obtained is spray-dried, up to sea cucumber Oyster Protein powder.The sea cucumber oyster product that the present invention obtains is full of nutrition, but sea cucumber is warm-natured, the people for being not suitable for body heat eats, oyster is cold in nature, the people for being not suitable for trembled with fear eats, and combines both and a kind of product is made, can be adapted for the crowd of various different demands, and the oyster calcium powder of oyster shell making is also added into the present invention, enrich the nutrition of the present invention.
Description
Technical field
The present invention relates to a kind of sea cucumber oyster product.It is more particularly related to a kind of sea cucumber Oyster Protein powder
Preparation method.
Background technology
Sea cucumber contain protein, calcium, potassium, zinc, iron, selenium, outside manganese isoreactivity material, other active ingredients have sea in sea cucumber body
Ginseng is plain and forms Stichopus Japonicus Acidic Mucopolysaccharide by aminohexose, hexuronic acid and fucose etc., separately contains 18 kinds of amino acid and is free of courage
Sterol.Because sea cucumber is warm-natured, there is tonifying kidney and benefiting sperm, enriching yin to be good for sun, nourishing blood for moistening dryness, menstruation regulating are dispelled the tonifying both YIN and YANGs such as labor, nourishing the fetus profit production
Effect.Oyster also known as raw oyster, cold nature, has the effect of calming the liver, tranquillizing the mind by relieving convulsion, softening and resolving hard mass, convergence astringent method.Oyster
Rich in nutrition content, contains the metallic elements such as protein, vitamin A, acid amide element, riboflavin, Yi Jixin, potassium, calcium, iron.
But it is also less that both marine products are made a kind of technique of nutrition product at present, and people can not be met to sea
The craving of ginseng and oyster abundant nutrition, also influences the popularization of sea cucumber and oyster product.Importantly, sea cucumber and oyster have compared with
Big fishy smell, this has seriously affected the appetite of people, quite a few people can not be enjoyed the nutrition of sea cucumber and oyster.Cause
This, there is an urgent need for research and develop a kind of new sea cucumber oyster product.
The content of the invention
It is excellent it is an object of the invention to solve at least the above and/or defect, and provide at least to will be described later
Point.
It is by the preparation method of sea cucumber Oyster Protein powder that the present invention, which has a purpose, takes activated carbon except raw meat, sodium chloride
Ozone removes raw meat, citric acid ozone except raw meat and chrysanthemum juice remove raw meat, and obtaining sea cucumber oyster product does not have fishy smell, and has chrysanthemum fragrance.
In order to realize these purposes and further advantage according to the present invention, there is provided a kind of preparation of sea cucumber Oyster Protein powder
Method, comprises the following steps：
Step 1: weighing sea cucumber meat and oyster meat by 1-1.2: 1 weight ratio, freeze, crush, freeze-dried powder is made；
Step 2: adding the distilled water that temperature is 35-42 DEG C in the freeze-dried powder obtained to step 1, adjusting pH value is 5.5-
6.5, papain is added, when insulation 5-8 is small, obtains enzymolysis liquid；
Step 3: the enzymolysis liquid that step 2 is obtained is spray-dried, up to sea cucumber Oyster Protein powder.
Preferably, the preparation method of the sea cucumber Oyster Protein powder, in the step 1, is claimed by above-mentioned weight ratio
After taking sea cucumber meat and oyster meat, it is put into a glass container, it is 1.2-1.5 times of sea cucumber meat and oyster meat gross weight to add weight
Activated carbon, is uniformly mixed, then seals glass container, when standing 10-12 is small in 0-5 DEG C of environment, takes out.
Preferably, the preparation method of the sea cucumber Oyster Protein powder, after the step 1, further includes：
Step 1), the freeze-dried powder for taking step 1 to obtain, are fitted into cloth bag, and sealing, is put into a glass container, add quality
The sodium chloride solution that fraction is 7-10% blasts ozone into sodium chloride solution, extremely up to flooding cloth bag with ozone generator
Ozone content in sodium chloride solution is 0.2mg/L, and glass container is sealed, and when standing 3-4 is small, is used during standing
The ultrasonic irradiation of 20kHz, opens glass container, stands 20-30 minutes, takes out and extract cloth bag；
Cloth bag, be put into glass container by step 2), adds citric acid solution that mass fraction is 10-15% until by cloth
Bag floods, and ozone is blasted into citric acid solution with ozone generator, and the ozone content into citric acid solution is 0.2mg/L,
Glass container is sealed, when standing 4-5 is small, the ultrasonic irradiation of 20kHz is used during standing, opens glass container, is stood
20-30 minutes, take out and extract cloth bag；
Cloth bag, be placed again into glass container by step 3), adds distilled water until cloth bag is flooded, being heated to water temperature is
40-50 DEG C, keep water temperature 10-15 minutes, take out cloth bag and extract, replace distilled water, repeat the step at least three times.
Preferably, the preparation method of the sea cucumber Oyster Protein powder, after the step 2, further includes：
Chrysanthemum juice is added in the enzymolysis liquid obtained to step 2, the addition quality of the chrysanthemum juice is the enzymolysis liquid quality
1.5-2 times；
The preparation method of the chrysanthemum juice includes：
Step a, chrysanthemum is weighed, the water of 7-8 times of chrysanthemum quality is added, is extracted 15-20 minutes under 35-40 DEG C of water temperature, mistake
Filter, obtains the first residue and the first filtrate；
Step b, the water of 15-20 times of the first mass of residue is added into the first residue, 30- is extracted under 35-40 DEG C of water temperature
40 minutes, filtering, obtained the second residue and the second filtrate；
Step c, the water of 7-8 times of the second mass of residue is added into the second residue, and adds volume to add water volume
The cellulase of 0.2-0.5% and the pectase of 0.3-0.4%, extract 20-25 minutes under 35-40 DEG C of water temperature, and filtering, obtains the
Three residues and the 3rd filtrate；
Step d, the first filtrate, the second filtrate and the 3rd filtrate are mixed, it is 1.2- that heating, which is concentrated into mixture relative density,
1.3, obtain chrysanthemum juice.
Preferably, the preparation method of the sea cucumber Oyster Protein powder, in the step 2, the additive amount of distilled water
For 5-8 times of freeze-dried powder quality.
Preferably, the preparation method of the sea cucumber Oyster Protein powder, in the step 2, is used in enzymolysis process
The ultrasonic irradiation of 20kHz.
Preferably, the preparation method of the sea cucumber Oyster Protein powder, in the step 2, in enzymolysis process,
The initial mass fraction 0.5% of papain.
Preferably, the preparation method of the sea cucumber Oyster Protein powder, after the step 2, further includes：By enzyme
Solve liquid pressurize 15 minutes under the conditions of 800MPa, 20 DEG C.
Preferably, the preparation method of the sea cucumber Oyster Protein powder, in the step 3, enzymolysis liquid is dry with spraying
Dry machine is spray-dried, and it is 150 DEG C to set spray dryer inlet temperature, and outlet temperature is 50 DEG C.
Preferably, the preparation method of the sea cucumber Oyster Protein powder, after the step 3, further includes：
Oyster calcium powder is mixed into sea cucumber Oyster Protein powder；
The preparation method of the oyster calcium powder is：Oyster shell is put into 1200-1500 DEG C of high temperature furnace and calcines 5-8 points
Clock, is crushed with the pulverizer of 500 mesh after cooling, obtains oyster calcium powder.
The present invention includes at least following beneficial effect：
(1) sea cucumber and oyster are full of nutrition, but sea cucumber is warm-natured, and the people for being not suitable for body heat eats, and oyster is cold in nature, are not suitable for
The people of trembled with fear eats, and combines both and a kind of product is made, and can be adapted for the crowd of various different demands, and this hair
The oyster calcium powder of oyster shell making is also added into bright, the nutrition of the present invention is enriched, also provides one for the utilization of oyster shell
Kind new approaches, the in the present invention utilization of oyster shell will not cause the waste of raw material, reduce production cost.
(2) deodorization is carried out to sea cucumber meat and oyster meat with activated carbon first in the present invention, by activated carbon and sea cucumber meat and male
Oyster meat mixes, and allows the amount of activated carbon to be more than the gross weight of both meat, be so put into refrigerator stand 10-12 it is small when, allow activated carbon
The fishy smell of both meat is fully absorbed, being put into refrigerator can also avoid meat from rotting, when standing small more than 12, activated carbon adsorption
The effect of fishy smell has been lost.
(3) present invention sea cucumber meat and oyster meat are freezed, freeze-dried powder is then made, this with heated drying then crush compared with,
Avoid nutrient component damages.Freeze-dried powder is also beneficial to carry out it deodorant, is sealed freeze-dried powder with cloth bag in the present invention, so
After be sequentially placed into sodium chloride solution and citric acid solution, the fishy smell material in freeze-dried powder is fully contacted with solution, and
Fully separate out, improve deodorization effect.Ozone is passed through in the solution, and can aoxidize fishy smell material using the oxidisability of ozone makes it
Water or volatilization are dissolved in, ozone is removed into raw meat with sodium chloride except raw meat, citric acid are except raw meat combines, fishy smell material can be made to go completely
Remove.Wherein, the movement velocity of each particle in solution can be promoted with the ultrasonic irradiation of 20kHz, promote fishy smell material quickly to analyse
Go out, also promote ozone molecule to enter cloth bag oxidation fishy smell material.Make ozone molten in sodium chloride solution and citric acid in the present invention
The content of liquid removes raw meat effect for 0.2mg/L with best, can make ozone part residual in the solution higher than 0.2mg/L, harm
The health of operator, and the amount of activated material in freeze-dried powder can be aoxidized, reduce the nutritive value of product.
(4) present invention further adds chrysanthemum juice also into enzymolysis liquid, contains a large amount of Flavonoid substances in chrysanthemum juice, has
Good removes raw meat effect, and substantial amounts of aromatic substance also plays the role of covering fishy smell in chrysanthemum juice, of the invention by chrysanthemum juice
Mix with enzymolysis liquid, be then spray-dried together, the product that can make has no fishy smell, and has chrysanthemum fragrance, improves production
The mouthfeel of product, is adapted to the needs of various crowds.The addition quality of chrysanthemum juice is 1.5 times of enzymolysis liquid in the present invention, obtained production
Product are without any fishy smell, and with strong chrysanthemum perfume, and when the addition quality of chrysanthemum juice is less than 1.5 times of enzymolysis liquid, chrysanthemum is fragrant
Taste is less obvious, and has slight fishy smell.
(5) by enzymolysis liquid, pressurize 15 minutes, the high pressure of 800MPa can make more in enzymolysis liquid under the conditions of 800MPa, 20 DEG C
Remaining enzyme inactivation, and germ therein and virus can be killed, compared to other enzyme deactivation sterilization methods, having does not reduce product nutrition
The advantages of value.
Further advantage, target and the feature of the present invention embodies part by following explanation, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Embodiment
With reference to example, the present invention is described in further detail, to make those skilled in the art with reference to specification text
Word can be implemented according to this.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein do not allot one or more
The presence or addition of a other elements or its combination.
<Example 1>
A kind of preparation method of sea cucumber Oyster Protein powder, comprises the following steps：
Step 1: weighing sea cucumber meat and oyster meat by 1: 1 weight ratio, freeze, crush, freeze-dried powder is made；
Step 2: adding the distilled water that temperature is 35 DEG C in the freeze-dried powder obtained to step 1, it is 5.5 to adjust pH value, is added
Enter papain, when insulation 5 is small, obtain enzymolysis liquid；
Step 3: the enzymolysis liquid that step 2 is obtained is spray-dried, up to sea cucumber Oyster Protein powder.
The preparation method of the sea cucumber Oyster Protein powder, in the step 1, sea cucumber meat is weighed by above-mentioned weight ratio
After oyster meat, it is put into a glass container, adds weight as sea cucumber meat and the activated carbon of 1.2 times of oyster meat gross weight, mixing
Uniformly, then glass container is sealed, when standing 10 is small in 0 DEG C of environment, taken out.
The preparation method of the sea cucumber Oyster Protein powder, after the step 1, further includes：
Step 1), the freeze-dried powder for taking step 1 to obtain, are fitted into cloth bag, and sealing, is put into a glass container, add quality
The sodium chloride solution that fraction is 7% blasts ozone into sodium chloride solution, to chlorination up to flooding cloth bag with ozone generator
Ozone content in sodium solution is 0.2mg/L, and glass container is sealed, when standing 3 is small, the surpassing with 20kHz during standing
Sound wave irradiates, and opens glass container, stands 20 minutes, takes out and extract cloth bag；
Cloth bag, be put into glass container by step 2), adds citric acid solution that mass fraction is 10% until by cloth bag
Flood, ozone is blasted into citric acid solution with ozone generator, the ozone content into citric acid solution is 0.2mg/L, will
Glass container seals, and when standing 4 is small, the ultrasonic irradiation of 20kHz is used during standing, opens glass container, stands 20 points
Clock, takes out and extracts cloth bag；
Cloth bag, be placed again into glass container by step 3), adds distilled water until cloth bag is flooded, being heated to water temperature is
40 DEG C, keep water temperature 10 minutes, take out cloth bag and extract, replace distilled water, repeat the step at least three times.
The preparation method of the sea cucumber Oyster Protein powder, after the step 2, further includes：
Chrysanthemum juice is added in the enzymolysis liquid obtained to step 2, the addition quality of the chrysanthemum juice is the enzymolysis liquid quality
1.5 times；
The preparation method of the chrysanthemum juice includes：
Step a, chrysanthemum is weighed, the water of 7 times of chrysanthemum quality is added, is extracted 15 minutes under 35 DEG C of water temperatures, filters, obtains first
Residue and the first filtrate；
Step b, the water of 15 times of the first mass of residue is added into the first residue, is extracted 30 minutes under 35 DEG C of water temperatures, mistake
Filter, obtains the second residue and the second filtrate；
Step c, the water of 7 times of the second mass of residue is added into the second residue, and adds volume to add water volume 0.2%
Cellulase and 0.3% pectase, extracted 20 minutes under 35 DEG C of water temperatures, filter, obtain the 3rd residue and the 3rd filtrate；
Step d, the first filtrate, the second filtrate and the 3rd filtrate are mixed, it is 1.2 that heating, which is concentrated into mixture relative density,
Obtain chrysanthemum juice.
The preparation method of the sea cucumber Oyster Protein powder, in the step 2, the additive amount of distilled water is freeze-dried powder
5 times of quality.
Preferably, the preparation method of the sea cucumber Oyster Protein powder, in the step 2, is used in enzymolysis process
The ultrasonic irradiation of 20kHz.
The preparation method of the sea cucumber Oyster Protein powder, in the step 2, in enzymolysis process, papain
Initial mass fraction 0.5%.
The preparation method of the sea cucumber Oyster Protein powder, after the step 2, further includes：Enzymolysis liquid is existed
800MPa, pressurize 15 minutes under the conditions of 20 DEG C.
The preparation method of the sea cucumber Oyster Protein powder, in the step 3, enzymolysis liquid is carried out with spray dryer
Spray drying, it is 150 DEG C to set spray dryer inlet temperature, and outlet temperature is 50 DEG C.
The preparation method of the sea cucumber Oyster Protein powder, after the step 3, further includes：
Oyster calcium powder is mixed into sea cucumber Oyster Protein powder；
The preparation method of the oyster calcium powder is：Oyster shell is put into 1200 DEG C of high temperature furnace and is calcined 5 minutes, after cooling
Crushed with the pulverizer of 500 mesh, obtain oyster calcium powder.
<Example 2>
A kind of preparation method of sea cucumber Oyster Protein powder, comprises the following steps：
Step 1: weighing sea cucumber meat and oyster meat by 1.2: 1 weight ratio, freeze, crush, freeze-dried powder is made；
Step 2: adding the distilled water that temperature is 42 DEG C in the freeze-dried powder obtained to step 1, it is 6.5 to adjust pH value, is added
Enter papain, when insulation 8 is small, obtain enzymolysis liquid；
Step 3: the enzymolysis liquid that step 2 is obtained is spray-dried, up to sea cucumber Oyster Protein powder.
Preferably, the preparation method of the sea cucumber Oyster Protein powder, in the step 1, is claimed by above-mentioned weight ratio
After taking sea cucumber meat and oyster meat, it is put into a glass container, adds weight as sea cucumber meat and the activity of 1.5 times of oyster meat gross weight
Charcoal, is uniformly mixed, then seals glass container, when standing 12 is small in 5 DEG C of environment, takes out.
The preparation method of the sea cucumber Oyster Protein powder, after the step 1, further includes：
Step 1), the freeze-dried powder for taking step 1 to obtain, are fitted into cloth bag, and sealing, is put into a glass container, add quality
The sodium chloride solution that fraction is 10% blasts ozone into sodium chloride solution, to chlorine up to flooding cloth bag with ozone generator
The ozone content changed in sodium solution is 0.2mg/L, and glass container is sealed, when standing 4 is small, with 20kHz's during standing
Ultrasonic irradiation, opens glass container, stands 30 minutes, takes out and extract cloth bag；
Cloth bag, be put into glass container by step 2), adds citric acid solution that mass fraction is 15% until by cloth bag
Flood, ozone is blasted into citric acid solution with ozone generator, the ozone content into citric acid solution is 0.2mg/L, will
Glass container seals, and when standing 5 is small, the ultrasonic irradiation of 20kHz is used during standing, opens glass container, stands 30 points
Clock, takes out and extracts cloth bag；
Cloth bag, be placed again into glass container by step 3), adds distilled water until cloth bag is flooded, being heated to water temperature is
50 DEG C, keep water temperature 15 minutes, take out cloth bag and extract, replace distilled water, repeat the step at least three times.
The preparation method of the sea cucumber Oyster Protein powder, after the step 2, further includes：
Chrysanthemum juice is added in the enzymolysis liquid obtained to step 2, the addition quality of the chrysanthemum juice is the enzymolysis liquid quality
2 times；
The preparation method of the chrysanthemum juice includes：
Step a, chrysanthemum is weighed, the water of 8 times of chrysanthemum quality is added, is extracted 20 minutes under 40 DEG C of water temperatures, filters, obtains first
Residue and the first filtrate；
Step b, the water of 20 times of the first mass of residue is added into the first residue, is extracted 40 minutes under 40 DEG C of water temperatures, mistake
Filter, obtains the second residue and the second filtrate；
Step c, the water of 8 times of the second mass of residue is added into the second residue, and adds volume to add water volume 0.5%
Cellulase and 0.4% pectase, extracted 25 minutes under 40 DEG C of water temperatures, filter, obtain the 3rd residue and the 3rd filtrate；
Step d, the first filtrate, the second filtrate and the 3rd filtrate are mixed, it is 1.3 that heating, which is concentrated into mixture relative density,
Obtain chrysanthemum juice.
The preparation method of the sea cucumber Oyster Protein powder, in the step 2, the additive amount of distilled water is freeze-dried powder
8 times of quality.
The preparation method of the sea cucumber Oyster Protein powder, in the step 2, the surpassing with 20kHz in enzymolysis process
Sound wave irradiates.
The preparation method of the sea cucumber Oyster Protein powder, in the step 2, in enzymolysis process, papain
Initial mass fraction 0.5%.
The preparation method of the sea cucumber Oyster Protein powder, after the step 2, further includes：Enzymolysis liquid is existed
800MPa, pressurize 15 minutes under the conditions of 20 DEG C.
The preparation method of the sea cucumber Oyster Protein powder, in the step 3, enzymolysis liquid is carried out with spray dryer
Spray drying, it is 150 DEG C to set spray dryer inlet temperature, and outlet temperature is 50 DEG C.
The preparation method of the sea cucumber Oyster Protein powder, after the step 3, further includes：
Oyster calcium powder is mixed into sea cucumber Oyster Protein powder；
The preparation method of the oyster calcium powder is：Oyster shell is put into 1500 DEG C of high temperature furnace and is calcined 8 minutes, after cooling
Crushed with the pulverizer of 500 mesh, obtain oyster calcium powder.
<Example 3>
A kind of preparation method of sea cucumber Oyster Protein powder, comprises the following steps：
Step 1: weighing sea cucumber meat and oyster meat by 1: 1 weight ratio, freeze, crush, freeze-dried powder is made；
Step 2: adding the distilled water that temperature is 40 DEG C in the freeze-dried powder obtained to step 1, it is 6 to adjust pH value, is added
Papain, when insulation 6 is small, obtains enzymolysis liquid；
Step 3: the enzymolysis liquid that step 2 is obtained is spray-dried, up to sea cucumber Oyster Protein powder.
The preparation method of the sea cucumber Oyster Protein powder, in the step 1, sea cucumber meat is weighed by above-mentioned weight ratio
After oyster meat, it is put into a glass container, adds weight as sea cucumber meat and the activated carbon of 1.5 times of oyster meat gross weight, mixing
Uniformly, then glass container is sealed, when standing 12 is small in 3 DEG C of environment, taken out.
The preparation method of the sea cucumber Oyster Protein powder, after the step 1, further includes：
Step 1), the freeze-dried powder for taking step 1 to obtain, are fitted into cloth bag, and sealing, is put into a glass container, add quality
The sodium chloride solution that fraction is 8% blasts ozone into sodium chloride solution, to chlorination up to flooding cloth bag with ozone generator
Ozone content in sodium solution is 0.2mg/L, and glass container is sealed, when standing 4 is small, the surpassing with 20kHz during standing
Sound wave irradiates, and opens glass container, stands 25 minutes, takes out and extract cloth bag；
Cloth bag, be put into glass container by step 2), adds citric acid solution that mass fraction is 12% until by cloth bag
Flood, ozone is blasted into citric acid solution with ozone generator, the ozone content into citric acid solution is 0.2mg/L, will
Glass container seals, and when standing 5 is small, the ultrasonic irradiation of 20kHz is used during standing, opens glass container, stands 25 points
Clock, takes out and extracts cloth bag；
Cloth bag, be placed again into glass container by step 3), adds distilled water until cloth bag is flooded, being heated to water temperature is
45 DEG C, keep water temperature 12 minutes, take out cloth bag and extract, replace distilled water, repeat the step at least three times.
The preparation method of the sea cucumber Oyster Protein powder, after the step 2, further includes：
Chrysanthemum juice is added in the enzymolysis liquid obtained to step 2, the addition quality of the chrysanthemum juice is the enzymolysis liquid quality
1.8 times；
The preparation method of the chrysanthemum juice includes：
Step a, chrysanthemum is weighed, the water of 8 times of chrysanthemum quality is added, is extracted 18 minutes under 38 DEG C of water temperatures, filters, obtains first
Residue and the first filtrate；
Step b, the water of 18 times of the first mass of residue is added into the first residue, is extracted 30-40 minutes under 38 DEG C of water temperatures,
Filtering, obtains the second residue and the second filtrate；
Step c, the water of 8 times of the second mass of residue is added into the second residue, and adds volume to add water volume 0.3%
Cellulase and 0.3% pectase, extracted 22 minutes under 38 DEG C of water temperatures, filter, obtain the 3rd residue and the 3rd filtrate；
Step d, the first filtrate, the second filtrate and the 3rd filtrate are mixed, it is 1.2 that heating, which is concentrated into mixture relative density,
Obtain chrysanthemum juice.
The preparation method of the sea cucumber Oyster Protein powder, in the step 2, the additive amount of distilled water is freeze-dried powder
6 times of quality.
The preparation method of the sea cucumber Oyster Protein powder, in the step 2, the surpassing with 20kHz in enzymolysis process
Sound wave irradiates.
The preparation method of the sea cucumber Oyster Protein powder, in the step 2, in enzymolysis process, papain
Initial mass fraction 0.5%.
The preparation method of the sea cucumber Oyster Protein powder, after the step 2, further includes：Enzymolysis liquid is existed
800MPa, pressurize 15 minutes under the conditions of 20 DEG C.
The preparation method of the sea cucumber Oyster Protein powder, in the step 3, enzymolysis liquid is carried out with spray dryer
Spray drying, it is 150 DEG C to set spray dryer inlet temperature, and outlet temperature is 50 DEG C.
The preparation method of the sea cucumber Oyster Protein powder, after the step 3, further includes：
Oyster calcium powder is mixed into sea cucumber Oyster Protein powder；
The preparation method of the oyster calcium powder is：Oyster shell is put into 1400 DEG C of high temperature furnace and is calcined 6 minutes, after cooling
Crushed with the pulverizer of 500 mesh, obtain oyster calcium powder.
In order to illustrate the effect of the present invention, it is as follows that inventor provides comparative experiments：
<Comparative example 1>
Ozone is not passed through into sodium chloride solution and citric acid solution, remaining parameter is with identical in example 3, technique
Process is also identical.
<Comparative example 2>
When being passed through ozone into sodium chloride solution and citric acid solution, it is 0.1mg/L to make the content of ozone in the solution,
For remaining parameter with identical in example 3, technical process is also identical.
<Comparative example 3>
Chrysanthemum juice is added not into enzymolysis liquid, remaining parameter is with identical in example 3, technical process also complete phase
Together.
<Comparative example 4>
When adding chrysanthemum juice into enzymolysis liquid, the addition quality of chrysanthemum juice is 1.2 times of enzymolysis liquid quality.Remaining parameter
With identical in example 3, technical process is also identical.
1 each example of table is contrasted with each comparative example
| Product odour | |
| Example 1 | Delicate fragrance |
| Example 2 | Delicate fragrance |
| Example 3 | Delicate fragrance |
| Comparative example 1 | Strong fishy smell |
| Comparative example 2 | Slight fishy smell |
| Comparative example 3 | Strong fishy smell |
| Comparative example 4 | Slight fishy smell |
From upper table 1, it can be seen that in example 3, since the content of ozone in the solution is 0.2mg/L, the addition of chrysanthemum juice
Quality is 1.8 times of enzymolysis liquid quality, and obtained product odour is delicate fragrance.Comparative example 1, comparative example 2 are compared with example 3, it is known that,
If not being passed through ozone, obtained product has stronger fishy smell, and intake also has slight fishy smell less than 0.2mg/L.
Comparative example 3, comparative example 4 are compared with example 3, it is known that, if being added without chrysanthemum juice, obtained product has stronger fishy smell,
1.5 times of addition deficiency enzymolysis liquid quality, also have slight fishy smell.As it can be seen that the present invention makes the content of ozone in the solution
For 0.2mg/L, the chrysanthemum juice that more than 1.5 times of addition enzymolysis liquid quality, removes raw meat effect with good, makes product delicate fragrance pleasant.
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed
With.It can be applied to various suitable the field of the invention completely., can be easily for those skilled in the art
Realize other modification.Therefore it is of the invention and unlimited under the universal limited without departing substantially from claim and equivalency range
In specific details and shown here as the example with description.
Claims (4)
1. a kind of preparation method of sea cucumber Oyster Protein powder, it is characterised in that comprise the following steps：
Step 1: press 1-1.2:1 weight ratio weighs sea cucumber meat and oyster meat, is put into a glass container, adds weight as sea
Join meat and the activated carbon of 1.2-1.5 times of oyster meat gross weight, be uniformly mixed, then seal glass container, in 0-5 DEG C of environment
When standing 10-12 is small, lyophilized, crushing, obtained freeze-dried powder are taken out；
Further include afterwards：
Freeze-dried powder is fitted into cloth bag by step 1), and sealing, is put into a glass container, adds the chlorination that mass fraction is 7-10%
Sodium solution blasts ozone into sodium chloride solution, the ozone into sodium chloride solution up to flooding cloth bag with ozone generator
Content is 0.2mg/L, and glass container is sealed, and when standing 3-4 is small, the ultrasonic irradiation of 20kHz is used during standing, is opened
Glass container, stands 20-30 minutes, takes out and extract cloth bag；
Cloth bag is put into glass container by step 2), and addition mass fraction is the citric acid solution of 10-15% until cloth bag is flooded
Not yet, ozone is blasted into citric acid solution with ozone generator, the ozone content into citric acid solution is 0.2mg/L, by glass
Glass container seals, and when standing 4-5 is small, the ultrasonic irradiation of 20kHz is used during standing, opens glass container, stands 20-30
Minute, take out and extract cloth bag；
Cloth bag is placed again into glass container by step 3), adds distilled water until cloth bag is flooded, it is 40-50 to be heated to water temperature
DEG C, keep water temperature 10-15 minutes, take out cloth bag and extract, replace distilled water, repeat the step at least three times；
Step 2: adding the distilled water that temperature is 35-42 DEG C in the product obtained to above-mentioned steps, adjusting pH value is 5.5-6.5,
Papain is added, insulation 5-8 obtains enzymolysis liquid when small, the wherein additive amount of distilled water is 5-8 times of freeze-dried powder quality, enzymolysis
During papain initial mass fraction 0.5%；Chrysanthemum juice is added into obtained enzymolysis liquid, the chrysanthemum juice adds
Enter 1.5-2 times that quality is the enzymolysis liquid quality；The preparation method of the chrysanthemum juice includes：
Step a) weighs chrysanthemum, adds the water of 7-8 times of chrysanthemum quality, extracts 15-20 minutes, filter under 35-40 DEG C of water temperature
First residue and the first filtrate；
Step b) adds the water of 15-20 times of the first mass of residue into the first residue, and 30-40 points are extracted under 35-40 DEG C of water temperature
Clock, filtering, obtains the second residue and the second filtrate；
Step c) adds the water of 7-8 times of the second mass of residue into the second residue, and adds volume to add water volume 0.2-
0.5% cellulase and the pectase of 0.3-0.4%, extract 20-25 minutes under 35-40 DEG C of water temperature, and filtering, it is residual to obtain the 3rd
Slag and the 3rd filtrate；
Step d) mixes the first filtrate, the second filtrate and the 3rd filtrate, and it is 1.2-1.3 that heating, which is concentrated into mixture relative density,
Obtain chrysanthemum juice；
Step 3: the enzymolysis liquid for adding chrysanthemum juice is spray-dried, up to sea cucumber Oyster Protein powder.
2. the preparation method of sea cucumber Oyster Protein powder as claimed in claim 1, it is characterised in that in the step 2,
The ultrasonic irradiation of 20kHz is used in enzymolysis process.
3. the preparation method of sea cucumber Oyster Protein powder as claimed in claim 1, it is characterised in that in the step 3, add
The enzymolysis liquid for entering chrysanthemum juice is spray-dried with spray dryer, and it is 150 DEG C to set spray dryer inlet temperature, outlet temperature
Spend for 50 DEG C.
4. the preparation method of sea cucumber Oyster Protein powder as claimed in claim 1, it is characterised in that after the step 3,
Further include：Oyster calcium powder is mixed into sea cucumber Oyster Protein powder；The preparation method of the oyster calcium powder is：Oyster shell is put into
Calcined 5-8 minutes in 1200-1500 DEG C of high temperature furnace, crushed after cooling with the pulverizer of 500 mesh, obtain oyster calcium powder.
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| CN105831388A (en) * | 2015-12-15 | 2016-08-10 | 浙江海洋学院 | Preparation method of taurine-rich protein powder |
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